Analysis of TNF-α and IL-4 Levels with Soil Transmitted Helminth Infection in Adults In Pasie Nan Tigo Village 2020

Intestinal helminth infections are caused by soil-transmitted helminths (STH). Worm infections are classified as neglected diseases, namely infections that are not noticed and are chronic diseases, rarely cause clear clinical symptoms and the impact is only seen in the long term. STH infection affects the immune response in humans in the form of increased levels of immunoglobulin E (IgE), eosinophilia and production of t-helper 2 (Th2), especially to changes in TNF-α and IL-4 levels. The purpose of this study was to determine the analysis of TNF-α and IL-4 levels with soil-transmitted helminths infection in adults. The research design used was case control, with a multistage-random sampling technique. Stool samples were taken from 127 adults and 24 subjects (18.9%) were infected with STH using the kato katz examination method. Furthermore, 24 STH-infected subjects and 24 healthy control subjects were taken using enzyme-linked immunosorbent assay (ELISA). The study population was male and female adults aged 20-60 years as many as 48 people. The data was carried out by independent t-test (p<0.05) and it was considered that there was a significant relationship. c. The results showed that there was a significant difference in TNF- levels in adults in the STH-infected group and healthy yangiene with p value = 0.001 and there was a significant difference in IL-4 levels in adults in the STH-infected group with p value = 0.001. The conclusion of this study is that there are differences in TNF- levels lower in STH-infected people than healthy controls and higher IL-4 in STH-infected people than healthy controls in adults.

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Alteration of Serum Levels of Cytokines in Schizophrenic Patients before and after Treatment with Risperidone

Iranian Journal of Allergy Asthma and Immunology ◽

10.18502/ijaai.v18i3.1119 ◽

2019 ◽

Cited By ~ 1

Author(s):

Esfandiar Azizi ◽

Ahmad Zavaran Hosseini ◽

Sara Soudi ◽

Ahmad Ali Noorbala

Keyword(s):

Healthy Subjects ◽

Serum Levels ◽

Enzyme Linked Immunosorbent Assay ◽

Healthy Controls ◽

Syndrome Scale ◽

Tnf Α ◽

Significant Difference ◽

Before And After ◽

Schizophrenic Patients ◽

Ifn Γ

A growing body of evidence suggests the existence of abnormalities in the immune system of schizophrenic patients. The current study examined serum levels of interleukin (IL) -1β, IL-6, IL-2,interferon(IFN) -γ, and tumor necrosis factor(TNF)-α in schizophrenic patients before and after treatment with risperidone and correlated levels of these cytokines with symptomatology. The study group consisted of 24 schizophrenic patients as defined by Diagnostic and Statistical Manual of Mental Disorders 4th edition (DSM-IV) criteria and 24 healthy controls. Serum cytokine levels were examined using enzyme-linked immunosorbent assay (ELISA). Schizophrenic symptomatology was assessed with the Positive and Negative Syndrome Scale (PANSS) questionnaire. The serum levels of TNF-α, IL-1β and IL-6 were significantly higher in participants before treatment compared with the healthy controls and after treatment (p<0.001). IFN-γ and IL-2 levels were significantly lower in participants after treatment compared with before treatment and the healthy controls (p<0.001). Except for IL-6 (p<0.05), there was no significant difference in the levels of TNF-α and IL-1β between the patients receiving treatment and the healthy subjects. Moreover, there was no significant difference in levels of IFN-γ and IL-2 between patients before treatment and the healthy subjects. There were no significant correlations between the concentration of cytokines studied and the PANSS. Positive intercorrelations between the production of IFN-γ and IL-2 were detected for sums of all groups(r=0.33, p=0.005). Clinical improvement of treated patients was associated with a reduction in the studied cytokines. It seems that changes in the cytokines level may play a significant role in the psychopathology of these patients.

