Bacillus subtilis and Bacillus licheniformis reduce faecal protein catabolites concentration and odor in dogs

2019 ◽  
Author(s):  
Tais Bastos ◽  
Daniele Cristina de Lima ◽  
Camilla Mariane Menezes Souza ◽  
Alex Maiorka ◽  
Simone Gisele de Oliveira ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Pathogenic Bacteria ◽  
Control Diet ◽  
Metabolizable Energy ◽  
Adaptation Period ◽  
Beagle Dogs ◽  
Fermentation Products ◽  
Faecal Score ◽  
Nutrients Digestibility

Abstract Background: Direct-fed microbials (DFM), as Bacillus subtilis and Bacillus licheniformis, may improve gut functionality of the host by favoring non-pathogenic bacteria and reducing the formation of putrefactive compounds. The aim of this study was to assess the nutrients digestibility, faecal characteristics and products of intestinal fermentation in dogs fed diets with Bacillus subtilis and Bacillus licheniformis. Sixteen adult beagle dogs randomly distributed were used. Every eight dogs were fed with the control diet or the diet to which 62.5 g DFM (B. subtilis and B. licheniformis)/ton were added. Diets were provided during a twenty days adaptation period, followed by five days of total faecal collection. Nutrients digestibility and metabolizable energy of the diets, and faecal characteristics and products of dogs’ intestinal fermentation were assessed. Results: There were no differences in nutrients digestibility (P >0.05). The DFM supplementation, however, improved the faecal score and resulted in less fetid faeces (P <0.001). The DFM inclusion reduced (P <0.05) the biogenic amines concentration: putrescine, spermidine and cadaverine, and the concentration of phenols and quinoline. Conclusions: The use of B. subtillis and B. licheniformis as DFM reduce the concentration of nitrogen fermentation products in the faeces and faecal odor, but the digestibility of nutrients is not altered in dogs.

Bacillus subtilis and Bacillus licheniformis reduce faecal protein catabolites concentration and odour in dogs

2020 ◽  
Author(s):  
Tais Silvino Bastos ◽  
Daniele Cristina de Lima ◽  
Camilla Mariane Menezes Souza ◽  
Alex Maiorka ◽  
Simone Gisele de Oliveira ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Biogenic Amines ◽  
Bacillus Licheniformis ◽  
Pathogenic Bacteria ◽  
Control Diet ◽  
Nutrient Digestibility ◽  
Adaptation Period ◽  
Fermentation Products ◽  
Metabolisable Energy ◽  
Faecal Score

Abstract Background: Direct-fed microbials (DFM), such as Bacillus subtilis and Bacillus licheniformis , may improve gut functionality of the host by favouring non-pathogenic bacteria and reducing the formation of putrefactive compounds. The aim of this study was to assess the nutrient digestibility, faecal characteristics and intestinal-fermentation products in dogs fed diets with Bacillus subtilis and Bacillus licheniformis . Sixteen dogs were randomly divided into two groups. Every eight dogs were fed with the control diet or the diet with the addition of 62.5 g of DFM ( B. subtilis and B. licheniformis )/ton. Diets were provided throughout a twenty-day adaptation period, followed by five days of total faecal collection. Nutrient digestibility and the metabolisable energy of the diets, plus the dogs’ faecal characteristics and intestinal fermentation products were assessed. Results: There were no differences in nutrient digestibility ( P > 0.05). However, DFM supplementation improved faecal score and resulted in less fetid faeces ( P < 0.001). DFM inclusion reduced ( P < 0.05) the biogenic amines concentration: putrescine, spermidine and cadaverine, besides the concentration of phenols and quinoline. Conclusions: The use of B. subtillis and B. licheniformis as DFM reduce the concentration of nitrogen fermentation products in faeces and faecal odour, but the digestibility of nutrients is not altered in dogs.

scholarly journals Comparison of Ameliorative Effects between Probiotic and Biodegradable Bacillus subtilis on Zearalenone Toxicosis in Gilts

Toxins ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 882
Author(s):  
Wenqiang Shen ◽  
Yaojun Liu ◽  
Xinyue Zhang ◽  
Xiong Zhang ◽  
Xiaoping Rong ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Control Diet ◽  
Hormone Secretion ◽  
Basal Diet ◽  
Diet Group ◽  
Conversion Ratio ◽  
Feed Conversion ◽  
Negative Effects ◽  
Agent Group

