scholarly journals Experimental Study On Antibacterial Properties of Iodine-Coated Implant in Acute Osteomyelitis Model

2021 ◽  
Author(s):  
Xiqi Zhuang ◽  
Weibing Yang ◽  
Yongquan Zhang ◽  
Wanming Wang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Early Stage ◽  
Antibacterial Properties ◽  
Bacterial Suspension ◽  
Acute Osteomyelitis ◽  
Marrow Cavity ◽  
Coated Implant ◽  
K Wires ◽  
Scanning Electron

Abstract Purpose We have developed a iodine-coated implant and evaluated its antibacterial properties against Gram-negative bacteria by constructing an experimental osteomyelitis model.Methods In this study, 16 titanium Kirschner-wires were selected, of which 8 titanium K-wires were treated with iodine on the surface by electrophoretic deposition with PVP-I solution. In our study, the standard strain of Escherichia coli (ATCC 25922) was selected, and 16 New Zealand rabbits were selected. There were 8 rabbits in the iodine-coated group and 8 rabbits in the non-iodine-coated group. All animals were drilled in the left proximal tibia after successful anesthesia.After that,the iodine-coated group was implanted with iodine-coated titanium K-wires.Then, 25ul of 2×108CFU/ml bacterial suspension was injected into the bone marrow cavity with pipette gun, and the bone hole was sealed with bone wax.They were reared in cages for 1 week after operation, and evaluated and analyzed by microbiology, histopathology, scanning electron microscopy, etc.Results The results of gross wound score and microbiology showed that the wound infection of the iodine-coated group was less severe than that of the non-iodine-coated group, which was confirmed by the histopathological results. Scanning electron microscopy and confocal microscopy showed that the amount of bacteria on the surface of iodine-coated K-wires was significantly lower than that non-iodine-coated K-wires. Conclusions In this study, we have verified that the iodine-coated titanium implant could effectively inhibit E. coli infection in the early stage of infection by constructing an acute osteomyelitis model.

scholarly journals Antibacterial activity of zinc oxide nanoparticles obtained by pulsed laser ablation in water and air

2018 ◽  
Vol 243 ◽  
pp. 00017 ◽  
Author(s):  
Daria Goncharova ◽  
Ekaterina Gavrilenko ◽  
Anna Nemoykina ◽  
Valery Svetlichnyi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Antibacterial Activity ◽  
Laser Ablation ◽  
Zno Nanoparticles ◽  
Antibacterial Properties ◽  
Pulsed Laser ◽  
Pulsed Laser Ablation ◽  
Composition And Structure ◽  
Scanning Electron

The paper studies physicochemical and antibacterial properties of ZnO nanoparticles obtained by pulsed laser ablation in water and air. Their composition and structure were studied by X-ray diffraction, transmission and scanning electron microscopy. Antibacterial activity of the nanoparticles was examined by its affection on Gram-positive Staphylococcus aureus (S.aureus). The dependence of nanoparticles’ physical and chemical antibacterial properties on the conditions of the ablation was shown. The model materials for the antibacterial bandage were made of cotton, filter paper and biodegradable polymer scaffolds (poly-l-lactide acid), and then they were coated with the obtained ZnO nanoparticles. The model bandage materials were examined by the scanning electron microscopy method and their antibacterial activity (ISO 20743:2013) was determined. High activity of all the samples against S.aureus was proved.

