scholarly journals The effects of mometasone furoate and strontium chloride in a rat model of allergic rhinitis

2018 ◽  
Vol 97 (1-2) ◽  
pp. E1-E7
Author(s):  
Emine Elif Altuntaş ◽  
Ömer Tamer Doğan ◽  
Bülent Saraç ◽  
Nergiz Hacer Turgut ◽  
Kasım Durmuş ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Rat Model ◽  
Study Groups ◽  
Nerve Fibers ◽  
Mometasone Furoate ◽  
Albino Rats ◽  
Strontium Chloride ◽  
Histamine Dihydrochloride ◽  
Pharmacologic Treatments ◽  
Wistar Albino Rats

Neurogenic inflammation plays a role in the pathophysiology of allergic rhinitis. Highly effective in reducing the sensory irritation caused by some substances, strontium salts directly affect C-type nerve fibers. The aim of this study was to compare the efficacy of mometasone furoate and strontium chloride on early-phase symptoms in a rat model of allergic rhinitis. Wistar albino rats (n = 24) were randomly divided into three groups: the mometasone group, receiving 1 μg mometasone furoate (2 µl/site); the strontium 3% group, receiving 3% strontium chloride (2 μl/site); and the strontium 5% group, receiving 5% strontium chloride (2 μl/site). To induce significant nasal symptoms of allergic rhinitis, 5 µmol of histamine dihydrochloride (HDC) (2 µl/site) was administered. Symptoms of allergic rhinitis were recorded as frequencies of sneezing and nasal rubbing during a 15-minute interval. On days 1 and 2, respectively, 0.9% sodium chloride (NaCl) (2 μl/site to each nasal cavity) and HDC were administered in all of the study groups. On days 3 and 4, the study drugs were administered 10 and 30 minutes before the administration of HDC. On day 5, the study drugs were administered 10 minutes after the administration of HDC. The results of the present study revealed that when strontium chloride or mometasone furoate was administered 30 minutes before the onset of symptoms, a significant decrease was observed in sneezing and nasal rubbing. The number of sneezing occurrences was significantly lower and the number of nasal rubbing occurrences was higher in the strontium 3% group compared to the groups in which mometasone furoate and 5% strontium chloride were administered after onset of symptoms. Recent studies have investigated the efficacy and safety of strontium chloride nasal drops compared with common pharmacologic treatments of allergic rhinitis. These studies have revealed that allergic rhinitis can be successfully and safely treated with strontium-chloride-containing products, thus offering a potential new treatment strategy.

scholarly journals Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model

2019 ◽  
Vol 20 (4) ◽  
pp. 873 ◽  
Author(s):  
Nesrine Ebrahim ◽  
Yasser Mandour ◽  
Ayman Farid ◽  
Ebtesam Nafie ◽  
Amira Mohamed ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Adipose Tissue ◽  
Rat Model ◽  
Transforming Growth Factor ◽  
Immunoglobulin E ◽  
Physiological Saline ◽  
Transforming Growth Factor Β ◽  
Underlying Mechanism ◽  
Albino Rats ◽  
Microscopical Examination

This study was designed to investigate the potential effects and underlying mechanism of adipose tissue-derived mesenchymal stem cells (MSCs) on allergic inflammation compared to Montelukast as an antileukotriene drug in a rat model of allergic rhinitis (AR). The effect of MSCs was evaluated in albino rats that were randomly divided into four (control, AR, AR + Montelukast, and AR + MSCs) groups. Rats of AR group were sensitized by ovalbumin (OVA) and then challenged with daily nasal drops of OVA diluted in sterile physiological saline (50 μL/nostril, 100 mg/mL, 10% OVA) from day 15 to day 21 of treatment with/without Montelukast (1 h before each challenge) or MSCs I/P injection (1 × 106 MCSs; weekly for three constitutive weeks). Both Montelukast and MSCs treatment started from day 15 of the experiment. At the end of the 5th week, blood samples were collected from all rats for immunological assays, histological, and molecular biology examinations. Both oral Montelukast and intraperitoneal injection of MSCs significantly reduced allergic symptoms and OVA-specific immunoglobulin E (IgE), IgG1, IgG2a and histamine as well as increasing prostaglandin E2 (PGE2). Further analysis revealed that induction of nasal innate cytokines, such as interleukin (IL)-4 and TNF-α; and chemokines, such as CCL11 and vascular cell adhesion molecule-1 (VCAM-1), were suppressed; and transforming growth factor-β (TGF-β) was up-regulated in Montelukast and MSCs-treated groups with superior effect to MSCs, which explained their underlying mechanism. In addition, the adipose tissue-derived MSCs-treated group had more restoring effects on nasal mucosa structure demonstrated by electron microscopical examination.

