scholarly journals 441 PB 244 CULTURE OF ISOLATED EMBRYOS OF DETERIORATED MAIZE SEED: A STRATEGY FOR RESCUING GERMPLASM

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 494d-494
Author(s):  
Sheila A. Blackman ◽  
Eric E. Roos
Keyword(s):  
Fungal Growth ◽  
Seed Storage ◽  
Naphthalene Acetic Acid ◽  
Growth Substances ◽  
Maize Seed ◽  
Radicle Growth ◽  
Coleoptile Growth ◽  
Sterile Culture ◽  
Maize Seeds

The low quality of some seed lots received by germplasm repositories such as the National Seed Storage Laboratory can thwart efforts to regenerate seed for storage. This germplasm is in danger of irretrievable loss. The aim of this work is to promote the germination, and hence regeneration, of such low quality seeds through sterile culture of the isolated embryos. Hybrid (B73×LH51) maize seeds were aged 5 y at 32°C and 0.037 g H2O g-1 dry wt. Vigor - but not viability -declined under these conditions. The effects of four factors on growth and germination were systematically examined. These were: seed pretreatments; antibiotics and fungicides; nutrients; and growth substances. Amongst the pretreatments, none surpassed partial hydration of seeds for 24 hr to 0.55 g H2O g-1 dry wt at 25°C prior to embryo dissection. Thiram (2.4 mg mL-1) and kanamycin (50 ug ml1) effectively controlled bacterial and fungal growth with no deleterious effects on growth during culture of the isolated embryos. Exogenous sucrose (optimum 5 % wt/vol) significantly stimulated radicle growth in both deteriorated and non-deteriorated embryos. No other organic or inorganic nutrient stimulated growth. Naphthalene acetic acid did not affect growth while kinetin reduced radicle growth and stimulated coleoptile growth. Gibberellic acid (GA3 at 10-5M) significantly stimulated radicle growth in deteriorated embryos, whereas it promoted coleoptile growth in both deteriorated and non-deteriorated embryos. These data suggest GA or a GA-stimulated process may limit the growth of aged embryos.

scholarly journals Optimization and sensitivity analysis of fast ethanol assay in maize seeds

2019 ◽  
Vol 41 (1) ◽  
pp. 97-107 ◽  
Author(s):  
Damrongvudhi Onwimol ◽  
Thunyapuk Rongsangchaicharean ◽  
Pitipong Thobunluepop ◽  
Tanapon Chaisan ◽  
Wanchai Chanprasert
Keyword(s):  
Quality Assessment ◽  
Ethanol Production ◽  
Seed Quality ◽  
Storage Conditions ◽  
Ambient Conditions ◽  
Maize Seed ◽  
Seed Deterioration ◽  
Maize Seeds ◽  
Hermetic Seal

Abstract: The evaluation of seed deterioration is very important to control the quality of the seeds stored. This study aimed to investigate the potential of fast ethanol assay for seed quality assessment of maize stored under different conditions. The first experiment was to determine the incubating temperature, incubating time, and amount of seed used in the assay. The results showed that the best protocol for the detection of headspace ethanol was incubation of 3 g of maize seed with 20% moisture content (wet basis) in a 20 mL gas chromatography vial at 70 °C for 1.5 h. The assay induced approximately 200-700 µg.L-1 of headspace ethanol, which was sufficient to identify seeds with different vigour levels. In the second experiment, the optimal conditions were used for quality assessment in aged maize seed stored for 12 months under different storage conditions. The increase in the ethanol production of stored maize seed under the controlled conditions (15 °C and 20% RH in the hermetic seal) was lower than under ambient conditions. The ethanol production levels of maize seed samples at the start of storage was significantly lesser than at six months storage (p < 0.05). The test limitations in deteriorated seed with different cultivars and ages will be discussed.

scholarly journals Effect of production environments on storage and physiological quality of maize seed

2021 ◽  
Vol 8 ◽  
pp. 1-15
Author(s):  
Itala Thaísa Padilha Dubal ◽  
Ivan Ricardo Carvalho ◽  
João Roberto Pimentel ◽  
Cristian Troyjack ◽  
Vinícius Jardel Szareski ◽  
...  
Keyword(s):  
Dry Matter ◽  
Seed Quality ◽  
Maize Seed ◽  
Large Format ◽  
Maize Seeds ◽  
Production Environments ◽  
Physiological Quality ◽  
Large Round ◽  
Physiological Characters

