scholarly journals Evaluation of The Effect of Epiphytic, Endophytic And Rhizosphere Bacteria On Seed Germination And Seedling Characteristics

2021 ◽  
Author(s):  
Mohammad Razinataj ◽  
Gholam Khodakaramian
Keyword(s):  
Seed Treatment ◽  
Bacillus Pumilus ◽  
Germination Rate ◽  
Rhizospheric Bacteria ◽  
Root And Shoot Length ◽  
Cotton Plants ◽  
Golestan Province ◽  
Shoot Weight ◽  
Phenotypic Tests ◽  
Seedling Characteristics

Abstract During 2013 and 2014, in order to investigate the effect of bacteria on the characteristics and growth rate of cotton seedlings, isolated epiphytic, endophytic and rhizospheric bacteria from cotton plants in Golestan province. Based on biochemical and phenotypic tests, amplification and sequencing of 16S rRNA gene, were identified strains. Isolates of Bacillus pumilus MR11, B. pumilus MR12, B. pumilus MR13, B. safensis MR21, B. safensis MR22 and Stenotrophomonas pavanii MR31 identified as superior endophytes and rhizosphere. Also Pseudomonas fluorescens, P. syringae and Pantoea annanatis were identified as epiphytic from plants and seeds. These isolates were evaluated to effect on seedling growth characteristics. Based on results, the six-day-old seedlings treated with Bacillus pumilus MR11 and B. pumilus MR12 had the highest root and shoot length and fresh shoot weight. Due to the fact that B. pumilus MR12 and S. pavanii MR31 isolates had the highest percentage and germination rate, so their use as seed treatment can increase the percentage and germination rate of seeds and be effective in reducing the damage caused by seedling diseases. This is the first report of isolation of B. safensis, B. pumilus and S. pavanii bacteria from rhizosphere and cotton endophytes in Iran.

scholarly journals Physiological response of cotton seeds treated with thiamethoxam under heat stress

2016 ◽  
Vol 38 (2) ◽  
pp. 140-147 ◽  
Author(s):  
Luciana Regina Lauxen ◽  
Andréia da Silva Almeida ◽  
Cristiane Deuner ◽  
Géri Eduardo Meneghello ◽  
Francisco Amaral Villela
Keyword(s):  
Low Temperature ◽  
Chlorophyll Content ◽  
Water Availability ◽  
Seed Treatment ◽  
Shoot Length ◽  
Root And Shoot Length ◽  
Cotton Plants ◽  
Emergence Percentage ◽  
Cotton Seeds

Abstract: This study aimed to verify the effect of seeds treated with thiamethoxam on the emergence, growth and chlorophyll content of seedlings of two cotton cultivars subjected to abiotic stress. The seeds used were from the DeltaOpal and NuOpal cultivars, each one represented by three lots, previously subjected to determination of moisture content, germination and vigor (low temperature germination). Then, the seeds from each lot, treated with thiamethoxam at a dose of 600 mL of product per 100 kg-1 seed or not, were exposed to temperature (18, 25 and 35 °C) and water availability (40, 60 and 80%) stress conditions. The emergence percentage, the root and shoot length, and the chlorophyll content were evaluated. The growth of cotton seedlings from the DeltaOpal and NuOpal cultivars, subjected to low temperature conditions and water availability of 40 to 60%, is favored by seed treatment with thiamethoxam. Seed treatment with thiamethoxam increases chlorophyll content during the development of cotton plants.

scholarly journals Effect of Oak Tree Sawdust Fermentation Period on Peanut Seed Germination, Seedling Biomass, and Morphology

Horticulturae ◽  
2021 ◽  
Vol 7 (7) ◽  
pp. 182
Author(s):  
Junsik Ahn ◽  
Soyeon Oh ◽  
Yang Joo Kang ◽  
KiBum Kim ◽  
Sung-Kwon Moon ◽  
...  
Keyword(s):  
Microbial Community ◽  
Seed Germination ◽  
Germination Rate ◽  
Peanut Seed ◽  
Arachis Hypogaea L ◽  
Vigor Index ◽  
Seedling Biomass ◽  
Oak Tree ◽  
Fermentation Period ◽  
Seedling Characteristics

