scholarly journals The Effect of Different pH in Extraction Process Against Physicochemical Properties of Refined Iota Carrageenan from Eucheuma spinosum Seaweed.

2020 ◽  
Vol 8 (1) ◽  
pp. 14
Author(s):  
Miftakhul Ulumiah ◽  
Mochammad Amin Alamsjah ◽  
Kustiawan Tri Pursetyo
Keyword(s):  
Acetic Acid ◽  
Ph Value ◽  
Chemical Properties ◽  
Extraction Process ◽  
Fish Bone ◽  
Weak Acid ◽  
Gel Strength ◽  
Partial Hydrolysis ◽  
Single Chain ◽  
Randomized Design

Gelatin is a simple protein obtained from the partial hydrolysis of collagen from the skin, bones, joints and connective tissue of animals. One potential material for the manufacture of gelatin is fish bone milkfish (Chanos chanos). Gelatin derived from fish bones with acid process is able to change the triple helix collagen fibers into a single chain, whereas the solvent base only produce double chain. Demineralization of bone or fish skin should use a weak acid is acetic acid, because when using strong acids will produce a strong scent and color gelatin becomes dark. This study aims to determine the effect of the use of acetic acid to the production of fish bone gelatin and the concentration of acetic acid is necessary to produce gelatin with physical and chemical properties of the best. This study uses a Completely Randomized Design (CRD) with four treatments and five replications. The treatments used in this study is the concentration of acetic acid 2%, 4%, 6% and 8%. Based on the research, it was found that the acetic acid solvent effect on the value of the yield and gel strength, but has no effect on the pH value and viscosity. Acetic acid concentration 8% give 4.102% yield value; pH value (acidity) 4.98; 134.313 bloom gel strength and viscosity of 3.6 cP. FTIR analysis results indicate that the fish bone gelatin produced from this research have the same functional group with commercial gelatin.

scholarly journals Ekstraksi Gelatin dari Limbah Kulit Kerbau (Bubalus bubalis) pada Berbagai Suhu Menggunakan Metode Asam

2017 ◽  
Author(s):  
masrukan
Keyword(s):  
Optimum Condition ◽  
Chemical Properties ◽  
Bubalus Bubalis ◽  
Gel Strength ◽  
Partial Hydrolysis ◽  
Physical And Chemical Properties ◽  
Randomized Design ◽  
Physical And Chemical ◽  
Hcl Solution ◽  
And Chemical Properties

Type A gelatin was extracted from partial hydrolysis of collagen from buffalo skin waste. The experiment was conducted to determine the effect of hydrochloride acid (HCl) solution and temperature extraction on physical and chemical properties of buffalo skin gelatin (BSG) and to determine optimum conditions for extraction of BSG which is based on the highest viscosity and gel strength. The experiment used completely randomized design (CRD) with two factors. The first factor was a concentration of HCl solution consisting of 3 levels (1, 2, and 3% v/v) and the second factor was temperature extraction (60, 70, and 80 oC). The results showed that concentration of HCl and temperature extraction significantly affect the physical and chemical properties of buffalo skin gelatin.The optimum condition is obtained using HCl 1% and temperature extraction of 70OC. Using optimum condition, the physical and chemical properties of BSG are: yield of 56.7%, Bloom gel strength of 140.72 gram; viscosity of 4.75 cP; melting point of 27.4°C; yellowish brown color; moisture content of 7.42%; protein content of 89.90%; fat content of 0.04%; ash content of 0.06%; and amino acid composition of glycine (22.15%),proline (11.07%) andalanine (11.20%).

scholarly journals PENGARUH PERBEDAAN KONSENTRASI LARUTAN ASAM ASETAT (CH3COOH) TERHADAP KARAKTERISTIK GELATIN KULIT KAKI AYAM

ZOOTEC ◽  
2015 ◽  
Vol 35 (2) ◽  
pp. 23
Author(s):  
Indri M. Pantow ◽  
Meity Sompie ◽  
Arie Dp. Mirah ◽  
Linda Ch. M. Karisoh
Keyword(s):  
Acetic Acid ◽  
Acid Solution ◽  
Ph Value ◽  
Acetic Acid Solution ◽  
Solution Concentration ◽  
Gel Strength ◽  
National Standard ◽  
Randomized Design ◽  
Chicken Legs ◽  
The Difference

