scholarly journals Variability of Mnemiopsis leidyi A. Agassiz (Ctenophora: Lobata) bioluminescence in relation to regeneration

2016 ◽  
Vol 1 (1) ◽  
pp. 36-42
Author(s):  
O. V. Mashukova ◽  
Yu. N. Tokarev

Our previous works have been shown the light-emission of Mnemiopsis leidyi A. Agassiz, 1865 and Beroe ovata Mayer, 1912 is species-specific and variability of bioluminescence parameters (amplitude, energy and also light-emission duration) depending on temperature, food supply and other factors. Revealing of M. leidyi light-emission variability during their regeneration after impactof predaceous organism B. ovata was the aim of our investigations. Definition of ctenophores bioluminescence characteristics was carry out on the laboratory complex “Light”. The ctenophore bioluminescence was investigated by method  of mechanical and chemical stimulation. The ctenophores, which were seriously injured but preserved their statocyst intact, went on actively moving but the characteristics of their light-emission lessened to minimal – 17.08·108 quantum·s−1·cm−2 and 1.37 s under chemical stimulation, and 14.85·108 quantum·s−1·cm−2 and 1.25 s under the mechanical impact, respectively. Having completed regeneration and restored the body weight to the initial, the ctenophores maximally increased light emission – to (332.33 ± 16.61·108) quantum·s−1·cm−2 under the chemical stimulation and to (219.45 ± 10.97·108) quantum·s−1·cm−2 under the mechanical excitation. Assumptions of the factors having an impact on M. leidyi regeneration intensity and their luminescence variability of throughout this process are made. Consequently, the possibility of bioluminescence applying for species ecology specification is shown.

2020 ◽  
Vol 37 ◽  
pp. 19-26
Author(s):  
Olga Mashukova ◽  
Olga Danilova ◽  
Lidia Melnik

The purpose of the current studies is identification of the bioluminescence variability of the ctenophores Mnemiopsis leidyi in the process of regeneration. It has been stated that the ctenophores M. leidyi being seriously injured with preserved statocyst still actively move but the amplitude and duration of their bioluminescence lessen to their minimums that is 17.08 quantum·s-1·cm-2 and 1.37 s respectively under chemical stimulation and 14.85·108 quantum·s-1·cm-2 and 1.25 s respectively under the mechanical impact. Having completed regeneration and restored the body weight up to the initial value, the ctenophores increased their light emission up to the maximum levels corresponding to 332.33 ± 16.61∙108 quantum·s-1·cm-2 under the chemical stimulation and to 219.45 ± 10.97∙108 quantum·s-1·cm-2 under the mechanical impact respectively. Several assumptions identifying the factors influencing the regeneration rate of the ctenophores M. leidyi and their bioluminescence variability range during their regeneration have been made. The possibility of applying bioluminescence for detecting ecological features associated with particular species is demonstrated.


2017 ◽  
Vol 14 ◽  
pp. 109-118
Author(s):  
Olga Mashukova ◽  
Yuriy Tokarev ◽  
Ekaterina Skuratovskaya

Investigations were conducted in the Department of biophysical ecology of Kovalevsky IMBR of RAS in September - October of 2013 and 2015. The body length of the gathered for experiments ctenophores was 35 – 40 mm. Characteristics of the ctenophores light emission were studied under the mechanical and chemical stimulations, with the usage of laboratory complex “Svet”. The following HM salts: Cu2S04, ZnCl2, PbCl2 and HgCl2 in different concentrations were used in our experiments. The just-caught samples, contained in the clean marine water were used as a control. The exposition time was 1, 3 and 24 hours under the temperature of 21 ± 2°C. The investigations results have shown considerable variability of the ctenophore luminosity characteristics in dependence of metal concentration and exposition duration. It was stated that minimal concentrations of cooper, zinc and mercury stimulates ctenophores bioluminescence and the high ones inhibit. The alien ctenophore luminescence inhibition was registered under the lead activity under all investigated concentrations. We can place investigated metals as following: Zn < Cu < Hg < Pb, according to the force of the toxic influence on the ctenophore bioluminescence. Thus, alien ctenophore bioluminescence parameters can serve as a sensitive express-indicator of the resistance degree to the heavy metals impact and be the expressive index of the marine environment regional pollution.


