scholarly journals Relationship of Common Variants in PRL-3 Gene With Susceptibility and Prognosis of Prostate Cancer

2020 ◽  
Author(s):  
Shuangqing Cao ◽  
Lei Zheng
Keyword(s):  
Prostate Cancer ◽  
Expression Profiles ◽  
Prognostic Significance ◽  
Positive Association ◽  
Rank Test ◽  
Chi Square ◽  
Poor Overall Survival ◽  
Free Survival ◽  
Log Rank Test ◽  
Chi Square Test

Abstract Background: Phosphatase of regenerating liver-3 (PRL-3) was involved in a variety of malignancies. In this study, we investigated the expression pattern of PRL-3 and its prognostic significance in prostate cancer (PCa).Methods: The expression profiles of PRL-3 in 126 pairs PCa tissues and adjacent normal specimens were analyzed by quantitative rreal-time polymerase chain reaction (qRT-PCR). Chi-square test was used to estimate the association of PRL-3 level with clinicopathological factors. Survival analysis was performed by Kaplan-Meier method with log rank test. Prognosis analysis was carried out using Cox proportional hazard models.Results: PRL-3 expression was significantly up-regulated in PCa tissues compared to that in normal controls (P<0.001). Moreover, the expression of PRL-3 was positively associated with lymph node metastasis (P=0.038), Gleason score (P=0.003) and lymphovascular invasion (P=0.013). High PRL-3 expression predicted poor overall survival (OS) (log rank test, P=0.019) and dismal BCR-free survival (log rank test, P=0.021) for PCa patients. PRL-3 was an independent prognostic factor for OS (HR=2.619, 95%CI=1.129-6.076, P=0.025) and BCR-free survival (HR=2.120, 95%CI=1.095-4.105, P=0.026) in PCa.Conclusions: PRL-3 expression is elevated in PCa, and exhibits positive association with malignant tumor progression. PRL-3 may serve as a prognostic biomarker for patients with PCa.

scholarly journals Role of Bevacizumab in High-grade Meningiomas

2021 ◽  
Author(s):  
Xuexue Bai ◽  
xiangyu wang ◽  
Yiyao Cao
Keyword(s):  
Progression Free Survival ◽  
Rank Test ◽  
High Grade ◽  
Chi Square ◽  
Free Survival ◽  
Log Rank Test ◽  
Peritumoral Brain Edema ◽  
Chi Square Test

Abstract Background: To explore the role of bevacizumab (BV) in High-grade Meningiomas (HGMs) undergoing surgical treatment.Methods: Review the clinical data of 139 patients with HGMs and divide them into BV group and non- BV group according to whether they receive BV treatment. Then we compared the progression-free survival (PFS) and overall survival (OS) of the two groups.Results: The Chi-square test showed significant differences between the BV group and the non-BV group in terms of 12-month PFS (PFS-12), 36-month PFS (PFS-36), median PFS (M-PFS), 12-month OS (OS-12), 36-month OS (OS-36), and median OS (M-OS). However, there was no statistical difference between the BV group and the non-BV group in terms of 6-month PFS (PFS-6), 60-month PFS (PFS-60), and 60-month OS (OS-60). The log-rank test indicated significant differences in PFS and OS between the BV group and the non-BV group.Conclusion: The role of BV in patients with HGMs is to relieve the symptoms of peritumoral brain edema (PTBE) and prolong PFS and OS. However, whether increasing the dose of BV after surgery can improve the long-term PFS and OS of patients with HGMs needs further research.

scholarly journals Prognostic Significance of NBEAL2 in Liver hepatocellular carcinoma

2021 ◽  
Author(s):  
Yanghui Wen ◽  
Hui Su ◽  
Wuke Wang ◽  
Feng Ren ◽  
Haitao Jiang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Expression Profiles ◽  
Prognostic Significance ◽  
The Cancer Genome Atlas ◽  
Clinicopathological Parameters ◽  
Chi Square ◽  
Kaplan Meier ◽  
Chi Square Test ◽  
Liver Hepatocellular Carcinoma ◽  
The Relationship

