STUDY OF ANTI-INFLAMMATORY EFFECT OF CONCENTRATED JUICE AND GRANULES FROM VACCINIUM OXYCOCCOS FRUITS ON THE MODEL OF BACTERIAL CYSTITIS IN RATS

The fruits of marsh cranberries due to the content of phenolic compounds, in particular proanthocyanidins, are effective in the prevention and treatment of cystitis [1]. The aim of the research was to study the anti-inflammatory properties of condensed juice of Vaccinium oxycoccos fruits and granules “Cranberry” in a model of acute bacterial cystitis in rats. Materials and methods. The anti-inflammatory properties of condensed juice from Vaccinium oxycoccos fruits and granules “Cranberry” were studied in a model of acute bacterial cystitis in rats, which was caused by three daily transurethral injection into the bladder of 0.3 ml of E. coli strain at a concentration of 106 CFU/ml. “Canefron® H” was used as a reference drug. Tissues were studied histologically by assessing the structure of the bladder wall and the condition of blood vessels. Results. Infection of animals with E. coli resulted in a significant increase in the desquamation of the transitional epithelium, edematous changes and narrowing of blood vessels in the tissues of the bladder wall. During treatment with the drug “Canefron® N” there was a decrease in cell infiltration and desquamation of cells, dilation of blood vessels. When animals used condensed juice from Vaccinium oxycoccos fruits and granules “Cranberry” in the histological picture of the walls of the bladder there was a decrease in desquamation, edema and cellular infiltration, the lumen of blood vessels was approaching normal. Conclusions. The use of condensed juice from Vaccinium oxycoccos fruits and granules “Cranberry” was accompanied by a positive effect on the tissues of the walls and blood vessels of the bladder of rats (there was a decrease in desquamation of the superficial layers of the transitional epithelium and normalization of the histological picture of vascular entanglement, that was not inferior to the comparison drug “Canefron® N”.

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Therapeutic use of lowmolecular weight heparin for capillaritis alba

Phlebologie ◽

10.1055/s-0037-1622240 ◽

2008 ◽

Vol 37 (05) ◽

pp. 259-265 ◽

Cited By ~ 11

Author(s):

H. Kutzner ◽

G. Hesse

Keyword(s):

Case Studies ◽

Randomized Study ◽

Compression Therapy ◽

Venous Hypertension ◽

Therapeutic Use ◽

Prospective Randomized Study ◽

Anti Inflammatory ◽

Positive Effect ◽

Inflammatory Effect

SummaryThe reason of the so called ulcerated capillaritis alba or idiopathic atrophie blanche is vasculopathy caused by severe venous hypertension. Thrombosed and rarificated vessels worsen the oxygenation, increase permanent inflammation and impede the necessary compression therapy. The anti-inflammatory effects of heparin alleviate pain and being independent from the antithrombotic ones it needs much lower doses for treatment. This anti-inflammatory effect is now becoming more important in clinical phlebology. Case studies of more than 50 patients and one prospective randomized study of 87 patients clearly demonstrate the ameliorated healing of ulcerated atrophie blanche. In our office we could document this positive effect with 22 patients. We present the pathophysiology of low molecular heparins for ulcerated capillaritis alba and our own experiences with it.

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The Anti-Inflammatory Effect of a γ-Lactone Isolated from Ostrich Oil of Struthio camelus (Ratite) and Its Formulated Nano-Emulsion in Formalin-Induced Paw Edema

Molecules ◽

10.3390/molecules26123701 ◽

2021 ◽

Vol 26 (12) ◽

pp. 3701

Author(s):

Salah E. M. Eltom ◽

Ahmed A. H. Abdellatif ◽

Hamzah Maswadeh ◽

Mohsen S. Al-Omar ◽

Atef A. Abdel-Hafez ◽

...

