scholarly journals Plasma Exo-miRNAs Correlated with AD-Related Factors of Chinese Individuals Involved in Aβ Accumulation and Cognition Decline

2021 ◽  
Author(s):  
Lifang WANG ◽  
Hefu Zhen ◽  
Yuzhe Sun ◽  
Shuang Rong ◽  
Benchao Li ◽  
...  
Keyword(s):  
Risk Factors ◽  
Mirna Expression ◽  
Leisure Activity ◽  
Mirna Expression Profile ◽  
Chinese Patients ◽  
Social Connection ◽  
Related Factors ◽  
Clinical Observations ◽  
Differentially Expressed Mirnas ◽  
Low Body Mass Index

Abstract Numerous studies have investigated the risk factors of Alzheimer’s disease (AD), however, AD- risk factors related miRNAs were rarely reported. In this study, AD- risk factor related miRNAs of 119 Chinese individuals (47 AD patients and 62 cognitively normal controls) were investigated. The results showed that education, tea consumption, leisure activity, and social connection were low Body Mass Index (BMI), depression, and smoking were potentially risk factors for AD patients. Moreover, we screened exosome microRNAs (miRNAs) for the aforementioned lifestyle-related factors. Canonical correlation analyses revealed that our clinical observations were correlated with the miRNA expression profile for education, depression, leisure activity, and social connection. Furthermore, we predicted the targets of differentially expressed miRNAs and found that many of them were reported to be involved in the generation and clearance of Amyloid-beta (Aβ), important molecules related to cognition, and disease-activated microglia response to AD. Our results indicated that certain education and depression factors can contribute to AD progression by modulating miRNA expression, implying that preventive interventions might alter AD progression in Chinese patients.

scholarly journals Determinants of Brachial-Ankle Pulse Wave Velocity in Chinese Patients with Rheumatoid Arthritis

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ping Li ◽  
Cheng-xun Han ◽  
Cui-li Ma ◽  
Jia-long Guo ◽  
Bo Liu ◽  
...  
Keyword(s):  
Risk Factors ◽  
Pulse Wave Velocity ◽  
Wave Velocity ◽  
Serum Cholesterol ◽  
Pulse Wave ◽  
Multivariate Regression Analysis ◽  
Chinese Patients ◽  
Control Group ◽  
Related Factors ◽  
Serum Triglycerides

Objective. To investigate the relationship between Brachial-ankle pulse wave velocity (baPWV), and its associated risk factors in Chinese patients with RA.Methods. 138 Chinese RA patients and 150 healthy subjects were included. baPWV of all the participants was measured. RA related factors were determined, as well as traditional cardiovascular risk factors.Results. baPWV was significant higher in RA group (1705.44 ± 429.20 cm/s) compared to the healthy control group (1386.23 ± 411.09 cm/s) (P<0.001). Compared with low baPWV group, high baPWV group patients were significantly older (P= 0.008) and taller (P= 0.033). Serum cholesterol (P= 0.035), triglycerides (P= 0.004), and LDL level (P= 0.006) were significantly higher in high baPWV group patients compared with low baPWV group patients. The baPWV of RA patients was positively correlated with age (r= 0.439,P<0.001), and serum cholesterol level (r= 0.231,P= 0.035), serum triglycerides level (r= 0.293,P<0.001), serum LDL level (r= 0.323,P= 0.003). Meanwhile, baPWV negatively correlated with the height of RA patients (r= −0.253,P= 0.043). Multivariate regression analysis showed that baPWV of RA group was independently associated with age and serum triglycerides level.Conclusions. The old age and high level of serum triglycerides may be the major determinants of arterial stiffness in Chinese RA patients.

scholarly journals MicroRNA expression patterns associated with hyperfunctioning and non-hyperfunctioning phenotypes in adrenocortical adenomas

