scholarly journals ESTIMATES OF POLLEN FERTILITY AND ISOZYME PROFILES OF STAMINATE KIWIFRUIT PLANTS IN SELECTED CALIFORNIA ORCHARDS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 624c-624
Author(s):  
M. L. Meyer ◽  
F. A. Bliss
Keyword(s):  
Pollen Fertility ◽  
Genotypic Variation ◽  
Fruit Size ◽  
Actinidia Deliciosa ◽  
Percentage Germination ◽  
Dioecious Plant ◽  
Positive Correlation ◽  
Number Of Seeds

Kiwifruit (Actinidia deliciosa) is a functionally dioecious plant where fruit size is dependent on number of seeds set. Pollen fertility was estimated in 1990 and 1991 by percentage stainability and percentage germinability in vitro. Profiles of the isozymes AAT, GPI and PGM were used to assess if any large differences in pollen fertility could be attributed to genotypic variation. Based on these three isozymes, eight different genotypes were discovered. Although significant differences were found among vines within orchards and among orchards, all vines can be considered good pollenizers (stainability > 87%). A positive correlation was found in 1991 between percentage stainability and percentage germination.

The Strong Positive Correlation between Factor VII Clotting Activity Using Bovine Thromboplastin and the Activated Factor VII Level

1995 ◽  
Vol 73 (03) ◽  
pp. 429-434 ◽  
Author(s):  
Kazuomi Kario ◽  
Takefumi Matsuo ◽  
Reiko Asada ◽  
Toshiyuki Sakata ◽  
Hisao Kato ◽  
...  
Keyword(s):  
Risk Factor ◽  
Bovine Brain ◽  
Factor Vii ◽  
Rabbit Brain ◽  
Strong Positive Correlation ◽  
Chromogenic Substrate ◽  
Moderate Correlation ◽  
Positive Correlation ◽  
Activated Factor Vii

SummaryWe compared factor VII clotting activity (FVIIc) assays using different thromboplastins to determine which is the most sensitive for activated FVII (FVIIa) or for FVII antigen (FVIIag). FVIIc levels were measured using thromboplastins derived from bovine brain (FVIIc Bov), human placenta (FVIIc Hum), and rabbit brain (FVIIc Rab). FVIIa levels were measured by fluorogenic assays using human soluble tissue factor (rsTF) or bovine rsTF. We also measured FVII activity by an amidolytic assay (FVIIc:am Hum) using human thromboplastin and a chromogenic substrate for thrombin. FVIIag levels were determined by ELISA. In the FVIIa assay, the reaction time obtained from using bovine rsTF was shorter than that with human rsTF, suggesting that the interaction of plasma FVIIa with bovine rsTF was stronger than with human rsTF. The plasma FVIIa levels measured using human rsTF and bovine rsTF were almost the same (r=0.947, p<0.0001). Among the three FVIIc assays, FVIIc Bov had the strongest positive correlation with the plasma FVIIa level (r=0.886, p<0.000l), but had no correlation with FVIIag. An increase of 1 ng/ml in the plasma FVIIa level yielded a 27.9% increase of FVIIc Bov. Plasma FVIIc Hum and FVIIc:am Hum showed moderate correlations with both FVIIa (r=0.520, p<0.02 and r=0.569, p<0.01, respectively) and FVIIag (r=0.438, p<0.05 and r=0.468, p<0.05, respectively). FVIIc Rab had the lowest correlation with FVIIa (r=0.367, p<0.1), but had a moderate correlation with FVIIag (r=0.436, p<0.05). After in vitro cold activation, FVIIc Bov levels increased the most and FVIIc:am levels showed the least change. These findings indicate that consideration of the thromboplastin used for assay is necessary when assessing the clinical significance of FVII activity as a cardiovascular risk factor.

IMPACT OF SORBITOL- INDUCED OSMOTIC STRESS ON SOME BIOCHEMICAL TRAITS OF POTATO IN VITRO

2020 ◽  
Vol 51 (4) ◽  
pp. 1038-1047
Author(s):  
Mawia & et al.
Keyword(s):  
Ascorbic Acid ◽  
Osmotic Stress ◽  
Cytoplasmic Membrane ◽  
Genotypic Variation ◽  
Membrane Damage ◽  
Membrane Integrity ◽  
Culture Collection ◽  
Nutritive Medium ◽  
Starting Point

