scholarly journals Hydrolysis Optimization of Beneng Taro Tubers (Xantoshoma undipes K. Koch) as Bioethanol Raw Material

2021 ◽  
Vol 4 (2) ◽  
pp. 58-65
Author(s):  
Fenti Visiamah ◽  
Wasinton Simanjuntak
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Reducing Sugar ◽  
Raw Material ◽  
Optimum Amount ◽  
Hydrolysis Time ◽  
Hydrolysis Of

This study conducted to assess the most appropriate method or hydrolysis of beneng taro tuber to optimize the production of bioethanol to investigate the effect of pHs, times, and temperatures on reducing sugar produced. The concentration of reducing sugar was determined using a UV-Vis spectrophotometer.  The result obtained indicated that the optimum amount of reducing sugar (910,875 mg/L ) achieved at pH 10, hydrolysis time of 3 hours, and the temperature of 90 °C.  Fermentation of reducing sugar using the powdered bark of raru plant produced 0,18765 % (v/v) of bioethanol and fermentation using Saccharomyces cerevisiae produced 0,2116 % (v/v) of bioethanol.  

Reducing Sugar Production from Agricultural Wastes by Acid Hydrolysis

2016 ◽  
Vol 675-676 ◽  
pp. 31-34
Author(s):  
Achara Kleawkla ◽  
Pannarai Chuenkruth
Keyword(s):  
Sulfuric Acid ◽  
Corn Stover ◽  
Agricultural Waste ◽  
Sugar Content ◽  
Reducing Sugar ◽  
Agricultural Wastes ◽  
Raw Material ◽  
Hydrolysis Time ◽  
Industrial Processing ◽  
Hydrolysis Of

Sugar is very important raw material of many industries such as food, beverage and renewable energy. In this research, pretreatment and hydrolysis of agricultural wastes to produce reducing sugars for an ethanol production were investigated. The rice stalk and corn stover from agricultural wastes were firstly pretreated with sodium hydroxide at 121 °C in different time as 20 30 and 40 minutes for removal of lignin. After that, the condition of hydrolysis using sulfuric acid of the pretreated rice stalk and corn stover was optimized. The optimum condition that obtained the highest reducing sugar content from rice stalk and corn stover of 76.12 and 136.25 mg/ml were using 1.0 % v/v sulfuric acid at temperature of 121 °C for a hydrolysis time of 40 minutes. This research made value adding in the industrial processing, decrease environmental problem and reduce global warming crisis by optimized utilization of agricultural waste.

scholarly journals HIDROLISIS RUMPUT LAUT (Glacilaria sp.) MENGGUNAKAN KATALIS ENZIM DAN ASAM UNTUK PEMBUATAN BIOETANOL

Jurnal Kimia ◽  
2016 ◽  
Author(s):  
Yohanes Armawan Sandi ◽  
Wiwik Susanah Rita ◽  
Yenni Ciawi
Keyword(s):  
Gas Chromatography ◽  
Hydrochloric Acid ◽  
Sulphuric Acid ◽  
Ethanol Concentration ◽  
Reducing Sugar ◽  
Optimum Concentration ◽  
Raw Material ◽  
Optimum Length ◽  
Hydrolysis Of

The aim of this research is to determine the effect of enzyme and acids concentration on the yield of glucose produced in the hydrolysis of Glacilaria sp. in the production of bioethanol. The concentrations of cellulase used were 200 units/mL, 400 units/mL, 600 units/mL, 800 units/mL and the concentration of sulphuric acid (H2SO4) and hydrochloric acid (HCl) used were 1%, 3%, 5%, 7% (w/v). The concentration of reduction sugar was determined using Anthrone and analyzed using UV-Vis spectrophotometry and the determination of ethanol concentration was carried out by using gas chromatography. The results showed that the contents of reducing sugar produced by sulphuric acid (H2SO4) hydrolysis were 26,19%; 36,69%; 41,40%; 45,0% (v/v), by hydrochloric acid (HCl) were 12,12%; 14,03%; 15,17%; 16,50% (v/v), and by cellulase enzyme were 46,15%; 46,73%; 47,68%; 48,25% (v/v). Optimum concentration of reducing sugar produced by hydrolysis using 800 units/mL cellulase was 48,25% (v/v). The optimum length of fermentation to produce bioethanol using Glacilaria sp. as raw material was 5 days. In the fermentation, inoculum with a concentrations of 5% and 10% (w/v) produced 0,85% and 1,51% (v/v) ethanol.

