scholarly journals Antimicrobial activity of Cymbopogon citratus (Poaceae) essential oil on Streptococcus mutans biofilm and cytotoxic effect on keratinocytes and fibroblasts

2018 ◽  
Vol 66 (4) ◽  
pp. 1519
Author(s):  
Mailen Ortega Cuadros ◽  
Adriana Patricia Tofiño Rivera ◽  
Luciano Jose Merini ◽  
Maria Cecilia Martinez Pabon
Keyword(s):  
Antimicrobial Activity ◽  
Dental Caries ◽  
Essential Oil ◽  
Streptococcus Mutans ◽  
Human Fibroblasts ◽  
Positive Control ◽  
Negative Control ◽  
Cymbopogon Citratus ◽  
Significant Difference ◽  
Efficient Alternative

Dental caries is a pathology of multifactorial origin and currently natural products are an efficient alternative treatment; The work sought to evaluate the antimicrobial activity of the Cymbopogon citratus essential oil and the citral and myrcene components against Streptococcus mutans ATCC UA159, as well as their cytotoxicity on keratinocytes and human fibroblasts. The viability effect against Streptococcus mutans on biofilms was evaluated through exposure to the three substances by using the MBEC technique-high-throughput at concentrations of 1, 0.1, and 0.01 µg/mL and chlorhexidine as positive control. The cytotoxicity of the compounds was evaluated on keratinocytes and fibroblasts through the MTT reduction technique, using 0.5 mM H2O2 as cell-death control (negative control) and ethanol 1% as vehicle control (positive control). The three substances evaluated had effects on the viability of Streptococcus mutans with mortality between 74% and 96%, without significant difference among them (p > 0.393); additionally, no cytotoxicity was evident on keratinocytes and fibroblasts in a 24-h treatment. The substances evaluated showed significant antimicrobial effects; hence, these should be studied further as potential co-adjuvants to prevent dental caries that cause minor adverse effects

scholarly journals Antimicrobial activity of the essential oil of Cymbopogon citratus (DC) Stapf. on Staphylococcus spp., Streptococcus mutans and Candida spp.

2013 ◽  
Vol 15 (4) ◽  
pp. 474-482 ◽  
Author(s):  
RBA Almeida ◽  
G Akisue ◽  
LML Cardoso ◽  
JC Junqueira ◽  
AO C Jorge
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Streptococcus Mutans ◽  
Control Group ◽  
Minimal Bactericidal Concentration ◽  
Cymbopogon Citratus ◽  
Candida Spp ◽  
Biofilm Growth ◽  
Micro Organisms ◽  
Staphylococcus Spp

Medicinal plants with fungicide action, antibacterial and anti-inflammatory effects are under investigation. The main purpose of this work was to evaluate the antimicrobial activity of the essential oil from Cymbopogon citratus (DC) Stapf. on strains of Staphylococcus spp., Streptococcus mutans and Candida spp. with planktonic and biofilm growth. To study the micro-organisms in planktonic cells, the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined by using 9 clinical strains for each species and 1 ATCC (American Type Culture Collection) from C. albicans, C. tropicalis, C. glabrata, S. aureus, S. epidermidis and S. mutans. In order to evaluate the effects of the essential oils on biofilms, strains of S. aureus (ATCC 6538), S. mutans (ATCC 35688) and C. albicans (ATCC 18804) were used. The biofilm was formed on acrylic resin discs with isolated micro-organisms or in associations. The number of colony-forming-units (CFU) obtained in each biofilm (CFU/ml) was submitted to Student's t statistical test. The results demonstrated that the essential oil of Cymbopogon citratus showed microbiostatic and microbicidal activity against all tested strains. The average CFU/ml for the biofilm of S. aureus, S. mutans and C. albicans, whether isolated or in association, was lower in the group treated with essential oil than in the control group.

scholarly journals Avaliação in vitro da Atividade Antimicrobiana de Extrato Alcoólico de Própolis Comparado à Solução de Clorexidina 0,12%

2017 ◽  
Vol 19 (2) ◽  
pp. 95
Author(s):  
Maria Paula Jacobucci Botelho ◽  
Amanda Da Silva ◽  
Fernanda Da Conceição Antônio Ferreira ◽  
Lígia Maria Molinari Capel
Keyword(s):  
Drinking Water ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Positive Control ◽  
Negative Control ◽  
Tooth Surface ◽  
Dental Biofilm ◽  
Fermentable Carbohydrates ◽  
Broad Understanding

