scholarly journals Ekstrak Umbi Rumput Teki (Cyperus Rotundus) Sebagai Antibakteri Terhadap Staphylococcus Epidermidis Dan Propionibacterium Acnes

2018 ◽  
Vol 9 (2) ◽  
pp. 165-175
Author(s):  
Siti Nurjanah ◽  
Akhmad Rokiban ◽  
Erdy Irawan
Keyword(s):  
Antimicrobial Activity ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Positive Control ◽  
Negative Control ◽  
Cyperus Rotundus ◽  
Mean Diameter ◽  
Inhibition Zones ◽  
The Mean

The tuber of cyperus rotundus are used to tradisional medicine. C. rotundus known to contain compounds of secondary metabolites such as alkoloids, sineol, pinene, siperon, rotunal, siperenon, siperol, tannins, flavonoids and scientifically it has been believed to have antimicrobial activity. Empirically this plant are used as an antibacterial, antitumor, anticancer, and antiallergic. The purpose of this study were determining the inhibition of extract of Cyperus rotundus against tubers of Staphylococcus epidermidis and Propionibacterium acnes. In this study C. rotundus extracted by maceration using 70% ethanol. The extracts tested against S. epidermidis and P. acnes using disc blank method. The results obtained from the mean diameter of the inhibition zones of exstract at concentrations of 20%, 40%, 60%, 80%, and 100% on S. Epidermidis 8.03 mm, 11.43 mm, 15.04 mm, 15.83 mm and 19.98 mm respectively and P. acnes 11.59 mm, 13.35 mm, 17.17 mm, 20.44 mm, and 30.08 mm respectively Aquades used as negative control and erythromycin used as positive control. Testing were continued by determining the Minimum Inhibitory Concentration (MIC). The result of MIC for S. epidermidis was 15% and P. acnes was 11%. The analysis showed that the antibacterial activity of the tubers extract is bacteriostatic for S. epdermidis and bacterisid for P. acnes.

THE EFFECTIVENESS TEST OF MATOA (PometiaPinnata) ENDOPHYTIC BACTERIA TOWARDS BACTERIA NOSOCOMIAL INFECTION

2020 ◽  
Vol 1 (1) ◽  
pp. 37-40
Author(s):  
Denny Chandra Halid
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nosocomial Infection ◽  
Staphylococcus Epidermidis ◽  
Endophytic Bacteria ◽  
Positive Control ◽  
Negative Control ◽  
Bacterial Isolates ◽  
Series Design ◽  
Inhibition Zones ◽  
The One

This study aims to determine the effectiveness of Matoa (Pometiapin-nata) endophytic bacteria towards bacteria nosocomial infection namely Pseudomonas aeruginosa and Staphylococcus epidermidis. The subjects in the study were Matoa plant endophytic bacterial isolates on the stem (tw-igs). The positive control used is meropenem & negative control of aquades. This type of research uses quasi-experiments with a research design us-ing the One-Group Time-Series Design. The result of the study shows that there are 2 endophytic bacterial iso-lates in Matoa plants namely BEM 1 and BEM 2. Both endophytic bacterial isolates can kill and inhibits bacterial nosocomial infections Pseudomonas aeruginosa and Staphylococcus epider-midis with inhibition zones in the range of 16mm-22mm with a strong category very strong that it has the po-tential to be used as an antibacterial

scholarly journals 2. Anti-Bacterial Activity Of N-Hexane Extract Of Malacca Leave (Phyllanthus emblica) On Mice (Mus musculus) Inoculated By Staphylococcus epidermidis In Vivo

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Nada Sarah Syahputri ◽  
Nuzul Asmilia ◽  
Rinidar Rinidar ◽  
Amalia Sutriana ◽  
Fakhrurrazi Fakhrurrazi ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Positive Control ◽  
Negative Control ◽  
Hexane Extract ◽  
Phyllanthus Emblica ◽  
Treatment Groups ◽  
Significant Difference ◽  
Treatment Data ◽  
The Mean

Malacca plant (Phyllanthus emblica) is one of the medicinal plants. The purpose of this study was to determine the effect of n-hexane extract of Malacca (Phyllanthus emblica) leaves on the growth of Staphylococcus epidermidis bacteria in vivo. All mice were first induced by Staphylococcus epidermidis bacteria. Negative control (K1) was given aquadest, positive control (K2) was given ciproflaxacin suspension at doses of 20 mg/kg BW, while K3, K4, and K5 were given n-hexane extract of Malacca leave at dose of 100 mg/kg BW, 200 mg/kg BW, and 300 mg/kg BW. Respectively blood sampling was carried out on the 5th day after treatment. Data were analyzed using one-way analysis of variance (ANOVA). The results showed that the mean (± SD) number of bacterial colonies in K1 was 656x10² cfu/ml. The average number of bacterial colonies in K2 was 2328x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 100 mg/kg BW on K3 was 359,60x10² cfu/ml. The average number of bacterial colonies given n-hexane extract of malacca leave 200 mg/kg BW at K4 was 200x10² cfu/ml and the average number of bacterial colonies given n-hexane extract of malacca leave 300 mg/kg BW at K5 was 3483x10² cfu/ml. The results showed there were no significant difference among treatment groups (P 0.05). N-hexane extract of malacca leave was unable to inhibit the growth of Staphylococcus epidermidis bacteria in vivo

scholarly journals Evaluation of minimum inhibitory concentration and antimicrobial activity of basil extract (Ocimum basilicum L.) incorporated to the mouthwash