This study was conducted to compare the potential ameliorative effects between probiotic Bacillus subtilis and biodegradable Bacillus subtilis on zearalenone (ZEN) toxicosis in gilts. Thirty-six Landrace×Yorkshire gilts (average BW = 64 kg) were randomly divided into four groups: (1) Normal control diet group (NC) fed the basal diet containing few ZEN (17.5 μg/kg); (2) ZEN contaminated group (ZC) fed the contaminated diet containing an exceeded limit dose of ZEN (about 300 μg/kg); (3) Probiotic agent group (PB) fed the ZC diet with added 5 × 109 CFU/kg of probiotic Bacillus subtilis ANSB010; (4) Biodegradable agent group (DA) fed the ZC diet with added 5 × 109 CFU/kg of biodegradable Bacillus subtilis ANSB01G. Results showed that Bacillus subtilis ANSB010 and ANSB01G isolated from broiler intestinal chyme had similar inhibitory activities against common pathogenic bacteria. In addition, the feed conversion ratio and the vulva size in DA group were significantly lower than ZC group (p < 0.05). The levels of IgG, IgM, IL-2 and TNFα in the ZC group were significantly higher than PB and DA groups (p < 0.05). The levels of estradiol and prolactin in the ZC group was significantly higher than those of the NC and DA groups (p < 0.05). Additionally, the residual ZEN in the feces of the ZC and PB groups were higher than those of the NC and DA groups (p < 0.05). In summary, the ZEN-contaminated diet had a damaging impact on growth performance, plasma immune function and hormone secretion of gilts. Although probiotic and biodegradable Bacillus subtilis have similar antimicrobial capacities, only biodegradable Bacillus subtilis could eliminate these negative effects through its biodegradable property to ZEN.

scholarly journals The use of probiotics based on strains of the genus Bacillus in quail feeding

2019 ◽  
Vol 49 (2) ◽  
pp. 70-76
Author(s):  
O. G. Merzlyakova ◽  
V. A. Rogachev
Keyword(s):  
Bacillus Subtilis ◽  
Weight Gain ◽  
Survival Rate ◽  
Bacillus Licheniformis ◽  
Control Diet ◽  
Live Weight ◽  
Growth Period ◽  
Feed Consumption ◽  
Control Group ◽  
Live Weight Gain

The use of trial specimens of probiotics based on strains of Bacillus subtilis, Bacillus licheniformis and their consortium was experimentally proved to be effective for quail feeding during their growth. The dynamics of live weight gain of quails was monitored. The experiment lasted for 60 days and was carried out in compliance with the standard methods. Quails that were studied were of the Japanese breed formed in four groups of 80 heads in each: one control group and three experimental ones at the age of one day. Quails were kept in a battery cage with all the required environment conditions met. All the groups were fed on the main (control) diet, developed taking into account the age and physiological characteristics of quails. However, birds of experimental groups 1-3 were additionally fed on probiotics based on strains Bacillus subtilis, Bacillus licheniformis and their consortium in the amount of 150 g/t of feed, respectively. The effect of new strains of probiotics and their consortium was tested during the study by the following parameters: the survival rate of birds, intensity of chicken growth, meat productivity and hematological indicators. The optimum variant of probiotics introduction into the diet of quails during their growth period was determined. The recommendations for the preparation of compound feeds with the use of probiotic supplements were developed. The chemical composition of quail feed and meat was studied in the biological laboratory by conventional methods of zootechnical analysis. The introduction of the consortium of strains Bacillus subtilis and Bacillus licheniformis in the amount of 150 g/t led to an increase in the survival rate of quails by 4.0%. The average daily live weight gain increased by 2.57%, feed consumption per one unit of produce decreased by 6.48%. When feeding quails on the consortium of probiotic strains Bacillus subtilis and Bacillus licheniformis in this rate, the mass of the eviscerated bird carcass increased by 3.61%, the protein content in the meat and its biological value increased by 1.06 and 11.11%, respectively. The hematological characteristics of the chickens remained within the physiological norm.

