scholarly journals AKTIVITAS ANTIBAKTERI DARI DESAIN MIKROEMULSI MINYAK ATSIRI KAYU MANIS

AGROINTEK ◽  
2017 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Evelyn Djiuardi ◽  
Tutun Nugraha
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Carboxymethyl Cellulose ◽  
Pathogenic Bacteria ◽  
Tween 80 ◽  
Tween 20 ◽  
Oil In Water ◽  
Cinnamon Essential Oil

<span class="fontstyle0">This research focused on the study of the capability of cinnamon essential oil as antibacterial<br />agent, when it is made as a microemulsion solution. The study occupied further research on<br />specific type, concentration, and amount of emulsifier needed to make the cinnamon essential<br />oil miscible in water without causing destruction in its antibacterial activity. Four different<br />emulsifiers were tested, namely Tween 20, Tween 80, soya lechitin, and carboxymethyl cellulose<br />(CMC) to stabilize the essential oil in water. The emulsifiers were used at 2% w/w, while the<br />essential oil concentrations were varied at 0.5%, 1%, and 1.5%. In this study, the antibacterial<br />activity of the microemulsion solution of cinnamon essential oil was tested against two types of<br />pathogenic bacteria commonly found in foods, namely Staphylococcus aureus and Escherichia<br />coli. Among the four types of emulsifiers, which are used, CMC showed the best results as an<br />emulsifier. The results of this study indicated that microemulsion solution has antibacterial<br />activity best is a combination between the CMC and the essential oil of cinnamon</span>

scholarly journals Antibacterial Activity of Endostemon tereticaulis (Poir.) M. Ashby Essential Oil and Ethanolic Extract Against Resistant Pathogenic Bacteria

2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2095325
Author(s):  
Bala Namata Abba ◽  
Abderrahmane Romane ◽  
Amadou Tidjani Ilagouma
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Traditional Medicine ◽  
Pathogenic Bacteria ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Minimal Bactericidal Concentration

Endostemon tereticaulis (poir.) M.Ashby is a species of the Lamiaceae family present in Niger. This plant is used in traditional medicine due to its various biological potentialities. The present study investigated the chemical composition of the essential oil and the antibacterial activity of the essential oil and ethanolic extract of Endostemon tereticaulis against resistant pathogenic bacteria. Gas chromatography-mass spectrometry analysis of the essential oil led to the identification of 43 compounds representing 99.55% of the total essential oil. The major components were caryophyllene oxide (15.17%) followed by α-humulene (13.96%), α-copaene (11.75%), ( E)-β-caryophyllene (8.44%), and δ-cadinene (6.78%). The antibacterial activity was tested against multiresistant Acinetobacter baumannii P1483, Salmonella spp. H1548, extended-spectrum β-lactamase- Escherichia coli Bu8566, Enterobacter cloacae Bu147, Proteus mirabilis Bu190 , Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25922), Enterococcus faecium H3434, methicillin-resistant Staphylococcus aureus P1123, and Staphylococcus aureus (ATCC 25923). The antibacterial assays revealed that the essential oil was more active than the ethanolic extract against the studied bacteria with minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values ranging from 0.06 to 2 mg/mL. Also, the ethanolic extract was effective against the bacteria tested with MIC and MBC values ranging from 0.12 to 3 mg/mL. This study showed that Endostemon tereticaulis essential oil is rich in bioactive compounds. Ethanolic extract and essential oil exhibited potential antibacterial activity. These results provide a scientific basis for the use of this plant in traditional medicine. The current study described for the first time the antibacterial activity of Endostemon tereticaulis.

scholarly journals Chemical composition, antibacterial activity of essential oil and anatomical study of Chrysanthemum morifolium

2020 ◽  
Vol 10 (2-s) ◽  
pp. 7-13
Author(s):  
Habiba BOUKHEBTI ◽  
I. Demirtas ◽  
L. Omar ◽  
A.N. Chaker
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Anatomical Study ◽  
Chrysanthemum Morifolium ◽  
Citrobacter Freundii ◽  
Agar Diffusion Test