scholarly journals Effect of pepsin hydrolysis on antioxidant activity of jellyfish protein hydrolysate

2021 ◽  
Vol 302 ◽  
pp. 02010
Author(s):  
Pratchaya Muangrod ◽  
Wiriya Charoenchokpanich ◽  
Vilai Rungsardthong ◽  
Savitri Vatanyoopaisarn ◽  
Benjamaporn Wonganu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protein Hydrolysate ◽  
Inhibitory Effect ◽  
Raw Material ◽  
Degree Of Hydrolysis ◽  
Dpph Radical ◽  
Radical Scavengers ◽  
Hydrolysis Time ◽  
Hydrolysis Of ◽  
By Products

Edible jellyfish have been consumed as food for more than a century with offering high protein and crunchy texture. The pepsin hydrolysis of jellyfish protein yields jellyfish protein hydrolysate (ep-JPH), reported for potential bioactivities such as antioxidant activity or antihypertensive activities. Due to the substantial number of by-products generated from jellyfish processing, the by-products were then selected as a raw material of JPH production. This research aimed to evaluate the effect of the hydrolysis time of pepsin on the antioxidant activity of ep-JPH. The dried desalted jellyfish by-products powder was enzymatically hydrolysed by 5% (w/w) pepsin, and the hydrolysis time was varied from 6, 12, 18, and 24 h at 37oC. Results showed that increased hydrolysis time increased the degree of hydrolysis (DH) and inhibition of DPPH radical. The 24 h ep-JPH possessed the highest DH and the highest inhibitory effect of DPPH radical. The results demonstrated that, in this experiment, all ep-JPHs were DPPH radical scavengers, exhibiting different inhibition activities depending on DH values.

scholarly journals BIOETHANOL PRODUCTION FROM COCONUT HUSK USING THE WET GAMMA IRADIATION METHOD

2021 ◽  
Vol 14 (2) ◽  
pp. 43
Author(s):  
Putra Oktavianto ◽  
Risdiyana Setiawan ◽  
Ilhami Ariyanti ◽  
Muhammad Fadhil Jamil
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cellulose Hydrolysis ◽  
Raw Material ◽  
Bioethanol Production ◽  
Coconut Husk ◽  
Hydrolysis Process ◽  
Hydrolysis Of Cellulose ◽  
Ethanol Ethanol ◽  
Hydrolysis Of ◽  
Low Dosage

BIOETHANOL PRODUCTION FROM COCONUT HUSK USING the WET GAMMA IRRADIATION METHOD. The use of coconut husk has only been used as a material for making handicrafts such as ropes, brooms, mats, and others or just burned. The combustion of coconut husk can cause air pollution. In fact, coconut husk can be used as a raw material for bioethanol production so that the beneficial value of coconut husk will also increase. One way of bioethanol production from coconut husk is by irradiating the coconut husk. The coconut husk irradiation technique to be carried out in this study is the wet irradiation technique. Wet irradiation is carried out to accelerate the process of bioethanol production because at the time of irradiation, cellulose has been hydrolyzed and glucose has been formed so that it is more efficient in time and use of the material so that the cellulose hydrolysis process is not necessary. The coconut husk samples were wet because they were mixed with 4% NaOH and were irradiated using a gamma irradiator from STTN-BATAN Yogyakarta with a dose of 30 kGy and 50 kGy and 0 kGy (or without irradiation). Then the sample is fermented with the fungus Saccharomyces Cerevisiae from tape yeast to form ethanol. Ethanol is purified and then analyzed for concentrations using pycnometric and refractometric methods. The result is that the highest ethanol content is without irradiation (0 kGy), this is due to the low dosage used. However, the main point in this wet method research is evidence of hydrolysis of cellulose by the formation of gluoxane after irradiated wet coconut husk, and with Fehling A and B analysis, brown deposits are seen proving that glucose has been formed.

