scholarly journals Monitoring Number Of Colifom and Escherichia Coli on Drinking Water Refill as Pollution Bioindicator

Jurnal Biota ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 30-36
Author(s):  
Hoetary Tirta Amallia
Keyword(s):  
Water Pollution ◽  
Escherichia Coli ◽  
Drinking Water ◽  
Natural Resources ◽  
Human Development ◽  
Frequency Distribution ◽  
Quality Standards ◽  
Coliform Bacteria ◽  
Contaminated Water ◽  
E Coli

One of the very needed natural resources on our planet is water. A total of 70% of the Earth's surface is water utilized for human development such as drinking, irrigation and cultivating fish. Contaminated water will have a bad effect on all living creatures and their environment [7]. Sources of water pollution can be derived from manusi stool containing coliform bacteria. The research purposes will be monitoring colifom number and Escherichia coli on some samples of drinking water as a bioindicator of drinking water pollution considering the higher the population consuming drinking water refill. This resource is pure resource experiment that use design of resource descriptive analitic. The data obtained will be displayed in the frequency distribution table to see the figure of coliform and E.coli in refill drinking water. From the results of this study, of 20 samples of drinking water refill there were 14 unqualified samples where the coliform figures exceeded quality standards. And 5 positive samples of E. Coli bacteria

scholarly journals Escherichia coli O157:H7 in Drin Prevalence of Escherichia coli O157:H7 in Drink from Different Food Premises in Kota Samarahan Sarawak

2019 ◽  
Vol 2 (2) ◽  
pp. a13-19
Author(s):  
ELEXSON NILLIAN ◽  
AMIZA NUR ◽  
DIYANA NUR ◽  
AMIRAH ZAKIRAH ◽  
GRACE BEBEY
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Plain Water ◽  
Escherichia Coli O157 ◽  
Probable Number ◽  
E Coli ◽  
Preventive Actions ◽  
Stx1 Gene

Contamination of drinks with E. coli O157:H7 served in food premises such as restaurants can cause haemorrhagic colitis and haemolytic uremic syndrome to humans. The presence or absence of faecal pathogen was demonstrated using coliform group as indicator microorganisms. Therefore, this study was conducted to detect the presence of E. coli O157:H7 in drinking water from food restaurant premise in Kota Samarahan and Kuching to ensure safe and potable drinking water is served to the consumer. A total of thirty (n=30) drink samples including six types of each of the samples are cold plain water, iced tea, iced milo, syrup and iced milk tea. Most Probable Number (MPN) procedure was used in this study to enumerate the MPN values of coliform bacteria in each drink collected. A total of 53.33% (16/30) of the drink samples showed positive E. coli detection. Then, the PCR assay showed 6.25% (one out of 16 isolates) samples were positive and carried stx1 gene produced by E. coli O157:H7 in iced milo sample types. This study showed the drinks collected from food premises was contaminated with faecal contamination, which was not safe to drink by the consumer. Therefore, preventive actions should be taken to prevent foodborne illness outbreak in future

scholarly journals The rationale for introducing the indices «Generalized coliform bacteria» and «Escherichia coli» into the scheme of sanitary and microbiological control of water quality as indices of fecal contamination

2021 ◽  
Vol 99 (12) ◽  
pp. 1353-1359
Author(s):  
Angelika V. Zagainova ◽  
Galina M. Trukhina ◽  
Yury A. Rakhmanin ◽  
Tamara Z. Artemova ◽  
Marina A. Sukhina
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Drinking Water ◽  
Fecal Contamination ◽  
Coliform Bacteria ◽  
E Coli ◽  
Intestinal Infections ◽  
Safety Of Drinking Water ◽  
Microbiological Indicators