scholarly journals Digestibility and fecal characteristics of dogs fed with Bacillus subtilis in diet

2010 ◽  
Vol 40 (10) ◽  
pp. 2169-2173 ◽  
Author(s):  
Ananda Portella Félix ◽  
Marina Volanski Teixeira Netto ◽  
Fabiane Yukiko Murakami ◽  
Cleusa Bernardete Marcon de Brito ◽  
Simone Gisele de Oliveira ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Dry Matter ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Test Diet ◽  
Beagle Dogs ◽  
Ammonia Content ◽  
Tukey Test ◽  
Randomized Experimental Design ◽  
Fecal Score

Considering the benefice demonstrated by the modulating action of probiotics on the host intestinal microbiota, this study aimed to evaluate diet digestibility and fecal characteristics of dogs fed with diets supplemented with Bacillus subtilis (C-3102). Twelve young Beagle dogs were distributed in a completely randomized experimental design consisting of two treatments: diet with no addition or with the addition of 0.01% Bacillus subtilis (C-3102). Dogs passed through 25 days of adaptation to the diets, and five days of total feces collection. The following fecal characteristics were evaluated: pH, fecal score (1 - watery feces; 5: dry and hard feces), and ammonia content. Diet mean digestibility was compared by the Tukey test, and fecal characteristics by the Tukey-Kramer test. Diet digestibility was not different between treatments, but dogs supplemented with the tested probiotic presented dryer feces (39.1% vs. 36.5% dry matter), higher fecal score (3.4 vs. 3.0) and lower fecal ammonia content (0.45% vs. 0.56%), than dogs fed with the control diet. The dietary supplementation with Bacillus subtilis (C-3102) improves fecal texture and odor in dogs.

scholarly journals Net energy value of non-starch polysaccharide isolates (sugarbeet fibre and commercial inulin) and their impact on nutrient digestive utilization in healthy human subjects

1998 ◽  
Vol 80 (4) ◽  
pp. 343-352 ◽  
Author(s):  
C. Castiglia-Delavaud ◽  
E. Verdier ◽  
J. M. Besle ◽  
J. Vernet ◽  
Y. Boirie ◽  
...  
Keyword(s):  
Human Subjects ◽  
Control Diet ◽  
Latin Square ◽  
Metabolizable Energy ◽  
Whole Body ◽  
Net Energy ◽  
Adaptation Period ◽  
Healthy Human ◽  
Energy Value ◽  
Net Energy Value

The energy value of NSP has been expressed as their metabolizable energy (ME) content. The aim of the present study was to determine whether differences in ME and net energy (NE) contents were similar for insoluble and soluble NSP. Nine healthy young men were offered three diets according to a Latin-square design (3 × 3) with three repetitions: diet C (control), diet B (control + 50 g sugarbeet fibre/d) and diet I (control + 50 g commercial inulin/d). After a 16 d adaptation period to NSP isolate, food intake was controlled (duplicate meal method) and faeces and urine were collected for 8 d. A period of 60 h was devoted to measurement of energy expenditure (EE) by whole-body indirect calorimetry. NSP-isolate ingestion induced significant increases in the number of defecations and stool weight resulting from increases in water, DM and microbial mass excretion. After deduction of microbial N, differences in faecal N excretion between diets were not significantly different. Urinary N excretion was slightly decreased by sugarbeet fibre or commercial inulin ingestion but the N balances for the diets were not significantly different. Diet energy, N and lipid apparent digestibilities decreased by only 1–2 %. Commercial inulin was entirely fermented and fermentability of sugarbeet fibre averaged 0.886 (sd 0.117). Sugarbeet fibre and commercial inulin ME values averaged 10.7 (sd 1.2) and 13.0(sd 2.3) kJ/g DM respectively. NSP-isolate ingestion caused significant (sugarbeet) and nonsignificant (inulin) increases in daily EE. The maintenance NE contents of sugarbeet fibre and inulin averaged 5.0 (sd 5.0) and 11.9 (sd 1.3)kJ/g DM respectively. Differences in maintenance NE contents of NSP isolates were much greater than differences in ME values.

scholarly journals Old beagle dogs have lower faecal concentrations of some fermentation products and lower peripheral lymphocyte counts than young adult beagles