Storage is a factor that can affect seed quality as a function of latent damages. The losses that most contribute to the reduction in germination and vigor during storage are those caused by humidity, mechanical and temperature-related, that may interfere with seed quality throughout storage. The aim of this study was evaluating the physiological quality of maize seeds produced in two environments and classified in different formats compared to storage periods. The experiment was carried out in the 2016 harvest in two cultivation environments: Entre-Ijuís, RS and Pelotas, RS. Seeds were stratified by thickness through sieves with oblong holes and stratified into small round, large round, small flat and large flat. Growing environments influence the physiological characteristics of maize seeds. The flat and large format seeds have superior physiological quality for the measured physiological characters. The small and round seeds present lower values for germination, first germination count, emergence speed index, shoot dry matter of emergence seedlings and shoot dry matter of germination seedlings. Corn seeds when stored under controlled conditions remain for long periods without causing decreases in physiological quality.

scholarly journals Physical Properties of Maize Seed and its Effect on Sowing Quality and Variable Distance of Individual Plants

2018 ◽  
Vol 66 (1) ◽  
pp. 35-42
Author(s):  
Pavol Findura ◽  
Koloman Krištof ◽  
Ján Jobbágy ◽  
Peter Bajus ◽  
Urszula Malaga‑Toboła
Keyword(s):  
Maize Seed ◽  
Engineering Research ◽  
Average Value ◽  
Agricultural Engineering ◽  
Agronomic Crops ◽  
Maize Seeds ◽  
Variable Distance ◽  
Minimal Damage ◽  
Sowing Machine

Precision sowing has been a major thrust of agricultural engineering research for many years; however, most of the research and development work has dealt with seeders for agronomic crops. Sowing quality means required vertical (depth) and horizontal (spatial) spacing of seeds in the soil with minimal damage to the seeds. The spatial distribution of seeds (horizontal spacing) is given by distance between the rows and spacing of the seeds in a row. The objective of the study was the evaluation and comparison of the sowing quality of sowing machine JD MaxEmerge XP with mechanical filling of ladle holes with holding fingers within usage of seeds with different dimensional and shape attributes in agricultural conditions. As it was observed that shape attributes have a significant effect on seeding quality and at the end it affects emergency and whole further growth of maize seeds. Sowing machine JD MaxEmerge XP reached good results for the quality especially when using the angular seeds. It reached the sowing quality expressed by the standard deviation of 46.95–50.76 % when seeds PR 37 N01 and for seeds PR 37 N01 it is 47.43–48.73 %. Another evaluative criterion is dual sowing of seeds or plants (DRR), it was reached the average value 3.65–2.45 % for angular seeds and the average of 1.54–3.71 % for approximately spherical seeds.

scholarly journals PHYSIOLOGICAL AND SANITARY QUALITIES OF MAIZE LANDRACE SEEDS STORED UNDER TWO CONDITIONS

2015 ◽  
Vol 39 (4) ◽  
pp. 339-347 ◽  
Author(s):  
Raquel Stefanello ◽  
Marlove Fátima Brião Muniz ◽  
Ubirajara Russi Nunes ◽  
Cláudia Braga Dutra ◽  
Iana Somavilla
Keyword(s):  
Seed Quality ◽  
Accelerated Aging ◽  
Storage Period ◽  
Cold Test ◽  
Maize Seed ◽  
Maize Landrace ◽  
Maize Seeds ◽  
Plastic Bag ◽  
Physiological Quality

The preservation of seed quality during the storage period depends not only on the conditions during production and harvesting but also on the storage and maintenance of appropriate storage product conditions. Thus, the aim of this study was to evaluate the physiological and sanitary qualities of maize landrace seeds stored under two conditions. The maize seed batch varieties Oito carreiras, Cabo roxo and Lombo baio were used. Tests included germination, first count, cold test, accelerated aging and sanity. Based on the results it was concluded that the physiological quality of these seed varieties decreased with the storage period. The major fungi identified in the maize seeds during storage were from the genera Aspergillus, Fusarium and Penicillium, which caused deterioration and reduction of the physiological quality. Storage using a paper bag at a temperature of 10 °C did not prevent the deterioration of maize seeds but was more effective at preserving the quality of the seed compared with a plastic bag at room temperature.

scholarly journals The Influence of Rooting Substrate and Growth Regulators Indol butyric Acid and Naphthalene Acetic Acid in the Number and Length of Adventitious Roots to Hardwood Cuttings in Blueberry cv. ‘Bluecrop’ (Vaccinium corymbosum L.)

