scholarly journals Combinatorial Assembly and Optimisation of Designer Cellulosomes - A Galactomannan Case Study

2021 ◽  
Author(s):  
Julie Vanderstraeten ◽  
Maria João Maurício da Fonseca ◽  
Philippe De Groote ◽  
Dennis Grimon ◽  
Hans Gerstmans ◽  
...  
Keyword(s):  
Dna Assembly ◽  
Assembly Method ◽  
Rapid Construction ◽  
Starting Point ◽  
Combinatorial Assembly ◽  
Designer Cellulosome ◽  
Enzyme Complexes ◽  
Enzyme Ratio ◽  
Combinatorial Arrangements

Abstract Background: Designer cellulosomes are self-assembled chimeric enzyme complexes that can be used to improve lignocellulosic biomass degradation. They are composed of a synthetic multimodular backbone protein, termed the scaffoldin, and a range of different chimeric docking enzymes that degrade polysaccharides. Over the years, several functional designer cellulosomes have been constructed. Since many parameters influence the efficiency of these multi-enzyme complexes, there is a need to optimise designer cellulosome architecture by testing combinatorial arrangements of docking enzyme and scaffoldin variants. However, the modular cloning procedures are tedious and cumbersome. Results: VersaTile is a combinatorial DNA assembly method, allowing the rapid construction and thus comparison of a range of modular proteins. Here, we present the extension of the VersaTile platform to facilitate the construction of designer cellulosomes. We have constructed a tile repository, composed of dockerins, cohesins, linkers, tags and enzymatically active modules. The developed toolbox allows us to efficiently create and optimise designer cellulosomes at an unprecedented speed. As a proof of concept, a trivalent designer cellulosome able to degrade the specific hemicellulose substrate, galactomannan, was constructed and optimised. The main factors influencing cellulosome efficiency were found to be the selected dockerins and linkers and the docking enzyme ratio on the scaffoldin. The optimised designer cellulosome was able to hydrolyse the galactomannan polysaccharide and release mannose and galactose monomers. Conclusion: We have eliminated one of the main technical hurdles in the designer cellulosome field and anticipate the VersaTile platform to be a starting point in the development of more elaborate multi-enzyme complexes.

scholarly journals Start-Stop Assembly: a functionally scarless DNA assembly system optimised for metabolic engineering

2018 ◽  
Author(s):  
George M. Taylor ◽  
Paweł M. Mordaka ◽  
John T. Heap
Keyword(s):  
Metabolic Engineering ◽  
Design Space ◽  
Combinatorial Libraries ◽  
Dna Assembly ◽  
Destination Vector ◽  
E Coli ◽  
Assembly Method ◽  
Wide Range ◽  
Combinatorial Assembly ◽  
Sequence Boundaries

ABSTRACTDNA assembly allows individual DNA constructs or designed mixtures to be assembled quickly and reliably. Most methods are either: (i) Modular, easily scalable and suitable for combinatorial assembly, but leave undesirable ‘scar’ sequences; or (ii) bespoke (non-modular), scarless but less suitable for construction of combinatorial libraries. Both have limitations for metabolic engineering. To overcome this trade-off we devised Start-Stop Assembly, a multi-part, modular DNA assembly method which is both functionally scarless and suitable for combinatorial assembly. Crucially, 3 bp overhangs corresponding to start and stop codons are used to assemble coding sequences into expression units, avoiding scars at sensitive coding sequence boundaries. Building on this concept, a complete DNA assembly framework was designed and implemented, allowing assembly of up to 15 genes from up to 60 parts (or mixtures); monocistronic, operon-based or hybrid configurations; and a new streamlined assembly hierarchy minimising the number of vectors. Only one destination vector is required per organism, reflecting our optimisation of the system for metabolic engineering in diverse organisms. Metabolic engineering using Start-Stop Assembly was demonstrated by combinatorial assembly of carotenoid pathways inE. coliresulting in a wide range of carotenoid production and colony size phenotypes indicating the intended exploration of design space.GRAPHICAL ABSTRACT

Ein rechtsfreier Raum? Die legale Situation auf den Färöern im Spiegel der ‚Færeyinga saga‘

2020 ◽  
Vol 25 (1) ◽  
pp. 30-45
Author(s):  
Andreas Schmidt
Keyword(s):  
Cultural Norms ◽  
Old Icelandic ◽  
Power Politics ◽  
Starting Point ◽  
Narrative Literature ◽  
The Law

AbstractThe chapter argues for a more nuanced and empirically based understanding of the discourse on law and socio-cultural norms in Old Icelandic literature on the grounds of a narratological reading of ‘Færeyinga saga’ as a case study. It has often been claimed that Icelandic sources express an ideal of freedom based on communality as guaranteed by the law. By contrast, ‘Færeyinga saga’ represents a cynical discourse on power politics that renders law as an invariable concept obsolete and works solely on the principle that ‘might is right’. This cynicism, however, is presented in a form that leaves the narrative open to interpretation, showing that regardless of its possible dating, narrative literature can serve as a starting point for social discussion. Consequently, the discourse on law in medieval Iceland must be perceived as more polyphonic than has been allowed for by previous unifying readings in scholarship.