Apesar dos esforços e do amplo entendimento sobre a cárie dental, está continua bastante prevalente em determinados segmentos da população. A doença apresenta etiologia multifatorial, com a dieta e os micro-organismos envolvidos desenvolvendo papéis fundamentais. Os Streptococcus mutans têm papel fundamental em seu início e é ubíquo em crianças a partir dos sete anos de idade. Para controlar essas bactérias é preciso uma dieta com baixo consumo de sacarose e de carboidratos fermentáveis, o que nem sempre se consegue. Sua remoção da superfície dentária só é possível por meio da fricção mecânica, mas pode-se prevenir sua presença no biofilme dentário através da utilização de antissépticos. O mais utilizado na Odontologia é a clorexidina, porém como não é isenta de efeitos adversos, atualmente, vêm-se estudando produtos alternativos, dentre os quais alguns agentes fitoterápicos. O objetivo deste trabalho foi analisar a atividade da própolis contra os S. mutans em relação à clorexidina. Foi analisada própolis 70% diluída em álcool 96% e após diluída em água potável 5ml, própolis 50%, própolis 12% e clorexidina 0,12% (controle positivo), e solução salina (controle negativo). O operador foi cego em relação às substâncias que estava utilizando. Somente a clorexidina 0.12% e a própolis 12% apresentaram halo de inibição claro. Neste estudo, a clorexidina apresentou melhor atividade contra os S.mutans.Palavras-chave: Clorexidina. Cárie Dentária. Streptococcus mutans.AbstractDespite the efforts and the broad understanding on dental caries, it is still quite prevalent in certain segments of the population. The disease shows a multifactorial etiology, with diet and microorganisms involved developing fundamental roles. The Streptococcus mutans has key role in its beginning and is ubiquitous in children as young as seven years of age. To control these bacteria a diet is necessary with low consumption of sucrose and of fermentable carbohydrates, which is not always possible. The tooth surface removal is only possible through mechanical friction, but it is possible to prevent the presence of dental biofilm through the use of antiseptics. The most widely used in dentistry is chlorhexidine, however as it is not devoid of adverse effects, currently alternative products have been studying, among which some herbal agents. The aim of this study was to analyze the activity of propolis against S. mutans compared to chlorhexidine. Propolis was analyzed diluted in 70% ethanol 96% and further diluted in drinking water 5ml, 50% propolis, propolis 12% and 0.12% chlorhexidine (positive control), and saline (negative control). The operator was blinded for the substances he was using. Only 0.12% chlorhexidine and 12% propolis showed clear inhibition halo. In this study the chlorhexidine presented better activity against S. mutans.Keywords: Chlorhexidine. Dental Caries. Antimicrobials. Streptococcus mutans

scholarly journals INHIBITION TEST OF METHANOL EXTRACT FROM SOURSOP LEAF (Annona muricata Linn.) AGAINST Streptococcus mutans BACTERIA*

2017 ◽  
Vol 17 (1) ◽  
pp. 23 ◽  
Author(s):  
Raudhatul Jannah ◽  
Muhammad Ali Husni ◽  
Risa Nursanty
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Methanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Annona Muricata ◽  
High Prevalence ◽  
Chemical Content ◽  
And Mathematics

Abstract. Dental caries is a disease with a high prevalence of caries in Indonesia is caused by the bacterium Streptococcus mutans. The leaves of the soursop (Annona muricata Linn.) is one of the herbs that can remove dental plaque. This study aims to determine the chemical content and measure the inhibitory antibacterial soursop leaft methanol extractin inhibiting the growth of Streptococcus mutans bacteria. Phytochemical screening stated that the leaf of the soursop contains flavonoids, alkaloids, tannins, steroids, glycosides and saponins. Test of inhibition were measured using paper disc diffusion method with variation concentrations of 5, 10, 15 20, and 25%, positive control is tetrasilkin 30 µg, and a negative control is methanol. Antibacterial test results showed that the methanol extract of soursop leaves have antibacterial activity against Streptococcus mutans at concentrations of 5, 10, 15, 20 and 25% with inhibitory diameter of 9,1; 10,57; 11,53; 12,01 and 13,75 mm respectively. Keywords: Soursop leaves, Dental caries, Streptococcus mutans, Methanol.*Judul ini telah dipresentasikan pada Seminar Nasional: Indonesian Students Conference on Science and Mathematics (ISCSM) 11-12 November 2015, Banda Aceh Indonesia

scholarly journals Comparison of Antimicrobial Activity between Chemical Disinfectants on Contaminated Orthodontic Pliers