This study had as principal objective identification of osmotic-tolerant potato genotypes by using "in vitro" tissue culture and sorbitol as a stimulating agent, to induce water stress, which was added to the  culture nutritive medium in different concentration (0,50, 110, 220, 330 and 440 mM).  The starting point was represented by plantlets culture collection, belonging to eleven potato genotypes: Barcelona, Nectar, Alison, Jelly, Malice, Nazca, Toronto, Farida, Fabulla, Colomba and Spunta. Plantlets were multiplied between two internodes to obtain microcuttings (in sterile condition), which were inoculated on medium. Sorbitol-induced osmotic stress caused a significant reduction in the ascorbic acid, while the concentration of proline, H2O2 and solutes leakage increased compared with the control. Increased the proline content prevented lipid peroxidation, which played a pivotal role in the maintenance of membrane integrity under osmotic stress conditions. The extent of the cytoplasmic membrane damage depends on osmotic stress severity and the genotypic variation in the maintenance of membranes stability was highly associated with the ability of producing more amounts of osmoprotectants (proline) and the non-enzymic antioxidant ascorbic acid in response to osmotic stress level. The results showed that the genotypes Jelly, Nectar, Allison, Toronto, and Colomba are classified as highly osmotic stress tolerant genotypes, while the genotypes Nazca and Farida are classified as osmotic stress susceptible ones.

Seed-borne fungi of cowpea [Vigna unguiculata (L.) Walp] and their possible control in vitro using locally available fungicides in Botswana.

2016 ◽  
Vol 5 (11) ◽  
pp. 5016 ◽  
Author(s):  
K. B. Khare* ◽  
Loeto D. ◽  
Wale K. ◽  
Salani M.
Keyword(s):  
Seed Germination ◽  
Continuous Light ◽  
Sodium Hypochlorite Solution ◽  
Distilled Water ◽  
Percentage Germination ◽  
Department Of Agriculture ◽  
Hypochlorite Solution ◽  
Cowpea Cultivars ◽  
Petri Plate

Seeds of three cowpea cultivars namely Black eye, ER 7 and Tswana obtained from the Department of Agriculture Research, Gaborone were tested for the presence of seed-borne fungi, and their possible control in vitro using locally available fungicides. Four hundred fifty seeds of each cultivar of cowpea were disinfected with 2% sodium hypochlorite solution for 10 min and washed three times with sterile distilled water before placing them in PDA plates (5 seeds/9 cm Petri plate), incubated at 22±2o C for 12 hour each under continuous light and dark. A total of eight fungi were detected from seeds of cowpea. These were Aspergillus flavus, A. niger, Cylindrocarpon sp., Fusarium equisiti, F. oxyaporum, Penicillium chyrosogenum, Rhizopus oligosporus and R. stolonifer. Rhizopus spp. were dominant fungi recovered from seeds, followed by Penicillium, Aspergillus, Fusarium and Cylindrocarpon. The fungi detected resulted in decay and rotting of seeds, and thereby reducing percentage germination of seeds (22%, 37% and 63 % seed germination in Black eye, ER7 and Tswana varieties of cowpea respectively). Out of four fungicides tested, benlate, captan, dithane M 45 and chlorothalanil. Dithane M45 effectively controlled seed-borne fungi, and enhanced seed germination to an average of 86% (93% germination with no fungi detected in Tswana variety) as compared to chlorothalonile (79%), benlate and captan (77%) and un-treated seeds (45%). The fungal incidence was reduced to 2.3%, 4.3%, 5.3% and 5.3% when seeds were treated with dithane M-45, chlorothalonil, benlate and captan respectively as compared to 62% in non-treated seeds.

VARIATION AMONG BRITISH COLUMBIA AND NORTHERN ALBERTA POPULATIONS OF RASPBERRIES, RUBUS IDAEUS SUBSPP. STRIGOSUS MICHX.

1972 ◽  
Vol 52 (6) ◽  
pp. 1067-1072 ◽  
Author(s):  
M. C. J. VAN ADRICHEM
Keyword(s):  
British Columbia ◽  
Seed Weight ◽  
Fruit Weight ◽  
Winter Hardiness ◽  
Rubus Idaeus ◽  
Specific Character ◽  
Positive Correlation ◽  
Total Seed ◽  
Northern Alberta ◽  
Number Of Seeds

Several population samples of Rubus idaeus subspp. strigosus Michx. (raspberries) were collected in British Columbia and northern Alberta and a number of characters were studied and compared with the cultivar Trent. Significant differences between populations were found for cane length, number of buds per cane, percentage of buds growing, number of inflorescences and flowers per bud, fruit and seed weight. There was a positive correlation between the total seed weight and the number of seeds, and between the total seed and fruit weight. No distinct types could be established based on location or elevation. None of the populations showed significantly better winter hardiness than the cultivar Trent, and although some had a specific character that was superior, none was found to have overall superiority.