Keyword(s):

Oral Administration ◽

Standard Procedure ◽

P Value ◽

Skin Thickness ◽

Paw Edema ◽

Reference Drug ◽

Struthio Camelus ◽

Anti Inflammatory ◽

Nano Emulsion ◽

Inflammatory Effect

The ostrich oil of Struthio camelus (Ratite) found uses in folk medicine as an anti-inflammatory in eczema and contact dermatitis. The anti-inflammatory effect of a γ-lactone (5-hexyl-3H-furan-2-one) isolated from ostrich oil and its formulated nano-emulsion in formalin-induced paw edema was investigated in this study. Ostrich oil was saponified using a standard procedure; the aqueous residue was fractionated, purified, and characterized as γ-lactone (5-hexyl-3H-furan-2-one) through the interpretation of IR, NMR, and MS analyses. The γ-lactone was formulated as nano-emulsion using methylcellulose (MC) for oral solubilized form. The γ-lactone methylcellulose nanoparticles (γ-lactone-MC-NPs) were characterized for their size, shape, and encapsulation efficiency with a uniform size of 300 nm and 59.9% drug content. The γ-lactone was applied topically, while the formulated nanoparticles (NPs) were administered orally to rats. A non-steroidal anti-inflammatory drug (diclofenac gel) was used as a reference drug for topical use and ibuprofen suspension for oral administration. Edema was measured using the plethysmograph method. Both γ-lactone and γ-lactone-MC-NPs showed reduction of formalin-induced paw edema in rats and proved to be better than the reference drugs; diclofenac gel and ibuprofen emulsion. Histological examination of the skin tissue revealed increased skin thickness with subepidermal edema and mixed inflammatory cellular infiltration, which were significantly reduced by the γ-lactone compared to the positive control (p-value = 0.00013). Diuretic and toxicity studies of oral γ-lactone-MC-NPs were performed. No diuretic activity was observed. However, lethargy, drowsiness, and refusal to feeding observed may limit its oral administration.

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Propionate catabolism by CD-associated adherent-invasive E. coli counteracts its anti-inflammatory effect

Gut Microbes ◽

10.1080/19490976.2020.1839318 ◽

2021 ◽

Vol 13 (1) ◽

pp. 1-18

Author(s):

Allison Agus ◽

Damien Richard ◽

Tiphanie Faïs ◽

Emilie Vazeille ◽

Mélissa Chervy ◽

...

Keyword(s):

E Coli ◽

Anti Inflammatory ◽

Inflammatory Effect

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Synthesis, Characterization and Acute Anti-inflammatory Evaluation of New Mefenamic Acid Derivatives Having 4-Thiazolidinone Nucleus

Iraqi Journal of Pharmaceutical Sciences ( P-ISSN 1683 - 3597 E-ISSN 2521 - 3512) ◽

10.31351/vol28iss1pp138-146 ◽

2019 ◽

Vol 28 (1) ◽

pp. 138-146

Author(s):

Mustafa H. Ali Alsafi ◽

Muthanna S. Farhan

Keyword(s):

Side Effects ◽

Mefenamic Acid ◽

Paw Edema ◽

Compound I ◽

Reference Drug ◽

Cox 2 ◽

Anti Inflammatory ◽

Derivatives Of ◽

Cox 1 ◽

Inflammatory Effect

Mefenamic acid (MA) is one of the non-steroidal anti-inflammatory drugs, it is widely used probably due to having both anti-inflammatory and analgesic activity, the main side effects of mefenamic acid include gastrointestinal tract (GIT) disturbance mainly diarrhea, peptic ulceration, and gastric bleeding. The analgesic effects of NSAIDs are probably linked to COX-2 inhibition, while COX-1 inhibition is the major cause of this classic adverse effects. Introduction of thiazolidinone may lead to the increase in the bulkiness leads to the preferential inhibition of COX-2 rather than COX-1 enzyme. The study aimed to synthesize derivatives of mefenamic acid with more potency and to decrease the drug's potential side effects, new series of 4-thiazolidinone derivatives of mefenamic acid were synthesized IVa-g. The synthetic procedures for target compounds and their intermediates are designed to be as follows: acylation of secondary amine of mefenamic acid by chloroacetylchloride to produce compound (I), then reaction between compound (I) and hydrazine hydrate to form hydrazine derivative of mefenamic acid (compound II). After that, Schiff base formation by addition of seven benzaldehyde derivatives and finally, cyclization in presence of thioglycolic acid to form 4-thiazolidinone heterocyclic ring. The characterization of the titled compounds has been established on the basis of their spectral FTIR, 1HNMR data, and by measurements of their physical properties. In vivo acute anti-inflammatory effect of the synthesized compounds was evaluated in rats using egg-white induced edema model of inflammation. The tested compounds and the reference drug produced significant reduction of paw edema with respect to the effect of dimethyl sulfoxide 10%v/v (control group). Compound IVe showed more potent effect than mefenamic acid at 240-300 min, while at time 300 min, compounds IVa and IVd exhibit more potent anti-inflammatory effect than mefenamic acid (50mg/kg, i.p.) as they reduced paw edema significantly more than mefenamic acid at mentioned intervals (p<0.05) . On the other hand compound IVc exhibited lower anti-inflammatory effect.