The aim of this study is to identify the chemical composition and to evaluate the antimicrobial activity of Chrysanthemum morifolium. The analysis and identification of essential oil which obtained by hydrodistilation method were realized by gaz chromatography and mass spectroscopy. The antibacterial activity was tested by using the agar diffusion test and the Gram positive and negative pathogenic bacteria: Staphylococcus aureus ATCC 25923. Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Citrobacter freundii ATCC 8090, Kleibseilla pneumoniae ATCC 700603 and Shigella sonnei were used to evaluate this activity. This analysis led to the identification of 26 compounds representing 88.40 % of the total essential oil mass. The major compound was Verbenone (17.33 %). Other components present in appreciable contents were: Chrysanthenone (9.71%). 4-epi-cubedol (07.25%) and δ-Cadinol (05.29 %). Essential oil of Chrysanthemum morifolium exhibited an antibacterial effect against pathogenic bacteria, like those observed against Staphylococcus aureus ATCC 25923 (35±1.2mm) and Citrobacter freundii ATCC 8090 (21±0.87mm), however Pseudomonas aeruginosa  ATCC  27853  and  Kleibseilla  pneumoniae  ATCC  700603                      were resistant. The anatomical study showed the presence of several types of trichomes including the glands secreted for essential oils and protector trichomes. Keywords: essential oil, antibacterial activity, Chrysanthemum morifolium, anatomical study, chemical composition

scholarly journals Daya Hambat Krim Minyak Atsiri Herba Boborangan (Hyptis brevipes) sebagai antibakteri terhadap Staphylococcus aureus

2021 ◽  
Vol 7 (1) ◽  
pp. 31-38
Author(s):  
Arviani - Arviani ◽  
Dwi Larasati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Promising Candidate ◽  
Water Emulsion ◽  
Oil In Water ◽  
Oil In Water Emulsion

Boborongan (Hyptis brevipes) is empirically used in ethnomedicine containing of essential oil which it is applied to inhibit the growth of the Staphyllococcus aureus. Cream preparation along with oil in water emulsion is often applied due to easiness and compliance particulary for  cosmetic and aesthetic use. This study aimed to determine the minimum inhibitory concentration of cream based essential oil of Hyptis brevipes against Staphylococcus aureus. Essential oil from Hyptis brevipes was isolated using the water vapor distillation method. Cream based essential oil of Hyptis brevipes of 0.1%, 0.2%, and 0.4%, respectively. Antibacterial activity study was carried out using a well diffusion method. Antibacterial inhibition was obtained by measuring the clear zone on the medium. The results of the antibacterial inhibition test showed that the cream based Hyptis brevipes essential oil along with concentration of 0.1%, 0.2%, and 0.4% showed antibacterial activity with the diameter of inhibition zone are 5.3 mm; 5.1 mm; and 6 mm, respectively. Therefore, it prooved that the essential oil of Hyptis brevipes was a promising candidate for antibacterial substance

scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil of Lasiocephalus longipenicillatus (Senecio longipenicillatus)

2006 ◽  
Vol 1 (2) ◽  
pp. 1934578X0600100 ◽  
Author(s):  
María Rondón ◽  
María Araque ◽  
Antonio Morales ◽  
María Gualtieri ◽  
Janne Rojas ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Enterococcus Faecalis ◽  
Pathogenic Bacteria ◽  
Human Pathogenic Bacteria ◽  
Germacrene D

The analysis of the essential oil of Lasiocephalus longipenicillatus by GC-MS, yielded α-pinene (48.3%), α-humulene (15.8%) and germacrene-D (15.5%) as major constituents. The essential oil showed a strong antibacterial activity against important human pathogenic bacteria, such as Staphylococcus aureus and Enterococcus faecalis.

scholarly journals Essential Oil Analysis and Antibacterial Activity of Rosmarinus tournefortii from Algeria

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Mansouria Souria Bendeddouche ◽  
Hachemi Benhassaini ◽  
Zouaoui Hazem ◽  
Abderrahmane Romane
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Oil Analysis ◽  
Gram Negative ◽  
E Coli ◽  
Aerial Parts

The volatile compounds obtained by hydrodistillation of the aerial parts of Rosmarinus tournefortii De Noé. growing wild in the occidental region of Algeria were analyzed by GC/MS. Thirty-six compounds were characterized representing 95.6% of the essential oil, with camphor (37.6%), 1,8-cineole (10.0%), p-cymene-7-ol (7.8%), and borneol (5.4%) as the major components. The antimicrobial activity was evaluated against three pathogenic bacteria: Gram-negative ( Escherichia coli and Pseudomonas aeruginosa) and Gram-positive ( Staphylococcus aureus). The minimum inhibitory concentration (MIC; mg/mL) was determined by sub-culture on Muller Hinton agar plates. The essential oil exhibited strong antibacterial activity against E. coli and P. aeruginosa, and was also active against Staphylococcus aureus.

