Antimicrobial Activities of Fungus Comb Extracts Isolated From Indo-Malayan Termite Macrotermes Gilvus Mound

Abstract The embodiment of antimicrobial components into the food packaging material has the ability to prevent microbial contamination. Fungus comb could be an alternative source of natural antimicrobial agents. In this study, n-hexane, ethyl acetate, methanol, and water extracts from fungus comb isolated from Indo-malayan termite Macrotermes gilvus Hagen mound were analysed for its antibacterial and antifungal activities against food spoilage microorganisms, including Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Aspergillus flavus, and Aspergillus niger. The antimicrobial activity of the fungus comb extracts was evaluated using Kirby-Bauer disc diffusion and microdilution method. The result showed that ethyl acetate extract exhibited the biggest diameter inhibition zone for all bacteria and fungi tested. Ethyl acetate extract showed antibacterial activity in all bacteria with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.39 mg/mL and 0.78 mg/mL, respectively. This extract also inhibited A. flavus and A. niger with MIC value of 0.78 mg/ml. Ethyl acetate extract contained guaiacol and syringol, which were predicted as the main antimicrobial components in fungus comb. Whereas n-hexane extract only inhibited Gram-positive bacteria. S. aureus ATCC 25923 was the most sensitive bacteria tested using all extracts. In addition, A. flavus was more sensitive compared to A. niger. Overall, fungus comb extract exhibited antimicrobial activity against E.coli ATCC 25922, P. aeruginosa ATCC 27853, S. aureus ATCC 25923, A. flavus, and A. niger. This study revealed that the fungus comb extract, especially ethyl acetate, could be considered as a new antimicrobial agent.

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A preliminary study on the antimicrobial activities and gas chromatography-mass spectrometry (GC-MS) analysis of the ethyl acetate extract of Dennettia tripetala G. Baker seeds

Bio-Research ◽

10.4314/br.v18i1.4 ◽

2020 ◽

Vol 18 (1) ◽

pp. 1094-1102

Author(s):

UF Babaiwa ◽

SO Eraga ◽

EO Ojugo ◽

O Erharuyi ◽

JO Akerele

Keyword(s):

Antimicrobial Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Chemical Constituents ◽

Antimicrobial Activities ◽

Fatty Acid Esters ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Traditional Use ◽

Dennettia Tripetala

The study investigated the antimicrobial properties and the chemical composition of ethyl acetate extract of Dennettia tripetala (pepper fruit) seeds. Crude extract obtained by maceration of pulverized seeds in ethyl acetate was evaluated for antimicrobial activity against Escherichia coli, Staphylococcus aureus, Klebsiella aerogenes, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans and Aspergillus niger using standard agar-well diffusion method. GC-MS method was used to determine the chemical constituents of the extract. The extract was oily, yellowish-brown with a yield of 1.66 % and had activity against most of the test microorganisms, with inhibition zone diameters ranging between 10 to 25 mm. About 41 chemical constituents were present in the extract with formic acid methyl esters and fatty acids accounting for 57.23 and 18.49 % respectively. Ethyl acetate extract of Dennettia tripetala seeds possessed antimicrobial activity against bacteria but not fungi. The observed activity may be due to the presence of formic and fatty acid esters in the seed. The study further established a scientific proof for the traditional use of Dennettia tripetala seed extracts in treating microbial infections.

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Antimicrobial potentials, phytochemical screening and determination of bioactive compounds from Three Indian folk medicinal plants

Current Bioactive Compounds ◽

10.2174/1573407216999200719233247 ◽

2020 ◽

Vol 16 ◽

Author(s):

V. Vanitha ◽

S. Vijayakumar ◽

S. Rajalakshmi

Keyword(s):