2015 ◽  
Vol 16 (8) ◽  
pp. 619-623 ◽  
Author(s):  
Mateus Rodrigues Tonetto ◽  
Marcos André dos Santos da Silva ◽  
Milton Carlos Kuga ◽  
Matheus Coelho Bandeca ◽  
Célia Regina Maio Pinzan-Vercelino ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Peracetic Acid ◽  
Isopropyl Alcohol ◽  
Positive Control ◽  
Negative Control ◽  
Immersion Method ◽  
Chemical Substances ◽  
Significant Difference ◽  
Group 5

ABSTRACT Aim To compare the antimicrobial activity of the chemical substances—70% isopropyl alcohol, 2% glutaraldehyde (GTA) and 0.25% peracetic acid (PAA) in disinfecting orthodontic pliers contaminated in vitro with Streptococcus mutans, Staphylococcus aureus and Candida albicans. Materials and methods Distal end cutter pliers were divided into five groups: group 1 (negative control—sterilized pliers), group 2 (positive control—sterilized plier, subsequently contaminated), group 3 (disinfected with 70% isopropyl alcohol, friction method), group 4 (disinfected with 2% GTA, immersion method for 30 minutes), group 5 (disinfected with 0.25% peracetic acid (PAA), immersion method for 10 minutes). After the pliers were treated with one disinfectant and submitted to microbiological evaluation (by counting colony forming units), they were submitted to the same cleansing, sterilizing and contaminating processes, and were used in the following groups (crossover and washout study). The two-factor analysis of variance (ANOVA) test, followed by the Tukey test, was used to compare the groups. Results The results showed that there was no statistically significant difference between the three tested disinfectants. Conclusion Although there were no statistically significant differences between the disinfectants, the chemical agents 2% glutaraldehyde and 0.25% PAA were effective in inhibiting the growth of the three microorganisms tested; however, 70% isopropyl alcohol was unable to completely eliminate S. aureus. Clinical significance The chemical substances 2% glutaraldehyde and 0.25% PAA completely eliminated the microorganisms tested. How to cite this article Carvalho MRA, dos Santos da Silva MA, de Sousa Brito CAR, Campelo V, Kuga MC, Tonetto MR, De Jesus Tavarez RR, Bandéca MC, Pinzan-Vercelino CRM. Comparison of Antimicrobial Activity between Chemical Disinfectants on Contaminated Orthodontic Pliers. J Contemp Dent Pract 2015;16(8):619-623.

The Effectiveness Of Anchovy Concentration (Stolephorus insularis) as Antimicrobial to Streptococcus mutans (In Vitro)

DENTA ◽  
2016 ◽  
Vol 10 (1) ◽  
pp. 71
Author(s):  
Almira Fa’Izah ◽  
Istien Wardani ◽  
Diana Soesilo
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Control Group ◽  
Oral Diseases ◽  
Clear Zone ◽  
Control Group Design

<p><strong><em>Background:</em></strong><em> Dental and oral diseases which </em><em>a</em><em>re often found in children is dental caries. Streptococcus mutans </em><em>i</em><em>s the main cause of caries. Caries c</em><em>an</em><em> be prevented by using a topical application of fluoride. The Anchovy (Stolephorus insularis) contain</em><em>s</em><em> protein, vitamins (A, B<sub>1</sub>, C), and minerals (Fe, Ca, K, F). Calcium fluoride (CaF<sub>2</sub>) within the anchovy c</em><em>an</em><em> inhibit the occurrence of dental caries. <strong>Purpose</strong></em><strong><em>: </em></strong><em>The aim of this study was </em><em>to </em><em>determine the </em><em>antimicrobial </em><em> ability of anchovy extract (Stolephorus insularis) to Streptococcus mutans. <strong>Materials and</strong> <strong>Methods: </strong>This study was a laboratory experimental research </em><em>with</em><em>  post test only control group design. Diffusion method were applied with 2 controls: negative control used DMSO 1%, positive control used NaF solution, and 3 concentrations of anchovy extract (Stolephorus insularis) 3%, 6%, and 12%, each group were composed of 6 samples. </em><em>Antimicrobial</em><em> was assessed by measuring the diameter of the clear zone around the discs contained the anchovy extract (Stolephorus insularis). Data were analyzed by Kruskal-Wallis test followed by Mann-Whitney test.<strong> Result:</strong> The results from this study showed clear zone around the discs of the anchovy extract (Stolephorus insularis). The more concentration of the extract showed the more </em><em>antimirobial</em><em> zone diameter. The average zone of </em><em>antimicrobial</em><em> at  </em><em>the </em><em>concentration of  3% </em><em>were </em><em>7,11 mm, 6%</em><em> </em><em>9,5 mm, 12%</em><em> </em><em>10,78 mm, for the negative control DMSO 1% 6 mm and the positive control NaF solution 8,16 mm. The largest diameter of the clear zone was at concentration of 12% (P &lt; 0,05).</em><em> </em><strong><em>Conclusion: </em></strong><em>The<strong> </strong>anchovy extract (Stolephorus insularis) had an antimicrobial effect to the growth of Streptococcus mutans.</em><em></em></p>

