scholarly journals Effectiveness of Beauveria bassiana Powder as Larvacide to Aedes aegypti Linnaeus, 1762 Larvae

2018 ◽  
Vol 19 (1) ◽  
pp. 45
Author(s):  
Nova Hariani ◽  
Eka Wulandari ◽  
Bodhi Dharma
Keyword(s):  
Experimental Design ◽  
Aedes Aegypti ◽  
Beauveria Bassiana ◽  
Natural Sciences ◽  
Product Concentration ◽  
Percentage Mortality ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Mortality Percentage ◽  
Third Instar Larvae

Study of effectiveness of Beauveria bassiana Powder as Larvacide to Aedes aegypti larvae was conducted on September 2016 to May 2017 in Ecologi and Systematics Animal Laboratory, Faculty of Mathematics and Natural Sciences, University of Mulawarman. This study aimed to determine the effect of Beauveria bassiana powder with use flour rice media on differents concentration as larvacide to mortality of larvae Aedes aegypti. Stages of study include arranged experimental design (Completely Randomized Design) with concentration of 0%, 0.125%, 0.25%. 0.5% and 0.75%, taking sample, rearing third instar larvae of Aedes aegypti and application Beauveria bassiana powder in 48 hours observation. The mortality value of larvae willbe presented on percentage tabel and graph. The result showed, this product is capable of killing larvae for 48 hours with the percentage mortality 0%, 10%, 28%, 70% and 80% in each concentration. Mortality of larvae is influenced by concentration and period of exposure. Increased of product concentration that given and period of exposure can effected increased of mortality percentage. Keywords: mortality, Beauveria bassiana powder, Aedes aegypti larvae

scholarly journals Analisis Efektifitas Tepung Jamur sebagai Larvasida Aedes aegypti

2012 ◽  
Vol 14 (1) ◽  
pp. 126
Author(s):  
Yekki Yasmin ◽  
Lenni Fitri ◽  
Betty Mauliya Bustam
Keyword(s):  
Aedes Aegypti ◽  
Beauveria Bassiana ◽  
Storage Time ◽  
Natural Sciences ◽  
The Other ◽  
Biology Department ◽  
Randomized Design ◽  
Two Factors ◽  
Result Show ◽  
Completely Randomized Design

This research is about effectivity analysis of two kinds of fungi i.e. Beauveria bassiana and Metharrizium anisopliae as Aedes aegyptilarvacide, it has been conducted at laboratories Zoology in Biology Department, Mathematics and Natural Sciences Faculty, Syiah KualaUniversity. Aims of this research were estimating maximum time storage of fungi powder as effective larvacide and the number of bacteriacolonies were found in water used for experiment. This research was used Completely Randomized Design (CRD). The experiments consistof two factors i.e kind and saving time of fungi. The result show that the longer the storage time of Beauveria bassiana the more ineffectiveit was as larvacide and the least fungi colonies were found. Metharrizium anisopliae on the other hand, the longer it was kept, the moreeffective it was as larvacide and the more fungi colonies were found.

scholarly journals EFEKTIVITAS INSEKTISIDA NABATI DAUN SALAM (SYZYGIUM POLYANTHUM) TERHADAP MORTALITAS NYAMUK AEDES AEGYPTI

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Ana Windari ◽  
Mimatun Nasihah ◽  
Nur Lathifah Syakbanah
Keyword(s):  
Aedes Aegypti ◽  
Hemorrhagic Fever ◽  
Negative Effects ◽  
Randomized Design ◽  
Mosquito Mortality ◽  
Quasi Experimental ◽  
Completely Randomized Design ◽  
The Right ◽  
Mortality Percentage ◽  
Leaf Vegetable