Antimicrobial Activity ◽

Ethyl Acetate ◽

Leaf Extract ◽

Antimicrobial Agents ◽

Ethyl Acetate Extract ◽

Diffusion Method ◽

Dilution Method ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Ft Ir

Aim: To investigate the antimicrobial efficacy of various solvents leaf extracts of Aegle marmelos, Plumbago zeylanica and Rhinacanthus nasutus against human pathogenic organisms such as Acinetobacter baumannii, Salmonella enteriditis, Bacillus cereus, Streptococcus pneumonia, Mycobacterium tuberculosis and Aspergillus fumigatous by agar well diffusion method. Background: Also, this is the first time report on MIC and MBC/MFC activities and identify the phytochemicals and functional groups using HPLC, GC-MS and FT-IR analysis. Objective: Minimum inhibitory concentration (MIC), minimum bactericidal (MBC/MFC) values were also carried out by the micro dilution method. Methods: Ethyl acetate extract of P. zeylanica showed better antimicrobial activity against the tested pathogens, especially for S. pneumoniae (40 mm) followed by ethyl acetate extract of R. nasutus (36 mm) against S. pneumoniae. While least inhibition was observed for aqueous extract of P.zeylanica against S. enteritis (10 mm). Results: The MIC ranged from 3.75 µg/ml to 60µg/ml and MBC/MFC 7.5 µg/ml to 60 µg/ml. Phytochemical analysis exhibited the presence of alkaloids, steroids, saponins, flavonoids, tannins, terpenes, phenolics and cardiac glycoside in all samples of selected plant extracts. Tannins, phenolics and glycoside were found only in the ethyl acetate extract of P.zeylanica. HPLC and GC-MS analysis of ethyl acetate leaf extract of P. zeylanica exposed ten major peaks and eleven compounds respectively. Chemical nature of the leaf extract was analysed by FT-IR spectrum. Conclusion: Ethyl acetate leaf extract of P. zeylanica showed better antimicrobial activity than other extracts as well as standard drugs. Therefore, this plant could be studied in detail for the identification of active biomolecules for antimicrobial agents.

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Studies on comparative antimicrobial activities of Aerva lanata and Momordica charantia leaf extracts

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2020.120519 ◽

2020 ◽

pp. 561-564

Author(s):

Nagula Shilpika ◽

Byagari Teena Keerthana ◽

Banka Srinivas ◽

Gampa Tulja Rani ◽

Ganga Maithili

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Antimicrobial Activities ◽

Momordica Charantia ◽

Leaf Extracts ◽

Plate Method ◽

Methanolic Extracts ◽

Aerva Lanata

Screening and comparison of antimicrobial action of leaf extract of Aerva lanata and Momordica charantia. Ethyl acetate and methanolic extracts of leaves of plants were screened for antimicrobial activity using the cup plate method and the spread plate method against gram positive and gram negative reference organisms (Bacillus substilis and Escherichia coli).The standard antibacterial agent used for reference is Choramphenicol and the results were calculated as zone of inhibition. Methanolic extract showed comparatively broader and better antimicrobial spectrum than ethyl acetate extract in selected plants. Plant extracts showed dose dependent action, results were similar to the action of the standard Choramphenicol. Extracts of Aerva lanata and Momordica charantia demonstrated antimicrobial activity on tested microorganisms. Methanolic extracts showed higher antimicrobial potential than ethyl acetate extract. Aerva lanata extracts showed better response than Momordica charantia extracts in the cup plate method antibacterial activity with Bacillus substilis and Escherichia coli.

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Antimicrobial Activity of Allium chinense G. Don.

Current Biochemistry ◽

10.29244/cb.2.3.129-138 ◽

2017 ◽

Vol 2 (3) ◽

pp. 129-138

Author(s):

Frans Grovy Naibaho ◽

Maria Bintang ◽

Fachriyan Hasmi Pasaribu

Keyword(s):

Antimicrobial Activity ◽

Ethyl Acetate ◽

Microbial Growth ◽

Inhibitory Concentration ◽

Ethyl Acetate Extract ◽

Salmonella Typhi ◽

Antimicrobial Activities ◽

Biologically Active ◽

E Coli ◽

Allium Chinense

This study aims to analyze antimicrobial activity of Allium chinense G. Don extract against Eschericia coli, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis and Candida albicans, and to examine the active compounds. Allium chinense G. Don was extracted using maseration method and treated with ethanol 70% (v/v), ethanol 96% (v/v), ethyl acetate, n-hexane, and aquadest. Antimicrobial activity assay was conducted using agar difusion method and compounds analysis using Gas chromatography-massspectrometry (GC-MS). Antimicrobial activities assay showed that all extracts could inhibit microbial growth. Ethyl acetate extract has the highest antimicrobial activity against all the microbial test. Minimum inhibitory concentration (MIC) of ethyl acetate extract against C. albicans, B. subtilis, E. coli, S. aureus, S. typhi was 25, 100, 250, 250, 1000 mg ml-1 respectively. As many as 25 compounds were derived from GC-MS analysis and most of them were known as the antimicrobial compounds. This study revealed that Allium chinense G. Don contains biologically active compunds as antimicrobial agent particularly anti Candida.

