scholarly journals Contribution of Autologous Omentum Transposition to the Regeneration of Renal Injuries in the Rat Model

2020 ◽  
Author(s):  
Tayfun Bilgic ◽  
Umit Ince ◽  
Fehmi Narter
Keyword(s):  
Rat Model ◽  
Primary Repair ◽  
Healing Process ◽  
Histological Finding ◽  
Kidney Injury ◽  
Study Groups ◽  
Renal Tissue ◽  
Albino Rats ◽  
Loss Of Function ◽  
Renal Injuries

Abstract Aim: After renal trauma, surgical treatment is vital, but sometimes there may be loss of function due to fibrosis. We aimed to evaluate the repair effect of transpositioned autologous omentum on injured renal tissue in a rat model. Methods: A total of 30 female Wistar Albino rats were included and they were randomly separated into a sham group and four study groups. Iatrogenic renal injuries were repaired using a surgical technique (primary repair 1 and 2 groups) or transpositioned autologous omentum (omentum repair 1 and 2 groups). In all groups, blood samples were taken preoperatively and on the 7 th postoperative day in all groups and also on the 18 th postoperative day in the control and two study groups. All rats were sacrificed on the 7 th or 18 th day postoperatively and their right kidneys were taken for histopathological evaluation.Results: There was a trend toward decrease in urea and creatinine levels in all the groups. There was no significant correlation between urea and creatinine levels and histological finding scores. The omentum repair group had significantly lower inflammation and granulation scores compared with the primary repair and sham groups. There was a significant and positive correlation between inflammation and granulation and fibrosis scores. There was a significant and negative correlation between healing completion score and either inflammation and granulation scores. There were also positive correlations between histological findings in the kidney specimen and surrounding tissues. Conclusion: The use of the autologous omentum tissue for repair of kidney injury had attenuation effects on inflammation and granulation compared with primary repair. These results imply that use of omentum tissue to facilitate healing of kidney injury may theoretically lead to a more effective healing process and reduced fibrosis and tissue and function loss. These potentially beneficial effects of autologous omentum tissue should be investigated in further well-designed experimental and clinical studies.

scholarly journals Contribution of Autologous Omentum Transposition to the Regeneration of Renal Injuries in the Rat Model

2020 ◽  
Author(s):  
Tayfun Bilgic ◽  
Umit Ince ◽  
Fehmi Narter
Keyword(s):  
Rat Model ◽  
Primary Repair ◽  
Healing Process ◽  
Histological Finding ◽  
Kidney Injury ◽  
Study Groups ◽  
Renal Tissue ◽  
Albino Rats ◽  
Loss Of Function ◽  
Renal Injuries

Abstract Aim: After renal trauma, surgical treatment is vital, but sometimes there may be loss of function due to fibrosis. We aimed to evaluate the repair effect of transpositioned autologous omentum on injured renal tissue in a rat model.Methods: A total of 30 female Wistar Albino rats were included and they were randomly separated into a sham group and four study groups. Iatrogenic renal injuries were repaired using a surgical technique (primary repair 1 and 2 groups) or transpositioned autologous omentum (omentum repair 1 and 2 groups). In all groups, blood samples were taken preoperatively and on the 7th postoperative day in all groups and also on the 18th postoperative day in the control and two study groups. All rats were sacrificed on the 7th or 18th day postoperatively and their right kidneys were taken for histopathological evaluation. Results: There was a trend toward decrease in urea and creatinine levels in all the groups. There was no significant correlation between urea and creatinine levels and histological finding scores. The omentum repair group had significantly lower inflammation and granulation scores compared with the primary repair and sham groups. There was a significant and positive correlation between inflammation and granulation and fibrosis scores. There was a significant and negative correlation between healing completion score and either inflammation and granulation scores. There were also positive correlations between histological findings in the kidney specimen and surrounding tissues. Conclusion: The use of the autologous omentum tissue for repair of kidney injury had attenuation effects on inflammation and granulation compared with primary repair. These results imply that use of omentum tissue to facilitate healing of kidney injury may theoretically lead to a more effective healing process and reduced fibrosis and tissue and function loss. These potentially beneficial effects of autologous omentum tissue should be investigated in further well-designed experimental and clinical studies.

scholarly journals Autologous omentum transposition for regeneration of a renal injury model in rats

