scholarly journals The Combination Effect of Simvastatin and Virgin Coconut Oil on Total Cholesterol Levels in Dislipidemic Male Albino Rats (Rattus norvegicus)

2021 ◽  
Vol 12 (2) ◽  
pp. 72
Author(s):  
Lutfi Dewanda Nugroho ◽  
Soebagijo Adi Soelistijo ◽  
Jusak Nugraha
Keyword(s):  
Treatment Group ◽  
Total Cholesterol ◽  
Total Cholesterol Level ◽  
Coconut Oil ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Cholesterol Levels ◽  
Group I

Introduction: Simvastatin is a primary pharmacological therapy for dyslipidemia (hypercholesterolemia). Virgin coconut oil (VCO) is a companion intake of statin drugs considered capable of lowering blood cholesterol levels. This study aimed to determine the combination effect of statin drug groups with VCO on total cholesterol.Methods: The stages of this study started with the adaptation process of animals, giving a high-fat diet (HFD), and treatment with simvastatin and VCO. The adaptation process was conducted where the experimental animals were fed pellets and drank clean water in a container. A HFD was given by mixing pork brains with distilled water with a ratio of 1:3 as much as 2 ml per day for 21 days. In simvastatin and VCO treatment for 7 days, the experimental animals were divided into 4 groups; group I was given aquades, group II was given VCO, group III was given simvastatin, and group IV was given simvastatin and VCO. The total cholesterol levels in the blood of albino rats were examined by employing a BS-300 Chemistry Analyzer through a spectrophotometric method. The total cholesterol levels data obtained were then examined for normality (Shapiro-Wilk test) and analyzed parametrically by utilizing One-way ANOVA.Results: The average total cholesterol level in the negative control group (being given a standard diet) was 45.57 mg/dL. The averages of blood total cholesterol levels were 47.28 mg/dL, 45.00 mg/dL, 46.85 mg/dL, 41.42 mg/dL, and 44.28 mg/dL in the positive control group (given an HFD), in the treatment group I, in the treatment group II, in the treatment group III, and in the treatment group IV, respectively. There were no significant distinctions in the statistical test on decreasing blood total cholesterol among the negative control group, positive controls I, II, III, and IV.Conclusion: Combination of simvastatin and VCO tends to reduce the total cholesterol level in albino rats. This is better than just giving VCO simvastatin alone.

Aktivitas Antihiperkolesterolemia Ekstrak Etanol Daun Stevia Rebaudiana Bertoni Pada Tikus Putih Jantan

2020 ◽  
Vol 2 (1) ◽  
pp. 11-20
Author(s):  
Meta Kartika Untari ◽  
Ganet Elo Pramukantoro
Keyword(s):  
Total Cholesterol ◽  
Total Cholesterol Level ◽  
Density Lipoprotein ◽  
Stevia Rebaudiana ◽  
Negative Control ◽  
Stevia Rebaudiana Bertoni ◽  
Cholesterol Levels ◽  
Water Solvent ◽  
White Rats ◽  
Group I

Hypercholesterolemia is a state of increased levels of LDL (Low Density Lipoprotein) and total cholesterol in the plasma. Stevia leaves have benefits to overcome hypercholesterolemia. The aimed of this study was to obtain ethanol extracts of Stevia rebaudiana Bertoni leaves which have activity to reduce total cholesterol levels in patients with hypercholesterolemia with effective doses. The method that will be carried out to achieve this goal was to make extracts by maceration of Stevia rebaudiana Bertoni leaf powder using a water solvent for 5 days. Testing antihypercholesterolemia activity by giving treatment to 20 male white rats. Rats were divided into 5 treatment groups. Group I was negative control, II was simvastatin control, III extract was 30 mg / 200 g BW, IV extract was 60 mg / 200 g BW, V extract was 120 mg / 200 g BW. The mice were induced by propylthiouracil 12.5 mg / day and high-fat feed for 21 days, after which the rats were given the test for 14 days. Cholesterol levels were measured on days 0, 21st and 28th. The method of determining cholesterol levels uses the Easy Touch tool. On the 35th day, a total cholesterol level was examined and data analysis was performed. The results showed that the ethanol extract of stevia leaves had antihypercholesterolemia activity, extract dose of 30 mg / 200 g BW had antihypercholesterolemia activity which was equivalent to simvastatin.

