scholarly journals SSR and SNP genetic profiling of Armenian grape cultivars gives insights into their identity and pedigree relationships

OENO One ◽  
2021 ◽  
Vol 55 (4) ◽  
pp. 101-114
Author(s):  
Anna Nebish ◽  
Javier Tello ◽  
Yolanda Ferradás ◽  
Rouben Aroutiounian ◽  
José Miguel Martínez-Zapater ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Agricultural Sector ◽  
Genetic Identification ◽  
Vitis Vinifera L ◽  
Correct Identification ◽  
South Caucasus ◽  
Genetic Profiling ◽  
Genetic Pool ◽  
First Time ◽  
International Academy

The South Caucasus is recognised as the primary Vitis vinifera L. (grapevine) domestication centre and has a high diversity of wild and cultivated grapevines. Archaeological findings indicate that winemaking activities have existed in Armenia for more than 6,000 years, viticulture being one of the most important activities of the modern Armenian agricultural sector. Despite this relevance, some grapevines in local collections have not yet been properly identified, thus hindering the efficient conservation, characterisation and eventual use of autochthonous genetic resources. In the present study, a combined SNP and SSR profiling strategy was used for the genetic identification of a series of grapevine accessions from the Grape Collection of the International Academy of Viticulture and Winemaking in Nalbandyan, presumed to be autochthonous Armenian varieties. The results provided useful information for the correct identification of these genetic resources, revealing multiple cases of synonyms, homonyms and misnames. The genetic data made it possible to confirm the pedigree proposed for some of the cultivars identified in this study and to clarify the origin of others. In addition, we propose, for the first time, a series of new trios and duos involving autochthonous Armenian grapevines. The singularity of this genetic pool compared to other Western and Central European varieties, as well as the potential novel sources of variability in traits of interest (e.g., seedlessness) that were found, highlight the importance of improving knowledge of the Armenian grapevine genetic pool.

scholarly journals Givira ethela (Neumoegen and Dyar, 1893) (Lepidoptera: Cossidae), A Previously Unidentified Pest on Vitis vinifera (L.)

Insects ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 239
Author(s):  
Davide Scaccini ◽  
Enrico Ruzzier ◽  
Kent M. Daane
Keyword(s):  
Vitis Vinifera ◽  
Natural Enemies ◽  
Management Practices ◽  
Agricultural Sector ◽  
Central Valley ◽  
Vitis Vinifera L ◽  
Correct Identification ◽  
Pest Species ◽  
Monitoring Methods ◽  
Management Options

Grape cultivation is a billion-dollar agricultural sector in California, where invasive or novel pest species can disrupt management practices. We report herein on a new pest associated with California vineyards, the carpentermoth Givira ethela (Neumoegen and Dyar, 1893). Rather than an invasive species, G. ethela appears to be a newly recognized wood-boring pest of Vitis vinifera (L.) in regions of California’s Central Valley, where its initial occurrence has been dated back to, at least, the beginning of the 2000s. The habitus of adult, genitalia and pupa is illustrated. Givira ethela distribution in California is updated including published records and new data. Carpentermoth galleries seem to facilitate the access of Planococcus ficus Signoret, 1875 to vine sap and protection from natural enemies, environmental stresses, and pesticide treatments. Notes on pest status, life history, monitoring practices, natural enemies, and management options on grapes are also discussed. Tools for the Integrated Pest Management of G. ethela should include the correct identification of the insect and its damage, a full understanding of its biology and ecology, the application of monitoring methods, and the identification of economic thresholds and injury levels.

scholarly journals Unknown grapevine genetic resources in Asturias (Spain) on the edge of extinction

OENO One ◽  
2013 ◽  
Vol 47 (4) ◽  
pp. 249
Author(s):  
Paula Moreno-Sanz ◽  
María Dolores Loureiro ◽  
Belén Suárez
Keyword(s):  
Genetic Resources ◽  
Complete Characterization ◽  
Genetic Identification ◽  
Model Description ◽  
Northern Spain ◽  
Table Grapes ◽  
Varietal Identification ◽  
Breeding Programmes ◽  
First Time

