scholarly journals EFEKTIFITAS BAKTERI ENDOFIT DAN PENAMBAHAN INDOLE ACETIC ACID (IAA) DALAM MENINGKATKAN PERTUMBUHAN TANAMAN PADI Oryza sativa L.

2017 ◽  
Vol 4 (3) ◽  
pp. 177
Author(s):  
Aria Rizki Ramadhan ◽  
Oedjijono Oedjijono ◽  
Ratih Dewi Hastuti
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Rice Plant ◽  
Plant Root ◽  
Rice Variety ◽  
Combined Treatment ◽  
Soil Biology ◽  
Randomized Design ◽  
Two Factors ◽  
Different Doses

Rice plant needs essential and non-essential nutrients to grow. However, the use of inorganic fertilizers may affect the soil microbe community which expected to increase the availability of nutrients for both plants and microbes, a condition that could control several types of diseases. Many efforts were made to find alternative fertilizer which environmentally friendly, for instance by exploiting microorganisms such as endophytic bacteria associated with plant tissue or seed plant cell. This research aimed to determine the effect of endophytic bacteria, the concentration of IAA, and the combination of endophytic bacteria with a concentration of IAA, on the growth of rice plant. This research was conducted at the Laboratory of Soil Biology, The Soil Research Institute located in Cimanggu Bogor from December 2015 to February 2016. This research was a Completely Randomized Design (CRD) with two factors, i.e., the inoculant of endophytic bacteria (2.2 KT, KR 6, and I CM), and the concentration of IAA (0, 0.01, 0.1, 0.5, and 1 ppm). Three replicates were applied for each treatment of the inoculant combined with different doses of IAA. The variety of rice plants tested were Inpari 13 and IR 64. The potential test has been applied to determine the factor with the highest yield of the rice plant growth. The parameters observed were the height of the rice plant, the length of the rice plant roots, and the total weight of the plant including root. The results showed the inocula of endophytic bacteria alone had no effect to increase growth for both IR 64 and Inpari 13 rice variety. The IAA concentration of 0.1 ppm was able to increase the height of rice plant and the length of rice plant root for both IR 64 rice plant and Inpari 13. The combined treatment of KR 6 bacterial inoculum with 1 ppm of IAA concentration was effectively promoted rice plant growth for both IR 64 and Inpari 13.

scholarly journals Pola Interaksi Bakteri Endofitik +GFP (Green Fluorescent Protein) dalam Jaringan Tanaman Padi (oryza sativa l.)

2019 ◽  
Vol 2 (3) ◽  
pp. 255
Author(s):  
Sufianto Sufianto
Keyword(s):  
Plant Tissue ◽  
Endophytic Bacteria ◽  
Rice Plant ◽  
Fluorescent Protein ◽  
Oryza Sativa L ◽  
Bacterial Interactions ◽  
Randomized Design ◽  
Green Fluorescent ◽  
Two Stages

This study was intended to examine the pattern of interaction and mutant distribution of endophyticbacteria as a result of the transformation of the gfp gene in rice plant tissue in vitro. This study used 5isolates of rice endophytic gfp mutants namely IM-1, IM-3, IM-8, IM-24, IM-25. The study wasconducted in two stages, stage I using a simple Complete Complete Design (RAL) and stage II usingfactorial Complete Randomized Design (RAL) with a factor of 1: the type of plant tissue and factor 2:the type of mutant isolates.The results of the study of patterns of bacterial interactions in rice tissueculture showed that the percentage of infections of 5 gfp mutant endophytic bacteria isolates rangedfrom 4% -17%. The highest percentage was in mutant isolates IM-3 + gfp (17%) and the lowest was inmutants IM-8 + gfp (4%). The interaction pattern was not significantly different from the parametersof seed germination time. On the other hand, in observing the distribution of bacteria that infect ricetissue, they showed different pattern. Mutant IM-1 + gfp infection is higher in stem tissue, whereasIM-24 + gfp mutant infection is higher in root tissue and IM-25 + gfp mutant infection is higher inleaf tissue. Mutant infection of IM-25 + gfp in leaves had the highest number of colonies reaching1.64 x 109 cfu/ml.