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Pemberian Ekstrak Cacao (Theobroma Cacao L) Pada Model Huvecs Preeklampsia Terhadap Kadar Interleukin 8

Jurnal Kesehatan Prima ◽

10.32807/jkp.v13i2.236 ◽

2019 ◽

Vol 13 (2) ◽

pp. 93

Author(s):

Budi Rahayu ◽

Liberty Barokah

Keyword(s):

Multiple Comparisons ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

P Value ◽

Control Group Design ◽

Group Design ◽

Tnf Α ◽

Significant Difference ◽

Post Test ◽

Theobroma Cacao L

Preeklampsia dan eklampsia adalah penyakit pada kehamilan yang menjadi salah satu penyumbang angka kematian Ibu khususnya di Indonesia. Tahapan pertama dari preeklampsia ini adalah terjadi abnormalitas dalam remodelling vaskular trofoblastik mengakibatkan disfungsi endotel, tahap kedua pelepasan faktor plasenta kedalam sirkulasi darah seperti soluble fms-like tyrosine kinase (sFlt-1), TNF-α, IL-6, IL-8, angiotensin II tipe 1 reseptor antibodies (AT1-AA), dan thromboxan (TX) menyebabkan respon inflamasi dan peningkatan aktivasi sel endotel. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak kakao terhadap kadal IL-8 pada model HUVECs Preeklampsia. Penelitian true experimental dengan pendekatan yang digunakan post test only control group design yang dikerjakan dilaboratorium dengan invitro. Kultur HUVECs dibagi menjadi 5 kelompok masing-masing dilakukan replikasi 5x. Pengukuran kadar IL-8 menggunakan ELISA (Enzyme-Linked Immunosorbent Assay). Analisis data yang digunakan pada penelitian ini adalah dengan uji Anova one way pada kadar IL-8, selanjutnya dilakukan uji perbandingan berganda (Multiple Comparisons) dengan uji Beda Nyata Terkecil/BNT (Least Significant Difference/LSD) Hasil penelitian pemberian ekstrak kakao pada kelompok kontrol positif dan 3 dosis kelompok perlakuan ternyata dapat menurunkan secara bermakna (p-value 0.000< α 0.05, Anova One way) kadar IL-8.

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Relationship between Soil-Transmitted Helminths Infection and Insulin Sensitivity in Adults at Padang City

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v7i2.24117 ◽

2021 ◽

Vol 7 (2) ◽

pp. 84

Author(s):

Sitti Monica Astrilia Ambon ◽

Nuzulia Irawati ◽

Almurdi Almurdi

Keyword(s):

Insulin Sensitivity ◽

Metabolic Diseases ◽

Fasting Blood Glucose ◽

Sampling Technique ◽

Infected Group ◽

P Value ◽

Fasting Insulin ◽

Cross Sectional ◽

Soil Transmitted Helminths ◽

Significant Difference

Helminth infections in Indonesia are most often caused by Soil-transmitted Helminths (STH) species. It turns out that STH infection has a protective effect against metabolic diseases by affecting glucose metabolism and insulin sensitivity. The effect related to the immune response that caused by STH infection. The aim of this study was to investigate the relationship between STH infection with insulin sensitivity in adults at Padang City. This study design was a comparative cross-sectional study with multistage random sampling technique. Stool samples were taken from 127 adults and there were 24 subjects (18.9%) infected with STH. Furthermore, We took another 24 uninfected subjects and compared their fasting blood glucose (FBG) and fasting insulin. Then, from the data We calculated it’s Homeostatic Model for Insulin Resistance (HOMA-IR) score to assess insulin sensitivity. Unpaired T test and chi-square were used to analyze the data. The study result showed the average levels of FBG and fasting insulin were lower in STH-infected group than STH-uninfected group. However, there was no significant difference between two groups (FBG p=0.678; fasting insulin p=0.075). Also, STH-infected group tend to had good insulin sensitivity (66.7%) than STH-uninfected group (45.8%), but statistically, there was no significant relationship between STH infection and insulin sensitivity with p value=0.244. In conclusion, there is no relationship between STH infection and insulin sensitivity. Keywords: Soil-transmitted Helminths, Insulin Sensitivity, FBG

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Serum HMGB1 level is correlated with serum I-FABP level in neonatal patients with necrotizing enterocolitis

BMC Pediatrics ◽

10.1186/s12887-021-02818-6 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Ruyahan Huo ◽