Preparation and study on the optical, mechanical, and antibacterial properties of polylactic acid/ZnO/TiO2 shared nanocomposites

2020 ◽  
Vol 36 (3) ◽  
pp. 285-311
Author(s):  
Ali Tajdari ◽  
Amir Babaei ◽  
Alireza Goudarzi ◽  
Razie Partovi
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Scanning Electron Microscopy ◽  
Antibacterial Activity ◽  
Optical Properties ◽  
Polylactic Acid ◽  
Field Emission ◽  
Antibacterial Properties ◽  
Hydrolytic Degradation ◽  
Scanning Electron

In this research, first, ZnO nanorods were synthesized by hydrothermal method and characterized in terms of morphological and structural properties by means of field emission scanning electron microscopy, Fourier transform infrared, and X-ray diffraction techniques. Subsequently, polylactic acid/ZnO, polylactic acid/TiO2, and polylactic acid/ZnO/TiO2 nanocomposites with different percentages of nanoparticles and two different types of ZnO morphologies were prepared and their microstructural, optical, mechanical, hydrolytic degradation, and antibacterial properties were investigated. Field emission scanning electron microscopy results of polylactic acid/ZnO and polylactic acid/TiO2 samples showed a proper dispersion and nanoparticle distribution for low percentages (up to 5 wt%) and increased aggregation for the higher percentages. Besides, a large increase in the aggregation tendency was observed for combined nanoparticles (polylactic acid/ZnO/TiO2 nanocomposites). Results of the tensile test, the UV–Vis absorption tests, and the hydrolytic degradation tests of the samples showed an enhanced mechanical (approximately 55% increase in the presence of 3–5 wt% of nanoparticles) and light absorption and degradation (approximately 85% increase in the presence of 3–10 wt% of nanoparticles) for the polylactic acid by incorporating nanoparticles. It was also observed that, in addition to the quality of dispersion and distribution of nanoparticles in the polymeric matrix, the type of morphology of nanoparticles can contribute to the improvement of these properties. The cylindrical morphology of ZnO played a greater role on improving the polylactic acid mechanical properties compared to the spherical ZnO morphology (approximately 20%). On the contrary, the increased polylactic acid optical properties and degradation with ZnO spherical morphology were more pronounced (approximately 60%). Interestingly, when both ZnO and TiO2 were added, a synergistic effect in the case of UV-shielding and degradation rate and alternatively, a detrimental effect on the mechanical properties were detected. (The polylactic acid optical properties increased by about 17% and its degradation more than doubled.) Furthermore, the antibacterial activity of polylactic acid was investigated against the two Gram-positive Listeria monocytogenes and Gram-negative bacteria Escherichia coli by incorporating nanoparticles. The results indicated that as the nanoparticle percentage increases, the antibacterial activity steadily increases.

Biocidal activity of curcumin and cationic polymer possessing composites

2020 ◽  
Vol 35 (4-5) ◽  
pp. 389-398
Author(s):  
Tarık Eren ◽  
Gülay Baysal ◽  
Faik Doğan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fourier Transform ◽  
Curcuma Longa ◽  
Antibacterial Properties ◽  
Fourier Transform Infrared ◽  
Diffusion Method ◽  
X Ray Diffraction ◽  
X Ray ◽  
Scanning Electron

There is a growing interest in new type of biocidal compounds with antibacterial properties against bacteria. In this study, new antibacterial synthetic materials bearing curcumin and cationic polymers were synthesized. In the synthesis stage, the methacrylate functional cationic monomer was synthesized via the Michael addition route by using 3-acryloxy-2-hydroxypropyl methacrylate and 3-amino pyridine to obtain Monomer 1. Monomer 1 was further quaternized with hexyl bromide to obtain a cationic methacrylate functional monomer. Free-radical polymerization of Monomer 1 and methyl acrylate was conducted in the presence of azobisisobutyronitrile under dimethylformamide solvent. The composite formulation was conducted by using turmeric extract Curcuma longa (curcumin), hydroxyapatite, montmorillonite, and silver nitrate. The materials were analyzed by using the methods of X-ray diffraction, nuclear magnetic resonance, Fourier transform infrared spectroscopy, and scanning electron microscopy. The biocidal activities against the bacteria Escherichia coli, Listeria monocytogenes, Salmonella, and Staphylococcus aureus were analyzed using agar well diffusion method. From the Fourier transform infrared, X-ray diffraction, and scanning electron microscopy analysis results of the synthesized nanocomposites, it is seen that they form strong connections with the components added to the composites and form an exfoliated structure. According to the antibacterial analysis results, the nanocomposites obtained have showed a strong antibacterial resistance against E.coli, L.monocytogenes, Salmonella, and S. aureus bacteria, and the high inhibition zone areas were obtained.

