scholarly journals Microscopic Characterization of Keji Beling Extract (Strobilanthes crispus L.) As Herbal Medicine Studies

Jurnal Biota ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 109-117
Author(s):  
Pramita Laksitarahmi Isrianto ◽  
Sonny Kristianto ◽  
Sukian Wilujeng
Keyword(s):  
Leaf Extract ◽  
Water Solubility ◽  
High Water ◽  
Test Analysis ◽  
Herbal Products ◽  
Ic50 Value ◽  
Strobilanthes Crispus ◽  
Dpph Method ◽  
Microscopic Morphology

Keji Beling (Strobilanthes crispus L.) is one of medicinal plants in Acanthaceae family. The leaves are the part that is often used as the herbal products such as; herbs, tea and the others. This study aims to determine the characteristics of the functional groups of organic compounds, mineral content and antioxidant activity in the leaf extract of Strobilanthes crispus L. using pottery. Test analysis in research using FTIR, SEM-EDX, XRF. The result of  showed the presence of CH alkenes, CH aromatic, C-O alcohol/ether/carboxylic acid/ester, C-N amine, NO2 Nitro compounds, O-H hydrogen bond alcohol /phenol and N-H amines/amides The mineral composition contained macro elements of Ca, K, P and S. While the identified micro minerals are Si, Fe, Mo, Sr, Mn, Ba, Cu, Zn, and Ni. The microscopic morphology of Strobilanthes crispus  L. leaf extract is in the form of porous solid particels, thus it is to have high water solubility. The results of the antioxidant test using the DPPH method obtained an IC50 value of 19.20 ppm which is included in the group of very active antioxidants

scholarly journals Aktivitas Antioksidan Ekstrak Etanol Daun Ganitri (Elaeocarpus Ganitrus Roxb.) dengan Metode DPPH (2,2 Difenil-1-Pikrilhidazil)

2021 ◽  
Vol 18 (1) ◽  
pp. 60-67
Author(s):  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Sadam Husein ◽  
Titi Pudji Rahayu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Antioxidant Compounds ◽  
Synthetic Antioxidant ◽  
Activity Test ◽  
Impaired Liver ◽  
Ic50 Value ◽  
Dpph Method

Free radicals are one of the cause of various diseases. The use of synthetic antioxidant compounds could prevent the effect of the free radicals, however may cause adverse effects on the human body such as impaired liver, lung, intestinal and poisoning. Therefore antioxidant from natural resources needs to be developed. The purpose of this research was to determine the antioxidant activity and IC50 value of the ethanol extract of ganitri (Elaeocarpus ganitrus Roxbs.) leaves. Ganitri leaf ethanol extract activity test was carried out using DPPH method with vitamin C as a standard. Antioxidant activity was determined as a decreas in the absorbance of DPPH at 517 nm wavelength after an addition of the extract with the concentrations of 20, 40, 80, and 100 ppm. The antioxidant acitivity measurement of the ganitri leaf extract showed that the linier regression equation obtained was y = 0.3669x + 29.546, r = 0.4573 while the IC50 value was 54,12 ppm. Based on the result, it is concluded that the ethanol extract of ganitri (Elaeocarpus ganitrus Roxb.) leaf showed was categorized as strong antioxidant.

Antioxidant and Anti-Melanogenic Activities of Pakoba Leaves (Syzygium sp) from North Celebes

2019 ◽  
Vol 2 (2) ◽  
pp. 79-83
Author(s):  
Dela Rosa ◽  
Catherine Roeroe ◽  
Agustina Susanti
Keyword(s):  
Antioxidant Activity ◽  
Crude Extract ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Chloroform Fraction ◽  
Aqueous Fraction ◽  
Ic50 Value ◽  
Dpph Method ◽  
Good Potential ◽  
Crude Methanol Extract

In this research the potential of Pakoba leaf extract to be used as antioxidant and skin whitening (anti-melanogenic agent) is investigated. The antioxidant activity of Pakoba leaves were studied using DPPH method and the result showed that the 80 percent methanol crude extract has strong antioxidant activity with an IC50 value of 22.66 ± 1.02 μg/ml. The aqueous fraction of the sample has an IC50 value of 53.30 ± 1.42 μg/ml, followed by n-butanol fraction (53.63 ± 1.45 μg/ml) and chloroform fraction (511.54 ± 1.59 μg/ml). The anti-melanogenic activity of the crude methanol extract showed IC50 value of 316.56 ± 11.04 μg/ml. Thus, it isconcluded that crude extract of Pakoba leaves shows good potential as the antioxidant source although it does not show good anti- melanogenic activity.

scholarly journals Formulation and Characterization of an Effervescent Hydrogen-Generating Tablet