2011 ◽  
Vol 106 (S1) ◽  
pp. S187-S190 ◽  
Author(s):  
Márcia de Oliveira Sampaio Gomes ◽  
Mariana Casteleti Beraldo ◽  
Thaila Cristina Putarov ◽  
Márcio Antônio Brunetto ◽  
Leandro Zaine ◽  
...  
Keyword(s):  
Cell Wall ◽  
Young Adult ◽  
Yeast Cell ◽  
Peripheral Lymphocyte ◽  
Yeast Cell Wall ◽  
Cytotoxic Lymphocytes ◽  
Adaptation Period ◽  
Beagle Dogs ◽  
Fermentation Products ◽  
Mature Adult

The effects of age on microbiota composition, gut fermentation end-product formation and peripheral lymphocyte numbers were compared between old and young adult Beagle dogs fed four kibble diets differing in yeast cell wall contents. The experiment had a double 4 × 4 Latin square design, one with four mature dogs (4 years old) and the other with four old dogs (10 years old), with four replicates (diets) per dog. In each period a 15 d adaptation period preceded a 5 d total collection of faeces for the digestibility trial. On day 21, fresh faecal samples were collected for the determination of bacterial enumeration, pH, biogenic amine and short-chain fatty acid. Flow cytometry was used for immunophenotypic evaluation. Dogs were fed four kibble diets with similar composition with 0, 0·15, 0·30 and 0·45 % of yeast cell wall (as-fed), respectively. Data were evaluated using general linear models of Statistical Analysis Systems statistical software (P < 0·05). No evidence of a difference in faecal bacteria counts between ages was found (total aerobes, total anaerobes,Bifidobacterium, Lactobacillus, ClostridiumandEscherichia coli:P>0·15). Faecal concentrations of butyrate, histamine, agmatine and spermine were lower (P ≤ 0·05) and faecal pH was higher (P = 0·03) in older dogs than in mature adult dogs, suggesting an alteration in bacterial metabolic activity, or in the rate of intestinal absorption of these compounds. Concentrations of T-lymphocytes, T-cytotoxic lymphocytes and B-lymphocytes were also lower (P ≤ 0·01) in older dogs than in mature adult dogs. The study confirmed alterations in peripheral lymphocytes and revealed a reduced concentration of some fermentation end products in the colon of old dogs.

ANTIMICROBIAL ACTIVITIES OF METHANOL EXTRACT FROM UNIDENTIFIED SPONGE ASSOCIATED BACTERIA IN LEMUKUTAN ISLAND, KALIMANTAN BARAT

2009 ◽  
Vol 1 (2) ◽  
Author(s):  
Risa Nofiani ◽  
Siti Nurbetty ◽  
Ajuk Sapar
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Agar Medium ◽  
Antimicrobial Activities ◽  
Antimicrobial Compounds ◽  
Associated Bacteria ◽  
Minimum Inhibitory Concentrations ◽  
Sponge Associated Bacteria

<p>The increase of issues on the antibiotics resistant pathogenic bacteria has triggered high exploration for new antimicrobial compounds. One of the potential sources is sponge-associated bacteria. The aim of this study was to get sponge-associated bacteria extract containing antimicrobial activities. On the basis screening of antimicrobial activity using by streaking on agar medium, there were two potential isolates with antimicrobial activities namely LCS1 and LCS2. The two isolates were cultivated,then secondary metabolite product were extracted using methanol as a solvent. Minimum inhibitory concentrations (MICs) of extract LCS 1 were 1,000 μg/well for S. aureus, 950 μg/well for Salmonella sp.and 800 μg/well for Bacillus subtilis. Minimum inhibitory concentrations of extract LCS 2 were 500 μg/well for S. aureus, 1,050 μg/well for Salmonella sp., 750 μg/well for Bacillus subtilis, 350 μg/well for P. aeruginosa, 750 μg/sumur terhadap B. subtilis. Based on the MIC values, the two assay extracts have a relatively low antimicrobial activity.</p> <p>Keywords:Antimicrobial,Sponges associated bacteria,MICs</p>