Dengue Hemorrhagic Fever (DHF) is an acute disease in the form of dengue virus infection which is spread through mosquito bites. The use of synthetic insecticides actually causes negative effects on the environment, so another alternative is needed, namely the use of bay leaf vegetable insecticides. The purpose of this study was to determine the effectiveness of bay leaf insecticide (Syzygium polyanthum) as an insecticide against the mortality of Aedes Aegypti mosquitoes with the liquid electric method. This study used a quasi-experimental (quasi-experimental) design with a completely randomized design (CRD) method where the experiment was conducted on 150 mosquitoes in 5 treatments and 3 replications. The results of the percentage of bay leaf solution (Syzgium polyanthum) which has the largest number of mosquito mortality at a concentration of 80 ml/200 ml of water with a mortality percentage of 60%. The result of probit LC50 analysis of bay leaf solution was 64,315 g/ml. The higher the concentration of the solution given, the greater the number of mosquito mortality. It is necessary to modify the right tools and methods to make a solution of bay leaf (Syzgium polyanthum) so that it is more effectively applied in the community.Keywords: bioinsecticide, bay leaf, liquid electric, mortality, Aedes aegypti

scholarly journals Larvicidal Effect of Mixture of Beauveria bassiana Crude Metabolite and Chitinase Enzyme against Aedes aegypti Larvae

2018 ◽  
Vol 12 (4) ◽  
pp. 178
Author(s):  
Dyah Widiastuti ◽  
Bina Ikawati ◽  
Upik Kesumawati Hadi
Keyword(s):  
Aedes Aegypti ◽  
Beauveria Bassiana ◽  
Larval Mortality ◽  
Probit Analysis ◽  
Chemical Insecticide ◽  
Chitinolytic Bacteria ◽  
Larvicidal Effect ◽  
Randomized Design ◽  
Development Unit ◽  
Completely Randomized Design

Aedes aegypti is a major vector of dengue, a deadly disease causing deaths of millions of people in developing countries, both in urban and rural populations. Aedes aegypti control with chemical insecticide is widely used, and affects on a widespread insecticide resistance. Mosquito biological control was needed to replace the use of chemical insecticide. This study aimed to evaluate larvicidal effect of mixture of Beauveria bassiana crude metabolite and chitinase enzyme against Aedes aegypti larvae. An experimental study using completely Randomized Design was conducted in March-April 2016 at Banjarnegara Research and Development Unit for Zoonosis Control. Biolarvacide formula was made of mixture with 2 : 1 ratio between Beauveria bassiana crude metabolite and chitinase isolated from chitinolytic bacteria, which was propagated by the Agency’s Bacteriology Laboratory. In experimental method, 120 Aedes aegyptilarvae (3rd instar) were exposed with four concentrations of biolarvacide formula (4%, 8%, 16%, and 32%) in 3 replicates. Results showed that exposure of biolarvacide formula caused the third instar larval mortality which started on the first day of exposure. Probit analysis showed LC50 value was obtained at concentration of 53.2 ppm. This shows that larvacide formula of Beauveria bassiana and chitinase enzyme compounds are effective to be used as larvacides against Aedes aegypti larvae.

Pengaruh ovaprim, aromatase inhibitor, dan hipofisa terhadap kualitas telur ikan Lele Dumbo (Clarias gariepinus)

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Erwin A. Aziz ◽  
Ockstan Kalesaran
Keyword(s):  
Survival Rate ◽  
Experimental Design ◽  
Aromatase Inhibitor ◽  
Fish Larvae ◽  
Clarias Gariepinus ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
The Difference ◽  
Hatching Eggs

This study aimed to determine the effect of ovaprim hormone, aromatase inhibitor and pituitary on the quality of the catfish eggs (Clarias gariepinus). Experimental Design used was Completely Randomized Design (CRD) with four treatments, each with three replications. Treatment A: ovaprim; treatment B: Aromatase inhibitors, treatment C: hypophysis and treatment D: Control. The results showed that the difference in treatment gave highly significant effect on fertilization and hatching eggs but no significant effect on the survival rate of larvae. Aromatase inhibitor hormone was the best because it provided highly significant effect on fertilization (92.66%), hatchability of eggs (95%), and surviva rate (81.33%) of fish larvae.   Keywords : Clarias gariepinus. Ovaprim, Aromatase Inhibitor, Hypophysis, egg, larvae

scholarly journals PENGARUH NAUNGAN TERHADAP PERTUMBUHAN SEMAI MAKILA (Litsea angulata)