2021 ◽  
Vol 14 (12) ◽  
pp. 1327
Author(s):  
Moritz Rosch ◽  
Kurt Lucas ◽  
Jozef Al-Gousous ◽  
Ulrich Pöschl ◽  
Peter Langguth
Keyword(s):  
Mechanical Properties ◽  
Oral Delivery ◽  
Water Solubility ◽  
Hydrogen Generation ◽  
High Water ◽  
Sweet Taste ◽  
Dry Granulation ◽  
Medical Gas ◽  
And Oxidative Stress

Hydrogen, as a medical gas, is a promising emerging treatment for many diseases related to inflammation and oxidative stress. Molecular hydrogen can be generated through hydrogen ion reduction by a metal, and magnesium-containing effervescent tablets constitute an attractive formulation strategy for oral delivery. In this regard, saccharide-based excipients represent an important class of potential fillers with high water solubility and sweet taste. In this study, we investigated the effect of different saccharides on the morphological and mechanical properties and the disintegration of hydrogen-generating effervescent tablets prepared by dry granulation. Mannitol was found to be superior to other investigated saccharides and promoted far more rapid hydrogen generation combined with acceptable mechanical properties. In further product optimization involving investigation of lubricant effects, adipic acid was selected for the optimized tablet, due to regulatory considerations.

scholarly journals Antioksidan Daun Kumpai Mahung (Eupathorium inulifolium H.B&K)

2021 ◽  
Vol 8 (1) ◽  
pp. 94
Author(s):  
Dwi Rizki Febrianti ◽  
Rakhmadhan Niah ◽  
Novia Ariani
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Secondary Metabolites ◽  
Traditional Medicine ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Endemic Plants ◽  
Ic50 Value ◽  
Dpph Method

Daun Kumpai Mahung (Eupathorium inulifolium H.B & K) merupakan salah satu tumbuhan endemik Kalimantan Selatan. Secara turun temurun digunakan sebagai obat tradisional Dayak Meratus sebagai obat diare, demam, dan malaria. Tanaman ini dicurigai memiliki nilai antioksidan tinggi karena mengandung metabolit skunder fenolik. Tujuan penelitian ini untuk mengetahui aktivitas antioksidan ekstrak metanol daun E. inulifolium serta nilai IC50-nya. Penelitian ini menggunakan metode DPPH dengan instrumen spektofotometri UV-vis dengan panjang gelombang 517 nm. Dari hasil perhitungan dan replikasi nilai IC50 yang didapat sebesar 38,9 ppm. Ekstrak daun E. inulifolium memiliki aktivitas antioksidan yang sangat kuat dalam meredam radikal bebas. Kata Kunci: Daun, Potensi Antioksidan, Endemik, IC50, Ekstrak Etanol, Fenolik Kumpai Mahung (Eupathorium inulifolium H.B & K) leaves are one of the endemic plants of South Kalimantan. From generation to generation it is used as a traditional medicine for Dayak Meratus as a medicine for diarrhea, fever, and malaria. This plant is suspected of having high antioxidant value because it contains phenolic secondary metabolites. The purpose of this study was to determine the antioxidant activity of the methanol extract of E. inulifolium leaves and its IC50 value. This study used the DPPH method with spectophotometer UV-vis instrument at wavelength of 517 nm. From the calculation and replication, the IC50 value obtained is 38.9 ppm. E. inulifolium leaf extract has very strong antioxidant activity in reducing free radicals. 

scholarly journals Gel Formulation of Jamblang Leaf Extract (Syzygium cumini L) Skeel and Antioxidant Activity

2020 ◽  
Vol 36 (05) ◽  
pp. 946-953
Author(s):  
Samsuar Samsuar ◽  
Dina Hanifa
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Syzygium Cumini ◽  
Adhesion Test ◽  
Activity Test ◽  
Accelerated Stability ◽  
Ic50 Value ◽  
Dpph Method ◽  
Dispersion Test ◽  
Negative Controls

In this paper we reported gel formulation that containing Jamblang Leaf extract (Syzygium cumini L) and activity test as antioxidant. The Jamblang Leaf plant contained polyphenols compound which function as antioxidants. The purpose of this study was to determine the antioxidant activity of gel formulation that containing jamblang leaf extract. Extraction of Jamblang Leaf were formulated with three varation of concentration and gel basis as negative controls. The gel formulation were evaluated for organoleptic observations, homogeneity, pH, viscosity, dispersion test, adhesion test, accelerated stability test for 4 weeks storage, and measurement of antioxidant activity was carried out using the DPPH method with wavelength of 519 nm. The results of gel formulation IC50 value shows the ability of a substance to inhibit 50% DPPH radical of 47.735 ppm. The gel extract of Jamblang Leaf has a very strong antioxidant activity.