Hydrolysis of S. platensis Using Sulfuric Acid for Ethanol Production

2022 ◽  
Vol 1048 ◽  
pp. 451-458
Author(s):  
Megawati ◽  
Astrilia Damayanti ◽  
Radenrara Dewi Artanti Putri ◽  
Zuhriyan Ash Shiddieqy Bahlawan ◽  
Astika Arum Dwi Mastuti ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Ethanol Production ◽  
Raw Material ◽  
Effect Of Temperature ◽  
Second Step ◽  
Carbohydrate Composition ◽  
Hydrolysis Time ◽  
Glucose Yield ◽  
Hydrolysis Temperature ◽  
Hydrolysis Of

S. platensis is a microalga that contains carbohydrate composition of 30.21% which makes it potential to be used as raw material for ethanol production. Hydrolysis of S. platensis is the first step for converting its carbohydrates into monosaccharides. The second step is fermentation of monosaccharides into ethanol. This research aims to study the effect of temperature and microalgae concentration on the hydrolysis of S. platensis using sulfuric acid as catalyst. This research was conducted using 300 mL sulfuric acid of 2 mol/L, hydrolysis temperatures of 70, 80 and 90 °C, and microalgae concentrations of 20, 26.7, and 33.3 g/L. The effect of temperature is significant in the hydrolysis of S. platensis using sulfuric acid. At microalgae concentration of 20 g/L and hydrolysis time of 35 minutes, the higher the temperatures (70, 80, and 90 °C), the more the glucose yields would be (8.9, 13.5, and 22.9%). This temperature effect got stronger when the hydrolysis was running for 15 minutes. Every time the hydrolysis temperature increased by 10 °C, the glucose yield increased by 13.0% at microalgae concentration of 33.3 g/L. At temperature of 90 °C and time of 35 minutes, the higher the microalgae concentrations (20, 26.7, and 33.3 g/L), the higher the glucose yields would be (25.5, 27.7, and 28.2%). The highest glucose concentration obtained was 2.82 g/L at microalgae concentration of 33.3 g/L, temperature of 90 °C, and time of 35 minutes.

scholarly journals Enzymatic hydrolysis of bitter cassava and Gadung starches with different compositions at low temperature

2018 ◽  
Vol 156 ◽  
pp. 01009
Author(s):  
Hargono Hargono ◽  
Andri Cahyo Kumoro ◽  
Bakti Jos
Keyword(s):  
Enzymatic Hydrolysis ◽  
Low Temperature ◽  
Optimum Condition ◽  
Starch Content ◽  
Reducing Sugar ◽  
Hydrolysis Time ◽  
Bitter Cassava ◽  
Manihot Glaziovii ◽  
Hydrolysis Of ◽  
Granular Starch

The effect of compositions of bitter cassava (Manihot glaziovii) and gadung (Dioscorea hispida Dennst) starches on reducing sugar during hydrolysis using granular starch hydrolyzing enzyme (GSHE) was studied. All hydrolyses were conducted at concentration of substrate was 200 g.L-1, while concentration of enzyme was 1.5 % (w/w), during of hydrolysis time 24 h, at 30°C. Mass compositition of bitter cassava and gadung starches were 9:1 to 1:9 The increase gadung starch compositions will decrease the reducing sugar. The optimum condition of the process using concentration of substrate 200 g.L-1 with compositions of bitter cassava and gadung starches was 9:1 at 18 h. It was found that reducing sugar was 50.20 g.L-1. The concentration of reducing sugar mainly depend on starch content on bitter cassava, it is much bigger than the gadung starch.