scholarly journals Telomere Length in Norway Spruce during Somatic Embryogenesis and Cryopreservation

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 416
Author(s):  
Tuija Aronen ◽  
Susanna Virta ◽  
Saila Varis
Keyword(s):  
Somatic Embryogenesis ◽  
Telomere Length ◽  
Norway Spruce ◽  
Genotypic Variation ◽  
Production Capacity ◽  
Stress Factors ◽  
Embryo Production ◽  
One Year ◽  
Plant Telomeres

Telomeres i.e., termini of the eukaryotic chromosomes protect chromosomes during DNA replication. Shortening of telomeres, either due to stress or ageing is related to replicative cellular senescence. There is little information on the effect of biotechnological methods, such as tissue culture via somatic embryogenesis (SE) or cryopreservation on plant telomeres, even if these techniques are widely applied. The aim of the present study was to examine telomeres of Norway spruce (Picea abies (L.) Karst.) during SE initiation, proliferation, embryo maturation, and cryopreservation to reveal potential ageing or stress-related effects that could explain variation observed at SE process. Altogether, 33 genotypes from 25 families were studied. SE initiation containing several stress factors cause telomere shortening in Norway spruce. Following initiation, the telomere length of the embryogenic tissues (ETs) and embryos produced remains unchanged up to one year of culture, with remarkable genotypic variation. Being prolonged in vitro culture can, however, shorten the telomeres and should be avoided. This is achieved by successful cryopreservation treatment preserving telomere length. Somatic embryo production capacity of the ETs was observed to vary a lot not only among the genotypes, but also from one timepoint to another. No connection between embryo production and telomere length was found, so this variation remains unexplained.

scholarly journals Evaluation of tear production with modified Schirmer tear test-1 during the neonatal period in cats

2011 ◽  
Vol 31 (4) ◽  
pp. 350-354 ◽  
Author(s):  
L.F. Conceição ◽  
A.P. Ribeiro ◽  
M.L.M. Torres ◽  
M.L.G. Lourenço ◽  
J.L. Laus
Keyword(s):  
Reference Values ◽  
Neonatal Period ◽  
Positive Correlation ◽  
Tear Secretion ◽  
Tear Production ◽  
Schirmer Tear Test ◽  
Maturity Parameters ◽  
Time Point

The study aimed to evaluate tear production by means of modified Schirmer tear tes-1 (mSTT-1) in neonate cats. Likewise, correlation between mSTT-1 and STT-1 was assessed in vitro. Standard SST strips were cut in half and after eye lid opening, tear production of neonates (n=15) was daily measured in both eyes (mSTT-1), until the 7th day, and at day 14, 21, and 28. Animals were daily weighted until 28 days of age. Results were compared statistically (P<0.05). During the first 7 days, the overall mSTT-1 mean was 0.76 wetting/minute. Significant differences between right and left eyes were not observed at any time point (P=1.00). Tear secretion increased significantly, from the 14th to 28th day, in comparison with 7 first days (P<0.05). Positive correlation between maturity parameters and tear secretion was observed (P<0.0001). Distance between slopes of each strip changed significantly (P<0.0001). It was concluded that tear secretion in the neonatal period of cats is very below the reference values described for young and adults of the same species. It is not possible to extrapolate results obtained with mSTT-1 to standard STT-1.

Morphological and physiological traits associated with seed yield in different chickpea (Cicer arietinum L.) genotypes under irrigated andwater‐deficit environments

2011 ◽  
Vol 1 (6) ◽  
pp. 260-267
Author(s):  
Reza Talebi ◽  
Ezzat Karami
Keyword(s):  
Chlorophyll Content ◽  
Water Deficit ◽  
Seed Yield ◽  
Seed Weight ◽  
Significant Negative Correlation ◽  
Physiological Traits ◽  
Positive Correlation ◽  
Chickpea Cultivar ◽  
High Level ◽  
Number Of Seeds