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Study to Investigate the anti-inflammatory effect of Codelac® Broncho with Thymus Serpyllum (elixir) in comparison with reference drug Fenspiride (syrup) using accute Carrageenan-induced Paw Inflammation Model

Research Results in Pharmacology ◽

10.3897/rrpharmacology.5.33233 ◽

2019 ◽

Vol 5 (1) ◽

pp. 45-52

Author(s):

Pavel D. Kolesnichenko ◽

Anna A. Peresypkina ◽

Artem A. Poromov ◽

Elena N. Kareva ◽

Alexey N. Demidenko

Keyword(s):

Inflammatory Activity ◽

C Reactive Protein ◽

Reference Drug ◽

Reactive Protein ◽

Model Control ◽

Anti Inflammatory ◽

Thymus Serpyllum ◽

Carrageenan Inflammation ◽

Subplantar Injection ◽

Inflammatory Effect

Introduction: Evaluation of anti-inflammatory action of Codelac® Broncho with Thymus Serpyllum (elixir) in comparison with Fenspiride was carried out on the model of acute carrageenan inflammation of the paws in rats. Materials and methods: Edema was caused by subplantar injection of 0.1 ml of 1% λ- carrageenan gel into the hind paw. The severity of edema was assessed by using 37140 plethysmometer (UGO BASILE, Italy). The measurements were performed before edema induction and 1, 2, 4, 12, 24, 48, 72, 96 and 120 hours afterwards. Anti-inflammatory activity of the drugs was also evaluated based on the analysis of rats’ blood, C-reactive protein concentration and histological examination results. Results and discussion: A decrease in the paw volume increment was revealed in the group with the studied drug in comparison with the group with the carrageenan edema model (control) 4, 12, 24 hours after injection of carrageenan (p<0.05). As a result of plethysmometry, a more pronounced anti-inflammatory effect of the studied drug than that of Fenspiride was revealed. There was a significant decrease in the levels of leukocytes (p<0.05), lymphocytes (p<0.05), monocytes (p<0.05) and neutrophils (p<0.05) in the group with the studied drug compared to those the the control 48 hours after the initiation of edema, while in the group with Fenspiride, there was only a decrease in the levels of leukocytes (p<0.05) and lymphocytes (p<0.05). There were no differences in the concentration of C-reactive protein between the groups. Conclusion: The obtained data indicate a more pronounced anti-inflammatory activity of Codelac® Broncho with Thymus Serpyllum in comparison with Fenspiride, on the model of acute carrageenan inflammation of the paw in rats.

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Antiinflammatory activity of 2-, 3-, 4-carboxymethylpyridinium hexafluorosilicates on carrageenan model of inflammation

Farmatsevtychnyi zhurnal ◽

10.32352/0367-3057.4.19.09 ◽

2019 ◽

pp. 82-87

Author(s):

B. V. Pristupa ◽

I. O. Shyshkin ◽

Ya. V. Rozhkovsky ◽

V. O. Gelmboldt

Keyword(s):

