scholarly journals Biochemical Composition of Tea and its Changes under Different Factors

2020 ◽  
Vol 50 (3) ◽  
pp. 404-414
Author(s):  
Nataliia Platonova ◽  
Oksana Belous
Keyword(s):  
Amino Acids ◽  
Ascorbic Acid ◽  
Biochemical Composition ◽  
Raw Materials ◽  
Weather Conditions ◽  
Biologically Active ◽  
Raw Material ◽  
Physiology And Biochemistry ◽  
Krasnodar Region ◽  
The Village

Introduction. The present research featured the antioxidant complex of Russian tea varieties. The research objective was to study the formation patterns of the components responsible for the quality characteristics of tea as a raw material and finished product. The paper describes the changes that the biochemical composition of tea undergoes during the growing season and during processing. The study focused on pigments, vitamins, caffeine, and amino acids. Study objects and methods. The research featured the following varieties of tea grown in the Krasnodar region of Russia: “Kolkhida” (control) and “Sochi”; forms – No. 582, 3823, 855, and 2264. The research was performed on the foundation plantation of collection tea in the village of Uch-Dere (Lazarevsky district of Sochi, Krasnodar region, Russia) and in the laboratory of plant physiology and biochemistry. The study involved traditional and advanced research methods. Results and discussion. The paper introduces some results of a comprehensive analysis of the biochemical composition of various tea sorts. Shoots No. 2264, 3823, and the “Sochi” variety demonstrated high values of ascorbic acid. Form No. 3823 and the “Sochi” variety proved to have a relatively stable content of ascorbic acid. All experimental plants had the highest caffeine synthesis in July (from 24.633 to 28.614 μg/100 g). Processing destroyed caffeine and reduced its amount. The experimental samples of raw materials had eleven amino acids, the largest number being synthesized in May. Processing triggered both general changes in their quantity and varietal differences in the metabolic reactions of amino acid conversion. Conclusion. The tea flushes and finished products differed in all biologically active substances, which is associated with both varietal characteristics and the effect of weather conditions during vegetation.

scholarly journals IMPROVING THE METHOD OF QUANTITATIVE DETERMINATION OF THE AMOUNT OF CAROTENOIDS IN THE RAW MATERIAL “ROSEHIP FRUIT”

2020 ◽  
pp. 131-138
Author(s):  
Vladimir Aleksandrovich Kurkin ◽  
Ol'ga Vladimirovna Sharova ◽  
Polina Valeriyevna Afanaseva
Keyword(s):  
Ascorbic Acid ◽  
Quantitative Determination ◽  
Raw Materials ◽  
Water Soluble ◽  
Biologically Active ◽  
Raw Material ◽  
Test Quality ◽  
Total Carotenoids ◽  
Long Time

The fruits of wild rose (Fructus Rosae) have been widely used in both traditional and officinal medicine for the long time. The pharmacological effect of the Rosehip medicine is related to the content of such biologically active compounds as: water-soluble (ascorbic acid) and fat-soluble (carotenoids) vitamins, as well as flavonoids (astragalin, isoquercitrin, tiliroside). Such medicine as Holosas, Carotolin and Rosehip syrup are obtained from the fruits of this plant. In addition, wild rose fruits are part of the various fees from which patients could prepare water infusions at home themselves. Regulatory documentation provides for the analysis of Rosehip raw materials three parameters: the content of ascorbic acid, the total carotenoids and flavonoids. The quantitative content of the total carotenoids in the fruits of the rosehip is regulated for the production of rosehip oil and Carotolin. However, in our opinion, the method of quantitative determination of the total carotenoids, described in the State Pharmacopoeia of the Russian Federation of the XIV edition, is too complicated and contains an unreasonably entered coefficient in the formula, leading to an overestimation of the analysis results in 10 times. We have developed the methodic of quantitative determination of the total carotenoids in Rosehip, that include single extraction of raw materials with hexane at room temperature and periodic mixing in the ratio of «raw material : extract» 1 : 30. Investigated solution there was obtained by diluting the hexane extract in the ratio of 2:25 and measured the optical density at wavelength 450 nm. Adjustments have been made to the formula for the calculating of the total carotenoids relating to the removal of the coefficient 10 unjustifiably entered into the pharmacopoeial methodic. It was determined that the content of the total carotenoids calculated on ß-carotene varied from 10.15 to 74.5 mg%. The metrological characteristics of the methodic indicate that the error of a single determination of the total carotenoids in the rosehip fruits with a 95% confidence level is ± 3.57%. Based on the results of the studies, a new test quality indicators “content of the total carotenoids at least 30 mg%” was substantiated instead of 300 mg%.