Author(s):  
Dan Claudiu ROŞCA ◽  
Marius Giorgi USTUROI

Growing guinea-fowls is an area of less research activity, although meat and eggs from this species are increasingly sought by consumers around the world. In addition, there is a number of very valuable biological features that makes this sector such attractive. This paper deals with a study on the productive performance of the gray guinea fowl under controlled ambient conditions. The biological material consisted of 50 specimens of guinea-fowls, purchased from a producer in our country. Those guinea fowls were grown according to the species-specific technology, from one day to 77 days. The target indicators were body weight, weight gain, feed consumption and stockholding proportion, determined in accordance with the poultry research methodology. The data were statistically processed using the Anova (Analysis of variance) program. For the gray guinea fowl, the growing period has been divided into three stages of age, differentiated by the protein level of the feed. At the time of population, the body weight of birds was 28.09 ± 0.35 g, and on the day of slaughter (77th day), 1923.76 ± 18.29 g. This weight was achieved with a feed conversion index of 1: 3.64.The gray guinea fowl exhibits good organic resistance and ensures satisfactory meat production under conditions of optimal nutrient feeding.


2014 ◽  
Vol 84 (1-2) ◽  
pp. 5-11 ◽  
Author(s):  
Eun Y. Jung ◽  
Sung C. Jun ◽  
Un J. Chang ◽  
Hyung J. Suh

Previously, we have found that the addition of L-ascorbic acid to chitosan enhanced the reduction in body weight gain in guinea pigs fed a high-fat diet. We hypothesized that the addition of L-ascorbic acid to chitosan would accelerate the reduction of body weight in humans, similar to the animal model. Overweight subjects administered chitosan with or without L-ascorbic acid for 8 weeks, were assigned to three groups: Control group (N = 26, placebo, vehicle only), Chito group (N = 27, 3 g/day chitosan), and Chito-vita group (N = 27, 3 g/day chitosan plus 2 g/day L-ascorbic acid). The body weights and body mass index (BMI) of the Chito and Chito-vita groups decreased significantly (p < 0.05) compared to the Control group. The BMI of the Chito-vita group decreased significantly compared to the Chito group (Chito: -1.0 kg/m2 vs. Chito-vita: -1.6 kg/m2, p < 0.05). The results showed that the chitosan enhanced reduction of body weight and BMI was accentuated by the addition of L-ascorbic acid. The fat mass, percentage body fat, body circumference, and skinfold thickness in the Chito and Chito-vita groups decreased more than the Control group; however, these parameters were not significantly different between the three groups. Chitosan combined with L-ascorbic acid may be useful for controlling body weight.


Derrida Today ◽  
2013 ◽  
Vol 6 (1) ◽  
pp. 97-114 ◽  
Author(s):  
Christopher Morris

Over the past thirty years, academic debate over pornography in the discourses of feminism and cultural studies has foundered on questions of the performative and of the word's definition. In the polylogue of Droit de regards, pornography is defined as la mise en vente that is taking place in the act of exegesis in progress. (Wills's idiomatic English translation includes an ‘it’ that is absent in the French original). The definition in Droit de regards alludes to the word's etymology (writing by or about prostitutes) but leaves the referent of the ‘sale’ suspended. Pornography as la mise en vente boldly restates the necessary iterability of the sign and anticipates two of Derrida's late arguments: that there is no ‘the’ body and that performatives may be powerless. Deriving a definition of pornography from a truncated etymology exemplifies the prosthesis of origin and challenges other critical discourses to explain how pornography can be understood as anything more than ‘putting (it) up for sale’.