scholarly journals Ethanol extract of soursop leaves inhibits acid production and adhesion of Streptococcus mutans

2021 ◽  
Vol 33 (3) ◽  
pp. 279
Author(s):  
Friska Ani Rahman ◽  
Rahsunji Intan
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Acid Production ◽  
Ph Value ◽  
Herbal Remedy ◽  
Ethanol Extract ◽  
Negative Control ◽  
Annona Muricata ◽  
Cariogenic Bacteria ◽  
Significant Difference

ABSTRACTIntroduction: Dental caries and dental plaque are the most popular global oral health problems. The primary step of dental caries is characterized by damage of tooth surfaces  affected by acids which are by-products of sugar metabolism by a cariogenic bacteria. One of the cariogenic bacteria is Streptococcus mutans. Annona muricata is traditionally applied as an herbal remedy for various illnesses and has been recognized in a previous study as an antimicrobial agent. The aim of this study was to investigate  acid production and adhesion of ethanol extract of Soursop on Streptococcus mutans. Methods: Soursop leaf extracted by maceration using 70% ethanol solvent. The extracts obtained were tested at various concentrations. To examine the effect of ethanol extract of Soursop on acid production by S. mutans, the pH of the culture was determined using a pH meter. Inhibition of adhesion of S. mutans to the saliva-coated hydroxyapatite (S-HA) discs was quantified using colony counting on TYS20B agar plates. Results: The pH of S. mutans cultures in the presence of ethanol extract of Soursop leaf at various concentrations was higher than negative control, but there were no differences in pH value between the various concentrations of ethanol extract of Soursop leaf. Adhesion of S. mutans to S-HA discs was inhibited by various concentrations of ethanol extract of Soursop leaf. Adhesion decreased with increasing concentrations of ethanol extract of Soursop leaf, but there was not significant difference in colony count between the various concentrations of ethanol extract of Soursop leaf. Conclusions: Ethanol extract of Soursop leaf attenuates the acid production and adhesion of S. mutans to hydroxyapatite discs. Keywords: Soursop (Annona muricata); Streptococcus mutans; acid production; adhesion

scholarly journals EFEKTIFITAS ANTIBAKTERI EKSTRAK BUAH NAGA SUPER MERAH (Hylocereus costaricensis) TERHADAP PERTUMBUHAN BAKTERI Streptococcus mutans

2018 ◽  
Vol 11 (2) ◽  
pp. 52
Author(s):  
Bambang Tri Hartomo ◽  
Fanny Kusuma Djati ◽  
Fitri Diah Oktadewi ◽  
Angger Waspodo Dias Andrianto ◽  
Prasetyo Adi Nugroho
Keyword(s):  
Streptococcus Mutans ◽  
Positive Control ◽  
Negative Control ◽  
Fruit Extract ◽  
Bacterial Agent ◽  
Fruit Skin ◽  
Dragon Fruit ◽  
Significant Difference ◽  
Inhibitory Power