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Antimicrobial activity of n-hexane and Ethyl acetate extracts of Erythrina stricta Roxb

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v27i2.9176 ◽

1970 ◽

Vol 27 (2) ◽

pp. 65-66

Author(s):

Mohammad Musarraf Hussain ◽

M Mizanur Rahman Mughal ◽

Md Masud Alam ◽

Mohammad Golam Dastagir ◽

AHM Masum Billah ◽

...

Keyword(s):

Antimicrobial Activity ◽

Ethyl Acetate ◽

Key Words ◽

Microbial Growth ◽

Ethyl Acetate Extract ◽

Stem Bark ◽

Antimicrobial Activities ◽

Zone Of Inhibition ◽

Microbiological Investigation ◽

Ethyl Acetate Extracts

The crude n-hexane and ethyl acetate extract of the stem bark of E. stricta were subjected to microbiological investigation and were found to be significantly inhibitory to microbial growth, with the average zone of inhibition 12–17 and 10–16 mm, respectively. In the cytotoxic observation, the n-hexane and ethyl acetate extracts were found to show LC50 of 2.1 and 0.316 mg/ml respectively. Key words: Erythrina stricta; Fabaceae; Antimicrobial activities; Cytotoxicity observation. DOI: http://dx.doi.org/10.3329/bjm.v27i2.9176 BJM 2010; 27(2): 65-66

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Antimicrobial Activities of Medicinal Plants Containing Phenolic Compounds

The Natural Products Journal ◽

10.2174/2210315510666191227150222 ◽

2020 ◽

Vol 10 (5) ◽

pp. 514-534

Author(s):

Tuğçe Dikpınar ◽

Sevda Süzgeç-Selçuk

Keyword(s):

Antimicrobial Activity ◽

Phenolic Compounds ◽

Medicinal Plants ◽

Antimicrobial Agents ◽

Inhibition Zone ◽

Relevant Information ◽

Antimicrobial Activities ◽

Molecular Structures ◽

Human Pathogens ◽

Antimicrobial Substances

Background: Medicinal plants have been used to treat diseases for centuries. They are important sources in terms of their pharmacological effects and also have many microbial agents. Recently, the development of drug resistance has begun to spread in human pathogens against used antibiotics and this has led to new research for novel antimicrobial substances from natural products containing plants. Objective: The purpose of this review is to determine the antimicrobial activity of pure phenolic compounds isolated from medicinal plants, to evaluate how molecular structures of these compounds affect the activity, and to provide to the readers a source for future studies on natural antimicrobial agents. Methods: Relevant information was gathered from scientific engines and databases (Google Scholar, Web of Science, Scifinder, Science Direct, Scopus, Wiley Online Library, PubMed, Taylor & Francis online) using different keywords. Antimicrobial activity research was selected especially on pure phenolic compounds. Results: Numerous phenolic compounds were isolated from plants/plant extracts and were shown to prevent the growth of bacteria and fungi and their Minimum Inhibition Concentration (MIC) and inhibition zone values were given in detail. Conclusion: This review revealed that phenolic compounds found in medicinal plants have different antimicrobial activity according to their changes in the molecular structure.

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Assessment of Antimicrobial Activity and total Polyphenol Content of Rosa multiflora and Rosa wichuraiana

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.10.4.15 ◽

2019 ◽

Vol 10 (04) ◽

pp. 646-650

Author(s):

Hyeusoo Kim ◽

Kyeong Won Yun

Keyword(s):

