scholarly journals Sustained of mir-499-5p delivery from injection alters the muscle metabolomic profiles in broiler chicken

2020 ◽  
Author(s):  
Zhixiong Li ◽  
Yaqiu Lin ◽  
Mao Yuan ◽  
Xiaosong Jiang ◽  
Chaowu Yang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Treatment Group ◽  
Broiler Chicken ◽  
Inositol Phosphate ◽  
Control Group ◽  
Broiler Chicks ◽  
Galactose Metabolism ◽  
Metabolic Pathway Analysis ◽  
Critical Pathway ◽  
Mannose Metabolism

Abstract Background Myogenic miRNAs (myomiRs) which dramatically increased during myogenesis have been shown to play critical roles in many aspects of muscle function. As a myomiR, miR-499-5p, has been identified to be highly expressed in cardiac and skeletal muscle. The study focused on the effects of miR-499-5p on muscle metabolism in broiler chicken. Methods In the current study, we assigned 16 broiler chicks to control group and treatment group and then monitored the effects using metabolomics. Chicks were fed basal diets without or with miR-499-5p delivery. Muscle samples were collected and analyzed by ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). Results Our findings revealed that miR-499-5p injection altered the concentrations of a variety of metabolites in the muscle. Thereinto, a total of 46 metabolites were identified at higher (P < 0.05) concentrations and 30 metabolites were identified at lower (P < 0.05) concentrations in the treatment group as compared with the control group. These metabolites were primarily involved with the regulation of lipid and carbohydrate metabolism. Further metabolic pathway analysis revealed that fructose and mannose metabolism, galactose metabolism, inositol phosphate metabolism and terpenoid backbone biosynthesis were the most important and critical pathway which may partially interpret the effects of miR-499-5p. Conclusions To our knowledge, this research is the first report of metabolic signatures and related metabolic pathways in the skeletal muscle for miR-499-5p injection and provide new insight into the effect of miRNA on growth performance.

scholarly journals Effects of different stocking densities on the trachea barrier function and its plasma metabolic profile of finishing broilers

2020 ◽  
Author(s):  
Yuanyuan Wang ◽  
Dianchun Wang ◽  
Jiangshui Wang ◽  
Kaixuan Li ◽  
Chianning Heng ◽  
...  
Keyword(s):  
Treatment Group ◽  
Metabolic Profile ◽  
Stocking Density ◽  
Pentose Phosphate ◽  
Control Group ◽  
Metabolic Pathway Analysis ◽  
Critical Pathway ◽  
Diagnostic Biomarkers ◽  
Significant Difference ◽  
Lower Expression

Abstract Background The experiment was conducted to evaluate the effects of different stocking densities on the trachea barrier and its plasma metabolic profile of finishing broilers.Methods One thousand four hundred and forty 22-day-old Lingnan Yellow feathered broilers were randomly allotted to five different stocking density groups (8, 10, 12, 14, and 16 birds per m2). Each group consisted of three replicates.Results A markedly increased in IL-1β and IL-10 concentrations were observed in 16 birds per m2 treatment group than the groups of 8 and 10 birds per m2 (P < 0.05), but there was no significant difference in IL-4 content among five treatments (P > 0.05). 16 birds per m2 (high stocking density group, HSD) treatment group significantly increased the trachea mucosa thickness than 10 birds per m2 (control group, CSD). HSD group exhibited lower expression of claudin1 and higher muc2 and caspase3 than those in the CSD group. In addition, a total of 10 metabolites were identified at higher (P < 0.05) concentrations, while 7 metabolites were identified at lower (P < 0.05) concentrations in the HSD treatment as compared with the CSD group. Most of these potentially diagnostic biomarkers were involved in substance synthesis and energy metabolism. Further metabolic pathway analysis revealed that pentose and glucuronate interconversions along with pentose phosphate pathway were the most relevant and critical pathway identified from these two groups. The activated pathway may partially interpret the metabolic level to alleviate the adverse effects of stress which caused by high-stocking density in broilers.Conclusions In summary, this study can improve our understanding of the harmful effects of high-stocking density on the trachea barrier and identify two metabolic pathways that may be associated with high-stocking density-induced metabolic disorders in broilers.