Introduction. The increasing bacterial contamination of water bodies requires an increase in water quality control’s reliability to ensure epidemic safety against waterborne infections. Therefore, researchers in both Russia and Europe came to the conclusion that it is necessary to search for indicator microorganisms that can more accurately suggest the presence of pathogens. microorganisms in water than traditional indicators. The aim of the study was to justify the introduction of indicator indices of fecal contamination “generalized coliform bacteria” and Escherichia coli to assess the safety of drinking water Material and methods. The article provides an analysis of domestic and international regulatory documents and literary materials regulating the quality of drinking water in terms of sanitary and microbiological indicators and assessment criteria. The results of many years of experimental and field research carried out by research organizations and practical organizations of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare are presented. Results. On the territory of Russia, “general coliform bacteria,” is one of the indicator indices chosen according to the fermentation of lactose, determine the safety of drinking water. The water does not take into account pathogenic bacteria (Salmonella, Shigella) and a number of lactose-negative opportunistic bacteria, causative agents of intestinal infections. The study of microorganisms isolated from the feces of patients confirms the frequency of occurrence of lactose-negative microorganisms to varying from 20 to 100% of strains. With an annual trend towards a decrease in the percentage of non-standard drinking water samples in terms of microbiological indices, general intestinal infections (GII) of unknown etiology increase, i.e. risk of GII. If the quality of drinking water does not correspond to thermotolerant coliform bacteria (TCB), 95% of samples contain E. coli. Therefore, the determination of E. coli more reliably indicates the intake of fresh fecal contamination and provides efficiency in taking measures to eliminate an unfavorable situation than TCB. Conclusion. Reasons are given for the introduction of more reliable microbiological indicators of water safety control, such as - “generalized coliform bacteria” with the preservation of the abbreviation GCB, combining both lactose-positive and lactose-negative bacteria, determined by the sign of glucose fermentation, negative oxidase test and negative stain according to Gram and E. coli as an indicator of recent faecal contamination, which will allow the assessment of water quality for a wide range of bacteria of the order Enterobacterials, corresponding to the modern taxonomy of Enterobacteriaceae NCBI, will ensure harmonization with international requirements and the safety of drinking water for the population.

scholarly journals DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Jurnal BIOMA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Rezki Rachmawati ◽  
Muzajjanah Muzajjanah ◽  
Yoswita Rustam
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Coliform Bacterium ◽  
Probable Number ◽  
E Coli ◽  
Biological Contamination ◽  
Drinking Water Samples

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

scholarly journals Analisis kepadatan bakteri coliform di Sungai Siak (sekitar perumahan sari residence Kota Pekanbaru)

Jurnal Zona ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 65-72
Author(s):  
Arief Mubyarso ◽  
Yusni Ikhwan Siregar ◽  
Sofyan Husein Siregar
Keyword(s):  
Water Pollution ◽  
Water Quality ◽  
Drinking Water ◽  
Government Regulation ◽  
Preliminary Test ◽  
Quality Standards ◽  
Coliform Bacteria ◽  
Survey Method ◽  
Faecal Coliforms ◽  
Water Quality Standards

Faecal coliforms are indicative bacteria of fecal contamination. If the bacteria are found in the waters so that the waters has been polluted and can not be used as a source of drinking  water.  The  research  has  been  conducted  from April  to  June  2014  at  Sari Residence Housing which located in Siak riverbanks by using survey method. Densitydata of water coliform bacteria the preliminary test and assertion test in the laboratory according to SNI 2897-2008. Data analysis is using ANOVA statistics and compared with the water quality standards according to the Indonesian Government Regulation No. 82 year 2001 on the Management of Water Quality and Water Pollution ControlClass I and Class II. The results showed that the density of coliform bacteria in the Siak River (around Sari Residence Housing Pekanbaru city) ranged between 11267-15650jml/ 100mL and pass the drinking water quality standards and require special handlingfor the use of water. Siak River water (around Sari Residence Housing Pekanbaru city)not feasible for consumption according to PP 82 year 2001 class I and II on the drinkingwater requirements of 1000 jml/100mL and 5000 jml/100m

scholarly journals Identifikasi Bakteri Escherichia coli dalam Air Minum Galon pada Kantin yang ada di Universitas Andalas Padang

2021 ◽  
Vol 10 (1) ◽  
pp. 23
Author(s):  
Muhammad Rayhan Braja Gitawama ◽  
Netti Suharti ◽  
Nora Harminarti
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Data Analysis ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Probable Number ◽  
E Coli ◽  
Drug Agency ◽  
Drinking Water Samples

The National Food and Drug Agency has controlled the refill drinking water in drinking water depots and also controlled food at school canteens ranging from elementary, junior high, to high school, but the National Food and Drug Agency never do food quality controlling in canteens at universities. Objectives: To identified the contamination by coliform and E. coli bacteria in drinking water at Andalas University canteen. Methods: This research was descriptive to identify coliform bacteria on  15 drinking water at the Andalas University faculty canteens.  Samples were taken directly using a sterile bottle, while data analysis using Most Probable Number  (MPN)  tables 5-1-1 and the presence of E. coli bacteria colonies from drinking water samples. Results: 9 of 15 water samples were contaminated by coliform bacteria with the highest MPN index of 240/100 ml that was found in 2 samples. From 9 samples containing coliform bacteria, all of them were found to contain E. coli bacteria. Conclusion: Most of the samples were contaminated by coliform and E. coli bacteria. Drinking water served using a kettle was more contaminated than drinking water served using gallons.Keywords: Coliform, Escherichia coli, MPN

scholarly journals Assessment of microbial contamination of drinking water with total coliform bacteria and Escherichia coli in the Bitola region

