Antioxidants Supplementation In Acute Amitriptyline Abuse for Pain

2021 ◽  
Author(s):  
Hameed Kadar Ali S ◽  
Wasim Ali Raja K ◽  
IRFAN Navabshan ◽  
Mohammad Habeeb ◽  
Ismail Y
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Lipoic Acid ◽  
Standard Treatment ◽  
Human Subjects ◽  
Control Group ◽  
P Value ◽  
Alpha Lipoic Acid ◽  
Group V ◽  
Group I

Abstract The fundamental aim of this study is to establish the role of anti-oxidant supplementation in alleviating acute amitriptyline induced oxidative stress. The effect of supplementation was compared on treatment of acute amitriptyline intoxication cases for pain management, with Alpha lipoic acid (ALA) alone or with vitamin C, with that of healthy individuals (Group I), and those receiving only routine standard treatment (RST) as control (Group II). Total of 132 human subjects divided into 5 groups were supplemented with either placebo, RST, RST with Vitamin C, RST with ALA, or RST with Vitamin C and ALA. Results of this study revealed that the decrease in the level of oxidative stress and enzyme activity was observed among those supplemented with either Alpha lipoic acid alone or along with vitamin C, with a slightly more decrease in the latter group. P value < 0.001 was considered statistically significant. The percentage of benefit of treatment on supplementation with vitamin C and Alpha lipoic acid showed a marked increase in-group V cases after supplementation with both in combination. The results provided that the oxidative stress induced by acute amitriptyline poisoning is comparatively decreased by supplementation with antioxidants like Alpha lipoic acid and Vitamin C, than those only on routine standard treatment.

scholarly journals Therapeutic Effects of the Combination of Alpha-Lipoic Acid (ALA) and Coenzyme Q10 (CoQ10) on Cisplatin-Induced Nephrotoxicity

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Eman Aly Khalifa ◽  
Ahmed Nabil Ahmed ◽  
Khalid Shaaban Hashem ◽  
Ahmad Gad Allah
Keyword(s):  
Combination Therapy ◽  
Lipoic Acid ◽  
Coenzyme Q10 ◽  
Renal Damage ◽  
Control Group ◽  
Prophylactic Effect ◽  
Alpha Lipoic Acid ◽  
Group V ◽  
Group I ◽  
Group Ii

Background. Nephrotoxicity of cisplatin has been recognized since its introduction more than 25 years ago. However, despite intense efforts to develop less toxic and equally effective alternatives, cisplatin continues to be widely prescribed. Aim and Objectives. The study is aimed at assessing the possible prophylactic effect of coenzyme Q10 (CoQ10) and alpha-lipoic acid (ALA) (separately or in combination) on experimentally cisplatin-induced nephrotoxicity. Subjects and Methods. An experimental study was performed on adult male albino rats (n = 40), weighing 200–250 g. Rats were randomly divided into 5 groups: group I (normal saline control), group II (cisplatin control), group III (CoQ10 and cisplatin), group IV (ALA and cisplatin), and group V (CoQ10, ALA, and cisplatin). CoQ10 and/or ALA were given as pretreatment for 9 days, followed by cisplatin injection in the 10th day of the study, followed by a short posttreatment course for 3 days. Renal functions, tissue antioxidant activity, and inflammatory markers (tumor necrosis factor, TNF) were estimated along with histopathological study. Results. Renal function tests and urinary proteins were significantly higher within group II compared with other groups (P value <0.001). Creatinine clearance was significantly higher with combination therapy (group V compared to other groups). Both TNF and malondialdehyde (MDA) were significantly higher within group II whereas GSH content, catalase, and superoxide dismutase (SOD) were significantly lower in group II. MDA level was significantly lower when combination therapy was used. Marked renal damage was histologically detected in the cisplatin group, whereas the least renal damage was noticed in the combination group. Conclusion. The study confirmed the role of antioxidants in preventing nephrotoxicity caused by cisplatin; the prophylactic effect of combined therapy with CoQ10 and ALA is superior to that of monotherapy.

scholarly journals The effect of vitamin C on Endosulfan-induced oxidative stress parameters in prepubertal rats

Biomedicine ◽  
2020 ◽  
Vol 39 (2) ◽  
pp. 333-338
Author(s):  
Kalaivani Manokaran ◽  
Vasanthalaxmi Krishnananda Rao ◽  
Nilima . ◽  
Manjula Shimoga Durgoji Rao ◽  
Sucheta Prasanna Kumar
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Wistar Rats ◽  
Statistical Significance ◽  
Treated Group ◽  
Reproductive Organs ◽  
Protective Role ◽  
Control Group ◽  
Group I ◽  
Testicular Weight

