scholarly journals Ethylene Evolution is Positively Correlated with Seed Vigor in Sweet Corn and Tomato Seed Lots with Differing Vigor Levels but Similar Germination Capacity

2003 ◽  
Vol 128 (4) ◽  
pp. 608-614 ◽  
Author(s):  
Gunching Siriwitayawan ◽  
A. Bruce Downie ◽  
Robert L. Geneve
Keyword(s):  
Ethylene Production ◽  
Seed Quality ◽  
Sweet Corn ◽  
Accelerated Aging ◽  
Acc Oxidase ◽  
Seed Vigor ◽  
Germination Capacity ◽  
Ethylene Evolution ◽  
Age Related ◽  
Aged Seeds

Sweet corn (Zea mays L.) and tomato (Lycopersicon esculentum Mill.) seeds were aged naturally for 18 months or artificially aged using saturated salt accelerated aging to provide seed lots that differed in seed vigor, but retained a high standard germination percentage. Seed vigor was confirmed using standard vigor tests, including time to radicle emergence, cold, and accelerated aging tests. Ethylene evolution from both sweet corn and tomato seeds during germination was positively correlated with seed quality. Differences in ethylene evolution between nonaged and aged seeds were greater in seeds germinated on exogenous 1-aminocyclopropane-1-carboxylic acid (ACC). After 36 hours, there was about a 15-fold increase in ethylene evolution from seeds treated with 5 mm ACC compared to untreated seeds. Naturally and artificially aged seeds responded similarly and showed reduced ethylene production compared to nonaged seeds. In contrast to ethylene production, endogenous ACC titers were less for nonaged compared to aged seeds. Exogenous application of ACC to artificially aged seeds reduced the time to radicle protrusion, but did not completely reverse age-related effects on vigor. The data indicate that the reduced ability to produce ethylene in aged seeds was related to ACC oxidase (ACCO) synthesis or activity. Using Northern blot analysis, ACCO mRNA was detected after 48 hours of imbibition in nonaged seeds, but was undetectable in aged seeds affirming the contention that ACCO synthesis was delayed or reduced by aging. The current study provides additional support for ethylene as a biochemical indicator of seed vigor in seed lots with reduced vigor but high germination capacity.

scholarly journals Assessment of the physiological potential of super sweet corn seeds

2013 ◽  
Vol 35 (3) ◽  
pp. 340-346 ◽  
Author(s):  
Renata Oliveira Alvarenga ◽  
Julio Marcos-Filho ◽  
Tathiana Silva Timóteo
Keyword(s):  
Seed Quality ◽  
Sweet Corn ◽  
Seedling Emergence ◽  
Primary Root ◽  
Accelerated Aging ◽  
Cold Test ◽  
Seed Vigor ◽  
Corn Seed ◽  
Control Programs ◽  
Seedling Length

The assessment of physiological potential is essential in seed quality control programs. This study compared the sensitivity of different procedures for evaluating super sweet corn seed vigor, focusing on the primary root protrusion test. Six seed lots, each of the SWB 551 and SWB 585 hybrids, were used. Seed physiological potential was evaluated by germination and vigor tests (speed of germination, traditional and saturated salt accelerated aging, cold test, seedling length, seedling emergence and primary root protrusion). Primary root protrusion was evaluated every 12 hours at 15 °C, 20 °C and 25 °C using two criteria (primary root protrusion and seedlings at the 2 mm root stage). It was concluded that the primary root protrusion test at 15 °C can evaluate super sweet corn seed vigor by counting the number of seedlings at the 2 mm root stage.

scholarly journals 339 ACC-DERIVED ETHYLENE PRODUCTION, A RAPID TEST FOR SEED VIGOR

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 479b-479
Author(s):  
Anwar A. Khan
Keyword(s):  
Ethylene Production ◽  
Sweet Corn ◽  
Growth Parameters ◽  
Accelerated Aging ◽  
Natural Aging ◽  
Rapid Test ◽  
Storage Conditions ◽  
Seed Vigor ◽  
Snap Bean ◽  
Relationship Of