scholarly journals Probiotic feeding effect of Bacillus subtilis on broilers chicks’ microflora, TLRs and interleukin gene expression

2021 ◽  
Author(s):  
Zaigui Wang ◽  
Ru Yang ◽  
Salman Khan ◽  
Chunjie wei ◽  
Hu Qian ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Protease Activity ◽  
Broiler Chicken ◽  
Amylase Activity ◽  
Digestive Enzyme ◽  
Digestive Enzyme Activity ◽  
Control Group ◽  
Broiler Chicks ◽  
Treatment Groups

Abstract The broiler chicks provide the major portion of daily food items. The current study aimed to investigate the effects of Bacillus subtilis (BS) on broiler chicken gut microflora diversity, digestive enzyme activity and expression of Toll-like receptors (TLRs). A total of 240 crossbred broiler chickens were randomly allocated into 4 groups with 3 replicates. The groups were named as control group (basal diet, BD), group Ⅰ (BD with 300 g/d BS,1.08×107 CFU/kg), group Ⅱ (BD with 600 g/d BS, 2.16×107 CFU/kg), and group Ⅲ (BD with 900 g/d BS, 3.24×107 CFU/kg). Samples were collected at the 21st day. The difference of α diversity and β diversity between control group and treatment groups were not significant, but the abundance of some microorganisms in the treatment groups were improved, and the core microorganisms were different between groups. In comparison of control group, the protease activity in ileum of experimental groups was significantly increased (p < 0.05). Protease activity in ileum of group Ⅱ and Ⅲ was improved by 22.59% (p < 0.01) and 14.49% (p < 0.05). The amylase activity in ileum of treatment groups were also significantly increased (p < 0.05). Amylase activity of group Ⅱ was increased by 41.85% (p < 0.01) in comparison to control, group Ⅰ and Ⅲ. Expression of TLR1A and TLR7 in the jejunum and caecum of treatment groups were significantly up-regulated while their expression in ileum was decreased. Similarly, the expression of TLR1B gene was significantly improved in the ileum, and down regulated in the cecum. Transcription levels of TLR2A and MyD88 in jejunum, liver, spleen and kidney were significantly increased, but their expression was significantly lower in the ileum and cecum. The TLR2B, TLR3, TLR4, TLR15, TLR21 expression were also significantly modulated in various organ of broiler chicken. The most important inflammatory factor such as IL-1β expressions in spleen and kidney were significantly increased in the current data. Bacillus subtilis could not regulate the caecal microflora diversity, but improved amount of some gut probiotics such as lactobacillus, the digestive enzyme activity, regulate some immunogenic expressions and enhance the immune capacity of animal. We hope that the impact of this data will explore the diseases control and feeding quality of broiler chicks at industrial level.

Hepatoprotective Role of AV/LCP/18 In CCl4 Induced FLKS Syndrome in Commercial Broilerst

2016 ◽  
Vol 12 (2) ◽  
Author(s):  
P. M. Sonkusale ◽  
Pratik N. Inglepatil ◽  
Siddharth V. Jamadade
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Protein Profile ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Broiler Chicks ◽  
Mean Values ◽  
Group B

A total of 75 day old broiler chicks were divided into three equal groups. Group A served as negative control. Group B (positive control) and C (treatment group) were intoxicated with oral administration of CCl4 @ 1 ml/kg body weight after every 3rd day during 15-28 days of age. Group C was given AV/LCP/18 @ 0.10 ml / chick / day in drinking water during 29th-42nd day of age. At the end of experiment, significantly (P is less than 0.01) higher mean body weight and lower FCR was observed in treatment group C as compared to positive control group B. Similarly, significantly (P is less than 0.05) decreased levels of SGOT, SGPT, cholesterol, triglycerides, creatinine and higher mean values of serum protein profile along with mild degenerative changes in liver and kidney of broilers in group C were observed as compared to group B indicating therapeutic efficacy of AV/LCP/18 in ameliorating the toxic effects or FLKS induced by CCl4 in commercial broilers