Introduction and Aim: Oxidative stress plays a very important role in endosulfan-induced toxic effects on reproductive organs. Vitamin C is a potent antioxidant which plays an important role in decreasing oxidative stress. The present study was aimed to investigate the protective role of vitamin C against endosulfan-induced testicular toxicity in Wistar rats. To investigate a protective effect of vitamin C against endosulfan induced toxicity on biochemical changes. Materials and Methods: Seventy male neonatal Wistar rats were divided into  seven groups. The group  I was taken as the control group, the endosulfan-treated were grouped into II (3 mg/kg body weight (BW) and group III (6 mg/kg BW), Group IV (9 mg/kg BW) and Group V (12 mg/kg BW). Group VI (9 mg/kg BW) and group VII (12 mg/kg BW) were pretreated with vitamin C (20 mg/kg BW) for 60 days. After  the experimental procedures, the testicular weight, lactate dehydrogenase (LDH) enzyme and testosterone in plasma, LDH, steroidogenic enzymes 3?-HSD and 17?-HSD in testis were evaluated. One-way ANOVA was used to determine the statistical significance. Results: Significant improvement in the testicular weight (P<0.05) , LDH (P<0.05) levels both in plasma and testis, increase in testosterone(P<0.001) and steroidogenic enzyme levels(P<0.001) was observed in the group pretreated with vitamin C treated group when compared to the endosulfan treated group. Conclusion: Vitamin C decreases the toxic effect of endosulfan on testis. The present action might be  due to its antioxidative properties.

scholarly journals The Protective Role of Prenatal Alpha Lipoic Acid Supplementation against Pancreatic Oxidative Damage in Offspring of Valproic Acid-Treated Rats: Histological and Molecular Study

Biology ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 239
Author(s):  
Fatma M. Ghoneim ◽  
Hani Alrefai ◽  
Ayman Z. Elsamanoudy ◽  
Salwa M. Abo El-khair ◽  
Hanaa A. Khalaf
Keyword(s):  
Oxidative Stress ◽  
Oxidative Damage ◽  
Lipoic Acid ◽  
Caspase 3 ◽  
Protective Role ◽  
Alpha Lipoic Acid ◽  
Group Iii ◽  
Rat Offspring ◽  
Group I

Background: Sodium valproate (VPA) is an antiepileptic drug (AED) licensed for epilepsy and used during pregnancy in various indications. Alpha-lipoic acid (ALA) is a natural compound inducing endogenous antioxidant production. Our study aimed to investigate the effect of prenatal administration of VPA on the pancreas of rat offspring and assess the potential protective role of ALA co-administration during pregnancy. Methods: Twenty-eight pregnant female albino rats were divided into four groups: group I (negative control), group II (positive control, ALA treated), group III (VPA-treated), and group IV (VPA-ALA-treated). The pancreases of the rat offspring were removed at the fourth week postpartum and prepared for histological, immune-histochemical, morphometric, molecular, and oxidative stress marker studies. Results: In group III, there were pyknotic nuclei, vacuolated cytoplasm with ballooning of acinar, α, and β cells of the pancreas. Ultrastructural degeneration of cytoplasmic organelles was detected. Additionally, there was a significant increase in oxidative stress, a decrease in insulin-positive cell percentage, and an increase in glucagon positive cells in comparison to control groups. Moreover, VPA increased the gene expression of an apoptotic marker, caspase-3, with a decrease in anti-apoptotic Bcl2 and nuclear factor erythroid 2-related factor 2 (Nrf2) transcriptional factor. Conversely, ALA improved oxidative stress and apoptosis in group VI, and a consequent improvement of the histological and ultrastructure picture was detected. Conclusion: ALA co-administration with VPA significantly improved the oxidative stress condition, histological and morphometric picture of the pancreas, and restored normal expression of related genes, including Nrf2, caspase-3, and Bcl-2. Administration of α-lipoic acid has a protective effect against VPA-induced pancreatic oxidative damage via its cytoprotective antioxidant effect.

scholarly journals The Effect of 600 mg Alpha-lipoic Acid Supplementation on Oxidative Stress, Inflammation, and RAGE in Older Adults with Type 2 Diabetes Mellitus

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Víctor Manuel Mendoza-Núñez ◽  
Beatriz Isabel García-Martínez ◽  
Juana Rosado-Pérez ◽  
Edelmiro Santiago-Osorio ◽  
José Pedraza-Chaverri ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Older Adults ◽  
Lipoic Acid ◽  
Glycosylated Hemoglobin ◽  
Total Antioxidant Status ◽  
Control Group ◽  
Alpha Lipoic Acid ◽  
Markers Of Inflammation

