scholarly journals Stimulation of Phenolic Compounds Accumulation and Antioxidant Activity in In Vitro Culture of Salvia Tebesana Bunge in Response to Nano-TiO2 and Methyl Jasmonate Elicitors

2021 ◽  
Author(s):  
Amin allah Shoja ◽  
Monireh - Cheniany ◽  
Ali Ganjeali
Keyword(s):  
Phenolic Compounds ◽  
Methyl Jasmonate ◽  
Rosmarinic Acid ◽  
Apical Meristem ◽  
Leaf Explants ◽  
Maximum Level ◽  
Good Technique ◽  
Phenolic Derivatives ◽  
Derivatives Of

Abstract In the present study, we first attempted to achieve an efficient procedure for optimizing callogenesis from apical meristem and leaf explants of S. tebesana on MS media containing different concentrations of BAP alone and in combination with 2,4-D. Then, the inducing effect of nano-TiO2 (10, 60, and 120 mg L− 1) and methyl jasmonate (50, 100, and 200 µM), as abiotic elicitors were studied on the enhancement of phenolic compounds, rosmarinic acid, and some individual flavonoids as well as antioxidant capacity of callus extracts. According to the results, the highest callogenesis rate (100 and 93.33, respectively) and DW (0.55 ± 0.03 and 0.36 ± 0.02 g, respectively) per responsive explant were achieved from apical meristem on MS media containing "BAP 1 + 2,4-D 1" mg L− 1 and from leaf explant on the medium supplemented with "BAP 0.5 + 2,4-D 1" mg L− 1. The elicitation with 10 and 60 mg L− 1 nano-TiO2 (respectively for apical meristem and leaf), and 50µM MeJa could significantly promote the production of predominant phenolic derivatives in S. tebesana calli, where the highest content of total phenolics, O-diphenols, phenolic acid, flavonoid, flavone and flavonol, proanthocyanidin was recorded. Additionally, in increasing the amount of rosmarinic acid of callus, nano-TiO2 treatment was more effective than the elicitation with MeJa. Also, the highest content of Apigenin (0.33 ± 0.02 µg g− 1 DW) was detected after MeJa-elicitation (50µM), while the maximum level of Quercetin (2.61 ± 0.09 µg g− 1 DW) and Rutin (13.79 ± 08 µg g− 1 DW) were obtained after exposure to 60 mg L− 1 nano-TiO2, both from leaf-derived calli. While a significant positive correlation was recorded between antioxidant assays (DPPH and FRAP) and phenolic derivatives of treated calli; a very strong correlation occurred between the content of rosmarinic acid of apical meristem-derived calli and DPPH and FRAP values (r2 = -0.921 and r2 = -0.913, P < 0.01 respectively). Our results showed that the combination of in vitro culture and elicitation would be a good technique to successfully produce and enhance the content of pharmacologically valuable metabolites in S. tebesana.

scholarly journals The effect of aseptic cultivation of Crambe mitridatis Juz. plants on its biochemical composition

2020 ◽  
Vol 17 (2) ◽  
pp. 172-178
Author(s):  
N. O. Pushkarova ◽  
T. M. Kyrpa-Nesmiian ◽  
M. V. Kuchuk
Keyword(s):  
Phenolic Compounds ◽  
Biochemical Composition ◽  
Leaf Explants ◽  
Biologically Active Compounds ◽  
Hydroxycinnamic Acids ◽  
Biologically Active ◽  
Active Compounds ◽  
Petiole Explants ◽  
Aseptic Conditions

The aim of the research was to establish efficient microclonal propagation conditions of endangered Crambe mitridatis plants in vitro and to study the possible effect of aseptic cultivation on biochemical composition (hydroxycinnamic acids, flavonoids, phenolic compounds) of plants. Methods. In vitro plant culture methods were applied. Seeds were used for aseptic culture initiation. Morphogenic potential of root, leaf and petiole explants was studied on Murashige-Skoog medium with addition of plant growth regulators. The content of biologically active compounds was measured using spectrometry in plants grown in aseptic conditions and in the greenhouse. Results. Morphogenic potential of root, leaf and petiole explants was studied and the highest regeneration frequency of plantlets was established for root explants (80 %), for petiole explants (50 %) and the lowest for leaf explants (20 %). It was found that plants cultivated in aseptic conditions have higher hydroxycinnamic acids, flavonoids and phenolic compounds compared to plants grown in vivo. Conclusions. It is advisable to multiply C. mitridatis plants in vitro via root and petiole explants. Aseptic cultivation contributes to synthesis of biologically active compounds (auxin synergists) in C. mitridatis plants.Keywords: in vitro culture, hydroxycinnamic acids, flavonoids, phenolic compounds, Crambe mіtridatis.