Buah naga super merah (Hylocereus costaricencis)saat ini dibudidayakan oleh Kebun Benih Holtikultura Baturaden sebagai produk unggulan yang berpotensi meningkatkan kesejahteraan masyarakat Banyumas. Buah naga super merah diyakini memiliki efek anti bakteri oleh karena kandungan polifenol yang terdapat pada daging buah maupun kulit buah naga. Penelitian ini bertujuan untuk melihat daya hambat ekstrak buah naga super merah terhadap pertumbuhan bakteri Streptococcus mutans yang merupakan salah satu pencetus terjadinya penyakit periodontal. Sebagai langkah awal, dilakukan uji determinasi buah naga super merah untuk memastikan bahwa buah naga yang digunakan adalah buah naga super merah. Selanjutnya dilakukan pembuatan ekstrak dengan metode maserasi. Hasil penelitian antar perlakuan menunjukan bahwa tidak terdapat perbedaan daya hambat bakteri yang signifikan (p>0,05) antara ekstrak buah naga super merah, kontrol positif dan kontrol negatif. Penelitian ini menyimpulkan bahwa ekstrak buah naga super merah dapat digunakan sebagai bahan antibakteri dalam terapi periodontal. Hasil penelitian ini diharapkan akan menjadi acuan untuk langkah berikutnya yaitu pengujian efek anti bakteri larutan buah naga super merah terhadap akumulasi plak pada rongga mulut.   Super red dragon fruit (Hylocereus costaricencis) is currently cultivated by the Baturraden Horticultural Seed Garden as a superior product that has the potential to improve the welfare of the Banyumas community. Dragon fruit is believed to have anti-bacterial effects because of the polyphenol content found in fruit flesh and dragon fruit skin. This study aims to see the inhibitory power of super red dragon fruit extract on the growth of Streptococcus mutans bacteria which is one of the triggers of periodontal disease. As a first step, a determination of dragon fruit is determined to ensure that the dragon fruit used is super red dragon fruit. Furthermore, the extract was made by maceration method. The results of the study between treatments showed that there was no significant difference in bacterial inhibition (p> 0.05) between super red dragon fruit extract, positive control and negative control. This study concluded that super red dragon fruit extract can be used as anti bacterial agent for periodontal therapy. The results of this study are expected to be a reference for the next research, analyzing the antibacterial effect of super red dragon fruit solution to the accumulation of plaque on the oral cavity

scholarly journals Perbedaan Kekasaran Permukaan Resin Akrilik Heat Cured Setelah Penyikatan Dengan Ekstrak Daun Sereh Konsentrasi 30% Dan 60% Dalam Pasta Gigi

DENTA ◽  
2019 ◽  
Vol 13 (1) ◽  
Author(s):  
Paulus Budi Teguh ◽  
Linda Rochyani ◽  
Clarissa Valerie Wardojo
Keyword(s):  
Surface Roughness ◽  
Acrylic Resin ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Cymbopogon Citratus ◽  
Significant Difference ◽  
Maximum Limit

<p><strong><em>Background: </em></strong><em>Tooth loss can be replaced by the use of dentures. </em><em>Cleaning of dentures by brushing using toothpaste can cause surface roughness because</em><em> of</em><em> abrasive materials. The use of toothpaste can be replaced with </em><em>paste of extract </em><em>Cymbopogon citratus because it has antibacterial and antifungal </em><em>effects</em><em>.</em><em> </em><strong><em>Purpose:</em></strong><em> </em><em>to determine the effect of brushing using </em><em>paste of extract Cymbopogon citratus </em><em>on the surface</em><em> roughness</em><em> of heat cured </em><em>acrylic </em><em>resin. <strong>Methods:</strong> </em><em>S</em><em>ample w</em><em>ere</em><em> heat cured acrylic resin (62.5 x 10 x 2.5) mm, divided into 4 groups</em><em> consist of negative control group was brushed with basic paste, positive control group was brushed with herb paste, and two groups were brushed with paste of Cymbopogon citratus extract with 30% and 60% concentrations. Brushing was done for 3 minutes with 60 brushing times and then measured with surface roughness tester. The data was processed by ANOVA test. <strong>Result: </strong></em><em>The average surface roughness in K-, K</em><em>+</em><em>, P1, and P2 </em><em>were</em><em> 0</em><em>,</em><em>1217, 0</em><em>,</em><em>3433, 0</em><em>,</em><em>1633, and 0</em><em>,</em><em>27 μm. There was significant difference between negative control group and positive control group; negative control group with P1 and P2</em><em> group</em><em>; positive control group with  P1 and P2</em><em> group</em><em>; P1</em><em> group</em><em> with P2 group (P &lt;0.05).</em><em> </em><strong><em>Conclusion: </em></strong><em>The paste of Cymbopogon citratus extract concentration of 30% was</em><em> effectively used because the level of surface roughness below the maximum limit of recommended surface roughness of dentistry material</em><em> which</em><em> is 0</em><em>,</em><em>2 μm.</em><em> The 60% concentration paste of Cymbopogon citratus extract was less effective </em><em>because the level of surface roughness above the maximum limit of surface roughness is recommended.</em><em></em></p>

scholarly journals The inhibition of Streptococcus mutans glucosyltransferase enzyme activity by mangosteen pericarp extract