Probiotics in the diets of laying quails

2020 ◽  
pp. 60-66
Author(s):  
O. Merzlyakova ◽  
V. Rogachyev ◽  
V. Chegodaev
Keyword(s):  
Bacillus Subtilis ◽  
Bacillus Licheniformis ◽  
Egg Production ◽  
Positive Impact ◽  
Hematological Parameters ◽  
Economic Effect ◽  
Egg Laying ◽  
Control Group ◽  
Egg Mass ◽  
The Cost

The efficiency of introducing probiotics based on strains of Bacillus subtilis, Bacillus licheniformis and their consortium in the amount of 150 g/t of feed into the diets of laying quails has been studied. The experiment lasting 182 days has been carried out on four groups of quails with 30 heads in each. The quails have been housed in the broiler battery in compliance with the required microclimate conditions. Quails of all groups have been received the main diet (compound feed) developed taking into account their age and physiological characteristics. The quails of the 1st, 2nd and 3rd experimental groups in addition to the main diet received probiotics (150 g/t compound feed) based on strains Bacillus subtilis, Bacillus licheniformis and their consortium, respectively. It has been found that feeding the laying quails of the consortium of strains Bacillus subtilis and Bacillus licheniformis had the most significant positive impact on their productive performance, it allowed to increase egg production by 7,81 %, egg laying intensity by 5,0 %, egg mass yield by 9,77 %, while reducing feed expenditures for 10 eggs by 13,35 %. The yield of hatching eggs has been increased by 7,03 %, hatchability of chickens from laid and fertilized eggs by 8,33 and 8,35 %, brooding waste decreased by 21,74 %. Hematological parameters of quails during the whole experiment were within the physiological norm. The economic effect calculated on the basis of data on the cost of compound feed, probiotics and the cost of sold eggs of quail laying was 14,56 % in the 3rd experimental group (in relation to the control group).

scholarly journals Effect of BioPlus YC Probiotic Supplementation on Gut Microbiota, Production Performance, Carcass and Meat Quality of Pigs

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1581
Author(s):  
Artur Rybarczyk ◽  
Elżbieta Bogusławska-Wąs ◽  
Alicja Dłubała
Keyword(s):  
Bacillus Subtilis ◽  
Meat Quality ◽  
Bacillus Licheniformis ◽  
Average Daily Gain ◽  
Production Performance ◽  
Probiotic Bacteria ◽  
Control Group ◽  
Production Traits ◽  
Feed Conversion

The objective of the study was to determine the effects of probiotic bacteria Bacillus licheniformis and Bacillus subtilis on microbiological properties of feed mixtures and on the digestive tract content as applicable to production traits and carcass characteristics of fatteners. The experiment was performed on 83,838 fatteners from four successive (insertions) productions in two groups. From the seventy eighth day of age till marketing to the slaughter plant, the pigs were supplied with BioPlus YC probiotic (Chr. Hansen) in the amount of 400 g/t. The preparation contained a complex of probiotic bacteria Bacillus licheniformis DSM 5749, and Bacillus subtilis DSM 5750 spores in a 1:1 ratio. From the fourth insertion, after reaching a body weight of approximately 112 kg, 60 fatteners were selected from each group to measure carcass quality and half of them for meat quality evaluation. Moreover, microbiological analyses in feed and colon were performed. The study showed that BioPlus YC probiotics supplementation resulted in a significantly higher count of B. subtilis and B. licheniformis in the feed, a higher count of B. subtilis, B. licheniformis and LAB, as well as a lower count of Enterobacteriaceae, Enterococcus, Clostridium and Bacillus sp. in the mucosa and in the colorectal content of the test pigs. Our work has shown that supplementation with the BioPlus YC probiotic had a positive effect on the production traits of pigs mainly by reducing mortality (2.83%, p = 0.010), lowering feed conversion ratio—FCR (2.59 kg/kg, p = 0.013), better average daily gain—ADG (0.95 kg/day, p = 0.002) and shorter fattening period (77.25 days, p = 0.019) when compared to the control group (4.19%; 2.79 kg/kg; 0.89 kg/day; 92.8 days, respectively). The addition of the specific Bacillus bacteria did not influence carcass and meat characteristics of the test fatteners.

Sign in / Sign up

Export Citation Format

Share Document