2014 ◽  
Vol 170 (4) ◽  
pp. 583-591 ◽  
Author(s):  
David Velázquez-Fernández ◽  
Stefano Caramuta ◽  
Deniz M Özata ◽  
Ming Lu ◽  
Anders Höög ◽  
...  
Keyword(s):  
Mirna Expression ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Gene Mutations ◽  
Mirna Expression Profile ◽  
Differentially Expressed ◽  
Adrenocortical Adenoma ◽  
Tumor Subtypes ◽  
Mirna Expression Profiles ◽  
Differentially Expressed Mirnas

BackgroundThe adrenocortical adenoma (ACA) entity includes aldosterone-producing adenoma (APA), cortisol-producing adenoma (CPA), and non-hyperfunctioning adenoma (NHFA) phenotypes. While gene mutations and mRNA expression profiles have been partly characterized, less is known about the alterations involving microRNA (miRNA) expression.AimTo characterize miRNA expression profile in relation to the subtypes of ACAs.Subjects and methodsmiRNA expression profiles were determined in 26 ACAs (nine APAs, ten CPAs, and seven NHFAs) and four adrenal references using microarray-based screening. Significance analysis of microarrays (SAM) was carried out to identify differentially expressed miRNAs between ACA and adrenal cortices or between tumor subtypes. Selected differentially expressed miRNAs were validated in an extended series of 43 ACAs and ten adrenal references by quantitative RT-PCR.ResultsAn hierarchical clustering revealed separate clusters for APAs and CPAs, while the NHFAs were found spread out within the APA/CPA clusters. When NHFA was excluded, the clustering analysis showed a better separation between APA and CPA. SAM analysis identified 40 over-expressed and three under-expressed miRNAs in the adenomas as compared with adrenal references. Fourteen miRNAs were common among the three ACA subtypes. Furthermore, we found specific miRNAs associated with different tumor phenotypes.ConclusionThe results suggest that miRNA expression profiles can distinguish different subtypes of ACA, which may contribute to a deeper understanding of ACA development and potential therapeutics.

scholarly journals miRNA Expression Profile and Effect of Wenxin Granule in Rats with Ligation-Induced Myocardial Infarction

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Aiming Wu ◽  
Lixia Lou ◽  
Jianying Zhai ◽  
Dongmei Zhang ◽  
Limin Chai ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Expression Profile ◽  
Mirna Expression ◽  
Mirna Expression Profile ◽  
Differentially Expressed ◽  
Coronary Artery Ligation ◽  
Mrna Levels ◽  
Artery Ligation ◽  
Gene Level ◽  
Differentially Expressed Mirnas

Wenxin Granule (WXKL) is a traditional Chinese medicine used for treatment of myocardial infarction (MI) and arrhythmias. However, the genomic pathological mechanisms of MI and mechanisms of WXKL are largely unknown. This study aims to investigate a comprehensive miRNA expression profile, and the predicted correlation pathways to be targeted by differentially expressed miRNAs in MI, and mechanisms of WXKL from a gene level. MI rat model was established by a coronary artery ligation surgery. miRNA expression microarrays were performed and the data were deposited in Gene Expression Omnibus (GEO number GSE95855). And, pathway analysis was performed by using the DIANA-miRPath v3.0 online tool. The expressions of miR-1, miR-133, Cx43, and Cx45 were detected by quantitative real-time PCR. It was found that 35 differentially expressed miRNAs and 23 predicted pathways, including miR-1, miR-133, and gap junction pathway, are involved in the pathogenesis of MI. And, WXKL increased the expressions of miR-1 and miR-133, while also increased the mRNA levels of Cx43 and Cx45, and, especially, recovered the Cx43/Cx45 ratio near to normal level. The results suggest that regulatory effects on miR-1, miR-133, Cx43, and Cx45 might be a possible mechanism of WXKL in the treatment of MI at the gene level.

scholarly journals miRNA Expression Profile of Saliva in Subjects of Yang Deficiency Constitution and Yin Deficiency Constitution