THE EFFECT OF ACETIC ACID CONCENTRATION ON CHARACTERISTICS OF CHICKEN LEG SKIN GELATIN. This research was aimed to determine the effect of differenceacetic acid (CH3COOH) concentration on chicken legs skin gelatin. The experiment were determined by analysis of Completely Randomized Design with one factors and five replicates of treatments.The factor was concentration of acetic acid solution consisting 4 level (1, 3, 5 and 7%).The results showed that the difference  concentration acetic acid solution had high significant effect (P<0.01) on the the  yields, viscosity, gel strength and pH value ofchicken legs skin gelatin.Yield of chicken legskin gelatin was13.57 to 15.49%, viscocity was ranged between  4.30 to 5.76,  gel strength was 61.15 to 68.29 g Bloom and pH value was 5.24 to 5.58. The conclusion of this research was the chicken leg skin gelatin using acetic acid solution concentration 1 % to 7 % had the similar characteristics with the commercial gelatin and includedyields, viscosity, gel strength and pH value of Indonesian National Standard gelatin.   Key words : Acetic acid,  Gelatin and  Chicken legs skin

scholarly journals Peran Limbah Bahan Organik Pada Lubang Resapan Biopori Terhadap Sifat Kimia Tanah Di Lahan Pasca Tambang

2020 ◽  
Vol 16 (3) ◽  
pp. 439
Author(s):  
Raziv Rahman ◽  
Emmy Sri Mahreda ◽  
Basir Basir ◽  
Bdaruddin Badaruddin
Keyword(s):  
Soil Fertility ◽  
Land Reclamation ◽  
Ph Value ◽  
Liquid Waste ◽  
Chemical Properties ◽  
Biological Properties ◽  
Organic Matters ◽  
Run Off ◽  
Randomized Design ◽  
Completely Randomized Design

Biopore infiltration hole are hole made for run-off flow which if allowed to cause erosion and can remove the topsoil, causing soil fertility to be reduced. The use of biopori infiltration holes can be maximized by adding organic matters into the hope that in addition to absorbing water it can increase soil fertility. The liquid waste of tofu and meranti litter is an organic matters are used in this study, by adding organic matters to the biopori hole. This study aims to look at the effect of providing organic matters in the biopore hole derived from tofu and litter liquid waste on the value of pH, C-Organic, and post-mining CEC. This study was an experimental study in the field using a completely randomized design of one factor, namely organic matters with six levels, namely without organic matters and using 25 g, 50 g, 75 g, and 100 gr organic matters given to the biopori hole in incubation for one month later Soil samples taken were analyzed for pH, C-organic, and CEC. The results showed that the treatment only affected the soil's pH value. Organic material provided in the biopori hole is thought to have not completely decomposed, causing the soil around the biopori infiltration hole to remain acidic. The acidity of the soil is due to the gases produced during the decomposition process. Changes in soil pH ranged from 4.36 to 4.65 by administering 75g of organic matters from tofu liquid waste and meranti litter. This research is useful in post-mining land reclamation in increasing soil fertility, with the improvement of soil chemical properties such as pH will slowly improve the physical and biological properties of the soil so that it can be used as cultivation land.

scholarly journals Effectiveness of Alkali and Acid to Produce Collagen from Fish Skin of Striped Catifish

2017 ◽  
Vol 20 (2) ◽  
pp. 255
Author(s):  
Hilda Lu’lu’in Nanda Alfira Devi ◽  
Pipih Suptijah ◽  
Mala Nurilmala
Keyword(s):  
Acetic Acid ◽  
Type I Collagen ◽  
Electrophoretic Analysis ◽  
Extraction Process ◽  
Skin Extract ◽  
Type I ◽  
Fish Skin ◽  
Soaking Time ◽  
Randomized Design ◽  
Striped Catfish