scholarly journals Hydrolysis of Straw in Ionic Liquids with Acid as Catalyst under Microwave Irradiation

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Yuanjing Zhang ◽  
Gang Wei ◽  
Guangyi Yu ◽  
Ning Qiao
Keyword(s):  
Ionic Liquids ◽  
Microwave Irradiation ◽  
Reducing Sugar ◽  
Raw Material ◽  
Conventional Heating ◽  
Acid Catalysts ◽  
Mass Ratio ◽  
Corn Straw ◽  
Experimental Conditions ◽  
Hydrolysis Of

With ionic liquids as solvents and corn straw as raw material, different processes of lignocellulose pretreatment with acid as catalyst were studied under conventional heating/microwave irradiation and the reducing sugar was measured. The results indicated that acid can accelerate hydrolysis reaction of corn straw into reducing sugar with ionic liquids as solvent, and microwave irradiation was more efficient in pretreatment of corn straw than conventional heating. The influences of different acid catalysts, the concentration of acid, temperature, mass ratio of straw/[Bmim]Cl, and the amount of refill water were mainly tested, and the optimum experimental conditions are thus determined.

scholarly journals SELEKSI SENYAWA PENGHIDROLISIS UNTUK MENGHASILKAN GULA REDUKSI DARI LIMBAH KULIT ARI KEDELAI SEBAGAI BAHAN FERMENTASI BIOETANOL

Jurnal BIOMA ◽  
2017 ◽  
Vol 13 (1) ◽  
pp. 1-8
Author(s):  
Siska Handayani ◽  
Zulkifliani Zulkifliani ◽  
Adisyahputra Adisyahputra ◽  
Devitra Sakarani
Keyword(s):  
Block Design ◽  
Hydrolysis Product ◽  
Secondary Data ◽  
Reducing Sugar ◽  
Raw Material ◽  
Randomized Block Design ◽  
Two Factors ◽  
Post Hoc ◽  
Hydrolysis Of ◽  
Reducing Sugar Concentration

This study aims to determine the most effective compound used to hydrolyze soy husk waste to produce reducing sugar as raw material for bioethanol fermentation. The study was conducted at the Laboratory of Bioprocess PPPTMGB "LEMIGAS" in April-September 2015. The method used is experiment using a randomized block design consisting of two factors. The first factor is the type of compounds used in the process of hydrolysis, namely H2SO4, HCl, NaOH, and NH3. The second factor is the concentration of hydrolyze compound 0.2%, 0.4%. 0.6%, 0.8%, and 1% (v/v) and every treatment repeated 4 times. Parameters measured were content of reduced sugar hydrolysis product, and secondary data that content of cellulose and hemicellulose also the density of ethanol. Concentration of reducing sugar from hydrolysis of soybean husk is analyzed by two-way ANOVA test. ANOVA analysis result indicate that the best hydrolysis compounds in hydrolizing soybean husk is HCl with the optimum concentration is 0,4%. And there are interactions between treatment of compound used to hydrolyze as well as concentration on reducing sugar concentration (mg/mL) as product from soybean husk waste hydrolysis. Post-hoc test showed that HCl 0,4% produce the highest concentration of reducing sugar at 31.23 mg/mL.