scholarly journals Efektivitas Ekstrak Artemisia vulgaris L. sebagai Hepatoprotektor pada sel-sel Hati Tikus yang Diinduksi Niasin

2018 ◽  
Vol 6 (2) ◽  
pp. 251
Author(s):  
Laily Rahmawati ◽  
Erma Sulistyaningsih ◽  
Rosita Dewi
Keyword(s):  
Treatment Group ◽  
Liver Damage ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Artemisia Vulgaris ◽  
Group I ◽  
Group Ii

  The niacin in energy drinks has metabolic product that cause oxidative stress and liver damage, while the liver damage can be prevented by hepatoprotective agents. Scoparone in Artemisia vulgaris L. can act as a hepatoprotector by its antioxidant effect. This study aimed to investigate the effectivity of Artemisia vulgaris L. extract as a hepatoprotector in wistar hepatocytes induced by niacin. This study used 25 male rats which were divided into 5 groups: normal, the negative control, the positive control, the treatment group I, and II. Treatment was conducted for 28 days. The samples were terminated and the hepatocyte were prepared for histological examination. Histological appearance was catagorized as mild, moderate, and severe damage with or without inflamatory cells activity. The data analysis by Kruskal Wallis showed significant difference (p<0,001). Further analysis by Mann Whitney revealed significantly difference (p<0,05) between normal group and all groups, negative control group and positive control group, and positive control group and treatment group I, but not significantly difference between negative control group and treatment group I, negative control group and treatment group II, positive control group and treatment group II, and between treatment groups. The study concluded that the effectivity of Artemisia vulgaris L. extract has not been proven as a hepatoprotector but further study is needed to draw a definite conclusion.   Keywords: energy drink, niacin, Artemisia vulgaris L., hepatoprotector  

scholarly journals UJI EFEK EKSTRAK ETANOL DAUN JAMBU BIJI (Psidium guajava L) TERHADAP KADAR KOLESTEROL TOTAL TIKUS WISTAR (Rattus norvegicus)

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Irianto Girik Allo ◽  
Pemsi Mona Wowor ◽  
Henoch Awaloei
Keyword(s):  
Total Cholesterol ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Psidium Guajava ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Cholesterol Levels ◽  
Guava Leaves ◽  
Guava Leaf

Abstract: This research aims to determine the effect of ethanol extract of guava leaves on levels of total cholesterol of rat that is induced propylthiouracil. This was an experimental research. The sample used was 15 rats were divided into 5 groups (1 group is consist of 3 rats), namely: 1) positive control, 2) extract dose of 200 mg/kgBB, 3) extract dose of 400 mg/kgBB, 4) extract dose of 800 mg/kgBB and 5) negative control. Group 1 to 4 are given propylthiouracil given for 14 days, then given treatment appropiate with the group. The result show that: 1) the average of total cholesterol levels increased after administration propylthiouracil from 156.34 mg/dL to 247.84 mg/dL, 2) at a dose of 200 mg/kgBB, 400 mg/kgBB and 800 mg/kgBB showed a decline in total cholesterol levels by 32.73%, 58.73% and 47.26%, 3) the positive control decreased total cholesterol levels by 39.46% and 4) total cholesterol levels on negative is unstable (there are up and some are down). The result of this research concluse that: guava leaf extract at dose of 200 mg/kgBB, 400 mg/kgBB and 800 mg kgBB have an effect in lowering total cholesterol of rat had been induced prophylthiouracil. Keywords: guava leaves, total cholesterol.     Abstrak: Penelitian ini bertujuan untuk mengetahui efek dari ekstrak etanol daun jambu biji terhadap kadar kolesterol total tikus wistar yang diinduksi propiltiourasil. Penelitian ini merupakan penelitian eksperimental. Sampel yang digunakan berjumlah 15 tikus wistar yang dibagi menjadi 5 kelompok (1 kelompok terdiri dari 3 tikus wistar), yaitu: 1) kontrol positif, 2) ekstrak dosis 200 mg/kgBB, 3) ekstrak dosis 400 mg/kgBB, 4) ekstrak dosis 800 mg/kgBB dan 5) kontrol negatif. Kelompok 1 sampai 4 diberikan propiltiourasil selama 14 hari, kemudian diberikan perlakuan sesuai kelompoknya. Hasil penelitian yang diperoleh: 1) rata-rata kadar kolesterol total meningkat setelah pemberian propiltiourasil yaitu dari 156,34 mg/dL menjadi 247,84 mg/dL, 2) pada dosis 200 mg/kgBB, 400 mg/kgBB dan 800 mg/kgBB menunjukkan terjadi penurunan kadar kolesterol total sebesar 32,73%, 58,73% dan 47,26%, 3) pada kontrol positif terjadi penurunan kadar kolesterol total sebesar 39,46% dan 4) kadar kolesterol total pada kelompok negatif tidak stabil (ada yang naik dan ada yang turun). Hasil penelitian ini menyimpulkan bahwa ekstrak daun jambu biji pada dosis 200 mg/kgBB, 400 mg/kgBB dan 800 mg/kgBB mempunyai efek dalam menurunkan kadar kolesterol total tikus wistar yang telah diinduksi propiltiourasil. Kata kunci: daun jambu biji, kadar kolesterol total.

