scholarly journals The Potential CircRNAs in Imatinib Resistance of GIST

2021 ◽  
Author(s):  
Jingyi Yan ◽  
Xiaolei Chen ◽  
Qiantong Dong ◽  
Ji Lin ◽  
Xuecheng Sun
Keyword(s):  
Resistance Mechanism ◽  
Circular Rna ◽  
Circular Rnas ◽  
Imatinib Resistance ◽  
Secondary Resistance ◽  
Primary Gist ◽  
Kegg Pathway Analysis ◽  
Tumor Tissues ◽  
Competing Endogenous Rna Network ◽  
The Relationship

Abstract Background: Recent studies have found that circular RNA is an abundant RNA species, belongs to part of the competing endogenous RNA network(ceRNA), which was proved to play an important role in the development, diagnosis and progress of diseases.Methods: We determined the expression of circular RNAs in paired normal gastric tissues(N), primary GIST (gastrointestinal stromal tumor) tissues (Y or YC) and imatinib mesylate secondary resistance GIST tissues(C) with microarray and predicted 8677 dysregulated circular RNAs.Results: We identified 15 circRNAs was up-regulated and 8 circRNAs were down-regulated in C group. Gene ontology (GO)and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that these host linear transcripts that differentially express circular RNAs are involved in many key biological pathways., predicting the potential tumor-genesis and drug resistance mechanism was related with HIF-1 pathway, later we draw the cirRNA-miRNA-mRNA network involved in the HIF-1 pathway, found that several dysregulated circRNAs and the relationship between circRNA-miRNAs-mRNA, such as circRNA_06551, circRNA_14668, circRNA_04497, circRNA_08683, circRNA_09923(Green, down-regulation) and circRNA_23636, circRNA_15734(Red, up-regulation). Conclusions: Taken together, we identified a panel of dysregulated circRNAs that may be potential biomarkers even therapy relevant to the GIST, especially imatinib secondary resistance GIST.

scholarly journals The Potential circRNAs in Imatinib Resistance of GIST

2021 ◽  
Author(s):  
Jingyi Yan ◽  
Xiaolei Chen ◽  
Qiantong Dong ◽  
Ji Lin ◽  
Xuecheng Sun
Keyword(s):  
Critical Role ◽  
Resistance Mechanism ◽  
Circular Rnas ◽  
Imatinib Resistance ◽  
Secondary Resistance ◽  
Primary Gist ◽  
Kegg Pathway Analysis ◽  
Tumor Tissues ◽  
Competing Endogenous Rna Network ◽  
The Relationship

Abstract Background: Circular RNAs are a recently (re-)discovered abundant RNA species, belongs to part of the competing endogenous RNA network(ceRNA), Which was proved to play a critical role in the development, diagnosis and progress of diseases. Methods: We analyzed the expression of circular RNAs in paired normal gastric tissues(N), primary GIST (gastrointestinal stromal tumor) tissues (Y or YC) and imatinib mesylate secondary resistance GIST tissues(C) with microarray and predicted 8677 dysregulated circular RNAs. Results: We identified 15 circRNAs was up-regulated and 8 circRNAs were down-regulated in C group. Gene ontology (GO)and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified that the host linear transcripts of these differentially expressed circRNAs were involved in many critical biological pathways and molecular functions, predicting the potential tumor-genesis and drug resistance mechanism was related with HIF-1 pathway, later we draw the cirRNA-miRNA-mRNA network involved in the HIF-1 pathway, found that several dysregulated circRNAs and the relationship between circRNA-miRNAs-mRNA, such as circRNA_06551, circRNA_14668, circRNA_04497, circRNA_08683, circRNA_09923(Green, down-regulation) and circRNA_23636, circRNA_15734(Red, up-regulation). Conclusions: Taken together, we identified a panel of dysregulated circRNAs that may be potential biomarkers even therapy relevant to the GIST, especially imatinib secondary resistance GIST.

scholarly journals Correlation of Circular RNA hsa_circ_0001946, hsa_circ_0125589, and Environmental Factors With Hypertension

2020 ◽  
Vol 33 (11) ◽  
pp. 1047-1047
Author(s):  
Wan-yue Liu ◽  
Yi Sun ◽  
Shu-na Huang ◽  
Yu-zhen Lin ◽  
Hong-yan Guo ◽  
...  
Keyword(s):  
Environmental Factors ◽  
Peripheral Blood ◽  
Circular Rna ◽  
Peripheral Blood Lymphocytes ◽  
Normal Weight ◽  
Circular Rnas ◽  
Blood Leukocytes ◽  
Crossover Analysis ◽  
History Of ◽  
The Relationship