Peanut (Arachis hypogaea L.) seeds were germinated to investigate the effect of the fermentation period of oak tree sawdust on germination viability and seedling characteristics. Its germination rate, seedling weight, length, and total vigor index were assessed. The seeds were sown in oak tree sawdust fermented for 0, 30, 45, and 60 days. The germination rates of the seeds in fermented sawdust were significantly different. The seeds in the 45-day fermented sawdust produced the heaviest biomass weight (4.6 g) with the longest true leaf (1.7 cm) and hypocotyl (3.4 cm) resulting in the highest total vigor index (925.8). In contrast, seeds in 0-day fermented sawdust had the lowest total vigor index (18.3). Microbiome analysis showed that the microbial community in the sawdust changed as the fermentation progressed, indicating that the microbial community seems to affect seed germination physiology. Taken together, 45-day fermented sawdust is recommended for optimal peanut seed germination and seedling growth.

scholarly journals Raw and Fermented Alfalfa Brown Juice Induces Changes in the Germination and Development of French Marigold (Tagetes patula L.) Plants

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1076
Author(s):  
Döme Barna ◽  
Szilvia Kisvarga ◽  
Szilvia Kovács ◽  
Gábor Csatári ◽  
Ibolya O. Tóth ◽  
...  
Keyword(s):  
Seed Germination ◽  
Tap Water ◽  
Germination Rate ◽  
Ornamental Plants ◽  
Dry Mass ◽  
Water Soluble ◽  
Tagetes Patula ◽  
Root And Shoot Length ◽  
Horticultural Practice ◽  
Brown Juice

Organic and ecological farming programs require new and efficient biostimulants with beneficial properties for the sustainable and safe production of seedlings and ornamental plants. We examined the effect of non-fermented and lacto-fermented alfalfa brown juice (BJ) on seed germination and the vegetative, physiological, and anatomical properties of French marigold (Tagetes patula L. ‘Csemő’) plants which were treated with 0.5–10% fermented and non-fermented BJ, with tap water applied as a control. Applying 0.5% fermented BJ significantly improved seed germination compared with non-fermented BJ, resulting in an increase of 9.6, 11.2, 10.9, and 41.7% in the final germination percent, germination rate index, germination index, and vigor index, respectively. In addition, it increased the root and shoot length by 7.9 and 16.1%, respectively, root and shoot dry mass by 20 and 47.6%, respectively, and the number of leaves by 28.8% compared to the control. Furthermore, an increase in contents of water-soluble phenol, chlorophyll a and b, and carotenoid was reported upon the application of 0.5% fermented BJ, while peroxidase activity decreased. Our results prove that alfalfa BJ can be enrolled as a biostimulant as part of the circular farming approach which supports the sustainable horticultural practice.

scholarly journals Impacts of different salt source and concentrations on germination and seedling growth of many pumpkin seeds used as rootstoch in Iran

Genetika ◽  
2012 ◽  
Vol 44 (2) ◽  
pp. 235-250 ◽  
Author(s):  
Ahmad Dadashpour
Keyword(s):  
Electrical Conductivity ◽  
Seed Germination ◽  
Seedling Growth ◽  
Root Length ◽  
Germination Rate ◽  
Shoot Length ◽  
Root Weight ◽  
Pumpkin Seeds ◽  
High Concentrations ◽  
Shoot Weight