Heng Liu ◽

Jing Chen ◽

Hong Sheng ◽

Li Miao

Keyword(s):

Necrotizing Enterocolitis ◽

Clinical Characteristics ◽

Serum Levels ◽

Stage Ii ◽

Inflammatory Factors ◽

Enzyme Linked Immunosorbent Assay ◽

Healthy Controls ◽

Hmgb1 Level ◽

Tnf Α ◽

Significant Difference

Abstract Background This study aims to investigate clinical significance of HMGB1 in neonatal patients with necrotizing enterocolitis (NEC). Methods This observational study enrolled a total of 106 stage II-III NEC neonatal patients, who were admitted in our hospital from March 2014 to March 2019. In addition, 99 suspected NEC patients and 200 healthy controls were included. The serum levels of HMGB1, I-FABP, and inflammatory factors CRP, IL-1β, IL-6 and TNF-α were determined by enzyme-linked immunosorbent assay (ELISA). Then, the demographic data and clinical characteristics of all patients were collected. Statistical analysis was conducted to determine the correlation between HMGB1 and the clinical characteristics. Results No significant difference was found in the basic characteristics of NEC patients and healthy controls, except for birth weight and gestational age. The expression levels of HMGB1, I-FABP, and inflammatory factors IL-1β, IL-6 and TNF-α were significantly higher in NEC patients, when compared to healthy controls. The serum levels of HMGB1, I-FABP, IL-1β and IL-6 markedly increased in stage II-III NEC patients, when compared to stage I NEC patients. The Pearson’s analysis revealed a positive correlation between HMGB1 and I-FABP, HMGB1 and IL-1β, and HMGB1 and IL-6. The ROC curve revealed that both HMGB1 and I-FABP can potentially be used as diagnostic factors for NEC. The logistic multivariate regression revealed that I-FABP, IL-1β and IL-6 are independent risk factors for mortality in neonatal NEC patients. Conclusions Serum HMGB1 levels are upregulated in neonatal NEC patients, and these are correlated with the patient’s prognosis.

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Over-expression of IL-6 coding gene in the peripheral blood of migraine with aura patients

Human Antibodies ◽

10.3233/hab-210442 ◽

2021 ◽

pp. 1-5

Author(s):

Mahdi Ramezani ◽

Alireza Komaki ◽

Mohammad Mahdi Eftekharian ◽

Mehrdokht Mazdeh ◽

Soudeh Ghafouri-Fard

Keyword(s):

Migraine With Aura ◽

Peripheral Blood ◽

Healthy Subjects ◽

Migraine Without Aura ◽

P Value ◽

Healthy Controls ◽

Over Expression ◽

Significant Difference ◽

Male Patients ◽

Years Lived With Disability

Migraine is a common disorder which is placed among the top ten reasons of years lived with disability. Cytokines are among the molecules that contribute in the pathophysiology of migraine. In the current study, we evaluated expression levels of IL-6 coding gene in the peripheral blood of 120 migraine patients (54 migraine without aura and 66 migraine with aura patients) and 40 healthy subjects. No significant difference was detected in expression of IL-6 between total migraine patients and healthy controls (Posterior beta = 0.253, P value = 0.199). The interaction effect between gender and group was significant (Posterior beta =-1.274, P value = 0.011), therefore, we conducted subgroup analysis within gender group. Such analysis revealed that while expression of this gene is not different between male patients and male controls (Posterior beta =-0.371, P value > 0.999), it was significantly over-expressed in female patients compared with female controls (Posterior beta = 0.86, P= 0.002). Expression of IL-6 was significantly higher in patients with aura compared with controls (Posterior beta = 0.63, adjusted P value = 0.019). However, expression of this cytokine coding gene was not different between patients without aura and healthy subjects (Posterior beta = 0.193, adjusted P value = 0.281). Therefore, IL-6 might be involved in the pathophysiology of migraine among females and migraine with aura among both sexes.