The Effect of Minocycline on Fat Graft Survival and Apoptotic Pathway

2019 ◽  
Vol 35 (01) ◽  
pp. 096-102 ◽  
Author(s):  
Kırdar Güney ◽  
Bora Özel ◽  
Cemile Seymen ◽  
Çiğdem Elmas ◽  
Serhan Tuncer ◽  
...  
Keyword(s):  
Graft Survival ◽  
Study Groups ◽  
Fat Grafting ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Fat Graft ◽  
Albino Rats ◽  
Apoptotic Pathway ◽  
Fat Grafts ◽  
Dorsal Area ◽  
Wistar Albino Rats

AbstractVariable absorption rate is one of the biggest problems of fat grafting and one of the most important causes of fat graft volume loss is apoptosis. Minocycline is a tetracycline derivative and besides its antibacterial capacity, it has been widely using for anti-apoptotic effects. This study was designed to investigate the effect of minocycline on fat graft survival and adipocyte apoptosis. A total of two main and eight subgroups were designed and a total of 48 experimental animals, 6 in each group, were used. Fat grafts are obtained from Wistar albino rats and implanted to dorsal area of rats. Local and systemic minocycline was applied in the study groups. On the 9th day, apoptotic cells were detected by the terminal deoxynucleotidyl transferase dUTP nick end labeling method and on the 90th day morphologic characteristics and viability of adipocytes were evaluated using histologic and immunohistochemical methods and statistically compared. This study revealed that the fat grafts were bigger, and they kept their structures better and they were more vascular in the minocycline groups and apoptosis was significantly lower in the minocycline groups. The authors demonstrated that minocycline increases fat graft survival and statistical improvement in apoptosis inhibition via using minocycline therapy has been shown.

Can curcumin modulate allergic rhinitis in rats?

2016 ◽  
Vol 130 (12) ◽  
pp. 1103-1109 ◽  
Author(s):  
M Acar ◽  
N Bayar Muluk ◽  
S Yigitaslan ◽  
B P Cengiz ◽  
P Shojaolsadati ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Goblet Cell ◽  
Histopathological Examination ◽  
Nasal Congestion ◽  
Control Group ◽  
Albino Rats ◽  
Epithelial Surface ◽  
Vascular Proliferation ◽  
Wistar Albino Rats ◽  
Goblet Cell Metaplasia

AbstractObjectives:This study aimed to explore the effects of curcumin on experimental allergic rhinitis in rats.Methods:Twenty-eight male Wistar albino rats were randomly divided into four groups: a control group; a group in which allergic rhinitis was induced and no treatment given; a group in which allergic rhinitis was induced followed by treatment with azelastine hydrochloride on days 21–28; and a group in which allergic rhinitis was induced followed by treatment with curcumin on days 21–28. Allergy symptoms and histopathological features of the nasal mucosa were examined.Results:The sneezing and nasal congestion scores were higher in the azelastine and curcumin treatment groups than in the control group. Histopathological examination showed focal goblet cell metaplasia on the epithelial surface in the azelastine group. In the curcumin group, there was a decrease in goblet cell metaplasia in the epithelium, decreased inflammatory cell infiltration and vascular proliferation in the lamina propria.Conclusion:Curcumin is an effective treatment for experimentally induced allergic rhinitis in rats.