‘More Product, Less Process’ (MPLP): An early printed book project

2021 ◽  
pp. 096100062110267
Author(s):  
Karen Attar
Keyword(s):  
18Th Century ◽  
The United States ◽  
Global Changes ◽  
Main Research ◽  
Advantages And Disadvantages ◽  
Main Research Interest ◽  
Printed Books ◽  
Starting Point ◽  
19Th And 20Th Century

This article addresses the challenge to make printed hidden collections known quickly without sacrificing ultimate quality. It takes as its starting point the archival mantra ‘More product, less process’ and explores its application to printed books, mindful of projects in the United States to catalogue 19th- and 20th-century printed books quickly and cheaply with the help of OCLC. A problem is lack of time or managerial inclination ever to return to ‘quick and dirty’ imports. This article is a case study concerning a collection of 18th-century English imprints, the Graveley Parish Library, at Senate House Library, University of London. Faced with the need to provide metadata as quickly as possible for digitisation purposes, Senate House Library decided, in contrast to its normal treatment of early printed books, to download records from the English Short Title Catalogue and amend them only very minimally before releasing them for public view, and to do this work from catalogue cards rather than the books themselves. The article describes the Graveley Parish collection, the project method’s rationale, and the advantages and disadvantages of sourcing the English Short Title Catalogue for metadata. It discusses the drawbacks of retrospective conversion (cataloguing from cards, not books): insufficient detail in some cases to identify the relevant book, and ignorance of the copy-specific elements of books which can constitute the main research interest. The method is compared against cataloguing similar books from photocopies of title pages, and retrospective conversion using English Short Title Catalogue is compared against retrospective conversion of early printed Continental books from cards using Library Hub Discover or OCLC. The control groups show our method’s effectiveness. The project succeeded by producing records fast that fulfilled their immediate purpose and simultaneously would obviously require revisiting. The uniform nature of the collection enabled the saving of time through global changes.

scholarly journals The DWQ-EMR Embedded Tool to Enhance the Family Physician-Caregiver Connection: A Pilot Case Study

Geriatrics ◽  
2021 ◽  
Vol 6 (1) ◽  
pp. 29
Author(s):  
Kristina Marie Kokorelias ◽  
Einat Danieli ◽  
Sheila Dunn ◽  
Sid Feldman ◽  
David Patrick Ryan ◽  
...  
Keyword(s):  
Family Caregivers ◽  
Best Practice ◽  
Data Gathering ◽  
Family Physicians ◽  
Efficient Manner ◽  
Efficient Access ◽  
Increased Risk ◽  
Starting Point ◽  
To Receive

The number of family caregivers to individuals with dementia is increasing. Family physicians are often the first point of access to the health care system for individuals with dementia and their caregivers. Caregivers are at an increased risk of developing negative physical, cognitive and affective health problems themselves. Caregivers also describe having unmet needs to help them sustain care in the community. Family physicians are in a unique position to help support caregivers and individuals with dementia, but often struggle with keeping up with best practice dementia service knowledge. The Dementia Wellness Questionnaire was designed to serve as a starting point for discussions between caregivers and family physicians by empowering caregivers to communicate their needs and concerns and to enhance family physicians’ access to specific dementia support information. The DWQ aims to alert physicians of caregiver and patient needs. This pilot study aimed to explore the experiences of physicians and caregivers of people using the Questionnaire in two family medicine clinics in Ontario, Canada. Interviews with physicians and caregivers collected data on their experiences using the DWQ following a 10-month data gathering period. Data was analyzed using content analysis. Results indicated that family physicians may have an improved efficacy in managing dementia by having dementia care case specific guidelines integrated within electronic medical records. By having time-efficient access to tailored supports, family physicians can better address the needs of the caregiver–patient dyad and help support family caregivers in their caregiving role. Caregivers expressed that the Questionnaire helped them remember concerns to bring up with physicians, in order to receive help in a more efficient manner.