scholarly journals New Phenolic Derivatives of Thiazolidine-2,4-dione with Antioxidant and Antiradical Properties: Synthesis, Characterization, In Vitro Evaluation, and Quantum Studies

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2060 ◽  
Author(s):  
Gabriel Marc ◽  
Anca Stana ◽  
Smaranda Dafina Oniga ◽  
Adrian Pîrnău ◽  
Laurian Vlase ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Root Cause ◽  
Naturally Occurring ◽  
Antiradical Properties ◽  
Radical Generation ◽  
Phenolic Derivatives ◽  
Energetic Level ◽  
Derivatives Of ◽  
Phenol Moiety

Oxidative stress has been incriminated in the physiopathology of many diseases, such as diabetes, cancer, atherosclerosis, and cardiovascular and neurodegenerative diseases. There is a great interest in developing new antioxidants that could be useful for preventing and treating conditions for which oxidative stress is suggested as the root cause. The thiazolidine-2,4-dione derivatives have been reported to possess various pharmacological activities and the phenol moiety is known as a pharmacophore in many naturally occurring and synthetic antioxidants. Twelve new phenolic derivatives of thiazolidine-2,4-dione were synthesized and physicochemically characterized. The antioxidant capacity of the synthesized compounds was assessed through several in vitro antiradical, electron transfer, and Fe2+ chelation assays. The top polyphenolic compounds 5f and 5l acted as potent antiradical and electron donors, with activity comparable to the reference antioxidants used. The ferrous ion chelation capacity of the newly synthesized compounds was modest. Several quantum descriptors were calculated in order to evaluate their influence on the antioxidant and antiradical properties of the compounds and the chemoselectivity of the radical generation reactions has been evaluated. The correlation with the energetic level of the frontier orbitals partially explained the antioxidant activity, whereas a better correlation was found while evaluating the O–H bond dissociation energy of the phenolic groups.

scholarly journals PHYTOCHEMICAL ANALYSIS OF CALLUS TWO VARIETIES ORTHOSIPHON ARISTATUS (BLUME) MIQ ON MURASHIGE AND SKOOG MEDIA: A STRATEGIC STEP OF SECONDARY METABOLITE PRODUCTION

2021 ◽  
pp. 71-77
Author(s):  
FAHRAUK FARAMAYUDA ◽  
TOTIK SRI MARIANI ◽  
ELFAHMI ◽  
SUKRASNO
Keyword(s):  
Secondary Metabolites ◽  
Rosmarinic Acid ◽  
High Performance ◽  
Leaf Explants ◽  
Gradient Elution ◽  
Growth Media ◽  
Phytochemical Analysis ◽  
New Information ◽  
Sterile Leaf

Objective: The research aimed to provide new information regarding the secondary metabolites content of purple and white-purple Orthosiphon aristatus (Blume) Miq. callus, which can then be used as a basis for developing towards cell suspension and ultimately producing secondary metabolites using bioreactors. Methods: Callus induction of two varieties of O. aristatus were performed by inoculating sterile leaf explants grown on Murashige and Skoog basal media supplemented with 2,4-dichlorophenoxyacetis acid 0.4 ppm. The secondary metabolites were analysed and quantified using high-performance liquid chromatography with gradient elution. Results: The results showed the growth of callus two varieties of O. aristatus in growth media MS with 2,4-D 0.4 ppm. Rosmarinic acid content in the acetone extract of the purple variety callus was 1.28% w/w, and the white-purple variety was 2.22% w/w. Conclusion: This study could form the basis for the development of rosmarinic acid production by In vitro culture modification.

scholarly journals Elicitor induced increased rosmarinic acid content of in vitro root cultures of Ocimum basilicum L. (Sweet Basil)

2020 ◽  
Vol 7 (2) ◽  
pp. 157-163
Author(s):  
Trayee Biswas
Keyword(s):  
Methyl Jasmonate ◽  
Rosmarinic Acid ◽  
High Performance ◽  
Biological Activities ◽  
Fold Increase ◽  
Ocimum Basilicum ◽  
Total Phenol Content ◽  
Root Cultures ◽  
Shoot Bud