The effects of different salt sources (C Cl2, NaCl, and KCl) and concentrations, as measured by electrical conductivity, (0, "control", 1, 3, 5, 7 and 9 dS m-1) on seed germination and seedling growth of ?Ferro?, ?Obez?, ?RS 841? and ?Strong Tosa F1? pumpkin varieties used as rootstock were investigated in this study. The results showed that germination rate, root length, shoot length, fresh root weight, dry root weight, fresh shoot weight and dry shoot weights tend to decrease when the electrical conductivity of the solution is higher than 5 dS m-1, independent of salt sources and in all of the varieties. Three days after seeding, a germination ratio of 5 % was obtained from RS 841 variety in all salt source and concentrations, while a germination ratio over 50 % was obtained in ?Strong Tosa? variety for the same conditions except CaCl2 salt source. Nevertheless, seeds germinated in medium having high concentrations of CaCl2 had lower germination rate and poor seedling growth, compared to media having the same concentrations of NaCl and KCl. It was concluded that all of the varieties studied were more sensitive to the concentrations prepared using CaCl2 than that of the KCl, and NaCl.

Survival and colonization of rhizobacteria in a tomato transplant system

2003 ◽  
Vol 49 (6) ◽  
pp. 383-389 ◽  
Author(s):  
Zhinong Yan ◽  
M S Reddy ◽  
Joseph W Kloepper
Keyword(s):  
Plant Growth ◽  
Pseudomonas Fluorescens ◽  
Seed Treatment ◽  
Bacillus Pumilus ◽  
Growth Promotion ◽  
Lateral Roots ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Colonization ◽  
Initial Inoculum ◽  
Application Method

Plant-growth-promoting rhizobacteria (PGPR) are used on crops most often as seed treatments; however, an alternative application method for transplanted vegetables is mixing PGPR into the soilless medium in which the transplants are grown. Studies were undertaken to compare root colonization and persistence of rifampicin-resistant mutants of PGPR strains Bacillus pumilus SE34 and Pseudomonas fluorescens 89B61, SE34r and 89B61r, on tomato as a function of application method. When the bacteria were incorporated into Promix(tm) soilless medium at log 6, 7, and 8 colony- forming units/g, populations of strain SE34r per gram of medium maintained the initial inoculum densities, while populations of 89B61r decreased approximately one to two orders of magnitude by 4 weeks after planting. The populations of each PGPR strain colonizing roots after application into the soilless medium showed a similar pattern at 6 weeks as that at 4 weeks after planting, with higher populations on the whole roots and lateral roots than on the taproots. Strain SE34r but not 89B61r moved upwards and colonized the phyllosphere when incorporated into the soilless medium. Following application as seed treatment, populations of SE34r were significantly higher on upper roots and on the taproot than were populations following application through the soilless medium. Conversely, populations were higher on lower roots and lateral roots following application through the soilless medium than were populations following application as seed treatment. While strain SE34 enhanced plant growth with application both to the medium and as seed treatment, the level of growth promotion was significantly greater with application in the soilless medium. The results indicate that PGPR can be successfully incorporated into soilless media in vegetable transplant production systems.Key words: rhizobacteria, plant colonization, Bacillus pumilus, Pseudomonas fluorescens.

scholarly journals Effect of Salinity and Drought Stress on Germination Indices of Two Thymus Species

2012 ◽  
Vol 45 (1) ◽  
pp. 27-35 ◽  
Author(s):  
F. Khoshsokhan ◽  
M. Babalar ◽  
H. Chaghazardi ◽  
M. Moghadam
Keyword(s):  
Drought Stress ◽  
Germination Rate ◽  
Germination Percentage ◽  
Relative Resistance ◽  
Field Crops ◽  
Root And Shoot Length ◽  
Medical Plants ◽  
West Azerbaijan ◽  
Germination Indices ◽  
Arid And Semiarid