Abstract Background: NBEAL2 is a member of the BEACH domain–containing protein (BDCP) family and little is known about the relationship between NBEAL2 and malignancy.Methods: We downloaded the Gene expression profiles and clinical data of Liver hepatocellular carcinoma(LIHC) form the Cancer Genome Atlas (TCGA) dataset. The expression difference of NBEAL2 in LIHC tissues and adjacent nontumor tissues was analyzed by R software. The relationship between NBEAL2 expression and clinicopathological parameters was evaluate by Chi-square test. The effect of NBEAL2 expression on survival were assessed by Kaplan–Meier survival analysis and Cox proportional hazards regression model. GSEA was used to explore the potential molecular mechanism of NBEAL2 in LIHC.Results: Up-regulation of NBEAL2 expression was detected in the LIHC tissue compared with adjacent nontumor tissues(P < 0.001). The chi-square test showed that no significant correlation between the expression level of NBEAL2 and various clinicopathological parameters (including T, N and M classifications) were detected. The Kaplan–Meier curves suggested that lower NBEAL2 expression was related with poor prognosis. The results of Multivariate analysis revealed that a lower expression of NBEAL2 in LIHC was an independent risk of poor overall survival (HR, 8.873; 95% CI, 1.159-67.936; P = 0.035). GSEA suggested that multiple tumor-related metabolic pathways were evidently enriched in samples with the low-NBEAL2 expression phenotype. Conlusion: NBEAL2 might act as an tumor suppressor gene in the progression of LIHC but the precise role of NBELA2 in LIHC needs further vertification.

Expression of Epstein-Barr Virus in Cell of Classical Hodgkin's Lymphoma Tumor

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 5358-5358
Author(s):  
Abrahão Elias Hallack Neto ◽  
Graziela Toledo Costa Mayrink ◽  
Luciano J. Costa ◽  
Kelli Borges dos Santos
Keyword(s):  
Prognostic Factors ◽  
Proportional Hazards ◽  
Cox Proportional Hazards ◽  
Rank Test ◽  
Chi Square ◽  
Histologic Subtype ◽  
Free Survival ◽  
Barr Virus ◽  
Log Rank Test ◽  
Epstein Barr

