scholarly journals ROLE OF SEED STORAGE RESERVES IN OSMOCONDITIONING OF SWEET CORN

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 662a-662
Author(s):  
G.B. McClure ◽  
N.S. Lang

Interconversions of seed storage reserves during osmoconditioning (controlled imbibition of water) may influence seed performance under suboptimal conditions. Sweet corn (Zen mays L. cv. Florida Staysweet) storage reserve changes were examined during osmoconditioning in relation to seed germination performance. Seeds were osmoconditioned in two experiments using distilled water (duration 3, 6, 9, 12, and 24 h) and polyethylene glycol 8000 solutions (0, .5, and 1.0 MPa for 12, 24, 48, 72 and 96 h). Germination performance was evaluated at 10 and 25C, and seed moisture, carbohydrate, and protein concentrations were quantified at each water potential x duration combination. Germination performance was not significantly improved by any treatment at 25C. Germination percentage at 10C was increased 10% for seeds osmoconditioned for 24 h in distilled water, and time to germination was decreased 50%. For seeds osmoconditioned 12 and 48 h at .5 and 1.0 MPa, respectively, germination percentage at 10C was increased 15%. Time to germination was reduced 50% for seeds osmoconditioned at .5 and 1.0 MPa after 48 and 96 h, respectively. Starch levels increased for seeds osmoconditioned at higher water potentials, but remained the same or decreased at lower water potentials.

2021 ◽  
Vol 22 (21) ◽  
pp. 12032
Author(s):  
Manpreet Kaur ◽  
Yamini Tak ◽  
Surekha Bhatia ◽  
Bavita Asthir ◽  
José M. Lorenzo ◽  
...  

Carbohydrates are the major storage reserves in seeds, and they are produced and accumulated in specific tissues during the growth and development of a plant. The storage products are hydrolyzed into a mobile form, and they are then translocated to the developing tissue following seed germination, thereby ensuring new plant formation and seedling vigor. The utilization of seed reserves is an important characteristic of seed quality. This review focuses on the seed storage reserve composition, source–sink relations and partitioning of the major transported carbohydrate form, i.e., sucrose, into different reserves through sucrolytic processes, biosynthetic pathways, interchanging levels during mobilization and crosstalk based on vital biochemical pathways that interlink the carbon and nitrogen cycles. Seed storage reserves are important due to their nutritional value; therefore, novel approaches to augmenting the targeted storage reserve are also discussed.


2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
A. Suma ◽  
Kalyani Sreenivasan ◽  
A. K. Singh ◽  
J. Radhamani

The role of relative humidity (RH) while processing and storing seeds ofBrassicaspp. andEruca sativawas investigated by creating different levels of relative humidity, namely, 75%, 50%, 32%, and 11% using different saturated salt solutions and 1% RH using concentrated sulphuric acid. The variability in seed storage behaviour of different species ofBrassicawas also evaluated. The samples were stored at40±2°Cin sealed containers and various physiological parameters were assessed at different intervals up to three months. The seed viability and seedling vigour parameters were considerably reduced in all accessions at high relative humidity irrespective of the species. Storage at intermediate relative humidities caused minimal decline in viability. All the accessions performed better at relative humidity level of 32% maintaining seed moisture content of 3%. On analyzing the variability in storage behaviour,B. rapaandB. junceawere better performers thanB. napusandEruca sativa.


HortScience ◽  
1992 ◽  
Vol 27 (9) ◽  
pp. 993-996 ◽  
Author(s):  
William J. Carpenter ◽  
Joseph F. Boucher

Light, temperature, relative humidity (RH), and GA3 affect vinca [Catharanthus roseus (L.) G. Don] seed storage and/or germination. GA3 failed to increase the germination percentage in darkness but significantly increased the percentage in continuous light. Similarly, GA3 treatment reduced both the number of days required to achieve 50% of the final germination percentage (T50) and the span between 10% and 90% germination (T90 — T10) for seeds in light, but not in darkness. Germination percentages were maximal and about equal at 25, 30, or 35C in darkness; germination was lowest below 25C. Germination T50 and T90 — T10 required the fewest days between 25 and 35C. Reducing seed moisture from 9.9% to 3.9% increased the T50 from 2.4 to 3.0 days but failed to change germination percentages. Germination percentage declined linearly as seed storage temperatures were reduced from 5 to — 20C, whereas days to T50 increased. Seed storage for 12 months without reduction in germination percentage was possible at 5C and 11%, 33%, or 52% RH, but storage at 75% or 95% RH for periods exceeding 1 month reduced germination. Seeds stored at 33% or 52% RH required fewer days to T50 than did seeds stored at 11%, 75%, or 95% RH. Chemical name used: gibberellic acid (GA3).


