scholarly journals Digestibility Value and Fermentation Level of Local Feed-Based ration for Sheep

2019 ◽  
Vol 20 (2) ◽  
pp. 95
Author(s):  
Hamdi Mayulu ◽  
N Fauziah ◽  
M Christiyanto ◽  
Sunarso Sunarso ◽  
M I Haris
Keyword(s):  
Animal Feed ◽  
Animal Husbandry ◽  
Important Indicator ◽  
Dry Matter Digestibility ◽  
Feed Digestibility ◽  
Randomized Design ◽  
Vitro Analysis ◽  
Range Test ◽  
In Vitro Analysis

Feed Digestibility is an important indicator which can be used as a guideline to determine the amount of nutrient and feed that can be absorbed by digestive tract. The objective of the research was to determine the value dry matter digestibility (DMD), organic matter digestibility (OMD), NH3 fermentation level and volatile fatty acid (VFA) fermentation level. The research was conducted on January 2018 in Animal Feed and Nutrition Laboratory of Animal Husbandry and Agriculture Faculty, Diponegoro University Semarang. The research method used was in vitro analysis with complete randomized design with five treatments and four replicates. The data were analyzed with analysis of variance at 95% significant level, followed by Duncan Multiple Range Test. The results showed that the highest score of DMD was T5=56.51±0.33%, T4=56.41±2.11; T3=55.86±0.83; highest score of OMD was T5=62.48±0.39%, T4=61.87±1.60; T3=60.86±1.10, the highest NH3 concentration was T3=6.07±1.55 mM, and the highest VFA concentration was T5=55.25±3.40 mM. Sheep’s local feed-based ration was significantly (P<0.05) influenced in vitro DMD, OMD, NH3 fermentation level and VFA fermentation level

scholarly journals Nutrient content evaluation of dried poultry waste urea molasses block (DPW-UMB) on In-vitro analysis

2018 ◽  
Vol 16 (2) ◽  
pp. 50
Author(s):  
Danung Nur Adli ◽  
Osfar Sjofjan
Keyword(s):  
Crude Protein ◽  
Nutrient Content ◽  
Poultry Waste ◽  
Duncan Multiple Range Test ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Vitro Analysis ◽  
Range Test ◽  
In Vitro Analysis

<p><strong><em>The research purpose was to determine nutrient content of dried of poultry waste urea molasses block (DPW-UMB). The research method was used completely randomized design with 3 treatments and 5 replicates. The treatments used for research were T1 (10% manure of laying chicken and 25% molasses), T2 (15% manure of laying chicken and 30% molasses), and T3 (20% manure of laying chicken and 30% molasses). The data analysis was the analysis of variance (anova) and continued by Duncan Multiple Range Test. The results showed that treatments has significantly difference (P&lt;0.01) on dry matter, crude protein, and ash. It could be concluded that dpw-umb contained sufficient levels of nutrients. it could be used as feedstuff for ruminants for supplementation with the required nutrients.</em></strong></p>

scholarly journals In vitro analysis, an accurate tool to estimate dry matter digestibility in rabbits. Intra- and inter- laboratory variability

2010 ◽  
Vol 16 (4) ◽  
Author(s):  
  Carabaño R. ◽  
Nicodemus N. ◽  
García J. ◽  
Xiccato G. ◽  
Trocino A. ◽  
...  
Keyword(s):  
Dry Matter ◽  
Dry Matter Digestibility ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Pemberian Kulit Biji Kopi dalam Ransum Sapi Aceh Terhadap Kecernaan secara In Vitro

2013 ◽  
Vol 13 (1) ◽  
pp. 49-52
Author(s):  
Yunasri Usman ◽  
M. Nur Husin ◽  
Riantia Ratni
Keyword(s):  
Dry Matter ◽  
Animal Husbandry ◽  
Basal Diet ◽  
Animal Nutrition ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Coffee Beans ◽  
Completely Randomized Design

The supplementation peel coffee beans in the ration Aceh cattle on in vitro digestibilityABSTRACT. This research was carried out at the Laboratory of  Feed and Animal Nutrition,  Department of Animal Husbandry Faculty of Agriculture Syiah Kuala University for  73 days from December 26, 2011 to March 7, 2012. The purpose of this experiment was to find out the effects of the coffee peeling on in vitro digestibility of Aceh beef cattle. Completely Randomized Design (CRD) was applied in this experiment consisting of 4 treatments  namely levels of  coffee peeling on basal diet   (R0=0%, R1=10%, R2=20%, R3=30%). Each treatment consists of 5 replications. Parameters observed in this research were dry and organic matter digestibility. The results showed that dry matter digestibility was not significantly different (P0,05)amongst treatments  54,42%, 49,32%, 45,97%, 42,08%) for R0, R1, R2 and R3 respectively.  On the other hand, organic metter digestibility was significantly different (P0,05) in which R2 treatment was the highest and R0 treatment was the lowest (54,59%).

