scholarly journals Microbial approach of epitope tagged MFE-23 single fragment antibodies production

2020 ◽  
Vol 11 (3) ◽  
pp. 224-231
Author(s):  
Razmaeda Sarastry
Keyword(s):  
1984 ◽  
Vol 16 (3-4) ◽  
pp. 433-440
Author(s):  
O C A Iriberri

Coastal zone management requires an understanding of the complex milieu of interactions and activities taking place in an environmental system. Man is beginning to recognize that the old method of dealing with individual issues and problems as single fragment of a whole ecosystem is not enough. This paper tries to deal with the integrated manner in carrying out effectively the management of the coastal zone in Puerto Galera, Oriental Mindoro by the Man and the Biosphere Interagency Committee on Ecological Studies. To attain the objective of the project, the different agencies monitor, identify, observe, investigate various natural and physical parameters contributing to the ecological balance and study the rational use of the resources along the coastal zone. Result of the study showed that although such factors as land use practices of shifting cultivation (kaingin), human attitude towards forest and its resources, and continuous increase in population and migration of people were observed, such pressure on lands has not greatly affected the Puerto Galera coastal zone resources.


This new species of Bennettites , the first petrified remains of the group to be found in the Gault of this country, is based on the structure of an ovulate cone. While it it true that only a portion of one cone is available, yet even this fragment includes hundreds of exquisitely preserved seeds which throw much new light on the structure of that most important group, the Bennettitales. Since the historic description so many years ago of the one and only specimen of the type of the group, B. Gibsonianus (see Carruthers, 1870), no other well petrified cone has been found in this country, and knowledge of the details of the fructifications of the group has been advanced chiefly by studies of the French B. Morierei , and a number of American forms. Beautiful as some of these are, the present specimen surpasses anything yet known in the Bennettitales for perfection of detailed preservation. Many points in the new species are novel, and on variations of less magnitude new fossil genera have been founded; but as in essentials the fruit conforms to the broad type “ Bennettites ,” I prefer to use that widely known generic name for the new species. The material from which the following description is compiled consists of a single fragment of a large ovulate cone. This was cut into as many sections as possible, but yielded only two longitudinal and seven transverse sections. Magnified by two diameters, five of this series of transverse sections are shown on Plate 19.


2016 ◽  
Vol 22 (2) ◽  
pp. 81-88
Author(s):  
Andrzej Jakubowski ◽  
Renata Crasto

Abstract The details of making technology of porous permeable material with use of wire are allowed to carry out the analytical research of structure and structural characteristics of wire winding body. Its permit for prognostication the final properties of material, that is produced by the following deformation treatment (diameter reduction). Due to the regular organized arrangement of wire, the coil of winding body is considered as a multispan continuous beam, but a contact of coils - as interaction of two cylinders. Possibility of exactly calculation of the contacts between coils is allowed to go over the single fragment displacements into deformation of whole winding body. During research of deformation processes in regards of winding body geometry and used wire mechanical properties, the structural characteristics of porous permeable wire material are expected. The optimal number of winding layers, eliminating the distortion of organized final structure, is established. The material pressure-compactness relation is obtained in order to control the technological conditions of winding and drafting for guarantee the product required properties.


2011 ◽  
pp. 3747-3753
Author(s):  
Monica Zuccarini

Information technology is changing the role of government, its functions, and its power. The long process of transformation of public sector organizations and the applications of computer technologies have started the talk about electronic government. In the last ten years, the debate concerning e-government has been the subject of various studies, promoting analyses, research approaches, and empirical surveys. Even so, there is still much confusion about the meaning of this term and the word e-government is often used in reference to e-voting, e-democracy, or e-administration. Therefore here we intend to provide the reader with some guidelines, trying to explain a concept that, although little more than ten years old, finds its roots in the ancient idea of the information society. We can start by saying that e-government represents just a single fragment of the broad discourse about the impact of information technologies on society. It is not an administrative experiment but a permanent part of the governmental process. According to Jane Fountain’s (2004) idea, we can define e-government as “the production and delivery of information and services inside government and between government and the public using a range of information and communication technologies” (para. 2). In this prospective, the Internet can be considered as a variable, which has been grafted onto the wider ground of factors leading to the transformation of government roles. Moving from this point, e-government will be analyzed as an intersection of two main trends. The first of these is the overall transformation of public sector organization. In this respect, the Internet variable has to be considered in the entire process of the government reinvention that made scholars talk about a major new revolution after the industrial one. The second trend is the information society development, a process that started since the 50s catching very soon the interest of governments.


