11. The Isolation And Identification Of Bacteria Salmonella Sp On Quail Egg Shell In Traditional Markets Ulee Kareng Banda Aceh

This research aim was to determine the level of bacterial contamination of Salmonella sp on quail egg. The samples used in this research was 24 shell of quail eggs obtained from eight stores in traditional markets in Ulee Kareung Banda Aceh. The shell was crushed with a mortar and then cultured on SCB media (selenite cysteine broth). Isolation and identification was conducted by using SSA media culture, Gram staining, and biochemical tests. The results of this research showed that the shell of quail eggs contained Salmonella sp. Therefore it can be concluded that the quail eggs sold in Ulee Kareung traditional market were contaminated by Salmonella sp.

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Karakterisasi dan Identifikasi Bakteri Enterobacteriaceae pada Telur Penyu Lekang (Lepidochelys olivacea) asal Lhok Pante Tibang, Banda Aceh

Jurnal Sain Veteriner ◽

10.22146/jsv.48514 ◽

2019 ◽

Vol 37 (1) ◽

pp. 41

Author(s):

Risa Nursanty *, Widya Sari, Safranita Risa Nursanty *, Widya Sari, Safranita ◽

Widya Sari ◽

Safranita Safranita

Keyword(s):

Cell Shape ◽

Bacterial Contamination ◽

Isolation Method ◽

Contributing Factors ◽

Olive Ridley ◽

Biochemical Tests ◽

Lepidochelys Olivacea ◽

Gram Staining ◽

Olive Ridley Turtle ◽

Banda Aceh

The population of olive ridley turtle (Lepidochelys olivacea) are currently thretened with extinction, among the contributing factors is the failure of turtle eggs to hatch. The condition can be caused by the presence of bacterial contamination, especially group Enterobacteriaceae. The purpose of this research was to isolate the group of Enterobacteriaceae bacteria from olive ridley turtle. The sample used were shells from olive ridley turtle eggs comes from natural captivity in Lhok Pante Tibang Beach, Banda Aceh. The research parameters included the number of isolates, macroscopic morphology (shape, edge, elevation, and color of isolates), cell morphology (Gram staining and cell shape), and biochemical tests. Isolation method using spread plate and quadrant method. The isolation results obtained five isolates which had varying macroscopic morphology. The five isolates were Gram negative and formed such as trunk. Identification results found that there were three genus from Enterobacteriaceae videlicet two isolates were Enterobacter, two isolates as Shigella and the other were Klebsiella.

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Identification of Bacteria on Seblak Food around University of Jember Based on Microbiological Criteria of Processed Food

BIOEDUKASI ◽

10.19184/bioedu.v18i1.14835 ◽

2020 ◽

pp. 34

Author(s):

Rizka Maulidya Cahyani ◽

Joko Waluyo ◽

Mochammad Iqbal

Keyword(s):

Morphological Characteristics ◽

Hot Water ◽

Biochemical Tests ◽

Isolation And Identification ◽

Gram Staining ◽

Microbiological Criteria ◽

Identification Of Bacteria ◽

Oxidase Test ◽

Safe Food

The quality of food that is good in bacteriological, chemical and physical must always be maintained in order to avoid diseases or health problems. Healthy and safe food is an important factor to improve the standard of public health. Seblak is a ready-to-eat Indonesian food which until now has never been carried out research about what kinds of bacteria in it. Seblak is a food made from raw crackers which is then deliberately soaked using hot water to have a chewy texture. This study aims to determine what types of bacteria are contained in seblak, through the process of isolation and identification in the macroscopic, microscopic and biochemical way. This study used 5 samples, which was repeated 5 times for each sample. Bacteria were isolated from the sample using spread plate techniques and observed by growing colonies on the plate. Each different colonies was observed microscopically through gram staining and endospore staining. To strengthen the data, biochemical tests were also carried out, biochemical tests that have been done in this study were the oxidase test, catalase test, and indole test. The results of the study showed that the bacteria that were found from the samples are in the genus of Bacillus sp. because they show the morphological characteristics of the colonies that form concentric circles, meanwhile, microscopic observations show morphological characteristics of cells in the form of gram-negative bacilli and have the endospores.