Enhanced Hydrolysis of Hemicellulose in Corn Stalk by Alkali Pretreatment

2014 ◽  
Vol 496-500 ◽  
pp. 175-178
Author(s):  
Yan Quan Guo ◽  
Wen Bo Wang ◽  
Fan Gong Kong ◽  
Shou Juan Wang
Keyword(s):  
Reducing Sugar ◽  
Sugar Yield ◽  
Corn Stalk ◽  
Hydrolysis Time ◽  
Pretreatment Condition ◽  
Whole Process ◽  
Alkaline Pulping ◽  
Hydrolysis Temperature ◽  
Alkali Hydrolysis ◽  
Hydrolysis Of

Alkali hydrolysis was used as a method of decomposing hemicellulose in corn stalk in this paper. The effects of NaOH concentration, hydrolysis time and hydrolysis temperature in the pretreatment on the degradation of hemicellulose in corn stalk were investigated. In addition, the yields of reducing-sugar and alkali hydrolysis corn stalk were discussed. The experimental results showed that the process of alkali hydrolysis had positive effect on the removal of hemicellulose in corn stalk and the whole process almost finished in 3h. The maximum reducing-sugar yield was 7.2% by the pretreatment with 20% NaOH for 6 h at 90°C, and 6.6% under the condition of 15% NaOH for 3h at 90°C, which was slightly lower than the maximum reducing-sugar yield, but the alkali charge and hydrolysis time dramatically reduced. So it was clarified that the best pretreatment condition was 15% NaOH for 3h at 90°C, and also this method is easy to applied in the alkaline pulping process.

scholarly journals Effect of Ultrasound-Assisted on Sequential Peracetic Acid and Alkaline Peroxide Pretreatment to The Enzymatic Hydrolysis of Oil Palm Empty Fruit Bunch

2021 ◽  
Author(s):  
Dwini Normayulisa Putri ◽  
Meka Saima Perdani ◽  
Masafumi Yohda ◽  
Tania Surya Utami ◽  
Muhamad Sahlan ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oil Palm ◽  
Peracetic Acid ◽  
Enzyme Concentration ◽  
Reducing Sugar ◽  
Empty Fruit Bunch ◽  
Hydrolysis Time ◽  
Alkaline Peroxide ◽  
Hydrolysis Process ◽  
Hydrolysis Of

Abstract Enzymatic hydrolysis of oil palm empty fruit bunch (OPEFB) that has been pretreated by modified pretreatment has been investigated in this study. The OPEFB used was pretreated by using sequential peracetic acid – alkaline peroxide solution. As the modification method, the assistance of pretreatment by ultrasound was conducted, in order to increase the enzyme accessibility. Therefore, it enhances the production of reducing sugar on the hydrolysis process. Prior to hydrolysis process, OPEFB was initially treated by using peracetic acid solution, comprise of CH3COOH (> 99%) and H2O2 (30% w/w), assisted by ultrasound for 3 hours at 35oC. Afterwards, OPEFB was treated by using alkaline peroxide solution, comprise of NaOH (40% w/w) and H2O2 (35% w/w), assisted by ultrasound for 10 hours at 35oC. OPEFB that has been pretreated was then subjected to enzymatic hydrolysis process using cellulase enzyme, in order to convert cellulose content into reducing sugar. Enzymatic hydrolysis was carried out at 50oC in a shaker incubator with 150 rpm for 48 hours. In this study, the effect of different enzyme concentration and hydrolysis time towards the sugar concentration in modified-pretreated OPEFB was observed and analyzed. Three different concentrations of enzyme were used, including 1.25, 2.5, and 5 g/L, and reducing sugar concentrations were analyzed at 30 and 45 minutes, and 1, 2, 4, 6, 24, 30, and 48 hours. Based on results, enzyme concentration has a significant effect to the production of reducing sugar. The reducing sugar concentrations obtained at the end of the hydrolysis process were 8.48, 11.06, 19.16 g/L, at the enzyme concentrations of 1.25, 2.5, and 5 g/L, respectively. At any hydrolysis time, the highest sugar concentration has been achieved on the highest enzyme concentration of 5 g/L. Moreover, the effective hydrolysis time were achieved at 6 hours, at all concentration of enzyme, since the production of reducing sugar were insignificant after 6 hours. This study showed an increase in reducing sugar production by 8.25% in the hydrolysis process using OPEFB pretreated by modified pretreatment compared to the non-modified pretreatment.

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