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

scholarly journals Preliminary phytochemical screening and susceptibility of bacteria pathogens to whole extract of evolvulus alsinoides (L.)

1970 ◽  
Vol 18 ◽  
pp. 16-20
Author(s):  
BA Omogbai ◽  
FA Eze
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Volatile Oil ◽  
Salmonella Typhi ◽  
Phytochemical Screening ◽  
Evolvulus Alsinoides ◽  
And Staphylococcus Aureus

Context: Plant based antimicrobial represent a vast untapped source for medicines and further exploration of plant antimicrobial neeto occur. Evolvulus alsinoides (L) (Convolvulaceae) is a perennial herb is used in traditional medicine in East Asia, India, Africa and Philippines to cure fever, cough, cold, venereal diseases, azoospermia, adenitis and dementia.   Objective: The objective of this research was to evaluate the antimicrobial activity of the extracts of E. alsinoides on some clinical microbial isolates.   Materials and Methods: The ed thanolic and aqueous extracts of the whole plant (leaves and twigs) were analysed for alkanoids, tannins, glycosides, steroids, flavonoids, saponins, volatile oil and resins. The determination of antibacterial activity was done using the agar well diffusion technique. Pure cultures of pathogenic bacteria such as Bacillus cereus, Staphylococcus aureus, Micrococcus leutus, Klebsiella Pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi were used for antibacterial activity assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).   Results: The ethanolic extract of the plant had MIC values ranging from 16 mg/ml to 512.5 mg/ml. The least MIC was 16mg-ml against Salmonella typhi while Bacillus cereus and Staphylococcus aureus showed the highest MIC of 512.5 mg-ml. In the aqueous extract the MIC ranged between 512.5 to >1025 mg/ml. Salmonella typhi, Micrococcus luteus and Staphylococcus aureus were not inhibited by the water extract. Phytochemical result showed ethanol to be a better solvent for the extraction of the bioactive agents in this plant which include: glycosides, alkaloids, saponins, tannins, flavonoids and volatile oil.   Conclusion: In this study the gram-negative organisms had the lowest MICs and MBCs. This suggests their higher susceptibility to the extract of this plant. On the basis of the result obtained in this investigation it can be concluded that ethanol extract of Evolvulus alsinoides had significant in vitro broad spectrum antimicrobial activity.   Keywords: Evolvulus alsinoides; Phytochemical screening; Antibacterial activity. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8769 JBS 2010; 18(0): 16-20

scholarly journals Antibacterial activity of ginger extracts and its essential oil on some of pathogenic bacteria

2010 ◽  
Vol 7 (3) ◽  
pp. 1159-1165
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Cold Water ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Hot Water ◽  
Diffusion Method ◽  
Resistant Bacteria

The antimicrobial activity of ginger extracts ( cold-water, hot-water, ethanolic and essential oil ) against some of pathogenic bacteria ( Escherichia coli , Salmonella sp , Klebsiella sp , Serratia marcescens, Vibrio cholerae , Staphylococcus aureus , Streptococcus sp) was investigated using Disc diffusion method , and the results were compared with the antimicrobial activity of 12 antibiotics on the same bacteria . The results showed that the ginger extracts were more effective on gram-positive bacteria than gram-negative . V. cholerae and S. marcescens,were the most resistant bacteria to the extracts used , while highest inhibition was noticed against Streptococcus sp (28 mm) . The ethanolic extract showed the broadest antibacterial activity ( 11 to 28 mm ) , in comparison with moderate activity of essential oil , it was observed that the cold-water extract was more effective on the bacteria than hot-water extract . Ginger ethanolic extract presented higher diameter of inhibition zone for Streptococcus sp than in Ciprofloxacin , Cefotaxime , Cefalotin , Cephalexin and Cephaloridine , also it was found a similarity between the higher inhibition zones of ethanolic extract of ginger and some antibiotics for S. aureus , E. coli , Salmonella sp and Klebsiella sp . V. cholerae and S. marcescens,also highly resistant to antibiotics . Phytochemical analysis of ethanolic extract of ginger revealed the present of glycosides, terpenoids, flavonids and phenolic compounds

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