Abstract Background To investigate the main environmental factors of hypertension and the relationship between hypertension and circular RNAs in peripheral blood lymphocytes. Methods This was a case–control study. A total of 681 hypertension patients and 485 subjects without hypertension were recruited between April 2017 and October 2018. All participations completed the questionnaire investigation, physical examination, and laboratory detection. Quantitative real-time polymerase chain reaction was used to analyze circRNAs (hsa_circ_0001946 and hsa_circ_0125589) in peripheral blood leukocytes in 84 hypertensives and 84 controls. Multivariate logistic regression and crossover analysis were used to analyze the interaction and association between environmental factors and circRNAs in hypertension. Results After adjusted by gender, age and marital status, overweight/obesity (odds ratio (OR) = 1.66, 95% confidence interval (CI) 1.24–2.22), abdominal obesity (OR = 2.17, 95% CI 1.54–3.04), anxiety (OR = 2.15, 95% CI 1.41–3.28), family history of hypertension (OR = 4.26, 95% CI 3.18–5.70), and higher levels of hsa_circ_0001946 (OR = 4.13, 95% CI 1.85–9.21) were risk factors for hypertension, while levels of hsa_circ_0125589 were not associated with hypertension. Crossover analysis showed that the risk of hypertension was 13.12 times higher (95% CI 3.89–44.23) in overweight subjects with high hsa_circ_0001946 levels compared with normal weight subjects with low hsa_circ_0001946 levels. Further, the risk of hypertension was 17.78 times higher (95% CI 1.88–168.61) in subjects with anxiety and high hsa_circ_0001946 levels. Conclusions Hypertension is the result of both environmental factors and genetic factors. Higher hsa_circ_0001946 levels, overweight and anxiety may increase the risk of hypertension, while hsa_circ_0125589 levels are not related to hypertension.

scholarly journals A novel circular RNA, circXPO1, promotes lung adenocarcinoma progression by interacting with IGF2BP1

2020 ◽  
Vol 11 (12) ◽  
Author(s):  
Qi Huang ◽  
Haifa Guo ◽  
Shaodong Wang ◽  
Yi Ma ◽  
Haiming Chen ◽  
...  
Keyword(s):  
Lung Adenocarcinoma ◽  
Therapeutic Target ◽  
Noncoding Rnas ◽  
Xenograft Model ◽  
Gene Copy Number ◽  
Circular Rna ◽  
Gene Copy ◽  
Circular Rnas ◽  
Functional Roles ◽  
Tumor Tissues

AbstractStudies have demonstrated that noncoding RNAs play important roles in various types of cancer; however, noncoding RNAs derived from regions of genomic alterations have rarely been explored, especially for circular RNAs (circRNA). Previously, we found several circRNAs were upregulated in lung adenocarcinoma (LUAD) tumor tissues by RNA sequencing. Here, we characterized a novel circRNA, circXPO1, in LUAD, which is derived from a well-established cancer therapeutic target, XPO1. circXPO1, is formed by back-splicing of exon 3 and exon 4 of XPO1 gene. circXPO1 was highly expressed in LUAD tissues compared with paired adjacent non-tumor tissues, and high circXPO1 expression correlated with worse overall survival. circXPO1 expression was positively correlated with the XPO1 gene copy number. Mechanically, circXPO1 could bind with IGF2BP1 and enhance CTNNB1 mRNA stability, and subsequently promote LUAD progression. In a LUAD patient-derived xenograft model, intratumoural injection of cholesterol-conjugated siRNA specifically targeting circXPO1 efficiently suppressed tumor growth. To summary, these results suggest that circXPO1 is critical for LUAD progression and may serve as a biomarker for poor prognosis and a therapeutic target. On the other hand, the functional roles of noncoding transcripts derived from coding genes should be re-evaluated.

scholarly journals Circular RNA-ITCH Suppresses Lung Cancer Proliferation via Inhibiting the Wnt/β-Catenin Pathway

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Li Wan ◽  
Lin Zhang ◽  
Kai Fan ◽  
Zai-Xing Cheng ◽  
Quan-Chao Sun ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Progression ◽  
Ectopic Expression ◽  
Circular Rna ◽  
Circular Rnas ◽  
Parental Cancer ◽  
Cancer Proliferation ◽  
Mirna Sponge ◽  
Tumor Tissues ◽  
Cancer Tissues

