scholarly journals CRISPR/Cas9-mediated targeted mutagenesis of GmLHY genes alters plant height and internode length in soybean

2019 ◽  
Author(s):  
Qun Cheng ◽  
Lidong Dong ◽  
Tong Su ◽  
Tingyu Li ◽  
Zhuoran Gan ◽  
...  
Keyword(s):  
Plant Height ◽  
Regulatory Networks ◽  
Molecular Mechanisms ◽  
Targeted Mutagenesis ◽  
Myb Transcription Factor ◽  
Soybean Plant ◽  
Transgenic Soybean ◽  
In The Wild ◽  
Quadruple Mutant ◽  
Soybean Plants

Abstract Background: Soybean (Glycine max) is an economically important oil and protein crop. Plant height is a key trait that significantly impacts the yield of soybean; however, research on the molecular mechanisms associated with soybean plant height is lacking. The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (CRISPR-associated system 9) system is a recently developed technology for gene editing that has been utilized to edit the genomes of crop plants. Results: Here, we designed four gRNAs to mutate four LATE ELONGATED HYPOCOTYL (LHY) genes in soybean. In order to test whether the gRNAs could perform properly in transgenic soybean plants, we first tested the CRISPR construct in transgenic soybean hairy roots using Agrobacterium rhizogenes strain K599. Once confirmed, we performed stable soybean transformation and obtained 19 independent transgenic soybean plants. Subsequently, we obtained one T1 transgene-free homozygous quadruple mutant of GmLHY by self-crossing. The phenotypes of the T2-generation transgene-free quadruple mutant plants were observed, and the results showed that the quadruple mutant of GmLHY displayed reduced plant height and shortened internodes. The levels of endogenous gibberellic acid (GA3) in Gmlhy1a1b2a2b was lower than in the wild type (WT), and the shortened internode phenotype could be rescued by treatment with exogenous GA3. In addition, the relative expression levels of GA metabolic pathway genes in the quadruple mutant of GmLHY were significantly decreased in comparison to the WT. These results suggest that GmLHY encodes an MYB transcription factor that affects plant height through mediating the GA pathway in soybean. We also developed genetic markers for identifying mutants for application in breeding studies. Conclusions: Our results indicate that CRISPR/Cas9-mediated targeted mutagenesis of four GmLHY genes reduces soybean plant height and shortens internodes from 20 to 35 days after emergence (DAE). These findings provide insight into the mechanisms underlying plant height regulatory networks in soybean.

scholarly journals CRISPR/Cas9-mediated targeted mutagenesis of GmLHY genes alters plant height and internode length in soybean

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Qun Cheng ◽  
Lidong Dong ◽  
Tong Su ◽  
Tingyu Li ◽  
Zhuoran Gan ◽  
...  
Keyword(s):  
Plant Height ◽  
Regulatory Networks ◽  
Molecular Mechanisms ◽  
Targeted Mutagenesis ◽  
Myb Transcription Factor ◽  
Soybean Plant ◽  
Transgenic Soybean ◽  
In The Wild ◽  
Quadruple Mutant ◽  
Soybean Plants

Abstract Background Soybean (Glycine max) is an economically important oil and protein crop. Plant height is a key trait that significantly impacts the yield of soybean; however, research on the molecular mechanisms associated with soybean plant height is lacking. The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (CRISPR-associated system 9) system is a recently developed technology for gene editing that has been utilized to edit the genomes of crop plants. Results Here, we designed four gRNAs to mutate four LATE ELONGATED HYPOCOTYL(LHY) genes in soybean. In order to test whether the gRNAs could perform properly in transgenic soybean plants, we first tested the CRISPR construct in transgenic soybean hairy roots using Agrobacterium rhizogenes strain K599. Once confirmed, we performed stable soybean transformation and obtained 19 independent transgenic soybean plants. Subsequently, we obtained one T1 transgene-free homozygous quadruple mutant of GmLHY by self-crossing. The phenotypes of the T2-generation transgene-free quadruple mutant plants were observed, and the results showed that the quadruple mutant of GmLHY displayed reduced plant height and shortened internodes. The levels of endogenous gibberellic acid (GA3) in Gmlhy1a1b2a2b was lower than in the wild type (WT), and the shortened internode phenotype could be rescued by treatment with exogenous GA3. In addition, the relative expression levels of GA metabolic pathway genes in the quadruple mutant of GmLHY were significantly decreased in comparison to the WT. These results suggest that GmLHY encodes an MYB transcription factor that affects plant height through mediating the GA pathway in soybean. We also developed genetic markers for identifying mutants for application in breeding studies. Conclusions Our results indicate that CRISPR/Cas9-mediated targeted mutagenesis of four GmLHY genes reduces soybean plant height and shortens internodes from 20 to 35 days after emergence (DAE). These findings provide insight into the mechanisms underlying plant height regulatory networks in soybean.