Effect of Salinity and Drought Stress on Germination Indices of Two Thymus SpeciesThe environmental stress such as, salinity (soil or water) and drought are serious obstacles for medical plants and field crops in further areas of the world, especially arid and semiarid regions. In order to investigate salinity and drought stress onT. daenensisand T.kotschyanusgermination indices, an experiment was carried out. To create salinity stress, sodium chloride (NaCl) at the levels of 0 (as control), -3, -6 and -9 bar, and for drought stress, polyetylenglycol 6000 (PEG 6000) in osmotic levels at 0 (as control), -3, -6 and -9 bar were used. Effect of drought and salt levels on investigated traits significant at 0.01. Result showed significance different between evaluated indices. Increasing of stress levels lead to reduction of germination and root and shoot length. Salt stress only in high levels reduced germination rate. Therefore,T. daenensisandT. kotschyanushave relative resistance to salinity in germination stage. Decrease in osmotic potential progressively inhibited seed germination of the two thyme species. In all salt treatments, maximum germination percentage inT. kotschyanusrelated to West Azerbaijan population and maximum germination rate related to Gazvin population. But in T. daenensis the highest values (both rate and percentage germination) were observed in Arak population. The germination rate of T.daenensisto the drought stress was more than theT. kotschyanusgermination rate like the germination percentage. Comparing the two stresses, it appears that drought has more effect than salt in germination indices.

scholarly journals Influence of presowing treatment of seeds by growth regulators and fungicides on field similarity of Zinnia elegans

2020 ◽  
pp. 44-49
Author(s):  
Nikolay Vasilievich Smolin ◽  
Yulia Nikolaevna Nedaiborshch ◽  
Natalya Vasilievna Potapova ◽  
Andrey Sergeevich Saveliev ◽  
Victor Vladimirovich Volgin ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Seed Treatment ◽  
Germination Rate ◽  
Stress Factors ◽  
Juvenile Period ◽  
Presowing Treatment ◽  
Stress Components ◽  
Rate Of Emergence ◽  
The One ◽  
Positive Effect

The article discusses the effect of presowing seed treatment by growth regulators and fungicides on the field germination of seeds of two grades of zinnia elegant – Dream and Orange King. Varieties differed in seed germination time. On average, over three years, Zinnia Dream seeds germinated 0.5–1.0 days earlier than Orange King seeds. The treatment of zinnia seeds with an epin-extra growth regulator contributed to the emergence of earlier seedlings. When the seeds were soaked with a solution of this preparation, the plants went through the sprouting phase faster – the period when they are most sensitive to abiotic stresses. Zircon had a weaker effect on the germination rate of zinnia seeds than epin-extra, however, the presence of a prolonged action of this drug in the activation of non-specific resistance of plants to stress factors can also be noted. The one-component disinfectant benorad turned out to be the most passive and did not significantly affect the germination of zinnia. There were no visible signs of manifestation of pathogens in the juvenile period of plants, and its active substance (benomyl) could hardly affect the rate of emergence of seedlings. In contrast to benorad, the two-component protectant vial TrasT had a weak positive effect on the rate of emergence of seedlings, since it included specially introduced anti-stress components.

scholarly journals Temperature and Relative Humidity Govern Germination and Storage of Gladiolus Seed

HortScience ◽  
1991 ◽  
Vol 26 (8) ◽  
pp. 1054-1057 ◽  
Author(s):  
W.J. Carpenter ◽  
G.J. Wilfret ◽  
J.A. Cornell
Keyword(s):  
Relative Humidity ◽  
Seed Treatment ◽  
No Reduction ◽  
Germination Rate ◽  
Seed Viability ◽  
Seed Storage ◽  
Moisture Contents ◽  
Seed Moisture ◽  
Germination Temperature ◽  
And Storage

Gladiolus (G. grandiflorus) seed germination was light-independent, but temperature influenced the germination rate. Constant 20C promoted higher total germination (97%), fewer days (4.3) to 50% of final germination, and shorter span of days (4.8) between 10% and 90% germination than other constant temperatures, although similar results were achieved by alternating 12-h cycles of 20 to 25C. Total germination was unchanged after seed treatment for 7 days at 10 to -20C, but longer germination periods were required after treatments below -10C. Reducing seed moisture contents from 11.8% to 4.2% caused no reduction in total germination, but moisture contents below 6.6% delayed achieving 50% of final germination and extended the periods from 10% to 90% of germination. Temperature and relative humidity (RH) during storage were important in retaining seed viability, with RH having a larger effect. Smallest declines in total germination during 12 months of storage occurred at 11% and 33% RH at 15C. The statistical analysis estimated the optimum seed storage at 14C and 26% RH.

scholarly journals The Effect of Osmopriming on Seed Germination and Early Seedling Characteristics of Carum carvi L.