2018 ◽  
Vol 49 (5) ◽  
pp. 2088-2098 ◽  
Author(s):  
Yu Chen ◽  
Yanling Wu ◽  
Haiqiang Yao ◽  
Hui Luo ◽  
Bing Lin ◽  
...  
Keyword(s):  
Expression Profile ◽  
Mirna Expression ◽  
Target Genes ◽  
Mirna Expression Profile ◽  
Differentially Expressed ◽  
Hormone Signaling ◽  
Chinese Han ◽  
Cold Intolerance ◽  
Yin Deficiency ◽  
Differentially Expressed Mirnas

Background/Aims: Based on the theory of constitution in Traditional Chinese Medicine (TCM), the Chinese Han population has been classified into nine constitutions. Of these, Yang deficiency constitution mainly exhibit cold intolerance while Yin deficiency constitution mainly exhibit heat intolerance. Some studies have been carried out to explore the modern genetic and biological basis of such constitution classification, but more remains to be done. MicroRNA (miRNA) serves as post-transcriptional regulators of gene expression and may play a role in the classification process. Here, we examined miRNA expression profile of saliva to further improve the comprehensiveness of constitution classification. Methods: Saliva was collected from Chinese Han individuals with Yang deficiency, Yin deficiency and Balanced constitutions (n=5 each), and miRNA expression profile was determined using the Human miRNA OneArray®v7. Based on 1.5 Fold change, means log2|Ratio|≥0.585 and P-value< 0.05, differentially expressed miRNA was screened. Target genes were predicted using DIANA-TarBasev7.0 and analysis of KEGG pathway was carried out using DIANA-mirPathv.3. Results: We found that 81 and 98 differentially expressed miRNAs were screened in Yang deficiency and Yin deficiency constitution, respectively. Among them, 16 miRNAs were identical and the others were unique. In addition, the target genes that are regulated by the unique miRNAs were significantly enriched in 27 and 20 signaling pathways in Yang deficiency and Yin deficiency constitution, respectively. Thyroid hormone signaling pathway is present in both constitutions. These unique miRNAs that regulated target genes of thyroid hormone signaling pathway may be associated with cold intolerance or heat intolerance. Conclusion: The results of our study show that Yang deficiency and Yin deficiency constitutions exhibit systematic differences in miRNA expression profile. Moreover, the distinct characteristics of TCM constitution may be explained, in part, by differentially expressed miRNAs.

scholarly journals Comparison of miRNA Expression Profiles between HIV-1 and HIV-2 Infected Monocyte-Derived Macrophages (MDMs) and Peripheral Blood Mononuclear Cells (PBMCs)

2020 ◽  
Vol 21 (18) ◽  
pp. 6970
Author(s):  
Santanu Biswas ◽  
Emily Chen ◽  
Mohan Haleyurgirisetty ◽  
Sherwin Lee ◽  
Indira Hewlett ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Signaling Pathways ◽  
Differential Expression ◽  
Signaling Pathway ◽  
Mirna Expression ◽  
Expression Profiles ◽  
Mirna Expression Profile ◽  
Differentially Expressed Mirnas ◽  
Hiv 1

During the progression of HIV-1 infection, macrophage tropic HIV-1 that use the CCR5 co-receptor undergoes a change in co-receptor use to CXCR4 that is predominately T cell tropic. This change in co-receptor preference makes the virus able to infect T cells. HIV-2 is known to infect MDMs and T cells and is dual tropic. The aim of this study was to elucidate the differential expression profiles of host miRNAs and their role in cells infected with HIV-1/HIV-2. To achieve this goal, a comparative global miRNA expression profile was determined in human PBMCs and MDMs infected with HIV-1/HIV-2. Differentially expressed miRNAs were identified in HIV-1/HIV-2 infected PBMCs and MDMs using the next-generation sequencing (NGS) technique. A comparative global miRNA expression profile in infected MDMs and PBMCs with HIV-1 and HIV-2 identified differential expression of several host miRNAs. These differentially expressed miRNAs are likely to be involved in many signaling pathways, like the p53 signaling pathway, PI3K-Akt signaling pathways, MAPK signaling pathways, FoxO signaling pathway, and viral carcinogenesis. Thus, a comparative study of the differential expression of host miRNAs in MDMs and T cell in response to HIV-1 and HIV-2 infection will help us to identify unique biomarkers that can differentiate HIV-1 and HIV-2 infection.

