scholarly journals A novel method for production of peroxidase enzyme from garden radish root source using a spouted bed reactor modeling and optimization approach

Author(s):  
Shahrbanoo Hamedi ◽  
M. Mehdi Afsahi ◽  
Ali Riahi-Madvar

Abstract The main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study for the first time, the spouted bed reactors were utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase enzyme. The response surface methodology (RSM) was used to evaluate the individual and interactive effects of main parameters (the inlet air temperature (T) and the air flow rate (Q)) on the residual enzyme activity (REA). The maximum REA of 38.7% was obtained at the T= 50 °C and Q= 1.4. In order to investigate the drying effect on the catalytic activity, the optimum reaction conditions (pH and temperature), as well as kinetic parameters, were investigated for the fresh and dried enzyme extracts (FEE and DEE). The obtained results showed that optimum pH was decreased about 12.3% while 85.7% increase was observed in optimum temperature of DEE compare to FEE. Moreover, kinetic parameters, thermal-stability, and shelf life of the enzyme were considerably improved after drying by the spouted bed. Overall, the results confirmed that a spouted bed reactor can be used as a promising method for drying heat-sensitive materials such as peroxidase enzyme.

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shahrbanoo Hamedi ◽  
M. Mehdi Afsahi ◽  
Ali Riahi-Madvar ◽  
Ali Mohebbi

AbstractThe main advantages of the dried enzymes are the lower cost of storage and longer time of preservation for industrial applications. In this study, the spouted bed dryer was utilized for drying the garden radish (Raphanus sativus L.) root extract as a cost-effective source of the peroxidase enzyme. The response surface methodology (RSM) was used to evaluate the individual and interactive effects of main parameters (the inlet air temperature (T) and the ratio of air flow rate to the minimum spouting air flow rate (Q)) on the residual enzyme activity (REA). The maximum REA of 38.7% was obtained at T = 50 °C and Q = 1.4. To investigate the drying effect on the catalytic activity, the optimum reaction conditions (pH and temperature), as well as kinetic parameters, were investigated for the fresh and dried enzyme extracts (FEE and DEE). The obtained results showed that the optimum pH of DEE was decreased by 12.3% compared to FEE, while the optimum temperature of DEE compared to FEE increased by a factor of 85.7%. Moreover, kinetic parameters, thermal-stability, and shelf life of the enzyme were considerably improved after drying by the spouted bed. Overall, the results confirmed that a spouted bed reactor can be used as a promising method for drying heat-sensitive materials such as peroxidase enzyme.


2021 ◽  
Vol 11 (8) ◽  
pp. 3713
Author(s):  
Yun-Hee Choi ◽  
Da-An Huh ◽  
Ju-Yeon Lee ◽  
Ji Yoon Choi ◽  
Kyong Whan Moon

Physical and chemical cleaning for the removal of indoor microorganisms, which can cause allergic reactions and respiratory diseases, is labor-intensive and time-consuming. An ultra-low volume (ULV) sprayer, a newly introduced device to inactivate pathogenic microorganisms, allows the disinfectant particles to reach hard-to-reach spaces indoors and is more cost-effective than the existing methods. However, few studies have been conducted to verify the efficiency of the ULV sprayer. Here, we verified the disinfection efficiency of the ULV sprayer for inactivating total bacteria present on indoor surfaces, considering the factors affecting bacteria inactivation, and presented the optimal ULV sprayer usage conditions to achieve the highest disinfection efficiency depending on room size. The total bacteria removal efficiency was high (range: 0.56–2.46 log10 reductions), including hard-to-reach spaces. A response surface model was developed to identify the individual and interactive effects of the disinfectant concentration, spray amount, and room size on total bacteria disinfection efficiency. These three variables had interactive effects on the total bacteria disinfection efficiency. The experimental data were fitted to a second-order polynomial model, with high coefficients of determination (R2) for all models (R2 > 0.82). The optimum conditions were a spray amount of 3.08–6.40 L in 160 m3, 3.78–7.22 L in 230 m3, and 5.68–8 L in 300 m3 surface area when using dilution rates of 100 times. These conditions predicted a bacterial disinfection efficiency of >1.10 log10 reductions (92%) on all surfaces. Our results clearly indicate that the ULV sprayer effectively inactivates total bacteria present on indoor surfaces.


Energies ◽  
2020 ◽  
Vol 13 (22) ◽  
pp. 6069
Author(s):  
Sajjad Haider ◽  
Peter Schegner

It is important to understand the effect of increasing electric vehicles (EV) penetrations on the existing electricity transmission infrastructure and to find ways to mitigate it. While, the easiest solution is to opt for equipment upgrades, the potential for reducing overloading, in terms of voltage drops, and line loading by way of optimization of the locations at which EVs can charge, is significant. To investigate this, a heuristic optimization approach is proposed to optimize EV charging locations within one feeder, while minimizing nodal voltage drops, cable loading and overall cable losses. The optimization approach is compared to typical unoptimized results of a monte-carlo analysis. The results show a reduction in peak line loading in a typical benchmark 0.4 kV by up to 10%. Further results show an increase in voltage available at different nodes by up to 7 V in the worst case and 1.5 V on average. Optimization for a reduction in transmission losses shows insignificant savings for subsequent simulation. These optimization methods may allow for the introduction of spatial pricing across multiple nodes within a low voltage network, to allow for an electricity price for EVs independent of temporal pricing models already in place, to reflect the individual impact of EVs charging at different nodes across the network.