scholarly journals Response of Shallots of Batu Ijo Variety on Doses of N And K Fertilizers

Akta Agrosia ◽  
2021 ◽  
Vol 24 (1) ◽  
pp. 19-24
Author(s):  
Widodo Widodo ◽  
Marlin Marlin ◽  
Nancy B. Sitio
Keyword(s):  
Plant Growth ◽  
N Fertilizer ◽  
Tuber Weight ◽  
Plant Weight ◽  
Randomized Design ◽  
K Fertilizer ◽  
Two Factors ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Higher Doses

Shallots (Allium cepa var. Aggregatum L) have many varieties that can be grown in the highlands and lowlands and one of them is the Batu Ijo variety. Nutrients N and K play an important role for plant needs. This research was conducted from March to June 2019 in Medan Baru, Bengkulu City. This study used a completely randomized design with two factors. The first factor was the dose of N fertilizer and the second factor was the application of K fertilizer. The application of N fertilizer had a significant effect on the number of shallots at week 2. The Urea dose given decreases the number of spring onions. For the best treatment at plant height is the treatment of Urea 300 kg/ha and KCl 50 kg/ha. Plant growth from week 2 to 5 increased significantly. The application of K fertilizer has an effect on the number of shallots at week 2 and the number of tubers. The higher the plant age, the more the number of leaves produced, but at higher doses, the number of leaves and the number of tubers decreased. The best treatment for the number of leaves produced was Urea 300 kg/ha and without giving KCl. For the best treatment of fresh plant weight, namely Urea 300 kg/ha and KCl 150 kg/ha, the best treatment fresh tuber weight was Urea 300 kg/ha and KCl 150 kg/ha, for tuber diameter the best treatment was 200 kg/ha of urea and KCl 100 kg/ha and for the number of tubers of Urea 0 kg/ha and KCl 150 kg/ha.Keywords: shallots, N fertilizer, K fertilizer

scholarly journals Effectiveness of Reducing P Fertilizer and Adding Fish Pond Mud Waste on Growth and Yield of Soybean in Peatland

2018 ◽  
Vol 31 ◽  
pp. 05005
Author(s):  
Erina Riak Asie ◽  
Nyahu Rumbang ◽  
Sih Winarti ◽  
Soaloon Sinaga
Keyword(s):  
Plant Growth ◽  
Plant Tissue ◽  
Growth And Yield ◽  
Fish Pond ◽  
Treatment Combination ◽  
P Concentration ◽  
Randomized Design ◽  
Two Factors ◽  
P Fertilizer ◽  
Sludge Waste

The objective of the study was to assess the effectiveness of P fertilizer reduction and the addition of fish pond sludge waste on the growth and yield of soybean crop in peatland. Research used Complete Randomized Design factorial with two factors. The first factor was the reduction of P fertilizer from the dose of 150 kg.ha-1 consisting of 4 levels, namely P0: 100% (2.944 g/polybag), P1: 75% (2.208 g/polybag), P2: 50% (1.472 g/polybag), and P3: 25% (0.736 g/polybag). The second factor was the addition of fish pond mud waste (L) from the dose of 15 ton.ha-1 consisting of 4 levels, namely L0: 25% (73.595 g/polybag), L1: 50% (147.19 g/polybag), L2: 75% (220.78 g/polybag), and L3: 100% (294.38 g/polybag). Each treatment combination was replicated 3 times to obtain 48 experimental units. The results showed that (1) fish pond mud waste was effective to reduce the use of P fertilizer, (2) the reduction of P fertilizer up to 50% from recommendation dosage by addition of fish pond sludge waste at 75% dose of 15 ton/ha was the best combination due to providing the best plant growth and the highest P concentration of plant tissue. The highest number of pods and weight of seed obtained in the combination were 60.33 pods/plant and 7.30 g/plant, respectively.

scholarly journals RESPONSE to Trichoderma spp. ON SEED VIGOR INDEX AND DRY WEIGHT OF RICE VARIETY SIRANDAH BATUAMPA VARIETY