2019 ◽  
Vol 65 (2) ◽  
pp. 23-32
Author(s):  
Marta Nedelkova ◽  
Angela Delova ◽  
Tanja Petreska Ivanovska ◽  
Zoran Zhivikj ◽  
Lidija Petrushevska-Tozi
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Supply ◽  
Rural Areas ◽  
Membrane Filtration ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Quality And Safety ◽  
E Coli ◽  
Total Coliform Bacteria

The aim of this paper is to analyze the presence of Escherichia coli (E. coli) and total coliform bacteria (CB) in the drinking water of urban and rural areas of the Bitola region, as indicators for water quality and safety. All water in urban area is chlorinated, while at the same time the water in rural areas is non-chlorinated. The samples were analyzed according to the international standard method of membrane filtration MKC EN ISO 9308-1:2015. In all examined samples of drinking water in urban area, presence of E. coli and CB was not detected as a result of the disinfection of the water. On contrary, in all tested samples of the water from the rural water supply, presence of E. coli and CB was confirmed. Significant increase in coliform bacterial counts probably weather-related was found in the period from April to September. In addition, in the third quarter including July, August, and September, in many measurements, E. coli as an indicator of faecal contamination was identified in drinking water. In accordance with these findings and in order to provide safe drinking water, it is necessary to modernize the water supply for the population in rural areas, to disinfect permanently the drinking water and to apply regular laboratory controls which are a basic pre-condition. Otherwise, inappropriate management of the water systems can cause serious decrease in the quality and safety of the drinking water associated with an increased risk of appearance of the infectious diseases in people and hydric epidemic. Key words: drinking water, Escherichia coli, coliform bacteria, membrane filtration

scholarly journals Antibiotic Resistance Phenotypes among Escherichia coli Isolated from Sachet and Pipe-Borne Water from Selected Local Government Areas of Kaduna State, Nigeria

2020 ◽  
pp. 11-16
Author(s):  
Alalade O.M. ◽  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Antibiotic Resistance ◽  
Local Government ◽  
Pure Water ◽  
Health Concern ◽  
Coliform Bacteria ◽  
E Coli ◽  
Resistance Patterns ◽  
Sachet Water

The presence of coliform bacteria such as Escherichia coli (E. coli) in water used for drinking is of great public health concern due to the risk it poses to consumers. This risk is exacerbated when the bacteria are resistant to commonly used antibiotics. This study aimed at determining the antibiotic resistant phenotypes among E. coli isolated from sachet water (Pure water) and municipal pipe-borne water in selected local government areas of Kaduna state, Nigeria. A total of 105 water samples (69 sachet water and 36 municipal pipe-borne water) were subjected to bacterial isolation following standard methods and the isolated E. coli were subjected to antibiotic susceptibility testing to ten (10) commonly used antibiotics. The isolation rates of the organism were 14.4% and 22.9% in sachet and pipe-borne water respectively. The isolates were all (100%) susceptible to gentamicin, and also showed high susceptibility to amoxicillin-clavulanic acid (83.3%), chloramphenicol (94.5%), cefotaxime (94.5%) and sulphamethoxazole - trimethoprim (100%). On the other hand, 9 isolates (50%) showed various resistance patterns with 3 isolates being resistant to up to seven (7) antibiotics, with resistance patterns TE, NA, C, CIP, CTX, AMP, AML and TE, NA, AMC, SXT, CIP, AMP, AML. Eight out of the 9 resistant isolates showed resistance to Nalidixic acid and 7 showed resistance to Tetracycline. These results indicate that drinking water in the study area is a source of antibiotic resistance. It is obvious that more attention needs to be focused on ensuring the safety and potability of drinking water in the study area. Key words: Escherichia coli, Antibiotic resistance, sachet water, pipe-borne water

scholarly journals Biosensor Chip for Monitoring of Water Pollution as Novel Test Tool in Public Health: Proof of Principle

2021 ◽  
Vol 10 (5) ◽  
pp. 386-392
Author(s):  
Nadira Ibrišimović Mehmedinović ◽  
Aldina Kesić ◽  
Almir Šestan ◽  
Aida Crnkić ◽  
Mirza Ibrišimović
Keyword(s):  
Public Health ◽  
Water Pollution ◽  
Heavy Metals ◽  
Drinking Water ◽  
Microbial Community Composition ◽  
Life Cycles ◽  
Coliform Bacteria ◽  
Contaminated Water ◽  
Test Tool ◽  
Biosensor Chip