scholarly journals 4. The Value of Erythrocytes, Hemoglobin, and Hematocrit of Mice (Mus musculus) Exposed to Cigarette Smoke and Given Red Watermelon Extract (Citrullus vulgaris)

2018 ◽  
Vol 12 (1) ◽  
pp. 24-31
Author(s):  
Yola Heryanita ◽  
Rusli Rusli ◽  
Rosmaidar Rosmaidar ◽  
Zuraidawati Zuraidawati ◽  
Rinidar Rinidar ◽  
...  
Keyword(s):  
Treatment Group ◽  
Cigarette Smoke ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Citrullus Vulgaris ◽  
Randomized Design ◽  
Group I ◽  
The Given

The aim of this research was to investigate the effect of red watermelon (Citrullus vulgaris) extract to the value of erythrocytes, hemoglobin, and hematocrit of mice (Mus muscullus) exposed to cigarette smoke. This study followed direct complete randomized design by using 12 males’ mice which divided into 4 groups. The treatment group is divided by the negative control group which given 0,5 ml of aquadest, the positive control group which exposed to cigarette smoke and 0,5 ml of aquadest. The treatment group I was exposed to cigarette smoke and given red watermelon extract dose 22 mg/Bw mice. The treatment group II was exposed to cigarette smoke and given red with watermelon extract dose 44 mg/Bw mice. The exposure to cigarette smoke and the given of red watermelon extract were conducted for 30 days. The blood taking was performed on day 31 in the Plexus retroorbital.  Furthermore, the number of erythrocytes, hemoglobin and hematocrit were calculated. The data were analyzed using ANOVA completely randomized design with SPSS for Windows 16.0. The results of this study showed that the extract of red watermelon for 30 days showed a highly significant effect (P 0.01) to the number of erythrocytes, hemoglobin, and hematocrit of mice. It is concluded that free radicals contained in the cigarette can cause a hemoglobin desaturation, increased blood viscosity, oxidative stress and red watermelon extract can minimize the damage.

scholarly journals Pengaruh susu kedelai dan jahe terhadap kadar kolesterol total pada wanita hiperkolesterolemia

2016 ◽  
Vol 4 (2) ◽  
pp. 89-95
Author(s):  
Deny Yudi Fitranti ◽  
Diassafons Marthandaru
Keyword(s):  
Treatment Group ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
Total Cholesterol Level ◽  
Intervention Group ◽  
Control Group ◽  
Soy Milk ◽  
Cholesterol Levels ◽  
Before And After ◽  
And Control

Background: Hypercholesterolemia is one of the risk factor of coronary heart disease. Soy contains protein, fiber and isoflavones which can reduce total cholesterol levels. Soy milk added by ginger might increase antioxidant activity. Ginger consist of oleoresin that can  reduce total cholesterol level.Objective: To investigate the effect of soy milk and ginger to the total cholesterol levels in hypercholesterolemic women Methods: The study was true experiment with the control group pre-post test design. Subjects were 16 women aged 30-55 years with serum total cholesterol level 200-250 mg / dl. Subject were divided into two group. The intervention group receive 430 ml soy milk with 3 gram ginger and control group receive placebo. Total cholesterol level was measured by CHOD-PAP method. Food intake during the intervention was measured using food records and 24-hours food recall.. Data was analyzed by using  Independent t test, Wilcoxon and Multiple Linear Regression.Results: Mean of total cholesterol level in treatment group decreased 5,50 mg/dl and the placebo group had increased total cholesterol l9.44 mg/dl. There were no differences in total cholesterol levels before and after intervention in the treatment group (p = 0.204) and control group (p = 0.093). There were differences in variations in total cholesterol levels between the two groups (p = 0.041). Conclusion: Consumed soy milk with ginger as much as 430ml/day during 14 days there was no difference in total cholesterol levels before and after intervention with decreased total cholesterol by 5,50 mg/dl.