2021 ◽  
Vol 8 (1) ◽  
pp. 1-6
Author(s):  
Rahmi Zahri Zani ◽  
Azwir Anhar
Keyword(s):  
Plant Growth ◽  
Rice Variety ◽  
Dry Weight ◽  
Seed Priming ◽  
Mean Value ◽  
Seed Vigor ◽  
Trichoderma Spp ◽  
Randomized Design ◽  
Vigor Index ◽  
Plant Growth Promoting

Rice is a staple food crop for some Indonesians. To increase rice production, various efforts have been made, one of which is seed priming using biofertilizers. Biofertilizer or biological fertilizer uses microorganisms that are used as plant growth boosters. The microorganisms used to act as PGPF (Plant Growth Promoting Fungi) are Trichoderma spp. Trichoderma spp. widely used because it can trigger plant growth. This study aims to determine the response of Trichoderma spp. to the seed vigor index and dry weight of rice sprouts of sirandah batuampa variety. The research method is experimental research using a completely randomized design (CRD) with 6 treatments and 3 replications. Parameters observed were seed vigor index and sprouts dry weight. The results showed that Trichoderma spp. had no significant effect on seed vigor index and sprouts dry weight. Judging from the mean value, the seed vigor index of SBT, SRBA, and KRT had better values ​​than the control, as well as the dry weight of sprouts with the provision of SBT, SRBA, and SB isolates. This shows Trichoderma spp. This gave a positive response to the Sirandah Batuampa variety.

scholarly journals PENGARUH Trichoderma sp. SEBAGAI AGEN PENINGKATAN KETAHANAN TANAMAN PADI TERHADAP PENYAKIT HAWAR DAUN

2019 ◽  
Vol 7 (3) ◽  
pp. 423
Author(s):  
Geraldo Sandy ◽  
Suskandini Ratih ◽  
Radix Suharjo ◽  
Hasriadi Mat Akin
Keyword(s):  
Rice Plant ◽  
Plant Disease ◽  
Leaf Blight ◽  
Bacterial Leaf Blight ◽  
Randomized Design ◽  
Trichoderma Sp ◽  
Two Factors

Rice is one commodity that have an important value for Indonesian people food. But the production of rice plant decreased by attacking of insect and bacterial. The bacterialleaf blight caused by Xanthomonas oryzaepv.oryzae. To avoid the bacterial leaf blight could used Trichoderma sp. suspension to induced resistant in rice plant. This research was conducted at the Laboratory of Plant Disease and Greenhouse of Lampung University. This study was conducted from June 2016 to August 2016.The experiment was conducted in a complete randomized design arranged with two factors, namely varieties of rice and kind isolate of Trichoderma sp.. The results of this study showed that the application of Trichoderma sp. reducing the severity of bacterial leaf blight diseaseand the kind isolate of Trichoderma sp. can increase the length rootand height of rice plant.

scholarly journals Isolation of Phosphate Solubilizing Endophytic Fungi From Rice Plant Root

BioScience ◽  
2018 ◽  
Vol 2 (1) ◽  
pp. 93
Author(s):  
Dezi Handayani ◽  
Mades Fifendy ◽  
Verawati Yesni
Keyword(s):  
Plant Growth ◽  
Endophytic Fungi ◽  
Rice Plant ◽  
Phosphate Solubilization ◽  
Plant Root ◽  
Phosphorus Uptake ◽  
Morphological Characteristics ◽  
Microscopic Appearance ◽  
Root Endophytic Fungi ◽  
Solubilization Ability