<p style="text-align: justify;"><strong>Aim</strong>: Grapevine is a traditional crop in Asturias (northern Spain). However, an important part of the varietal heritage of this region is still unknown. Great biodiversity was reported in previous surveys, with varietal identification being carried out via microsatellite markers. The aim of the present study was to confirm the previous genetic identification and provide the first description of the unknown cultivars.</p><p style="text-align: justify;"><strong>Methods and results</strong>: Forty cultivars were described <em>in situ</em> over a two-year period through 58 ampelographic descriptors. A model description was performed for each cultivar and great variability was found : white, red and rosé grape cultivars ; hybrids ; winemaking and dual-use (wine and table grapes) cultivars ; and some teinturier cultivars. Cluster analysis grouped cultivars with similar characteristics.</p><p style="text-align: justify;"><strong>Conclusion</strong>: Ampelographic description was complementary to molecular identification in confirming the identity of the studied cultivars. Moreover, unknown cultivars have been described for the first time. Complete characterization is necessary because these cultivars constitute valuable genetic resources for crop breeding programmes.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: Considering the limited number of accessions of the unknown cultivars and the replanting of old vineyards with vines approved by <em>Cangas Quality Wine</em> regulations, their conservation and study is vital to provide genetic resources for potential breeding or technological purposes in the future.</p>

scholarly journals Identification and Characterization of New ‘Candidatus Phytoplasma solani’ Strains Associated with Bois Noir Disease in Vitis vinifera L. Cultivars Showing a Range of Symptom Severity in Georgia, the Caucasus Region

Plant Disease ◽  
2016 ◽  
Vol 100 (5) ◽  
pp. 904-915 ◽  
Author(s):  
Fabio Quaglino ◽  
David Maghradze ◽  
Paola Casati ◽  
Nona Chkhaidze ◽  
Mzagho Lobjanidze ◽  
...  
Keyword(s):  
Symptom Severity ◽  
Phylogenetic Analyses ◽  
Vitis Vinifera L ◽  
Polymorphism Analysis ◽  
South Caucasus ◽  
Bois Noir ◽  
Caucasus Region ◽  
Symptom Intensity ◽  
First Time

Evidence from a preliminary survey highlighted that ‘Candidatus Phytoplasma solani’, the etiological agent of bois noir (BN) disease of grapevine, infects grapevine varieties in Georgia, a country of the South Caucasus. In this study, field surveys were carried out to investigate the BN symptom severity in international and Georgian native varieties. ‘Ca. P. solani’ was detected and identified by polymerase chain reaction-based amplification and restriction fragment length polymorphism analysis of 16S ribosomal DNA, and further characterized by multiple gene typing analysis (vmp1 and stamp genes). Obtained data highlighted that the majority of Georgian grapevine varieties showed moderate and mild symptoms, whereas international cultivars exhibited severe symptoms. Molecular characterization of ‘Ca. P. solani’ from grapevine revealed the presence of 11 distinct phytoplasma types. Only one type (VmGe12/StGe7) was identical to a strain previously reported in periwinkle from Lebanon; the other ‘Ca. P. solani’ types are described here for the first time. Phylogenetic analyses of vmp1 and stamp gene concatenated nucleotide sequences showed that ‘Ca. P. solani’ strains in Georgia are associated mainly with the bindweed-related BN host system. Moreover, the fact that ‘Ca. P. solani’ strains are distributed in grapevine cultivars showing a range of symptom intensity suggests a different susceptibility of such local cultivars to BN.