Author(s):  
Jinbao Zhang ◽  
Jaeyoung Lee

Abstract This study has two main objectives: (i) to analyse the effect of travel characteristics on the spreading of disease, and (ii) to determine the effect of COVID-19 on travel behaviour at the individual level. First, the study analyses the effect of passenger volume and the proportions of different modes of travel on the spread of COVID-19 in the early stage. The developed spatial autoregressive model shows that total passenger volume and proportions of air and railway passenger volumes are positively associated with the cumulative confirmed cases. Second, a questionnaire is analysed to determine changes in travel behaviour after COVID-19. The results indicate that the number of total trips considerably decreased. Public transport usage decreased by 20.5%, while private car usage increased by 6.4%. Then the factors affecting the changes in travel behaviour are analysed by logit models. The findings reveal significant factors, including gender, occupation and travel restriction. It is expected that the findings from this study would be helpful for management and control of traffic during a pandemic.


Sensors ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1301
Author(s):  
Federico Cavedo ◽  
Parisa Esmaili ◽  
Michele Norgia

A low-cost optical reflectivity sensor is proposed in this paper, able to detect the presence of objects or surface optical properties variations, at a distance of up to 20 m. A collimated laser beam is pulsed at 10 kHz, and a synchronous digital detector coherently measures the back-diffused light collected through a 1-inch biconvex lens. The sensor is a cost-effective solution for punctual measurement of the surface reflection at different distances. To enhance the interference immunity, an algorithm based on a double-side digital baseline restorer is proposed and implemented to accurately detect the amplitude of the reflected light. As results show, the sensor is robust against ambient light and shows a strong sensitivity on a wide reflection range. The capability of the proposed sensor was evaluated experimentally for object detection and recognition, in addition to dedicated measurement systems, like remote encoders or keyphasors, realized far from the object to be measured.


Water ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 1886
Author(s):  
Arezoo Zahediasl ◽  
Amin E. Bakhshipour ◽  
Ulrich Dittmer ◽  
Ali Haghighi

In recent years, the concept of a centralized drainage system that connect an entire city to one single treatment plant is increasingly being questioned in terms of the costs, reliability, and environmental impacts. This study introduces an optimization approach based on decentralization in order to develop a cost-effective and sustainable sewage collection system. For this purpose, a new algorithm based on the growing spanning tree algorithm is developed for decentralized layout generation and treatment plant allocation. The trade-off between construction and operation costs, resilience, and the degree of centralization is a multiobjective problem that consists of two subproblems: the layout of the networks and the hydraulic design. The innovative characteristics of the proposed framework are that layout and hydraulic designs are solved simultaneously, three objectives are optimized together, and the entire problem solving process is self-adaptive. The model is then applied to a real case study. The results show that finding an optimum degree of centralization could reduce not only the network’s costs by 17.3%, but could also increase its structural resilience significantly compared to fully centralized networks.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Robert Christie ◽  
Adrian Abel

Abstract This chapter provides an overview of the structural and synthetic chemistry, and the industrial applications, of dioxazine pigments, a small group of high performance organic pigments. The color violet (or purple) has frequently assumed a prominent position in history, on account of its rarity and cost. The natural colorant Tyrian purple and the first synthetic textile dye, Mauveine, are prime examples of this unique historical feature. CI Pigment Violet 23, also referred to as Dioxazine Violet or Carbazole Violet, is one of the most universally used organic pigments, by far the most important industrial pigment in the violet shade area. Dioxazine Violet is also unique as the dominant industrial violet pigment providing a brilliant, intense violet color and an excellent all-round set of fastness properties. The pigment has a polycyclic molecular structure, originally described wrongly as a linear arrangement, and later shown to adopt an S-shaped arrangement on the basis of X-ray structural analysis. Two other dioxazine pigments are of rather lesser importance. The synthesis and manufacturing route to CI Pigment Violet 23 is described in the review. Finally, a survey of the principal current applications of the individual dioxazine pigments is presented.


2021 ◽  
Vol 9 (5) ◽  
pp. 478
Author(s):  
Hao Chen ◽  
Weikun Li ◽  
Weicheng Cui ◽  
Ping Yang ◽  
Linke Chen

Biomimetic robotic fish systems have attracted huge attention due to the advantages of flexibility and adaptability. They are typically complex systems that involve many disciplines. The design of robotic fish is a multi-objective multidisciplinary design optimization problem. However, the research on the design optimization of robotic fish is rare. In this paper, by combining an efficient multidisciplinary design optimization approach and a novel multi-objective optimization algorithm, a multi-objective multidisciplinary design optimization (MMDO) strategy named IDF-DMOEOA is proposed for the conceptual design of a three-joint robotic fish system. In the proposed IDF-DMOEOA strategy, the individual discipline feasible (IDF) approach is adopted. A novel multi-objective optimization algorithm, disruption-based multi-objective equilibrium optimization algorithm (DMOEOA), is utilized as the optimizer. The proposed MMDO strategy is first applied to the design optimization of the robotic fish system, and the robotic fish system is decomposed into four disciplines: hydrodynamics, propulsion, weight and equilibrium, and energy. The computational fluid dynamics (CFD) method is employed to predict the robotic fish’s hydrodynamics characteristics, and the backpropagation neural network is adopted as the surrogate model to reduce the CFD method’s computational expense. The optimization results indicate that the optimized robotic fish shows better performance than the initial design, proving the proposed IDF-DMOEOA strategy’s effectiveness.


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