Antiinflammatory Activity ◽

Biologically Active ◽

Inflammatory Activity ◽

Male Rats ◽

Control Group ◽

Paw Edema ◽

Commercial Preparation ◽

Reference Drug ◽

Anti Inflammatory ◽

Inflammatory Effect

In the last decade, high cariesprophylactic efficacy of ammonium hexafluorosilicates, including salts with substituted biologically active ammonium cations, has been demonstrated. Among the promising anti-caries agents are recently synthesized 2-, 3-, 4-carboxymethylpyridinium hexafluorosilicates, for whose cations anti-inflammatory activity is expected. The aim of the work is to assess the presence of anti-inflammatory activity in the series of 2-, 3-, 4-carboxymethylpyridinium hexafluorosilicates in the experiment. 2-, 3-, 4-Carboxymethylpyridinium hexafluorosilicates (I–III, respectively) were synthesized according to the previously proposed technique, carrageenan is a commercial preparation, reference drug is a indomethacin. Experiments on the anti-inflammatory activity of hexafluorosilicates were performed on 77 white Wistar male rats weighing 174–190 g using the carrageenan model of inflammation. The inflammatory reaction was reproduced by subplantary administration of 0.1 ml of a 0.2% solution of carrageenan, the studied compounds were administered orally in doses 1/10, 1/20, 1/50 from LD50 for the compound III. It was established that after administration of carrageenan, animals of the control group showed marked paw edema, which gradually increased and was maximal after 24 hours of observation. According to the data obtained, the indices for compounds I-III practically do not differ from those of the control group. This indicates that, despite the results of the PASS forecast and the presence of acetic acid residue, an anti-inflammatory pharmacofor, in compounds I-III, the compounds under study do not have an anti-inflammatory effect in the carrageenan model. 2-, 3-, 4-Carboxymethylpyridinium hexafluorosilicates do not show the expected anti-inflammatory activity in the carrageenan model of inflammation.

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Biochemical Confirmation of the Anti-Inflammatory Effect of Coxibs and their Compositions with N-Acetylgulcosaminil 1-4-N-Acetylmuramoil-L-alanyl-D- Isoglagamin

Letters in Applied NanoBioScience ◽

10.33263/lianbs101.19441949 ◽

2020 ◽

Vol 10 (1) ◽

pp. 1944-1949

Keyword(s):

Blood Serum ◽

Biochemical Study ◽

Negative Control ◽

Inflammation Marker ◽

Reference Drug ◽

Anti Inflammatory ◽

Almost All ◽

Pharmaceutical Composition ◽

Better Than ◽

Inflammatory Effect

The current work is focused on the biochemical confirmation of the anti-inflammatory effect of coxibs and their compositions with licopid as an adjuvant for NSAIDs in the treatment of inflammation. The current investigation aimed to compare the anti-inflammatory effect of rofecoxib, celecoxib, licopid, and pharmaceutical compositions consisting of rofecoxib and licopid, celecoxib, and licopid. Sodium diclofenac was chosen as the reference drug. The anti-inflammatory effect of the test substances was studied using an experimental model of formalin-induced paw edema. The level of SA was determined using SialoTest (SPC Eco-Service). A biochemical study of the anti-inflammatory effect of rofecoxib, celecoxib, licopid, and their composition on the content of inflammation marker (SA) showed that almost all studied drugs reduced the content of SA in rat’s blood serum compared to the negative control. The results of the biochemical study showed that rofecoxib has a pronounced anti-inflammatory effect. It acts almost 1.2 times better than celecoxib, and the leader in our biochemical studies is a two-component pharmaceutical composition of celecoxib + licopid at the level of SA in blood serum under formalin-induced edema.

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ANTI-INFLAMMATORY EFFECT OF ZINGIBER OFFICINALE ON SPRAGUE DAWLEY RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i3.16251 ◽

2017 ◽

Vol 10 (3) ◽

pp. 353 ◽

Cited By ~ 1

Author(s):

Rohini Karunakaran ◽

Ndanusa Abdullahi Hassan ◽

Uma Sankar A ◽

Khin Mar Aye

Keyword(s):

Aqueous Extract ◽

Diclofenac Sodium ◽

Zingiber Officinale ◽

Control Group ◽

Sprague Dawley ◽

Reference Drug ◽

Sd Rats ◽

Sprague Dawley Rats ◽

Anti Inflammatory ◽

Inflammatory Effect

ABSTRACTObjective: To investigate the anti-inflammatory effect of aqueous extract of Zingiber officinale on carrageenan-induced inflammation on SpragueDawley (SD) rats.Methods: SD rats were divided into six of five groups and allowed to acclimatize for 1 week. Inflammation was induced on the animal by injecting theright hand paw with carrageenan (0.1 ml of 1%). Group 1 was given normal saline and served as a control. Group 2 was fed with food and water andserved as the carrageenan control. Group 3 was given 200 mg/kg aqueous extract of ginger, Group 4 with 400 mg/kg aqueous extract of ginger, andGroup 5 with 150 mg/kg diclofenac sodium (reference drug for inflammation).Results: The paw edema in carrageenan-induced SD rats was considerably reduced by treating with 400 mg/kg aqueous ginger extracts whencompared to the untreated SD rats (p<0.001).Conclusion: This study indicates that aqueous extract of Z. officinale possesses anti-inflammatory properties.Keywords: Anti-inflammatory, Sprague Dawley rats, Zingiber officinale, Carrageenan, Edema