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Isolation and Identification of Bacteria from the Workers of Live Bird Markets at Mymensingh, Bangladesh

Journal of Bio-Science ◽

10.3329/jbs.v17i0.7121 ◽

1970 ◽

Vol 17 ◽

pp. 135-138

Author(s):

S Sarker ◽

S Talukder ◽

EH Chowdhury ◽

PM Das

Keyword(s):

Escherichia Coli ◽

Dna Isolation ◽

Biochemical Tests ◽

Isolation And Identification ◽

E Coli ◽

Live Bird Markets ◽

Nasal Swabs ◽

Identification Of Bacteria ◽

Live Bird

Context: Identification of bacteria from the workers of live bird markets is important factor for zoonotic aspects and for implementing appropriate control strategies.Objectives: To determine the occurrence of bacteria especially Salmonella sp. and Escherichia coli from the workers of live bird markets.Materials and Methods: A total of 40 samples were collected from hand washes (n=20) and nasal swabs (n=20) of the associated workers in urban and suburban live bird markets. Bacteria were isolated in different media, and identification was performed based on the staining, cultural and some biochemical tests. For Salmonella sp., DNA was extracted using a DNA isolation kit and rfbs gene was amplified by using commercial PCR kit.Results: The bacteria such as Salmonella sp. and E. coli were detected in the samples by several microbial tests. The prevalence of Salmonella sp. was 40% and 30%, and E. coli was 70% and 40% in the hand washes and nasal swabs respectively of the workers of urban and periurban live bird markets.Conclusion: The results obtained in this study suggest that the appropriate precautions should be taken during and subsequent to the handling of live birds to minimize the risk of zoonotic diseases.Key words: Salmonella; Escherichia coli; live bird markets; isolation and identificationDOI: 10.3329/jbs.v17i0.7121J. bio-sci. 17: 135-138, 2009

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Sustainable waste management distribution in traditional marketplace during pandemic COVID-19. Case study: Pasar Al-Mahirah, Banda Aceh city, Indonesia

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/881/1/012053 ◽

2021 ◽

Vol 881 (1) ◽

pp. 012053

Author(s):

E N Rauzi ◽

Z Sahputra ◽

F Y Pradika ◽

A Zahrah

Keyword(s):

Waste Management ◽

Sustainable Management ◽

Natural Ventilation ◽

Organic Waste ◽

Design Solution ◽

Traditional Markets ◽

Traditional Market ◽

Banda Aceh ◽

Sophisticated Technology

Abstract Waste management has consistently been challenged around the globe for decades, yet sophisticated technology has not managed the pile of trash, particularly in developing countries. One of the most often produced waste is the traditional market. The study aims to identify how the conventional market disposes of its regular waste and investigate the space quality as a crowded place during the pandemic of Covid-19. The research method is observing and analysing a case study located in Banda Aceh City, Indonesia. Pasar Al-Mahirah is one of the traditional markets which distributing its complete regular waste directly to landfills. The result indicates that waste management is not sustainable because it lacks sorting and processing before. In terms of C-19 spreading, the space inside the building is vulnerable even though it has excellent natural ventilation and sunlight. This study performed a visual design solution for distributing waste inside the building, sustainable management based on 3R principle methods, and composting through aerobic methods for organic waste. Moreover, the stalls arrangement and the circulation for users are purposed in a layout plan to prevent a higher risk of the rising of Coronavirus transmissions.

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Pathological study on the upper respiratory tract infection of chickens and isolation, identification of causal bacteria

Bangladesh Veterinarian ◽

10.3329/bvet.v28i2.10677 ◽

2012 ◽

Vol 28 (2) ◽

pp. 60-69 ◽

Cited By ~ 3

Author(s):

N. Popy ◽

M. Asaduzzaman ◽

M. S. Miah ◽

A. Siddika ◽

M. A. Sufian ◽

...