Fish skin is one of the alternative sources contained high protein  to isolate collagen. Fish skin generally extracted by the method of acid, alkali and enzymes. The study aim to determine the effectiveness of NaOH<br />and acetic acid on catfish (Pangasius sp.) skin extraction  process.  The concentrations of alkaline pretreatment were 0,05; 0,1; 0,15 and 0,2 M with the soaking time of 2, 4, 6, 8 and 10 h by NaOH replacement in every 2 h. The concentrations of acetic acid for hydrolisis process were 0.05; 0.1; 0.15 and 0.2 M with the soaking time of 1, 2, and 3 h. The experimental design used for pretreatment process is split splot, while for the hydrolysis process is factorial completely randomized design. The results showed that pretreatment with a concentration of 0.05 M NaOH for 4 h has a significant effect for eliminating non-collagen protein (p&lt;0.05). The acetic acid concentration of 0.15 M for 1 h also has a significant effect on fish skin swelling. The yield of striped catfish collagen was 17.272%, the protein content was 86%, and the viscosity was 12 cP. Fish skin extract was identified as type I collagen by functional groups and electrophoretic analysis. Collagen from striped catfish skin has α1 and α2 and protein structure with the molecular weight of α chain were 94 and 98 kDa, meanwhile the molecular wheight of β chain was 204 kD.

scholarly journals VARIASI WAKTU EKSTRAKSI DAN JENIS ASAM PADA PROSES PRODUKSI GELATIN TULANG IKAN NILA (Oreochromis niloticus)

2020 ◽  
Vol 7 (2) ◽  
pp. 65-69
Author(s):  
Neneng Suliasih ◽  
Asep Dedy Sutrisno ◽  
Nabella Respatyana
Keyword(s):  
Acetic Acid ◽  
Protein Content ◽  
Research Result ◽  
Extraction Process ◽  
Gel Strength ◽  
Ash Content ◽  
Extraction Time ◽  
Main Research ◽  
Physical Response ◽  
Main Plot

The purpose of this research is to find out determine the effect of the most effective extraction time and to determine the effect of the most effective type of acid in the production process of gelatin bone of tilapia fish. This research was using by Split Plot Design (SPD) with 3 times replication, so make 27 experiments were obtained. For main plot is extraction time (4 hours, 5 hours and 6 hours) and subplot is type of acid (4% chloride acid, 3% acetic acid and 6% citrate acid). Response in this research is physical response such as gel gelatin strength, viscosity and rendement total, chemical response such as pH, protein content and ash content and test of bone gelatin of tilapia fish with commercial gelatin. The main research result showed that the extraction time had significant effect on gelatin gel strength, gelatin viscosity, gelatin rendement, acidity degree (pH) of gelatin, gelatin protein content and gelatin ash content. Types of acid had significant effect on gelatin gel strength, gelatin renedement, acidity degree (pH) of gelatin, gelatin protein content and gelatin ash content, and interaction of time of extraction and type of acid had significant effect on gelatin gel strength, gelatin viscosity, gelatin rendement, acidity degree (pH) of gelatin, gelatin protein content and gelatin ash content to the extraction process of bone gelatin of tilapia fish. Based on the comparative test, the characteristics of gelatin of tilapia bone that close to commercial gelatin are a3b2 treatment (6 hours of extraction time, 3% acetic acid) with the result of gelatin gel strength 132,93 bloom; viscosity 5,83 cP; the amount of rendement 16,94; pH 6,10; protein content 83,62%; and ash content of 0.64%.

scholarly journals Profiling of Collagens from Swim Bladder of Catfish (Pangasius sp.) by Acid Extraction

2019 ◽  
Vol 22 (2) ◽  
pp. 327-339
Author(s):  
Gressty Sari Br Sitepu ◽  
Joko Santoso ◽  
Wini Trilaksani
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Triple Helix ◽  
Extraction Process ◽  
Raw Material ◽  
Acid Extraction ◽  
Swim Bladder ◽  
Acid Pretreatment ◽  
Randomized Design ◽  
Completely Randomized Design