Abstract Introduction: The association between classical Hodgkin's Lymphoma (cHL) and tumor Epstein-Barr virus (EBV) status is well established. However, the presence of EBV within Hodgkin/Reed-Sternberg (HRS) cells and its prognosis remains controversial, with conflicting findings from studies of various regions of the world. It is considered essential to deepen the understanding of the pathogenic role of EBV in cHL and its impact in prognosis. Methods: We assessed the correlation between EBV presence in HRS and outcomes in a cohort of Brazilian patients with cHL. EBV positivity was determined by in situ hybridization (ISH) for EBV-encoded RNA (EBER) and immunohistochemistry (IMH) for viral latent membrane protein (LMP-1). All cases were histologically confirmed by an expert hematopathologist who also performed the assays for EBV identification. We examined the prognostic impact of EBV status in 29 patients with cHL. The prognostic factors by IPS (International Prognostic Score) for patients with advanced stage and the risk factors by GHSG (German Hodgkin Study Group) for patients with limited stage were correlated with EBV status tumor cells. For associations between the presence of EBV and other categorical variables, we applied Chi-square or Fisher's exact tests. For describe the effect size (ES) measures for chi-square, we used Cramér's V (V) and odds ratios (OR) with the respective 95% Confidence Intervals (CIs). To evaluate the correlation between all methods of identification of EBV status and among evaluators in histological classification, we applied the Kappa test (K), which measures the degree of agreement these assessments. Differences in OS (overall survival) and EFS (event-free survival) Kaplan-Meier survival curves between EBV-positive and EBV-negative patients were compared statistically using the log-rank test. To evaluate the impact of EBV status on event-free survival controlling for prognostic factors and unfavorable risks, we applied Cox proportional hazards regression to determine hazards ratios (HR) and associated the respective 95% CIs. Multivariate analyses included variables significant at p ≤ 0.15 in univariate models. Results: The mean age at diagnosis was 33 years. Sixty-five percent of the patients had the Nodular Sclerosis histologic subtype and 62,1% had Ann Arbor stage I or II disease at diagnosis. According to GHSG, 88,3% of early-stage patients were classified with unfavorable risk (at least one risk factor) at diagnosis. Compared to advanced-stage patients, 81,9% were considered with favorable IPS (< 4 prognostic factors) at diagnosis. HRS cells were EBV-positive in 37.9% of cases. EBV-positive cHL cases were more frequent in patients ≥ 45 years (71,4% vs. 27,3%, p =0,07). Mixed cellularity (MC) histology subtype was more common in EBV-related tumor cells (p= 0,02) and its effect-size index was medium. The correlation between all methods of identification of EBV status was 96,5% (p< 0,001; K=0.93). The correlation among evaluators in histological classification was 89,6% (p< 0,001; K=0.79). In univariate analysis, age, stage, histologic subtype, nodal involvement, extranodal disease, sex, bulky disease, laboratory data were not associated with adverse EFS (p>0,05). EBV-positive HL seemed to have better EFS than EBV-negative HL (log-rank test, p = 0,07). Cox proportional hazards model confirmed that EBV-positive tumor status and prognosis factors did not impact HL outcome. Conclusions: Despite EBV status in HRS cells not being associated with adverse prognostic factors and not influencing the overall and event-free survivals, the presence of EBV was linked to MC subtype, showing possible implication in histological subtype and worse prognosis. Disclosures Costa: Sanofi: Honoraria, Research Funding.

Hormone replacement therapy (HRT) and risk of distant recurrence in newly diagnosed ER+, node-negative (N-) breast-cancer (BC) patients: A retrospective population-based matched-cohort study.

2019 ◽  
Vol 37 (15_suppl) ◽  
pp. 6591-6591
Author(s):  
Ariel Hammerman ◽  
Ilan Feldhamer ◽  
Sari Greenberg-Dotan ◽  
Nicky Liebermann ◽  
Rinat Yerushalmi
Keyword(s):  
Breast Cancer ◽  
High Risk ◽  
Distant Recurrence ◽  
Rank Test ◽  
Matched Cohort ◽  
Newly Diagnosed ◽  
Chi Square ◽  
Log Rank Test ◽  
Chi Square Test ◽  
Risk Patients

6591 Background: Observational studies have shown an increased risk of BC with use of HRT. However, data on the prognosis of BC that develop in HRT users are inconsistent. The association between HRT use and results of the 21-gene Recurrence Score (RS) assay (Oncotype DX, Genomic Health Inc.) has not been investigated. We aimed to analyze this association, and examine the actual rate of distant recurrence or death in this population. Methods: Clalit Health Services (CHS) is the largest health maintenance organization (HMO) in Israel. We identified all CHS newly diagnosed ER+, N- breast-cancer patients, aged 45-60 that performed a RS assay between 01/2006-12/2012 and that were treated for at least three months with HRT during the eight years before BC diagnosis. A 1:4 matched-cohort analysis was performed, with matching made according to age and year of BC diagnosis. Clinical and demographic data were extracted from the CHS centralized registry for all patients. RS assay scores was grouped according to the TAILORX categorization and distribution was compared using Chi-square test. Kaplan-Meier analysis with log-rank test was performed in order to compare time to a combined outcome of distant-recurrence and mortality. Results: A cohort of 259 HRT-treated patients was identified and matched with 1001 controls, not treated with HRT. The proportions of low-risk patients (RS 0-25) and high-risk patients (RS 26-100) were 76.8% and 23.2%, respectively, within HRT-treated patients, and 80.4% and 19.6% within controls. Chi square test was not found significant (χ2= 1.634, p = 0.201). The mean follow-up time was 148.4 months for the cases and 146.9 months for controls, with log-rank test not showing a significant difference between groups. Conclusions: These data did not show significant association between HRT use and higher RS assay scores, and also did not find an association between HRT use and actual distant recurrence or death. Although the proportion of patients with high risk RS appeared to be slightly higher within HRT treated patients, this difference had not reached significance and further studies are required.