1-aminocyclopropane-1-carboxylic acid (ACC) was used to measure seed vigor of lettuce, cabbage, tomato, snap bean and sweet corn seeds. Accelerated aging at 40C and 93% RH over saturated solution of KH2PO4 and natural aging under ambient storage conditions (5-7C, 28 to 60% RH) were used to obtain seeds of differing vigor levels. Depending on the type of seeds, the amount of ACC needed for maximal ethylene production (saturating dose) ranged from 0.25 to 2mM. Seeds produced much larger amounts of ethylene in the presence than in absence of ACC, the ACC-derived ethylene was detected prior to germination, and ACC had no adverse effect on germination. ACC-derived ethylene production paralleled vigor loss as determined by percentage germination, mean germination time (for lettuce only) and seedling growth (for snap bean only). Second degree polynomial and logarithmic equations generated for the relationship of ACC-derived ethylene production to germination or growth parameters following seed aging, provided good to excellent fit. As a vigor test, the ACC-ethylene procedure has several advantages over the non-ACC ethylene procedure: it greatly improves the sensitivity of the test by enhancing ethylene production, it permits detection of small differences in vigor levels and it allows detection of ethylene prior to germination within a few hours of soaking.

scholarly journals Pola Penurunan Viabilitas dan Pengembangan Metode Pendugaan Vigor Daya Simpan Benih Pepaya (Carica Papaya L.)

2017 ◽  
Vol 7 (3) ◽  
pp. 146
Author(s):  
Astryani Rosyad ◽  
M. Rahmad Suhartanto ◽  
Abdul Qadir
Keyword(s):  
Moisture Content ◽  
Aging Time ◽  
Seed Quality ◽  
Initial Moisture Content ◽  
Accelerated Aging ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Vigor ◽  
Develop Model ◽  
Papaya Seed

<p>ABSTRACT<br />Information of seed quality during storage can be determined through the actual storage and storability vigor estimation. This study aimed at comparing effective accelerated aging method<br />between physical and chemical, and studying the seed deterioration during storage in ambient (T =28-30 0C, RH=75-78%) and AC (T =18-20 0C, RH =51-60%) condition with three levels of initial moisture content (8-10%, 10-12%, and 12-14%) for 20 weeks. The final objective of this research<br />was to develop model for storability vigor of papaya seed. Two experiments, accelerated aging and seed storage were conducted at Seed Laboratory, Department of Agronomy and Horticulture, Bogor Agricultural University from October 2015 to May 2016. A completely randomized design with nested factors and four replications was applied to both experiments. The results showed that physical accelerated aging using IPB 77-1 MMM machine was more effective than chemical accelerated aging using IPB 77-1 MM machine for papaya seed. The viability of seed stored in AC condition remained high until the end of the storage period, whereas it declined at 16 week storage period in the ambient condition. The viability of seed with initial moisture content of 12-14% declined faster than that of initial moisture content of 8-10% after 18 week storage periode. The model used to estimate the storability vigor of papaya seed accurately was the equation y = a + b expcx where y : storability vigor estimation, x : aging time and a,b,c : constant value. Simulation of storability vigor estimation with constant value of a, b, c and input of aging time can estimate storability seed vigor in actual storage.<br />Keywords: accelerated aging, IPB 77-1 MM machine, IPB 77-1 MMM machine, seed storage, simulation</p><p>ABSTRAK<br />Informasi mutu benih selama penyimpanan dapat diketahui melalui penyimpanan secara aktual dan pendugaan vigor daya simpan. Penelitian ini bertujuan untuk membandingkan metode<br />pengusangan cepat yang efektif antara fisik dengan kimia serta mempelajari pola penurunan viabilitas benih selama penyimpanan aktual pada kondisi simpan kamar (suhu =28-30 0C, RH =75-78%) dan AC (suhu =18-20 0C, RH =51-60%) dengan tiga tingkat kadar air awal (8-10%, 10-12%, dan 12-14%) selama 20 minggu. Tujuan akhirnya adalah membangun model vigor daya simpan benih pepaya. Penelitian pengusangan cepat dan penyimpanan dilakukan pada bulan Oktober 2015 sampai Mei 2016 di Laboratorium Benih, Departemen Agronomi dan Hortikultura, Institut Pertanian<br />Bogor. Kedua penelitian menggunakan rancangan acak lengkap tersarang dengan empat ulangan. Hasil penelitian menunjukkan bahwa pengusangan cepat secara fisik dengan alat IPB 77-1 MMM lebih efektif daripada pengusangan kimia dengan alat IPB 77-1 MM untuk benih pepaya. Viabilitas benih yang disimpan pada kondisi AC tetap tinggi hingga akhir periode simpan, sedangkan pada kondisi kamar penurunan viabilitas dimulai pada periode simpan 16 minggu. Benih yang disimpan dengan tingkat KA awal sebesar 12-14% lebih cepat mengalami penurunan viabilitas mulai periode simpan 18 minggu dibandingkan dengan benih dengan KA awal 8-10%. Hasil penelitian juga menunjukkan terdapat korelasi yang erat antara pola kemunduran benih pada pengusangan cepat dan penyimpanan aktual, sehingga model pendugaan vigor daya simpan (y) berdasarkan waktu pengusangan (x) dapat disusun dengan persamaan y = a + b expcx. Simulasi pendugaan vigor daya simpan dengan nilai konstanta a, b, dan c serta input waktu pengusangan dapat menduga vigor daya simpan benih selama penyimpanan aktual.<br />Kata kunci: alat IPB 77-1 MM, alat IPB 77-1 MMM, pengusangan cepat, penyimpanan benih,<br />simulasi</p>