In vitro root cultures provide an alternative means for producing secondary metabolites of pharmaceutical and industrial importance. The present investigation is an attempt to study the effect of methyl jasmonate (MeJA) as an elicitor, on the production of rosmarinic acid through in vitro root cultures of Ocimum basilicum L. The rosmarinic acid is a preformed constitutively accumulated defense compound having several biological activities including antibacterial, antiviral, and antioxidative activity. Uniform progeny of O. basilicum L. was obtained through in vitro shoot bud multiplication as reliable source of explants. In vitro root cultures were established in one fourth strength of Murashige and Skoog (MS) liquid medium supplemented with 1.0 mg l–1 ?-napthaleneacetic acid (NAA). Methyl jasmonate, was supplemented to the root cultures at different concentrations (0.01, 0.1 and 1.0 mM), and their effect on total phenol content as well as rosmarinic acid production was studied. Rosmarinic acid was quantified by high performance liquid chromatography (HPLC). Compared to the control set of cultures 1.60 fold increase in rosmarinic acid concentration (4.05 ± 0.74 % of dry wt) was observed in elicitor treated cultures.

scholarly journals Isodon rugosus (Wall. ex Benth.) Codd In Vitro Cultures: Establishment, Phytochemical Characterization and In Vitro Antioxidant and Anti-Aging Activities

2019 ◽  
Vol 20 (2) ◽  
pp. 452 ◽  
Author(s):  
Bilal Abbasi ◽  
Aisha Siddiquah ◽  
Duangjai Tungmunnithum ◽  
Shankhamala Bose ◽  
Muhammad Younas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Rosmarinic Acid ◽  
Phenolic Content ◽  
Leaf Explants ◽  
Total Phenolic ◽  
Pentacyclic Triterpenoids ◽  
Isodon Rugosus ◽  
Glycation End Products ◽  
Phytochemical Profiles

Isodon rugosus (Wall. ex Benth.) Codd accumulates large amounts of phenolics and pentacyclic triterpenes. The present study deals with the in vitro callus induction from stem and leaf explants of I. rugosus under various plant growth regulators (PGRs) for the production of antioxidant and anti-ageing compounds. Among all the tested PGRs, thidiazuron (TDZ) used alone or in conjunction with α-napthalene acetic acid (NAA) induced highest callogenesis in stem-derived explants, as compared to leaf-derived explants. Stem-derived callus culture displayed maximum total phenolic content and antioxidant activity under optimum hormonal combination (3.0 mg/L TDZ + 1.0 mg/L NAA). HPLC analysis revealed the presence of plectranthoic acid (373.92 µg/g DW), oleanolic acid (287.58 µg/g DW), betulinic acid (90.51 µg/g DW), caffeic acid (91.71 µg/g DW), and rosmarinic acid (1732.61 µg/g DW). Complete antioxidant and anti-aging potential of extracts with very contrasting phytochemical profiles were investigated. Correlation analyses revealed rosmarinic acid as the main contributor for antioxidant activity and anti-aging hyaluronidase, advance glycation end-products inhibitions and SIRT1 activation, whereas, pentacyclic triterpenoids were correlated with elastase, collagenase, and tyrosinase inhibitions. Altogether, these results clearly evidenced the great valorization potential of I. rugosus calli for the production of antioxidant and anti-aging bioactive extracts for cosmetic applications.

scholarly journals Expression Analysis of Phenylpropanoid Pathway Genes and Metabolomic Analysis of Phenylpropanoid Compounds in Adventitious, Hairy, and Seedling Roots of Tartary Buckwheat

Plants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 90
Author(s):  
Minsol Choi ◽  
Ramaraj Sathasivam ◽  
Bao Van Nguyen ◽  
Nam Il Park ◽  
Sun-Hee Woo ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Leaf Explants ◽  
Tartary Buckwheat ◽  
Pcr Analysis ◽  
Naphthaleneacetic Acid ◽  
Root Induction ◽  
Root Cultures ◽  
Fagopyrum Tataricum ◽  
Seedling Roots