scholarly journals ANREDERA CORDIFOLIA LEAVES FRACTION AS AN ANTI-HYPERLIPIDEMIA

2016 ◽  
Vol 9 (6) ◽  
pp. 82 ◽  
Author(s):  
Dwintha Lestari
Keyword(s):  
Ethyl Acetate ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
Total Cholesterol Level ◽  
Ethyl Acetate Fraction ◽  
Negative Control Group ◽  
Negative Control ◽  
Blood Cholesterol ◽  
Control Group ◽  
Water Fraction

Objective: The objective of this research was to evaluate reduction of blood cholesterol level by fraction extracts of binahong (Anredera cordifolia) on male Wistar rats. Methods: The rats were given high fat food every day for 3 months and pure cholesterol on week 0, 3, 7 and 11.  After that each binahong fraction (n-hexane fraction, ethyl acetate fraction and water fraction) with doses of 50 mg/kg bw, 100 mg/kg bw, 200 mg/kg bw were given orally for 3 weeks and observed for lipid profile on day 21.   Results: The result showed that n-hexane, ethyl acetate and water fractions of binahong leaves decreased total cholesterol, triglyceride and LDL level, and did not influence the HDL level. Meanwhile statin decreased total cholesterol, triglyceride and LDL level, and increased HDL level. Conclusion: Ethyl acetate fraction of binahong leaves significantly reduced total cholesterol level, triglycerides, LDL level compared to negative control group (p< 0.05) and had no influence in HDL level. Keywords: Atherosclerosis, Anredera cordifolia, cholesterol

scholarly journals Gambaran histologik hati tikus Wistar yang diberi jus tomat setelah diinduksi monosodium gluatamat

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Christantio Legoh ◽  
Martha M. Kaseke ◽  
Taufiq F. Pasiak
Keyword(s):  
Treatment Group ◽  
Wistar Rats ◽  
Inflammatory Cells ◽  
Fatty Degeneration ◽  
Negative Control Group ◽  
Negative Control ◽  
Tomato Juice ◽  
Control Group ◽  
Group I ◽  
Group Ii

Abstract: Monosodium glutamate (MSG) is one of the ingredients used as flavour additive. However, excessive consumption of MSG could damage the liver. This study was aimed to obtain the impact of MSG exposure on histopathological findings of the liver of Wistar rats administered with tomato juice. This was an experimental study with the post-test only control group design. Subjects were Wistar rats divided into three groups, as follows: the negative control group, treatment group I, and treatment group II. The negative control group was given AD2 pellets; the treatment group I was given AD2 pellets and MSG, while the treated group II was given MSG and tomato juice. This study was conducted for 14 days. The histopathological examination of the treatment group I showed fatty degeneration of hepatocytes and infiltration of inflammatory cells meanwhile of the treatment group II, there was less fatty degeneration of hepatocytes compared to the treatment group I and no inflammatory cells. Moreover, there was no fatty degeneration and inflammatory cells in the negative control group. Conclusion: Wistar rats treated with MSG showed fatty degeneration of hepatocytes and infiltration of inflammatory cells which could be reduced by administration of tomato juice along with MSG.Keywords: tomato juice, MSG Abstrak: Monosodium glutamat (MSG) merupakan salah satu bahan makanan yang sering digunakan sebagai penyedap rasa namun konsumsi MSG berlebihan dapat merusak hati. Penelitian ini bertujuan untuk mengetahui pengaruh pajanan MSG terhadap gambaran histologik hati tikus Wistar dengan dan tanpa disertai pemberian jus tomat. Jenis penelitian ialah eksperimental dengan post-test only control group design. Tikus dibagi menjadi 3 kelompok, yaitu kelompok kontrol negatif, kelompok perlakuan I, dan kelompok perlakuan II. Kelompok kontrol negatif hanya diberikan pelet AD2; kelompok perlakuan 1 diberikan pelet AD2 dan MSG; kelompok perlakuan 2 diberikan MSG dan jus tomat. Penelitian ini dilakukan selama 14 hari. Hasil pengamatan gambaran histologik hati tikus Wistar pada kelompok perlakuan I mendapatkan adanya degenerasi lemak pada hepatosit dan sel-sel radang PMN. Pada kelompok perlakuan 2 masih ditemukan adanya degenerasi lemak pad hepatosit tetapi dengan jumlah yang lebih kurang daripada kelompok perlakuan I, serta tidak ditemukan sel-sel radang PMN. Pada kelompok kontrol negatif tidak ditemukan adanya degenerasi lemak pada hepatosit dan sel radang. Simpulan: Pada tikus wistar dengan pemberian MSG terdapat degenerasi lemak pada hepatosit dan adanya sel-sel radang. Pemberian jus tomat bersamaan dengan pemberian MSG berefek menurunkan terjadinya degenerasi lemak pada hepatosit dan tidak disertai sel-sel radang.Kata kunci: jus tomat, MSG