scholarly journals CRISPR/Cas9-mediated targeted mutagenesis of GmLHY genes alters plant height and internode length in soybean

2019 ◽  
Author(s):  
Fanjiang Kong ◽  
Qun Cheng ◽  
Lidong Dong ◽  
Tong Su ◽  
Zhuoran Gan ◽  
...  
Keyword(s):  
Plant Height ◽  
Molecular Mechanisms ◽  
Internode Length ◽  
Targeted Mutagenesis ◽  
Myb Transcription Factor ◽  
Soybean Plant ◽  
Transgenic Soybean ◽  
Quadruple Mutant ◽  
Short Palindromic Repeat ◽  
Soybean Plants

Abstract Background: Soybean (Glycine max) is an economically important oil and protein crop. Plant height is a key trait that significantly impacts the yield of soybean; however, research on the molecular mechanisms associated with soybean plant height is lacking. The CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (CRISPR-associated) system is a recently developed technology for gene editing that has been utilized to edit the genomes of crop plants. Results: Here, we designed four gRNAs to mutate four LATE ELONGATED HYPOCOTYL ( LHY ) / CIRCADIAN CLOCK ASSOCIATED1 ( CCA1 )- LIKE ( LCL ) genes in soybean. In order to test whether the gRNAs could perform properly in transgenic soybean plants, we first tested the CRISPR construct in transgenic soybean hairy roots using Agrobacterium rhizogenesis strain K599. Once confirmed, we performed stable soybean transformation and obtained nineteen independent transgenic soybean plants. Subsequently, we obtained one T 1 transgene-free homozygous quadruple mutant of GmLCL by self-crossed. The phenotype of T 2 -generation transgene-free quadruple mutant plants were observed and the results showed that quadruple mutant of GmLCL displayed reduced plant height and shortened internodes. In addition, the relative expression levels of gibberellic acid (GA) metabolic pathway genes in the quadruple mutant of GmLCL were significantly decreased than wild type (WT). It suggests that GmLCLs encoding MYB transcription factor affect plant height through mediating the GA pathway in soybean. We also develop some genetic markers to identify mutant for assisting breeding studies. Conclusions: Our results indicate that CRISPR/Cas9-mediated targeted mutagenesis of four GmLCL genes reduce soybean plant height and shorten internodes. These findings suggest that manipulation of four GmLCL genes may improve yield by altered plant height and internode length in soybean.

scholarly journals CRISPR/Cas9-mediated targeted mutagenesis of GmLCL genes alters plant height and internode length in soybean

2019 ◽  
Author(s):  
Fanjiang Kong ◽  
Qun Cheng ◽  
Lidong Dong ◽  
Tong Su ◽  
Zhuoran Gan ◽  
...  
Keyword(s):  
Plant Height ◽  
New Technology ◽  
Internode Length ◽  
Targeted Mutagenesis ◽  
Myb Transcription Factor ◽  
Soybean Plant ◽  
Transgenic Soybean ◽  
Plant Oil ◽  
Quadruple Mutant ◽  
Soybean Plants

Abstract Background: Soybean ( Glycine max ) is an important economically crops for plant oil and protein in the world. The plant height as a key trait has significant effects on yield of soybean , however, the research on molecular mechanism for soybean plant height is still unclear. Recently, CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 (CRISPR-associated) system as a new technology for gene editing, has been rapidly utilized to edit the genomes of crop plants. Results: Here, we designed four gRNAs to mutate four LATE ELONGATED HYPOCOTYL ( LHY ) / CIRCADIAN CLOCK ASSOCIATED1 ( CCA1 )- LIKE ( LCL ) genes in soybean. In order to test whether the gRNAs could perform properly in transgenic soybean plants, we first tested the CRISPR construct in transgenic soybean hairy roots using Agrobacterium rhizogenesis strain K599. Once confirmed, we performed stable soybean transformation and obtained nineteen independent transgenic soybean plants. Subsequently, we obtained one T 1 transgene-free homozygous quadruple mutant of GmLCL by self-crossed. The phenotype of T 2 -generation transgene-free quadruple mutant plants were observed and the results showed that quadruple mutant of GmLCL displayed reduced plant height and shortened internodes. In addition, the relative expression levels of gibberellic acid (GA) metabolic pathway genes in the quadruple mutant of GmLCL were significantly decreased than wild type (WT). It suggests that GmLCLs encoding MYB transcription factor affect plant height through mediating the GA pathway in soybean. We also develop some genetic markers to identify mutant for assisting breeding studies. Conclusions: Our results indicate that CRISPR/Cas9-mediated targeted mutagenesis of four GmLCL genes reduce soybean plant height and shorten internodes. These findings suggest that manipulation of four GmLCL genes may improve yield by altered plant height and internode length in soybean.