scholarly journals Digital images of seedling for evaluating coffee seed vigor

2019 ◽  
Vol 41 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Heiber Andres Trujillo ◽  
Francisco Guilhien Gomes-Junior ◽  
Silvio Moure Cicero
Keyword(s):  
Seed Quality ◽  
Digital Images ◽  
Imaging System ◽  
Primary Root ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Control Programs ◽  
Scanned Images ◽  
Coffea Arabica L ◽  
Coffee Seed

Abstract: The digital image analysis of seedlings has become largely employed in seed quality-control programs due to its feasibility, objectivity and fast results. Despite these advantages, no studies have yet demonstrated the efficiency of this technique for analyzing coffee seed vigor. The present study aimed at evaluating the efficiency of the Seed Vigor Imaging System (SVIS®) in determining the vigor of coffee seeds (Coffea arabica L.) from digital images of seedlings. Also, the results of these analyses were contrasted with conventional vigor tests. Six seed lots from each cultivar, Bourbon and Catucaí 20/15, were used. The research was conducted in two experimental times, and the seed vigor was determined by the tests of first germination count, accelerated aging, electrical conductivity, primary root protrusion speed, as well as by the analysis of scanned images of seedlings, made possible by the SVIS® software. For the Bourbon and Catucaí 20/15 cultivars, the SVIS® analysis was able to discriminate lots of both high and low vigor (vigor indexes of 317 to 752, and 181 to 703, respectively). A similar outcome was obtained from the conventional tests appraised in this research. Eventually, it was possible to conclude that the digital analysis of 20-day-old seedlings by SVIS® is an efficient method to evaluate coffee seed vigor.

scholarly journals 573 Seed Vigor Testing for Small-seeded Flower Species using Computer-aided Image Analysis

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 545C-545
Author(s):  
Kay Oakley ◽  
Robert Geneve ◽  
Sharon Kester ◽  
Patchara Wonprasaid
Keyword(s):  
Seed Quality ◽  
Digital Images ◽  
Accelerated Aging ◽  
Petri Dish ◽  
Seed Vigor ◽  
Radicle Growth ◽  
Agronomic Crops ◽  
Computer Aided ◽  
Initial Seed ◽  
Radicle Length