As a special form of noncoding RNAs, circular RNAs (circRNAs) played important roles in regulating cancer progression mainly by functioning as miRNA sponge. While the function of circular RNA-ITCH (cir-ITCH) in lung cancer is still less reported, in this study, we firstly detected the expression ofcir-ITCHin tumor tissues and paired adjacent noncancer tissues of 78 patients with lung cancer using a TaqMan-based quantitative real-time PCR (qRT-PCR). The results showed that the expression ofcir-ITCHwas significantly decreased in lung cancer tissues. In cellular studies,cir-ITCHwas also enhanced in different lung cancer cell lines, A549 and NIC-H460. Ectopic expression ofcir-ITCHmarkedly elevated its parental cancer-suppressive gene, ITCH, expression and inhibited proliferation of lung cancer cells. Molecular analysis further revealed thatcir-ITCHacted as sponge of oncogenic miR-7 and miR-214 to enhance ITCH expression and thus suppressed the activation of Wnt/β-catenin signaling. Altogether, our results suggested thatcir-ITCHmay play an inhibitory role in lung cancer progression by enhancing its parental gene, ITCH, expression.

scholarly journals Exosomal circRNAs: new players in the field of cholangiocarcinoma

2019 ◽  
Vol 133 (21) ◽  
pp. 2239-2244 ◽  
Author(s):  
Corentin Louis ◽  
Matthis Desoteux ◽  
Cédric Coulouarn
Keyword(s):  
Cell Lines ◽  
Molecular Mechanisms ◽  
Human Tumor ◽  
Direct Interaction ◽  
Circular Rna ◽  
Circular Rnas ◽  
Loss Of Function ◽  
Non Invasive ◽  
Tumor Tissues

Abstract Cholangiocarcinoma (CCA) is a deadly cancer worldwide associated with limited therapeutic options. A recent study published in Clinical Science by Wang and colleagues [Clin. Sci. (2019) 133(18), 1935–1953] brought new perspectives to CCA management and therapy by focusing on circular RNAs (circRNAs). CircRNAs belong to an emerging class of functional non-coding RNAs (ncRNAs) regulating numerous biological processes. Notably, circRNAs have been associated with cancer onset and progression, although reports in CCA are very limited so far. In this work, the expression of circular RNA circ-0000284 (aka circHIPK3) was specifically elevated in CCA cell lines, human tumor tissues and plasma exosomes. Gain and loss of function approaches were performed to better understand the molecular mechanisms regulated by circ-0000284. Notably, the authors evaluated the role of circ-0000284 as a microRNA (miRNA) sponge. By prediction analysis and functional tests, a direct interaction was demonstrated with miR-637 that targets lymphocyte antigen-6 E (LY6E). Increased expression of circ-0000284 was associated with enhanced migration, invasion and proliferation of CCA cell lines. Interestingly, exosomal-mediated circ-0000284 was reported to exhibit pro-oncogenic effects on surrounding normal cells. Altogether, these data highlight circRNAs not only as new players in CCA pathogenesis but also as promising molecules for innovative non-invasive biomarkers, as circRNAs are enriched and stable in exosomes. Further investigations on extracellular vesicles should provide the necessary tools to improve CCA diagnosis, and move toward targeted-therapies.

scholarly journals Circular RNA: biogenesis, degradation, functions and potential roles in mediating resistance to anticarcinogens

Epigenomics ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 267-283 ◽  
Author(s):  
Xiuchao Geng ◽  
Youchao Jia ◽  
Yuhao Zhang ◽  
Liang Shi ◽  
Qiang Li ◽  
...  
Keyword(s):  
Noncoding Rnas ◽  
Circular Rna ◽  
Cancer Drug ◽  
Circular Rnas ◽  
Cancer Drug Resistance ◽  
Cancer Pathogenesis ◽  
Scientific Papers ◽  
Rna Biogenesis ◽  
And Function ◽  
The Relationship

Aim: This review aims to systematically describe the biogenesis and degradation of circular RNAs (circRNAs), discusses the major functions of circRNAs, introduces the mechanisms by which circRNAs play a role in cancer, comprehensively summarize the relationship between circRNAs and anticarcinogen resistance as well as underlying specific mechanisms in multiple cancers. Materials & methods: We screened and analyzed large quantity of scientific papers which associated with circRNAs, noncoding RNAs, function, cancer, drug resistance and chemoresistance, and then summarized in Figures 1 & 2 & Table 1. Results & conclusion: The biogenesis, degradation and function of circRNAs are specially compared with other noncoding RNAs, it can affect cancer pathogenesis and progression and are implicated in mediating resistance to various anticarcinogens in various types of cancer.