Deepfaking Keanu: YouTube deepfakes, platform visual effects, and the complexity of reception

2021 ◽  
pp. 135485652110304
Author(s):  
Lisa Bode
Keyword(s):  
Film Studies ◽  
Media Theory ◽  
Digital Rhetoric ◽  
Visual Effects ◽  
After Effects ◽  
Starting Point ◽  
Media Creation ◽  
Media Practices

On July 14, 2019, a 3-minute 36-second video titled “Keanu Reeves Stops A ROBBERY!” was released on YouTube visual effects (VFX) channel, Corridor. The video’s click-bait title ensured it was quickly shared by users across platforms such as Facebook, Twitter, and Reddit. Comments on the video suggest that the vast majority of viewers categorised it as fiction. What seemed less universally recognised, though, was that the performer in the clip was not Keanu Reeves himself. It was voice actor and stuntman Reuben Langdon, and his face was digitally replaced with that of Reeves, through the use of an AI generated deepfake, an open access application, Faceswap, and compositing in Adobe After Effects. This article uses Corridor’s deepfake Keanu video (hereafter shorted to CDFK) as a case study which allows the fleshing out of an, as yet, under-researched area of deepfakes: the role of framing contexts in shaping how viewers evaluate, categorise, make sense of and discuss these images. This research draws on visual effects scholarship, celebrity studies, cognitive film studies, social media theory, digital rhetoric, and discourse analysis. It is intended to serve as a starting point of a larger study that will eventually map types of online manipulated media creation on a continuum from the professional to the vernacular, across different platforms, and attending to their aesthetic, ethical, cultural and reception dimensions. The focus on context (platform, creator channel, and comments) also reveals the emergence of an industrial and aesthetic category of visual effects, which I call here “platform VFX,” a key term that provides us with more nuanced frames for illuminating and analysing a range of manipulated media practices as VFX software becomes ever more accessible and lends itself to more vernacular uses, such as we see with various face swap apps

scholarly journals Implications of Lesson Study for Tertiary-Level EFL Teachers’ Professional Development: A Case Study From Turkey

SAGE Open ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 215824402110237
Author(s):  
İlknur Bayram ◽  
Fatma Bıkmaz
Keyword(s):  
Professional Development ◽  
Lesson Study ◽  
English Language ◽  
Research Question ◽  
Teaching Style ◽  
Language Teachers ◽  
Student Thinking ◽  
Pedagogical Content ◽  
Starting Point

This qualitative case study carried out at a Turkish university with four English language teachers aims to explore what teachers experience in the planning, implementation, analysis, and reporting phases of the lessons study process and what the implications of lesson study for teacher professional development can be. Data in this four-month study were gathered through observations, interviews, whole group discussions, and reflective reports. Findings revealed that lesson study had potential challenges and benefits for the professional development of teachers. The model poses challenges in finding a topic and research question, determining the lesson design and teaching style, making student thinking observable and analyzing qualitative data. On the other hand, it benefited teachers in terms of increasing their pedagogical content knowledge, reflectivity, research skills, collaboration, and collegiality. This study suggests that lesson study might be a good starting point for institutions wishing to adopt a more teacher-led, inquiry-driven and collaborative perspective for professional development.

Od politycznej współpracy do gospodarczej konkurencji – przemysł obronny krajów Europy Środkowo-Wschodniej po upadku ZSRR

2021 ◽  
Vol 19 (2) ◽  
pp. 81-98
Author(s):  
Mieczysław Szlachta ◽  
Andrzej Ciupiński
Keyword(s):  
Cold War ◽  
Economic Recession ◽  
Defense Industry ◽  
Economic Transformation ◽  
The Political ◽  
Economic Sectors ◽  
Central Eastern Europe ◽  
Starting Point ◽  
The Cold War

The paper presents the scope and scale of transformation of the defense industries of Central Eastern Europe (CEE) countries after the end of the Cold War and the collapse of the USSR. The starting point is the role and position of the armaments economy sector (armaments economy environment), embedded in the realities of the centrally planned economy, and its submission to the politics of the USSR. The turn of the centuries was a period of political and economic transformation conducted during the conditions of a deep economic recession. The defense industry was one of the economic sectors most affected by the crisis. The economic and defense policy of CEE countries was aimed at preserving the capabilities of the armaments sector. Restructuring activities initiated and forced by the change of the political and economic environment have already brought noticeable effects, even though the process has not yet been completed. Defense industry enterprises have become entities operating on the same terms and conditions as other companies on the competitive market. The method of comparative analysis and a case study supplemented with elements of descriptive statistics were used to evaluate the course of the processes. The study has been focused on the analysis of the course of the changes and examination of effects of the analyzed phenomena for the economy and defense of the CEE countries, taking into account primarily their scale and scope.