Root endophytic fungi plays different roles for plant, such as plant growth promoting properties, agents to control phytopathogens, and increase phosphorus uptake. Since phosphorus are essential for plant growth and its occurance are limited, so it is necessary to explore these fungus to replace the used of synthetic fertilizer. The objective of this study were to obtain root endophytic fungi from rice plant and to determine its phosphate solubilization ability. The root organ of rice plant was subjected for isolation. Pikovskaya medium was use to determine the fungal phosphorus solubilization ability. Fungal morphological characteristics was carried out by macroscopic and microscopic appearance assessment using microscope. Seven endophytic fungi were successfully isolated from rice plant root sample. Four isolate were micelial steril with no conidia, two isolate refers to Aspergillus and 1 isolate have 2-4 conidia at the tip of conidiophores. Amongs 7 endophytics fungi, only one isolate (P2B3) had the ability to solubilize phosphate with the phosphate solubilization index value 20.45 %.

scholarly journals Test Of The Effectiveness Of Root Alelopathic Extract (Cyperus rotundus L.) and Bandotan (Ageratum conyzoides L.) On The Growth Of Tomato Plants (Solanum lycopersicum L.)

Biocelebes ◽  
2019 ◽  
Vol 13 (3) ◽  
pp. 262-270
Author(s):  
Dewi Arini ◽  
Wahyu Harso ◽  
Asri Pirade Paserang
Keyword(s):  
Plant Growth ◽  
Tomato Plant ◽  
Cyperus Rotundus ◽  
Root Extract ◽  
Ageratum Conyzoides ◽  
Tomato Plants ◽  
Solanum Lycopersicum L ◽  
Randomized Design ◽  
Tomato Field ◽  
Two Factors

Cyperus rotundus L. and Ageratum conyzoides L. are commonly found in tomato field as weeds species. Weed compete with the crop for nutrient, water and light. In addition, weed released allelopathy that inhibited the growth of crop. The aim of this study was to compare the ability of allelopathy from C. rotundus L. and Ageratum conyzoides L. root extract to inhibit tomato plant growth. The study was conducted in Completey Randomized Design with two factors. The first factor was root extract from either C. rotundus L. and A. conyzoides L. as allelopathy. The second factor was concentration of root extract from both weeds (100, 300 and 500 g/ml). Each treatment combination was repeated four times. The results showed that A. conyzoides L. root extract had higher inhibition to growth of tomato plant than C. rotundus L. root extract. Increasing root extract concentration from both weeds increased inhibition of tomato plant growth.

scholarly journals The Endophytic Microbiome as a Hotspot of Synergistic Interactions, with Prospects of Plant Growth Promotion

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 101
Author(s):  
Udaya Kumar Vandana ◽  
Jina Rajkumari ◽  
L. Paikhomba Singha ◽  
Lakkakula Satish ◽  
Hemasundar Alavilli ◽  
...  
Keyword(s):  
Plant Growth ◽  
Microbial Communities ◽  
Plant Growth Promotion ◽  
Endophytic Bacteria ◽  
Host Plants ◽  
Plant Pathogens ◽  
Growth Promotion ◽  
Plant Root ◽  
Endophytic Microorganisms ◽  
Microbe Interactions

The plant root is the primary site of interaction between plants and associated microorganisms and constitutes the main components of plant microbiomes that impact crop production. The endophytic bacteria in the root zone have an important role in plant growth promotion. Diverse microbial communities inhabit plant root tissues, and they directly or indirectly promote plant growth by inhibiting the growth of plant pathogens, producing various secondary metabolites. Mechanisms of plant growth promotion and response of root endophytic microorganisms for their survival and colonization in the host plants are the result of complex plant-microbe interactions. Endophytic microorganisms also assist the host to sustain different biotic and abiotic stresses. Better insights are emerging for the endophyte, such as host plant interactions due to advancements in ‘omic’ technologies, which facilitate the exploration of genes that are responsible for plant tissue colonization. Consequently, this is informative to envisage putative functions and metabolic processes crucial for endophytic adaptations. Detection of cell signaling molecules between host plants and identification of compounds synthesized by root endophytes are effective means for their utilization in the agriculture sector as biofertilizers. In addition, it is interesting that the endophytic microorganism colonization impacts the relative abundance of indigenous microbial communities and suppresses the deleterious microorganisms in plant tissues. Natural products released by endophytes act as biocontrol agents and inhibit pathogen growth. The symbiosis of endophytic bacteria and arbuscular mycorrhizal fungi (AMF) affects plant symbiotic signaling pathways and root colonization patterns and phytohormone synthesis. In this review, the potential of the root endophytic community, colonization, and role in the improvement of plant growth has been explained in the light of intricate plant-microbe interactions.