Standardized seed vigor tests must be developed for greenhouse-grown flower species. Current vigor tests used to evaluate large-seeded agronomic crops are generally not useful for evaluating smaller-seeded flower species. One alternative is to use radicle length in seedlings grown under controlled environments as an indicator of seed vigor. For that purpose, a seed vigor test was developed that uses digital images taken using a flat bed scanner to measure radicle length in small-seeded flower species. A novel, cellulose substrate was used for germinating seeds. It provided similar moisture-holding properties to standard germination blotters used by commercial seed analysts, but is clear. This has allowed for quick image acquisition without removing seedlings from the petri dish. Correlations were made between seedling growth (radicle length, total seedling length, and total seedling area) with other vigor tests (saturated salts accelerated aging) and greenhouse plug flat emergence. For several seed lots of impatiens that varied in initial seed quality, radicle length after 4 days showed good correlations (>R2 = 0.79) with other measures of seed vigor for describing seed quality. This system is an improvement over other attempts to use computer-aided assessment of digital images because it provides digital images that do not vary due to external lighting; it uses software that can evaluate radicle length in a petri dish assay that does not require a slant-board for straight radicle growth; it relies on standard germination technics used by every seed lab; it uses a clear substrate to replace the opaque blotter to allow digital images to be taken within the petri dish; and accurate measurements of seedling parts is performed in under 2 min per petri dish.

scholarly journals Harvest delay, storage and physiological quality of soybean seeds

2019 ◽  
Vol 41 (4) ◽  
pp. 506-513
Author(s):  
Rafael Vergara ◽  
Raimunda Nonata Oliveira da Silva ◽  
Arieli Paula Nadal ◽  
Gizele Ingrid Gadotti ◽  
Tiago Zanatta Aumonde ◽  
...  
Keyword(s):  
Seed Quality ◽  
Local Development ◽  
Block Design ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Storage Period ◽  
Seed Vigor ◽  
Soybean Seeds ◽  
Randomized Block Design ◽  
Harvest Delay

Abstract: Soybean is one of the leading commodities in Brazilian agribusiness. Its cultivation is widespread in different seed-producing regions of the country, where it generates income and local development. In this context, the soybean seed is a fundamental input, as its quality strongly influences the success of the crop. However, the period after the achievement of physiological maturity is critical for the maintenance of seed quality. For that reason, this study aimed at evaluating the effect of harvest delay on both the initial and final qualities of soybean seeds. The research consisted of four harvest times, in which the initial quality was assessed through tests of first germination count, germination, accelerated aging and tetrazolium. After 120 days of storage, the germination and accelerated aging tests were once again conducted. The experiment complied with a completely randomized block design with eight replications. The rainfall was monitored during the pre-harvest phase. All variables experienced negative impacts due to the delay in harvesting. Also, the seeds suffered more damage as the delay progressed, and the variables germination and seed vigor decreased after the storage period.

scholarly journals Muskmelon seed priming in relation to seed vigor

2004 ◽  
Vol 61 (1) ◽  
pp. 114-117 ◽  
Author(s):  
Warley Marcos Nascimento ◽  
Fernando Antônio Souza de Aragão
Keyword(s):  
Low Temperature ◽  
Interaction Effect ◽  
Cucumis Melo ◽  
Seed Quality ◽  
Germination Rate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Seed Vigor ◽  
Cucumis Melo L ◽  
Aged Seeds

A number of important factors may affect seed priming response, including seed quality. Effects of seed vigor on seed priming response were investigated using seed lots of two muskmelon (Cucumis melo L.) cultivars. Seeds of muskmelon, cvs. Mission and Top Net SR were artificially aged at 43°C for 0, 20 and 40 hours. Seeds were primed for six days in darkness at 25°C in KNO3 (0.35 mol L-1) aerated solution. Aged seeds germinated poorly at 17°C. Priming increased germination rate at 17 and 25°C and germination percentage at 17°C. An interaction effect on germination performance between vigor and priming was observed, especially at low temperature. Priming increased germination performance in seeds of low vigor, and the response was cultivar dependent.

scholarly journals Improved assessment of wheat seeds vigor

2012 ◽  
Vol 36 (6) ◽  
pp. 608-614 ◽  
Author(s):  
Tereza Cristina de Carvalho ◽  
Francisco Carlos Krzyzanowski ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco
Keyword(s):  
Moisture Content ◽  
Filter Paper ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Wheat Seed ◽  
Efficient Procedure ◽  
Tetrazolium Test ◽  
Wheat Seeds