Prospects for the use of Chrysanthemum coronarium (L.) in medicinal crop production

2020 ◽  
Author(s):  
M.I. Tarasova ◽  
Keyword(s):  
Amino Acids ◽  
Ascorbic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Crop Production ◽  
Biologically Active ◽  
Raw Material ◽  
Active Compounds ◽  
Chrysanthemum Coronarium ◽  
Medicinal Crop

А brief overview of the relevance of using garland chrysanthemum (Glebionis coronaria (L.) Cass. ex Spach syn. Chrysanthemum coronarium (L.)) as a raw material in the preparation of medicaments is introduced in this article. High contents of micro- and macroelements were found in garland chrysanthemum, as well as other biologically active compounds, of which anthraquinones, flavonoids, carotenoids and ascorbic acid can be considered the main ones, likewise vitamins B, PP and free amino acids.

scholarly journals Scientific Aspects of Identification Criteria for Fruit Distillates

2021 ◽  
pp. 480-491
Author(s):  
Elena Dubinina ◽  
Ludmila Krikunova ◽  
Violetta Peschanskaya ◽  
Marina Trishkaneva
Keyword(s):  
Amino Acids ◽  
Organic Acids ◽  
Biochemical Composition ◽  
Raw Materials ◽  
Raw Material ◽  
Volatile Components ◽  
Higher Alcohols ◽  
Black Currant ◽  
Alcoholic Drinks ◽  
Acid Index

Introduction. Beverages based on fruit distillates belong to elite alcoholic drinks. As a result, there is a high risk of counterfeit. Controlled indicators do not allow identifying distillates by the type of raw material. The research objective was to develop scientifically based identification criteria for fruit distillates. Study objects and methods. The research featured ten fruits and their distillates. It involved three schemes of pre-distillation processing: pulp fermentation, juice fermentation, and pulp fermentation with subsequent maceration. The biochemical composition of raw materials was assessed by the HPLC analysis of mass concentration of sugars, titratable acids, pH, mono- and disaccharides, free organic acids and amino acids, as well as by the sugar-acid index. The concentration of higher alcohols in the distillates was determined using gas chromatography. Results and discussion. The research revealed significant differences in the biochemical composition of raw materials, which made it possible to divide it into groups depending on the methods of pre-distillation processing. The groups can be identified by the ratio of the concentrations of the main higher alcohols: 1-propanol to the sum of isobutanol and isoamylol. The revealed differences were caused by the peculiarities of the ratio of organic acids and amino acids. For the distillates of Cornelian cherry, black currant, cherry-plum, plum, cherry, and apricot, the ratio of 1-propanol to the sum of isobutanol and isoamylol was within the following ranges: 0.02–0.06, 0.08–0.10, 0.30–0.35, 0.47–0.51, 0.55–0.65, and 0.69–0.92, respectively. The method of preparing raw materials for distillation did not affect the values of the identification indicator. Conclusion. The ratio of 1-propanol to the sum of isobutanol and isoamylol could serve as an indicator for the identification of distillates of Cornelian cherries, black currant, cherry-plum, plum, cherry, and apricot. However, it proved useless for distillates of pears, raspberries, tangerines, and mulberries, since its values were within comparable limits. Therefore, the research requires a GC-MS analysis to determine the concentration and ratios of other specific volatile components in other raw materials.

