scholarly journals Modulation of ATP8B1 Gene expression in Colorectal Cancer Cells Suggest its Role as a Tumor Suppressor

2020 ◽  
Author(s):  
Saleh Althenayyan ◽  
Amal AlGhamdi ◽  
Mohammed H AlMuhanna ◽  
Esraa Hawsa ◽  
Dalal Aldeghaither ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acid ◽  
Tumor Suppressor ◽  
Cell Line ◽  
Cancer Progression ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
Suppressor Gene ◽  
Sporadic Colorectal Cancer ◽  
Limited Information

Sporadic colorectal cancer (CRC) develops through distinct molecular events. Loss of 18q chromosome is a conspicuous event in the progression of adenoma to carcinoma. There is limited information regarding the molecular effectors of this event. Earlier, we had reported ATP8B1 as a novel gene associated with CRC. ATP8B1 belongs to the family of P-type ATPases (P4 ATPase) that primarily function to facilitate the translocation of phospholipids. In this study, we attempt to implicate ATP8B1 gene located on chromosome 18q as a tumor suppressor gene. We studied indigenous patient data and confirmed the reduced expression of ATP8B1 in tumor samples. CRC cell lines were engineered with reduced and enhanced levels of ATP8B1 which provided a tool to study its role on cancer progression. Forced reduction of ATP8B1 expression either by CRISPR/Cas9 or shRNA was associated with increased growth and proliferation of CRC cell line - HT29. In contrast, overexpression of ATP8B1 resulted in reduced growth and proliferation of SW480 cell line. We generated a network of genes that are downstream of ATP8B1. Further, we provide predicted effect of modulation of ATP8B1 levels on this network and possible effect on fatty acid metabolism related genes. These results provide evidence in support of ATP8B1 being a tumor suppressor that may affect fatty acid metabolism in CRC.

scholarly journals LYAR Promotes Colorectal Cancer Progression by Upregulating FSCN1 Expression and Fatty Acid Metabolism

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Yupeng Wu ◽  
Yu Zhou ◽  
Haiying Gao ◽  
Yajun Wang ◽  
Qingyu Cheng ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acid ◽  
Cancer Progression ◽  
Fatty Acid Metabolism ◽  
Molecular Mechanisms ◽  
Acid Metabolism ◽  
Migration And Invasion ◽  
Tumor Cell Migration ◽  
Gene Reporter Assay

Colorectal cancer (CRC) is a highly malignant tumor associated with poor prognosis, yet the molecular mechanisms are not fully understood. In this study, we showed that LYAR, a nucleolar protein, is expressed at a higher level in CRC tissue than in adjacent normal tissue and that LYAR expression is closely associated with distant CRC metastasis. LYAR not only significantly promotes the migration and invasion of CRC cells in vitro, but knockdown (KD) of LYAR in CRC cells also inhibits xenograft tumor metastasis in vivo. Microarray analysis of LYAR KD cells combined with a chromatin immunoprecipitation (ChIP) assay, gene reporter assay, and rescue experiment indicated that FSCN1 (encoding fascin actin-bundling protein 1 (Fascin-1)) serves as a novel key regulator of LYAR-promoted migration and invasion of CRC cells. Knockdown of FSCN1 significantly inhibits subcutaneous tumorigenesis of CRC cells and leads to the downregulation of FASN and SCD, genes encoding key enzymes in fatty acid synthesis. In summary, this study reveals a novel mechanism by which LYAR promotes tumor cell migration and invasion by upregulating FSCN1 expression and affecting fatty acid metabolism in CRC.

Polyunsaturated fatty acid metabolism in enterocyte models: T84 cell line vs. Caco-2 cell line

2013 ◽  
Vol 50 (2) ◽  
pp. 111-120 ◽  
Author(s):  
Pauline Beguin ◽  
Anne-Catherine Schneider ◽  
Eric Mignolet ◽  
Yves-Jacques Schneider ◽  
Yvan Larondelle
Keyword(s):  
Fatty Acid ◽  
Cell Line ◽  
Polyunsaturated Fatty Acid ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
T84 Cell Line

scholarly journals Oroxylin A suppresses the development and growth of colorectal cancer through reprogram of HIF1α-modulated fatty acid metabolism