2017 ◽  
Vol 1 (3) ◽  
pp. 262
Author(s):  
Edom Bayau
Keyword(s):  
Experimental Design ◽  
Rheumatic Diseases ◽  
Seedling Growth ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Good For

   Makila (Litsea angulata) is a type of commercial timber and is very attractive to many people because of good quality and can also be used as a medicine in curing rheumatic diseases should be assigned the paramount concern. cultivation / reproduce makila can be done in a fast way is through the nursery in the field. in nurseries should also pay attention to things that affect makila, one of which is related to the intensity of sunlight makila seedling growth. The research conducted at the location of the greenhouse Faculty of Agriculture, University of Pattimura which take place september until november 2014 with the aim to determine the seedling growth makila (Litsea angulata) to determine what percentage of shade is good for seedling growth makila (Litsea angulata). The experimental design used was completely randomized design (CRD) with 5 (five) treatments, the seedlings without using Shade, Shade 30 percent, 50 percent Shade, Shade 65 percent, and Shade 75 percent. The results showed that the seedling Makila (Litsea angulata) is a kind of tolerance with all shade or kind makila able to grow in places that are open and shaded place.

scholarly journals EFEKTIFITAS CAMPURAN RENDAMAN JERAMI (ORYZA SATIVA L) DAN TEMEFOS SEBAGAI ATRAKTAN TERHADAP LETHAL OVITRAP AEDES AEGYPTI L

2020 ◽  
Vol 19 (2) ◽  
pp. 112-118
Author(s):  
Muhammad Rasyid Ridha ◽  
Budi Hairani ◽  
Gusti Meliyanie ◽  
Wulan Rasna Giri Sembiring ◽  
Abdullah Fadilly ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Hemorrhagic Fever ◽  
Control Strategy ◽  
Oryza Sativa L ◽  
Larval Mortality ◽  
Hemorrhagic Fever ◽  
Alternative Control ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Global Health Problem

ABSTRACT Dengue hemorrhagic fever is a global health problem and can be transmitted through vectors, namely Aedes aegypti. One of the controls can be through lethal ovitrap combined with attractant. This study aims to compare the percentage of trapped eggs, the number of eggs hatched, and larval mortality in the lethal ovitrap that were given two additional types of attractants. The insecticides used were temephos, while the attractants used were 20% straw soaking water, and water that Ae. aegypti have used to lay eggs. The type of research is an experiment with a completely randomized design. The sample used was female mosquito of Ae. aegypti that is full of blood from laboratory colonization. The results showed that straw soaking water was more influential attractant than the former Ae. aegypti colonization water in attracting Ae. aegypti mosquitoes to lay eggs. The highest larval mortality was found in a combination of lethal ovitrap with straw soaking water. Statistically there is an influence of the type of attractant on hatchability and the development of Ae. aegypti into adult mosquitoes. The combination of lethal ovitrap and attractant of straw soaking water can be an alternative control strategy for DHF program managers to reduce the density of Ae. aegypti mosquitoes and minimize transmission of dengue hemorrhagic fever in an area. Keywords: Aedes aegypti, attractant, lethal ovitrap, straw soaking water   ABSTRAK Demam berdarah dengue merupakan masalah kesehatan global dan dapat ditularkan melalui vektor yaitu Aedes aegypti. Salah satu pengendaliannya dapat melalui lethal ovitrap yang dipadukan dengan atraktan. Penelitian ini bertujuan untuk membandingkan persentase jumlah telur terperangkap, jumlah telur menetas, dan mortalitas larva pada lethal ovitrap yang diberi tambahan dua jenis atraktan. Insektisida yang digunakan adalah temefos, sedangkan atraktan yang digunakan adalah air rendaman jerami dengan konsentrasi 20%, dan air bekas kolonisasi/telur larva Ae. aegypti. Jenis penelitian ini adalah eksperimen dengan rancangan acak lengkap. Sampel yang digunakan adalah nyamuk Ae. aegypti betina yang kenyang darah hasil kolonisasi di laboratorium. Hasil penelitian menunjukkan bahwa air rendaman jerami merupakan atraktan yang lebih berpengaruh dibandingkan air bekas kolonisasi Ae. aegypti dalam menarik nyamuk Ae. aegypti untuk bertelur. Mortalitas larva tertinggi terdapat pada kombinasi lethal ovitrap dengan air rendaman jerami. Secara statistik ada pengaruh jenis atraktan terhadap daya tetas dan perkembangan Ae. aegypti menjadi nyamuk dewasa. Kombinasi lethal ovitrap dengan atraktan air rendaman jerami dapat menjadi strategi pengendalian alternatif bagi pengelola program DBD untuk mengurangi kepadatan nyamuk Ae. aegypti dan meminimalisasi transmisi penyakit demam berdarah dengue di suatu wilayah. Kata kunci: Aedes aegypti, atraktan, lethal ovitrap, rendaman jerami