scholarly journals Factors Associated with Diabetic Retinopathy in Chinese Patients with Type 2 Diabetes Mellitus

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Bin-Bin He ◽  
Li Wei ◽  
Yun-Juan Gu ◽  
Jun-Feng Han ◽  
Ming Li ◽  
...  
Keyword(s):  
Risk Factors ◽  
Blood Pressure ◽  
Logistic Regression ◽  
Diabetic Retinopathy ◽  
Glycated Albumin ◽  
Chinese Patients ◽  
Related Factors ◽  
C Peptide ◽  
Duration Of Diabetes ◽  
Factors Associated

Objective.To investigate the risk factors of DR in Chinese T2DM patients.Methods.2009 patients with T2DM were included in this cross-sectional study. All patients underwent eye examination, and the DR stage was defined by an ophthalmologist. Correlation analysis was performed to evaluate the relation between DR and clinical variables. Logistic regression models were used to assess risk for those factors associated with DR.Results.A total of 597 T2DM patients (29.7%) had DR, of which 548 (27.3%) were nonproliferative diabetic retinopathy and 49 (2.4%) were proliferative diabetic retinopathy. Positive correlations were found between DR and duration of diabetes, systolic blood pressure (SBP), diastolic blood pressure, glycated hemoglobin, glycated albumin, 24 hurinary albumin excretion, peripheral atherosclerosis (PA), diabetes nephropathy (DN), diabetic peripheral neuropathy, and anemia. Negative correlations were found between DR and C-peptide and glomerular filtration rate. Logistic regression analysis revealed that duration of diabetes, SBP, DN, anemia, PA, and C-peptide were each independent risk factors of DR.Conclusion.The duration of diabetes, SBP, DN, anemia, and PA are positively associated with DR in Chinese T2DM patients, while C-peptide is negatively associated with DR. Monitoring and evaluation of these related factors will likely contribute to the prevention and treatment of DR.

scholarly journals Serum exosomal miRNA from endometriosis patients correlates with disease severity

2021 ◽  
Author(s):  
Yahong Wu ◽  
Wen Yuan ◽  
Hui Ding ◽  
Xianqing Wu
Keyword(s):  
Mirna Expression ◽  
Mirna Expression Profile ◽  
Differentially Expressed ◽  
Pcr Analysis ◽  
Marker Proteins ◽  
Differentially Expressed Mirnas ◽  
Mirna Expression Profiling ◽  
Exosomal Mirna ◽  
Serum Exosomes ◽  
Normal Controls

Abstract Purpose Exosomes are vesicles secreted by cells that contain a wide variety of biomolecules, including proteins or nucleic acids. MicroRNAs (miRNAs), which are commonly found in exosomes, are known to play important roles in the pathophysiology of endometriosis. Methods This study investigated the miRNA expression profile of serum exosomes from women with endometriosis in comparison with normal controls as well as the possible role of identified miRNAs in the pathogenesis of endometriosis. Exosomes with a diameter between 60 and 100 nm were identified by their expression of exosomal marker proteins CD9 and CD63. Results Microarray miRNA expression profiling analysis revealed that 26 genes were significantly up-regulated and 19 genes were significantly down-regulated in serum exosomes from endometriosis patients compared with normal controls. These differentially expressed miRNAs were mainly enriched in the regulation of cellular development, metabolism, and involved in the regulation of the MAPK and PI3k-Akt pathways. qRT-PCR analysis verified the differential expression of three miRNAs, miR-26b-5p, miR-215-5p, and miR-6795-3p. Conclusion Further analysis indicated that these differentially expressed miRNAs in serum exosomes may be involved in the pathogenesis of endometriosis and are related to the severity and certain symptoms of endometriosis.