Uji Antikanker dan Antioksidan Ekstrak Metanol Daun Pasote (Dysphania ambrosioides L.) Anticancer and Antioxidant Test of Methanol Extract of Epazote leaves (Dysphania ambrosioides L.)

2019 ◽  
Vol 9 (2) ◽  
pp. 102
Author(s):  
Praise Meningkas ◽  
Dingse Pandiangan ◽  
Febby Kandou
Keyword(s):  
Vitamin C ◽  
Mtt Assay ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Test Method ◽  
Ic50 Value ◽  
Dpph Method ◽  
Anti Cancer ◽  
Calculation Results ◽  
Anti Oxidant

Uji Antikanker dan Antioksidan Ekstrak Metanol Daun Pasote (Dysphania ambrosioides L.)Anticancer and Antioxidant Test of Methanol Extract of Epazote leaves (Dysphania ambrosioides L.) Praise Frena Maningkas1*), Dingse Pandiangan1), Febby Ester Fany Kandou1)1)Jurusan Biologi, FMIPA, UNSRAT, Manado*E-mail: [email protected] Diterima 10 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 ABSTRAK Tujuan penelitian ini adalah untuk mendapatkan informasi ilmiah mengenai potensi antikanker dan antioksidan ektrak metanol serbuk daun pasote. Pengujian antikanker dilakukan dengan metoda uji MTT pada sel leukemia P388.Uji antioksidan dari ekstrak metanol, ditentukan dengan Spektro UV-Vis pada panjang gelombang 517 nm dengan metoda DPPH. Hasil pengamatan dan pengukuran diuji dengan program Origin Lab untuk antikanker, dan program Excel untuk analisis data antioksidan. Ekstak metanol pasote memiliki aktivitas yang kuat sebagai antikanker. Hasil perhitungan didapat nilai IC50 ekstrak metanol adalah adalah 53,37µg/mL termasuk kategori kuat sebagai antikanker. Nilai IC50 Quersetin adalah 87,78 μg/mL termasuk kategori kuat juga karena <100 μg/mL. Hasil perhitungan didapat nilai IC50 ekstrak metanol adalah adalah 50,131 µg/mL termasuk kategori sangat kuat sebagai antioksidan. Nilai IC50  vitamin C 48,533 μg/mL termasuk kategori sangat kuat juga karena <50 μg/mL. Hal ini menunjukkan bahwa ektrak air daun pasote potensial untuk dijadikan antioksidan dan tidak berbeda nyata dengan antioksidan vitamin C. Hal ini menunjukkan bahwa ektrak metanol daun pasote potensial untuk dijadikan antikanker dan antioksidan.Kata kunci: Antikanker, Antioksidan, Leukemia P388, MTT assay, DPPH ABSTRACT The aim of this study was to obtain scientific information about the anti-cancer and anti-oxidant potential of methanol extract of pasote. Anticancer testing was carried out by the MTT test method on P388 leukemia cells. The antioxidant test of methanol extract was determined by UV-Vis spectroscopy at a wavelength of 517 nm using the DPPH method. The results of observations and measurements were tested with the Origin Lab program for anticancer, and the Excel program for analysis of antioxidant data. The calculation results showed IC50 value of methanol extract was 53.37 µg / mL which was in the strong category as anticancer. The IC50 Quercetin value was 87.78 μg / mL which was in the strong category because it was <100 μg / mL. The results of the research obtained were pasote methanol extract have strong activity as antioxidants. The calculation results obtained IC50 value of methanol extract was 50.131 µg / mL including a strong category as an antioxidant. The IC50 value of vitamin C 48.533 μg / mL was also in a strong category because it was <50 μg / mL. This showed that pasote leaf extract is potential to be used as an anti-oxidant and not significantly different from vitamin C anti-oxidant. Overall, this study showed that epazote leaf extract is potential to be used as an anti-cancer and anti-oxidant.Keywords: Anti-cancer, Anti-oxidant, Leukemia P388, MTT assay, DPPH.

scholarly journals Uji Antikanker dan Antioksidan Ekstrak Metanol Daun Pasote (Dysphania ambrosioides L.) Anticancer and Antioxidant Test of Methanol Extract of Epazote leaves (Dysphania ambrosioides L.)

