scholarly journals Effect of intestinal flora on the formation of endometritis of sow

2020 ◽  
Author(s):  
Ling Zhang ◽  
Linkang Wang ◽  
Yimin Dai ◽  
Tianyu Tao ◽  
Jingqi Wang ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Experimental Result ◽  
Intestinal Microbes ◽  
Birth Canal ◽  
Significant Difference ◽  
Vaginal Secretions ◽  
Fresh Feces ◽  
The Relationship

Abstract Background Aberration of birth canal microbiota is one of the most important factors in the etiology of sow endomentritis. Nevertheless, reports about the structure and composition of birth canal microbiota in endomentritis sow and their relationship with intestinal microbes is limited. Therefore, understanding the relationship between birth canal microbiota and intestinal microbiota of the host has become exceedingly crucial. Results In this study, 4 healthy and 4 endomentritis sows were selected basing on whether the sow had endometritis or not in a farm of China. The microflora of their birth canal secretions and fresh feces were analyzed via sequencing the V3 + V4 region of bacterial 16S rDNA gene. The results showed that the significant difference between endometritis and healthy sows birth canal flora in the composition and abundance. Sow endomentritis was associated with increasing in the relative abundance of Porphyromonas, Clostridium_sensu_stricto_1, Streptococcus, Ezakiella, Fusobacterium, Actinobacillus, Bacteroides, and Prevotella as well as Anaerococcus. On the contrary, the majority of beneficial bacteria that belonging to Firmicutes phylum (e.g., Lactobacillus and Enterococcus ) declined in endomentritis sow. The increased relative abundance of Porphyromonas in the vaginal secretions might correlate with the decrease of Lactobacillusin the feces of endometritis sows. Moreover, the experimental result also found that some intestinal bacteria (such as Escherichia-Shigella and Bacteroides) may be bound up with the onset of sow endometritis. Conclusion Sow endometritis is closely related to the microbiota of birth canal, and that some intestinal bacteria may promote the onset of endometritis. The above results can supply a theoretical basis to research the pathogenesis of endometritis and the microbiota of sow's birth canal and gut.

scholarly journals Effect of Sow Intestinal Flora on the Formation of Endometritis

2021 ◽  
Vol 8 ◽  
Author(s):  
Ling Zhang ◽  
Linkang Wang ◽  
Yimin Dai ◽  
Tianyu Tao ◽  
Jingqi Wang ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Sensu Stricto ◽  
Beneficial Bacteria ◽  
Birth Canal ◽  
Significant Difference ◽  
Fresh Feces ◽  
The Common ◽  
The Relationship

Endometritis is the main cause of decreased reproductive performance of sows, while one of the most important factors in the etiology of sow endometritis is an aberration of birth canal microbiota. Therefore, people began to pay attention to the microbiota structure and composition of the birth canal of sows with endometritis. Interestingly, we found that the risk of endometritis was increased in the sows with constipation in clinical practice, which may imply that the intestinal flora is related to the occurrence of endometritis. Therefore, understanding the relationship between birth canal microbiota and intestinal microbiota of the host has become exceptionally crucial. In this study, the microbiota of birth canal secretions and fresh feces of four healthy and four endometritis sows were analyzed via sequencing the V3 + V4 region of bacterial 16S ribosomal (rDNA) gene. The results showed a significant difference between endometritis and healthy sows birth canal flora in composition and abundance. Firmicutes (74.36%) and Proteobacteria were the most dominant phyla in birth canal microbiota of healthy sows. However, the majority of beneficial bacteria that belonging to Firmicutes phylum (e.g., Lactobacillus and Enterococcus) declined in endometritis sow. The abundance of Porphyromonas, Clostridium sensu stricto 1, Streptococcus, Fusobacterium, Actinobacillus, and Bacteroides increased significantly in the birth canal microbiota of endometritis sows. Escherichia–Shigella and Bacteroides were the common genera in the birth canal and intestinal flora of endometritis sows. The abundance of Escherichia–Shigella and Bacteroides in the intestines of sows suffering from endometritis were significantly increased than the intestinal microbiota of the healthy sows. We speculated that some intestinal bacteria (such as Escherichia–Shigella and Bacteroides) might be bound up with the onset of sow endometritis based on intestinal microbiota analysis in sows with endometritis and healthy sows. The above results can supply a theoretical basis to research the pathogenesis of endometritis and help others understand the relationship with the microbiota of sow's birth canal and gut.

scholarly journals Gut microbiota: an overlooked factor that plays a significant role in osteoporosis