2022 ◽  
Vol 9 (1) ◽  
Author(s):  
Tayfun Bilgiç ◽  
Ümit İnce ◽  
Fehmi Narter
Keyword(s):  
Foreign Body ◽  
Foreign Body Reaction ◽  
Primary Repair ◽  
Healing Process ◽  
Kidney Injury ◽  
Control Group ◽  
Albino Rats ◽  
Loss Of Function ◽  
Omentum Transposition ◽  
Process Completion

Abstract Background After renal trauma, surgical treatment is vital, but sometimes there may be loss of function due to fibrosis. This study aimed to evaluate the effect of autologous omentum flaps on injured renal tissues in a rat model. Methods A total of 30 Wistar albino rats were included and randomly divided equally into a control group and four intervention groups. Iatrogenic renal injuries were repaired using a surgical technique (primary repair 1 group and primary repair 2 group) or transposition of the autologous omentum (omentum repair 1 group and omentum repair 2 group). Blood samples were taken preoperatively and on the 1st and 7th postoperative days in all groups and on the 18th postoperative day in the control and two intervention groups. All rats were sacrificed on the 7th or 18th day postoperatively, and their right kidneys were taken for histopathological evaluation. Results The mean urea level significantly decreased from day 1 to day 7 and from day 1 to day 18 in the omentum repair 2 group (P = 0.005 and P = 0.004, respectively). There were no other significant changes in urea or creatinine levels within the intervention groups (P > 0.05). There was no significant correlation between the urea and creatinine levels and the histological scores (P > 0.05). The primary repair 1 and 2 groups had significantly higher median granulation and inflammation scores in the kidney specimen than the control and omentum repair groups (P < 0.05). The omentum repair 2 group had significantly lower median granulation and inflammation scores in the surrounding tissues than the primary repair 2 group (P < 0.05). The completion score for the healing process in the kidney specimen was significantly higher in the omentum repair groups than in the primary repair groups (P < 0.05). The omentum repair 2 group had significantly lower median granulation and inflammation scores in the surrounding tissues than the primary repair 2 group (P < 0.05). Granulation degree in the kidney specimen was strongly and positively correlated with the inflammation degree (r = 0.824, P < 0.001) and foreign body reaction in the kidney specimen (r = 0.872, P < 0.001) and a strong and negative correlation with the healing process completion score in the kidney (r = − 0.627, P = 0.001). Inflammation degree in the kidney specimen was strongly and positively correlated with the foreign body reaction in the kidney specimen (r = 0.731, P = 0.001) and strongly and negatively correlated with the healing process completion score in the kidney specimen (r = − 0.608, P = 0.002). Conclusion Autologous omentum tissue for kidney injury repair attenuated inflammation and granulation. Additionally, the use of omental tissue to facilitate healing of kidney injury may theoretically lead to a more effective healing process and reduced fibrosis and tissue and function loss.

Troxerutin down-regulates KIM-1, modulates p38 MAPK signaling, and enhances renal regenerative capacity in a rat model of gentamycin-induced acute kidney injury

2018 ◽  
Vol 9 (12) ◽  
pp. 6632-6642 ◽  
Author(s):  
Samir A. Salama ◽  
Hany H. Arab ◽  
Ibrahim A. Maghrabi
Keyword(s):  
Acute Kidney Injury ◽  
Renal Function ◽  
P38 Mapk ◽  
Tissue Regeneration ◽  
Rat Model ◽  
Tissue Injury ◽  
Kidney Injury ◽  
Renal Tissue ◽  
Mapk Signaling ◽  
Regenerative Capacity

Troxerutin enhances renal tissue regeneration, improves renal function, and decreases renal tissue injury in gentamycin-treated rats.

scholarly journals The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Ender Hur ◽  
Alev Garip ◽  
Asuman Camyar ◽  
Sibel Ilgun ◽  
Melih Ozisik ◽  
...  
Keyword(s):  
Vitamin D ◽  
Acute Kidney Injury ◽  
Rat Model ◽  
Urine Volume ◽  
Kidney Injury ◽  
Control Group ◽  
Albino Rats ◽  
Tubular Injury ◽  
Renal Histology ◽  
Experimental Rat Model