scholarly journals Evaluasi Kecernaan In Vitro Complete Feed Fermentasi Berbahan Dasar Ampas Sagu dengan Teknik Fermentasi Berbeda

2016 ◽  
Vol 1 (1) ◽  
pp. 808-815
Author(s):  
Sugrahadi Ahmad Aprianto ◽  
Yunasri Usman ◽  
Asril Asril
Keyword(s):  
Organic Matter ◽  
Dairy Cattle ◽  
Dry Matter ◽  
Animal Husbandry ◽  
In Vitro Digestibility ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Organic Matter Digestibility

Abstrak: Penelitian ini telah dilaksanakan di Laboratorium Ilmu Nutrisi dan Makanan Ternak Program Studi Peternakan Fakultas Pertanian Universitas Syiah Kuala dan dilanjutkan dengan pengiriman sampel penelitian ke Laboratorium Ternak Perah Institut Pertanian Bogor (IPB) untuk pengujian kecernaan secara in vitro. Tujuan dari penelitian ini adalah menguji kecernaan secara in vitro complete feed berbahan dasar ampas sagu dengan teknik fermentasi yang berbeda.Pembuatan complete feed disusun sesuai dengan kebutuhan ternak domba. Penelitian ini menggunakan Rancangan Acak lengkap (RAL) dengan 3 perlakuan dan masing-masing perlakuan terdiri atas 5 ulangan. Perlakuan 1 (kontrol) adalah complete feed tanpa penambahan Saus Burger Pakan (SBP) dan tanpa fermentasi, perlakuan 2 adalah ampas sagu difermentasi dengan SBP selama 14 hari kemudian dicampur menjadi complete feed dan difermentasi hingga 21 hari, sedangkan perlakuan 3 adalah ampas sagu + complete feed kemudian difermentasi dengan SBP selama 21 hari. Parameter yang diamati dalam penelitian ini adalah pH, Bahan Kering (BK), Bahan Organik (BO), Koefisien Cerna Bahan Kering (KCBK), dan Koefisien Cerna Bahan Organik (KCBO). Hasil pengamatan menyimpulkan bahwa, teknik fermentasi yang berbeda berpengaruh sangat nyata (P0,01) terhadap semua parameter yang diamati, yaitu pH, BK, BO, KCBK dan KCBO. Dari hasil penelitian dapat disimpulkan bahwa nilai kecernaan complete feed berbahan dasar ampas sagu dipengaruhi oleh perbedaan teknik fermentasi. Digestibility Evaluation In Vitro Complete Feed Fermentation Sago Residues Based with Different Fermentation Techniques Abstract: This research was conducted at the Laboratory of Nutrition and Feed Studies, Program study Animal Husbandry, Faculty of Agriculture, University of Syiah Kuala and continued with the delivery of sample to the Laboratory of Dairy Cattle Institute Pertanian Bogor (IPB) for testing of in vitro digestibility. The purpose of this study was to test in vitro digestibility of complete feed made from  the residues of sago with different fermentation techniques. Complete feed was arranged based on the needs of sheep. This study used a complete randomized design (CRD) with 3 treatments and each treatment consisted of 5 replicates. Treatment 1 (control) was a complete feed without the addition of Saus Burger Pakan (SBP) and without fermentation, treatment 2 was sago residu  fermented with SBP for 14 days and then mixed into a complete feed and fermented up to 21 days, whereas treatment 3 was the sago residue + complete feed then fermented with SBP for 21 days. The parameters observed in this study were pH, Dry Matter (BK), Organic Matter (BO), Dry Matter digestibility coefficients (KCBK), and Organic Matter Digestibility Coefficient (KCBO). The result influence showed that, different fermentation technique was significantly (P 0.01) all parameters, namely pH, BK, BO, KCBK and KCBO. It can be concluded that the digestibility of complete feed made from sago residue influenced by differences in fermentation techniques

scholarly journals PENGARUH PERLAKUAN FERMENTASI DAN AMONIASI KULIT SINGKONG TERHADAP NILAI KECERNAAN BAHAN KERING DAN BAHAN ORGANIK RANSUM PADA DOMBA JANTAN

2020 ◽  
Vol 4 (2) ◽  
pp. 119-125
Author(s):  
Agung Dwi Nugroho ◽  
Muhtarudin Muhtarudin ◽  
Erwanto Erwanto ◽  
Farida Fathul
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Animal Feed ◽  
Animal Husbandry ◽  
Experimental Unit ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Organic Matter Digestibility ◽  
Cassava Peels ◽  
Male Sheep