scholarly journals MicroRNA-124 alone might represent an indicator signifying cholangiocarcinoma prognosis

2020 ◽  
Author(s):  
Ning Wang ◽  
Yanni Li ◽  
Yanfang Zheng ◽  
Huoming Chen ◽  
Xiaolong Wen ◽  
...  
Keyword(s):  
Overall Survival ◽  
Cox Regression ◽  
Prognostic Biomarker ◽  
Prognostic Indicator ◽  
Rank Test ◽  
Chi Square ◽  
Log Rank Test ◽  
Kaplan Meier ◽  
Chi Square Test ◽  
Polymerase Chain

Abstract Background: Previous studies have demonstrated that microRNAs (miRNAs) played a crucial role in various diseases, including cancers. The aim of the study was to evaluate the clinical significance of miR-124 in patients with cholangiocarcinoma (CCA).Methods: The expression pattern of miR-124 was detected in CCA tissues using quantitative reserve transcription polymerase chain reaction (qRT-PCR). The correlation of miR-124 expression with clinicopathological features and overall survival of patients were explored using chi-square test, Kaplan-Meier methods and Cox regression analyses.Results: The miR-124 expression level was strong down-regulated in CCA tissues compared with normal para-cancerous tissues (P<0.001). Moreover, aberrant miR-124 expression was significantly associated with differentiation (P=0.045) and lymph node metastasis (P=0.040). In addition, Kaplan-Meier method and log-rank test revealed that patients with low miR-124 expression has a poorer overall survival compared with those with high miR-124 expression (P=0.002). Furthermore, multivariate analysis confirmed that miR-124 expression (P=0.006; HR=2.006; 95%CI: 1.224-3.289) was an independent prognostic indicator in CCA.Conclusions: Collectively, our results defined miR-124 expression plays important roles in CCA patients. MiR-124 expression might used as a valuable prognostic biomarker for patients with CCA.

Updated result on the 2.5-year follow-up of GC0301/TOP-002: Randomized phase III study of irinotecan plus S-1 (IRI-S) versus S-1 alone as first-line treatment for advanced gastric cancer (AGC)

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 4544-4544
Author(s):  
A. Tsuburaya ◽  
H. Narahara ◽  
H. Imamura ◽  
K. Hatake ◽  
H. Imamoto ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Clinical Benefit ◽  
Phase Iii ◽  
Rank Test ◽  
Diffuse Type ◽  
Chi Square ◽  
Log Rank Test ◽  
Chi Square Test ◽  
Significant Superiority