Mapping OWL-S Process Model to Timed Automata

2018 ◽  
Vol 11 (1) ◽  
pp. 29-48
Author(s):  
Amel Boumaza ◽  
Ramdane Maamri
Keyword(s):  
Web Services ◽  
Process Model ◽  
Timed Automata ◽  
Automatic Verification ◽  
Covering Problems ◽  
Field Verification ◽  
Starting Point ◽  
Quantitative Properties ◽  
Time Systems

The conversion of web services to semantic web comes the opportunity to automate various tasks. OWL-S plays a key role in describing web services behaviour. While ontology-based semantics given to OWL-S is structural rather than behaviourally oriented, we cannot automate an essential task in this field, verification. In this article, the mapping of OWL-S process model to Timed automata is investigated, which is a suitable formalism for real time systems modeling and automatic verification. Hence, this has led to not only enabling automatic verification but also covering problems related to automated verification of temporal quantitative properties as bounded liveness property. As a starting point, the OWL-S and sub entry of time ontologies for describing the timed behaviour of services has been chosen. A defined set of mapping rules is used to automatically encode control constructs defined in OWL-S and temporal information into timed automata. Also, it is shown how a Uppaal checker is used to check required properties formulated in TCTL. Finally, an EClinic case study is used to illustrate the technique.

Intellectual capital in family firms: human capital identification and measurement

2015 ◽  
Vol 16 (1) ◽  
pp. 199-223 ◽  
Author(s):  
Enrique Claver-Cortés ◽  
Patrocinio Carmen Zaragoza-Sáez ◽  
Hipólito Molina-Manchón ◽  
Mercedes Úbeda-García
Keyword(s):  
Human Capital ◽  
Intellectual Capital ◽  
Family Firms ◽  
Balance Sheet ◽  
Research Approach ◽  
Competitive Advantages ◽  
Content Type ◽  
Starting Point ◽  
Practical Implications

Purpose – Based on the literature devoted to family firms and the intellectual capital-based view of the firm, the purpose of this paper is not only to identify the most important human capital intangibles owned by family firms but also to show a number of indicators that can help measure them. Design/methodology/approach – A qualitative case-study-based research approach was adopted taking as reference: 25 family firms belonging to different sectors; previous works existing in the literature; and the intellectus model. Findings – The present study identifies ten intangibles associated with the human capital of family firms and shows 60 indicators that can be used to measure them. It additionally provides empirical evidence and gives examples of these intangibles through the analysis of 25 international family firms. Research limitations/implications – The difficulty in collecting all the human capital intangibles of family firms; the problems associated with the creation of accurate indicators; and those specific to the research methodology adopted. Practical implications – Identifying the human capital intangibles of family firms and their indicators can help managers become aware of their importance, and this will consequently help them improve their management. This could be an interesting starting point to value these intangibles in the balance sheet as well as to draw comparisons between family and non-family organisations. Originality/value – The framework provided by family firms sheds light on several intangibles specific to these firms – precisely for their condition as “family” firms. Those intangibles – human capital intangibles being especially highlighted in this study – provide the basis for the achievement of competitive advantages.

A Computational Product Model for Conceptual Design Using SysML

2009 ◽  
Author(s):  
Stefan Wo¨lkl ◽  
Kristina Shea
Keyword(s):  
Product Development ◽  
Conceptual Design ◽  
Mechanical Design ◽  
System Development ◽  
Concept Design ◽  
Product Modeling ◽  
Product Model ◽  
Mechatronic Systems ◽  
Starting Point

The importance of the concept development phase in product development is contradictory to the level and amount of current computer-based support for it, especially with regards to mechanical design. Paper-based methods for conceptual design offer a far greater level of maturity and familiarity than current computational methods. Engineers usually work with software designed to address only a single stage of the concept design phase, such as requirements management tools. Integration with software covering other stages, e.g. functional modeling, is generally poor. Using the requirements for concept models outlined in the VDI 2221 guideline for systematic product development as a starting point, the authors propose an integrated product model constructed using the Systems Modeling Language (SysML) that moves beyond geometry to integrate all necessary aspects for conceptual design. These include requirements, functions and function structures, working principles and their structures as well as physical effects. In order to explore the applicability of SysML for mechanical design, a case study on the design of a passenger car’s luggage compartment cover is presented. The case study shows that many different SysML diagram types are suitable for formal modeling in mechanical concept design, though they were originally defined for software and control system development. It is then proposed that the creation and use of libraries defining generic as well as more complicated templates raises efficiency in modeling. The use of diagrams and their semantics for conceptual modeling make SysML a strong candidate for integrated product modeling of mechanical as well as mechatronic systems.

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