2020 ◽  
Vol 99 (6) ◽  
pp. 271-276

Introduction: Prevalence of obesity is 30 % in the Czech Republic and is expected to increase further in the future. This disease complicates surgical procedures but also the postoperative period. The aim of our paper is to present the surgical technique called hand-assisted laparoscopic nephrectomy (HALS), used in surgical management of kidney cancer in morbid obese patients with BMI >40 kg/m2. Methods: The basic cohort of seven patients with BMI >40 undergoing HALS nephrectomy was retrospectively evaluated. Demographic data were analyzed (age, gender, body weight, height, BMI and comorbidities). The perioperative course (surgery time, blood loss, ICU time, hospital stay and early complications), tumor characteristics (histology, TNM classification, tumor size, removed kidney size) and postoperative follow-up were evaluated. Results: The patient age was 38−67 years; the cohort included 2 females and 5 males, the body weight was 117−155 kg and the BMI was 40.3−501 kg/m2. Surgery time was 73−98 minutes, blood loss was 20−450 ml, and hospital stay was 5−7 days; incisional hernia occurred in one patient. Kidney cancer was confirmed in all cases, 48–110 mm in diameter, and the largest removed specimen size was 210×140×130 mm. One patient died just 9 months after the surgery because of metastatic disease; the tumor-free period in the other patients currently varies between 1 and 5 years. Conclusion: HALS nephrectomy seems to be a suitable and safe surgical technique in complicated patients like these morbid obese patients. HALS nephrectomy provides acceptable surgical and oncological results.


Author(s):  
E.P. Dolgov ◽  
◽  
A.A. Abramov ◽  
E.V. Kuzminova ◽  
E.V. Rogaleva ◽  
...  

The article presents the data on the study of the influence of mycotoxins combination (T-2 toxin at the concentration of 0.095 mg/kg and aflatoxin B1 in the concentration of 0.019 mg/kg) on the body of quails and the results of pharmacocorrection of toxicosis with a complex consisting of beet pulp and lecithin. Structural changes in the intestines of quais at fodder mycotoxicosis are described. The use of antitoxic feed additives in poultry led to a weakening of the action of xenobiotics, which was confirmed by an increase in the safety of poultry and increase in body weight of quails, a decrease in the clinical manifestations of intoxication, as well as in positive changes in the structure of the intestine of the poultry during histological examination.


Author(s):  
Hawraa M. Murad ◽  
Tamadhur Hani Hussein ◽  
Audai Sulaiman Khudhair ◽  
Manal Muhi Murad ◽  
Jawad Kadhim Faris

This study was conducted to find out hepatoprotective activity of hesperidin (HES) 100mg/kg body weight (b.w.) against ciprofloxacin (CPX) 100 mg/kg induced hepatotoxicity in local breed rabbits .CPX is a broad spectrum antibiotic used for treatment of many bacterial infections. Twenty four male rabbits were divided into four groups ,group1: control, (1 ml/kg Saline orally) group 2: CPX (100 mg/kg orally) for (14) consecutive days , group 3: HES (100 mg//kg) orally for (14) consecutive days group 4: CPX (100 mg/kg orally) plus HES (100 mg//kg orally ) for (14) consecutive days. All the rabbits were killed on the (15) day of the experiment, and then the blood, and livers samples were taken. CPX induced hepatotoxicity was proved by a significant (p less than 0.01) reduction in the body weight ,and a significant (p less than 0.01) increased serum aspartate transaminase (AST), alanine transaminase (ALT) , Malonaldehyde enzyme (MAD) and histopathological changes. Protective hepatic toxicity effect and oxidative damage caused by CPX significantly (p less than 0.01) increasing in body weight and significantly (p less than 0.01) decreasing AST , ALT, MAD and improving tissue morphology in HES (100 mg//kg) . These results assure that HES (100 mg//kg) antioxidant effects can protect CPX-induced hepatotoxicity in rabbits.