2017 ◽  
Vol 50 (2) ◽  
pp. 97 ◽  
Author(s):  
Nirawati Pribadi ◽  
Yovita Yonas ◽  
Widya Saraswati
Keyword(s):  
Enzyme Activity ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
High Performance ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Inhibitory Power ◽  
Glucan Synthesis

Background: Streptococcus mutans (S. mutans) is a bacterium that plays an important role in the pathogenesis of dental caries. Streptococcus mutans produces the glucosyltransferase enzyme which is capable of catalyzing glucan synthesis in the progression of dental caries. Certain treatments involving traditional plant use have been developed to eradicate Streptococcus mutans as a means of preventing the formation of dental caries. One of these is mangosteen pericarp extract containing a number of polyphenols that have the capacity to act as antibacterial agents, namely; tannin, mangostin, and flavonoid. Purpose: The research aimed to investigate the inhibitory power of mangosteen pericarp extract against Streptococcus mutans producing the glucosyltransferase enzyme. Methods: The research used mangosteen pericarp extract at concentrations of 0.39% and 0.78% as the treatments, while 0.12% chlorhexidine gluconate was used as a positive control, and distilled water as a negative control. Each group consisted of six samples. Mangosteen peels extracted with 96% ethanol (maceration method) and mangosteen extract constituted 5% of the total weight of the mangosteen pericarp. Supernatant containing Gtf enzyme produced from a culture medium and centrifuged at 1500 rpm for 10 minutes at 4o C. Glucosyltransferase enzyme activity was measured by analyzing the extensive fructose area by means of High Performance Liquid Chromatography (HPLC). The extensive fructose area was determined according to time retention in each group. Results: Mangosteen peel extract at concentrations of 0.39% and 0.78% demonstrated greater ability than the negative control group (sterile aquades) and similar ability to the positive group (chlorhexidine 0.12%) to inhibit the activity of the Gtf enzyme or S. mutans bacteria. Conclusion: Mangosteen pericarp extract has the ability to inhibit the activity of Streptococcus mutans in producing glucosyltransferase enzyme.

scholarly journals Actividad antifúngica de Cymbopogon citratus contra Colletotrichum gloeosporioides

2017 ◽  
Vol 28 (2) ◽  
pp. 465 ◽  
Author(s):  
Alexander Francisco Pérez Cordero ◽  
Leonardo Miguel Chamorro Anaya ◽  
Deimer Calixto Vitola Romero ◽  
Jesús María Hernández Gómez
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Activity ◽  
Colletotrichum Gloeosporioides ◽  
Chemical Characterization ◽  
Positive Control ◽  
Negative Control ◽  
Cymbopogon Citratus ◽  
A Value

The objective of this research was to evaluate in vitro the inhibitory activity of essential oils from fresh leaves of Cymbopogon citratus (lemongrass) against Colletotrichum gloeosporioides in yam. The research was conducted in the department of Sucre, Colombia. The essential oils of C. citratus were collected in the municipalities of Sincelejo, La Union and Sampues, in September and October 2015. The essential oil was extract from fresh leaves using the microwave-assisted hydrodistillation. Concentrations of 5000, 8000 and 10 000 ppm of each essential oil were prepared. An absolute control, a positive control (benomyl 1 g/l) and a negative control was used. An inhibitory activity was obtained by using the direct seeding on surface of the potato-dextrose-agar method and it was expressed as percentage of inhibition rate. The chemical characterization of essential oils was performed by gas chromatography coupled to mass spectrometry. The highest percentages of antifungal index were observed in the lemongrass from Sincelejo at concentrations of 5000, 8000 and 10 000 ppm, after in La Union at 8000 and 10 000 ppm, and finally at Sampues at 10 000 ppm, with a value of 97.77%; the effect was similar to the positive control with benomyl 1 g/l. Citral was the main constituent of the essential oils extracted. The essential oils obtained from the three municipalities showed antifungal activity against C. gloeosporioides. 

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