scholarly journals Effect of Storage Temperature on Beauveria bassiana (Bals.) Vuill. Viability on Several Carriers

2006 ◽  
Vol 22 (1) ◽  
Author(s):  
Sri Sukamto
Keyword(s):  
Beauveria Bassiana ◽  
Storage Temperature ◽  
Ultra Violet ◽  
Rice Flour ◽  
Plant Diseases ◽  
Maize Flour ◽  
The Third ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tapioca Flour

One of the entomopatogenous fungus types commonly observed and showed potency as myco-insectiside is Beauveria bassiana(Bals.) Vuill.In order to support effectiveness and patogenous activity of B. bassiana, it is necessary to add a carrying agent that protects its spores from ultra violet ray. This study aims to investigate the effect of storage temperature on viability of B. Bassianaspores on the carrier material. The observation was carried out in the Laboratory of Plant Diseases, Indonesian Coffee and Cocoa Research Institute. The research was arranged in completely randomized design by three factors. The first factor was carrier (C), that consists of C1 = rice flour, C2 = maize flour and C3 = tapioca flour. The second factor was dosage (D), that consists of D1 = 1 g B. bassiana+ 1 g carrier; D2 = 1 g B. bassiana+ 5 g carrier and D3 = 1 g B. bassiana+ 10 g carrier. The third factor was temperature of the storage (T),that consists of T1 = 5oC; T2 = 23oC and T3 = 29oC. Viability of B. Bassiana spores was examined by observing development of 100 blastopores randomly and determined under light microscope with 400 times magnification. Observation was conducted in two replicates after the spores of B. bassiana were kept in the storage for 2, 4, 8 and 16 weeks. The result showed that by adding 1 g tapioca flour and temperature of storage of 5oC was potentiall in keeping viability of B. bassianaspores at least for 2 months. It was due to that tapioca flour gave better effect than rice and maize flours in keeping the storage and appropriate low temperature. Viability of B. bassianaspores decreased with increasing carrier dosage, temperature and duration of the storage. Whereas, storage at 5oC was found to be a better condition in keeping viability of dry pure B. bassianaspores longer than conditions of 23o and 29oC. Key word:Beauveria bassiana, temperature, viability,carrier.

scholarly journals Pemanfaatan sukun muda untuk peningkatan nilai gizi abon ikan tongkol

2021 ◽  
Vol 2 (2) ◽  
pp. 185
Author(s):  
Eva Fitriyaningsih
Keyword(s):  
Experimental Design ◽  
Nutritional Value ◽  
Economic Value ◽  
Chemical Properties ◽  
Animal Nutrition ◽  
P Value ◽  
Randomized Design ◽  
Study Results ◽  
Completely Randomized Design ◽  
Actual Participation