2009 ◽  
Vol 45 (No. 1) ◽  
pp. 11-17 ◽  
Author(s):  
K. Jomová ◽  
M. Benková ◽  
J. Kraic

A set of 49 chickpea accessions maintained in the collection of the Genebank of the Slovak Republic was analyzed using microsatellite markers. The level of genetic diversity and the effectivity of new germplasm acquisition for the collection enrichment were evaluated. Five primer pairs used generated 50 different amplified alleles. Four of them, TA2, TA5, TR1, and TR7, containing a long TAA-tandem repeat, were polymorphic with 11–13 alleles per locus. A single fragment was obtained from all the accessions with the primer pair CATPER flanking a small microsatellite repetition in the mRNA sequence of chickpea cationic peroxidase. The genetic diversity was expressed as a diversity index (DI) and polymorphic information content (PIC) with values in the range from 0.885 to 0.904 and from 0.972 to 0.991, respectively. Recently acquired new germplasm contributed 13 new alleles to the entire collection, i.e. almost 70% of microsatellite diversity originated from the recently acquired accessions. Remarkable is also, that one accession carries a unique allel, not present in the remaining collection. Microsatellite analysis revealed the importance of collecting missions for the enrichment of collections of genetic resources.


2010 ◽  
Vol 46 (No. 4) ◽  
pp. 171-180 ◽  
Author(s):  
O. Douda ◽  
M. Zouhar ◽  
E. Nováková ◽  
J. Mazáková ◽  
P. Ryšánek

Potato cyst nematodes (Globodera rostochiensis, Globodera pallida) remain a key pest in the main potato growing regions of the Czech Republic. Due to difficult direct management and presence of diverse pathotypes attacking different potato cultivars the rapid and reliable diagnostics is of crucial importance. Currently, efforts are aimed at a description of different pathotypes based on DNA analysis. The main objective of this study was to evaluate the homogeneity of sequences of D2/D3 segments of the 28S rDNA gene obtained from 3 populations of G. rostochiensis and 5 populations of G. pallida and estimate their value for diagnostic purposes. PCR amplification yielded a single fragment of the length of 700 bp approximately in all populations. The alignment score of the vast majority of all pair comparisons of G. rostochiensis and G. pallida populations varied from 98 to 99. In total 14 point deletions and 3 substitutions were observed. The variability of D2/D3 segments of potato cyst nematodes is rather low and this DNA region can be used for diagnostics on a species level because more differences were found after comparing with G. tabacum and G. millefolii sequences obtained from Gene Bank; however the applicability of D2/D3 sequences to routine diagnostics of potato cyst nematodes could be complicated by its similarity to corresponding sequences of the nematode G. artemisiae.


1945 ◽  
Vol 2 ◽  
pp. 25-28
Author(s):  
James A. Ford ◽  
George I. Quimby

It appears that in the original excavations of the Little Woods site Czajkowski found skeletons in the middens at different points. The following is taken from his article in the Louisiana Conservation Review:Most of the burials on the shell ridge were solitary and no funeral furniture or artifacts were found with them, with two exceptions: (1) On March 20th, two skeletons were found in a semi-flexed, east-west position. The following day, five more skeletons were uncovered, making a total of seven, all lying in the same position, parallel to each other about four feet apart, at a depth of about five feet …(2) On June 1st at a depth of six feet, a single skull impaled on a tree stump was found. Underneath the skull was a single fragment of decorated pottery.Burial Ground: Seven skeletons found on March 20th, 950 feet northwest from the location. Adults, in fairly good condition; all skulls crushed except one…. Six in semi-flexed position on their backs … (the illustration to which Czajkowski here refers shows a fully flexed skeleton). All lying parallel in an east-west position with their heads west. The seventh at full length on its face. They were lying at a depth of about four feet from the surface and overlain with a shell bed from two to twelve inches in thickness. The distance between the skeletons was from three to four feet (Czajkowski, 1934, pp. 14-15).


