scholarly journals 9. Total colony of cellulolitic bacteria in the rumen of aceh cattle

2017 ◽  
Vol 11 (1) ◽  
pp. 51-58
Author(s):  
Safika Safika ◽  
Siti Wardianah Matondang ◽  
Darmawi Darmawi ◽  
Mahdi Abral ◽  
Erina Erina ◽  
...  
Keyword(s):  
Petri Dish ◽  
Colony Morphology ◽  
Plate Method ◽  
Gram Positive ◽  
Gram Staining ◽  
Colony Counting ◽  
Total Bacteria ◽  
Banda Aceh

The aim of this study was to count the total sum of cellulolitic bacteria colony in  aceh cattle rumen. The sample used was aceh cattle rumen liquid which taken from salughtered house in Banda Aceh. Aceh cattle rumen liquid were diluted from 10-2 to 10-5, then inoculated to agar BHM-CMC media with pour plate method. Petri dish was entered to candle jar to be incubated in an incubator with temperature 37oC about 96 hours. Colony of cellulolitic bacteria which were grown then were observed by using colony bacteria criteria (colour, position, edge, and diameter of colony), counting total of bacteria which was grown, and Gram staining. The results showed that there is a white translucent colony morphology, growth inside agar, smooth periphery, Gram-positive bacil. The average number bacteria of total bacteria in the cattle rumen liquid was 4,81 x 105 cfu/ml

scholarly journals Identifikasi Staphylococcus aureus Pada Abon Sapi Di Pasar Pahing Kota Kediri

2019 ◽  
Vol 5 (2) ◽  
pp. 99-105
Author(s):  
Mastuti Widianingsih ◽  
Dian Catur Setyorini
Keyword(s):  
Staphylococcus Aureus ◽  
Gastrointestinal Tract ◽  
Manufacturing Process ◽  
Sampling Technique ◽  
Colony Morphology ◽  
Positive Staining ◽  
Dirty Hands ◽  
Gram Positive ◽  
Gram Staining ◽  
Fermentation Test

Contamination of processed beef foods such as abon can be caused by various types of microbes, one of which is Staphylococcus aureus. Staphylococcus aureus can cause various infections, both on the skin, gastrointestinal tract, or endocarditis. The objective of this research was to determine the presence of Staphylococcus aureus in beef abon sold in Pahing Market, Kediri. Abon used is non-branded beef abon which is as many as 10 samples obtained by total sampling technique. Samples were tested by observation of colony morphology through Gram staining, mannitol fermentation test, catalase and coagulase test, and acetoin test. The samples were inoculated on Broth NaCl (ink. 24 hour-37°C), then inoculated on MSA (ink. 24 hour-37°C), and VP (ink. 2x24 hours-37°C). Catalase and coagulase tests were carried out by taking colonies on MSA media. The results showed that there were 9 abon samples contaminated with Staphylococcus aureus as indicated by Gram positive staining results, positive (perfect) mannitol fermentation, and positive acetoin, catalase, and coagulase test. The causes of contamination are contaminated abon ingredients, the manufacturing process using less sterile tools, poor handling and processing, processing food with dirty hands, food stored without cover, sick food processors, and dirty markets

scholarly journals ISOLASI BAKTERI COCCUS GRAM POSITIF DI DALAM SUSU ULTRA HIGH TEMPERATURE (UHT) 6 DAN 3 BULAN MENJELANG KEDALUWARSA (Isolation of Gram-Positive Cocci Bacteria in Ultra High Temperature (UHT) Processed Milk at 6 and 3 Months Prior to Expiration)

2016 ◽  
Vol 10 (1) ◽  
pp. 48 ◽  
Author(s):  
Dedy Sahputra ◽  
T. Reza Ferasyi ◽  
Ismail Ismail ◽  
Razali Razali ◽  
Sulasmi Sulasmi ◽  
...  
Keyword(s):  
High Temperature ◽  
Staining Method ◽  
Plate Method ◽  
Gram Positive ◽  
Uht Milk ◽  
Gram Positive Bacteria ◽  
Milk Samples ◽  
Gram Staining ◽  
Gram Positive Cocci ◽  
Ultra High Temperature

This research was aimed to isolate the Gram-positive bacteria that contaminated milk processed with ultra high temperature (UHT) at 6 and 3 months before the date expires. A number of 3 different UHT milk products at 6 and 3 months before expire date were used as sample. Milk samples were taken from several supermarket in Banda Aceh. The identification was conducted using Gram staining method. One ml milk sample was transferred into 18 tubes containing nutrient broth (NB) media and incubated at 37 C for 24 hrs. The sample was then culture on 18 petri discs containing manitol salt agar (MSA) and 18 petri discs containing trypticase soy sucrose bacitracin (TYS20B) using streak plate method. After 2 days, bacterial colonies that grow were identified by Gram staining. The results showed that in UHT processed milk at 6 and 3 prior to expiration contaminated with Gram positive cocci bacteria, which comprised of Staphylococcus aureus and Streptococcus mutant. As conclusion, UHT processed milk at 6 and 3 prior to expiration is contaminated by Gram positive cocci bacteria.