Alpha-lipoic acid (ALA) has been used as a dietary supplement at different doses in patients with diabetes mellitus type 2 (T2DM) due to its antioxidant, anti-inflammatory, and hypoglycemic effects. However, the reports on the effects of ALA are controversial. For this reason, the purpose of the present study was to determine the effect of 600 mg/day of ALA on the markers of oxidative stress (OxS) and inflammation and RAGE in older adults with T2DM. A quasiexperimental study was carried out with a sample of 135 sedentary subjects (98 women and 37 men) with a mean age of64±1years, who all had T2DM. The sample was divided into three groups: (i) experimental group (EG) with 50 subjects, (ii) placebo group (PG) with 50 subjects, and control group (CG) with 35 subjects. We obtained the following measurements in all subjects (pre- and posttreatment): glycosylated hemoglobin (HbA1c), receptor for advanced glycation end products (RAGE), 8-isoprostane, superoxide dismutase (SOD), glutathione peroxidase (GPx), total antioxidant status (TAS), and inflammatory (CRP, TNF-α, IL-6, IL-8, and IL-10) markers. Regarding the effect of ALA on HbA1c, a decrease was observed in the EG (baseline8.9±0.2vs. posttreatment8.6±0.3) and the PG (baseline8.8±0.2vs. posttreatment8.4±0.3) compared to the CG (baseline8.8±0.3vs. six months9.1±0.3) although the difference was not statistically significant (p<0.05). There was a statistically significant decrease (p<0.05) in the blood concentration of 8-isoprostane in the EG and PG with respect to the CG (EG: baseline100±3vs. posttreatment57±3, PG: baseline106±7vs. posttreatment77±5, and CG: baseline94±10vs. six months107±11pg/mL). Likewise, a statistically significant decrease (p<0.05) in the concentration of the RAGE was found in the EG (baseline1636±88vs. posttreatment1144±68) and the PG (baseline1506±97vs. posttreatment1016±82) compared to CG (baseline1407±112vs. six months1506±128). A statistically significant decrease was also observed in all markers of inflammation and in the activity of SOD and GPx in the CG with respect to the EG and PG. Our findings suggest that the administration of ALA at a dose of 600 mg/day for six months has a similar effect to that of placebo on oxidative stress, inflammation, and RAGE in older adults with T2DM. Therefore, higher doses of ALA should be tried to have this effect. This trial is registered with trial registration numberISRCTN13159380.

scholarly journals Association of Oxidative Stress Markers with Vascular Stiffness Parameters in Patients with Diabetic Neuropathy

BioMed ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 1-12
Author(s):  
Dominika Mačáková ◽  
Markéta Plchová ◽  
Lubica Cibičková ◽  
Ondřej Krystyník ◽  
David Karásek ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetic Neuropathy ◽  
Lipoic Acid ◽  
Vascular Stiffness ◽  
Infusion Therapy ◽  
Control Group ◽  
Alpha Lipoic Acid ◽  
Complications Of Diabetes ◽  
Oxidative Markers ◽  
Wall Stiffness

Introduction: One of the most common chronic complications of diabetes mellitus is diabetic neuropathy. The aim of the study was to elucidate the association between selected markers of oxidative stress and markers of vascular stiffness and to contribute to the understanding of the pathophysiological links between oxidative stress and micro- and macrovascular complications of diabetes. Methods: We enrolled patients with type 2 DM (n = 49), with moderate to severe diabetic polyneuropathy of lower extremities, and a control group without microvascular complications (n = 29). The neuropathy group received alpha-lipoic acid infusion therapy. Sampling was performed before and after treatment to determine the level of oxidative markers (advanced glycation end-products—AGEs, glycation products of AOPP proteins, MDA malondialdehyde and oxidized LDL), parameters of metabolic control and parameters of vascular wall stiffness were measured by sphygmomanometry. Results: After the administration of alpha-lipoic acid, we demonstrated a significant reduction in the level of three selected oxidation markers (AOPP: p < 0.001, AGE: p < 0.001, oxLDL: p < 0.05). In contrast, the level of MDA did not change significantly (p = 0.83). Throughout the group, oxLDL was significantly correlated with central BP (SBP and DBP in the aorta, p < 0.05 and <0.01) and with the augmentation index (AiX/75 bpm, p < 0.01). AOPP significantly correlated with systolic BP in the aorta (p < 0.05). We did not find significant associations in the remaining oxidation markers. Conclusion: In our study, we demonstrated a reduction in the level of oxidative markers after alpha-lipoic acid administration and also an association between markers of oxidative damage to lipids and proteins (oxLDL and AOPP) and some parameters of vascular stiffness.

