scholarly journals FEATURES OF STOMATOLOGIC STATUS, TELOMERE LENGTHS AND PHENOTYPE OF IMMUNOCOMPETENT CELLS OF PERIPHERAL BLOOD IN CHILDREN – RESIDENTS OF UKRAINIAN TERRITORIES, CONTAMINATED WITH RADIONUCLIDES

2017 ◽  
Vol 5 ◽  
pp. 59-68 ◽  
Author(s):  
Svetlana Liubarets ◽  
Irina Iliyenko ◽  
Tetiana Liubarets ◽  
Olena Lyaskivska ◽  
Nataliia Bidenko ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Peripheral Blood ◽  
Molecular Genetic ◽  
Relative Length ◽  
Immunocompetent Cells ◽  
Permanent Teeth ◽  
Control Group ◽  
Enamel Hypoplasia ◽  
Degree Relative ◽  
Flow Cytofluorometry

Objective of research. To analyze features of stomatological status indexes, relative length of telomeres and phenotype of immunocompetent cells of peripheral blood in children with chronological enamel hypoplasia – residents of Ukrainian territories, contaminated with radionuclides after the Chornobyl NPP accident. Materials and methods. The object of research is chronological enamel hypoplasia (CEH) of permanent teeth, and lymphocytes of peripheral blood (PB). The subject of research is the state of oral cavity hygiene, caries intensity, gingivitis severity degree, relative telomere length (RTL), and phenotype of immunocompetent cells (ICC) of peripheral blood (PB). Methods of the research: stomatological, immunological (flow cytofluorometry), molecular genetic (fluorescence in situ hybridization-flow cytometry – flow-FISH), statistical. Results. Two types of the analysis of the stomatologic status, RTL and phenotype of PB ICC were carried out: the first is the comparison of indexes of the combined group of children with anamnesis complicated by radiation (group No. 1 with CEH + group No. 2 without CEH) comparing with the control group No. 3; the second is the analysis of indeces of the group No. 1 comparing with the same markers in children of the groups No. 2 and No. 3. The first stage of the analysis shows thesignificant worsening of oral hygien indices , high DMFTand DMFS in children with permanent dentition, higher degree of gums inflamation and moresignificant RTL shortening in children of the combined group (No. 1 + No. 2) comparing with the control. Changes of the same character, but more expressed observed during the another analysis concerning the children with anamnesis complicated by radiation and CEH comparing with the control. Reliable changes in the content of PB ICC subpopulations in the examined children of all groups were not identified. Conclusions. 1. Inchildren with CEH, complicated by ionizing radiation (IR) influence in low doses diapason the highest degree of stomatological status disorders comparing with the examined reference and control groups was determined: unsatisfactory and poor oral cavity hygiene according to OHI-S (1.76±0.41, р˂0.0001 and р˂0.0001, respectively) and Silness-Loe index (1.73±0.45, р˂0.0001 and р˂0.0001, respectively); very high intensity of dental caries ((DMFT=7.0±2.29, р=0.003and р˂0.0001; DMFS=8.74±2.58, р=0.0004 and р˂0.0001, respectively); mean degree of gingivitis severity (РМА 47.55±14.27 %, р˂0.0001 and р˂0.0001, respectively). 2. At CEH, which occurs due to the complex of negative factors during first years of life, including IR in residents of territories with effective doses more than 1.0 mSv/year, reliable shortening of relative telomeres length of PB lymphocytes comparing with the control group (15.17±3.20, р=0.0002) was found. Reliable differences in the content of peripheral blood immunocompetent cells when compared the examined groups were not identified. 3. Results of indices of the groups No. 1, No. 2 and No. 3 comparison are agreed with the data, obtained in result of dispersive analysis that allowed to separate the most informative indices concerning stomatological examination of children: OHI-S index (F=34.92; р˂0.001); Silness-Loe index (F=27.01; р˂0.001); dft+DMFT (F=82.23; р˂0.001); dfs+DMFS (F=49.41; р˂0.001); DMFT (F=13.94; р˂0.001); DMFS(F=19.41; р˂0.001); РМА (F=41.25; р˂0.001); RTL (F=7.53; р=0.001).