Soybean Sensitivity to Florpyrauxifen-benzyl during Reproductive Growth and the Impact on Subsequent Progeny

2017 ◽  
Vol 32 (2) ◽  
pp. 135-140 ◽  
Author(s):  
M. Ryan Miller ◽  
Jason K. Norsworthy
Keyword(s):  
Plant Height ◽  
Field Studies ◽  
Yield Reduction ◽  
Growth Stages ◽  
Soybean Plant ◽  
Reproductive Growth ◽  
Similar Reduction ◽  
Structural Class ◽  
Soybean Plants ◽  
The Impact

AbstractTo address recent concerns related to auxin herbicide drift onto soybean, a study was developed to understand the susceptibility of the reproductive stage of soybean to a new auxin herbicide compared with dicamba. Florpyrauxifen-benzyl is under development as the second herbicide in a new structural class of synthetic auxins, the arylpicolinates. Field studies were conducted to (1) evaluate and compare reproductive soybean injury and yield following applications of florpyrauxifen-benzyl or dicamba across various concentrations and reproductive growth stages and (2) determine whether low-rate applications of florpyrauxifen-benzyl or dicamba to soybean in reproductive stages would have similar effect on the progeny of the affected plants. Soybean were treated with 0, 1/20, or 1/160, of the 1X rate of florpyrauxifen-benzyl (30 g ai ha−1) or dicamba (560 g ae ha−1) at R1, R2, R3, R4, or R5 growth stage. Soybean plant height and yield was reduced from 1/20X dicamba across all reproductive stages. High drift rates (1/20X) of florpyrauxifen-benzyl also reduced soybean plant height >25% and yield across R1 to R4 stages. Germination, stand, plant height, and yield of the offspring of soybean plants treated with dicamba and florpyrauxifen-benzyl were significantly affected. Dicamba applied at a rate of 1/20X at R4 and R5 resulted in 20% and 35% yield reduction for the offspring, respectively. A similar reduction occurred from florpyrauxifen-benzyl applied at R4 and R5 at the 1/20X rate, resulting in 15% to 24% yield reduction for the offspring, respectively. Based on these findings, it is suggested that growers use caution when applying these herbicides in the vicinity of reproductive soybean.

scholarly journals PENGARUH KOMPOSISI BIOFERTILIZER CAIR TERHADAP PERTUMBUHAN TANAMAN KEDELAI DI LAHAN GAMBUT (The Effect of Liquid Biofertilizer Composition on Soybean Plant Growth in Peat Land)

AgriPeat ◽  
2019 ◽  
Vol 19 (01) ◽  
pp. 30-36
Author(s):  
Administrator Journal
Keyword(s):  
Plant Height ◽  
Peat Soil ◽  
Marginal Land ◽  
Soybean Plant ◽  
Randomized Design ◽  
Soil Media ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Soybean Plants ◽  
Number Of Branches

ABSTRACThis study aims to find the most potent combination of local microorganisms as a liquidbiofertilizer on marginal land, such as peatland. The study used Completely Randomized Design onpeat soil media in polybags, with 8 (eight) treatments and 4 (four) replicates, namely liquidbiofertilizer composition comprising a group of local microorganisms: 1) KHY, 2) IBT, 3) KHY +IBT, 4) KHY + IGT, 5) IBT + IGT, 6) KHY + IBT + IGT, 7) EM4 (Control +), 8) Aquadest(Controls -). The variables observed were plant height, number of leaves, number of branches,number of flowers of soybean crop. The results showed that the composition of the liquidbiofertilizer EM4 gave the best results on the parameters of plant height, the number of leaves, thenumber of branches, and the amount of flowers on soybean plants tested in peat soil media. Thecombined composition of local microorganisms KHY + IBT + IGT containing bacterial populationsof phosphate solvent, nitrogen-fixing bacteria, and cellulitic fungi, are still in the same group asEM4 in the treatment of the number of leaves and the number of plant flowers, therefore thecomposition of these potential local microorganisms is developed to become a liquid biofertilizerKeywords: Liquid Biofertilizer, Soybean Growth, Peat Land

scholarly journals Development of the nodulated soybean plant after flooding of the root system with different sources of nitrogen