scholarly journals The Existence test of Salmonella thypii and Eschericia coli of Corn Ice at Padang Bulan’s Traditional Market, Medan

2020 ◽  
Vol 6 (1) ◽  
pp. 96-102
Author(s):  
Toberni S. Situmorang
Keyword(s):  
Plate Count ◽  
Gram Negative Bacteria ◽  
Food Hygiene ◽  
E Coli ◽  
Gram Staining ◽  
Hygiene Factors ◽  
Existence Test ◽  
Descriptive Method ◽  
Total Bacteria

Food hygiene is an important factor to protect ourselves from the contamination of germs and bacteria that enter through these food intermediaries. Food contaminated by bacteria will cause disease and can cause death if not treated immediately. Common bacteria that contaminate food are Salmonella thypii and Eschericia coli. Both types of bacteria are a group of gram-negative bacteria in the form of bacilli. This study aims to analyze and identify the bacteria S. thypii and E. coli found in corn ice samples. The study was conducted with a descriptive method by conducting a bacteriological examination to determine the quality of sample cleanliness. There are 3 stages in this study, the first stage is calculation of amount of total bacteria (total plate count), analysis of S. thypii and E. coli bacteria and identification with Gram staining. The results showed 40% of samples tested is positive for S. thypii and 60% for E. coli. The largest amount of total bacteria was shown by samples 1 and 3, which were 78 cfu and 52 cfu, respectively. The presence of S. thypii and E. coli bacteria in the sample is thought to be due to poor hygiene factors in the processing of the drink

scholarly journals Morphological characterization of gram-positive and gram-negative bacteria from treated latex processing wastewater

2021 ◽  
Vol 1 (2) ◽  
pp. 32-36
Author(s):  
Farrah Aini Dahalan ◽  
Nor Azizah Parmin
Keyword(s):  
Oxygen Demand ◽  
Morphological Characterization ◽  
The Other ◽  
Morphological Properties ◽  
Morphological Observation ◽  
Gram Positive ◽  
Bacterial Colony ◽  
Gram Negative ◽  
Gram Staining

A preliminary morphological screening and isolation of bacterial colony from latex industrial wastewater was carried out. Bacteria colonies from latex processing wastewater were isolated from a local latex processing industry. It was found that 17 bacterial isolates had been purified grown on nutrient agar under 35˚C. The colonies were then purified and morphologically indicated via Gram staining and motility test. After morphological observation, it was identified that out of 17 isolates, 9 isolates were Gram positive and 8 isolates were Gram negative. There are 11 out of 17 colonies were rod-shaped bacterial colonies, while the other 6 colonies were cocci-shaped bacteria. There were 11 colonies of gliding bacteria, three colonies were non-motile bacteria and the other three colonies were flagellated bacteria. This study is only limited to morphological observation as the main aim of this study was to investigate the potential occurrence of viable growth in treated latex processing wastewater. The bacterial colonies were classified base on their morphological properties shown. This study has classified several genera such as Staphylococcus, Escherichia, Thiobacillus, Arthrobacter and other Genus. The growth curve of 17 isolates studied and the chemical oxygen demand were determined.

Lysozyme Treatment Makes 16S rRNA Amplicon Sequencing Results Less Biased

2021 ◽  
Author(s):  
Yuting Jiao ◽  
Zijie Gao ◽  
Shiyu Gui ◽  
Lu Ren ◽  
Yongyue Lu ◽  
...  
Keyword(s):  
Structure Analysis ◽  
Community Analysis ◽  
Amplicon Sequencing ◽  
Microbial Community Analysis ◽  
Effective Agent ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
16S Rrna Amplicon Sequencing ◽  
Structure Result ◽  
Total Bacteria

Abstract Background Amplicon sequencing is widely applied in gut bacteria structure analysis. However, the proportion of Gram-positive bacteria may greatly affect the results of microbial community analysis. Lysozyme is an effective agent to extract DNA of Gram-positive bacteria. In this study, we assessed the influence of lysozyme treatment on results of Bactrocere dorsalis rectal bacteria structure. Result The results indicated that the total bacteria content can be significantly increased in lysozyme treated samples. Moreover, rectal bacteria diversity was significantly higher in lysozyme treated samples. A detail analysis revealed that abundance of Gram-positive bacteria significantly increased in samples treated with lysozyme. Conclusion This study indicates that lysozyme treatment before DNA extraction is an effective way to reduce bias in bacteria structure analysis, especially for samples with high proportion of Gram-positive bacteria.