Implication of the distinctive bipolar intracardiac electrograms for ventricular arrhythmias arising from different regions of ventricular outflow tract

EP Europace ◽  
2020 ◽  
Vol 22 (9) ◽  
pp. 1367-1375
Author(s):  
Jia Li ◽  
Weiqian Lin ◽  
Cheng Zheng ◽  
Chi Zhang ◽  
Jiji Yu ◽  
...  
Keyword(s):  
Ventricular Arrhythmias ◽  
Ventricular Outflow Tract ◽  
High Amplitude ◽  
Outflow Tract ◽  
Correct Identification ◽  
Aortic Sinus ◽  
Distinctive Features ◽  
Long Duration ◽  
Target Sites ◽  
First Time

Abstract Aims To investigate the characteristics of bipolar intracardiac electrograms (bi-EGMs) in target sites of ventricular arrhythmias (VAs) originating from different regions of ventricular outflow tract (VOT). Methods and results Two hundred and seventy patients undergoing first-time ablation for VAs originated from distal great cardiac vein (DGCV), aortic sinus cusps (ASCs), or pulmonary sinus cusps (PSCs) were enrolled in present study. Local intracardiac bipolar recordings on 243 successful sites and 506 attempted but unsuccessful ablation sites were analysed. Specific potentials in bi-EGMs on successful sites were more common compared with unsuccessful sites (76.95%, 187/243 vs. 25.49%, 129/506, P &lt; 0.05). A total of 60.00% (81/135) patients in ASCs group presented a presystolic short-duration fractionated potential, higher than 23.21% (13/56) in DGCV and 23.08% (12/52) in PSCs (all P &lt; 0.05); 44.23% (23/52) patients in PSC group showed a presystolic high-amplitude discrete potential, while 1.79% (1/56) in DGCV and 2.22% (3/135) in ASCs (all P &lt; 0.05); 41.07% (23/56) patients in DGCV group showed bi-EGMs of presystolic long-duration multicomponent fractionated potential, which was significantly higher than 3.85% (2/52) in PSCs and 4.44%(6/135) in ASCs (all P &lt; 0.05). Conclusion Distinctive morphology of bi-EGMs during VAs can be found in different regions of VOT, which probably due to changes in the arrangements of myocardial sleeves. Correct identification and better understanding of the distinctive features of these bi-EGMs with regards to the anatomic location was important, the presence of specific potentials may add help in successful ablation.

scholarly journals Methodological Approaches to DNA Authentication of Foods, Wines and Raw Materials for Their Production

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 595
Author(s):  
Aram G. Galstyan ◽  
Vladislav K. Semipyatniy ◽  
Irina Yu. Mikhailova ◽  
Khamid Kh. Gilmanov ◽  
Alana V. Bigaeva ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Dna Extraction ◽  
Raw Materials ◽  
Direct Sequencing ◽  
Genetic Identification ◽  
Proteinase K ◽  
Vitis Vinifera L ◽  
Acid Extraction ◽  
Plant Component ◽  
Sensitivity Specificity

DNA authentication of wines is a process of verifying their authenticity by genetic identification of the main plant component. The sample preparation of experimental and commercial wines was carried out by precipitation of wine debris by centrifugation with preliminary exposure with precipitators and co-precipitators, including developed macro- and micro- volume methods applicable to white or red wines, using polyvinylpyrrolidone as a co-precipitator. Addition of 2-mercaptoethanol and proteinase K to the lysing solution made it possible to adapt the technology for DNA extraction from the precipitated wine debris. The additionally tested technique of DNA extraction from wine debris by dimethyl sulfoxide (DMSO) lysis had fewer stages and, consequently, a lower risk of contamination. The results of further testing of one of the designed primer pairs (UFGT-F1 and UFGT-R1) in conjunction with the tested methods of wine material sample preparation and nucleic acid extraction, showed the advantage in the given set of oligonucleotides over previously used ones in terms of sensitivity, specificity and reproducibility. The developing strategy for genetic identification of grape varieties and DNA authentication of wines produced from them based on direct sequencing of polymerase chain reaction (PCR) products is implemented by interpreting the detected polymorphic positions of variable Vitis vinifera L. UFGT gene locus with distribution and split into 13 UFGT gene-associated groups.