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FORMULATION AND EVALUATION OF TOPICAL ANTI-INFLAMMATORY HERBAL GEL

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i7.33859 ◽

2019 ◽

pp. 252-255

Author(s):

MAHENDRA A GIRI ◽

RASIKA D BHALKE

Keyword(s):

Methanolic Extract ◽

Skin Irritation ◽

Storage Conditions ◽

Reference Drug ◽

Edema Volume ◽

Rat Paw Edema ◽

Ich Guidelines ◽

Anti Inflammatory ◽

Rat Paw ◽

Inflammatory Effect

Objective: The present investigation aims at development and evaluation of herbal anti-inflammatory gel using of methanolic extract from Emblica officinalis fruits (MEEOF) and Aegle marmelos fruits (MEAMF). Methods: The gels were prepared using Carbopol 934, various concentrations of MEEOF, MEAMF, propylene glycol 400, methylparaben, propylparaben, and required amount of distilled water. Then, skin pH (6.8-7) was maintained by dropwise addition of triethanolamine. Prepared formulations were evaluated for physical appearance, pH, spreadability, viscosity and homogeneity, skin irritation on animal model (rabbit), and anti-inflammatory activity using carrageenan-induced rat paw edema model on albino Wistar rats of either sex (150–200 g). Change in edema volume of the rat hind paw was measured, and percent inhibition was calculated. ICH guidelines have followed for stability studies. Results: Results reveal that gel showed good appearance, homogeneity, and spreadability. Viscosity is ranging between 4200 and 4500 centipoises. All formulations have shown no skin irritation to animals. Formulations F4 and F5 significantly inhibited the inflammation to the extent of 56.66%, 61.66% at 3 h and 59.21%, 63.15% at 4 h, respectively, while the reference drug reduced the inflammation by 66.66% at 3 h and 76.31% at 4 h. The preparation was stable under normal storage conditions and did not produce any skin irritation, i.e., erythema and edema when applied over the skin on storage. Conclusion: It was concluded that anti-inflammatory effect of F4 and F5 was comparable to standard.

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Study of Anti-Inflammatory and Analgesic Activity of Scorpion Toxins DKK-SP1/2 from Scorpion Buthus martensii Karsch (BmK)

Toxins ◽

10.3390/toxins13070498 ◽

2021 ◽

Vol 13 (7) ◽

pp. 498

Author(s):

Yunxia Liu ◽

Yan Li ◽

Yuchen Zhu ◽

Liping Zhang ◽

Junyu Ji ◽

...

Keyword(s):

Inflammatory Cytokines ◽

Analgesic Activity ◽

Scorpion Toxins ◽

Drg Neurons ◽

E Coli ◽

Mouse Ear ◽

Anti Inflammatory ◽

Rat Paw ◽

Pro Inflammatory Cytokines ◽

Inflammatory Effect

Buthus martensii Karsch (BmK), is a kind of traditional Chinese medicine, which has been used for a long history for the treatment of many diseases, such as inflammation, pain and cancer. In this study, DKK-SP1/2/3 genes were screened and extracted from the cDNA library of BmK. The DKK-SP1/2/3 were expressed by using plasmid pSYPU-1b in E. coli BL21, and recombinant proteins were obtained by column chromatography. In the xylene-induced mouse ear swelling and carrageenan-induced rat paw swelling model, DKK-SP1 exerted a significant anti-inflammatory effect by inhibiting the expression of Nav1.8 channel. Meanwhile, the release of pro-inflammatory cytokines (COX-2, IL-6) was decreased significantly and the release of anti-inflammatory cytokines (IL-10) were elevated significantly. Moreover, DKK-SP1 could significantly decrease the Nav1.8 current in acutely isolated rat DRG neurons. In the acetic acid-writhing and ION-CCI model, DKK-SP2 displayed significant analgesic activity by inhibiting the expression of the Nav1.8 channel. Moreover, DKK-SP2 could significantly inhibit the Nav1.7 current in the hNav1.7-CHO cells.

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