Tartary buckwheat (Fagopyrum tataricum) is an important crop that belongs to the Polygonaceae family, whose roots have received considerable attention due to the presence of compounds with high nutritional and medicinal value. In this study, we aimed to develop an efficient protocol for the culture of adventitious (ARs) and hairy (HRs) roots on a half-strength Schenk and Hildebrandt (SH) medium containing different concentrations of the auxins, α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), and indole-3-acetic acid (IAA). The highest percentage of root induction (91.67%) was achieved with 0.5 mg/L IAA, whereas the greatest number of roots was found in 1 mg/L IAA. In contrast, 0.1 mg/L IBA returned the longest roots. As expected, HRs were obtained from in vitro leaf explants infected with Agrobacterium rhizogenes R1000. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of 11 phenolic pathway genes revealed that five genes (FtPAL, FtC3H, FtHQT, FtCHS, and FtANS) were highly expressed in HRs, whereas only four (FtC4H, FtFLS2, FtDFR, and FtANR), and three (Ft4CL, FtCHI, and FtF3H) were recognized in the ARs and seedling roots (SRs), respectively. HPLC analysis of phenolic compounds in different root cultures showed that the majority of the phenolic compounds (both individual and total) were significantly accumulated in the HRs. Principal component analysis (PCA) identified differences among the three root types, whereby HRs were separated from ARs and SRs based on the amount of phenolic compounds present. Analysis of the metabolic pathway revealed that among the identified metabolites, the 3, 2, and 1 pathways were associated with flavonoid, flavone and flavonol, and phenylpropanoid biosynthesis, respectively. Hierarchical clustering analysis and the heat map showed that the different root cultures presented unique metabolites.

scholarly journals THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE

2021 ◽  
Vol 14 (2) ◽  
pp. 59-66
Author(s):  
M. O. Twardovska ◽  
Keyword(s):  
Tissue Culture ◽  
Phenolic Compounds ◽  
Hplc Analysis ◽  
Leaf Explants ◽  
Biologically Active ◽  
Deschampsia Antarctica ◽  
Tissue Cultures ◽  
Polar Metabolites ◽  
Selection Of

Aim. The aim of the study was to determine the quantitative and qualitative content of phenolic compounds and flavonoids in Deschampsia antarctica E. Desv. tissue cultures obtained from plants originating from different islands of the maritime Antarctic. Methods. In vitro tissue culture, Folin-Ciocalteu method, spectrophotometry, HPLC analysis. Results. The quantitative content of phenolic compounds and flavonoids in D. antarctica tissue cultures obtained from plants of six genotypes (DAR12, DAR13, G/D12-2a, Y66, R30 and L57) was determined. The highest content of phenolic compounds (4.46 and 3.75 mg/g) was found in tissue cultures obtained from root and leaf explants of plant genotype L57. The highest amount of flavonoids (7.17 mg/g) was accumulated in G/D12-2a tissue culture of root origin. The content of the studied biologically active compounds (BACs) did not change with increasing number of subculture generations (from passage 10 to 19). HPLC analysis showed that in D. antarctica tissue cultures, a shift in the biosynthesis of BACs occurred towards the synthesis of more polar metabolites compared to explant donor plants. Conclusions. It was found that the transition of cells to undifferentiated growth affected the content of BACs, the amount of which decreased 2–5 times simultaneously with a significant change in their profile. This provided a basis for further biochemical studies, as well as for careful selection of tissue culture of D. antarctica to use it as a potential source of BACs.

scholarly journals Effect of Elicitation with (+)-Usnic Acid on Accumulation of Phenolic Acids and Flavonoids in Agitated Microshoots of Eryngium alpinum L.

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5532
Author(s):  
Małgorzata Kikowska ◽  
Barbara Thiem ◽  
Karolina Jafernik ◽  
Marta Klimek-Szczykutowicz ◽  
Elżbieta Studzińska-Sroka ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
Usnic Acid ◽  
Shoot Cultures ◽  
Biomass Growth ◽  
Effective Technique ◽  
In Vitro Shoot Cultures

The present work was aimed at studying the potential of elicitation on the accumulation of phenolic compounds in in vitro shoot cultures of Eryngium alpinum L., a protected plant from the Apiaceae family. The study examined the influence of (+)-usnic acid on the biomass growth as well as on the biosynthesis of the desired flavonoids and phenolic acids in the cultured microshoots. The phenolic compound content was determined by HPLC-DAD. The flavonoid of the highest concentration was isoquercetin, and the phenolic acids of the highest amount were rosmarinic acid, caffeic acid and 3,4-dihydroxyphenylacetic acid, both in the non-elicited and elicited biomass. Isoquercetin accumulation was efficiently increased by a longer elicitation with a lower concentration of lichenic compound (107.17 ± 4.67 mg/100 g DW) or a shorter elicitation with a higher concentration of acid (127.54 ± 11.34 and 108.37 ± 12.1 mg/100 g DW). Rosmarinic acid production generally remained high in all elicited and non-elicited microshoots. The highest content of this acid was recorded at 24 h of elicitation with 3.125 µM usnic acid (512.69 ± 4.89 mg/100 g DW). The process of elicitation with (+)-usnic acid, a well-known lichenic compound with allelopathic nature, may therefore be an effective technique of enhancing phenolic compound accumulation in alpine eryngo microshoot biomass.