scholarly journals Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol

2013 ◽  
Vol 82 (3) ◽  
pp. 337-342
Author(s):  
Viera Revajová ◽  
Zuzana Slaminková ◽  
Ľubomíra Grešáková ◽  
Mikuláš Levkut
Keyword(s):  
Flow Cytometry ◽  
Peripheral Blood ◽  
Broiler Chickens ◽  
White Blood Cells ◽  
Intraepithelial Lymphocytes ◽  
Immunocompetent Cells ◽  
Control Group ◽  
Low Doses ◽  
Mhc Ii ◽  
And Performance

Morphometry and flow cytometry for intraepithelial lymphocyte phenotyping were used to determine the changes in duodenal mucosae after administration of low doses of deoxynivalenol in chickens. Moreover, functions of phagocytes and immunocompetent cells in peripheral blood were evaluated by flow cytometry. In total, sixty chickens of Ross hybrid broilers 308 were used in this experiment. Two experimental groups of 20 birds were continually fed for 14 days a diet containing deoxynivalenol at a dose of 1 and 3 mg·kg-1; 20 birds of the control group were fed uncontaminated diet. Morphometry showed only tendency to decrease the height of villi and surface area of duodenal mucosae in chickens fed the diet supplemented with 3 mg·kg-1 deoxynivalenol. Phenotyping of intraepithelial lymphocytes showed a decrease of CD45+ (P < 0.034) in duodenum of birds fed diets supplemented with deoxynivalenol. Examination of white blood cells showed a decrease of monocytes (P < 0.020) in chickens fed 3 mg·kg-1 of deoxynivalenol. Both experimental groups revealed higher metabolic burst of peripheral blood heterophils (P < 0.001). Phenotyping of immunocompetent cells showed an increase (P < 0.003) of CD3+ and a decrease (P < 0.001) of MHC II+ cells in peripheral blood of chickens fed with 3 mg·kg-1 dose of deoxynivalenol. The experimental feeding of chickens with deoxynivalenol resulted in immunomodulation of immunocompetent cells in duodenum and blood with mild atrophy of intestinal villi, mainly after the feeding of the dose of 3 mg·kg-1. We proved that even low doses of deoxynivalenol can cause changes in haemathological, immunological and morphological profiles already during two weeks, and lead to the activation of compensatory-adaptive mechanisms with unfavourable impact on health and performance of birds.

scholarly journals DENTAL CARIES INTENSITY IN CHILDREN WITH DISTURBANCES IN TOOTH FORMATION, SURVIVED AFTER THE CHORNOBYL NPP ACCIDENT

2021 ◽  
Vol 26 ◽  
pp. 426-436
Author(s):  
S. Liubarets ◽  
Keyword(s):  
Dental Caries ◽  
Ionizing Radiation ◽  
High Intensity ◽  
Permanent Teeth ◽  
Dental Occlusion ◽  
Control Group ◽  
Molar Incisor Hypomineralisation ◽  
Enamel Hypoplasia ◽  
Tooth Formation ◽  
Permanent Occlusion

Objective: assessment of dental caries intensity in children with disturbances in tooth formation living in radiologically contaminated territories as a result of the Chornobyl NPP accident. Materials and methods. Children aged 6-14 years old (n = 1470) suffering from disturbances in tooth formation (DTF) with the residents of radiologically contaminated territories (n = 528) among them were enrolled in the study. The DTF subtypes were assayed, namely the systemic enamel hypoplasia (SEH) and molar incisor hypomineralisation (MIH). Personal radiation history was recorded. Intensity of caries and caries of surfaces in the mixed (i.e. transitional) occlusion (df+DMF, dfs+DMFS coefficients) and permanent occlusion (DMF, DMFS coefficients) was identified. Results. Caries intensity in the exposed children suffering DTF with mixed and permanent dental occlusion was significantly higher vs. either in children exposed to ionizing radiation with no DTF or in the control group (р < 0.001, р < 0.001 and р < 0.05, р < 0.001 correspondingly). The highest caries intensity of the permanent teeth was revealed in the residents of contaminated territories suffering SEH (6.95 ± 2.3) vs. cases of MIH (5.68 ± 1.97) as a result of nonsymmetrical teeth involvement. Conclusions. The highest values of df+DMF, dfs+DMFS coefficients were diagnosed in children with a mixed dental occlusion aged 6-14 years old living on territories contaminated as a result of the ChNPP accident as compared to the not exposed subjects and control group (7.2 ± 2.07 and 8.98 ± 2.4, р < 0.001). A very high intensity of caries and surface caries of permanent teeth (DMF = 6.79 ± 2.34, DMFS = 8.69 ± 2.75, р < 0.001) was established in children exposed to ionizing radiation and suffering DTF. Such a high intensity may be due to the impact of a set of negative factors including the ionizing radiation in low doses and peculiarities of economic component of social status of study subjects, namely the unbalanced diet. In case of permanent occlusion the intensity of caries and caries of tooth of in children survived after the ChNPP accident and suffering SEH was significantly (р < 0.05) higher compared to the persons suffering MIH. Key words: children, caries, disturbances in tooth formation, systemic enamel hypoplasia, molar-incisor hypomineralization, ionizing radiation, ChNPP accident.