4544 Background: IRI-S had longer in median survival time (MST) than S-1 alone, and was well tolerated in previously untreated AGC, but not statistically significant. Considering 68 patients (pts) were censored, further follow-up was needed to confirm the OS with more precision (Imamura et al. ASCO-GI 2008). We now present updated results of OS and exploratory analysis with the prolonged 2.5 year follow-up data. Methods: Treatments Arm A (oral S-1 80 mg/m2/day from Day 1 to 28, q6w), or Arm B (IRI-S; oral S-1 80 mg/m2/day from Day 1 to 21 and intravenous irinotecan 80 mg/m2 on Days 1 and 15, q5w) were continued until disease progression or unacceptable toxicities were observed. The primary endpoint was to compare OS between groups. This updated result was regarded as exploratory position. Results: Although the MST of Arm A was 319 days (95%Cl: 286–395) and of Arm B was 389 days (95%Cl: 324–459), Arm B didn’t show statistically significant superiority to Arm A (log-rank test p=0.54; hazard ratio (HR) =0.93). The 1-year survival was 45.0% in Arm A and 52.0% in Arm B, and the 2-year survival was 22.5% and 18.0%, respectively. Response rate was significantly different (Arm A/B, 26.9%/41.5%; chi-square test p=0.04) in 187 patient evaluated by RECIST criteria. Time to treatment failure was also favored in Arm B (median=138 days) compared to Arm A (111 days; log-rank test p=0.16; HR=0.85). In subset analyses, two groups showed possibility of clinical benefit in Arm B. The HR of diffuse type group was 0.71 (95%Cl: 0.52–0.96), and of PS1, 2 group was 0.63 (95%Cl: 0.42–0.95). As post protocol treatment, 45.6% of Arm A patients received an irinotecan-based regimen, and the MST of them was 496 days (95%Cl: 395–573). Conclusions: IRI-S did not show statistically significant superiority to S-1 alone in OS with this follow-up data. Post protocol treatment, effective treatment after S-1 failure might have affected survival. According to exploratory analyses, IRI-S may have clinical benefit in early-term of treatment, group of the diffuse type and that of PS1, 2. We need more considering predictive factors, because the gastric cancer is heterogeneous adenocarcinoma. [Table: see text]

PCAT-1 Expression is Associated With The Prognosis in Prostate Cancer.

2020 ◽  
Author(s):  
Sheng Li ◽  
Xiaolan Ruan ◽  
Tongzu Liu
Keyword(s):  
Prostate Cancer ◽  
Cox Regression ◽  
Prognostic Biomarker ◽  
Univariate Analysis ◽  
Prognostic Significance ◽  
Survival Curves ◽  
Chi Square ◽  
Cox Regression Analysis ◽  
Chi Square Test ◽  
Cox Analysis

Abstract Purpose: In the study, we aimed to estimate the prognostic significance of PCAT-1 in patients with prostate cancer (PCa).Methods: The expression of PCAT-1 in paired PCa tissues and normal controls was examined via quantitative real-time polymerase chain reaction (qRT-PCR). The influence of PCAT-1 level on clinical features was assessed using Chi-square test. The survival curves were plotted to estimate the prognosis of patients. And the Cox analysis was carried out to find promising predictive factors for patients.Results: The expression level of PCAT-1 in PCa tissues was significantly elevated compared with the adjacent normal control (P<0.0001). The increased expression of PCAT-1 was affected by high Gleason score (P=0.017), positive serum PSA (P=0.011) and advanced TNM stage (P=0.003). The Kaplan-Meier survival curves showed that the overall survival rate of patients with high PCAT-1 expression was significantly lower than those with low PCAT-1 expression (P<0.001). Both univariate analysis (P=0.000, HR=10.623, 95%CI=5.798-19.464) and multivariate Cox regression analysis (P=0.000, HR=10.996, 95%CI=5.896-20.507) revealed that PCAT-1 could act as a prognostic biomarker for PCa patients.Conclusion: Taken together, overexpression of PCAT-1 is involved in PCa progression and could be a potential prognostic biomarker for PCa patients.

scholarly journals Connection between Gab1 Polymorphisms and cholangiocarcinoma patients prognosis

2020 ◽  
Author(s):  
Ning Wang ◽  
Yanni Li ◽  
Yanfang Zheng ◽  
Huoming Chen ◽  
Xiaolong Wen ◽  
...  
Keyword(s):  
Cox Regression ◽  
Survival Rates ◽  
Prognostic Significance ◽  
Positive Lymph Node ◽  
Rank Test ◽  
Chi Square ◽  
Cox Regression Analysis ◽  
Normal Tissues ◽  
Qrt Pcr ◽  
Chi Square Test