The morpho â€physiological traits associated with seed yield in chickpea (Cicerarietinum L.) were evaluated on thirty five chickpea genotypes under twodifferent water regimes. In optimum condition, the highest correlation wasbelonged to number of seeds/plant and number of pods/plant (0.944**) andin water deficit condition, the strongest correlation of seed yield was observedwith number of seeds/plant (0.875**), number of pods/plant(0.789**) and 100  â€seed weight (0.438**). Physiological traits (RWC, Chlorophylland Carotenoid) indicated low and positive correlation with yield, buthigh positive correlation with other seeds yields related traits in optimumcondition while, under water deficit condition, chlorophyll content and RWChad low and non â€significant negative correlation with seeds yield. Carotenoid,number of seeds/plant and 100 â€seed weight showed more direct positiveeffect on yield in optimum environment and in stress condition, numberof seeds/ plant and 100 â€seed weight showed highest direct effect on seedyield. Results of factor analysis showed that four factors explained 88.54%and 34.5% of the total variance caused in the characters in optimum andwater deficit environment, respectively. In general, the results suggestedthat a chickpea cultivar, for increased yield under non â€stress conditions,should have maximum number of seeds and pods per plant and under stressconditions should have maximum number of seeds and pods per plant andalso keep the high level of RWC and chlorophyll content in their leaves. Thus,identifying these traits as selection criteria in chickpea breeding programmay be useful for breeders to introduce suitable drought resistant chickpeacultivars for arid regions.

scholarly journals The fruit metric trait characterization of scarlet eggplant using the high-throughput tomato analyzer software

2021 ◽  
Vol 66 (2) ◽  
pp. 139-154
Author(s):  
Olawale Adeniji ◽  
Modinat Adekoya ◽  
Peter Jonah ◽  
Innocent Iseghohi ◽  
Charity Aremu
Keyword(s):  
Genotypic Variation ◽  
Fruit Size ◽  
Fruit Shape ◽  
Trait Variation ◽  
Yield Improvement ◽  
Fruit Size And Shape ◽  
Metric Traits ◽  
Shape And Size ◽  
Tomato Analyzer

Scarlet eggplant (Solanum aethiopicum [L.]) is an indigenous, underutilized fruit vegetable in Africa. Preference for fruit shape and size is high among growers and consumers. Fruit metric traits are important for yield improvement. Fruit metric descriptors are important contributors to variation, phenotypic and genotypic variation, and heritability. However, the measurement of these traits is cumbersome and subjective. Forty-three accessions were evaluated in 2016 and 2017. At maturity, 5 fruits were randomly harvested from each accession, digitalized and processed using the Tomato Analyzer software. Sixteen fruit metric traits were automatically generated and submitted for analysis of variance and multivariate analysis. The accessions differed over fruit size and shape due to genetic make-up. Fruit metric trait variation among S. aethiopicum groups was less influenced by the environment. The cv. Gilo group has oblong fruits, the cv. Shum group fruits are circular and ovoid; the cv. Kumba group fruits are less circular, lobed and flattened. AE/113 (C3), FUO 1 (C1) and FUO 5 (C2) Gilo groups are promising for fruit size. There were phenotypic plasticity and overlapping for fruit metric traits between the Gilo and Shum groups due to a common genome. The Tomato Analyzer software was able to discriminate accessions based on fruit phenomic traits, and the information could be used to establish commonalities between groups.

scholarly journals The effect of the blue light on the pericarp cell growth and lycopene content of cherry tomato (Solanum lycopersicum var. cerasiforme) fruit

2020 ◽  
Vol 4 (4) ◽  
pp. First
Author(s):  
Dien Thi Kieu Pham ◽  
Kiet Thuong Do ◽  
Sanh Du Nguyen
Keyword(s):  
Blue Light ◽  
Tomato Fruit ◽  
Fruit Size ◽  
Total Content ◽  
Fruit Growth ◽  
Cell Diameter ◽  
Cherry Tomato ◽  
Blue Filter ◽  
Lycopene Content

The cherry tomato fruit size depends on the growth of the pericarp which is parenchymal cells. The blue light stimulates the expansion of cotyledon cells, hypocotyl cells and leaf cells. In this study, the cherry tomato fruit was used as a material to investigate the effects of the blue light on the pericarp cells growth in fruit growth stage and lycopene accumulation in fruit growth and ripening stage. After 7 days of the blue light (440, 450 or 460 nm) treatment, pericarp cells growth and physiological, biochemical changes of the pericarp cells of 7-day-old fruit pericarp piece in vitro were analyzed. The lycopene content and some organic compound contents of 42-day-old postharvest fruits treated by the blue light similarly in 7 days and 7, 21-day-old fruit wrapped with blue filter (440-510 nm filtered) in 7 days were measured. The results showed that the 450 nm wavelength blue light the increased pericarp thickness of 7-day-old fruits through the increasement of the pericarp cell diameter. The 460 nm wavelength blue light the increased lycopene content of 42-day-old postharvest fruits. The blue filter treatment increased the sugar total content of 7- day-old fruits and increased the lycopene content of 21-day-old fruits.

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