The tetrazolium test is widely used for seed vigor assessment of various plant species. For wheat, however, there is no methodology specifically recommended. This study aimed at determining an efficient procedure to evaluate vigor of wheat seeds by this test; besides allowing the establishment of seed quality classes. Thus, four different wheat seed lots were assessed by determining the moisture content and through tests of germination, accelerated aging, and field seedling emergence. The following methodologies were assessed: 1) longitudinal bisection of seed, with subsequent placement of the two halves on filter paper moistened with a 1.0% tetrazolium solution, at 30º C, for 2 h; 2) longitudinal bisection, with subsequent immersion of one seed half into a 1.0% tetrazolium solution, at 30º C, for 3 h; and 3) longitudinal bisection, with subsequent immersion of one seed half into a 0.075% tetrazolium solution, at 40º C, for 2 h. It was concluded that the tetrazolium test is more efficient in evaluating wheat seed vigor when performed with immersion of one half of the seed into a 0.075% tetrazolium solution (40º C, for 2 h) or a 0.1% tetrazolium solution (30º C, during 3 h); allowing to sort seeds into four quality classes.

Influence of Harvest and Post-Harvest Conditions on the Physiology and Germination of Peanut Kernels

1999 ◽  
Vol 26 (2) ◽  
pp. 64-68 ◽  
Author(s):  
G. Dey ◽  
R. K. Mukherjee ◽  
S. Bal
Keyword(s):  
Seed Quality ◽  
Membrane Integrity ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Peanut Kernel ◽  
Physical Damage ◽  
Post Harvest ◽  
Drying Procedures ◽  
Aging Conditions ◽  
Storage Effects

Abstract Harvesting, storage, and shelling procedures can greatly affect seed vigor. In regions where mechanical harvesting and drying procedures are not standard, seeds are often damaged and germination is poor. The objective of this research was to study the effects of different drying procedures on seed quality. Germination of peanut kernel (Arachis hypogaea L., cv. J-11) was significantly affected by harvesting and post-harvest handling and operations. Harvesting at or around 110 d after. emergence showed higher numbers of matured kernels, with high quality and better storability. Pods after harvest were dried in the field following different methods. Field drying of pods with haulms, in heaps to avoid direct exposure to sunlight following the DOR method, was found to be highly suitable in reducing mechanical injury during shelling and also in maintaining higher germinability of seeds in storage. Effects of shelling were assessed in terms of physical damage. Only sound mature kernels (SMK) were used for drying and storage tests, which involved temperatures ranging from 20–50 C (in an air velocity 0.61 to 1.0 m/sec) and ambient to 50% RH conditions. Effects of drying on occurrence of physical damage like % split kernel, bold kernel, and skin slip then were assessed. Both shelling and drying had shown significant effects on germinability, seedling vigor, membrane integrity, dehydrogenase activity, and lipid peroxidation when tested under ambient or accelerated aging conditions. Slow drying or low temperature/low humidity drying reduced physical damage and maintained high vigor and viability during storage.

scholarly journals Saturated salt accelerated aging and computerized analysis of seedling images to evaluate soybean seed performance

2014 ◽  
Vol 36 (2) ◽  
pp. 213-221 ◽  
Author(s):  
Juliana Tieme Yagushi ◽  
Denis Santiago Costa ◽  
José de Barros França-Neto
Keyword(s):  
Seed Quality ◽  
Imaging System ◽  
Seedling Emergence ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Seed Vigor ◽  
Accelerated Aging Test ◽  
Aging Test ◽  
Physiological Quality ◽  
Tetrazolium Test

Seed deterioration causes losses to seed companies during the storage. For researches, the challenge has been to develop techniques which improve the seed longevity as well as laboratory analysis methods required for evaluating the evolution of deterioration in storage. This study had the objective of determining the effectiveness of the accelerated aging test with a saturated NaCl solution and Seed Vigor Imaging System (SVIS) analysis in checking soybean seed physiological in comparison with the recommended vigor tests. Soybean seeds from the cultivars BRS 184 and M-Soy 7908 RR were used, each of them were represented by six lots and stored for six months under two environment conditions. Seed physiological quality was checked every two months in storage through germination evaluations, accelerated aging (different methods), tetrazolium test (vigor and viability) seedling emergence, and SVIS analysis. The tetrazolium test is the most advisable method for evaluating soybean seed quality during storage. The results of the saturated salt accelerated aging test with NaCl at 41 ºC for 72 or 96 hours and the SVIS evaluation are also both efficient for estimating the physiological potential of seeds.

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