Abstract Background We conducted this study to measure the levels of GRB2 associated binding protein 1 (Gab1) mRNA in cholangiocarcinoma (CCA) tissues and the paired normal tissues. And then assess the prognostic significance of Gab1 in CCA patients. Methods Quantitative real-time (qRT-PCR) was adopted to confirm the expression levels of Gab1 mRNA in both CCA tissues and normal controls. The Chi-square test was applied to estimate the influence of clinical parameters on Gab1 expression. Kaplan-Meier survival analysis with log-rank test were used to determine overall survival rates of patients with different Gab1 expression. Cox regression analysis was carried out to evaluate prognostic value of factors in CCA patients. Results According to the qRT-PCR result, increased expression of Gab1 mRNA was found in CCA tissues compared with the paired noncancerous controls (P<0.0001). The Chi-square test demonstrated that the elevated expression of Gab1 was affected by poor differentiation (P=0.022), positive lymph node metastasis (P=0.008) and high TNM stage (P=0.004). However, age, gender, family history and resection margin had no significant influence on Gab1 levels (all, P>0.05). The survival curves suggested that up-regulation of Gab1 was related with poor prognosis of CCA patients. Furthermore, the Cox analysis demonstrated Gab1 was a prognostic biomarker for CCA patients (P=0.000, 95%CI=2.576, HR=1.552-4.275). Conclusion Taken together, Gab1 expression was increased in CCA and may be a useful biomarker to predict the outcomes of CCA patients.

scholarly journals Ανοσοϊστοχημική μελέτη της ογκογόνου πρωτεΐνης Υ-box binding protein-1 (Yb-1) σε διηθητικά καρκινώμτα μαστού

2014 ◽  
Author(s):  
Σάββας Μελισσάρης
Keyword(s):  
Regression Model ◽  
Binding Protein ◽  
Rank Test ◽  
Chi Square ◽  
Ki 67 ◽  
Hazard Regression ◽  
Log Rank Test ◽  
Chi Square Test ◽  
Her 2