EFFECT OF SUPPLEMENTATION OF DIFFERENT LEVELS OF DRIED TAMARIND PULP ON MEAT CHOLESTEROL, CARCASS YIELD, DRESSING PERCENTAGE AND ABDOMINAL FAT OF BROILER CHICKEN

2016 ◽  
Vol 12 (1) ◽  
Author(s):  
P.V. Patil ◽  
G.B. Shinde ◽  
A.B. Kanduri ◽  
P.H. Pawar
Keyword(s):  
Broiler Chicken ◽  
Control Group ◽  
Broiler Chicks ◽  
Carcass Yield ◽  
Treatment Groups ◽  
Group I ◽  
Commercial Broiler ◽  
Dressing Percentage ◽  
Different Levels ◽  
Control Group Group

The present study was conducted to investigate the effect of supplementation of different levels of dried Tamarind pulp on meat cholesterol, carcass yield and dressing percentage of broiler chicken. 300 healthy day old commercial broiler chicks of Vencob’ strain were selected and divided into five groups of 60 chicks in each group, three replicates of 20 birds in each group. The chicks of control group (Group I) were fed with standard broiler diet without dried Tamarind pulp, whereas dried Tamarind pulp was supplemented @250 gm, 500 gm, 1000 gm and 1500 gm per 100 kg of broiler ration respectively to groups -II, III, IV and V.The meat cholesterol was found to be significantly lower in treatments groups than in control group. However, carcass yield and dressing percentage of treatment groups were significantly higher than control Group.The carcass yield and dressing percentage were increased and meat cholesterol level was decreased on supplementation of dried Tamarind pulp in broiler rations.

Effect of Thermal Stress on Serum Biochemical and Haematological Parameters in Broiler Chicken

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
A. K. Chaturvedani ◽  
Sudhir Kumar Jaiswal ◽  
Meesam Raza
Keyword(s):  
Thermal Stress ◽  
Treatment Group ◽  
Biochemical Parameters ◽  
Broiler Chicken ◽  
Room Temperature ◽  
Haematological Parameters ◽  
Control Group ◽  
Serum Biochemical Parameters ◽  
Serum Biochemical

The present study was conducted to assess the effects of thermal stress on the serum biochemical and haematological parameters in broiler chicken. Forty CARIBRO- Vishal broiler chicken were divided into two groups with twenty birds each. The control group was kept at room temperature and treatment group was exposed to thermal stress under 40±1 °C for 4 hours at 35 day of age. The exposure of thermal stress significantly increased (P less than 0.05) the value of serum biochemical parameters (glucose, cholesterol and triglyceride), heterophills, H/L ratios and basophills, however the value of total leukocyte, haemoglobin, haematocrit value, and monocytes decreased significantly (P less than 0.05).

Evaluation of aspirin (ASA) in broiler chicken during hot dry summer using zoo technical, molecular and physio-biochemical tools

2016 ◽  
Author(s):  
J.J. Rokade ◽  
S.K. Bhanja ◽  
A.S. Shinde ◽  
Sajjad, Darshana ◽  
B. Bhaisare ◽  
...  
Keyword(s):  
Broiler Chicken ◽  
Relative Yield ◽  
Live Weight ◽  
Dietary Treatment ◽  
Control Group ◽  
Broiler Chicks ◽  
Standard Diet ◽  
Treatment Groups ◽  
Improved Performance ◽  
Heat Stressor

The present study was undertaken to evaluate the efficacy of aspirin as anti-heat stressor. Broiler chicks were reared on a standard diet up to 14 days of age. Thereafter, the chicks were randomly distributed into three dietary treatment groups viz., T1 (Control group: Standard diet), T2 (Standard diet with aspirin@250 mg/kg) and T3 (Standard diet with aspirin@500 mg/kg). Each treatment was having five replicates of eight birds per replicate. Experiment was carried out during hot-dry (April-May, 30.0±0.70 to 37.0±1.40C, Rh, %: 58.05±1.32 to 70.11±0.82) summer. Feed intake and live weight gain was comparable among the treatments but FCR, protein and energy efficiency improved significantly (P less than 0.001) on aspirin supplementation. The relative yield of thymus and bursa at 4th and 6th week of age improved significantly (P less than 0.05). The percentage of protein, aspartate transaminase (AST) and alanine transaminase (ALT) increased significantly (P less than 0.001) due to aspirin supplementation at 4th as well as 6th week of age. While H:L ratio, serum corticosteron and serum cholesterol (only at 42nd day of age) decreased significantly (P less than 0.001) in aspirin supplemented groups at 28th as well as 42nd day of age. Supplementation of aspirin at 500 mg/kg reduced relative expression of HSP70 in jejunum tissues during 28th or 42nd day of age. It can be concluded that aspirin supplementation 500 mg/kg in diet of heat stressed broilers improved performance as well as welfare..