Effect of sedimentary environment on shale lithofacies in the lower third member of the Shahejie Formation, Zhanhua Sag, eastern China

2017 ◽  
Vol 5 (4) ◽  
pp. T487-T501 ◽  
Author(s):  
Tingwei Li ◽  
Zhenxue Jiang ◽  
Chenlu Xu ◽  
Yuan Yuan ◽  
Pengfei Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Early Stage ◽  
Eastern China ◽  
Sedimentary Environment ◽  
Gas Production ◽  
Bohai Bay ◽  
Calcareous Shale ◽  
Shahejie Formation ◽  
Scanning Electron

Research on shale lithofacies is important for shale oil and gas production. This study focused on the lower third member of the Shahejie Formation ([Formula: see text]) in the Luo-69 well in the Zhanhua Sag, Jiyang Depression, Bohai Bay Basin, eastern China. Several methods, including thin section observations, total organic carbon (TOC) analysis, X-ray diffraction analysis, quantitative evaluations of minerals by scanning electron microscopy, major and trace-element analyses, and field emission-scanning electron microscopy, are used to investigate the effect of sedimentary environment on the type and distribution of shale lithofacies. Our research indicates that 36 types of shale lithofacies can be classified based on the TOC content, mineral composition, and sedimentary structure, of which five types are identified in the study area. The [Formula: see text] shale has a high calcareous mineral content (average of 49.64%), low clay and siliceous minerals contents (averages of 19.54% and 19.02%, respectively), a high TOC content (average of 3.00 wt%), and well-developed horizontal bedding. The sedimentary environment during the deposition of the [Formula: see text] shale in the Zhanhua Sag had a warm and moist climate, limited provenance, saline water, and strong reducibility. The sedimentary environment in the early stage had a drier climate, more limited provenance, higher salinity, and stronger reducibility than that in the later stage. Shale lithofacies can reflect a certain sedimentary environment and depositional process; similarly, a depositional environment controls the type and distribution of shale lithofacies. Due to the characteristics of the [Formula: see text] sedimentary environment, organic-rich massive mixed shale, organic-rich bedded mixed-calcareous shale, organic-rich laminated calcareous shale, and organic-fair laminated calcareous shale are developed in the [Formula: see text] formation from top to bottom.

Do molting birds renovate their skeletons as well as their plumages? Osteoporosis during the annual molt in sparrows

1992 ◽  
Vol 70 (6) ◽  
pp. 1109-1113 ◽  
Author(s):  
Mary E. Murphy ◽  
Todd G. Taruscio ◽  
James R. King ◽  
Sharon G. Truitt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bone Density ◽  
Cross Section ◽  
Unit Area ◽  
Bone Area ◽  
Subtle Change ◽  
Marrow Cavity ◽  
Void Area ◽  
Scanning Electron

Four decades ago two independent investigators reported a pronounced osteoporosis during the molt in several species of birds. With the exception of reports of statistically stable dry masses of bones in molting waterfowl this cyclic osteoporosis has received little additional attention. Using scanning electron microscopy and light microscopy we surveyed the tarsometatarsi and tibiotarsi of White-crowned Sparrows during winter and during the molt. A subtle change in bone metabolism appears coincident with the postnuptial molt in well-nourished White-crowned Sparrows. This is evident from (i) the appearance of the bone in cross section, (ii) decreases during the molt in bone density (g/mL), and (iii) increases during the molt in both the ratio of marrow-cavity area to total bone area and in the void area per unit area of cortical bone. Renovation of the bone during the molt in White-crowned Sparrows, however, appears either to proceed at a slower pace or to be less extensive than previously reported for other avian species.

