Isolation and Identification of Genus Salmonella and Shigella from Sumatran Orangutan (Pongo abelii) Feces in Orangutan Reintroduction Center, Jantho

This study was done to identified bacteria of genus Salmonella and Shigella from sumatran orangutan (Pongo abelii) feces. Nine semi wild orangutan fecal samples were obtained in Orangutan Reintroduction Center, Cagar Alam Pinus Jantho, Aceh Besar. The samples were processed using Carter methode. The selective media used were Salmonella Shigella Agar (SSA), Eosin Methylen Blue (EMB), and MacConkey Agar. The separated colonies which grawn in SSA media were observed for their morphology, and followed by Gram staining methode then continued with biochemical test such as Indol, Methyl Red (MR), Voges Proskauer (VP), Sulfid Indol Motility (SIM), Simmons Citrate (SC), and sugar test such as glucose, sucrose, lactose, and manitol. From nine samples, one orangutan was infected with Salmonella and another one was infected with Shigella. All of the samples were identified with Escherichia. In Conclusion, orangutan lived at Reintroduction Center, Jantho infected with Salmonella and Shigella bacteria.Key words: feces, orangutan, Salmonella, Shigella

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9. The Isolation of Steptococcus sp on horse’s dental caries in Aceh Tengah Regency

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v13i1.4218 ◽

2019 ◽

Vol 13 (1) ◽

pp. 65-71

Author(s):

Sagasias Ahmad ◽

Erina Erina ◽

M Daud AK ◽

Darmawi Darmawi ◽

Fakhrurrazi Fakhrurrazi ◽

...

Keyword(s):

Dental Caries ◽

Nutrient Broth ◽

Biochemical Test ◽

Selective Media ◽

Bacteria Identification ◽

Gram Staining

The purpose of this study was to detect the existence of the Streptococcus sp bacteria in the horses’ dental caries in Aceh Tengah Regency. The caries samples used in this study were taken from 8 horses in Aceh Tengah Regency. The samples were collected using swab on the teeth and then put into Nutrient Broth (NB) media and further were cultured on selective media TSY20B. The bacteria identification was conducted by observing the bacteria’s morphology, Gram staining and biochemical test. The eight samples of the horses’ dental caries showed that Streptococcus sp bacteria could be isolated from all of the collected samples. From this research it could be concluded that Streptococcus sp bacteria was found in the horses’ dental caries and it was one of the causes of the dental caries.

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ISOLASI DAN IDENTIFIKASI BAKTERI ASAM LAKTAT (BAL) DARI BUAH MANGGA (Mangifera indica L.)

Jurnal Ilmiah Manuntung ◽

10.51352/jim.v1i2.29 ◽

2017 ◽

Vol 1 (2) ◽

pp. 159

Author(s):

Arsyik Ibrahim ◽

Aditya Fridayanti ◽

Fila Delvia

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Smooth Surface ◽

Mangifera Indica ◽

Isolation And Identification ◽

Selective Media ◽

Gram Staining ◽

Surface Curve ◽

Mrs Broth

The research has been done for the isolation and identification of lactic acid bacteria (LAB) from mango (Mangifera indica L.). This research aimed to isolated of lactic acid bacteria that is in mango (Mangifera indica L.) and determine the characteristics of lactic acid bacteria isolate (LAB) of mango (Mangifera indica L.). The method used is spoiled technique of mango (Mangifera indica L.) and isolation using selective media MRS Broth and MRS Agar. The identification isolate of lactic acid bacteria (LAB) used methods macroscopically and microscopically with indirect coloring, gram staining and used biochemical with katalase testing. The results obtained in the form of characteristic isolate of lactic acid bacteria displayed form of bacteria with circle, smooth surface, curve, entire side and white. The microscopically displayed stick form of bacteria and purple with gram coloring

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10. Isolation and Identification of Lactic Acid Bacteria Genus Pediococcus from Sumatran Orangutan (Pongo abelii) faeces at Kandi Zoo and Kinantan Zoo West Sumatera

Jurnal Medika Veterinaria ◽

10.21157/j.med.vet..v11i1.3524 ◽

2017 ◽

Vol 11 (1) ◽

pp. 59-65

Author(s):

Resti Reimena ◽

Erina Erina ◽

Darniati Darniati ◽

Fakhrurrazi Fakhrurrazi ◽

Darmawi Darmawi ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Cell Structure ◽