2016 ◽  
Vol 4 (4) ◽  
pp. 28
Author(s):  
Sabri Braha ◽  
Petrit Rama
Keyword(s):  
Growth Regulators ◽  
Growing Season ◽  
Vaccinium Corymbosum ◽  
Naphthalene Acetic Acid ◽  
Important Variation ◽  
Physiological Processes ◽  
One Year ◽  
The Impact ◽  
Better Than

The purpose of this research is to determine the impact of the turf-only substrate and turf–perlite in the ratio 2:1 and of growth regulators in the quality of adventive roots ( the number and length) of well lignified one-year old branches without fruit buds in the Bluecrop cultivar (Vaccinium corymbosum L.) taken at the end of the latent period before budding at the February 15 th during the -2015 growing season. In order to support the increase of the number of roots and their length the hardwood cuttings are treated with different IBA and NAA concentrations (1500, 3000, 4500 ppm), while a part of cuttings were untreated control. The number and the length of roots have increased in relation to the increase of concentration from 1500 to 3000 ppm followed by a decline of these values in concentrations over 3000 ppm. Respectively, the number of roots (8) and the higher values of root length (4.6 cm) are achieved in the turf–perlite substrate, IBA 3000 ppm (compared to the turf-only substrate). The presence of perlite helps the aeration of the substrate and supports biochemical and physiological processes which lead to the inducing of adventive roots. Regarding the number and length of roots an important variation for (p<0.05) was observed between different concentrations of IBA and NAA. In general the effect of IBA was a lot better than the effect of NAA.

scholarly journals ACCESSIBILITY OF APPLICATION OF GROWTH REGULATORS IN VITICULTURE

Russian vine ◽  
2020 ◽  
Vol 14 ◽  
pp. 37-43
Author(s):  
I.A. Avdeenkо ◽  
Keyword(s):  
Growth Regulators ◽  
Growth Substances ◽  
Promising Direction ◽  
The Past ◽  
Russian Federa Tion

Over the past few years in the Russian Federa-tion, especially in the Krasnodar Territory and Crimea, there has been an increase in the area of vine plantations. In this regard, carrying out additional operations that increase the rooting rate and quality of grape seedlings and cut-tings is a promising direction in nursery. The use of growth substances for rooting grape cuttings has proven to be the simplest and most economical way to achieve these goals. The paper analyzes the results of studies by various authors, proving the effectiveness of the use of growth substances on a grape plant.

scholarly journals Effects of nano copper used in seed treatment for germination, growth, and productivity of maize

2018 ◽  
Vol 40 (4) ◽  
Author(s):  
Le Thi Hien ◽  
Nguyen Tuong Van
Keyword(s):  
Metal Nanoparticles ◽  
Seed Treatment ◽  
Crude Protein ◽  
Zea Mays L ◽  
Crop Yields ◽  
Agricultural Products ◽  
Nutritional Content ◽  
Maize Seed ◽  
Treatment Technology

Recently, metal and other nanoparticles have been widely used to improve crop growth and development, reduce used chemical amount, increase safety of agricultural products and prevent soil and water pollution. Seed treatment technology with metal nanoparticles to stimulate seed germination and improve crop yields has been reported in several studies. In order to assess the safety of the use of metal nanoparticles in agriculture production, in this study the effect and safety of seed treatment with nanoparticles (nCu) prior to sowing on the germination, growth, development, productivity and quality of maize (Zea mays L.) were evaluated in the field conditions. Seeds of maize cultivar LVN 092 were treated with nCu at different concentrations before planting. Germination and plant growth rates, plants yield and grain nutrition were monitored and evaluated. The obtained results showed that treatment of maize seed with nCu at a concentration of 20 mg/kg seed increased germination after 7 days of sowing as well as the theoretical and actual yields compared to the control and the treatment of 1000 mg nCu/1 kg seeds. Other agricultural characteristics, such as the development rate, plant height, grain nutritional content including moisture, ash, total fiber, crude protein, minerals were not significantly between treatments and the control. Therefore, the seed treatments with metal nanoparticles can be contribute to improve agriculture production without impact to quality of crops. 