2014 ◽  
Vol 2 (5) ◽  
pp. 409
Author(s):  
Ana Carolina Marcantonio ◽  
Guilherme Oliveira ◽  
Eloisa Boeck ◽  
Thalita Pilon ◽  
Adilson Bernardi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Positive Control ◽  
Negative Control ◽  
Ocimum Basilicum ◽  
Verification Method ◽  
Microtiter Plates

AIM: This study has as aim to evaluate the minimum inhibitory concentration and the antimicrobial activity of basil extract incorporated to the mouthwash against the bacteria S.mutans. MATERIAL AND METHODS: For the study, the hydro alcoholic basil extract (Ocimum basilicum L.) incorporated to the mouthwash was used in order to evaluate the effect of this formulation on bacteria and its Minimum Inhibitory Concentration (MIC). As positive control, TBS + S. mutans was used; as negative control, only the TBS; the fluid hydro alcoholic basil extract 20% and the concentrated basil extract 12%, both incorporated to the mouthwash, were also evaluated. As MIC verification method and antimicrobial activity, the micro dilution was used in the concentrations: pure, 1/2, 1/4, 1/8, 1/16, 1/32, 1/64, 1/128; and carried out in triplicate. The microtiter plates were incubated and evaluated after 24 and 72hs. RESULTS: The results showed there was no antimicrobial activity of mouthwash associated to the fluid and concentrated basil extract. However, the mouthwash insulated showed antimicrobial activity only as pure; other dilutions did not presented the same result. CONCLUSION: Before the findings in this study, it is possible conclude that hydro alcoholic basil extract incorporated to the mouthwash did not present antimicrobial activity against the bacteria S. mutans.

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN SURUHAN (Peperomia pellucida L. Kunth) TERHADAP Propionibacterium acnes PENYEBAB JERAWAT

2020 ◽  
Vol 2 (1) ◽  
pp. 35-41
Author(s):  
Yufiradani Yufiradani ◽  
Delladari Mayefis ◽  
Hesti Marliza
Keyword(s):  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Skin Diseases ◽  
Antibacterial Properties ◽  
Positive Control ◽  
Negative Control ◽  
Leaf Extracts ◽  
Common Skin ◽  
The One ◽  
One Way Anova

Acne is one of the most common skin diseases that occur at all ages, especially in adolescents who are just experiencing puberty. One of the plants that has antibacterial properties is the leaves of suruhan. However untill now not found research of suruhan leaf used as an acne medicine. The aim of this study was to determine the antibacterial activity of suruhan leaf extracts on the growth of Propionibacterium acnes. The method used is disc diffusion by given 5 treatments starting with a concentration of 15%, 20%, 25%, positive control of clindamycin and negative control of aquadest.. The results obtained from leaf extracts were able to inhibit the growth of P. acnes bacteria in various concentrations. Suruhan leaf extracts at a concentration of 25% showed response resistance was greater than other concentrations. The one way Anova SPSS test showed p <0.05 which means that there were differences in the inhibitory concentration of various extracts on the growth of P. acnes that cause acne

scholarly journals ISOLASI LIKOPEN DARI BUAH TOMAT (Solanum lycopersicum L) dan UJI AKTIVITAS LIKOPEN TERHADAP BAKTERI Salmonella thypi

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Earlyna Sinthia Dewi ◽  
Aliefman Hakim ◽  
Lalu Rudyat Telly Savalas
Keyword(s):  
Fourier Transform ◽  
Wave Length ◽  
Extraction Process ◽  
Positive Control ◽  
Negative Control ◽  
Bacterial Activity ◽  
Inhibitory Zone ◽  
Solanum Lycopersicum L ◽  
Mean Diameter ◽  
The Mean