Cadmium acts as a silent killer of liver by inducing oxidative stress and hepatocellular injury and a possible amelioration by vitamin B12 and folic acid in rat model

2019 ◽  
Vol 1 ◽  
pp. 105-117
Author(s):  
A. Banerjee ◽  
P. Nandi ◽  
C. Bhattacharya ◽  
Z. Kabir ◽  
S. Mukherjee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Folic Acid ◽  
Vitamin B12 ◽  
Rat Model ◽  
Male Rats ◽  
Albino Rats ◽  
Therapeutic Effects ◽  
Hepatocellular Injury ◽  
Male Adult ◽  
Wistar Albino Rats

<br/><b>Purpose:</b> To investigate the involvement of oxidative stress in Cadmium (Cd) induced alteration in the functional status of the liver. And to assess the efficacy of folic acid and vitamin B12 in preventing Cd-induced damage in the same. <br/><b>Materials and methods:</b> The experiment was carried out for four weeks. For the experiment, 25 healthy male adult Wistar albino rats were randomly selected and were divided into five equal groups and treated as control, treated with Cd, supplemented with vitamin B12 and folic acid and in the combination of these two. After 28 days the liver function enzymes and oxidative stress parameters were measured. <br/><b>Results:</b> Cd is the silent killer of the hepatic system through the induction of oxidative stress in male rats. From this investigation, it is evident that the folic acid+vitamin B12 possess significant hepatoprotective and antioxidant activity against Cd-induced hepatotoxicity in the rat model. In addition, results revealed that the folic acid alone and or in combination with vitamin B12 blunted the hepatotoxic effect significantly. <br/><b>Conclusions:</b> Based on results obtained, it can be concluded that folic acid and vitamin B12 offer a protective effect in Cd-induced oxidative stress associated with hepatocellular injury. Folic acid and vitamin B12 can be considered as a potent natural antioxidant which has the capacity to provide protection against Cd-induced oxidative stress in the liver in rats. However, to elucidate the exact mechanism of this modulatory effect and to examine its potential therapeutic effects further studies are essential.

scholarly journals Strontium Chloride: Can It Be a New Treatment Option for Ulcerative Colitis?

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Firdevs Topal ◽  
Ozlem Yonem ◽  
Nevin Tuzcu ◽  
Mehmet Tuzcu ◽  
Hilmi Ataseven ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Experimental Colitis ◽  
Histological Evaluation ◽  
Albino Rats ◽  
Strontium Chloride ◽  
Significant Difference ◽  
Healthy Control ◽  
Group 2 ◽  
New Treatment ◽  
Wistar Albino Rats

Background/Aims. Patients with ulcerative colitis still need effective therapy without major side effects. It has been found that strontium can suppress NFκB activation induced by TNF-α. This opens a gate to a new anti-TNF agent which is cheap and can be given orally. We for the first time aimed to investigate the effect of strontium chloride (SrCl2) on inflammation in experimental colitis.Methods. Thirty female Wistar albino rats were divided into 5 groups each containing 6 rats. The rats in groups 1 and 2 served as the healthy control and colitis group, respectively. The rats in groups 3, 4, and 5 had colitis and received 40 mg/kg SrCl2, 160 mg/kg SrCl2, and 1 mg/kg prednisolone by oral gavage, respectively. The rats were sacrificed for histological evaluation and determination of serum neopterin, TNF-α, and IFN-γlevels.Results. The neopterin, TNF-αand IFNγlevels of group 2 was significantly higher than the other groups. The neopterin, TNF-α, and IFN-γlevels of controls and other treatment groups were comparable. There were a significant difference in macroscopic and microscopic healing between group 2 and other groups histologically. But there was not a significant difference within treatment receiving groups.Conclusion. SrCl2had comparable therapeutic efficiency with prednisolone.

scholarly journals Contribution of Autologous Omentum Transposition to the Regeneration of Renal Injuries in the Rat Model

2020 ◽  
Author(s):  
Tayfun Bilgic ◽  
Umit Ince ◽  
Fehmi Narter
Keyword(s):  
Rat Model ◽  
Primary Repair ◽  
Healing Process ◽  
Histological Finding ◽  
Kidney Injury ◽  
Study Groups ◽  
Renal Tissue ◽  
Albino Rats ◽  
Loss Of Function ◽  
Renal Injuries