Isolation And Identification ◽

Plate Method ◽

Pongo Abelii ◽

Sumatran Orangutan ◽

Oxidase Test ◽

Characteristic Features ◽

De Man

This research has purposes to isolating and identifying the lactic acid bacteria genus Pediococcus in sumatran orangutan (Pongo abelii) feaces. Feaces are isolated from one orangutan at Kandi Zoo and two orangutans at Kinantan Zoo. The isolating of the lactic acid bacteria was done by using streak plate method with the MRSA (de Man Rogosa Sharpe Agar) specific media. Identification was done by the morphological observing, Gram stain, catalase test, oxidase test and biochemical test. The result showed that one isolate feaces from Kandi Zoo has cocci shaped with the isolate prediction approaches genus Pediococcus sp. The characteristic features of isolate are cocci shaped, Gram positive, tetrad cell structure, nonmotile, catalase negative, and oxidase negative. Based on these characteristics, it can be concluded that there is a lactic acid bacteria coloni which grouped into genus Pediococcus in male orangutan feaces from Kandi Zoo

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Isolation and molecular characterization of Arcobacter butzleri and Arcobacter cryaerophilus from the pork production chain in Brazil

Pesquisa Veterinária Brasileira ◽

10.1590/1678-5150-pvb-4709 ◽

2018 ◽

Vol 38 (3) ◽

pp. 393-399 ◽

Cited By ~ 4

Author(s):

Débora D.S. Gobbi ◽

Maria G. Spindola ◽

Luisa Z. Moreno ◽

Carlos E.C. Matajira ◽

Maria G.X. Oliveira ◽

...

Keyword(s):

Animal Origin ◽

Isolation And Identification ◽

Production Chain ◽

Fecal Samples ◽

Pork Production ◽

True Incidence ◽

Arcobacter Butzleri ◽

Meat Markets ◽

Human Infections

ABSTRACT: Arcobacter is an emerging zoonotic pathogen, and the major transmission routes to humans are the handling or consumption of contaminated raw/undercooked food products of animal origin, water and seafood. The isolation and identification of Arcobacter species are not routine in clinical laboratories; therefore, its true incidence in human infections may be underestimated. The present study aimed to isolate and characterize Arcobacter from carcasses and fecal samples collected at swine slaughterhouses and from meat markets in São Paulo State, Brazil. The isolates were identified using multiplex-PCR to differentiate the species and analyzed by single-enzyme amplified fragment length polymorphism (SE-AFLP). Arcobacter spp. were isolated from 73.0% of swine carcasses, 4% of fecal samples and 10% of pork samples. A. butzleri was the most prevalent species identified, followed by A. cryaerophilus. Interestingly, the carcasses presented higher frequency of A. butzleri isolation, whereas only A. cryaerophilus was isolated from fecal samples. SE-AFLP enabled the characterization of A. butzleri and A. cryaerophilus into 51 and 63 profiles, respectively. The great genetic heterogeneity observed for both species corroborates previous reports. This study confirms the necessity for a standard isolation protocol and the improvement of molecular tools to further elucidate Arcobacter epidemiology.

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Molecular Diversity of Methanogens in Fecal Samples From Captive Sumatran Orangutans (Pongo abelii)

American Journal of Primatology ◽

10.1002/ajp.21992 ◽

2012 ◽

Vol 74 (5) ◽

pp. 408-413 ◽

Cited By ~ 13

Author(s):

HANNAH V. FACEY ◽

KORINNE S. NORTHWOOD ◽

ANDRÉ-DENIS G. WRIGHT

Keyword(s):

Molecular Diversity ◽

Fecal Samples ◽

Pongo Abelii

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Enumeration and Identification of Coliform Bacteria Injured by Chlorine or Fungicide Mixed with Agricultural Water

Journal of Food Protection ◽

10.4315/0362-028x.jfp-16-124 ◽

2016 ◽

Vol 79 (10) ◽

pp. 1789-1793 ◽

Cited By ~ 8

Author(s):

HIDEMI IZUMI ◽

YUJI NAKATA ◽

AYANO INOUE

Keyword(s):