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POS1290 CYTOKINE BIOMARKERS IN COMMON LOW BACK PAIN

Annals of the Rheumatic Diseases ◽

10.1136/annrheumdis-2021-eular.4205 ◽

2021 ◽

Vol 80 (Suppl 1) ◽

pp. 926.3-926

Author(s):

R. Dhahri ◽

A. Dghaies ◽

M. Slouma ◽

L. Metoui ◽

I. Gharsallah ◽

...

Keyword(s):

Neuropathic Pain ◽

Low Back Pain ◽

Back Pain ◽

Inflammatory Cytokines ◽

Enzyme Linked Immunosorbent Assay ◽

Healthy Controls ◽

Low Back ◽

Tnf Α ◽

Functional Scores ◽

The Mean

Background:Common low back pain (LBP) is a common health problem affecting 50 to 80% of working age adults. It is one of the common and costly health problems in Tunisia. Actually, the role of the immune response and inflammatory cytokines in the pathogenesis of chronic pain has been of growing interest.Objectives:The aim of this study was to assess whether pro and anti-inflammatory cytokines could be detected in serum in patients with LBP compared with healthy subjects and whether they could be related to pain severity and to clinical findings.Methods:It was a an analytical cross-sectional study including 50 patients with at least three months of LBP, in the department of rheumatology, orthopedics and immunology at the Military Hospital of Tunis between January 1st and March 31, 2020. All patients had a standardized clinical assessment.Levels of serum cytokines IL-6, IL-8, IL-1β and TNF- α, were measured using the chimiluminescence technique. Serum concentration of IL-10 was assayed by the enzyme-linked immunosorbent assay technique (ELISA). The normal levels of cytokines were determined in 50 healthy controls.Results:The mean age of the patients was 41.9 ± 8.4 years and the sex ratio was 4.5. LBP duration was 66.4 months. The mean lumbar visual analog scale (VAS) was 4.5 ± 1.9, and the root VAS was 2.6 ± 2.5. Neuropathic pain was found in 26% of patients. The average BMI was 27 ± 3.7 kg/m2. Only serum level of IL-8 was significantly higher in subjects with LBP compared to healthy controls (p <10-3). IL-1β was indetectable in both patients and controls. Positive correlations were found between IL-8 levels and anxiety/functional scores (r = 0.3; p = 0.02/ r = 0.3; p = 0.04). IL-6 was positively correlated with BMI, and negatively correlated with the Schober test. No correlations were found between serum levels of IL-6, IL-8, IL-10, TNF-α and pain intensity (VAS), neuropathic pain (DN4), fibromyalgia (FIRST), depression (HAD) and various radiological data.Conclusion:Interleukin-8 is a biomarker of common low back pain and correlate with anxiety and functional disability. These results suggest that IL-8 may be a therapeutic target to reduce chronic back pain and reduce the social and profession impact.Disclosure of Interests:None declared

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Elevated IL-6 and IL-1β are associated with temporal lobe epilepsy: A study in Chinese patients

European Journal of Inflammation ◽

10.1177/2058739218778934 ◽

2018 ◽

Vol 16 ◽

pp. 205873921877893 ◽

Cited By ~ 1

Author(s):

Li Xia ◽

Song-Qing Pan ◽

Qiu-Min Zhang ◽

Qin Zhou ◽

Lu Xia ◽

...

Keyword(s):