Introduction. Acute kidney injury (AKI) pathogenesis is complex. Findings of gentamicin nephrotoxicity are seen in 30% of the AKI patients. Vitamin D has proven to be effective on renin expression, inflammatory response, oxidative stress, apoptosis, and atherosclerosis. We aimed to investigate the effect of vitamin D in an experimental rat model of gentamicin-induced AKI.Methods. Thirty nonuremic Wistar albino rats were divided into 3 groups: Control group, 1 mL saline intramuscular (im) daily; Genta group, gentamicin 100 mg/kg/day (im); Genta + vitamin D, gentamicin 100 mg/kg/day (im) in addition to 1α, 25 (OH)2D30.4 mcg/kg/day subcutaneously for 8 days. Blood pressures and 24-hour urine were measured. Blood urea and creatinine levels and urine tubular injury markers were measured. Renal histology was semiquantitatively assessed.Results. Urea, creatinine and urine neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1 were all increased in Genta group indicating AKI model. Systolic blood pressure decreased, but urine volume and glutathione increased in Genta + Vit D group compared to Control group. Histological scores indicating tubular injury increased in Genta and Genta + Vit D groups.Conclusions. Vitamin D does not seem to be effective on histological findings although it has some beneficial effects via RAS system and a promising effect on antioxidant system.

scholarly journals The effects of mometasone furoate and strontium chloride in a rat model of allergic rhinitis

2018 ◽  
Vol 97 (1-2) ◽  
pp. E1-E7
Author(s):  
Emine Elif Altuntaş ◽  
Ömer Tamer Doğan ◽  
Bülent Saraç ◽  
Nergiz Hacer Turgut ◽  
Kasım Durmuş ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Rat Model ◽  
Study Groups ◽  
Nerve Fibers ◽  
Mometasone Furoate ◽  
Albino Rats ◽  
Strontium Chloride ◽  
Histamine Dihydrochloride ◽  
Pharmacologic Treatments ◽  
Wistar Albino Rats

Neurogenic inflammation plays a role in the pathophysiology of allergic rhinitis. Highly effective in reducing the sensory irritation caused by some substances, strontium salts directly affect C-type nerve fibers. The aim of this study was to compare the efficacy of mometasone furoate and strontium chloride on early-phase symptoms in a rat model of allergic rhinitis. Wistar albino rats (n = 24) were randomly divided into three groups: the mometasone group, receiving 1 μg mometasone furoate (2 µl/site); the strontium 3% group, receiving 3% strontium chloride (2 μl/site); and the strontium 5% group, receiving 5% strontium chloride (2 μl/site). To induce significant nasal symptoms of allergic rhinitis, 5 µmol of histamine dihydrochloride (HDC) (2 µl/site) was administered. Symptoms of allergic rhinitis were recorded as frequencies of sneezing and nasal rubbing during a 15-minute interval. On days 1 and 2, respectively, 0.9% sodium chloride (NaCl) (2 μl/site to each nasal cavity) and HDC were administered in all of the study groups. On days 3 and 4, the study drugs were administered 10 and 30 minutes before the administration of HDC. On day 5, the study drugs were administered 10 minutes after the administration of HDC. The results of the present study revealed that when strontium chloride or mometasone furoate was administered 30 minutes before the onset of symptoms, a significant decrease was observed in sneezing and nasal rubbing. The number of sneezing occurrences was significantly lower and the number of nasal rubbing occurrences was higher in the strontium 3% group compared to the groups in which mometasone furoate and 5% strontium chloride were administered after onset of symptoms. Recent studies have investigated the efficacy and safety of strontium chloride nasal drops compared with common pharmacologic treatments of allergic rhinitis. These studies have revealed that allergic rhinitis can be successfully and safely treated with strontium-chloride-containing products, thus offering a potential new treatment strategy.

scholarly journals Contribution of Autologous Omentum Transposition to the Regeneration of Renal Injuries in the Rat Model

2020 ◽  
Author(s):  
Tayfun Bilgic ◽  
Umit Ince ◽  
Fehmi Narter
Keyword(s):  
Foreign Body ◽  
Rat Model ◽  
Foreign Body Reaction ◽  
Surrounding Tissue ◽  
Control Group ◽  
Albino Rats ◽  
Histopathological Analysis ◽  
Kidney Surgery ◽  
Omentum Transposition ◽  
Renal Injuries