Keyword(s):

Plasma Cells ◽

Nasal Passage ◽

Upper Respiratory Tract ◽

Pathological Study ◽

Biochemical Tests ◽

Isolation And Identification ◽

Nasal Sinus ◽

Mucosal Layer ◽

Identification Of Bacteria ◽

Upper Respiratory

The proportional occurrence of bacteria and pathological lesions in the nasal sinuses and trachea of dead chickens were determined during 2008-2009. Nasal sinus and tracheal swabs from 50 dead birds were collected in sterile nutrient broth. The histopathological samples were collected in 10% neutral buffered formalin and studied with light microscope. The isolation and identification of bacteria were performed by culture, staining and biochemical tests. The proportional occurrence of bacteria in trachea (n = 50) and nasal sinuses (n = 50) of dead chickens was Klebsiella sp. (6.0%), Escherichia coli (38.8%), Pasteurella sp. (8.6%), Bacillus sp. (5.2%) and Staphylococcus sp. (41.4%). Congested trachea (n=3) and mucus-filled sinuses (n = 3) of dead chickens were studied for histopathology. Microscopically, rhinitis was characterized by infiltration of macrophages, lymphocytes and few neutrophils. The epithelium of nasal passage revealed pyknotic nucleus with disruption of epithelium. There was sinusitis with purulent and necrotic changes around the nasal sinus. The nasal sinuses were infiltrated with macrophages, lymphocytes and few plasma cells. The mucosal layer of the nasal turbinates showed pus and necrosis. There was disruption of different mucous glands with accumulation of macrophages, lymphocytes and plasma cells in the submucosa.DOI: http://dx.doi.org/10.3329/bvet.v28i2.10677 Bangl. vet. 2011. Vol. 28, No. 2, 60 69

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CEMARAN Staphylococcus aureus PADA DAGING SAPI YANG DIPASARKAN DI PASAR TRADISIONAL KOTA BANDA ACEH (Staphylococcus aureus Contamination in Beef from Traditional Markets in Banda Aceh)

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v9i2.3813 ◽

2015 ◽

Vol 9 (2) ◽

Author(s):

Jhon Leo Danubrata ◽

Fakhrurrazi Fakhrurrazi ◽

Maryulia Dewi ◽

Sulasmi Sulasmi ◽

Faisal Jamin ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Microbial Contamination ◽

National Standard ◽

Traditional Markets ◽

Traditional Market ◽

Colony Counter ◽

Banda Aceh

This research aimed to determine the total amount of Staphylococcus aureus (S. aureus) contamination in beef sold in traditional market in Banda Aceh. Total of 16 beef samples were used in this research, 2 samples from Beurawe market, 10 samples from Peunayong market, and 4 samples from Seutui market. Samples were cultured on manitol salt agar. The number of S. aureus colonies was calculated using colony counter, and the result was compared to the limit of microbial contamination based on Indonesian National Standard (SNI). The result of this research showed that the highest level of S. aureus contamination was derived from Beurawe market. It is concluded that all beef sold by seller from traditional market in Banda Aceh were contaminated by S. aureus.Key words: Staphylococcus aureus, meat, traditional market, number of contamination

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Bacteriological and Pathological Investigation of Nasal Passage Infections of Chickens (Gallus gallus)

The Agriculturists ◽

10.3329/agric.v11i1.15241 ◽

2013 ◽

Vol 11 (1) ◽

pp. 47-55 ◽

Cited By ~ 1

Author(s):

MS Hossain ◽

S Akter ◽

M Ali ◽

PM Das ◽

MM Hossain

Keyword(s):

Escherichia Coli ◽

Mixed Infection ◽

Nasal Passage ◽

Biochemical Tests ◽

Isolation And Identification ◽

Tissue Samples ◽

E Coli ◽

Identification Of Bacteria ◽

Nasal Passages ◽

Diagnostic Centre

An experiment was conducted at the Bangladesh Agricultural University, Mymensingh during July 2011 to May 2012 to investigate the prevalence of bacteria as well as pathological lesions in the nasal passages of dead chickens. Thirty nasal passage swabs from 30 dead birds (20 from SK Veterinary Diagnostic Centre (SKVDC) and 10 from the Department of Pathology, BAU were collected in sterile nutrient broth. The histopathological samples (n=6) were collected in 10% neutral buffered formalin. The isolation and identification of bacteria were performed by culturing in different media, staining and biochemical tests. The collected tissues were fixed, processed, sectioned, stained and studied with light microscope. The prevalence of bacteria 30.43% Escherichia coli, 47.83% Staphylococcus sp., 13.04% Pasteurella sp., and 8.69% Klebsiella sp. were found in nasal passages (n=30) of dead chickens. Two cases of mixed infection with E. coli and Pasteurella isolates, and one case with Klebsiella and E. coli isolates were identified. Six tissue samples of grossly identifiable lesions such as congested and mucus filled nasal passages from 6 dead chickens were processed for histopathology. Microscopically, the section of nasal passages in general showed congested mucosa with excessive infiltration of heterophils and lymphocytes. The lining epithelia of nasal passage revealed acanthosis, hyperkeratosis and disruption of nasal epithelia. There was also hyperplasia of different mucous glands of nasal passages. Klebsiella sp. affected nasal tissues showed comparatively severe lesions than that of other bacterial infection in chickens. DOI: http://dx.doi.org/10.3329/agric.v11i1.15241 The Agriculturists 2013; 11(1) 47-55