The Biology of Bacteria

2019 ◽  
Author(s):  
Armine Sefton
Keyword(s):  
Cell Wall ◽  
Bacterial Infections ◽  
Genetic Material ◽  
Gram Positive ◽  
Dna And Rna ◽  
Gram Staining ◽  
Genetic Features ◽  
Potential Pathogen ◽  
A Cell ◽  
Micro Organisms

Bacterial infections and infestations of man can be caused by both microbes and non-microbes. Microbes include bacteria, viruses, fungi, and protozoa. Non-microbes include worms, insects, and arachnids. This chapter concentrates on the basic biology of bacteria. A pathogen is an organism that is able to cause disease in its host and the pathogenicity of any organism is its ability to produce disease. Microbes express their pathogenicity by means of their virulence. The virulence of any pathogen is determined by any of its structural, biochemical, or genetic features that enable it to cause disease in the host. The relationship between a host and a potential pathogen is non- static; the likelihood of any pathogen causing disease in its host depends both on the virulence of the pathogen and the degree of resistance or susceptibility of the host, due mainly to the effectiveness of the host’s defence mechanisms. Two of the main factors influencing a bacteria’s pathogenicity are its ability to invade and it ability to produce toxins—either exotoxins or endotoxins. Bacteria are unicellular prokaryotic micro-organisms, unlike human cells, which are eukaryotic. Fungi, protozoa, helminths, and arthropods are also eukaryotic. Prokaryotic organisms contain both DNA and RNA, but their genetic material exists unbound in the cytoplasm of the cell as, unlike eukaryotic cells, they have no nuclear membrane. Sometimes bacteria contain additional smaller circular DNA molecules, called plasmids. The main features of a bacterium are the cell wall, cytoplasm, and cell membrane. However, some bacteria have additional features such as spores, capsules, fimbriae (pili), and flagellae. The construction of the cell wall is different in different bacteria, but all cell walls contain peptidoglycan. The structure of the cell wall determines the staining characteristics when stained using the Gram stain. Although its first use was over a hundred and fifty years ago, is still the standard method for primary classification of bacteria. Occasionally, bacteria do not have a cell wall. Gram staining of a fixed smear of bacteria is used to separate bacteria into Gram positive or Gram negative, and also to demonstrate their shape. Bacteria with a thick peptidoglycan layer but with no outer membrane stain purple and are called Gram positive.

Pseudoepidemic of Endocarditis in Patients Undergoing Open Heart Surgery

1980 ◽  
Vol 1 (2) ◽  
pp. 97-99 ◽  
Author(s):  
Robert C. Aber ◽  
Peter C. Appelbaum
Keyword(s):  
Heart Valve ◽  
Heart Surgery ◽  
Open Heart Surgery ◽  
Careful Analysis ◽  
Screening Procedure ◽  
Gram Positive ◽  
Replacement Surgery ◽  
Usual Procedure ◽  
Gram Staining ◽  
Gram Positive Cocci

AbstractBetween July 2 and July 16, 1979, three patients were reported to have gram-positive cocci as seen on Gram stain of excised, macerated heart valve tissue. No organisms were isolated in culture; however, our usual procedure for handling such valves is not adequate for isolation of strict anaerobes. The first patient was treated for presumed endocarditis; this resulted in three additional weeks of hospitalization.Review of the remaining 34 patients who had heart valve replacement surgery between February 1 and July 20, 1979 revealed three additional cases.Careful analysis revealed that nonviable, gram-positive cocci present in commercially prepared trypticase soy broth were being added to the tissue just prior to mortar and pestle grinding in the microbiology laboratory. The contaminated tubes were noted to have a visible sediment when carefully examined.The procedure for handling the broth has been revised; a simple screening procedure is used now to identify potentially contaminated broth. No further instances of false positive results on Gram-staining of heart value tissue have occurred since this screening procedure was introduced.

scholarly journals Karakterisasi dan Identifikasi Bakteri Enterobacteriaceae pada Telur Penyu Lekang (Lepidochelys olivacea) asal Lhok Pante Tibang, Banda Aceh

2019 ◽  
Vol 37 (1) ◽  
pp. 41
Author(s):  
Risa Nursanty *, Widya Sari, Safranita Risa Nursanty *, Widya Sari, Safranita ◽  
Widya Sari ◽  
Safranita Safranita
Keyword(s):  
Cell Shape ◽  
Bacterial Contamination ◽  
Isolation Method ◽  
Contributing Factors ◽  
Olive Ridley ◽  
Biochemical Tests ◽  
Lepidochelys Olivacea ◽  
Gram Staining ◽  
Olive Ridley Turtle ◽  
Banda Aceh

The population of olive ridley turtle (Lepidochelys olivacea) are currently thretened with extinction, among the contributing factors is the failure of turtle eggs to hatch. The condition can be caused by the presence of bacterial contamination, especially group Enterobacteriaceae. The purpose of this research was to isolate the group of Enterobacteriaceae bacteria from olive ridley turtle. The sample used were shells from olive ridley turtle eggs comes from natural captivity in Lhok Pante Tibang Beach, Banda Aceh. The research parameters included the number of isolates, macroscopic morphology (shape, edge, elevation, and color of isolates), cell morphology (Gram staining and cell shape), and biochemical tests. Isolation method using spread plate and quadrant method. The isolation results obtained five isolates which had varying macroscopic morphology. The five isolates were Gram negative and formed such as trunk. Identification results found that there were three genus from Enterobacteriaceae videlicet two isolates were Enterobacter, two isolates as Shigella and the other were Klebsiella.   

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