This research aimed to determine the effect of fermentation and ammoniation treatment of cassava peels on ration on dry matter digestibility (DMD) and organic matter digestibility (OMD) in male sheeps. This research was conducted in May--June 2019 in the cage unit and the Laboratory of Nutrition and Animal Feed, Department of Animal Husbandry, Faculty of Agriculture, University of Lampung. The experimental design applied was a completely randomized design (CRD) with 3 treatments and 3 replications. The experimental unit used were 9 sheeps. The treatments given were ration+15% cassava peels without treatment (R0), ration+15% fermented cassava peels (R1), and ration+15% ammoniated cassava peels (R2). The results showed that the use of cassava peel fermentation and ammoniation treatment had a significant effect (P<0.05) on dry matter digestibility (DMD) and organic matter digestibility in male sheep. The average of total digestibility of dry matter and organic matter of ration showed an increase of digestibility. The treatment of fermentation and ammoniation gave the best effect when compared to that without processing.   Keywords: Ammoniation, Fermentation, Cassava peels, Dry matter digestibility and organic matter digesbility, Sheep

scholarly journals In Vitro Study of Fermented Complete Feed by Using Sago Residues as Main Source Diet

2015 ◽  
Vol 17 (3) ◽  
pp. 129
Author(s):  
Samadi Samadi ◽  
Sitti Wajizah ◽  
Yunasri Usman
Keyword(s):  
Animal Feed ◽  
In Vitro Study ◽  
In Vitro Digestibility ◽  
Fermentation Products ◽  
Feed Formulation ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Coconut Meal ◽  
By Products

Recently, fermentation of low quality feed such as agro industry by products has been widely applied to produce enriched animal feed and improve animal productivity. The purpose of this study was to evaluate in vitro digestibility of fermented complete feed by using agro-residues from sago starch processing industries as main source diet.  Feed was formulated on the basis of 40% sago residue and mixed with other ingredients (rice brand, coconut meal, bread by product, soybean meal and soybean hulls) to fulfill the requirement of sheep with 16,10% of CP, 2,80 Mkal ME/kg and TDN 60,88% based on calculation. Mineral and Urea were added in the complete feed to reach mineral and CP requirements of sheep.  Complete feed formulation was fermented by using 3 commercial fermentation products (Saus Burger Pakan® (SBP), Probion® and EM4®) for 21 days. Total and types of microbes from commercial fermentation products which were utilized in this study were not determined. Parameters measured in this study were pH, NH3, IVDMD (in vitro dry matter digestibility), IVOMD (in vitro organic matter digestibility) and VFA total. The model used for the statistical analysis was completely randomized design (CRD) with 4 treatments (control and 3 different commercial fermentation products) and 4 replications.  The results of this study indicated that administration of different commercial fermentation products into fermented complete feed based on sago residues significantly influenced (P<0.05) on pH, NH3, VFA total, IVDMD and IVOMD. Generally, commercial fermentation products mainly SBP produced better feed quality by improving the values of pH, NH3, VFA total, IVDMD and IVOMD. However, types and total microorganisms were needed to be determined before experiment.

scholarly journals The use of coconut pulp as a feed substrate to methanogenesis inhibitor in in vitro rumen fluid fermentation

2017 ◽  
Vol 42 (3) ◽  
pp. 202
Author(s):  
E.H.B. Sondakh ◽  
J.A.D. Kalele ◽  
F.S. Ratulangi
Keyword(s):  
Microbial Activity ◽  
Rumen Fluid ◽  
Mean Values ◽  
Fatty Acids Profile ◽  
Feed Digestibility ◽  
Randomized Design ◽  
Methane Reduction ◽  
Range Test ◽  
Ruminant Feed

This experiment was conducted to determine the effect of the adding of coconut pulp in ruminant feed on methane reduction in vitro. Rumen fluid and substrate of forage and concentrate in ratio 60:40 were in this study. This experiment consisted of five treatments of coconut pulp as followed: R1: feed substrates without the coconut pulp; R2: substrate feed with coconut pulp 5%; R3: substrate feed with coconut pulp 10%; R4: Substrate feed with coconut pulp 15% and R5: substrate feed with coconut pulp 20%. Each treatment was conducted to four replications. Fermentation was done using the Hohenheim Gas Test (HGT) incubated at 39oC for 72 hours. At the end of fermentation was determined production of methane, parameters of fermentation, microbial activity, feed digestibility and fatty acids profile of the ruminal fluid. Data obtained were analyzed by using analysis of variance using completely randomized design. The differences of mean values were analyzed by Duncan multiple range test (DMRT). The results showed that methane production has decreased about 13.74%-17.39% when the feed was given coconut dregs 15-20%. Based on the results of the study was concluded that the supplementation of coconut pulp up to 15-20% of 100% dry matter can be used as a substrate of ruminant feed, because it can reduce methane and does not interfere the microbial activity in the fermentation of rumen fluid through in vitro. The supplementation of coconut pulp can be a referencein designing the methanogenic inhibitor feed.