This research aims to know the effectiveness of lycopene antibacterial, through knowing bending zone lycopene towards salmonella thypi. This research has been success to isolate lycopene through extraction process used reflux method at 60oC temperature, using chloroform and methanol as antisolvent. Lycopene extract got 5,12 mg/100 g, analysis of functional groups by using spectroscopy Fourier Transform InfraRed (FTIR) detected C=C alkena alifatik at 1674,91 dan 1639,65 cm-1 wave length, C-H(CH3) detected at 1378,71 cm-1       wavelength, C-H  (stretching)  alifatikfunctional groups detected at 2853,12 cm-1 wave length, C-H alkena (stretching) detected at  2924,16 cm-1 wave length, and C-H alkena (bending) detected at  1498,86  cm-1 wave length. Inhibitory zone testing of salmonella thypi used paperdisc method with three repetitions. The mean diameter of the inhibitory zone of bacteria formed in positive control was 33,17 mm, negative control was 0 mm. Mean diameter of bacterial inhibitory zone at 3 % concentration with 3, 68 mm mean diameter and maximal Inhibitory zone at 50 % concentration with        15,12 mm mean diameter . The result of this research shown that lycopene extract has bacterial activity towards salmonella thypi Kewords: Isolation, Lycopene,  Salmonella thypi, Antibacterial

scholarly journals Antiacne-causing Bacteria, Antioxidant, Anti-Tyrosinase, Anti-Elastase and Anti-Collagenase Activities of Blend Essential Oil comprising Rose, Bergamot and Patchouli Oils

2018 ◽  
Vol 13 (5) ◽  
pp. 1934578X1801300
Author(s):  
Nuntapol Wongsukkasem ◽  
Orawan Soynark ◽  
Montira Suthakitmanus ◽  
Emprang Chongdiloet ◽  
Chidchanok Chairattanapituk ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Biological Activities ◽  
Volume Ratio ◽  
Sulphonic Acid ◽  
Tyrosinase Inhibition

Blended essential oil comprising rose, bergamot and patchouli (RBP) oils with the volume ratio of 6:3:1, respectively have been tested for various biological activities. The blended oil showed antimicrobial activity against Propionibacterium acnes and Staphylococcus epidermidis with minimum inhibitory concentration (MIC) of 0.003125 %v/v and 0.125 %v/v, respectively. For antioxidant, blended oil showed the half inhibitory concentration (IC50) at 0.67 %v/v and 0.14 %v/v while tested with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis(3-ethylbenzothiazoline-6 sulphonic acid) (ATBS) assays, respectively. Blended oil also has anti-tyrosinase activity with 45±4.6%tyrosinase inhibition at 4 mg/mL concentration using modified dopachrome method. However it has no activity on anti-collagenase and anti-elastase.

scholarly journals Effectiveness of Matoa Leaf (Pometia pinnata) Extract as an Antibacterial Staphylococcus epidermidis

2021 ◽  
Vol 3 (1) ◽  
pp. 1-8
Author(s):  
Rossalinda Rossalinda ◽  
Fitria Wijayanti ◽  
Damayanti Iskandar
Keyword(s):  
Staphylococcus Epidermidis ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Clear Zone ◽  
Disc Diffusion Method ◽  
Sample Concentration ◽  
Inhibition Zones

A natural alternative that can be used to inhibit the growth of Staphylococcus epidermidis bacteria is matoa leaves (Pometia pinnata). Matoa leaves contain secondary metabolites such as flavonoids, tanins, and saponins which are known as an antibacterial. The purpose of this study was to determine the effectiveness of matoa leaves extract as an antibacterial agent for Staphylococcus epidermidis. This study used the disc diffusion method, using deodorant as a positive control, aquadest as a negative control and concentration variations matoa leaves extract of 15%, 20%, 25% and 30%. The matoa leaves were extracted using the maceration method with 96% ethanol. Antibacterial testing in this study was characterized by a clear zone or zone of inhibition around the disc paper. The results of the antibacterial test in this study have to different inhibition zones, that is a sample concentration of 15% the inhibition zone value is 0,125 mm. The sample concentration of 20% the inhibition zone is 3 mm. The sample concentration of 25% the inhibition zone  is 2,312 mm and the sample concentrasion of 30% the inhibition zone is 0,875 mm. The extract of matoa leaves can be used as an antibacterial, because according to Pan Chen Wu Tang and zao (2009) the extract of matoa leaves could be applied as inhibitor of the growth of Staphylococcus epidermidis at a sample concentration of 20% is in the weak to moderate category

FORMULATION AND CHARACTERIZATION OINMENT OF ARUM MANIS MANGO (Mangifera indica L.) LEAVES METHANOL EXTRACTS AS AN ANTIBACTERIAL AGAINST Propionibacterium acnes

2020 ◽  
Vol 16 ◽  
Author(s):  
Dian Riana Ningsih ◽  
Zusfahair Zusfahair ◽  
Dadan Hermawan ◽  
Wulan Anggraeni ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Methanol Extract ◽  
Mangifera Indica ◽  
Inhibition Zone ◽  
Sweat Glands ◽  
Ointment Formulation ◽  
Inhibition Zones ◽  
Mango Leaves ◽  
Dispersive Power