2005 ◽  
Vol 17 (3) ◽  
pp. 291-297 ◽  
Author(s):  
André Luís Thomas ◽  
Ladaslav Sodek
Keyword(s):  
Root System ◽  
Leaf Area ◽  
Plant Height ◽  
N2 Fixation ◽  
Dry Matter ◽  
Growth Parameters ◽  
Soybean Plant ◽  
Total N ◽  
Beneficial Effects ◽  
Soybean Plants

Flooding leads to hypoxia, a stress to which symbiotic N2 fixation is especially sensitive. The response of fully nodulated soybean plants to a 21-day period of flooding was studied by measurements of growth parameters and xylem transport of organic nitrogenous components to the shoot, in the presence and absence of NO3- and NH4+ in the medium. Flooding was found to seriously impair N2 fixation, irrespective of the N source, as indicated by strongly reduced xylem ureide levels. In the absence of a source of N, growth was strongly reduced during flooding while accumulation of N in the shoot was virtually abolished. Flooding in the presence of 5 mM NO3- or NH4+ led to the accumulation of total N in the shoot but only NO3- promoted increases in total dry matter, plant height and leaf area above that found in the absence of N. The accumulation of N, however, was lower than that of the non-flooded control for both NO3- and NH4+. The increases in total dry matter, plant height and leaf area with NO3- was as high as those of the non-flooded control. These data clearly show the beneficial effects of NO3- during a prolonged period of flooding of the nodulated root system of soybean.

scholarly journals The Growth and Production Improvement of Soybean Plant (Glycine max L.) by Applying The Local Microorganisms of Fruit Waste in Palopo

2020 ◽  
Vol 5 (1) ◽  
pp. 22-26
Author(s):  
Rahman Hairuddin
Keyword(s):  
Plant Height ◽  
Block Design ◽  
Experimental Unit ◽  
Soybean Plant ◽  
Flowering Period ◽  
Production Improvement ◽  
Randomized Block Design ◽  
Fruit Waste ◽  
Glycine Max L ◽  
Soybean Plants

This study was aimed to find out the effective local microorganism of fruit wastes concentration on the growth and production of the soybean plants. This study was conducted in the trial land of Campus 2, Faculty of Agriculture, Cokroaminoto University, Palopo from September to December 2019. The method used in this study was the randomized block design with 5 treatments and 4 replications, therefore there were 20 experimental units. Each experimental unit comprised 2 plant units, therefore there were 40 plant samples on the given concentration of P0: Control, P1: 50 mL/L fruit waste local microorganism concentration, P2: 100 mL/L fruit waste local microorganism concentration, P3: 150 mL/L fruit waste local microorganism concentration, and P4: 200 mL/L fruit waste local microorganism concentration. The results showed that the local microorganism application on the soybean plants had no significant effects on the plant height, total of leaves, flowering period, and a total of pods. The local fruit waste microorganism with 100 mL concentration was capable of improving the plant height, total of leaves, flowering period, and a total of pods with the respective average of 31.9 cm, 51.6 leaves, 21,8 days, and 35 soybean pods

Comparison of Nodular Efficiencies Between Partridge Pea and Soybean Using Acetylene Reduction

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 449f-450
Author(s):  
Lisa M. Barry ◽  
Michael N. Dana
Keyword(s):  
Growth Rate ◽  
Plant Size ◽  
Silica Sand ◽  
Soybean Plant ◽  
Fibrous Root ◽  
Constant Growth Rate ◽  
Soil Microbial ◽  
Soybean Plants ◽  
Nurse Crops ◽  
Partridge Pea