Humans are generally exposed to a variety of pollutions present in the air they breathe, the food they eat or in the water they drink. Some of the most dangerous pollutions are metals and heavy metals. These are naturally occurring substances which are harmless when present in the environment at low levels. However, due to many pollutants such as industry processes or war activities, the heavy metal concentration can exceed the limit of tolerance and become very toxic for the natural environment and living organisms in it, including humans. Unlike organic pollutants, the heavy metals (as ions and as particulate matter) once introduced into the environment cannot be biodegraded and remain there indefinitely. By rainfall these pollutants can be partially transferred from air or soil into the rivers and drinking water sources, where they accumulate in even higher toxic levels. The high concentrations of heavy metals in contaminated natural water reservoirs have an impact on the microbial community composition which resides there. This type of water pollution can cause the changes in life cycles of natural bacterial populations, influencing their metabolic processes and proliferation. The presence of pathogens in water is normally indirectly determined by the testing for “indicator organism” such as coliform bacteria. Coliforms are usually present in larger numbers in contaminated water and at the same time they are indicators of whether other pathogenic bacteria are present, too. In crisis situations, like war or some natural disasters, where trusted sources of drinking water are not available anymore, the military and residents of affected areas are forced to use some alternative water resources that cannot be tested for their microbial or metal contamination properly. Therefore, the existence of some fast test that would detect not only dangerous bacterial pathogens in water, but also the presence of metals and heavy metals as well, would be of great help and importance for the human health. Even though the number of pathogens can be drastically reduced by the boiling of water, the heavy metals are not destroyed by high temperature. Hence the main objective of our work was to optimize the biosensor chip for microbial detection in contaminated water that would serve at the same time as an indicator for the chemical composition of the water, such as presence of metals and heavy metals, with potential to be used as a novel test tool in public health.

scholarly journals DETEKSI BAKTERI Escherichia coli DALAM AIR MINUM ISI ULANG YANG DISTERILISASI ULTRAVIOLET DI WILAYAH KECAMATAN JAGAKARSA

Jurnal BIOMA ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 73
Author(s):  
Rezki Rachmawati ◽  
Muzajjanah Muzajjanah ◽  
Yoswita Rustam
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Water Samples ◽  
Coliform Bacteria ◽  
Most Probable Number ◽  
Coliform Bacterium ◽  
Probable Number ◽  
E Coli ◽  
Biological Contamination ◽  
Drinking Water Samples

Refill Water Depot is currently more widely circulated and used as an alternative drinking water supply by the public. However the still unclear about the quality of the drinking water refill generated primarily of biological content. Parameters of biological contamination in drinking water caused by the Escherichia coli and coliform bacterium. This study aims to identify E. coli and coliforms in drinking water refill. Refill drinking water samples obtained from 16 drinking water refill from Jagakarsa subdsitrict. The method used is descriptive. Refill drinking water samples was taken and tested in the MPN (Most Probable Number) method and then to be tested in identification of E. coli. The results of testing the drinking water refill obtained 15 samples positive for coliform bacteria. Samples were positive for E. coli bacteria that sample B.1 and F.2.

Detection of Coliforms in Drinking Water and its Effect on Human Health - A Review

2014 ◽  
Vol 17 ◽  
pp. 122-131 ◽  
Author(s):  
Partha Pal
Keyword(s):  
Water Pollution ◽  
Drinking Water ◽  
Pathogenic Bacteria ◽  
Total Coliform ◽  
Oral Route ◽  
Coliform Bacteria ◽  
The Public ◽  
E Coli ◽  
Quality Of Water ◽  
Public Health Protection

The coliform group has been used extensively as an indicator of water quality and has historically led to the public health protection concept. Total coliforms are a group of bacteria commonly found in the environment, for example in soil or vegetation, as well as the intestines of mammals, including humans. Total coliform bacteria are not likely to cause illness, but their presence indicates that the water supply may be vulnerable to contamination by more harmful microorganisms. Escherichia coli (E. coli) is the only member of the total coliform group of bacteria that is found only in the intestines of mammals, including humans. The presence of E. coli in water indicates recent fecal contamination and may indicate the possible presence of disease-causing pathogens, such as bacteria, viruses, and parasites. Although most strains of E. coli bacteria are harmless, certain strains, such as E.coli 0157:H7, may cause illness. About 80 % of communicable diseases in the world are waterborne. According to WHO estimate about 80 % of water pollution in developing country, like India is carried by domestic waste. In India 70 % of the water is seriously polluted and 75 % of illness and 80 % of the child mortality is attributed to water pollution. The improper management of water systems may cause serious problems in availability and quality of water. The major pathogenic bacteria responsible for water borne disease are spread by the faeco-oral route, in which water may play an intermediate role. The aim of this review is to examine methods currently in use for the detection of coliforms in drinking water and also to evaluate the possible health hazards associated with drinking water contaminated with coliforms.

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