scholarly journals Monitoring of cytogenetic instability by micronuclei assay of both immunocompetent and non-immunocompetent cells in tick-borne encephalitis patients depending on variants of glutathione-S-transferase genes in the genotype

2019 ◽  
Vol 9 (3-4) ◽  
pp. 600-606
Author(s):  
Nikolay Nikolaevich Ilyinskikh ◽  
Ekaterina Nikolaevna Ilyinskikh ◽  
Evgenia Vladimirovna Zamyatina ◽  
Svetoslava Vyacheslavovna Lee
Keyword(s):  
T Lymphocytes ◽  
Peripheral Blood ◽  
Micronucleus Assay ◽  
Immunocompetent Cells ◽  
Control Group ◽  
Buccal Cells ◽  
Glutathione S Transferase ◽  
Tick Borne Encephalitis ◽  
Buccal Mucous Membrane ◽  
The One

Aim of this study was to study the dynamics of the frequency of cytokinesis-blocked T-lymphocytes with micronuclei in peripheral blood and the frequency of buccal micronucleated epithelium cells for a period of half a year in patients with acute tick-borne encephalitis, depending on burden of active and inactive variants of glutathione-S-transferase genes (GSTM1 and GSTT1) in the patient's genotype. We carried out micronucleus assay in immunocompetent and non-immunocompetent cells in 54 patients with acute tick-borne encephalitis and 35 healthy persons (control) residing in the Tomsk and Tyumen regions. To analyze the frequency of cytokinesis-blocked micronucleated T-lymphocytes was used venous peripheral blood as material for phytohemagglutinin-stimulated cultures, and to study the frequency of buccal micronucleated cells, samples of the buccal mucous membrane epithelial cells were obtained. To carried out both techniques of micronucleus assay, cytological preparations were prepared, which were stained using the Giemsa or Felgen methods. The material for the study was obtained repeatedly during admission of patients to treatment, and also after 1 week, 1, 3 and 6 months.  Polymerase chain reaction was used to analyze the alleles of the GSTM1 and GSTT1 genes. As a result of this analysis was found a significant increase in the frequency of micronucleated cells in tick-borne encephalitis patients compared with the control group. In addition, the frequency of cytokinesis-blocked micronucleated T-lymphocytes was increased significantly higher than the one of micronucleated buccal cells. The most significant and prolonged increase in the frequency of micronucleated cells was associated with the mutant inactive variants of the genes GSTM1 (0/0) and GSTT1 (0/0). In the patients with burden the inactive forms of these genes, the cytogenetic instability of the cytokinesis-blocked blood T-lymphocytes could persist for up to six months. In case of buccal cells, the frequency of micronucleated cells was close to the one in the control group as early as 1-3 months after a course of treatment. Conclusion. It was found that the most increased and prolonged frequency of cytogenetically instable cells persisted in cytokinesis-blocked T-lymphocytes of peripheral blood of patients with tick-borne encephalitis who were carriers of the genotype with inactive variants of  both GSTM1 (0/0) and GSTT1 (0/0 ) glutathione-S-transferase genes.

scholarly journals PREVENTION OF DENTAL CARIES IN THE CONDITIONS OF ECOLOGICAL POLLUTION

2018 ◽  
pp. 55-59
Author(s):  
I.V. Kovach ◽  
H.V. Shtompel ◽  
Ye.N. Dychko ◽  
A.V. Verbytska
Keyword(s):  
Dental Caries ◽  
Oral Cavity ◽  
Dental Health ◽  
Harmful Effect ◽  
Living Environment ◽  
Preschool Age ◽  
Permanent Teeth ◽  
Control Group ◽  
Basic Group ◽  
Dental Tissues