scholarly journals PSIX-12 Screening of peptides for their impact of protease, protease dose and peptide dose on in vitro rumen dry matter digestibility

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 398-399 ◽  
Author(s):  
Wenzhu Yang ◽  
YiZhao Shen ◽  
Ranithri Abeynayakea ◽  
Tao Ran ◽  
Lingyun Chen
Keyword(s):  
Dry Matter ◽  
Barley Grain ◽  
Batch Cultures ◽  
Dry Matter Digestibility ◽  
Feed Digestibility ◽  
Randomized Design ◽  
Optimum Ph ◽  
Spent Grain ◽  
Barley Silage

Abstract The objective of this study was to screening peptides for the effects on in vitro rumen dry matter (DM) digestibility (DMD) of feeds in batch culture. Three brewers spent grain (BSG) samples from different batches were obtained from a local brewery. Peptides were developed with BSG protein varying protease (alcalase, everlase, flavourzyme) and protease dosages (protease:BSG protein; 1:100, 5:100, 10:100, 15:100) under optimum pH and temperature conditions. In total, 36 peptides were produced and screened. A series of batch cultures were conducted and substrates were barley silage and barley grain. The experiment was a complete randomized design with a factorial arrangement; 3 proteases × 4 protease doses + 4 doses of each peptide (0, 0.5, 1.0, 1.5% of substrate DM) with 3 replications of each treatment combination. Rumen inoculum was from three rumen fistulated beef heifers fed a diet consisting of 10% silage and 90% concentrate (DM basis). The incubation was conducted at 39°C for 24 h. Interactions among protease, protease dose and peptide dose were not significant. For barley grain, DMD differed (P &lt; 0.01) among proteases (85.2, 72.3, 65.2%, respectively, for alcalase, flavourzise, everlase). Increasing peptide doses from 0, 0.5, 1.0 to 1.5% linearly (P &lt; 0.01) decreased the DMD from 88 to 78% (alcalase), 69 to 59% (everlase) and 78 to 67% (flavourzyme); whereas protease dose did not affect the DMD. With barley silage, the DMD was higher (P &lt; 0.05) with flavourzyme (65.3%) than alcalase (59.2%) or everlase (61.2%); in contrast, the DMD was affected neither by protease dose nor by the peptide dose, except that increasing supplementation of peptide derived from everlase linearly (P &lt; 0.05) decreased the DMD (65.1 to 58.9%). These results indicated that the peptides derived from BSG decreased in vitro rumen feed digestibility, which varied with proteases, peptide dose or feeds.

scholarly journals Thermostable Alkaline Phytase from Alcaligenes sp. in Improving Bioavailability of Phosphorus in Animal Feed: In Vitro Analysis

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ponnuswamy Vijayaraghavan ◽  
R. Raja Primiya ◽  
Samuel Gnana Prakash Vincent
Keyword(s):  
Inorganic Phosphate ◽  
Animal Feed ◽  
Alkaline Phytase ◽  
Optimum Ph ◽  
Green Pea ◽  
Ph Range ◽  
Vitro Analysis ◽  
Optimum Ph Range ◽  
In Vitro Analysis

A bacterial isolate, Alcaligenes sp. secreting phytase (EC 3.1.3.8), was isolated and characterized. The optimum conditions for the production of phytase included a fermentation period of 96 h, pH 8.0, and the addition of 1% (w/v) maltose and 1% (w/v) beef extract to the culture medium. This enzyme was purified to homogeneity and had an apparent molecular mass of 41 kDa. The optimum pH range and temperature for the activity of phytase were found to be 7.0-8.0 and 60°C, respectively. This enzyme was strongly inhibited by 0.005 M of Mn2+, Mg2+, and Zn2+. In vitro studies revealed that the phytase from Alcaligenes sp. released inorganic phosphate from plant phytates. Phytase released 1930 ± 28, 1740 ± 13, 1050 ± 31, 845 ± 7, 1935 ± 32, and 1655 ± 21 mg inorganic phosphate/kg plant phytates, namely, chick pea, corn, green pea, groundnut, pearl pea, and chick feed, respectively.

The native structure of the eukaryotic ribosome

1983 ◽  
Vol 41 ◽  
pp. 432-433
Author(s):  
R.A. Milligan ◽  
P.N.T. Unwin
Keyword(s):  
Ribosomal Proteins ◽  
Crystal Form ◽  
Native Structure ◽  
X Ray Diffraction ◽  
Eukaryotic Ribosome ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Resolution Structure ◽  
Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

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