2019 ◽  
Vol 9 (2) ◽  
pp. 102
Author(s):  
Praise Maningkas ◽  
Dingse Pandiangan ◽  
Febby Kandou
Keyword(s):  
Vitamin C ◽  
Mtt Assay ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Test Method ◽  
Ic50 Value ◽  
Dpph Method ◽  
Anti Cancer ◽  
Calculation Results ◽  
Anti Oxidant

Uji Antikanker dan Antioksidan Ekstrak Metanol Daun Pasote (Dysphania ambrosioides L.)Anticancer and Antioxidant Test of Methanol Extract of Epazote leaves (Dysphania ambrosioides L.) Praise Frena Maningkas1*), Dingse Pandiangan1), Febby Ester Fany Kandou1)1)Jurusan Biologi, FMIPA, UNSRAT, Manado*E-mail: [email protected] Diterima 10 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 ABSTRAK Tujuan penelitian ini adalah untuk mendapatkan informasi ilmiah mengenai potensi antikanker dan antioksidan ektrak metanol serbuk daun pasote. Pengujian antikanker dilakukan dengan metoda uji MTT pada sel leukemia P388.Uji antioksidan dari ekstrak metanol, ditentukan dengan Spektro UV-Vis pada panjang gelombang 517 nm dengan metoda DPPH. Hasil pengamatan dan pengukuran diuji dengan program Origin Lab untuk antikanker, dan program Excel untuk analisis data antioksidan. Ekstak metanol pasote memiliki aktivitas yang kuat sebagai antikanker. Hasil perhitungan didapat nilai IC50 ekstrak metanol adalah 53,37µg/mL termasuk kategori kuat sebagai antikanker. Nilai IC50 Quersetin adalah 87,78 μg/mL termasuk kategori kuat juga karena <100 μg/mL. Hasil perhitungan didapat nilai IC50 ekstrak metanol adalah adalah 50,131 µg/mL termasuk kategori sangat kuat sebagai antioksidan. Nilai IC50  vitamin C 48,533 μg/mL termasuk kategori sangat kuat juga karena <50 μg/mL. Hal ini menunjukkan bahwa ektrak air daun pasote potensial untuk dijadikan antioksidan dan tidak berbeda nyata dengan antioksidan vitamin C. Hal ini menunjukkan bahwa ektrak metanol daun pasote potensial untuk dijadikan antikanker dan antioksidan.Kata kunci: Antikanker, Antioksidan, Leukemia P388, MTT assay, DPPH ABSTRACT The aim of this study was to obtain scientific information about the anti-cancer and anti-oxidant potential of methanol extract of pasote. Anticancer testing was carried out by the MTT test method on P388 leukemia cells. The antioxidant test of methanol extract was determined by UV-Vis spectroscopy at a wavelength of 517 nm using the DPPH method. The results of observations and measurements were tested with the Origin Lab program for anticancer, and the Excel program for analysis of antioxidant data. The calculation results showed IC50 value of methanol extract was 53.37 µg / mL which was in the strong category as anticancer. The IC50 Quercetin value was 87.78 μg / mL which was in the strong category because it was <100 μg / mL. The results of the research obtained were pasote methanol extract have strong activity as antioxidants. The calculation results obtained IC50 value of methanol extract was 50.131 µg / mL including a strong category as an antioxidant. The IC50 value of vitamin C 48.533 μg / mL was also in a strong category because it was <50 μg / mL. This showed that pasote leaf extract is potential to be used as an anti-oxidant and not significantly different from vitamin C anti-oxidant. Overall, this study showed that epazote leaf extract is potential to be used as an anti-cancer and anti-oxidant.Keywords: Anti-cancer, Anti-oxidant, Leukemia P388, MTT assay, DPPH.

scholarly journals Synthesis and characterization of a hyper-branched water-soluble β-cyclodextrin polymer

2014 ◽  
Vol 10 ◽  
pp. 2586-2593 ◽  
Author(s):  
Francesco Trotta ◽  
Fabrizio Caldera ◽  
Roberta Cavalli ◽  
Andrea Mele ◽  
Carlo Punta ◽  
...  
Keyword(s):  
Water Solubility ◽  
High Water ◽  
Water Soluble ◽  
Critical Conditions ◽  
Molar Ratio ◽  
Water Soluble Polymer ◽  
Solvent Phase ◽  
New Material ◽  
Crucial Parameter

A new hyper-branched water-soluble polymer was synthesized by reacting β-cyclodextrin with pyromellitic dianhydride beyond the critical conditions that allow the phenomenon of gelation to occur. The molar ratio between the monomers is a crucial parameter that rules the gelation process. Nevertheless, the concentration of monomers in the solvent phase plays a key role as well. Hyper-branched β-cyclodextrin-based polymers were obtained performing the syntheses with excess of solvent and cross-linking agent, and the conditions for critical dilution were determined experimentally. A hyper-branched polymer with very high water solubility was obtained and fully characterized both as for its chemical structure and for its capability to encapsulate substances. Fluorescein was used as probe molecule to test the complexation properties of the new material.

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