Η πρωτεΐνη YB-1 αποτελεί έναν μεταγραφικό-μεταφραστικό παράγοντα, είναι μέλος της οικογένειας των πρωτεϊνών που δεσμεύουν DNA και RNA και περιέχει μία καλά διατηρημένη περιοχή ψυχρού σοκ που αντιδρά με ανεστραμμένα CCAAT-boxes (Y-boxes) στην περιοχή εκκινητών διαφόρων ευκαρυωτικών γονιδίων και κυρίως γονιδίων σχετιζόμενα με την αύξηση και τον πολλαπλασιασμό των κυττάρων. Η ΥΒ-1 ανευρίσκεται κυρίως στο κυτταρόπλασμα των κυττάρων ενώ ένα μικρό ποσοστό μπορεί να ανευρεθεί και στον πυρήνα κατά την G1/S φάση του κυτταρικού κύκλου ύστερα από απάντηση σε εξωκυττάρια ερεθίσματα όπως η υπερθερμία, η υπεριώδης ακτινοβολία αλλά και ύστερα από χορήγηση στα κύτταρα θρομβίνης, ιντερφερόνης και ινσουλινομιμητικού παράγοντα 1 (IGF-1).Η πρωτεΐνη ΥΒ-1, υπερεκφράζεται σε καρκινώματα διαφόρων οργάνων (π.χ. του προστάτη, του παχέος εντέρου, των ωοθηκών, των πνευμόνων, των οστών (πολλαπλό μυέλωμα), στο γλοιοβλάστωμα) καθώς και στον καρκίνο του μαστού. Ο σκοπός της παρούσας μελέτης ήταν η διερεύνηση του προτύπου έκφρασης της πρωτεΐνης ΥΒ-1 σε 225 διηθητικά καρκινώματα μαστού. Τα αποτελέσματα του ανωτέρω δείκτη υποβλήθηκαν σε στατιστική ανάλυση για τη διερεύνηση της σχέσης τους με α) Κλασικές κλινικοπαθολογοανατομικές παραμέτρους (ηλικία, εμμηνοπαυσιακή κατάσταση, μέγεθος όγκου, ιστολογικός τύπος, ιστολογικός και πυρηνικός βαθμός κακοηθείας, κατάσταση των επιχωρίων λεμφαδένων, στάδιο της νόσου, β) Νεότερους προγνωστικούς δείκτες, όπως ανοσοϊστοχημική έκφραση των ορμονικών υποδοχέων ER, PR και της ογκοπρωτεΐνης HER-2, γ) Σηματοδοτικά μόρια, όπως ανοσοϊστοχημική έκφραση των δεικτών Ki-67, p53, bcl-2, δ) Το φαινότυπο CD44+/CD24-/low, που θεωρείται ότι αντιπροσωπεύει τα καρκινικά αρχέγονα κύτταρα του μαστού και ε) Την ολική και ελευθέρα νόσου επιβίωση των ασθενών. Με βάση τις συσχετίσεις που προέκυψαν γίνεται εκτίμηση της πιθανής συμμετοχής της πρωτεΐνης ΥΒ-1 στην επιθετικότητα και πρόγνωση του καρκίνου του μαστού.Το υλικό αποτέλεσαν 225 πρωτοπαθή διηθητικά καρκινώματα μαστού από τα οποία 169 ήταν πορογενή και 50 λοβιακά, προερχόμενα από 225 θήλεις ασθενείς ηλικίας από 25 έως 87 έτη. Το μέσο διάστημα παρακολούθησης για την ολική επιβίωση των ασθενών ήταν 189 μήνες. Εξήντα πέντε ασθενείς εκδήλωσαν υποτροπές, με μέσο διάστημα παρακολούθησης 189 μήνες. Για τον προσδιορισμό της πρωτεΐνης εφαρμόστηκε η ανοσοϊστοχημική μέθοδος τριών βημάτων του συμπλέγματος αβιδίνης – βιοτίνης – υπεροξειδάσης. Η στατιστική επεξεργασία των αποτελεσμάτων και των επιμέρους συσχετίσεων έγινε με μονοπαραγοντική (chi-square test και log rank test) και πολυπαραγοντική (Cox’s hazard regression model) ανάλυση.Αποτελέσματα: Η πρωτεΐνη ΥΒ-1 εντοπίστηκε στο κυτταρόπλασμα και τον πυρήνα των καρκινικών κυττάρων σε 93 (41,3%) και 52 (23,10%) περιπτώσεις, αντίστοιχα, καθώς και στους στρωματικούς ινοβλάστες σε 123 (55%) περιπτώσεις. Η κυτταροπλασματική έκφραση της πρωτεΐνης συσχετίσθηκε με την προεμμηνοπαυσιακή κατάσταση των ασθενών (p=0,018), με διηθητικά πορογενή καρκινώματα (p=0,002), παράλληλα με τον πυρηνικό βαθμό κακοηθείας (p<0,001), ανάστροφα με την έκφραση των ER και της πρωτεΐνης Bcl-2 (p=0,002 και p=0,042, αντίστοιχα), παράλληλα με την έκφραση των πρωτεϊνών Ki-67 και p53 (p=0,022 και p=0,002, αντίστοιχα) και παράλληλα με το φαινότυπο CD44+/CD24-/low (p=0,001). Η εντόπιση της πρωτεΐνης σε αυτό το κυτταρικό διαμέρισμα δεν βρέθηκε να ασκεί κάποια επίδραση στην ολική και ελευθέρα-νόσου επιβίωση των ασθενών. Η πυρηνική έκφραση της ΥΒ-1, ενώ δεν συσχετίσθηκε μέ κάποιον από τους προγνωστικούς δείκτες του καρκίνου του μαστού ή κάποιον από τους νεότερους βιολογικούς δείκτες, βρέθηκε να ασκεί μη ευνοϊκή επίδραση στην ελευθέρα-νόσου επιβίωση: α) του συνόλου των ασθενών (p=0,05), β) των ασθενών που είχαν υποβληθεί σε επικουρική χημειοθεραπεία ή ακτινοθεραπεία (p=0,036 και p=0,05, αντίστοιχα), γ) των ασθενών με καρκινώματα λοβιακού ιστολογικού τύπου (p=0,006), δ) των ασθενών με καρκινώματα που είχαν ταυτόχρονη απουσία των ER και PR υποδοχέων (p=0,008), ε) των ασθενών με καρκινώματα θετικά για την έκφραση του υποδοχέα HER-2 (p=0,018) και στ) των ασθενών με καρκινώματα HER-2 μοριακού υπότυπου [ER(-)/PR(-)/HER-2(+)] (p=0,023). Η στρωματική έκφραση της ΥΒ-1 δεν συσχετίσθηκε με κάποια από τις κλινικοπαθολογοανατομικές παραμέτρους του καρκίνου του μαστού ή την επιβίωση των ασθενών. Ωστόσο, βρέθηκε να σχετίζεται παράλληλα με την έκφραση των πρωτεϊνών HER-2 και p53 (p=0,011 και p=0,021, αντίστοιχα) και ανάστροφα με την έκφραση της πρωτεΐνης Bcl-2 (p=0,018). Συμπερασματικά, τα αποτελέσματα της πρωτεΐνης υποδηλώνουν ότι η κυτταροπλασματική εντόπιση της ΥΒ-1 σχετίζεται με πιο επιθετικό φαινότυπο του καρκίνου του μαστού. Η πυρηνική εντόπιση της πρωτεΐνης, συσχετίστηκε με χειρότερη ελευθέρα-νόσου επιβίωση στο σύνολο των ασθενών. Επίσης, η αρνητική συσχέτιση της πυρηνικής ΥΒ-1 με την ελευθέρα-νόσου επιβίωση των ασθενών που είχαν λάβει επικουρική ακτινοθεραπεία ή χημειοθεραπεία, αναδεικνύει τη σημασία της στην απόκτηση αντίστασης σε διάφορα θεραπευτικά σχήματα που χρησιμοποιούνται για την αντιμετώπιση του καρκίνου του μαστού και την καθιστά ως ένα πιθανό προγνωστικό-προβλεπτικό δείκτη.