2019 ◽  
Vol 47 (9) ◽  
pp. 4095-4103 ◽  
Author(s):  
Meng-lei Hao ◽  
Guang-yao Wang ◽  
Xiao-qin Zuo ◽  
Chan-juan Qu ◽  
Bo-chen Yao ◽  
...  
Keyword(s):  
Bone Health ◽  
Eating Habits ◽  
Close Association ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Living Environment ◽  
Intestinal Microbes ◽  
Negative Effect ◽  
New Ideas ◽  
The Relationship

Gut microbes are known as the body’s second gene pool. Symbiotic intestinal bacteria play a major role in maintaining balance in humans. Bad eating habits, antibiotic abuse, diseases, and a poor living environment have a negative effect on intestinal flora. Abnormal intestinal microbes are prone to cause a variety of diseases, affecting life expectancy and long-term quality of life, especially in older people. Several recent studies have found a close association between intestinal microorganisms and osteoporosis. The potential mechanism of intestinal flora affecting bone formation or destruction by mediating nitric oxide, the immune and endocrine systems, and other factors is briefly described in this review. All of these factors may be responsible for the intestinal flora that causes osteoporosis. Studying the relationship between intestinal flora and bone health not only provides new ideas for studying the role of intestinal microorganism in osteoporosis, but also provides a new therapeutic direction for clinically refractory osteoporosis. Study of the relationship between intestinal microbiota and osteoporosis is important for maintaining bone health and minimizing osteoporosis.

scholarly journals Formulation of traditional Chinese medicine and its application on intestinal flora of constipated rats

2020 ◽  
Author(s):  
Minghan Huang ◽  
Sihan Li ◽  
Youcheng He ◽  
Haiou Zhang ◽  
Ruoying Xu ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Relative Abundance ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Control Group ◽  
Symbiotic Relationships ◽  
Study Results ◽  
Significant Difference ◽  
Abundance And Diversity

Abstract In this study, the self-extracted constipation treatment of traditional Chinese medicine extracts was applied to constipated rats. To explore the mechanism and role of the Chinese medicine for the treatment of constipation, the 16S rRNA sequencing and qRT-PCR technology were used to analyze the abundance and diversity of intestinal flora in rats. We found that the relative abundance of Firmicutes in the intestinal flora of rats with constipation was significantly higher than that in the control group, and the Bacteroides was significantly lower than that in the control group, while the relative abundance of Firmicutes was significantly changed after taking a certain dose of Chinese medicine, and reduced the relative abundance of Lachnospiraceae, and greatly increased the relative abundance of Lactobacillus, enhanced the symbiotic relationships of Lactobacillus with other intestinal flora. There was no significant difference in the total copies of intestinal bacteria between the constipated rats and the control group. The total copies of intestinal bacteria in the constipated rats decreased after taking the traditional Chinese medicine. Finally, this study results provides a theoretical basis for the treatment and understand the mechanism and effect of traditional Chinese medicine on rate constipation.

PCR-DGGE Analysis of Bacterial Diversity of Intestinal System in Hyperlipidemia Rats

2013 ◽  
Vol 726-731 ◽  
pp. 898-901
Author(s):  
Ri Na Wu ◽  
Xiao Meng Pang ◽  
Xi Yan Wang ◽  
Jun Rui Wu
Keyword(s):  
Bacterial Diversity ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Control Group ◽  
Rrna Gene ◽  
Dgge Analysis ◽  
Gradient Gel Electrophoresis ◽  
The Relationship ◽  
Insight Into

Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene has been regarded as one of powerful tools for gaining insight into the bacterial diversity of intestinal system. In the present study, hyperlipidemia model was constructed in rat according to the tests of blood lipids. Fecal samples of rats were collected after 60d feeding, and DGGE was used to investigate the diversities of intestinal bacteria in the artificially-induced hyperlipidemia rats and normal rats. The results showed that two patterns had similarities, but there were also some different bacteria communities. Moreover, control group had much more bands than model group on gel, showing species in intestinal of model rats might be deduced by hyperlipidemia. It will be helpful to explore the relationship between hyperlipidemia and intestinal flora.

scholarly journals Gut–Lung Axis: Microbial Crosstalk in Pediatric Respiratory Tract Infections

2021 ◽  
Vol 12 ◽  
Author(s):  
Wenxia Zhu ◽  
Yilin Wu ◽  
Hui Liu ◽  
Caini Jiang ◽  
Lili Huo
Keyword(s):  
Gut Microbiota ◽  
Respiratory Tract ◽  
Respiratory Tract Infections ◽  
Respiratory Infections ◽  
Intestinal Flora ◽  
Intestinal Microbes ◽  
Important Regulator ◽  
Tract Infections ◽  
The Relationship ◽  
Gut Microbiota Composition

The gut microbiota is an important regulator for maintaining the organ microenvironment through effects on the gut-vital organs axis. Respiratory tract infections are one of the most widespread and harmful diseases, especially in the last 2 years. Many lines of evidence indicate that the gut microbiota and its metabolites can be considered in therapeutic strategies to effectively prevent and treat respiratory diseases. However, due to the different gut microbiota composition in children compared to adults and the dynamic development of the immature immune system, studies on the interaction between children’s intestinal flora and respiratory infections are still lacking. Here, we describe the changes in the gut microbiota of children with respiratory tract infections and explain the relationship between the microbiota of children with their immune function and disease development. In addition, we will provide perspectives on the direct manipulation of intestinal microbes to prevent or treat pediatric respiratory infections.