scholarly journals Burkholderia species in human infections in Mexico: Identification of B. cepacia, B. contaminans, B. multivorans, B. vietnamiensis,B. pseudomallei and a new Burkholderia species

2021 ◽  
Vol 15 (6) ◽  
pp. e0009541
Author(s):  
Georgina Meza-Radilla ◽  
Violeta Larios-Serrato ◽  
Rigoberto Hernández-Castro ◽  
J. Antonio Ibarra ◽  
Paulina Estrada-de los Santos
Keyword(s):  
Sensu Stricto ◽  
Clinical Settings ◽  
Correct Identification ◽  
Opportunistic Pathogens ◽  
Burkholderia Species ◽  
Medical Centers ◽  
Accurate Treatment ◽  
Immunosuppressed Patients ◽  
Human Infections ◽  
First Time

Background Burkholderia sensu stricto is comprised mainly of opportunistic pathogens. This group is widely distributed in the environment but is especially important in clinical settings. In Mexico, few species have been correctly identified among patients, most often B. cepacia is described. Methodology/Principal findings In this study, approximately 90 strains identified as B. cepacia with the VITEK2 system were isolated from two medical centers in Mexico City and analyzed by MLSA, BOX-PCR and genome analysis. The initial identification of B. cepacia was confirmed for many strains, but B. contaminans, B. multivorans and B. vietnamiensis were also identified among clinical strains for the first time in hospitals in Mexico. Additionally, the presence of B. pseudomallei was confirmed, and a novel species within the B. cepacia complex was documented. Several strains misidentified as B. cepacia actually belong to the genera Pseudomonas, Stenotrophomonas and Providencia. Conclusions/Significance The presence of different Burkholderia species in Mexico was confirmed. Correct identification of Burkholderia species is important to provide accurate treatment for immunosuppressed patients.

scholarly journals Xerodrymionorientale mediterraneum gorges of the Karatag river

2021 ◽  
Vol 38 ◽  
pp. 00022
Author(s):  
Sayfiddin Khairiddin Davlatov ◽  
Rakhmatullo Boboevich Sattorov ◽  
Jamoliddin Murotalievich Bobokalonov
Keyword(s):  
Characteristic Feature ◽  
High Altitude ◽  
Genetic Resources ◽  
Plant Species ◽  
Field Research ◽  
The State ◽  
Pistacia Vera ◽  
Wild Fruit ◽  
Middle Mountains ◽  
First Time

The Karatag gorge is one of the natural areas where valuable genetic resources (wild, fruit and many valuable species) are preserved. A characteristic feature of the region is the richness of the diversity of flora and vegetation, where the main formations of Tajik vegetation are noted (maple, hazel, almond, frame, juniper). The article summarizes the results of the authors’ field research on the study of the state of xerophilic forests in the Karatag gorge. For the first time, the authors cite original materials on the phytocenology of all formations of this type of composition. According to the results of our research, the Shibleak communities in the study area are distributed in high-altitude belts from low-hilly 600–800 m to middle mountains 800–1800, 2000 m. The main formations of this type in the study area are: Acer regelii, Crataegus pontica, Celtis caucasic, Pistacia vera, Amygdalis bucharica, Ampelopsis vitifolia, Atraphaxis pyrifolia. As a result of the study, 340 plant species, 6 formations and more than 25 vegetation associations were identified in the composition of the flora of this type of the study area.

scholarly journals Genetic identification of the yeast Saccharomyces kudriavzevii from European population

2009 ◽  
Vol 7 (1) ◽  
pp. 9-11
Author(s):  
Gennadiy I Naumov
Keyword(s):  
Biological Species ◽  
European Population ◽  
Hybridization Analysis ◽  
Genetic Identification ◽  
Saccharomyces Kudriavzevii ◽  
First Time