scholarly journals Cardiac miRNA Expression and their mRNA Targets in a Rat Model of Prediabetes

2020 ◽  
Vol 21 (6) ◽  
pp. 2128
Author(s):  
Éva Sághy ◽  
Imre Vörös ◽  
Bence Ágg ◽  
Bernadett Kiss ◽  
Gábor Koncsos ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Mirna Expression ◽  
Drug Targets ◽  
Zinc Finger Protein ◽  
Target Prediction ◽  
Mirna Expression Profile ◽  
Differentially Expressed ◽  
Small Rna Sequencing ◽  
Mrna Targets ◽  
Differentially Expressed Mirnas

Little is known about the mechanism of prediabetes-induced cardiac dysfunction. Therefore, we aimed to explore key molecular changes with transcriptomic and bioinformatics approaches in a prediabetes model showing heart failure with preserved ejection fraction phenotype. To induce prediabetes, Long-Evans rats were fed a high-fat diet for 21 weeks and treated with a single low-dose streptozotocin at week 4. Small RNA-sequencing, in silico microRNA (miRNA)-mRNA target prediction, Gene Ontology analysis, and target validation with qRT-PCR were performed in left ventricle samples. From the miRBase-annotated 752 mature miRNA sequences expression of 356 miRNAs was detectable. We identified two upregulated and three downregulated miRNAs in the prediabetic group. We predicted 445 mRNA targets of the five differentially expressed miRNAs and selected 11 mRNAs targeted by three differentially expressed miRNAs, out of which five mRNAs were selected for validation. Out of these five targets, downregulation of three mRNAs i.e., Juxtaposed with another zinc finger protein 1 (Jazf1); RAP2C, member of RAS oncogene family (Rap2c); and Zinc finger with KRAB and SCAN domains 1 (Zkscan1) were validated. This is the first demonstration that prediabetes alters cardiac miRNA expression profile. Predicted targets of differentially expressed miRNAs include Jazf1, Zkscan1, and Rap2c mRNAs. These transcriptomic changes may contribute to the diastolic dysfunction and may serve as drug targets.

Critical Review on Suicide Among Nurses

Crisis ◽  
2015 ◽  
Vol 36 (2) ◽  
pp. 91-101 ◽  
Author(s):  
Marie Alderson ◽  
Xavier Parent-Rocheleau ◽  
Brian Mishara
Keyword(s):  
Risk Factors ◽  
Literature Review ◽  
High Stress ◽  
Related Factors ◽  
Work Settings ◽  
Work Related ◽  
High Prevalence ◽  
Full Text Review ◽  
The Subject ◽  
Psychiatric Problems

Background: Research shows that there is a high prevalence of suicide among nurses. Despite this, it has been 15 years since the last literature review on the subject was published. Aim: The aim of this article is to review the knowledge currently available on the risk of suicide among nurses and on contributory risk factors. Method: A search was conducted in electronic databases using keywords related to prevalence and risk factors of suicide among nurses. The abstracts were analyzed by reviewers according to selection criteria. Selected articles were submitted to a full-text review and their key elements were summarized. Results: Only nine articles were eligible for inclusion in this review. The results of this literature review highlight both the troubling high prevalence of suicide among nurses as well as the persistent lack of studies that examine this issue. Conclusion: Considering that the effects of several factors related to nurses' work and work settings are associated with high stress, distress, or psychiatric problems, we highlight the relevance of investigating work-related factors associated with nurses' risk of suicide. Several avenues for future studies are discussed as well as possible research methods.

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