scholarly journals Amelioration of oxidative stress in broilers during summer

2010 ◽  
Vol 26 (5-6) ◽  
pp. 361-381
Author(s):  
V. Sujatha ◽  
Pandurang Korde ◽  
S.K. Rastogi ◽  
A.K. Madan ◽  
S. Maini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Vitamin C ◽  
Antioxidant Enzyme ◽  
Plasma Glucose ◽  
Total Protein ◽  
Control Group ◽  
Broiler Chicks ◽  
Synthetic Vitamin ◽  
Group I ◽  
Feed Premix

A comparative study on antistressor and antioxidative effects of synthetic vitamin C and polyherbal feed premix Ayucee supplementation in broilers was conducted during the summer months of June-July when the mean temperature-humidity index was 84.74?2.51. Day old broiler chicks (n =60) were randomly divided into three groups. Control group I was given basal diet and treatment groups (II&III) were supplemented with Synthetic vitamin C (100g/tonne of feed) and Ayucee (100g/tonne of feed) from day 0 to six weeks of age. Biochemical parameters were analysed after 3rd & 5th week & erythrocytic antioxidant enzymes were analysed after 3rd & 6th week of experiment. Hormonal & immunological parameters were analysed after 6th of experimental study. After 3rd week, mean plasma glucose, cholesterol & antioxidant enzyme glutathione reductase (GSSG) were significantly (P? 0.01) lower in treated groups (II & III) than control (I); however total protein, albumin to globulin ratio & antioxidant enzyme superoxide dismutase (SOD) were significantly (P? 0.05) different in group II & III compared to group I. After 5th week, mean plasma glucose, total protein, albumin globulin ratio were significantly (P? 0.05) different in both the treatments compared to control. Erythrocytic GSSG were significantly (P? 0.05) different in both the treatments than control, as observed after 6th week. Stress hormones namely cortisol & thyroxine (T4) were observed to be significantly (P?0.05) higher in untreated control than the treated groups. However, the two treatments did not differ significantly. Mean total immunoglobulin (Ig) level was significantly (P?0.01) higher in AYUCEE & vit-C treated birds than control after 6th week of study. It can be concluded from the results that oxidative stress in broilers during summer could be ameliorated using antioxidant synthetic vitamin C & the polyherbal antistressor, immunomodulator & adaptogenic feed premix AYUCEE.

scholarly journals Protective Effect of Alpha-Lipoic Acid on Salivary Dysfunction in a Mouse Model of Radioiodine Therapy-Induced Sialoadenitis

2020 ◽  
Vol 21 (11) ◽  
pp. 4136 ◽  
Author(s):  
Jung Hwa Jung ◽  
Jin Hyun Kim ◽  
Myeong Hee Jung ◽  
Seung Won Kim ◽  
Bae Kwon Jeong ◽  
...  
Keyword(s):  
Body Weight ◽  
Mouse Model ◽  
Lipoic Acid ◽  
Single Photon ◽  
Photon Emission ◽  
Control Group ◽  
Emission Computed Tomography ◽  
Alpha Lipoic Acid ◽  
99Mtc Pertechnetate ◽  
Group I

Radioiodine (RI) therapy is known to cause salivary gland (SG) dysfunction. The effects of antioxidants on RI-induced SG damage have not been well described. This study was performed to investigate the radioprotective effects of alpha lipoic acid (ALA) administered prior to RI therapy in a mouse model of RI-induced sialadenitis. Four-week-old female C57BL/6 mice were divided into four groups (n = 10 per group): group I, normal control; group II, ALA alone (100 mg/kg); group III, RI alone (0.01 mCi/g body weight, orally); and group IV, ALA + RI (ALA at 100 mg/kg, 24 h and 30 min before RI exposure at 0.01 mCi/g body weight). The animals in these groups were divided into two subgroups and euthanized at 30 or 90 days post-RI treatment. Changes in salivary 99mTc pertechnetate uptake and excretion were tracked by single-photon emission computed tomography. Salivary histological examinations and TUNEL assays were performed. The 99mTc pertechnetate excretion level recovered in the ALA treatment group. Salivary epithelial (aquaporin 5) cells of the ALA + RI group were protected from RI damage. The ALA + RI group exhibited more mucin-containing parenchyma and less fibrotic tissues than the RI only group. Fewer apoptotic cells were observed in the ALA + RI group compared to the RI only group. Pretreatment with ALA before RI therapy is potentially beneficial in protecting against RI-induced salivary dysfunction.