In vitro flowering on leaf explants of Streptocarpus nobilis. The influence of culture medium components on vegetative and reproductive development

1982 ◽  
Vol 60 (8) ◽  
pp. 1461-1468 ◽  
Author(s):  
John Simmonds
Keyword(s):  
Sucrose Concentration ◽  
Leaf Explants ◽  
Maximum Level ◽  
Reproductive Development ◽  
Proximal Region ◽  
In Vitro Flowering ◽  
Flower Buds ◽  
Vegetative Development ◽  
Sucrose Medium

Streptocarpus nobilis (C.B. Clarke) became photoperiodically more responsive to short days with increasing age. Leaf explants from photoperiodically induced plants were all organogenically competent when cultured on Murashige and Skoog medium in 8-h photoperiods. Both vegetative and flower buds were developed; the maximum level of in vitro flowering occurred in response to 6-benzylaminopurine (BAP), but only 50% of the explants produced flowers. 3-Indoleacetic acid (IAA) (1 × 10−7 to 1 × 10−6 M) inhibited in vitro flowering. A nutrient medium was devised which allowed the expression of flowering on 100% of explants. The expression of vegetative or reproductive development was strongly influenced by the concentrations of media nutrients. On high-KNO3 (20 mM) – high-sucrose (100 mM) medium vegetative development was favoured and flower buds were produced on only 28% of explants. On low-KNO3 (2 mM) – low-sucrose (15 mM) medium only flower buds were developed on all explants. On low-KNO3 (5 mM) medium organogenesis was also affected quantitatively by the sucrose concentration; organogenesis was reduced from 90% explant response on 25-mM sucrose medium to 52% explant response on 100-mM sucrose medium. The most rapid flowering response to low-KNO3 (2 mM) – low-sucrose (15 mM) medium was obtained on explants from leaves of axillary branches. Flower buds were formed on the main vein at the proximal region of the explant. This high response at a predictable location provides a useful system for investigating flowering.

scholarly journals Phenolic Profile and Antioxidant Activity of Ethanolic Extract of Larrea cuneifolia Cav. Leaves

Proceedings ◽  
2020 ◽  
Vol 70 (1) ◽  
pp. 37
Author(s):  
Maria Emilia Lorenzo ◽  
Patricia Elizabeth Gómez ◽  
Eugenia Sabatino ◽  
Adrián Federico Segovia ◽  
Lara Carolina Figueroa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Central Valley ◽  
Cell Types ◽  
Phenolic Profile ◽  
Antioxidant Characteristics ◽  
Derivatives Of

The genus of the Zygophyllaceae family includes evergreen shrub species. Background highlights the antioxidant and anti-tumor activity of Larrea divaricate and nordihydroguayaretic acid (NDGA) due to their potential as a dietary supplement and food preservative, but little is known about Larrea cuneifolia. The aim of this work was to determine the antioxidant characteristics of ethanolic extracts of L. cuneifolia leaves collected in the central valley of Catamarca (Argentina). Total polyphenols content (TP) was determined by Folin-Ciocâlteu and the phenolic profile by HPLC-PDA-QTOF. The antioxidant activity was measured by in vitro (FRAP, TEAC and DPPH) and cellular (HepG2 and Caco2 cells) assays. The phenolic compounds identified were mainly derivatives of NDGA and flavonols derivatives of quercetin, kaempferol, isorhamnetin and gossypetin. TP content and antioxidant activity exceeded the values reported for L. divaricata. With regard to cytotoxicity, an increase in this parameter could be observed with the increase in the concentration of polyphenols in both cell types. Furthermore, in cells exposed to H2O2, a significant decrease in reactive oxygen species (ROS) concentration was observed for HepG2 cells. This effect can be used to study compounds with bioactivity on tumor cells. L. cuneifolia is a species rich in phenolic compounds, with antioxidant properties, and is a potential source of bioactive compounds for the production of functional foods.

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