scholarly journals Effects of indigenous medicinal plant Tulsi (Ocimum sanctum) leaves extract as a growth promoter in broiler

2015 ◽  
Vol 2 (1) ◽  
pp. 97-102
Author(s):  
Firoj Alom ◽  
Mahbub Mostofa ◽  
M Nurul Alam ◽  
M Golam Sorwar ◽  
Jashim Uddin ◽  
...  
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Body Weight Gain ◽  
Hematological Parameters ◽  
Vaccination Schedule ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Live Body Weight

The study was conducted to determine the efficacy of Tulsi (Ocimum sanctum ) leaves extract as a growth promoter in broiler. Thirty (30) day-old broiler chicks were purchased from Kazi hatchery and after seven days of acclimatization in the poultry shed of Pharmacology department randomly divided into two groups I0 and I1. No vaccination schedule was practiced and no antibiotics were added in rations. Group was supplemented with Tulsi (Ocimum sanctum) leaves extract @ 2ml/litre in drinking water. Weekly observations were recorded for live body weight gain upto 5th weeks and blood test was performed at 17th and 35th day’s age of broiler to observe the hematological changes between control (Group) and treatment (Group) group. The treatment group (Group A) recorded statistically non- significant for live body weight at 1st and 2nd weeks than that of control group (Group B) but found statistically significant at 3rd (p<0.01), 4th (P<0.05) and 5th (P<0.01) weeks of age and the Hematological parameters (TEC, PCV, Hb and ESR) showed statistically significant (p<0.01) difference as compared to control group.Res. Agric., Livest. Fish.2(1): 97-102, April 2015

scholarly journals Effect of garlic extract on growth performances and hematological parameters of broilers

2017 ◽  
Vol 3 (3) ◽  
pp. 317-322
Author(s):  
Md Shafiqul Islam Khan ◽  
Md Shoaib Prodhan ◽  
Md Shahidul Islam ◽  
Md Nazmul Hasan ◽  
Md Shajadul Islam
Keyword(s):  
Weight Gain ◽  
Treatment Group ◽  
Body Weight Gain ◽  
Hematological Parameters ◽  
Live Weight ◽  
Garlic Extract ◽  
Control Group ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Group B

This experiment was conducted to evaluate the efficacy of garlic (Allium sativum) extracts supplementation in drinking water as a growth promoter of broiler chicken. A total of 60 one-day-old male Lohman meat broiler chicks were distributed over two groups of 30 chicks with three replicates of 10 birds. Group B was supplemented with garlic extract from water and Group A kept as a control. Weekly observations were recorded for live body weight gain up to 4th weeks and hematological tests were performed at 30th days. Live weight and weight gain of the treatment group were significantly (p<0.05) higher than that of the control group. Significant (p<0.05) higher values also observed in terms of weight of heart, liver, spleen, and pancreas for the treatment group. The value of TEC and PVC of treatment group showed significant (p<0.05) differences. The results suggest that better growth performance could be achieved in broilers with feed supplemented with garlic extract.Asian J. Med. Biol. Res. September 2017, 3(3): 317-322

scholarly journals (−)-Epicatechin-Enriched Extract from Camellia sinensis Improves Regulation of Muscle Mass and Function: Results from a Randomized Controlled Trial

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1026
Author(s):  
Hyeyeong Seo ◽  
Seok-Hee Lee ◽  
Yooheon Park ◽  
Hee-Seok Lee ◽  
Jeong Sup Hong ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Treatment Group ◽  
Muscle Mass ◽  
Green Tea ◽  
Green Tea Extract ◽  
Control Group ◽  
Therapeutic Benefits ◽  
Age Related ◽  
Tea Extract ◽  
And Function