Modern domestic preventive dentistry has success in the theory of the origin and development of major dental diseases, practice of treatment, including dental caries, and uses these results to prevent children’s diseases in the country. However, the desired effectiveness of preventive measures have not been achieved primarily among the population and individual level, taking into account some specific characteristics of residence region, etiology and pathogenesis of the demineralization phenomena of hard dental tissues in different periods of child’s development. Adverse environmental conditions and technogenic character are not taken into account, when air, soil and groundwater are oversaturated with environmental contaminants and toxicants. They cause fatal breakage in biochemical reactions and metabolic processes in childhood and at a young age, when defective dental health brings a lot of problems in the adult period of life, and, as a result, reduces the life. Investigation of dental health had been conducted in 251 preschool age children (first age group consisted of 3-4 years-old, II group consisted of 5-6 – years-old with 123 and 128 people respectively). In addition, all the observed children were divided into two groups depending on the use of prophylactic anti-caries interventions: the basic group was in compliance with the "Program- maximum" and control the "Program- minimum". Approximately 60 members were in the monitoring teams of different age groups. The "Program-minimum" included measures of general health, balanced diet with the significant restriction of carbohydrate and perform twice a day hygiene actions in the oral cavity. The “Program – maximum” also included the ingestion of calcium citrate and amino acids with SH-group of methionine. Such treatment and prophylactic complex has the adaptive, antioxidant, antidote and immune resolving action. It makes sense for neutralization of eco toxicants – heavy metals in the area of children’s residence. It was found that level of Fe and Zn was in the threatening degree of content, and Cd is moderate in the living environment of the examined children. It provokes and enhances the glycolytic processes in the oral cavity. Harmful effect of toxicants in the oral cavity, and also in a children's organism as a whole, was clearly fixed in the mineralization of the skeleton flat bones of children. It was revealed with densitometry of the calcaneus that 53 individuals had  indicators, particularly BUA. They indicated the weakening of the skeleton mineralization. It indirectly pointed to problems in the hard tissues of the teeth. However, the use of treatment and prophylactic complex improved the bone structures density. The level of calcium and phosphate in the oral fluid of the observed individuals had recovered to optimal after three years of use of treatment and prophylactic complex, contributed to effective secondary mineralization both in temporary and in permanent teeth. Thus, the use of treatment and prophylactic complex in people of the basic group allowed quite convincingly and positively to impact on the incidence of caries of deciduous and permanent teeth. The rate of growth of dental caries intensity after three years of preventive interventions in the basic group was three times less than in the control group. At the same time, the caries preventive efficacy was 59-62%, and this is a very positive indicator. These results are evidence of proven necessity of prophylactic of dental health correction in children years of those people who reside in areas with a very complex technogenic environment due to metallurgical plants located in the Prydniprovsk region.

scholarly journals CHARACTERISTICS OF CYCLIN D1-MEDIATED REGULATION OF CELL CYCLE OF PERIPHERAL BLOOD LIMPHOCYTES OF CHORNOBYL CLEAN-UP WORKERS AND PERSONS WITH MALIGNANT NEOPLASMS OF THE ORAL CAVITY, OROPHARYNX AND LARYNGOPHARYNX

2021 ◽  
Vol 26 ◽  
pp. 357-370
Author(s):  
N. Golyarnik ◽  
◽  
I. Ilienko ◽  
L. Zvarych ◽  
M. Vorobyov ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Radiation Dose ◽  
Oral Cavity ◽  
Cyclin D1 ◽  
Peripheral Blood ◽  
Control Group ◽  
Malignant Neoplasms ◽  
Proliferative Potential ◽  
Regulation Of Cell Cycle ◽  
Oncological Pathology