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Isolation and Identification of Bacteria from Chlorpyrifos Polluted Soil

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.45.23 ◽

2015 ◽

Vol 45 ◽

pp. 23-26

Author(s):

M. Santhoshkumar ◽

T. Mahakavi ◽

L. Baskaran

Keyword(s):

Contaminated Soils ◽

Plant Root ◽

Polluted Soil ◽

Biochemical Tests ◽

Isolation And Identification ◽

Physiochemical Properties ◽

Celosia Cristata ◽

Cultivated Plant ◽

Identification Of Bacteria ◽

And Control

The present study aimed to isolate and identify the chlorpyrifos resistant soil bacteria from contaminated soils in order to be used for bioremediation of polluted environments. Bacteria were isolated from two cultivated plant root rhizopheric soil of Cocks comb (Celosia cristata) and Marigold (Tagetes erecta).The pesticide was tested at 5 elevated doses,0.5%, 1%, 2%, 2.5% and control. Physiochemical properties of soils, PH, Electrical conductivity, Organic carbon, Organic matter, Nitrogen, phosphorus, potassium, Manganese and Iron were analyzed. Based on morphological and biochemical tests the isolates were identified as Bacillus subtilis, Pseudomonas putida, Pseudomonas aeruginosa. Thus, the organisms can be exploited for bioremediation of chlorpyrifos polluted soil, and their ability to degrade other organophosphates pesticide.

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ISOLASI DAN IDENTIFIKASI BAKTERI AEROB YANG BERPOTENSI MENYEBABKAN INFEKSI NOSOKOMIAL DI RUANG RAWAT INAP MATA IRINA F RSUP PROF. DR. R.D. KANDOU MANADO

Jurnal e-Biomedik ◽

10.35790/ebm.4.1.2016.11041 ◽

2016 ◽

Vol 4 (1) ◽

Author(s):

Ameliya S. Japanto ◽

Standy Soeliongan ◽

Fredine E. S. Rares

Keyword(s):

Bacillus Subtilis ◽

Nosocomial Infections ◽

Agar Medium ◽

Enterobacter Cloacae ◽

Hospital Environment ◽

Aerobic Bacteria ◽

Enterobacter Agglomerans ◽

Biochemical Tests ◽

Gram Staining ◽

Identification Of Bacteria

Abstract: Nosocomial infections are infections acquired or occurring while patients were hospitalized. Nosocomial infections can be caused by various agents of disease, like bacteria. The bacteria are found in the hospital environment, including the inpatient unit. Objective: to know the pattern of aerobic bacteria that could potentially cause nosocomial infections in inpatient eyes unit IRINA F Prof. Dr. R. D. Kandou Manado. Methods: 14 swabs were taken at the surface of patient’s beds, treatment rooms and 8 samples of air space. Identification of bacteria carried on an agar medium isolation, Gram staining and biochemical tests. Results: The obtained bacteria identified six types of bacteria, such as Bacillus subtilis, Lactobacillus sp., Coccus negative Gram, Enterobacter agglomerans, Enterobacter cloacae, and Staphylocccus epidermidis. Conclusion: Bacillus subtilis is the bacteria that most commonly found in inpatient eyes unit IRINA FKeywords: nosocomial infections, patterns of aerobic bacteria, inpatient eye unitAbstrak: Infeksi nosokomial adalah infeksi yang didapat atau terjadi saat pasien dirawat di rumah sakit. Infeksi nosokomial dapat disebabkan oleh berbagai agen penyakit, salah satunya ialah bakteri. Bakteri penyebab infeksi sering ditemukan di lingkungan rumah sakit, termasuk di ruang rawat inap. Tujuan : mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di ruang rawat inap mata IRINA F RSUP Prof. Dr. R. D. Kandou Manado. Metode: sampel diambil pada 14 usapan perabotan ruangan, ruangan perawatan dan 8 sampel udara ruang. Identifikasi bakteri dilakukan isolasi pada media agar, pewarnaan Gram dan uji biokimia. Hasil: bakteri yang teridentifikasi didapatkan enam jenis bakteri, yaitu Bacillus subtilis, Lactobacillus sp., Coccus Gram negatif, Enterobacter agglomerans, Enterobacter cloacae, dan Staphylocccus epidermidis. Kesimpulan: Bakteri Bacillus subtilis merupakan bakteri yang paling banyak ditemukan di ruang rawat inap mata IRINA FKata kunci: infeksi nosokomial, pola bakteri aerob, ruang rawat inap mata