scholarly journals Supplemental Material: Early formation and taphonomic significance of kaolinite associated with Burgess Shale fossils

2020 ◽  
Author(s):  
Ross Anderson ◽  
et al.
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Antibacterial Properties ◽  
Energy Dispersive ◽  
Burgess Shale ◽  
X Ray Diffraction ◽  
X Ray ◽  
Early Formation ◽  
Data Tables ◽  
Scanning Electron

Supplemental methodological details, antibacterial properties of clays, other minerals with distinct fossil/matrix distributions, summaries of mineralogy by taxon, data tables, statistical summaries, and light/scanning electron microscopy–energy-dispersive X-ray spectroscopy images of fossil specimens showing X-ray diffraction selected areas.<br>

Processibility and Thermo-Mechanical Properties of Epoxy as Reactive Plasticizer of Polyetherimide

2017 ◽  
Vol 264 ◽  
pp. 228-231
Author(s):  
Nasuha Marzuki ◽  
Muhamad Amirul Ashraf Mohd Alias ◽  
Arjulizan Rusli ◽  
Zulkifli Ahmad
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Scanning Electron Microscopy ◽  
Differential Scanning Calorimetry ◽  
Early Stage ◽  
Scanning Calorimetry ◽  
Two Phase ◽  
Dispersed Particles ◽  
Epoxy Blends ◽  
Scanning Electron

A relatively new way to improve processibility thermoplastics is via the use of crosslinkable monomers which can act as reactive plasticizers and reduce the viscosity in the early stage of processing. The monomers can polymerize and phase separate during final stage of processing thus recovering the original thermoplastics properties. In this work, the applicability of epoxy as reactive plasticizer for polyetherimide (PEI) was investigated. The properties of PEI/epoxy blends without and with curative were studied in order to determine the effect of the monomer on the processibility of the PEI and the thermo-mechanical properties of cured blends. Differential Scanning Calorimetry on blends without curative indicated single glass transition temperature (Tg) at high PEI content suggesting miscibility of the system and plasticization of PEI in the presence of epoxy while cured blends indicated two Tg due to phase separation. Scanning electron microscopy of the cured blends indicated two phase morphology with PEI dispersed particles size increased in continuous epoxy matrix with increasing PEI (up to 30wt% PEI). In blends with 40wt% PEI and more, phase inverted morphology was observed where increasing PEI content caused reduction of epoxy particle size in continuous PEI matrix.

scholarly journals Electrospun VDF-TeFE Scaffolds Modified by Copper and Titanium in Magnetron Plasma and Their Antibacterial Activity against MRSA

Technologies ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 5
Author(s):  
Arsalan D. Badaraev ◽  
Marat I. Lerner ◽  
Dmitrii V. Sidelev ◽  
Evgeny N. Bolbasov ◽  
Sergei I. Tverdokhlebov
Keyword(s):  
Electron Microscopy ◽  
Tissue Engineering ◽  
Scanning Electron Microscopy ◽  
Antibacterial Activity ◽  
Vinylidene Fluoride ◽  
Fiber Diameter ◽  
Antibacterial Properties ◽  
Argon Atmosphere ◽  
Copolymer Solution ◽  
Scanning Electron

Copolymer solution of vinylidene fluoride with tetrafluoroethylene (VDF-TeFE) was used for electrospinning of fluoropolymer scaffolds. Magnetron co-sputtering of titanium and copper targets in the argon atmosphere was used for VDF-TeFE scaffolds modification. Scanning electron microscopy (SEM) showed that scaffolds have a nonwoven structure with mean fiber diameter 0.77 ± 0.40 μm, mean porosity 58 ± 7%. The wetting angle of the original (unmodified) hydrophobic fluoropolymer scaffold after modification by titanium begins to possess hydrophilic properties. VDF-TeFE scaffold modification by titanium/copper leads to the appearance of strong antibacterial properties. The obtained fluoropolymer samples can be successfully used in tissue engineering.

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