Background: Acne is caused by several factors including the active secretion of sebaceous sweat glands, hyperkeratosis in the hair infundibulum and the effects of bacteria. One of the plants that has the potential as an antibacterial is the extract of arumanis mango leaves. Method: Determine the Minimum Inhibitory Concentration (MIC) of methanol extract of mango leaves which can inhibit Propionibacterium acnes activity. The antibacterial activity tests were performed using agar diffusion. The ointment formulation, the characteristics of ointment preparations and the ointment activity against P. Acnes are discussed. Result: MIC of methanol extract of mango leaves value is 5 ppm with an inhibition zone of 1 mm. The ointment obtained is white, has distinctive smell, semisolid form, possesses a pH of 4.92 - 5.87, dispersive power of 5.05 - 6.30 cm, adhesive power of 1 - 3.67 seconds, homogeneous and protective. Ointment preparations of methanol extract of mango leaves has activity on P. acnes on the 0 and 15th day of storage. The activities of ointment preparation on day 0 with concentrations of 0, 5, 10 and 15 ppm are 0.00 mm; 10.20 mm; 19.97 mm and 23.60 mm respectively, while the inhibition zones produced by the preparation of ointment on day 15 with concentrations of 0, 5, 10 and 15 ppm are 0.00 mm; 5.71 mm; 9.58 mm and 21.88 mm respectively. Conclusion: Methanol extract of mango leaves (Mangifera indica L.) and oinment preparation are able to inhibit the growth of Propionibacterium acnes.

scholarly journals STRUCTURE CHARACTERIZATION AND EVALUATION POTENTIAL OF ANTIMICROBIAL EXTRACTS FROM PHELLINUS LINTEUS AGAINST SKIN INFECTIOUS PATHOGENS, STAPHYLOCOCCUS EPIDERMIDIS ATCC12228 AND PROPIONIBACTERIUM ACNES DMST14916

2017 ◽  
Vol 9 (12) ◽  
pp. 78
Author(s):  
Surachai Techaoei ◽  
Pattaranut Eakwaropas ◽  
Khemjira Jarmkom ◽  
Warachate Khobjai
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Staphylococcus Epidermidis ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Structure Characterization ◽  
Diffusion Method ◽  
Phellinus Linteus ◽  
Chemical Profile

Objective: The objective of this study was to investigate the antimicrobial activity of Phellinus linteus against skin infectious pathogens, Staphylococcus epidermidis ATCC12228 and Propionibacterium acnes DMST 14916.Methods: Fungal fruiting bodies were extracted with 95% ethanol and ethyl acetate, and then, vaporized. The antimicrobial activities were determined by the disc diffusion method against Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228 skin infectious pathogens. A minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) for those crude extracts were determined. Finally, the chemical profile of crude extract was determined by using thin layer chromatography and GC-MS.Results: The result demonstrated that the ethanolic extraction had more active fractions with an MIC of 0.5 mg/ml against the growth of Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228 and also showed a minimum inhibitory concentration (MBC) at a concentration of 1.0 mg/ml, while ethyl acetate-based solvents failed to develop on TLC according to Retention factor (Rf) values of 0.71-0.76. The GC-MS was applied to investigate the chemical profile of crude extract of Phellinus linteus, revealing a component of hexadecanoic acid and 9, 12-octadecadienoic acid.Conclusion: Phellinus linteus fruiting body extracts have great potential as antimicrobial compounds against Propionibacterium acnes DMST 14916 and Staphylococcus epidermidis ATCC12228. Thus, they can be used in the treatment of infectious diseases caused by bacterial pathogens. 

scholarly journals Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts

2018 ◽  
Vol 66 (4) ◽  
pp. 1519
Author(s):  
Mailen Ortega Cuadros ◽  
Adriana Patricia Tofiño Rivera ◽  
Luciano Jose Merini ◽  
Maria Cecilia Martinez Pabon
Keyword(s):  
Antimicrobial Activity ◽  
Dental Caries ◽  
Essential Oil ◽  
Streptococcus Mutans ◽  
Human Fibroblasts ◽  
Positive Control ◽  
Negative Control ◽  
Cymbopogon Citratus ◽  
Significant Difference ◽  
Efficient Alternative

Dental caries is a pathology of multifactorial origin and currently natural products are an efficient alternative treatment; The work sought to evaluate the antimicrobial activity of the Cymbopogon citratus essential oil and the citral and myrcene components against Streptococcus mutans ATCC UA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. The viability effect against Streptococcus mutans on biofilms was evaluated through exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1, 0.1, and 0.01 µg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated on keratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control (negative control) and ethanol 1% as vehicle control (positive control). The three substances evaluated had effects on the viability of Streptococcus mutans with mortality between 74% and 96%, without significant difference among them (p > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24-h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studied further as potential co-adjuvants to prevent dental caries that cause minor adverse effects

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