Abstract Aim We aimed to evaluate the repair effect of transpositioned autologous omentum on the injured renal tissues in the rat model. Methods A total of 30 female Wistar Albino rats were included and they were randomly separated into control and four groups. In all groups, blood samples were taken at preoperatively and at the 7th day postoperatively, additionally they were taken at 18th day postoperatively for creatinine and urea analysis. All rats of control group were sacrificed at 18th day postoperatively and right kidneys were taken for histopathological evaluation. After the created renal injuries were repaired primarily kidney surgery or omentum added kidney surgery. Results According to the our results, granulation and inflammation in kidney specimens were moderately and highly positive correlated with granulation, inflammation, fibrosis and foreign body reaction in surrounding tissue ((r:0.478, p = 0.008), (r:0.591, p = 0.001), (r:0.394, p = 0.031), (r:0.635, p < 0.001)). Granulation in kidney specimen was highly possitive correlated with inflammation and foreign body reaction in kidney specimens ((r:0.824, p < 0.001), (r:0.872, p < 0.001)). We could not show any significant correlation between biochemical values (U, Cr) and histopathological findings. Conclusion According to our results, our biochemical results were not correlated with histopathological analysis. But we detected histopathogical significant differences between primary kidney repair and omentum added kidney repair groups. And we recommend that kidney injuries should be surgical repaired by stem cell sources as like omentum tissue.

scholarly journals Protective Effects of Alcoholic and Aqueous Extracts of Lyophilized Kiwifruit on Acute Kidney Injury (AKI) Induced by Glycerol in Male Albino Rat

2021 ◽  
pp. 23-33
Author(s):  
Rawiyah M. Hakami ◽  
Fares K. Khalifa ◽  
Asmaa A. Alharbi
Keyword(s):  
Acute Kidney Injury ◽  
Histological Examination ◽  
Kidney Injury ◽  
Renal Tissue ◽  
Negative Control ◽  
Control Group ◽  
Albino Rats ◽  
Aqueous Extracts ◽  
Protective Effects ◽  
Albino Rat

Objectives: The study aimed to evaluate and compare the protective effects of alcoholic and aqueous extracts of lyophilized kiwifruit on acute kidney injury (AKI) induced by glycerol in male albino rats. Methods: Rats were divided into four groups: negative control healthy group, acute kidney injury group (administered glycerol), AKI rats treated with ethanolic kiwifruit extract group, and AKI rats treated with aqueous kiwifruit extract group. Kidney biomarkers, oxidative stress, and inflammatory markers were measured and histological examination was performed. Results: We observed that glycerol induced acute renal injury and as a result there was an increase in the levels of blood urea nitrogen, creatinine, Na+, K+, Cl-, uric acid, cystatin-C, neutrophil gelatinase-associated lipocalin, C-reactive protein, tumour necrosis factor-α, interleukin-6, and malondialdehyde as well as a decrease in the levels of reduced glutathione and superoxide dismutase compared to that in the negative control group. Ethanolic and aqueous kiwifruit extracts improved all the kidney biomarkers in AKI rats. Histological examination showed acute tubular necrosis in the AKI group.  However, there was an improvement in the renal tissue, represented by epithelial cell regeneration, in rats that were administered both the extracts. Conclusion: Kiwifruit extracts have a positive effect on serum biochemical parameters and renal tissue, which can be beneficial in the treatment of AKI. The treatment with the ethanolic extract is more potent than with the aqueous extract.

Evaluating the Proposed Mechanism by Which Atorvastatin Can Protect Renal Tissue against Contrast Media: An Animal study

2019 ◽  
pp. 75-84
Keyword(s):  
Contrast Media ◽  
Kidney Injury ◽  
Saline Group ◽  
Pleiotropic Effect ◽  
Renal Tissue ◽  
Male Rats ◽  
Dependent Manner ◽  
Group 2 ◽  
Dose Dependent ◽  
Media Group

Contrast- induced nephropathy (CIN) is an elevation of serum creatinine of ≥ 0.5 mg/dL from baseline after two to three days of exposure to contrast substance if there is no other cause for acute kidney injury. Atorvastatin may protect normal kidney physiology from contrast- induced kidney injury by effects unrelated to hypolipidemia termed pleiotropic effect by decline of endothelin production, angiotensin system down regulation, and under expression of endothelial adhesion molecules. This study was conducted to assess the strategy by which atorvastatin can achieve protective effect for kidneys after exposure to contrast media in an animal model. A 40 male rats were distributed randomly into 4 groups; ten rats for each: group (1): given normal saline; group (2): CIN group given iopromide as contrast media; group (3): given atorvastatin (20mg/kg) and iopromide; and group (4): given atorvastatin (40mg/kg) and iopromide. Blood collected by cardiac puncture for detection of serum glutathione, malondialdehyde, matrix metalloproteinase-9, and interleukin-18. The results have shown a significant increase in inflammatory and oxidative stress markers in contrast media group, and significant reduction in these markers in atorvastatin treated groups, in a dose-dependent manner. As conclusion, atorvastatin mechanism for protection against CIN in a dose-dependent manner can mediate by anti-inflammatory and antioxidant effects.

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