scholarly journals Pengaruh Suplementasi Madu Trigona terhadap Parameter Fungsi Hati dan Ginjal Tikus Albino (Rattus norvegicus) yang Diberikan Simvastatin

2018 ◽  
Vol 4 (1) ◽  
pp. 36-43
Author(s):  
Sukamto Salang Mamada ◽  
Usmar Usmar ◽  
Aliyah Aliyah ◽  
Aminullah Aminullah ◽  
Ayu Indah Rahayu ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Lipid Level ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Group V ◽  
Group I ◽  
Significant Difference ◽  
Coa Reductase

Simvastatin is a drug acting on 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase enzyme leading to decrease of lipid level in plasma. Simvastatin is associated with pleiotropic effects such as cardioprotective, hepatoprotective, and nephroprotective effect. This study aimed to observe effect of supplementation of trigona honey on parameters of liver function (SGPT and SGOT) and kidney function (urea) in albino rats (Rattus norvegicus) given 40 mg/kg simvastatin. Twenty-four male albino rats were divided into 6 groups (n=4). Each group was administered different treatments for 15 days orally. Group I was put as health control without any treatment, group II was given sodium carboxymethylcellulose (1% b/v) as negative control, group III was given simvastatin at the dose of 40 mg/kg, group IV was administered simvastatin (40 mg/kg) and trigona honey (6.5% v/v), while group V and VI were administered simvastatin (40 mg/kg) and ubiquinone (1.43 mg/kg); and simvastatin (40 mg/kg), trigona honey (6.5% v/v), and ubiquinone (1.43 mg/kg), respectively. Upon the treatments, level of SGOT, SGPT, and ureum was determined. The data were analyzed by using Analysis of Variance (ANOVA) and Least Significant Difference tests (p=0.05). According to the analysis, it was concluded that supplementation of trigona honey in rats administered simvastatin showed significantly lower level of all parameters than groups of simvastatin and controls.

scholarly journals Antihyperlipidemic Activity of Ethanol Extract Mindi’s Leaves (Melia azedarach Linn.) in Male Wistar Rats Induced Propiltiouracil

2019 ◽  
Vol 4 (1) ◽  
pp. 24
Author(s):  
Herlina Herlina ◽  
Budi Untari ◽  
Indah Solihah ◽  
Merie Santia
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Ethanol Extract ◽  
Negative Control ◽  
Melia Azedarach ◽  
Control Group ◽  
Albino Rats ◽  
Group Iii ◽  
Group I ◽  
Antihyperlipidemic Activity