Abstract Aim: After renal trauma, surgical treatment is vital, but sometimes there may be loss of function due to fibrosis. We aimed to evaluate the repair effect of transpositioned autologous omentum on injured renal tissue in a rat model. Methods: A total of 30 female Wistar Albino rats were included and they were randomly separated into a sham group and four study groups. Iatrogenic renal injuries were repaired using a surgical technique (primary repair 1 and 2 groups) or transpositioned autologous omentum (omentum repair 1 and 2 groups). In all groups, blood samples were taken preoperatively and on the 7 th postoperative day in all groups and also on the 18 th postoperative day in the control and two study groups. All rats were sacrificed on the 7 th or 18 th day postoperatively and their right kidneys were taken for histopathological evaluation.Results: There was a trend toward decrease in urea and creatinine levels in all the groups. There was no significant correlation between urea and creatinine levels and histological finding scores. The omentum repair group had significantly lower inflammation and granulation scores compared with the primary repair and sham groups. There was a significant and positive correlation between inflammation and granulation and fibrosis scores. There was a significant and negative correlation between healing completion score and either inflammation and granulation scores. There were also positive correlations between histological findings in the kidney specimen and surrounding tissues. Conclusion: The use of the autologous omentum tissue for repair of kidney injury had attenuation effects on inflammation and granulation compared with primary repair. These results imply that use of omentum tissue to facilitate healing of kidney injury may theoretically lead to a more effective healing process and reduced fibrosis and tissue and function loss. These potentially beneficial effects of autologous omentum tissue should be investigated in further well-designed experimental and clinical studies.

scholarly journals Effectiveness of oral administration of peppermint and coriander extracts on cognition in Scopolamine induced rat model of amnesia

2020 ◽  
Vol 11 (3) ◽  
pp. 4222-4226
Author(s):  
Jasira Sirajudheen ◽  
Sai Sailesh Kumar Goothy ◽  
Mukkadan J K
Keyword(s):  
Oral Administration ◽  
Rat Model ◽  
Positive Impact ◽  
Neurological Diseases ◽  
Control Group ◽  
Albino Rats ◽  
Study Results ◽  
Peppermint Extract ◽  
Neuroprotective Actions ◽  
Wistar Albino Rats

Herbs and spices have been shown to be very effective in the management of the neurological disorders. They have neuroprotective actions that prevent the deterioration of the memory and other cognitive functions in the neurological diseases. Peppermint (menthe×piperita, also known as M. balsamea wild) is a hybrid mint, a cross between water mint and spearmint. Peppermint consists of several medicinal components and research was testified safety of consumption of the peppermint. The study compared the memory-boosting and regaining effects of oral administration of peppermint and coriander extracts. 36 male and female Wistar albino rats were assigned into three groups randomly that is control, coriander and peppermint groups, respectively. A control group (n=6) received neither peppermint nor coriander administered (milk was administered). Peppermint group (n=6) received peppermint extract. Coriander group (n=6) received coriander extract. Amnesia rat model was prepared by the administration of as intraperitoneal injection of scopolamine. The R-maze and T-maze tasks was conducted as mentioned in the literature. Mean trials of acquisition is significantly less (P<0.01) in the peppermint group. The number of mean trials of retention is significantly less (P<0.01) in the peppermint group. The mean trials for retention of the coriander group is significantly (P<0.05) less than the peppermint group. The study results support a positive impact of coriander and peppermint on cognition. The study recommends further detailed studies to support the administration of these extracts in the management of neurological diseases that deteriorates cognition.

Amelioration of radiation-induced lung injury by halofuginone: An experimental study in Wistar–Albino rats

2016 ◽  
Vol 36 (6) ◽  
pp. 638-647 ◽  
Author(s):  
M Calik ◽  
G Yavas ◽  
SG Calik ◽  
C Yavas ◽  
ZE Celik ◽  
...  
Keyword(s):  
Lung Injury ◽  
Chronic Inflammation ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Light And Electron Microscopy ◽  
Study Groups ◽  
Albino Rats ◽  
Long Term Follow Up ◽  
Radiation Induced ◽  
Wistar Albino Rats

To evaluate effects of halofuginone (H) on radiation-induced lung injury (RILI), 60 rats were divided into six groups: Group (G) 1 control, G2 radiotherapy (RT) only, G3 and G4 2. 5 and 5 μg H and G5 and G6 RT + 2.5 and 5 μg H groups, respectively. A single dose of 12 Gy RT was given to both lungs. H was applied intraperitoneally with daily doses, until animals were killed at 6 and 16 weeks after RT. At 6th and 16th weeks of RT, five rats from each group were killed. Lung tissues were dissected for light and electron microscopy. Chronic inflammation, fibrosis and transforming growth factor-beta (TGF)-β scores of all study groups were significantly different at 6th and 16th week ( p < 0.001). Chronic inflammation, fibrosis and TGF-β scores of G2 were higher than G5 and G6 at 6th and 16th weeks of RT. At 16th week, fibrosis and TGF-β scores of G5 were higher than G6 ( p = 0.040 and 0.028, respectively). Electron microscopical findings also supported these results. Therefore, H may ameliorate RILI. The effect of the H was more prominent at higher dose and after long-term follow-up. These findings should be clarified with further studies.