A study of the correlation between chalazion and intestinal flora in chinese children

2020 ◽  
Author(s):  
Yuanyue Cui ◽  
Lei Song ◽  
Xie Li ◽  
Ting Qiu ◽  
Jing Jin ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Diversity Index ◽  
Bacterial Species ◽  
Intestinal Flora ◽  
Meibomian Gland ◽  
Control Group ◽  
Healthy Children ◽  
Gut Flora ◽  
Microbial Composition ◽  
Significant Difference

Abstract BackgroundChalazion is a chronic inflammatory granuloma of the meibomian gland formed on the basis of the obstruction of the meibomian gland drainage duct and the retention of secretions. It is one of the most common clinically eye diseases in children. Chronic inflammation of the meibomian glands is responsible for this disease, and the gut flora is thought to be involved in the inflammatory process. In this study, we investigated the relationship between intestinal microbial composition and children's chalazion.MethodsFecal samples were collected from 21 children with chalazion and 26 healthy children. DNA was extracted from fecal stool samples and 16S rRNA sequences in the gut flora were detected by using second second-generation sequencing technology. The results were used to compare the composition of the microbiome between patients and healthy controls.ResultsAccording to Alpha Diversity and Beta Diversity analysis, we found that there was no significant difference in bacteria diversity and relative abundance between the two groups. We compared the flora of the control group and the diseased group through Lefse analysis, and screened out 11 different species. Based on the absolute abundance of species, 43 different species were selected. Anosim analysis and metastats analysis were used to compare the flora of the control group and the diseased group again. At the species level, we found that gut_metagenome and human_gut_metagenome are the common differences in species levels obtained from the above analysis. Finally, corrplot correlation analysis was performed, suggesting that gut_metagenome has a great correlation with the number, ulceration, and recurrence of chalazion in children.ConclusionsThere was no significant difference in the diversity index and relative abundance of flora in children with chalazion compared with healthy children, but there were significant differences in some bacterial species. The gut_metagenome strains identified in this study were significantly related to the growth, ulceration, and relapse of children with chalazion. It is suggested that gut_metagenome may be a microbiological indicator which is independent of clinicopathologic factors but associated with chalazion disease .* These authors have contributed equally to this work.

scholarly journals Intestinal microbiota has important effect on severity of hand foot and mouth disease in children

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Chenguang Shen ◽  
Yi Xu ◽  
Jingkai Ji ◽  
Jinli Wei ◽  
Yujin Jiang ◽  
...  
Keyword(s):  
Enterovirus 71 ◽  
Foot And Mouth Disease ◽  
Intestinal Bacteria ◽  
Mouth Disease ◽  
Childhood Illness ◽  
Healthy Controls ◽  
Intestinal Microorganisms ◽  
Significant Difference ◽  
Foot And Mouth ◽  
The Relationship

Abstract Background The incidence of hand foot and mouth disease (HFMD) has increased in recent years, making it a very common childhood illness worldwide. The relationship between different enterovirus genotypes and disease severity is not clearly understood. Given that enteroviruses are transmitted through the gastrointestinal tract, we hypothesized that variation in intestinal microorganisms of the host might play a role in the prognosis of HFMD. Methods We carried out a meta-transcriptomic-wide association study of fecal samples obtained from a cohort of children (254 patients, 227 tested positive for enterovirus, including 16 patients co-infectied with 2 kinds of enterovirus) with mild and severe HFMD and healthy controls. Results We found there was no significant difference in the amount of each virus type between the mild and severe cases. Genes of enterovirus 71 (EV71) and coxsackievirus A (CV-A) from the severe and mild cases did not show significant clustering. Clostridium sp. L2-50 and Bacteroides stercoris ATCC 43183 were enriched in the guts of children with severe HFMD and KEGG enrichment was found between mild and severe cases. Conclusions Intestinal microorganisms appear to interact with enterovirus to determine the progression of HFMD. Genes of Bacteroides and Clostridium may be used as predictive markers for a more efficient prognosis and intervention. The enrichment of intestinal bacteria genes with functions may facilitate the development of severe symptoms for HFMD patients.