Temporal Lobe Epilepsy ◽

Temporal Lobe ◽

Chinese Patients ◽

Enzyme Linked Immunosorbent Assay ◽

Healthy Controls ◽

Temporal Epilepsy ◽

Tnf Α ◽

Plasma Cytokines ◽

Proinflammatory Molecules ◽

Positive Correlations

Activation of proinflammatory cytokines in seizures has been well characterized. However, role of cytokines in epilepsy and association with different clinical phenotype has not been well investigated. Reports on possible link between proinflammatory molecules and epilepsy are very limited. In this study, we performed a hospital-based case control study to investigate the association of plasma cytokines and their expression with different clinical categories of epilepsy. Patients admitted to Neurology Department of Renmin Hospital were enrolled in this study after clinical investigations. In all, 92 patients with temporal lobe epilepsy (TLE) and 45 with extra-temporal lobe epilepsy (XTLE) were included in this study. Furthermore, we included 86 healthy controls from the similar geographical population. Plasma levels of interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β were quantified by enzyme-linked immunosorbent assay (ELISA). All plasma cytokines were elevated in TLE and XTLE compared to healthy controls ( P < 0.0001). Furthermore, IL-6 and IL-1β were significantly higher in TLE when compared to extra-temporal epilepsy. Incidentally, no difference in mean plasma TNF-α levels was noticed among TLE and XTLE. Positive correlations were observed between all plasma proinflammatory molecules (TNF-α, IL-6, and IL-1β) investigated in this study. Epilepsy patients displayed higher proinflammatory molecules, namely, IL-6, IL-1β, and TNF-α. Plasma IL-6 and IL-1β can be use as biomarkers for differentiation of TLE from XTLE.

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IL-32 Serum Levels in Coronary Artery Disease Patient and its Relationship with IL-6 and TNF-α Serum Levels

10.21203/rs.3.rs-179059/v1 ◽

2021 ◽

Author(s):

Mina Mohammad-Rezaei ◽

Reza Ahmadi ◽

Ali Rafiei ◽

Arsalan Khaledifar ◽

Shohila Fatahi ◽

...

Keyword(s):

Coronary Artery Disease ◽

Coronary Artery ◽

Serum Levels ◽

Arterial Stenosis ◽

Enzyme Linked Immunosorbent Assay ◽

Control Subjects ◽

Tnf Α ◽

Significant Difference ◽

Major Vessel ◽

Artery Disease

Abstract Coronary Artery Disease (CAD) is a chronic inflammatory disease caused by atherosclerosis and arteries become clogged due to plaque formation, fat accumulation, and various sorts of immune cells. IL-32 is a new proinflammatory cytokine, which enhances inflammation through inducing different inflammatory cytokines. The purpose of current research was to assess IL-32 serum levels in coronary artery disease subjects and its relationship with serum levels of IL-6 and TNF-α. Forty-two subjects diagnosed with CAD and thirty-nine control subjects were enrolled in the research. Serum levels of IL-6, TNF-α, and IL-32 were measured using the enzyme-linked immunosorbent assay (ELISA). IL-32, TNF-α, and IL-6 serum levels were significantly higher by 2.7, 3.48, and 3.2-fold in the CAD subjects than in control subjects, respectively. Moreover, no significant difference was found in TNF-α, IL-6 and IL-32 serum levels with the clogged arteries number in the CAD group. TNF-α and IL-32 serum levels in the CAD subjects with cardiac arterial stenosis in one major vessel were significantly increased than CAD subjects with cardiac arterial stenosis in more than one major vessels. ROC curve analysis revealed that serum levels of IL-32, TNF-α, and IL-6 showed good abilities in predicting CAD. Also, Multiple logistic regression analyses suggested that TNF-α, IL-6, and IL-32, serum levels of LDL and ox-LDL were independently related to the presence of CAD, while HDL serum levels were not. TNF-α, IL-32, and IL-6 showed an increase in CAD group and serum levels of these cytokines showed good abilities in predicting CAD. Our data suggested the involvement of TNF-α and IL-32 in the early stage of CAD.

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Anti-phosphatidylserine/prothrombin Complex Antibodies in Patients With Cutaneous Vasculitis: Possible Involvement in the Pathogenesis

10.21203/rs.3.rs-120536/v1 ◽

2020 ◽

Author(s):

Yuto Tamura ◽

Tamihiro Kawakami ◽

Yupeng Dong ◽

Miku Yoshinari ◽

Yuka Nishibata ◽

...

