scholarly journals Bacterial Strain for Bast Fiber Crops Degumming and Its Bio-Degumming Technique

2021 ◽  
Author(s):  
Shengwen Duan ◽  
Bingrong Xu ◽  
Lifeng Cheng ◽  
Xiangyuan Feng ◽  
Qi Yang ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Bacterial Strain ◽  
Inoculum Size ◽  
Activity Levels ◽  
Bacterial Strains ◽  
Bast Fiber ◽  
Extracellular Secretion ◽  
Apocynum Venetum ◽  
Slow Progress ◽  
Kenaf Bast

Abstract The R&D of bio-degumming technology is under a slow progress due to the shortage of proper efficient bacterial strains and processes. A degumming bacterial strain—Pectobacterium wasabiae (PW)—with broad-spectrum degumming abilities was screened out in this study. After the fermentation for 12 h, the residual gum contents of kenaf bast, ramie bast, hemp bast, flax bast, and Apocynum venetum bast were all lower than 15%. This bacterial strain could realize the simultaneous extracellular secretion of pectinase, mannase, and xylanase with the maximum enzyme activity levels of 130.25, 157.58, and 115.24 IU/mL, respectively. The optimal degumming conditions of this bacterial strain were as follows: degumming time of 12 h, bath ratio of 1:10, temperature of 33 ℃, and inoculum size of 2%. After the bio-degumming through this bacterial strain, the COD in wastewater was below 4,000 mg/L, which was over 60% lower than that in boiling-off wastewater generated by chemical degumming. This technology achieves higher efficiency, higher quality, and lower pollution.

scholarly journals Optimization of Cultural Conditions for Maximum Production of Fibrinolytic Enzymes from the Local Marine Bacterial Isolates and Evaluation of their Wound Healing and Clot Dissolving Properties

2021 ◽  
pp. 246-255
Author(s):  
K. Gowthami ◽  
R. Jaya Madhuri
Keyword(s):  
Wound Healing ◽  
Enzyme Activity ◽  
Incubation Time ◽  
Specific Activity ◽  
Blood Clot ◽  
Inoculum Size ◽  
Fibrinolytic Enzyme ◽  
Physical Parameters ◽  
Bacterial Strains ◽  
Fibrinolytic Enzymes

Fibrinolytic enzymes find necessary applications to treat and prevent cardiovascular diseases.  In this study, optimal conditions for enhancing the production of fibrinolytic enzyme from local marine bacterial strains were evaluated. The present study also focuses on screening of wound                  healing efficacy of the isolated fibrinolytic enzymes.Various physical parameters such as temperature, pH, incubation time and medium components viz. inoculum size, substrate (nitrogen and carbon) concentrations were optimized. A cultivation medium was designed using optimized conditions for mass production of fibrinolytic enzyme and specific activity of enzyme was analyzed. The maximum enzyme production was observed at 37 °C temperature, 8.0 pH,substrate concentration with 3 ml inoculum size and 32 h. of incubation time. Among the different carbon sources tested, Mannitol showed maximum enzyme activity i.e 538 U/ml.  yeast extract was found to be the best nitrogen source with an enzyme activity of 498 U/ml.  The best substrate for the production fibrinolytic enzyme was found to be kernelwith high  activity of 1056U/ml. The crude enzyme displayed potent activity and digested blood clot completely in in vitro condition and exhibited potent activity on wound healing property in macrophages.

scholarly journals Isolation of Alkalophilic Pectinolytic Bacteria and their Bio Retting Effect on Kenaf Fiber Compositions

2021 ◽  
Vol 36 (2) ◽  
pp. 156-165
Author(s):  
Munir Hossain ◽  
Shafiq Siddiquee ◽  
Vijay Kumar
Keyword(s):  
Crystallinity Index ◽  
Chemical Compositions ◽  
Test Results ◽  
Bacterial Strains ◽  
Kenaf Fiber ◽  
Bast Fiber ◽  
Gene Sequence Analysis ◽  
Paddy Land ◽  
Kenaf Bast ◽  
Kenaf Bast Fiber