Swim bladders of catfish contain high protein, therefore it is can be used as a raw material for collagen. The study aims to determine the chemical characteristics of swim bladders, the pretreatment of non-collagen, extraction of collagen dissolves acid and to evaluate the characteristics of collagen. The method of this study is KOH pretreatment with a concentration of 0,05; 0,1; and 0,15 for 12 hours. The extraction process is done by soaking the sample in a solution of acetic acid with a concentration of 0,25; 0,5 and 0,75 M (ratio 1:10; b/v) and extraction time for 24; 48; and 72 hours at 4oC. The experimental design used for alkaline and acetic acid pretreatment were factorial completely randomized design. The result showed that the protein content of swim bladder was 85,26% (db), the profile of amino acids were dominated by three amino acids namely glycine (56,85 mg/g), prolyne (31,03 mg/g), and alanyne (23,85 mg/g). Using 0,05 M KOH for 8 h was selected as the best pretreatment method for collagen extraction. Extraction method using 0,50 M acetic for 48 h resulted he best collagen which revealed the existence of a triple helix structure and had Tg 84oC.

scholarly journals Effects of organic acid on the physicochemical properties of gelatine extracted from Fringescale sardinella (Sardinella fimbriata) bones

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 294-302
Author(s):  
N.M. Arshad ◽  
M. Abd. Ghaffar ◽  
N.F. Mohtar
Keyword(s):  
Melting Point ◽  
Physicochemical Properties ◽  
Organic Acid ◽  
Chemical Properties ◽  
Extraction Process ◽  
Gel Strength ◽  
Inorganic Acid ◽  
Alternative Source ◽  
Physico Chemical ◽  
By Products

Gelatine from marine sources especially from the fish becomes a popular alternative to replace the commercial gelatines from bovine and porcine. Fringescale sardinella (Sardinella fimbriata) is commonly used in the fish processing industry and has a high potential to produce gelatine from its by-products. The extraction of gelatine is one of the most important steps that influence its functional properties. Inorganic acid was commonly used in the extraction, however, nowadays it faces some issues due to health safety and its effect on the environment. The aim of this study was to assess the effects of organic acid (fruit vinegar) as a replacement for inorganic acid (hydrochloric acid) during extraction on the physicochemical properties of S. fimbriata gelatine. The extracted gelatines were characterized in terms of their physico-chemical properties such as the yield percentage, gel strength, melting point, molecular weight, structural composition, and amino acid analyses. Results showed that gelatine extracted with organic acid demonstrated low yield percentage (2.9±0.01%) and poor gel strength (75.9±1.06 g) and melting point (22.0±1.00°C) values compared to the standard. This gelatine also demonstrated a low amount of proline and hydroxyproline contents, which were 43.77±8.21 g/mg and 34.99±6.50 g/mg, respectively. Overall findings have suggested that different types of acid used in the extraction resulted in different physico-chemical properties of the gelatine. Such findings suggested that S. fimbriata bone hold the potential to be an alternative source for vast applications in industries. Further studies may focus on different concentrations of acid used in the extraction process. Such findings will help to improve S. fimbriata bone gelatine properties for extended uses in various industries.

scholarly journals PENGARUH PERBEDAAN KONSENTRASI LARUTAN ASAM ASETAT TERHADAP NILAI KEKUATAN GEL, VISKOSITAS, KADAR PROTEIN, DAN RENDEMEN GELATIN KULIT SAPI

ZOOTEC ◽  
2020 ◽  
Vol 40 (2) ◽  
pp. 593
Author(s):  
Indriani Wewengkang ◽  
M. Sompie ◽  
S.E. Siswosubroto ◽  
J.H.W. Pontoh
Keyword(s):  
Acetic Acid ◽  
Acid Solution ◽  
Protein Content ◽  
Acetic Acid Solution ◽  
Gel Strength ◽  
Randomized Design ◽  
Completely Randomized Design