scholarly journals Potensi dan Karakterisasi Fisiologis Bakteri Endofit Asal Tanaman Kakao Sehat sebagai Pemacu Pertumbuhan Benih Kakao

2020 ◽  
Vol 25 (3) ◽  
pp. 388-395
Author(s):  
Andi Khaeruni ◽  
Tanza Nirmala ◽  
Waode Siti Anima Hisein ◽  
Gusnawaty Gusnawaty ◽  
Teguh Wijayanto ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Plant Protection ◽  
Seed Viability ◽  
Dry Weight ◽  
Control Treatment ◽  
Laboratory Unit ◽  
Randomized Design ◽  
Plant Growth Promoting ◽  
Growth Promoting

This study aimed to obtain endophytic bacterial isolates originated from healthy cacao plant, potential for plant-growth promoting of cacao seedlings. This study was carried out in the Phytopathology Laboratory Unit of the Plant Protection Department, Faculty of Agriculture, Halu Oleo University. This study was conducted using a completely randomized design (CRD) using ten isolates of endophytic bacteria from healthy cocoa plants as treatments, plus a control treatment. The experiment was conducted in a screenhouse using seed treatment and planted in seedling boxes, containing sterile rice-husk charcoal as a growing medium. The result showed that  three endophytic bacteria tested (isolates 2RPR1, 2RWB2, and 5BRB3) were potential as plant growth-promoting of cocoa seedlings, because the isolates were able to increase seed germination up to 96.67%, relative growth rate up to 90-96.67%, increased plant height up to 47.85-67.17%, root dry weight up to 35.08-52.63%, and canopy dry weight up to 97.71-108.46%. The superiority of the three isolates were related to their abilities to fix nitrogen, dissolve phosphate, as well as to produce indole acetic acid.   Keywords: cacao, endophytic bacteria, plant growth-promoting agent, seed viability

scholarly journals Callus Induction, Regeneration and Establishment of Rice Plant from Mature Embryo

2021 ◽  
pp. 10-18
Author(s):  
Nazmul Alam Khan ◽  
Md. Imtiaz Uddin ◽  
Md. Shohel Rana ◽  
Nusrat Jahan ◽  
Mirana Akhter Sumi ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Rice Plant ◽  
In Vitro Regeneration ◽  
Rice Variety ◽  
Development Program ◽  
Regeneration Ability ◽  
Ms Medium

Plant growth regulators were used to test callus induction and in vitro regeneration in six rice genotypes (RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97). Four different concentrations (1, 2, 3 and 4 mg/L) of 2,4-D for callus induction and three different concentrations (1,2 and 3 mg/L) of NAA with three doses (5,10 and 15 µ/L) of kinetin for callus regeneration were used to test the effect of plant growth regulators. This study found a high callus induction on MS medium enriched with 2 mg/L 2, 4-D. In cases of RM-AC-2, BRRI dhan89, BRRI dhan88, Nipponbare, Koshihikari and Zenshan97, callus induction frequencies were 92.7%, 87.8%, 84.6%, 82.9%, 86.2% and 62.9%, respectively. In the regeneration, it was found that an MS medium enriched with 2 g/L Kinetin and 10 µm/L NAA has the ability to induce increased regeneration of different rice varieties (RM-AC-2 (72.4%), BRRI dhan89 (66.9%), BRRI dhan88 (62.5%), Nipponbare (63.3%), Koshihikari (48%) and Zenshan97 (39.6%). From the regenerated plants, one plant of the RM-AC-2 genotype availed to complete its life cycle and generated 32 effective tillers and yielded 89g. This rice plant is very promising for high yielding rice variety development program in Bangladesh. The improved callus development and regeneration ability of this genotype might be helpful for future rice variety development and genetic transformation program.

Sign in / Sign up

Export Citation Format

Share Document