Objective: to explore proliferative potential of peripheral blood lymphocytes of Chornobyl clean-up workers and persons with malignant neoplasms of the oral cavity, oropharynx and laryngopharynx by level of expression of cyclin D1 and quantitative parameters of cell cycle. Materials and methods. A total of 294 men aged (58.47 ± 7.32) were surveyed, 215 of them were Chornobyl clean-up workers (1986–1987), exposed at the dose range 10.43–3623.31 mSv; 49 persons of the control group and 30 persons with malignant neoplasms of the oral cavity, oropharynx and laryngopharynx at III, IVА and IVВ stages of the disease. The analysis of parameters of cell cycle and proliferative activity of peripheral blood (PB) lymphocytes was performed using the flow cytometry. The evaluation of distribution of cells by G0/G1, S, G2/M cell cycle phases was done in vivo and in in vitro. Proliferative potential was analyzed by level of expression of cytoplasmic protein of cyclin D1. Results. Proliferative potential of PB lymphocytes of Chornobyl clean-up workers and persons with malignant neoplasms of the oral cavity, oropharynx and laryngopharynx was assessed. An increase in the level of spontaneous сyclin D1 expression and disturbance of сyclin D1-dependent regulation of cell cycle of PB lymphocytes after mitogen activation were determined in the Chornobyl clean-up workers. An increase in pool of cells in the S- and G2/M-phases of cell cycle was detected, which characterizes high proliferative potential of PB lymphocytes. These changes are most pronounced in the subgroup of persons with a radiation dose of D < 500 mSv, and in persons with oncological pathology. Conclusions. A positive linear dependence has been established between the radiation dose and the number of cells in the S-phase of cell cycle in the subgroup of Chornobyl clean-up workers with a radiation dose of D < 500 mSv. The detected changes of cyclin D1-dependent regulation of cell cycle and proliferative status of lymphocytes depend on the radiation dose, can be a manifestation of genome instability and be a cause for risks of oncogenesis, in a remote period after radiation exposure. Key words: cell cycle, cyclin D1, oncological pathology, radiation, Chornobyl.

scholarly journals OPTIMIZATION OF PROPHYLACTIC ARRANGEMENTS IN CHILDREN WITH HYPOPLASIA OF DENTAL ENAMEL

2016 ◽  
Vol 4 ◽  
pp. 43-49
Author(s):  
Svetlana Liubarets ◽  
Nataliia Bidenko
Keyword(s):  
Oral Cavity ◽  
Dental Enamel ◽  
Permanent Teeth ◽  
Enamel Hypoplasia ◽  
Calcium Orthophosphates ◽  
Oral Liquid ◽  
Mineral Components ◽  
Group 2 ◽  
State Registration ◽  
Group 1

Aim of research. To raise the effectiveness of anticarious properties of oral cavity in children with enamel hypoplasia by introduction of the complex of arrangements, directed on optimization of the oral cavity hygiene, level of secretory immunoglobulin А (sIgA) and the content of mineral components of oral liquid (calcium and orthophosphates). Materials and methods. Object of research – enamel hypoplasia of the permanent teeth. Subject of research – hygienic status of the oral cavity, level of general calcium, orthophosphates, sIgA in the oral liquid. Methods of research: vital coloration of enamel, transillumination, photometric, immune-enzyme method, indices of hygienic status of the oral cavity (Green–Vermillion (1964) index, Silness–Loe (1964, 1967) index). There was carried out an assessment of hygienic status of the oral cavity, determination of the level of calcium, orthophosphates and sIgA in the oral liquid of 45 children, 6–14 years old (group № 1 – 21 persons, group № 2 – 24 persons). Group №1 – children, who underwent the complex of prophylactic arrangements that included recommendations as to the choice of means and subjects for the oral cavity care and teaching the standard method of teeth brushing. Group № 2 – children, who additionally used remineralizing cream on the water base with casein phosphopeptide – amorphous calcium phosphate (СРР-АСР) (Certificate on the state registration №10014/2010 of 03.12.2010). Results. Hygienic status of the oral cavity of children from the both groups was assessed as “unsatisfactory” one according to Green–Vermillion index and as “bad” one according to Silness–Loe index. After prophylactic arrangements the oral cavity status was improved in children of the group № 1: Green–Vermillion (p=0,0013) and Silness–Loe (p=0,0002) indices essentially decreased. In children of the group №2 after prophylactic arrangements hygienic status of the oral cavity was improved more essentially: Green–Vermillion (p=0,0002) and Silness–Loe (p=0,0002) indices reliably decreased. Prescription of remineralizing cream to the children of group № 2 reliably raised the content of calcium (р=0,0051) and sIgA in the oral liquid (р=0,001). Orthophosphates level did not essentially change in both groups. Conclusions. Introduction of the complex of prophylactic arrangements with additional use of remineralizing preparation favored the more essential improvement of the oral cavity hygiene, raise of calcium and sIgA in the oral liquid.