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 94
Author(s):  
Iman Mirmazloum ◽  
Attila Kiss ◽  
Éva Erdélyi ◽  
Márta Ladányi ◽  
Éva Zámboriné Németh ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Germination ◽  
Germination Rate ◽  
Petri Dish ◽  
Seed Priming ◽  
Germination Percentage ◽  
Positive Effects ◽  
Carum Carvi ◽  
Early Seedling ◽  
Seedling Characteristics

Two experiments (in Petri dishes and in soil) were conducted to investigate the effects of osmopriming on seed germination and the early seedling characteristics of caraway (Carum carvi L. var. annua). The priming treatments in the Petri dish experiment were: polyethylene glycol (5%, 10% and 20%), KNO3 (0.5%, 1% and 2%) and KCL (1%, 2% and 4%) with three different soaking times (12, 24 and 36 h) along with control (non-primed seeds). Only polyethylene glycol and H2O were applied in the cell tray experiment, which were then compared with the non-primed seeds. In the Petri dish experiment, all three priming reagents significantly enhanced seedling length, with the most effective treatments being 5% PEG, 2% KNO3 and 1% KCL for 12 h. The plumule dry weights were also increased significantly after PEG (20% for 36 h), KNO3 (2% for 24 and 36 h) and KCL (1% for 12 h) treatments in comparison with the control. In the soil experiment, osmopriming with PEG significantly improved the germination rate (GR) and percentage, the plumule dry and fresh weights and the plumule length of caraway seedlings when compared with the control. A 23% higher germination percentage was recorded for the seeds treated with 5% PEG for 24 h as compared with the non-primed seeds. The PEG-primed seeds produced significantly longer seedlings when treated with 5% PEG for 24 h. All of the applied PEG treatments significantly enhanced the plumule fresh and dry weights, with the best outcomes being after 5% PEG (24 h) and 10% PEG (36 h) treatments, respectively. The 12-h hydro-priming also significantly enhanced all of the studied germination parameters when compared to the control. The results of the presented experiments show the significant positive effects of seed priming on caraway germination and how early seedling performance can easily be adopted by producers.

scholarly journals Effect of various biological control agents (BCAs) on drought resistance and spring barley productivity

2020 ◽  
Vol 17 ◽  
pp. 00063
Author(s):  
Radik Safin ◽  
Lilia Karimova ◽  
Lubov Nizhegorodtseva ◽  
Daria Stepankova ◽  
Gulnaz Shaimullina ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Seed Treatment ◽  
Spring Barley ◽  
Trichoderma Viride ◽  
Biological Agents ◽  
Field Conditions ◽  
Streptomyces Sp ◽  
Rhizospheric Bacteria ◽  
Barley Resistance

The aim of the article is to study the effect of various biological agents of biofungicides on plant resistance to drought and formation of spring barley crops. Endophytic bacteria (Bacillus subtilis), rhizospheric bacteria (Pseudomonas fluorescens), actinomycetes (Streptomyces sp.), and micromycetes (Trichoderma viride) were used as bioagents of biofungicides. Preparations based on biological agents were used to treat spring barley seeds. The studies were carried out in laboratory and field conditions. In the conditions of the model artificial acute drought, it was found that seed treatment with Streptomyces sp. Stimulates the leaf and root growth, as well as increases peroxidase activity in the leaves. The maximum content of proline was during seed treatment with Pseudomonas fluorescens. An increase in the content of chlorophyll was observed when treating with Trichoderma viride seeds. In the field conditions, Pseudomonas putida and Streptomyces were more efficient by the content of proline in seedlings. In terms of the spring barley yield, seed treatment with Streptomyces sp., Pseudomonas putida and Bacillus subtilis was more efficient. Streptomyces sp., Pseudomonas putida and Bacillus subtilis are promising for increasing barley resistance to drought.

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