scholarly journals RUTIN RESTORE BIOCHEMICAL CHANGES, OXIDATIVE STRESS AND BETATROPHIN LEVEL IN STZ-INDUCED DIABETIC RATS

2019 ◽  
pp. 62-70
Author(s):  
AL-SHIMAA M. ABAS ◽  
AHMED F. EL-FARAFG ◽  
NEHAL W. ABDALLA
Keyword(s):  
Oxidative Stress ◽  
Histopathological Examination ◽  
Experimental Period ◽  
Positive Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Standard Group ◽  
Group V ◽  
Group I

Objective: Diabetes mellitus (DM) is associated with long-term damage, dysfunction, of various organs. Study aims to assessrole of rutin on experimentally induced diabetes. Methods: 50 adult male albino rats divided into 5 groups. Group I (control group, rats were orally administered with 1 ml saline daily). Group II (DMSO group, rats were orally administered with 0.2 % DMSO for 60 d orally). Group III (positive control, animals were injected intraperitoneally with 60 mg/kg b. wtstreptozotocin followed by intraperitoneal injection with 120 mg/kg b. wt of Nicotinamide after 15 min). Group IV (therapeutic group, diabetic rats treated with 100 mg/kg b. wt of rutin for 60 d orally). Group V (standard group, diabetic animals treated with 100 mg/kg b. wt of metformin for 60 d orally). At the end of the experimental period blood serum and plasma, liver, kidney and pancreatic tissues were collected. Results: Diabetic rats showed a significant increase in plasma glucose, serum urea, creatinine, cholesterol and triglyceride. Also, induced oxidative stress as pointed out an increase in MDA level, decrease in GSH level, GST and CAT activities in compared to control group. Also, showed an increase in plasma and tissues levels of betatrophin. Oral administration of rutin cause decrease in elevated biochemical and oxidative stress parameters. Also, decrease betatrophin level when compared with diabetic rats. Our results were confirmed by histopathological examination of different tissues. Conclusion: This study suggests thatrutinexihibitsantihyperglycemic and antioxidant activity in streptozotocin-induced diabetic rats.

scholarly journals Effects of Alpha Lipoic Acid Supplementation on Serum Levels of Oxidative Stress, Inflammatory Markers and Clinical Prognosis among Acute Ischemic Stroke Patients: A Randomized, Double Blind, TNS Trial

2020 ◽  
Vol 10 (2) ◽  
pp. 284-289
Author(s):  
Sheyda Shaafi ◽  
Soraiya Ebrahimpour-Koujan ◽  
Mohammad Khalili ◽  
Seyad Morteza Shamshirgaran ◽  
Mazyar Hashemilar ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipoic Acid ◽  
Serum Levels ◽  
Control Group ◽  
Controlled Clinical Trial ◽  
Alpha Lipoic Acid ◽  
Randomized Controlled Clinical Trial ◽  
Stroke Patients ◽  
Randomized Controlled ◽  
Tnf Α

Purpose: Stroke is one of the most common conditions causing death. There have been few studies examining the effects of alpha lipoic acid (ALA) on stroke patients. In this regard, the present randomized controlled clinical trial was conducted to examine the effects of ALA supplementation on serum albumin, and inflammatory and oxidative stress markers in stroke patients. Methods: The present paralleled randomized controlled clinical trial involved 42 stroke patients who were over 40 years and under enteral feeding. The participants were randomly assigned into two groups and finally 40 patients completed the study. Patients in alpha lipoic acid group (n=19) took 1200 mg ALA supplement daily along with their meal, and participants in control group (n=21) underwent the routine hospital diet for 3 weeks. Fasting blood samples were obtained and albumin, oxidative stress, and inflammatory indices were assessed at baseline, as well as at the end of the trial. Results: After 3 weeks, treatment of patients with ALA led to a significant decrease in tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) levels (P=0.01) compared to baseline. But serum levels of albumin, total antioxidant capacity (TAC), malondialdehyde (MDA), highsensitivity C-reactive protein (hs-CRP), IL-6 and TNF-α did not change significantly vs. control group (P>0.05). Conclusion: ALA did not significantly change the serum levels of albumin and inflammatory as well as antioxidant capacity indices in stroke patients compared with the control group. More clinical trials with large sample sizes and long duration are needed to clarify the effects of ALA on these patients.

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