scholarly journals Cancer-related circular RNA: diverse biological functions

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Dan Cheng ◽  
Jing Wang ◽  
Zigang Dong ◽  
Xiang Li
Keyword(s):  
Noncoding Rnas ◽  
Circular Rna ◽  
Long Noncoding Rnas ◽  
Circular Rnas ◽  
Biological Functions ◽  
Diagnosis Of Cancer ◽  
The Relationship ◽  
Potential Biomarkers

AbstractNoncoding RNAs, including long noncoding RNAs (lncRNAs), microRNAs (miRNAs) and circular RNAs (circRNAs), are involved in regulating biological functions. In recent decades, miRNAs and lncRNAs have both inspired a wave of research, but the study of circRNA functions is still in its infancy. Studies have found that circRNAs actively participate in the occurrence and development of various diseases, which emphasizes the importance of circRNAs. Here, we review the features and classification of circRNAs and summarize their functions. Then, we briefly describe how to analyze circRNAs by bioinformatics procedures. In addition, the relationship between circRNAs and cancers is discussed with an emphasis on proving whether circRNAs can be potential biomarkers for the prognosis and diagnosis of cancer.

scholarly journals Circular RNAs in the Regulation of Oxidative Stress

2021 ◽  
Vol 12 ◽  
Author(s):  
Yao Zhang ◽  
Yu Chen ◽  
Yue Wan ◽  
Yueshui Zhao ◽  
Qinglian Wen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Science And Technology ◽  
Circular Rna ◽  
Reactive Metabolites ◽  
Circular Rnas ◽  
Stress System ◽  
The Past ◽  
The Relationship

Oxidative stress caused by an imbalance between the production and elimination of reactive metabolites and free radicals can lead to the development of a variety of diseases. Over the past years, with the development of science and technology, circular RNA (circRNA) has been found to be closely associated with oxidative stress, which plays an important role in the process of oxidative stress. Currently, the understanding of circRNAs in the mechanism of oxidative stress is limited. In this review, we described the relationship between oxidative stress and circRNAs, the circRNAs related to oxidative stress, and the role of circRNAs in promoting or inhibiting the occurrence and development of diseases associated with the oxidative stress system.

scholarly journals Genome-wide identification and characterization of circular RNA m6A modification in pancreatic cancer

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Ying Ye ◽  
Weiyi Feng ◽  
Jialiang Zhang ◽  
Kaiyu Zhu ◽  
Xudong Huang ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Circular Rna ◽  
Ductal Adenocarcinoma ◽  
Circular Rnas ◽  
Tissue Samples ◽  
Normal Tissues ◽  
Genome Wide ◽  
Genomic Landscape ◽  
Tumor Tissues

Abstract Background N6-methyladenosine (m6A) is the most abundant modification of RNA in eukaryotic cells and play critical roles in cancer. While most related studies focus on m6A modifications in linear RNA, transcriptome-wide profiling and exploration of m6A modification in circular RNAs in cancer is still lacking. Methods For the detection of m6A modification in circRNAs, we developed a new bioinformatics tools called Circm6A and applied it to the m6A-seq data of 77 tissue samples from 58 individuals with pancreatic ductal adenocarcinoma (PDAC). Results Circm6A performs better than the existing circRNA identification tools, which achieved highest F1 score among these tools in the detection of circRNAs with m6A modifications. By using Circm6A, we identified a total of 8807 m6A-circRNAs from our m6A-seq data. The m6A-circRNAs tend to be hypermethylated in PDAC tumor tissues compared with normal tissues. The hypermethylated m6A-circRNAs were associated with a significant gain of circRNA-mRNA coexpression network, leading to the dysregulation of many important cancer-related pathways. Moreover, we found the cues that hypermethylated m6A-circRNAs may promote the circularization and translation of circRNAs. Conclusions These comprehensive findings further bridged the knowledge gaps between m6A modification and circRNAs fields by depicting the m6A-circRNAs genomic landscape of PDAC patients and revealed the emerging roles played by m6A-circRNAs in pancreatic cancer. Circm6A is available at https://github.com/canceromics/circm6a.

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