GYNECOLOGY ◽  
2018 ◽  
Vol 20 (2) ◽  
pp. 9-13
Author(s):  
A R Khachaturian ◽  
E V Misharina ◽  
M I Yarmolinskaya

Androgen-dependent dermopathy, as well as premenstrual syndrome of varying severity in young women, can cause emotional depression, difficulties in social adaptation and even depressive disorders. The aim of the study was to study the safety and efficacy of using a combined oral contraceptive (COC) Dimia® containing 20 μg ethinyl estradiol and 3 mg drospirenone in young women, as well as its therapeutic effects in androgen-dependent dermopathy. Materials and methods. The study included 57 young women aged 23.1±2.2 years with signs of androgen-dependent dermopathy. The evaluation of the change in the character of menstrual bleeding, the anthropometric parameters (body weight, waist circumference and hips), the therapeutic effect of the drug on the symptoms of androgen-dependent dermopathy, as well as the dynamics of arterial pressure, hemoglobin level, serum iron have been studied. The psycho-emotional state was assessed using the SAN questionnaire (well-being-activity-mood). Results. During 6 months of observation, there was no significant change in the body mass index, waist circumference, and hips, and the drug did not affect the blood pressure numbers. Against the background of taking the drug, there was an increase in the parameters of iron metabolism (hemoglobin content, serum iron). After 3 months of taking the contraceptive with drospirenone, the number of patients with a complaint about the abundance of menstruation decreased more than twofold (from 22.8 to 10.5%), and after 6 months of taking the drug no patient noted the profuse nature of menstruation. Before the start of taking COC with drospirenone, 57.9% of women reported painful menstrual bleeding. Against the background of taking the contraceptive within 3 months, this complaint was stopped in all patients. Sufficient efficacy of treatment of androgen dependent dermopathy in young women with the help of a microdosed drospirenone-containing combined oral contraceptive is estimated from the dermatological acne index. The analysis of the SAN questionnaire made it possible to reveal the improvement in the psychoemotional state of patients on the background of taking the drug. The conclusion. The results obtained proved the effectiveness and safety of the microclinized COC Dimia®. The drug has no significant effect on body weight, blood pressure, provides reliable control of the cycle and a decrease in menstrual bleeding, which results in stabilization of iron metabolism in the body. Dimia® is effective in the treatment of androgen-dependent dermopathy and can be recommended to young women for starting contraception.


2018 ◽  
Vol 12 (2) ◽  
pp. 75-84
Author(s):  
M.V. Arisov ◽  
I.P. Belykh ◽  
V.V. Artemov

The purpose of the research: the study of the efficacy of the preparations for veterinary use "Inspector Quadro C" and "Inspector Quadro K" against ecto- and endoparasitoses of dogs and cats. Materials and methods. Studies were conducted on spontaneously infected dogs and cats of different sexes, age, weight and breed. The diagnosis of infection with ectoparasites was made based on the clinical picture and laboratory methods of investigation (microscopy of scrapings taken from ectoparasitized skin areas, examination of the coat for fleas, lice, worms, ixodids). Infection with helminths was established by detecting eggs of helminths in faeces of animals by the method of Füleleborn and mature segments of cestodes. Preparations were applied to the animals by drip application on dry undamaged skin in places inaccessible to licking in a dose of 0.1-0.4 ml per 1 kg of body weight. The results were statistically processed. Results and discussion. "Inspector Quadro S" and "Inspector Quadro K" showed 100% efficacy at sarcoptosis in dogs, notoedrosis in cats, otodectosis in dogs and cats, ixodidoses and entomoses. "Inspector Quadro C" showed a high efficiency (92.3%) at demodecosis in dogs. However, single mites were found in one dog. 100% efficacy of "Inspector Quadro C" and "Inspector Quadro K" has been established against intestinal nematodes and cestodes in dogs and cats. Negative effects of drugs on the body of animals have not been revealed.


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