Author(s):  
L. Fletcher

Single fragment of iron, having an estimated weight of eleven pounds, was found about the year 1880 on or near the top of Alleghany Mountain, 3 miles north of White Sulphur Springs, Greenbrier County, not far from the eastern border of West Virginia, U.S.A. ; this corresponds to longitude 80° 20' W. of Greenwich, latitude 37° 51' N. The finder and his official agent, thinking it a piece of rich iron ore, searched unsuccessfully for a vein : the specimen itself was taken to a country smith's shop, heated and cut with a cold chisel; the pieces were distributed as specimens of iron ore. Some time afterwards, two of them, weighing respectively 63 oz. and 31 oz., were given by the agent to Mr. Matthew A. Miller, Civil Engineer, of Richmond, Virginia ; convinced of their meteoric origin, he immediately tried to recover the pieces already distributed, but after travelling several hundred miles was forced to the conclusion that they were irrecoverably lost. From Mr. Miller the two pieces were acquired for the British Museum.


Author(s):  
R. Litvinchuk ◽  
A. Levchenko

The article considers a number of main factors that determine the probability of damage to the elements of the engine and combat compartment of lightly armored vehicles under the influence of fragmentation munitions. Also, the technique of calculation of probability of defeat of an element by one fragment is given. As a result, the idea of a graph- analytical method for determining the probability of hitting an element by one fragment is presented, which is that the set of possible flight of the fragment to the element is divided into subsets by the number of walls of the engine and combat compartment of lightly armored vehicles. Then the required value of the probability of damage to the element by one fragment is determined.One of the main striking factors is the rapid flow of fragments that hit lightly armored vehicles, thus reducing the combat readiness of the unit. At present, the question of calculating the probability of damage to the elements of lightly armored vehicles of general military units is insufficiently studied.During the calculations of the graph-analytical method for determining the probability of damage to the elements of the engine and combat compartment of lightly armored vehicles, attention was focused on determining the required value of the probability of damage to the element by one fragment, which has not been proposed so far.The idea of a graph-analytical method for determining the probability of damage to an element by a single fragment is as follows. The whole set of directions of possible flight of the fragment to the element is divided into subsets by the number of walls of the engine and combat compartment of lightly armored vehicles. Within each subset, all directions are divided into parts, within each of which the value hijs is constant.The values of Sijs and hijs are determined for each part. The value of Sijs determines the probability of impact of the fragment from these directions, and the value of hijs - the probability of breaking the protective barrier.Then the required value of the probability of damage to the element by one fragment is determined. Thus, the use of a graphical-analytical method to determine the probability of damage to the elements of lightly armored vehicles of all-military units will increase the possibility of evacuation and restoration of weapons and military equipment after destruction by shrapnel munitions.And reducing the time for evacuation, speeding up the process of restoring damaged equipment in combat and increasing the intensity of the recovery system from recovery to use will increase the likelihood of using recovered equipment and, consequently, increase combat readiness.


2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Gang Liu ◽  
Xiaoguang Guo ◽  
Qian Zhao ◽  
Bo Qin ◽  
Junjie Lu ◽  
...  

Abstract Background Split fractures of the humeral greater tuberosity (HGT) are common injuries. Although there are numerous surgical treatments for these fractures, no classification system combining clinical and biomechanical characteristics has been presented to guide the choice of fixation method. Methods We created a standardised fracture of the HGT in 24 formalin-fixed cadavers. Six were left as single-fragment fractures (Group A), six were further prepared to create single-fragment with medium size full-thickness rotator cuff tear (FT-RCT) fractures (Group B), six were cut to create multi-fragment fractures (Group C), and six were cut to create multi-fragment with FT-RCT fractures (Group D). Each specimen was fixed with a shortened proximal humeral internal locking system (PHILOS) plate. The fixed fractures were subjected to load and load-to-failure tests and the differences between groups analysed. Results The mean load-to-failure values were significantly different between groups (Group A, 446.83 ± 38.98 N; Group B, 384.17 ± 36.15 N; Group C, 317.17 ± 23.32 N and Group D, 266.83 ± 37.65 N, P < 0.05). The load-to-failure values for fractures with a greater tuberosity displacement of 10 mm were significantly different between each group (Group A, 194.00 ± 29.23 N; Group B, 157.00 ± 29.97 N; Group C, 109.00 ± 17.64 N and Group D, 79.67.83 ± 15.50 N; P < 0.05). These findings indicate that fractures with a displacement of 10 mm have different characteristics and should be considered separately from other HGT fractures when deciding surgical treatment. Conclusions Biomechanical classification of split fractures of the HGT is a reliable method of categorising these fractures in order to decide surgical treatment. Our findings and proposed system will be a useful to guide the choice of surgical technique for the treatment of fractures of the HGT.


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