1981 ◽  
Vol 23 (2) ◽  
pp. 267-280 ◽  
Author(s):  
Minoru Murata ◽  
Eric E. Roos ◽  
Takumi Tsuchiya

In order to study the genetic changes which occur during seed storage, barley (Hordeum vulgare L. 'Himalaya') seeds were subjected to artificial aging using six combinations of temperature (21 °C, 32 °C, and 38 °C) and seed moisture content (12% and 18%). With increasing time in storage, germination of the seeds was delayed and reduced. Abnormal seedlings without roots also occurred with increased storage. Higher temperature and seed moisture content induced rapid loss of germinability. At the first mitotic division in the root tips, the frequency of aberrant anaphases and of roots with aberrations increased with increased storage time. The frequencies of aberrant anaphases and of roots with aberrations were also increased by higher temperature and seed moisture content. Frequencies of aberrant anaphases and of roots with aberrations were negatively correlated with germination percentage. This indicated that the frequency of chromosomal aberrations induced by seed aging might be estimated from the germination percentages.


2020 ◽  
Vol 9 (9) ◽  
pp. e858998157
Author(s):  
Érica Coutinho David ◽  
Bressa Karolina Dias Cardoso ◽  
Josiene Amanda dos Santos Viana ◽  
Eniel David Cruz

Knowledge about seed storage behavior is important to preserve plant species. Clitoria fairchildiana R.A.Howard is an endemic species with medicinal properties, it is used in the recovery of degraded land. The objective of this study is to evaluate the effect of drying on the physiological quality of C. fairchildiana seeds. Seeds were oven dried along with silica gel for 18 days and in intervals of 2 or 3 days, samples were removed to assess the seed moisture content. This was done in an oven at 105±3 oC for 24h, using four replicates with 10 seeds each. After undergoing the above-mentioned treatment, a sample of seeds was stored in the freezer (-18 ºC) for 3 months. Sowing was carried out in plastic trays in a sand and sawdust mixture (1:1), previously sterilized in hot water (100 oC) for two hours. Seeds were left to germinate in a laboratory with no temperature and relative humidity control. Germination tests were performed over 14 days with daily counts of the number of emerged seedlings. The percentage of seed germination, abnormal seedlings and dead seeds were obtained 14 days after sowing. The experimental design was completely randomized with four replications of 25 seeds. The reduction of seed moisture content from 18.5% to 5.6% affected physiological seed quality causing a reduction in the germination percentage and an increase in dead seeds and abnormal seedling percentage. C. fairchildiana seeds are classified as intermediate and they can be desiccated up to 8.6% with no reduction in physiology quality.


2015 ◽  
Vol 169 (1) ◽  
pp. 391-402 ◽  
Author(s):  
Mingxun Chen ◽  
Bin Zhang ◽  
Chengxiang Li ◽  
Harikrishna Kulaveerasingam ◽  
Fook Tim Chew ◽  
...  

2005 ◽  
Vol 33 (1) ◽  
pp. 280-282 ◽  
Author(s):  
L.D. Gómez ◽  
S. Baud ◽  
I.A. Graham

We previously showed that trehalose-6-phosphate synthase 1 (TPS1), which catalyses the first step in trehalose synthesis, is essential for embryo maturation in Arabidopsis [Eastmond, van Dijken, Spielman, Kerr, Tissier, Dickinson, Jones, Smeekens and Graham (2002) Plant J. 29, 225–235]. The tps1 mutant embryos develop more slowly than wild type. Patterning in the tps1 embryos appears normal but they do not progress past the torpedo stage to cotyledon stage, which is when storage reserves start to accumulate in the expanding cotyledons. Our initial data led to the hypothesis that trehalose metabolism plays a key role in regulating storage reserve accumulation by allowing the embryo to respond to the dramatic increase in sucrose levels that occurs at the torpedo stage of embryo development. More recent data demonstrate that while the tps1 mutant is blocked in the developmental progression of embryos from torpedo to cotyledon stage the expression of genes involved in the accumulation of storage reserves proceeds in a similar fashion to wild type. Thus it appears that induction of metabolic processes required for accumulation of storage reserves in tps1 occurs independently of the developmental stage and instead follows a temporal programme similar to wild-type seeds in the same silique.


2019 ◽  
Vol 7 (3) ◽  
pp. 277
Author(s):  
Ferawati Oktia Nurhayani ◽  
Arum Sekar Wulandari

The suitable seed storage method is important to maintain seed viability. This study aimed to examine the effect of periods and media storage on the viability of cananga seed (Cananga odorata (Lam.) Hook.f. & Thomson forma genuina). The treatments used in the seed viability test were 0, 2, 4, and 6 weeks as period storage and cocopeat, ash, and charcoal as media storage. The seeds were stored in the plastic at room temperature (± 25°C). The variables observed in this study were seed moisture content, percentage of germination, maximum growth potential, average days of germination, T50, germination value, and the number of seeds that germinate during storage. The percentage of C. odorata seeds germination without storage is 54%. The seed storage for 2 – 6 weeks in ash and charcoal hush caused their viability was drop. The seeds storage of C. odorata up to 6 weeks in cocopeat could maintain seeds viability with a percentage of germination that has no significant difference compared to control. In general, cocopeat can be used as one of the storage media in the seed storage of C. odorata before sowing.Keywords: Cananga odorata, cocopeat, germination percentage, seed, viability


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