Cell Injury ◽

Pond Water ◽

Coliform Bacteria ◽

Agricultural Water ◽

Isolation And Identification ◽

Electrolyzed Water ◽

Selective Media ◽

E Coli ◽

Pure Cultures ◽

Water Pond

ABSTRACT Chemical sanitizers may induce no injury (bacteria survive), sublethal injury (bacteria are injured), or lethal injury (bacteria die). The proportion of coliform bacteria that were injured sublethally by chlorine and fungicide mixed with agricultural water (pond water), which was used to dilute the pesticide solution, was evaluated using the thin agar layer (TAL) method. In pure cultures of Enterobacter cloacae, Escherichia coli, and E. coli O157:H7 (representing a human pathogen), the percentage of chlorine-injured cells was 69 to 77% for dilute electrolyzed water containing an available chlorine level of 2 ppm. When agricultural water was mixed with electrolyzed water, the percentage of injured coliforms in agricultural water was 75%. The isolation and identification of bacteria on TAL and selective media suggested that the chlorine stress caused injury to Enterobacter kobei. Of the four fungicide products tested, diluted to their recommended concentrations, Topsin-M, Sumilex, and Oxirane caused injury to coliform bacteria in pure cultures and in agricultural water following their mixture with each pesticide, whereas Streptomycin did not induce any injury to the bacteria. The percentage of injury was 45 to 97% for Topsin-M, 80 to 87% for Sumilex, and 50 to 97% for Oxirane. A comparison of the coliforms isolated from the pesticide solutions and then grown on either TAL or selective media indicated the possibility of fungicide-injured Rahnella aquatilis, Yersinia mollaretii, and E. coli. These results suggest the importance of selecting a suitable sanitizer and the necessity of adjusting the sanitizer concentration to a level that will kill the coliforms rather than cause sanitizer-induced cell injury that can result in the recovery of the coliforms.

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PATHOGENIC BACTERIA ISOLATED FROM COCKROACHES FOUND IN FOOD PREMISES

Jurnal Teknologi ◽

10.11113/jt.v78.9057 ◽

2016 ◽

Vol 78 (6-8) ◽

Author(s):

Anis Husna Wahab ◽

Mohd Pozi Mohd Tahir ◽

Emida Mohamed

Keyword(s):

Pathogenic Bacteria ◽

Safety Issue ◽

Internal Surface ◽

Nutrient Agar ◽

Pathogenic Microorganisms ◽

Macconkey Agar ◽

Selective Media ◽

Blatta Orientalis ◽

Shigella Species ◽

Internal Body

Cockroaches play an important role in transmission of different diseases either mechanically and biological because they spread and harbor various species of microorganism. This study was carried out to isolate the pathogenic bacteria from external and internal body of cockroaches at food premises. A total of 48 cockroaches, 32 of Periplanetta americana and 16 of Blatta orientalis, were collected at food premises in Shah Alam, Selangor, Malaysia. Medically important bacteria were isolated from external and internal surface by using microbial technique. From the collected Periplanetta americana and Blatta orientalis, E.coli,Salmonella species, Shigella species, and Staphylococcus were isolated and identified in their selective media such as nutrient agar, MacConkey agar, and Xylone Lysine Deoxycholate (XLD) agar using microbial technique. The data obtained from the study emphasized that cockroaches may play an important role in the transmission of pathogenic bacteria to human. The presence of cockroaches at food premises maybe due to unhygienic environment. Thus it may pose as a safety issue as they may carries of pathogenic microorganisms that can threaten the health of individuals.

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BIODEGRADASI LIMBAH KULIT BUAH KAKAO (Theobroma cacao L.) OLEH KAPANG Aspergillus niger DENGAN VARIASI JUMLAH INOKULUM DAN WAKTU INKUBASI

SIMBIOSIS Journal of Biological Sciences ◽

10.24843/jsimbiosis.2020.v08.i01.p06 ◽

2020 ◽

Vol 8 (1) ◽

pp. 41

Author(s):

Alfian Siswanto ◽

Ida Bagus Gede Darmayasa ◽

I Ketut Muksin

Keyword(s):

Aspergillus Niger ◽

Incubation Time ◽

Theobroma Cacao ◽

In Vitro Test ◽

Isolation And Identification ◽

Inoculum Concentration ◽

Selective Media ◽

Theobroma Cacao L

Crop waste or cacao (Theobroma cacao L.) processing waste that doesn’t handle with care can polluting the environment around the farm. Rind of cacao can be used for composting, but it can be less effective because the nutrient of fresh cacao rind too complex and the ratio of C/N too high. This research intention to knows the potential of Aspergillus niger to degrade cellulose on waste cacao rind who have high ratio of C/N, with combination inoculum concentration and incubation time. The methods of this research begin with isolation and identification of A. niger from litter of cacao field, then the in vitro test done by CMCA (Carboxyl Methyl Cellulose Agar) selective media, and then the cacao rind was testing by in vivo method. The results of this research shown that A. niger can degrade cellulose by in vitro ways, it can be seen by the clear zone that formed on CMCA selective media. The in vivo test on cacao rind shown decreasing of C/N ratio between 18,940%-40,623%. Combination of A3B3 treatment (15 mL inoculum concentration, 28 days incubation time) indicate the lowest C/N ratio 18,940%.

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