Antimicrobial Activity ◽

Ethyl Acetate ◽

Antimicrobial Agents ◽

Ethyl Acetate Fraction ◽

Rosa Multiflora ◽

Polyphenol Content ◽

Total Polyphenol ◽

Water Fraction ◽

Total Polyphenol Content ◽

Rosa Species

The fruit of Rosa multiflora has been used as traditional herbal medicine in Asian countries. The present investigation was undertaken to study the antimicrobial activity and total polyphenol content of hexane, ether, ethyl acetate, water fraction of methanol extract of fruit and flower from Rosa multiflora and Rosa wichuraiana. Antimicrobial activity of the mentioned fractions against 3 gram-positive and 4 gram-negative bacteria using disk diffussion method. The measurement of minimal inhibition concentration (MIC) showed that the ethyl acetate fraction of the two Rosa species is the most effective against the tested bacteria. The total polyphenol content of ethyl acetate fraction of the two Rosa species is higher than the other fractions. The results indicate the antimicrobial activity was related with the total polyphenol content and the fruit and flower of the two Rosa species can be considered as a natural source of antimicrobial agents.

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Synthesis of Novel 3(N,N-dialkylamino)alkyl/phenyl Substituted Thieno [2,3-d]pyrimidinones as H1-Anti-Histaminic and Antimicrobial Agents

Current Bioactive Compounds ◽

10.2174/1573407214666180226130957 ◽

2019 ◽

Vol 15 (1) ◽

pp. 63-70

Author(s):

Shiv Dev Singh ◽

Arvind Kumar ◽

Firoz Babar ◽

Neetu Sachan ◽

Arun Kumar Sharma

Keyword(s):

Antimicrobial Activity ◽

Potassium Hydroxide ◽

Antimicrobial Agents ◽

Pyrimidine Ring ◽

Antimicrobial Activities ◽

Screening Methods ◽

Chlorpheniramine Maleate ◽

In Vivo Screening

Background: Thienopyrimidines are the bioisoster of quinazoline and unlike quinazoline exist in three isomeric forms corresponding to the three possible types annulation of thiophene to the pyrimidine ring viz thieno[2,3-d] pyrimidine, thieno[3,2-d] pyrimidine and thieno[3,4-d]pyrimidine. Heterocyclic containing the thienopyrimidinone moiety exhibits various pronounced activities such as anti-hypertensive, analgesic and anti-inflammatory, antiviral, platelet aggregation inhibitory, antiprotozoal bronchodilatory, phosphodiesterase inhibitory, antihistaminic, antipsychotic and antimicrobial activity. Objective: Synthesis of novel 3(N,N-dialkylamino)alkyl/phenyl substituted thieno[2,3-d]pyrimidinones as H1-anti-histaminic and antimicrobial agents. Methods: A series of 3-[(N,N-dialkylamino)alkyl/phenyl]-2-(1H)thioxo-5,6,7,8-tetrahydrobenzo(b) thieno(2,3-d)pyrimidine-4(3H)-ones[4a-d], their oxo analogous [5a-d] and 3-[(N,N-dialkylamino)alkyl]- 2-chlorophenyl-5,6,7,8-tetrahydrobenzo(b)thieno(2,3-d)pyrimidine- 4 (3H)-ones[6a-d]derivative were synthesized from 2-amino-4,5,6,7-tetrahydrobenzo(b)thiophene-3-carboxylic acid by nucleophilic substitution of different N,N-dialkyl alkylene/phenylene diamines on activated 3-acylchloride moiety followed by cyclocondensation with carbon disulfide and ethanolic potassium hydroxide to get [4a-d] and in second reaction by condensation with 4-chlorobenzoyl chloride to get [6a-d] by single pot novel innovative route. The oxo analogous [5a-d] were prepared by treating derivatives [4a-d] with potassium permagnate in ethanolic KOH. The synthesized compound were evaluated for H1-antihistaminic and antimicrobial activities. Results: All synthesized compounds exhibited significant H1-antihistaminic activity by in vitro and in vivo screening methods and data were verified analytically and statistically. The compound 4a, 4b, 5a and 5b showed significant H1-antihistaminiic activity than the reference standard chlorpheniramine maleate. The compound 6d, 6c, 5c and 4c exhibited significant antimicrobial activity.