Retting is the most limiting process of high-quality cellulosic kenaf bast fiber production which facilitating the separation of useable fiber from the plants' cell wall matrix. Existing traditional water retting approach confronts ineptitude and eutrophication related complications. Aiming to enhance the kenaf bio-retting process, sixty-seven alkalophilic bacterial colonies were isolated from paddy land soil sediments and kenaf retting water. These isolates were subsequently screened, of that two isolates were selected based on hyper qualitative and quantitative pectinolytic enzymatic measures. 16s rDNA gene sequence analysis revealed that both two strains were closely related to Bacillus pumilus species and designated as KRB56 and KRB22. These strains were applied in augmented non-sterile kenaf tank retting to investigate their kenaf retting efficiency and yielded fiber were analyzed for chemical compositions. Results revealed that, stains KRB56 and KRB22 significantly improve the retting process by degradation of 82.78% and 75.28% non-cellulosic gums, respectively comparing with uninoculated treatment niche (62.12%). Based on un retted raw kenaf fiber maximum fiber bundle weight was reported in MTW with 16.04% material losses, while the SW, and FW treatments showed 24.38%, and 21.03% material losses, respectively. These bacterial treated fiber samples showed thinner, smooth, and cleaner fibers surface morphology by SEM indicates sufficient non cellulosic gums (NCGs) removal comparing with URKF. Moreover, yielded fibers were examined for chemical composition, FTIR, XRD test. Results revealed that compare to un retted and un inoculated kenaf fiber, bacterial treated kenaf fiber increases cellulose portions, and their crystallinity index increases 35.50-41.30 % due to sufficient NCGs removal. This study's findings indicate that isolated alkalophilic bacterial strains KRB56 and KRB22 were effectively to be used as kenaf bio retting agents to produce quality kenaf fiber.

scholarly journals Bacterial strain for bast fiber crops degumming and its bio-degumming technique

2021 ◽  
Author(s):  
Shengwen Duan ◽  
Bingrong Xu ◽  
Lifeng Cheng ◽  
Xiangyuan Feng ◽  
Qi Yang ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Bast Fiber

scholarly journals Optimization of the Bioactivation of Isoflavones in Soymilk by Lactic Acid Bacteria

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 963
Author(s):  
Jon Kepa Izaguirre ◽  
Leire Barañano ◽  
Sonia Castañón ◽  
Itziar Alkorta ◽  
Luis M. Quirós ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Inoculum Size ◽  
Process Time ◽  
Medical Applications ◽  
Bacterial Strains ◽  
Food Industries ◽  
Glucosidase Activity ◽  
Interesting Candidate

Soybeans and soy-based products contain isoflavones which can be used for nutraceutical and medical applications. In soybeans and in unfermented soy foods, isoflavones are normally present as glycosides. Isoflavone glycosides can be enzymatically converted to isoflavone aglycones, thus releasing the sugar molecule. The effective absorption of isoflavones in humans requires the bioconversion of isoflavone glycosides to isoflavone aglycones through the activity of the enzyme β-glucosidase. The objective was to assess the capacity of 42 bacterial strains (belonging to Lactobacillus, Streptococcus and Enterococcus) to produce β-glucosidase activity. The strain that showed the highest β-glucosidase activity (Lactobacillus plantarum 128/2) was then used for the optimization of the bioconversion of genistin and daidzin present in commercial soymilk to their aglycone forms genistein and daidzein. The contribution of process parameters (temperature, inoculum size, time) to the efficiency of such bioactivation was tested. Lactobacillus plantarum 128/2 was able to completely bioactivate soymilk isoflavones under the following conditions: 25 °C temperature, 2% inoculum size and 48 h process time. These results confirm the suitability of lactic acid bacteria for the bioactivation of isoflavones present in soymilk and provide an interesting candidate (L. plantarum 182/2) for food industries to perform this transformation.

scholarly journals Low Psychosine in Krabbe Disease with Onset in Late Infancy: A Case Report

2021 ◽  
Vol 7 (2) ◽  
pp. 28
Author(s):  
Camille S. Corre ◽  
Dietrich Matern ◽  
Joan E. Pellegrino ◽  
Carlos A. Saavedra-Matiz ◽  
Joseph J. Orsini ◽  
...  
Keyword(s):  
New York ◽  
Enzyme Activity ◽  
Clinical Presentation ◽  
Neurodegenerative Disorder ◽  
New York State ◽  
Disease Onset ◽  
Early Infancy ◽  
Krabbe Disease ◽  
Activity Levels ◽  
Hematopoietic Stem

Krabbe disease (KD) is a rare inherited neurodegenerative disorder caused by a deficiency in galactocerebrosidase enzyme activity, which can present in early infancy, requiring an urgent referral for hematopoietic stem cell transplantation, or later in life. Newborn screening (NBS) for KD requires identification and risk-stratification of patients based on laboratory values to predict disease onset in early infancy or later in life. The biomarker psychosine plays a key role in NBS algorithms to ascertain probability of early-onset disease. This report describes a patient who was screened positive for KD in New York State, had a likely pathogenic genotype, and showed markedly reduced enzyme activity but surprisingly low psychosine levels. The patient ultimately developed KD in late infancy, an outcome not clearly predicted by existing NBS algorithms. It remains critical that psychosine levels be evaluated alongside genotype, enzyme activity levels, and the patient’s evolving clinical presentation, ideally in consultation with experts in KD, in order to guide diagnosis and plans for monitoring.