Using genetic hybridization analysis we showed for the first time that Portuguese isolates belong to the biological species Saccharomyces kudriavzevii Naumov et al. (2000). Earlierthis species was described on Japanese isolates. Divergence of Portuguese and Japanese S. kudriavzevii populations, as well as different S. cerevisiae populations, on molecular galactose markers is discussed.

scholarly journals Genetic Relationship and Diversity in Some Ornamental Palms Based on Proteins and Randomly Amplified Polymorphic DNA Markers

HortScience ◽  
2017 ◽  
Vol 52 (3) ◽  
pp. 338-342
Author(s):  
Antar Nasr El-Banna ◽  
Mohammed Elsayed El-Mahrouk ◽  
Mohammed Eraky El-Denary ◽  
Yaser Hassan Dewir ◽  
Yougasphree Naidoo
Keyword(s):  
Genetic Variation ◽  
Electrophoretic Pattern ◽  
Genetic Identification ◽  
Randomly Amplified Polymorphic Dna ◽  
Protein Markers ◽  
Conservation Genetic ◽  
Average Similarity ◽  
Molecular Sizes ◽  
High Level ◽  
First Time

For the first time, genetic diversity among 14 ornamental palm accessions originating from different countries and grown in different regions in Egypt were examined. Identification of genetic variation and phylogenetic relationships in ornamental palms would be useful for its genetic identification, improvement, and conservation. Genetic polymorphism was analyzed using the randomly amplified polymorphic DNA (RAPD) as well as protein markers. The electrophoretic pattern of protein analysis produced 21 bands distributed in all accessions with molecular sizes ranging from 11.8 to 99.3 KDa. Some accessions possessed some bands, which were absent in other accessions and could be used for their identification. Furthermore, 10 RAPD selected primers were employed to determine genetic variation among the 14 palm genotypes as well as to test the effectiveness of RAPD primers as a genetic marker. RAPD analysis revealed a high level of polymorphism (100%) among the studied accessions. A total number of 310 amplified bands were generated across the studied genotypes with an average of 30 bands per primer. Cluster analysis using sequence alignment was done to generate a dendrogram verifying the relationship among the 14 studied ornamental palms, with an average similarity matrix range of 0.00 to 0.08 and 0.39 to 0.93 for RAPD and protein markers, respectively. It is concluded that, both SDS-protein and RAPD markers are equally important for genetic analysis and are suitable for the characterization of ornamental palm collection.

scholarly journals Fruit Spirit Production from Coffee Cherries—Process Analysis and Sensory Evaluation

Beverages ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 57
Author(s):  
Daniel Einfalt ◽  
Klaus Meissner ◽  
Lena Kurz ◽  
Kiatkamjon Intani ◽  
Joachim Müller
Keyword(s):  
Sensory Evaluation ◽  
Agricultural Sector ◽  
Ethanol Yield ◽  
Process Analysis ◽  
Total Yield ◽  
Fruit Production ◽  
Sensorial Attributes ◽  
Distillate Fractions ◽  
First Time ◽  
Fruit Spirits

Coffee fruit production is an important agricultural sector in more than 70 tropical countries. However, the production of fruit spirits based on coffee fruits has not been investigated to date. This study evaluated, for the first time, its fermentation and distillation performance, ethanol yield and sensorial attributes. A selected yeast strain (Saccharomyces cerevisiae L.) fermented coffee cherry mash within five days and produced ethanol concentrations of 31.0 g/L. The mash was distilled and distillate fractions were categorized for heads/hearts/tails by sensory evaluation, resulting in an ethanol mass ratio of 1.0:4.2:0.8 with a total yield of 1.8% (w/w) ethanol based on coffee cherry mash. Analysis of fermentative volatiles indicated comparatively high methanol contents of 26 ± 4 g/L ethanol in the hearts fraction. Sensory evaluation of the hearts fraction resulted in 15 spirit specific descriptors, with vegetal and nutty indicating the most important terms to describe the perception of coffee cherry spirit. The results suggested that there is a high potential to introduce a fruit spirit based on coffee fruits.

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