scholarly journals Effect of the HXBM408 bacteria on rat intestinal bacterial diversity and the metabolism of soybean isoflavones

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0253728
Author(s):  
Li Xiaobin ◽  
Xie Jinglong ◽  
Zhao Fang ◽  
Wang Chenchen ◽  
Yang Kailun
Keyword(s):  
Relative Abundance ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Control Group ◽  
Soybean Isoflavones ◽  
Short Period ◽  
The Difference ◽  
Experimental Group ◽  
Degradation Ability ◽  
Over Time

The purpose of this study was to investigate the effect of the HXBM408 bacteria on the diversity of rat intestinal bacteria and the metabolism of soybean isoflavones. The control group was administered sterilized water and daidzein by gavage for 7 days. Conversely, the experimental group was administered HXBM408 solution and daidzein by gavage for 7 days. The content of the daidzein metabolite equol in rat feces in the experimental group was significantly higher than that in the control group (P < 0.05) on the 7th and 14th days. However, the content of daidzein and its metabolites in feces was not significantly different (P > 0.05). On the 7th day, the relative abundance of Streptococcus in the feces of the experimental group was significantly higher than that of the control group (P < 0.05), but the difference disappeared over time (P > 0.05). In the intestinal digesta of rats, the proteobacteria of the experimental group was significantly lower than those of the control group (P < 0.05). HXBM408 can increase the degradation ability of soybean isoflavones in a short period after ingestion, increase the number of beneficial intestinal flora, and improve the structure of the flora.

scholarly journals Comparison and Analysis of Gut Microbiota in Children With IgA Vasculitis With Different Clinical Symptoms

2022 ◽  
Vol 9 ◽  
Author(s):  
Meng Li ◽  
Xiaoming Wang ◽  
Xingjie Lin ◽  
Xiuju Bian ◽  
Rui Jing ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Digestive Tract ◽  
Relative Abundance ◽  
Clinical Symptoms ◽  
Microbial Community Composition ◽  
Intestinal Flora ◽  
Healthy Children ◽  
Iga Vasculitis ◽  
Sequencing Data ◽  
Intestinal Microbes

Background: Henoch-Schönlein purpura, now called immunoglobulin A (IgA) vasculitis, is a common autoimmune disease in children, its association with gut microbiota composition remains unknown.Methods: The collected cases were divided into three groups: G1 group of simple skin type, G2 group with no digestive tract expression, G3 group of mixed digestive tract, and C group of healthy children. The fecal samples of each group of children were collected and the sequencing data was processed and analyzed. The dilution curve reflected the reasonableness of the amount of sequencing data.Results: The number of species composition sequences in the G1, G2 and G3 groups was lower than that in the C group, especially for the G2 and G3 groups. The four most abundant bacteria were Bacteroidetes, Firmicutes, Proteobacteria and Actinobacteria. The relative abundance of Proteobacteria in the G2 and G3 groups was significantly higher than that in the G1 and C groups, while the relative abundance of Actinobacteria was significantly reduced, and the relative abundance of Actinobacteria in the G1 group was lower than that in the C group. Principal component analysis of the UPGMA clustering tree and each group of samples showed that the microbial community composition of the same group of samples was similar.Conclusions: The abundance of intestinal microbes in children with IgA vasculitis is lower than in normal children. Bacteroidetes, Firmicutes, Proteobacteria and Actinobacteria are the four most abundant bacteria in the intestinal flora of children. Proteobacteria and Actinobacteria are associated with organ involvement in IgA vasculitis.

scholarly journals Formulation of traditional Chinese medicine and its application on intestinal flora of constipated rats

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Sihan Li ◽  
Youcheng He ◽  
Haiou Zhang ◽  
Rong Zheng ◽  
Ruoying Xu ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Relative Abundance ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Symbiotic Relationships ◽  
Study Results ◽  
Rrna Sequencing ◽  
Pcr Technology

AbstractIn this study, the self-extracted constipation treatment of traditional Chinese medicine extracts was applied to constipated rats. To explore the mechanism and role of the Chinese medicine for the treatment of constipation, the 16S rRNA sequencing and qRT-PCR technology were used to analyze the intestinal flora. We found that the relative abundance of Firmicutes with constipation was significantly higher accounted for 86.7%, while the gut microbiota was significantly changed after taking a certain dose of Chinese medicine, greatly increased the relative abundance of Lactobacillus accounted for 23.1%, enhanced the symbiotic relationships of Lactobacillus with other intestinal flora. The total copies of intestinal bacteria in the constipated rats decreased after taking the traditional Chinese medicine. Finally, this study results provides a theoretical basis for the treatment and understand the mechanism and effect of traditional Chinese medicine on rate constipation.

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