Keyword(s):

Vascular Endothelium ◽

Systemic Vasculitis ◽

Serum Levels ◽

Cutaneous Vasculitis ◽

Enzyme Linked Immunosorbent Assay ◽

Healthy Controls ◽

Iga Vasculitis ◽

Skin Involvement ◽

Wild Type ◽

Significant Difference

Abstract Objective. It was previously demonstrated that cutaneous vasculitis, including IgA vasculitis and cutaneous arteritis (CA), is associated with the presence of IgM antibodies (Abs) against the phosphatidylserine/prothrombin complex (PS/PT). Recently, novel enzyme-linked immunosorbent assay kits for the detection of IgG and IgM anti-PS/PT (aPS/PT) Abs have become commercially available.Methods. The prevalence of serum IgG and IgM aPS/PT Abs in both cutaneous and systemic vasculitis was determined using these kits. In addition, to examine whether aPS/PT Abs were involved in the pathogenesis of cutaneous vasculitis, inbred wild-type rats were intravenously administered with a rat IgM class aPS/PT monoclonal Ab established previously or with rat immunoglobulins as controls. To express PS on the surface of vascular endothelium, these rats were given a subcutaneous injection of cell-free histones (250 µg/ml, 300 µl/site) 2 hours in advance. Results. Serum IgM aPS/PT Ab levels were elevated in patients with systemic vasculitis with skin involvement and CA compared to those in patients with systemic vasculitis without skin involvement and healthy controls. There was no significant difference in the serum levels of IgG aPS/PT Abs between the patients and healthy controls. Correspondingly, inbred wild-type rats intravenously administered with the aPS/PT monoclonal IgM Ab after appropriate priming—subcutaneous histone injection—developed cutaneous vasculitis. Some rats given rat IgM instead of the aPS/PT monoclonal Ab also developed cutaneous vasculitis, whereas vasculitis did not occur in rats given IgG or only priming by histones. Conclusion. IgM aPS/PT Abs could be involved in the pathogenesis of cutaneous vasculitis.

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The expression and significance of inflammatory cytokines and MMP-9 in the tears of the infected eye and contralateral uninfected eye in patients with fungal keratitis

10.21203/rs.3.rs-269176/v1 ◽

2021 ◽

Author(s):

Shuang Zhang ◽

Hui-Min Wu ◽

Xiang-Ni Cao ◽

Xian-Qi Zhang ◽

Gui-ping Gao

Keyword(s):

Tear Film ◽

Fungal Keratitis ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Healthy Controls ◽

Tnf Α ◽

Cytokine Levels ◽

Healthy Control ◽

The Relationship ◽

Tear Film Function

Abstract Background: We investigated bilateral tear cytokine levels including interleukin (IL)-1β, IL-10, IL-17, tumor necrosis factor (TNF)-α and Matrix metalloproteinase-9 (MMP-9) in patients with fungal keratitis(FK). Meanwhile, we evaluated the relationship between the changes of tear cytokines with corneal perception and pain in infected eyes, and the relationship between tear cytokines and tear film function in contralateral uninfected eyes .Methods : A total of 60(20 FK, 20 contralateral, 20 healthy controls) tear samples were collected prospectively and analyzed by enzyme linked immunosorbent assay(ELISA). Approximately 50 to 60 ul of tear samples in each case were collected. Meanwhile ,we analyzed the changes of visual analogue scale(VAS), tear breakup time (TBUT), Schirmer I test (SIT) and corneal perception compared with healthy controls. Results :The concentrations of IL-1β, IL-10 and IL-17 increased in bilateral eyes compared with healthy controls(P<0.05). The tear concentrations of MMP-9 , TNF-α only significantly increased in affected eyes (P <0.05). Patients with FK showed significant reduction in corneal perception of infected eyes compared with controls(P<0.05). Corneal perception of the normal eyes in FK patients was slightly lower than that of control group, but there was not statistical difference (P>0.05).TBUT and SIT of contralateral uninfected eyes were significantly lower than that of control group(P<0.05), which were significantly correlated with levels of IL-1β, IL-17(P<0.05). SIT were also negatively correlated with MMP-9(P<0.05), while the levels of IL-1β, IL-10, IL-17, TNF-α and MMP-9 in the tears of the healthy control group had no significant correlation with TBUT and SIT indicators(P>0.05).The corneal perception and VAS score of the affected FK eyes showed correlation with IL-1β, IL-17 and TNF-α(P<0.05).In addition, concentration of IL-10 inversely was correlated with VAS (P<0.05). Conclusion: Proinflammatory tear cytokines are elevated in bilateral eyes with unilateral FK as associated with tear film function ,pain and corneal sensitivity.

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