Prognostic value of piR-39980 in patients with colorectal cancer.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e16087-e16087
Author(s):  
Wei Peng ◽  
Jingfeng Mei ◽  
Jianwei Lu ◽  
Jun Bao ◽  
Guoren Zhou
Keyword(s):  
Colorectal Cancer ◽  
Overall Survival ◽  
Prognostic Value ◽  
Cox Regression ◽  
Survival Curve ◽  
Prognostic Significance ◽  
Rank Test ◽  
Chi Square ◽  
Log Rank Test ◽  
Independent Indicator

e16087 Background: Colorectal cancer (CRC) accounts for a common gastrointestinal malignancy all over world. Piwi-interacting RNAs (piRNAs) show a substantial role in the oncogenesis of a variety of tumors. The objective of this work was to uncover the expression profile of piR-39980 and its prognostic value in CRC. Methods: The levels of piR-39980 expression in CRC tissues and paired normal tissues was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Association of piR-39980 with CRC clinical feathers was assessed by Chi-square test. Overall survival curve was built via log-rank test by Kaplan–Meier analysis. The prognostic significance of piR-39980 in CRC was measured by Cox regression model. Results: piR-39980 was upregulated in CRC specimens than the paired normal specimens ( P < 0.001). Importantly, upregulation of piR-39980 was related to tumor size and T stage (all P < 0.05). Survival evaluation suggested that CRC folks with high expression of piR-39980 went through poorer overall survival than folks with low piR-39980 expression (log rank test, P = 0.0429). piR-39980 could be an independent indicator for CRC patients’ prognosis (HR = 3.308, 95% CI = 1.762-6.594, P = 0.043). Conclusions: piR-39980 plays oncogenic roles in CRC tumorigenesis and may be an independent indicator for CRC prognosis.

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