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Synergistic Antimicrobial Activities of Combinations of Vanillin and Essential Oils of Cinnamon Bark, Cinnamon Leaves and Cloves

Foods ◽

10.3390/foods10061406 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1406

Author(s):

Rita Cava-Roda ◽

Amaury Taboada-Rodríguez ◽

Antonio López-Gómez ◽

Ginés Benito Martínez-Hernández ◽

Fulgencio Marín-Iniesta

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Food Packaging ◽

Antimicrobial Effect ◽

Antimicrobial Activities ◽

Inhibitory Effects ◽

Cinnamon Bark ◽

E Coli ◽

Pure Compounds ◽

Main Component

Plant bioactive compounds have antimicrobial and antioxidant activities that allow them to be used as a substitute for synthetic chemical additives in both food and food packaging. To improve its sensory and bactericidal effects, its use in the form of effective combinations has emerged as an interesting possibility in the food industry. In this study, the antimicrobial activities of essential oils (EOs) of cinnamon bark, cinnamon leaves, and clove and the pure compounds vanillin, eugenol, and cinnamaldehyde were investigated individually and in combination against Listeria monocytogenes and Escherichia coli O157:H7. The possible interactions of combinations of pure compounds and EOs were performed by the two-dimensional checkerboard assay and isobologram methods. Vanillin exhibited the lowest antimicrobial activity (MIC of 3002 ppm against L. monocytogenes and 2795 ppm against E. coli O157:H7), while clove and cinnamon bark EOs exhibited the highest antimicrobial activity (402–404 against L. monocytogenes and 778–721 against E. coli O157:H7). For L. monocytogenes, pure compound eugenol, the main component of cinnamon leaves and clove, showed lower antimicrobial activity than EOs, which was attributed to the influence of the minor components of the EOs. The same was observed with cinnamaldehyde, the main component of cinnamon bark EO. The combinations of vanillin/clove EO and vanillin/cinnamon bark EO showed the most synergistic antimicrobial effect. The combination of the EOs of cinnamon bark/clove and cinnamon bark/cinnamon leaves showed additive effect against L. monocytogenes but indifferent effect against E. coli O157:H7. For L. monocytogenes, the best inhibitory effects were achieved by cinnamon bark EO (85 ppm)/vanillin (910 ppm) and clove EO (121 ppm)/vanillin (691 ppm) combinations. For E. coli, the inhibitory effects of clove EO (104 ppm)/vanillin (1006 ppm) and cinnamon leaves EO (118 ppm)/vanillin (979 ppm) combinations were noteworthy. Some of the tested combinations increased the antimicrobial effect and would allow the effective doses to be reduced, thereby offering possible new applications for food and active food packaging.

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Biological and Physico-Chemical Characteristics of Arginine-Rich Peptide Gemini Surfactants with Lysine and Cystine Spacers

International Journal of Molecular Sciences ◽

10.3390/ijms22073299 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3299

Author(s):

Damian Neubauer ◽

Maciej Jaśkiewicz ◽

Marta Bauer ◽

Agata Olejniczak-Kęder ◽

Emilia Sikorska ◽

...

Keyword(s):

Antimicrobial Activity ◽

Amino Acid ◽

Cationic Surfactants ◽

Antimicrobial Agents ◽

Gemini Surfactants ◽

Antimicrobial Activities ◽

Critical Aggregation Concentration ◽

Cytotoxic Activities ◽

Candida Sp ◽

Enhanced Selectivity

Ultrashort cationic lipopeptides (USCLs) and gemini cationic surfactants are classes of potent antimicrobials. Our recent study has shown that the branching and shortening of the fatty acids chains with the simultaneous addition of a hydrophobic N-terminal amino acid in USCLs result in compounds with enhanced selectivity. Here, this approach was introduced into arginine-rich gemini cationic surfactants. L-cystine diamide and L-lysine amide linkers were used as spacers. Antimicrobial activity against planktonic and biofilm cultures of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.) strains and Candida sp. as well as hemolytic and cytotoxic activities were examined. Moreover, antimicrobial activity in the presence of human serum and the ability to form micelles were evaluated. Membrane permeabilization study, serum stability assay, and molecular dynamics were performed. Generally, critical aggregation concentration was linearly correlated with hydrophobicity. Gemini surfactants were more active than the parent USCLs, and they turned out to be selective antimicrobial agents with relatively low hemolytic and cytotoxic activities. Geminis with the L-cystine diamide spacer seem to be less cytotoxic than their L-lysine amide counterparts, but they exhibited lower antibiofilm and antimicrobial activities in serum. In some cases, geminis with branched fatty acid chains and N-terminal hydrophobic amino acid resides exhibited enhanced selectivity to pathogens over human cells.

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