Efficacy of Tenoxicam on Intra-Abdominal Adhesion Prevention in a Rat Model

1996 ◽  
Vol 24 (4) ◽  
pp. 352-357 ◽  
Author(s):  
T Yilmazlar ◽  
E Kaya ◽  
E Gürpinar ◽  
H Emiroğlu
Keyword(s):  
Rat Model ◽  
Abdominal Cavity ◽  
Adhesion Formation ◽  
Test Material ◽  
Adhesion Prevention ◽  
Control Group ◽  
Albino Rats ◽  
Abdominal Adhesion ◽  
Wistar Albino Rats ◽  
The Right

The aim of this study was to investigate the effect of tenoxicam as a non-steroidal anti-inflammatory drug (NSAID) on intra-abdominal adhesion prevention in a rat model. Altogether 50 Wistar-Albino rats weighing 220 – 280 g were assigned to five groups, each of which was made up of 10 rats. All the rats were anaesthetized and prepared for sterile surgery. After a mid-line laparotomy was performed, a 1 cm area of the caecum was rubbed with gauze until subserosal haemorrhage developed, and then a 5 mm-diameter part of the peritoneum on the right side of the abdominal wall was removed. Prior to complete closure, 3 ml of the test material was placed into the abdominal cavity. On the eighth day the rats were killed and the adhesion score was determined. The groups and their mean adhesion scores were as follows: control group (normal saline), 2.5; group of dilution buffer, 1.8; tenoxicam (0.125 mg/kg), 1.3; tenoxicam (0.25 mg/kg), 1.3; and tenoxicam (0.5 mg/kg), 0.9. The differences between the adhesion scores among all the groups ( P < 0.05, Kruskal-Wallis test), and those between the tenoxicam groups and control group ( P < 0.05, Mann-Whitney U-test), were significant. Thus a single instillation of tenoxicam into the peritoneal cavity at the time of surgery reduced adhesion formation effectively in this model, irrespective of dosage.

scholarly journals Assessment of Laser Biostimulation in Induction of Ovulation

2018 ◽  
Vol 6 (7) ◽  
pp. 1193-1198
Author(s):  
Mohamed A. El-Nourya ◽  
Mahmoud B. El-Begawyb ◽  
Mona E. Morsyc ◽  
Ehab M. Al Sherbiny
Keyword(s):  
Clomiphene Citrate ◽  
Study Groups ◽  
Control Group ◽  
Albino Rats ◽  
Serum Estradiol ◽  
Serum Progesterone ◽  
Induction Of Ovulation ◽  
Significant Difference ◽  
Conventional Medical Therapy ◽  
Wistar Albino Rats

AIM: This study aimed to evaluate a new modality of low power laser biostimulation in rat ovaries, in comparison with the conventional medical therapy by clomiphene citrate that depends on up-regulation of the hypothalamic-pituitary-ovarian axis to induce ovulation.STUDY DESIGN: A Prospective experimental study carried out from January 2014 to February 2016.SETTING: University-based photobiology laboratory.MATERIALS AND METHODS: Seventy-two (72) Female-Wistar Albino rats were used in this study, divided into three groups: 17 rats used as a control group, 19 rats received clomiphene citrate (Clomid group), 36 rats exposed to diode laser 660 nm wavelength (laser group).RESULTS: Biochemical assessment of serum Estradiol and serum Progesterone was done in the three study groups. Serum Estradiol & Progesterone levels were statistically significantly higher in clomiphene and laser treated groups than non-interventional controls, while no significant difference between clomiphene and laser groups as regard of both hormones.CONCLUSION: This study shows that ovarian laser biostimulation is a new encouraging method for induction of ovulation, at least in animals. This had been proven biochemically by a significant increase in serum Estradiol and serum Progesterone.

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