Background: Using young breadfruit to increase the breadfruit usability and economic value, one of the efforts is through the enrichment of the type of processed breadfruit products, breadfruit provides nutritional value that is beneficial to health, one of the help of breadfruit is increasing breadfruit for shredded products.Objectives: This study aims to find out how to improve young breadfruit 20%, 30%, 40% of the chemical properties (carbohydrate, protein, and fiber) of shredded tuna.Methods: This study used an experimental design with a non-factorial Completely Randomized Design (CRD) with three preparations and three repetitions. Then the chemical tests (carbohydrate, protein, and fiber) at the Laboratory of Animal Nutrition and Feed Technology of the Faculty of Agriculture, Syiah Kuala University, Banda Aceh.Results: The study results showed actual participation in young breadfruit 20%, 30%, and 40% of the chemical properties (carbohydrate, protein, and fiber) in shredded tuna with a p-value < 0.05.Conclusion: Agreeing that 20%, 30%, and 40% of young breadfruit were approved for shredded tuna's chemical properties (carbohydrate, protein, and fiber).

scholarly journals PENGARUH WAKTU MASERASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN PEGAGAN (Centella asiatica (L.) Urban)

2020 ◽  
Vol 9 (4) ◽  
pp. 482
Author(s):  
Ni Komang Trisna Rahayu ◽  
I Dewa Gede Mayun Permana ◽  
GA. Kadek Diah Puspawati
Keyword(s):  
Antioxidant Activity ◽  
Experimental Design ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Total Flavonoid ◽  
Time Duration ◽  
Time Treatment ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Effect On Yield

This research aimed to determine the effect of maceration time on the antioxidant activity of pegagan leaf extract and to determine the best maceration time with highest antioxidant activity of pegagan leaf extract. The experimental design used was Completely Randomized Design with maceration time duration there were 18, 24, 30, 36, 42, and 48 hours. All of the treatment repeated three times to obtained 18 units of experiments. The data analyzed by analyze of variance, if the treatment had a significant effect followed by The Duncan Test. The results showed that the maceration time treatment had a significant effect on yield, total phenolik, total flavonoid, total tannin and antioxidant activity of pegagan leaf extract. The 24 hour maceration time was the best treatment with antioxidant activity 66.67%, IC50 632.82 ppm, a yield of 24.30%, a total phenolik of 57.85 mgGAE / g, a total flavonoid of 105.28 mgQE / g. total tannin 54.09 mgTAE / g.

scholarly journals PENAMBAHAN BEBERAPA JENIS BAHAN NUTRISI PADA MEDIA PERBANYAKAN UNTUK MENINGKATKAN VIRULENSI BEAUVERIA BASSIANA TERHADAP HAMA WALANG SANGIT

2012 ◽  
Vol 12 (1) ◽  
pp. 64-70
Author(s):  
Ni Siluh Putu Nuryanti ◽  
Lestari Wibowo ◽  
Abdul Azis
Keyword(s):  
Beauveria Bassiana ◽  
Experimental Unit ◽  
Randomized Design ◽  
Rice Bug ◽  
Completely Randomized Design ◽  
Chitosan Powder

 A research was conducted to increase the virulence of Beauvaria bassiana to the rice bug, L.  acuta, by adding four different substances (materials) to the rice media to grow the fungus. The research was conducted from April to August 2011 at Polinela Laboratory, Bandar Lampung.  To do this a set of experimental unit consisting of five treatments and five replications were arranged in completely randomized design. The  four treatments were media made up of rice (rice based media) each given or added with four different materials such as rice skin powder, corn sugar, grasshopper powder and chitosan powder. The result showed that B. bassiana grown on rice media added with grasshopper powder or rice skin powder were able of causing mortality to the L. acuta as high as 78% and 71%, respectively, meaning that both mortalities were much higher than that of other replication. The virulence values of Beauveria bassiana  grown on those both powder were also higher than that of three others.

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