Legumes are grown as nurse crops in agriculture because they increase soil microbial life and productivity. Native legumes have potential in ecological restoration to mimic the benefits found in agriculture plus they enhance the restored ecosystem. This study was initiated to compare the growth rates, nodulation characteristics, and nitrogen fixation rates of a native versus a non-native legume. The two legumes were partridge pea (Cassia fasciculata); a native, wild, annual legume and soybean (Glycine max `Century Yellow); a domesticated, agricultural, annual legume native to Asia. Plants were grown for 11 weeks in pots containing silica sand and received a nitrogen-free Hoagland's nutrient solution. Beginning at week 12, plants were harvested weekly for four consecutive weeks. Nodulated root systems were exposed to acetylene gas and the resulting ethylene amounts were measured. The two legumes exhibited significant differences in nodule size and shape and plant growth rate. In soybean, nodules were large, spherical, and clustered around the taproot while in partridge pea, nodules were small, irregularly shaped, and spread throughout the fibrous root system. Soybean plants had a significantly faster growth rate at the onset of the experiment but partridge pea maintained a constant growth rate and eventually exceeded soybean plant size. In spite of these observed differences, partridge pea and soybean plants were equally efficient at reducing acetylene to ethylene. These results indicate partridge pea has the potential to produce as much nitrogen in the field as soybean. Native legumes such as partridge pea deserve further research to explore their use as nurse crops in agricultural or restoration regimes.

Radio-Susceptibility of Nasopharyngeal Carcinoma: Focus on Epstein- Barr Virus, MicroRNAs, Long Non-Coding RNAs and Circular RNAs

2020 ◽  
Vol 13 (3) ◽  
pp. 192-205 ◽  
Author(s):  
Fanghong Lei ◽  
Tongda Lei ◽  
Yun Huang ◽  
Mingxiu Yang ◽  
Mingchu Liao ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Regulatory Networks ◽  
Epstein Barr Virus ◽  
Molecular Mechanisms ◽  
Acquired Resistance ◽  
Treatment Strategies ◽  
Circular Rnas ◽  
Barr Virus ◽  
Non Coding Rnas ◽  
Epstein Barr

Nasopharyngeal carcinoma (NPC) is a type of head and neck cancer. As a neoplastic disorder, NPC is a highly malignant squamous cell carcinoma that is derived from the nasopharyngeal epithelium. NPC is radiosensitive; radiotherapy or radiotherapy combining with chemotherapy are the main treatment strategies. However, both modalities are usually accompanied by complications and acquired resistance to radiotherapy is a significant impediment to effective NPC therapy. Therefore, there is an urgent need to discover effective radio-sensitization and radio-resistance biomarkers for NPC. Recent studies have shown that Epstein-Barr virus (EBV)-encoded products, microRNAs (miRNAs), long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), which share several common signaling pathways, can function in radio-related NPC cells or tissues. Understanding these interconnected regulatory networks will reveal the details of NPC radiation sensitivity and resistance. In this review, we discuss and summarize the specific molecular mechanisms of NPC radio-sensitization and radio-resistance, focusing on EBV-encoded products, miRNAs, lncRNAs and circRNAs. This will provide a foundation for the discovery of more accurate, effective and specific markers related to NPC radiotherapy. EBVencoded products, miRNAs, lncRNAs and circRNAs have emerged as crucial molecules mediating the radio-susceptibility of NPC. This understanding will improve the clinical application of markers and inform the development of novel therapeutics for NPC.

scholarly journals Integrative Modelling of Gene Expression and Digital Phenotypes to Describe Senescence in Wheat

Genes ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 909
Author(s):  
Anyela Valentina Camargo Rodriguez
Keyword(s):  
Gene Expression ◽  
Regulatory Networks ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Computational Modelling ◽  
Plant Morphology ◽  
Reproductive Organs ◽  
Biological Trait ◽  
Cereal Grain ◽  
Plant Level

Senescence is the final stage of leaf development and is critical for plants’ fitness as nutrient relocation from leaves to reproductive organs takes place. Although senescence is key in nutrient relocation and yield determination in cereal grain production, there is limited understanding of the genetic and molecular mechanisms that control it in major staple crops such as wheat. Senescence is a highly orchestrated continuum of interacting pathways throughout the lifecycle of a plant. Levels of gene expression, morphogenesis, and phenotypic development all play key roles. Yet, most studies focus on a short window immediately after anthesis. This approach clearly leaves out key components controlling the activation, development, and modulation of the senescence pathway before anthesis, as well as during the later developmental stages, during which grain development continues. Here, a computational multiscale modelling approach integrates multi-omics developmental data to attempt to simulate senescence at the molecular and plant level. To recreate the senescence process in wheat, core principles were borrowed from Arabidopsis Thaliana, a more widely researched plant model. The resulted model describes temporal gene regulatory networks and their effect on plant morphology leading to senescence. Digital phenotypes generated from images using a phenomics platform were used to capture the dynamics of plant development. This work provides the basis for the application of computational modelling to advance understanding of the complex biological trait senescence. This supports the development of a predictive framework enabling its prediction in changing or extreme environmental conditions, with a view to targeted selection for optimal lifecycle duration for improving resilience to climate change.

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