Oral and Oropharyngeal Sensory Function in Adults With Chronic Obstructive Pulmonary Disease

2020 ◽  
Vol 29 (2) ◽  
pp. 864-872
Author(s):  
Fernanda Borowsky da Rosa ◽  
Adriane Schmidt Pasqualoto ◽  
Catriona M. Steele ◽  
Renata Mancopes
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Oral Cavity ◽  
Pulmonary Disease ◽  
Thermal Sensation ◽  
Sensory Function ◽  
Control Group ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease ◽  
Age Related ◽  
Copd Patients

Introduction The oral cavity and pharynx have a rich sensory system composed of specialized receptors. The integrity of oropharyngeal sensation is thought to be fundamental for safe and efficient swallowing. Chronic obstructive pulmonary disease (COPD) patients are at risk for oropharyngeal sensory impairment due to frequent use of inhaled medications and comorbidities including gastroesophageal reflux disease. Objective This study aimed to describe and compare oral and oropharyngeal sensory function measured using noninstrumental clinical methods in adults with COPD and healthy controls. Method Participants included 27 adults (18 men, nine women) with a diagnosis of COPD and a mean age of 66.56 years ( SD = 8.68). The control group comprised 11 healthy adults (five men, six women) with a mean age of 60.09 years ( SD = 11.57). Spirometry measures confirmed reduced functional expiratory volumes (% predicted) in the COPD patients compared to the control participants. All participants completed a case history interview and underwent clinical evaluation of oral and oropharyngeal sensation by a speech-language pathologist. The sensory evaluation explored the detection of tactile and temperature stimuli delivered by cotton swab to six locations in the oral cavity and two in the oropharynx as well as identification of the taste of stimuli administered in 5-ml boluses to the mouth. Analyses explored the frequencies of accurate responses regarding stimulus location, temperature and taste between groups, and between age groups (“≤ 65 years” and “> 65 years”) within the COPD cohort. Results We found significantly higher frequencies of reported use of inhaled medications ( p < .001) and xerostomia ( p = .003) in the COPD cohort. Oral cavity thermal sensation ( p = .009) was reduced in the COPD participants, and a significant age-related decline in gustatory sensation was found in the COPD group ( p = .018). Conclusion This study found that most of the measures of oral and oropharyngeal sensation remained intact in the COPD group. Oral thermal sensation was impaired in individuals with COPD, and reduced gustatory sensation was observed in the older COPD participants. Possible links between these results and the use of inhaled medication by individuals with COPD are discussed.

Expression and Significance of Th17 Cells and Related Factors in Patients with Autoimmune Hepatitis

2019 ◽  
Vol 22 (4) ◽  
pp. 232-237 ◽  
Author(s):  
Jihong An
Keyword(s):  
Autoimmune Hepatitis ◽  
Peripheral Blood ◽  
Th17 Cells ◽  
Treg Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Serum Total Bilirubin ◽  
Study Group ◽  
Related Factors ◽  
Tnf Α

Objective: This study aims to investigate the expression and clinical significance of Th17 cells and related factors in peripheral blood of patients with Autoimmune Hepatitis (AIH). Methods: A retrospective selection of 100 patients with AIH were included as a study group, and 100 healthy volunteers in the outpatient clinic were selected as the control group. The levels of IL- 17, IL-6, IL-21 and TNF-α in peripheral blood of all subjects were detected by enzyme-linked immunosorbent assay and the frequency of Th17 cells and Treg cells was detected by flow cytometry. Results: Results showed that the study group had higher levels of serum total bilirubin (TBil), alkaline phosphatase (ALP), γ -glutamyltranspeptidase (γ-GT), immunoglobulin G (IgG), immunoglobulin M (IgM), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) than the control group, as well as higher levels of IL-17, IL-6, IL-21 and TNF-α in serum. The frequency of Th17 cells in peripheral blood was higher in the study group, while the frequency of Treg cells was lower. Also, serum IL-17, TNF-α levels and Th17 cells frequency were positively correlated with ALT and AST, whereas Treg cells frequency were negatively correlated with ALT and AST levels. Conclusion: Our finding demonstrates that Th17 cell frequency and their related factors IL-17 and TNF-α, are associated with liver damage, which might be used to monitor AIH disease severity.