THE EFFECT OF DIFFERENT CONCENTRATION OF ACETIC ACID SOLUTION ON THE GEL STRENGTH, VISCOSITY, PROTEIN CONTENT AND RENDEMEN VALUEThis research conducted to determine the effect of different concentration of acetic acid solution on the gel strength, viscocity, protein content and rendemen of cow skin gelatin. The material used cowskin and acetic acid solution. This study used Completely Randomized Design (CRD). The treatments were concentration of acetic acid solution (1%, 3%, 5% and 7%) and  four replicates of treatment. The result showed that the different concetration of acetic acid solution  had no significant  effect (P>0.05) to the gel strength and rendemen value of gelatin, but it had significant effect (P<0.05) on viscosity and protein content of cow skin gelatin. It was concluded that  acetic acid solution 3% had  the best gel strength, viscosity, protein content  and rendemen value of cow skin gelatin.Keywords: Acetic acid, Cow skin, Gelatin

scholarly journals Review Article: Fish Bone Collagen

2021 ◽  
pp. 33-39
Author(s):  
Andre Wijaya ◽  
. Junianto
Keyword(s):  
Type I Collagen ◽  
Ph Value ◽  
Amino Acid Content ◽  
Extraction Process ◽  
Fish Bone ◽  
Raw Material ◽  
Bone Collagen ◽  
Type I ◽  
Alternative Source ◽  
Salting Out

Fishbone collagen is an alternative source of bovine and pig collagen. The purpose of this article is to review the types, benefits, extraction methods and characterization of collagen from fish bones, especially freshwater fish. The result of the review shows that the collagen from tilapia bones is shaped like a sheet with a slight porous surface and is of type I. Collagen extraction begins with bone disease from the remaining fat, then degreasing and the next step is extraction. After the extraction process is complete, filtration is carried out to store the filtrate or residue, then the collagen is purified by salting-out with NaCl. Collagen yield and collagen pH value from collagen extraction were different for each type of fish bone. The difference in yield value and protein and amino acid content in the resulting collagen can be caused by differences in the extraction method, the concentration of the solution used, whether acid or alkaline, and the neutralization process and the type of raw material. Fishbone collagen has good benefits in the cosmetic and pharmaceutical fields.

scholarly journals Characteristics of Bone Gelatin Tilapia (Oreochromis niloticus) Processed by Using Hydrolysis With Phosphoric Acid and Papain Enzyme

2016 ◽  
Vol 19 (1) ◽  
pp. 69
Author(s):  
Gugun Hidayat ◽  
Eko Nurcahya Dewi ◽  
Laras Rianingsih
Keyword(s):  
Phosphoric Acid ◽  
Ph Value ◽  
Physicochemical Characteristic ◽  
Fish Bone ◽  
Gel Strength ◽  
Second Phase ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Difference Test ◽  
Honestly Significant Difference Test

<p>Phosphoric acid and papain enzyme able to hydrolyzing collagen from Tilapia into gelatin . The<br />purpose of this research was to determine the best concentration of phosphoric acid and papain enzyme<br />and to determine the physicochemical characteristic gelatin to from Tilapia fish bone which processed<br />with phosphoric acid and papain enzyme. The first research phase was making bone gelatin tilapia using<br />phosphoric acid at concentration of 4%, 5% and 6%, and the papain enzyme 0.5%, 1% and 1.5%. The<br />second phase was characterize the physicochemical gelatin from the best concentration of phosphoric acid<br />concentration (6%) and papain enzyme (1.5%), all treatment done with three repetitions. Analysis of the<br />data using ANOVA with completely randomized (CRD) design If there was difference between treatment<br />then continued with Honestly Significant Difference Test (HSDT). The results of the first research phase<br />found the best concentration were 6% of phosphoric acid and 1.5% papain enzyme, its shows by the value<br />gel strength 325,95 and 373,32 g.bloom. The second research phase shows that the the best results obtained<br />in this study was gelatin from 1.5% papain enzyme as hydrolysis agent, the physicochemical characteristic<br />were: 376.21 g.bloom gel strength; viscosity of 7.57 cP; yield 6.30%; protein content of 86.46%; water content<br />of 7.12%; and the pH value of 5.11.<br />Keywords : gelatin, hydrolysis, papain enzyme, phosphoric acid, tilapia bones</p>

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