2017 ◽  
Vol 8 (6) ◽  
pp. e2865-e2865 ◽  
Author(s):  
Ting Ni ◽  
Zihao He ◽  
Yuanyuan Dai ◽  
Jingyue Yao ◽  
Qinglong Guo ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acid ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
Oroxylin A

scholarly journals Reprogramming of fatty acid metabolism in cancer

2019 ◽  
Vol 122 (1) ◽  
pp. 4-22 ◽  
Author(s):  
Nikos Koundouros ◽  
George Poulogiannis
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Cancer Cells ◽  
Cancer Progression ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
De Novo ◽  
Great Promise ◽  
Molecular Heterogeneity

AbstractA common feature of cancer cells is their ability to rewire their metabolism to sustain the production of ATP and macromolecules needed for cell growth, division and survival. In particular, the importance of altered fatty acid metabolism in cancer has received renewed interest as, aside their principal role as structural components of the membrane matrix, they are important secondary messengers, and can also serve as fuel sources for energy production. In this review, we will examine the mechanisms through which cancer cells rewire their fatty acid metabolism with a focus on four main areas of research. (1) The role of de novo synthesis and exogenous uptake in the cellular pool of fatty acids. (2) The mechanisms through which molecular heterogeneity and oncogenic signal transduction pathways, such as PI3K–AKT–mTOR signalling, regulate fatty acid metabolism. (3) The role of fatty acids as essential mediators of cancer progression and metastasis, through remodelling of the tumour microenvironment. (4) Therapeutic strategies and considerations for successfully targeting fatty acid metabolism in cancer. Further research focusing on the complex interplay between oncogenic signalling and dysregulated fatty acid metabolism holds great promise to uncover novel metabolic vulnerabilities and improve the efficacy of targeted therapies.

scholarly journals Intake of red and processed meat, use of non-steroid anti-inflammatory drugs, genetic variants and risk of colorectal cancer; a prospective study of the Danish “Diet, Cancer and Health” cohort

2018 ◽  
Author(s):  
Vibeke Andersen ◽  
Ulrich Halekoh ◽  
Anne Tjønneland ◽  
Ulla Vogel ◽  
Tine Iskov Kopp
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acid ◽  
Fatty Acid Metabolism ◽  
Genetic Variants ◽  
Acid Metabolism ◽  
Processed Meat ◽  
Gene Variants ◽  
Meat Intake ◽  
Inflammatory Drugs ◽  
Red And Processed Meat

Red and processed meat have been associated with increased risk of colorectal cancer (CRC), whereas long-term use of non-steroid anti-inflammatory drugs (NSAIDs) may reduce the risk. The aim was to investigate potential interactions between meat intake, NSAID use, and gene variants in fatty acid metabolism and NSAID pathways in relation to the risk of CRC. A nested case-cohort study of 1038 CRC cases and 1857 randomly selected participants from the Danish prospective “Diet, Cancer and Health” study encompassing 57,053 persons was performed using the Cox proportional hazard models. Gene variants in SLC25A20, PRKAB1, LPCAT1, PLA2G4A, ALOX5, PTGER3, TP53, CCAT2, TCF7L2, BCL2 were investigated. CCAT2 rs6983267 was associated with risk of CRC per se (p<0.01). Statistically significant interactions were found between intake of red and processed meat and CCAT2 rs6983267, TP53 rs1042522, LPCAT1 rs7737692, SLC25A20 rs7623023 (pinteraction=0.04, 0.04, 0.02, 0.03, respectively), and use of NSAID and alcohol intake and TP53 rs1042522 (pinteraction=0.04, 0.04, respectively) in relation to risk of CRC. No other consistent associations or interactions were found. This study replicated an association of CCAT2 rs6983267 with CRC and an interaction between TP53 rs1042522 and NSAID in relation to CRC. Interactions between genetic variants in fatty acid metabolism and NSAID pathway and intake of red and processed meat were found. Our results suggest that meat intake and NSAID use affect the same carcinogenic mechanisms. All new findings should be sought replicated in independent prospective studies. Future studies on the cancer-protective effects of aspirin/NSAID should include gene and meat assessments.

Dynamics of fatty acid metabolism in a cell line from southern bluefin tuna (Thunnus maccoyii)

Aquaculture ◽  
2015 ◽  
Vol 449 ◽  
pp. 58-68 ◽  
Author(s):  
Andrew M. Scholefield ◽  
Douglas R. Tocher ◽  
Kathryn A. Schuller
Keyword(s):  
Fatty Acid ◽  
Cell Line ◽  
Fatty Acid Metabolism ◽  
Acid Metabolism ◽  
Bluefin Tuna ◽  
Southern Bluefin Tuna ◽  
A Cell
Sign in / Sign up

Export Citation Format

Share Document