Loss of skeletal muscle mass and function with age represents an important source of frailty and functional decline in the elderly. Antioxidants from botanical extracts have been shown to enhance the development, mass, and strength of skeletal muscle by influencing age-related cellular and molecular processes. Tannase-treated green tea extract contains high levels of the antioxidants (−)-epicatechin (EC) and gallic acid that may have therapeutic benefits for age-related muscle decline. The aim of this study was to investigate the effect of tannase-treated green tea extract on various muscle-related parameters, without concomitant exercise, in a single-center, randomized, double-blind, placebo-controlled study. Administration of tannase-treated green tea extract (600 mg/day) for 12 weeks significantly increased isokinetic flexor muscle and handgrip strength in the treatment group compared with those in the placebo (control) group. In addition, the control group showed a significant decrease in arm muscle mass after 12 weeks, whereas no significant change was observed in the treatment group. Blood serum levels of follistatin, myostatin, high-sensitivity C-reactive protein (hs-CRP), interleukin (IL)-6, IL-8, insulin-like growth factor-1 (IGF-1), and cortisol were analyzed, and the decrease in myostatin resulting from the administration of tannase-treated green tea extract was found to be related to the change in muscle mass and strength. In summary, oral administration of tannase-treated green tea extract containing antioxidants without concomitant exercise can improve muscle mass and strength and may have therapeutic benefits in age-related muscle function decline.

scholarly journals Promising Role of Growth Hormone-Boosting Peptide in Regulating the Expression of Muscle-Specific Genes and Related MicroRNAs in Broiler Chickens

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1906
Author(s):  
Doaa Ibrahim ◽  
Hanan S. Al-Khalaifah ◽  
Ahmed Abdelfattah-Hassan ◽  
Haitham Eldoumani ◽  
Safaa I. Khater ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Growth Hormone ◽  
Muscle Development ◽  
Expression Profiles ◽  
Control Group ◽  
Meat Production ◽  
Broiler Chicks ◽  
Skeletal Muscle Development ◽  
Feed Conversion ◽  
D 20

Appropriate skeletal muscle development in poultry is positively related to increasing its meat production. Synthetic peptides with growth hormone-boosting properties can intensify the effects of endogenous growth hormones. However, their effects on the mRNA and miRNA expression profiles that control muscle development post-hatching in broiler chicks is unclear. Thus, we evaluated the possible effects of synthetic growth hormone-boosting peptide (GHBP) inclusion on a chicken’s growth rate, skeletal muscle development-related genes and myomiRs, serum biochemical parameters, and myofiber characteristics. A total of 400 one-day-old broiler chicks were divided into four groups supplied with GHBP at the levels of 0, 100, 200 and 300 μg/kg for 7 days post-hatching. The results showed that the highest levels of serum IGF-1 and GH at d 20 and d 38 post-hatching were found in the 200 μg/kg GHBP group. Targeted gene expression analysis in skeletal muscle revealed that the GHBP effect was more prominent at d 20 post-hatching. The maximum muscle development in the 200 μg/kg GHBP group was fostered by the upregulation of IGF-1, mTOR, myoD, and myogenin and the downregulation of myostatin and the Pax-3 and -7 genes compared to the control group. In parallel, muscle-specific myomiR analysis described upregulation of miR-27b and miR-499 and down-regulation of miR-1a, miR-133a, miR-133b, and miR-206 in both the 200 and 300 μg/kg GHBP groups. This was reflected in the weight gain of birds, which was increased by 17.3 and 11.2% in the 200 and 300 μg/kg GHBP groups, respectively, when compared with the control group. Moreover, the maximum improvement in the feed conversion ratio was achieved in the 200 μg/kg GHBP group. The myogenic effects of GHBP were also confirmed via studying myofiber characteristics, wherein the largest myofiber sizes and areas were achieved in the 200 μg/kg GHBP group. Overall, our findings indicated that administration of 200 μg/kg GHBP for broiler chicks could accelerate their muscle development by positively regulating muscle-specific mRNA and myomiR expression and reinforcing myofiber growth.

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