scholarly journals Effect of TiO2 on Selected Pathogenic and Opportunistic Intestinal Bacteria

2021 ◽  
Author(s):  
Ewa Baranowska-Wójcik ◽  
Dominik Szwajgier ◽  
Klaudia Gustaw
Keyword(s):  
Titanium Dioxide ◽  
Bacterial Strain ◽  
Intestinal Bacteria ◽  
Food Additive ◽  
Bacterial Strains ◽  
Gastrointestinal Microbiota ◽  
Food Grade ◽  
The Eu

AbstractFood-grade titanium dioxide (TiO2) containing a nanoparticle fraction (TiO2 NPs-nanoparticles) is widely used as a food additive (E171 in the EU). In recent years, questions concerning its effect on the gastrointestinal microbiota have been raised. In the present study, we examined interactions between bacteria and TiO2. The study involved six pathogenic/opportunistic bacterial strains and four different-sized TiO2 types: three types of food-grade E171 compounds and TiO2 NPs (21 nm). Each bacterial strain was exposed to four concentrations of TiO2 (60, 150, 300, and 600 mg/L TiO2). The differences in the growth of the analyzed strains, caused by the type and concentration of TiO2, were observed. The growth of a majority of the strains was shown to be inhibited after exposure to 300 and 600 mg/L of the food-grade E171 and TiO2 NPs.

Genetics of symbiosis and nitrogen fixation in legumes

1969 ◽  
Vol 172 (1029) ◽  
pp. 417-437 ◽  
Keyword(s):  
Nitrogen Fixation ◽  
Bacterial Strain ◽  
Host Plants ◽  
Strain Differences ◽  
Homogeneous Material ◽  
Developmental Sequence ◽  
Bacterial Strains ◽  
Nodule Bacteria ◽  
Microbial Genetics ◽  
Nodular Tissue

Soon after the isolation of nodule bacteria in 1888, differences were recognized in the ability of bacterial strains to form nodules on particular host plants and in the nitrogen-fixing ability of the nodules so formed. These and other symbiotic heterogeneities were attributed, sometimes correctly, to bacterial strain differences, not then thought to be open to formal genetic analysis. The realization that the host plant was an essential component of this variability came only gradually, stimulated by observations of host varietal differences and by the demand for reliable and homogeneous material for experimental work. Only within the last two decades has host variability been studied by plant breeding, and bacterial strain differences by some of the methods of microbial genetics. This review, except for a brief reference to earlier work of some historic interest, will consider only genetic problems open to investigation by these methods. The developmental sequence in all legume nodules is broadly similar. The initial infection phases are followed by the induction of the nodule, the invasion of part of the nodular tissue and culminate in bacteroid formation and nitrogen fixation; the genetics of symbiosis will be considered in this context.

scholarly journals The Molecular Basis of Quantitative Genetic Variation in Central and Secondary Metabolism in Arabidopsis

Genetics ◽  
1998 ◽  
Vol 149 (2) ◽  
pp. 739-747 ◽  
Author(s):  
Thomas Mitchell-Olds ◽  
Deana Pedersen
Keyword(s):  
Enzyme Activity ◽  
Genetic Variation ◽  
Secondary Metabolism ◽  
Genetic Correlations ◽  
Theoretical Models ◽  
Model Systems ◽  
Activity Levels ◽  
Significant Qtls ◽  
Cis Acting ◽  
Quantitative Genetic

Abstract To find the genes controlling quantitative variation, we need model systems where functional information on physiology, development, and gene regulation can guide evolutionary inferences. We mapped quantitative trait loci (QTLs) influencing quantitative levels of enzyme activity in primary and secondary metabolism in Arabidopsis. All 10 enzymes showed highly significant quantitative genetic variation. Strong positive genetic correlations were found among activity levels of 5 glycolytic enzymes, PGI, PGM, GPD, FBP, and G6P, suggesting that enzymes with closely related metabolic functions are coregulated. Significant QTLs were found influencing activity of most enzymes. Some enzyme activity QTLs mapped very close to known enzyme-encoding loci (e.g., hexokinase, PGI, and PGM). A hexokinase QTL is attributable to cis-acting regulatory variation at the AtHXK1 locus or a closely linked regulatory locus, rather than polypeptide sequence differences. We also found a QTL on chromosome IV that may be a joint regulator of GPD, PGI, and G6P activity. In addition, a QTL affecting PGM activity maps within 700 kb of the PGM-encoding locus. This QTL is predicted to alter starch biosynthesis by 3.4%, corresponding with theoretical models, suggesting that QTLs reflect pleiotropic effects of mutant alleles.

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