Mindi’s leaves (Melia azedarach Linn.) is an Indonesian medicinal plant that used as traditional medicine. Mindi leaves contains some secondary metabolites which have potency to decreased total cholesterol and LDL level. The study purposed to know antihyperlipidemic effect of ethanol extract of mindi’s leaves (Melia azedarach Linn.) seen from total cholesterol, and LDL in male albino rats. Male wistar albino rats were divided into 5 groups, negative control group (sodium CMC 0.5%), positive control group (simvastatin 0.193 mg/200 gBW), group I (ethanol extract mindi’s leaves with dosage 300 mg/200 gBW), group II (ethanol extract mindi’s leaves with dosage 600 mg/200 gBW), and group III (ethanol extract mindi’s leaves with dosage 1200 mg/200 gBW). The rats were given high-fat supplement and propylthiouracil for 15 days to increase cholesterol, and the extract was given for the next 15 days. Average cholesterol level, LDL, and body weight after induction was 90.28 mg/dL, 31.09 mg/dL, and 222.32 g. The result showed ethanol extract mindi’s leaves could decreased total cholesterol level and LDL level with % decreased in total cholesterol (%PDTC) and % decreased in LDL (%PDLDL) of group I is 37.78% and 35.57%, group II is 45.99% and 40.39%, and for group III is 56.29% and 52.42%. The result showed that ethanol extract of mindi’s leaves has antihyperlipidemic activity and significantly different from negative control (p>0.05). Based on the percentage relation of decreased total cholesterol and LDL levels to dose, then the effective dose 50 (ED50) value of ethanol extract mindi’s leave is 869 mg/200 gBW for total cholesterol and for LDL reduction level is 1086.84 mg/200 gBW

scholarly journals Evaluation of Protective Properties of Elaeis oleifera Fruit Extract on Renal Parameters of Dichlorvos-Induced Nephrotoxocity in Albino Rats

2020 ◽  
pp. 1-13
Author(s):  
Edna Ogechi Nwachuku ◽  
Fedelis Beega ◽  
Adline Erinma Ben- Chioma ◽  
Ngozi Brisibe ◽  
Ibioku Elekima
Keyword(s):  
Treatment Group ◽  
Palm Oil ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Albino Rats ◽  
Study Group ◽  
Acute Study

Aim: Evaluate the protective effects of palm oil on renal parameters after dichlorvos toxicity in albino rats. Study Design and Methodology: The study consisted of 3 phases: The acute study which lasted for 24 hours, the sub-acute study which lasted for 14 days and the sub chronic study which lasted for 30 days. The design and treatment pattern is shown below. Phase 1: Acute Study. Group 1: No DDVP, No palm oil for 24 hours (Negative control), Group 2: 30 mg/kg of DDVP without palm oil (positive control), Group 3: 30 mg/kg of DDVP and 100 mg/kg palm oil for 24 hours (treatment group). Phase 2: Sub-Acute (14 days) Study. Group 4: No DDVP, No palm oil for 14 days (Negative control), Group 5: 10 mg/kg of DDVP without palm oil daily for 14 days (positive control), Group 6: 10 mg/kg of DDVP and 100 mg/kg of palm oil daily for 14 days (positive control). Phase 3: Sub-Chronic (30 days) Study. Group 7: No DDVP, No palm oil for 30 days (Negative control), Group 8: 10 mg/kg of DDVP without palm oil daily for 30 days (positive control), Group 9: 10 mg/kg of DDVP and 100 mg/kg palm oil daily for 30 days (treatment group). All administration was done orally. After the period of treatments, the rats were sacrificed after 18 hours of fast. Whole blood samples (5 mls) were collected into lithium heparin bottle and spun at 3500 rpm for 5 minutes to obtain plasma samples. Samples obtained were used for the determination of Na+, K+, HCO3, urea, and creatinine while renal tissues obtained were used for histopathological examinations. Results: Significantly higher values were seen in urea in the dichlorvos treated rats over a period of 24 hours, 14 days, and 30 days as compared to rats co-treated with palm oil and the control. Creatinine indicated significantly higher over a period of 24 hours while non-significant increases were observed in the dichlorvos treated rats over a period of 14 days and 30 days. More so, significantly higher values were seen in potassium in the dichlorvos treated rats over a period of 24 hours and 14 days, while significantly higher values in potassium were seen after period of 30 days as compared to rats co-treated with palm oil and the control. Sodium and chloride did not indicate significant difference over the period of 24 hours, 14 days, and 30 days. Histological examination of the renal tissue indicated structural distortions dichlorvos treated rats over a period of 24 hours, 14 days and 30 days while significant improvements in the structural integrity of the kidney were observed in rats co-treated with palm oil. Conclusion: Results obtained indicated that palm oil showed a protective effect in ameliorating the nephrotoxicity induced by dichlorvos as shown by the histological examination and decreased values of creatinine and urea as well as potassium in palm oil treated rats.

Sign in / Sign up

Export Citation Format

Share Document