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MODEL OPTIMALISASI FUNGSI PASAR TRADISIONAL DI KOTA BANDA ACEH (Studi Kasus Pasar Newtown Lamdom)

Teras Jurnal ◽

10.29103/tj.v11i2.521 ◽

2021 ◽

Vol 11 (2) ◽

pp. 463

Author(s):

Ibnu Sa'dan Sa'dan ◽

N Fadhli ◽

Ashfa Ashfa

Keyword(s):

Focus Group ◽

Focus Group Discussion ◽

Group Discussion ◽

Regression Method ◽

City Government ◽

Raw Material ◽

Traditional Markets ◽

Traditional Market ◽

The City ◽

Banda Aceh

Abstrak Pasar tradisional adalah salah satu aspek yang dapat menunjang pertumbuhan ekonomi di Kota Banda Aceh. Pasar Tradisional Newtown Lamdom (PTNL) merupakan salah satu pasar tradisional di Kota Banda Aceh yang belum berfungsi sebagaimana mestinya, tidak ada satupun pedagang yang berjualan di sana. Penelitian ini bertujuan untuk mengetahui faktor-faktor apa saja yang memiliki pengaruh terhadap tidak optimalnya fungsi PTNL dan merumuskan strategi dalam meningkatkan pengelolaan PTNL. Strategi dalam mengoptimalkan fungsi pasar tradisional sudah diidentifikasi dengan menyebarkan kuesioner melalui observasi lapangan dan melalui google form, data kuesioner diolah menggunakan metode analisis regresi ordinal, kemudian akan diperoleh hasil penyebab tidak optimalnya fungsi pasar tradisional Newtown Lamdom selanjutnya, faktor-faktor tersebut dianalisis dengan menggunakan metode Focus Group Discussion (FGD). hasil dari serangkaian analisis yang dilakukan menunjukkan bahwa lokasi PTNL memiliki pengaruh 82,1%, sarana PTNL memiliki pengaruh sebesar 74,7% dan prasarana PTNL memiliki pengaruh sebesar 22,7% terhadap factor yang mempengaruhi tidak optimalnya fungsi PTNL, untuk dapat mengoptimalkan fungsi PTNL perlu menyiapkan lokasi dan sarana sesuai dengan kebutuhan pasar seperti ketersedian lahan parkir, tersedianya transportasi umum menuju pasar, air bersih, listrik dan ketersediaan bahan baku, selain itu juga perlu ditinjau aspek kebijakan pemerintah seperti melakukan sosialisasi kepada pedagang dan pembeli untuk dapat mengoptimalkan fungsi PTNL. Kata Kunci: Strategi, optimalisasi pasar, pasar tradisional, fungsi pasar Abstract Traditional market is one of the supporting aspects for economic growth in Banda Aceh. Newtown Lamdom Market (PTNL) is one of traditional markets in Banda Aceh that has not been functioning properly from year to year as there is no single trader who sells there. This study aims to determine the factors causing the function of the traditional market is not optimal and to formulate the strategy in order to improve the management of Newtown Lamdom Traditional Market. The author identified the strategy in optimizing the function of traditional market by conducting field observation. The results of observation are then analyzed by using the Ordinal Regression Method to determine the factors causing the function of the traditional market are not optimal. Afterwards, those factors are analyzed through Focus Group Discussion (FGD) with the determined informants. The results of series of analyses conducted show that the City Government of Banda Aceh is required to rehabilitate the Newtown Lamdom market buildings, equip the market facilities and infrastructure, provide raw material needs, and conduct socialization to traders and buyers in order to optimize the function of Newtown Lamdom Market. Keywords: Strategy, Market Optimization, Traditional Market, market function

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