scholarly journals Comparative evaluation of osteogenic differentiation potential of stem cells derived from dental pulp and exfoliated deciduous teeth cultured over granular hydroxyapatite based scaffold

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Manal Nabil Hagar ◽  
Farinawati Yazid ◽  
Nur Atmaliya Luchman ◽  
Shahrul Hisham Zainal Ariffin ◽  
Rohaya Megat Abdul Wahab
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Dental Pulp ◽  
3D Culture ◽  
Permanent Teeth ◽  
Deciduous Teeth ◽  
Osteogenic Potential ◽  
Control Group ◽  
Rt Pcr ◽  
Differentiation Potential

Abstract Background Mesenchymal stem cells isolated from the dental pulp of primary and permanent teeth can be differentiated into different cell types including osteoblasts. This study was conducted to compare the morphology and osteogenic potential of stem cells from exfoliated deciduous teeth (SHED) and dental pulp stem cells (DPSC) in granular hydroxyapatite scaffold (gHA). Preosteoblast cells (MC3T3-E1) were used as a control group. Methodology The expression of stemness markers for DPSC and SHED was evaluated using reverse transcriptase-polymerase chain reaction (RT-PCR). Alkaline phosphatase assay was used to compare the osteoblastic differentiation of these cells (2D culture). Then, cells were seeded on the scaffold and incubated for 21 days. Morphology assessment using field emission scanning electron microscopy (FESEM) was done while osteogenic differentiation was detected using ALP assay (3D culture). Results The morphology of cells was mononucleated, fibroblast-like shaped cells with extended cytoplasmic projection. In RT-PCR study, DPSC and SHED expressed GAPDH, CD73, CD105, and CD146 while negatively expressed CD11b, CD34 and CD45. FESEM results showed that by day 21, dental stem cells have a round like morphology which is the morphology of osteoblast as compared to day 7. The osteogenic potential using ALP assay was significantly increased (p < 0.01) in SHED as compared to DPSC and MC3T3-E1 in 2D and 3D cultures. Conclusion gHA scaffold is an optimal scaffold as it induced osteogenesis in vitro. Besides, SHED had the highest osteogenic potential making them a preferred candidate for tissue engineering in comparison with DPSC.

scholarly journals Autologous Peripheral Blood Mononuclear Cells for Limb Salvage in Diabetic Foot Patients with No-Option Critical Limb Ischemia

2021 ◽  
Vol 10 (10) ◽  
pp. 2213
Author(s):  
Alessia Scatena ◽  
Pasquale Petruzzi ◽  
Filippo Maioli ◽  
Francesca Lucaroni ◽  
Cristina Ambrosone ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Critical Limb Ischemia ◽  
Diabetic Foot ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Limb Ischemia ◽  
Control Group ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Peripheral blood mononuclear cells (PBMNCs) are reported to prevent major amputation and healing in no-option critical limb ischemia (NO-CLI). The aim of this study is to evaluate PBMNC treatment in comparison to standard treatment in NO-CLI patients with diabetic foot ulcers (DFUs). The study included 76 NO-CLI patients admitted to our centers because of CLI with DFUs. All patients were treated with the same standard care (control group), but 38 patients were also treated with autologous PBMNC implants. Major amputations, overall mortality, and number of healed patients were evaluated as the primary endpoint. Only 4 out 38 amputations (10.5%) were observed in the PBMNC group, while 15 out of 38 amputations (39.5%) were recorded in the control group (p = 0.0037). The Kaplan–Meier curves and the log-rank test results showed a significantly lower amputation rate in the PBMNCs group vs. the control group (p = 0.000). At two years follow-up, nearly 80% of the PBMNCs group was still alive vs. only 20% of the control group (p = 0.000). In the PBMNC group, 33 patients healed (86.6%) while only one patient healed in the control group (p = 0.000). PBMNCs showed a positive clinical outcome at two years follow-up in patients with DFUs and NO-CLI, significantly reducing the amputation rate and improving survival and wound healing. According to our study results, intramuscular and peri-lesional injection of autologous PBMNCs could prevent amputations in NO-CLI diabetic patients.

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