scholarly journals CNSL Concentration and Natural Dyes Effects in Formulation of Botanical Pesticide on Sitophilus zeamais and Maize Seed Quality

2020 ◽  
Vol 4 (1) ◽  
pp. 1-9
Author(s):  
Dian Astriani ◽  
Wafit Dinarto ◽  
Aji Jatmiko
Keyword(s):  
Seed Quality ◽  
Sitophilus Zeamais ◽  
Natural Dyes ◽  
Maize Weevil ◽  
Maize Seeds ◽  
Botanical Pesticide ◽  
Randomized Design ◽  
Efficiency And Effectiveness ◽  
Push Down

Maize is an important commodity in Indonesia and is vulnerable to maize weevil (Sitophilus zeamais) during storage which can cause a 100% yield loss. Chasew Nut Shell Liquid (CNSL) is a botanical pesticide able to control S. zeamais. Increasing CNSL efficiency and effectiveness requires additional adjuvants, such as solvents and dyes. Natural dyes addition is intended to provide seed markers. This study aimed to determine the best CNSL and natural dye formulation to control maize weevil and maintain maize seeds quality during storage. The research was conducted from August to October 2018 at the Agronomy Laboratory, Faculty of Agroindustry, Yogyakarta Mercu Buana University. The research was a single factor consisted of seven treatments with four replications arranged in a completely randomized design (CRD). Treatments were combinations of CNSL concentrations with the addition of natural dyes from suji leaf and teak leaves with one untreated control. The results of the research showed that CNSL as a seed treatment botanical pesticide could cause mortality and suppress S. zeamais populations and maintain the quality of maize seeds during storage for 12 weeks. Treatments with 20% CNSL formulation concentration and the addition of natural dyes both leaf pleomele or teak leaves could cause higher mortality and push down more populations and also could better maintain seed quality than 10 and 0% CNSL formulation concentrations.

scholarly journals Characteristics of Seed Storage Protein Affecting the Eating Quality of Japonica and Tongil-type Rice

2016 ◽  
Vol 61 (4) ◽  
pp. 227-234
Author(s):  
Jieun Kwak ◽  
◽  
Jeom-Sig Lee ◽  
Mi-Ra Yoon ◽  
Mi-Jung Kim ◽  
...  
Keyword(s):  
Storage Protein ◽  
Seed Storage Protein ◽  
Seed Storage ◽  
Eating Quality

scholarly journals Physiological quality of pink pepper seeds (Schinus terebinthifolia Raddi.) as a function of storage

2021 ◽  
Vol 38 (1) ◽  
pp. 144-157
Author(s):  
Thaísa De Oliveira Silveira ◽  
Madelon Rodrigues Braz ◽  
Gilmara Pires de Moura Palermo ◽  
Tiago Böer Breier
Keyword(s):  
Moisture Content ◽  
Storage Time ◽  
Storage Period ◽  
Seed Storage ◽  
Dry Weight ◽  
Native Forest ◽  
Alternative Source ◽  
Physiological Quality ◽  
And Storage

Seeds of native forest species for food and seedling production have a growing demand that impacts the need for studies involving seed storage in order to maintain good levels of germination quality. Thus, scientific analyzes that explore the physiological potential of the seeds of these species are necessary, especially for the pink pepper (Schinus terebinthifolia Raddi.), a species used as an alternative source of income for traditional communities and which has been arousing interest due to the consumption of its fruits as a condiment in the national and international market. This work aimed to assess the physiological quality of pink pepper seeds according to the storage period and seed size. The seeds came from a rural settlement, a pioneer in the extraction of pink pepper, in the state of Rio de Janeiro, Brazil. The seeds were evaluated for moisture content, germination, first count, length and dry weight of the germinated seedlings, during the period of time that they remained stored and the size of the seeds. The results allowed to conclude that: i) the germinative percentage of the stored pink pepper seeds decreased over the months; ii) the moisture content of the seeds decreased over the months evaluated iii) the different sizes of seeds did not show differences in germination and storage time and iv) values of length and dry weight of the seedlings did not differ according to the period of storage.

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