scholarly journals Phytochemical Analysis of Elaeagnus argentea Leaves

2021 ◽  
Vol 10 (3) ◽  
pp. 95-99
Author(s):  
N. A. Salnikova ◽  
Yu. V. Shur ◽  
A. A. Tsibizova
Keyword(s):  
Ascorbic Acid ◽  
Quantitative Determination ◽  
Raw Materials ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Phytochemical Analysis ◽  
Promising Source ◽  
Active Substances

Introduction. Currently, close attention in the field of pharmacy and medicine is directed to the search for new sources of biologically active substances of various origins, including vegetable. The woody plant Elaeagnus argentea is considered as a promising source. Despite its widespread use in the food industry, folk medicine as an anti-inflammatory, restorative, antimicrobial agent, the chemical composition of this plant has not been sufficiently studied.Aim. Qualitative and quantitative determination of the main groups of biologically active substances (BAS) in the raw material (leaves) of Elaeagnus argentea growing on the territory of the Astrakhan region for further development of a methodology for standardizing the raw material of this plant.Materials and methods. The leaves of Elaeagnus argentea were harvested in the spring in the eastern part of the delta of the Astrakhan region (Volga region). Drying of raw materials was carried out in natural conditions. In the study, unified methods were used: to determine the amount of flavonoids and saponins, the spectrophotometric method was used, ascorbic acid - the titrimetric method. For the analysis of flavonoids in the leaves of Elaeagnus argentea, an aqueous-alcoholic solution of 70 % concentration was used as an extractant. The quantitative content of flavonoids was determined in the obtained raw material extract in terms of luteolin-7-glucoside. The amount of saponins in the leaves of Elaeagnus argentea was determined in terms of oleanolic acid. 96 % ethanol was used as an extractant. The quantitative determination of ascorbic acid in the aqueous extract of crushed raw materials was carried out by titrimetry based on the ability to reduce 2,6-dichlorophenolindophenol.Results and discussion. In the pharmacognostic study of biologically active substances in the leaves of Elaeagnus argentea, the content of ascorbic acid was found to be at least 0.32 %, the amount of flavonoids in terms of luteolin-7-glucoside was at least 1.92 %; saponins - 2.38 %; which indicates the need for a more detailed study of the phytochemical composition of other morphological groups of the plant Elaeagnus argentea growing in the Astrakhan region.Conclusion. The data obtained during the study can be used to confirm the quality of raw materials (leaves) of Elaeagnus argentea. A more detailed study of leaves for the presence of other groups of biologically active substances will make it possible to use the obtained data for the development of regulatory documents for medicinal plant raw materials «Elaeagnus argentea leaves».

Technology of kvass using malts special processing

2021 ◽  
pp. 34-37
Author(s):  
Юлия Юрьевна Миллер ◽  
Валентина Ивановна Бакайтис ◽  
Анатолий Анатольевич Орлов ◽  
Татьяна Федоровна Киселева
Keyword(s):  
Amino Acids ◽  
Raw Materials ◽  
Essential Amino Acids ◽  
Biologically Active ◽  
Raw Material ◽  
Human Organism ◽  
Barley Malt ◽  
Plant Raw Materials ◽  
Essential Components ◽  
High Level

Производство натурального кваса брожения предусматривает использование в технологии традиционно ржаного солода ферментированного или неферментированного (реже муки) и ячменного солода. С целью обогащения продукта незаменимыми для организма человека компонентами в рецептуру напитка вводят различное растительное сырье, отличающееся повышенным содержанием витаминов, минеральных или других биологически активных веществ. Нами предложена технология кваса с использованием высокобелковистого зернового сырья - соевого и овсяного солода, отличающихся повышенным содержанием аминокислот в сравнении с другими злаковыми, используемыми в пивобезалкогольной отрасли. Разработаны 2 рецептуры кваса с обязательным содержанием в обоих случаях ячменного и ржаного ферментированного солода и с добавлением в первом варианте соевого солода в количестве 20 % от общей массы зернопродуктов, во втором - овсяного солода в количестве 15 %. Технология кваса предусматривает стадии приготовления квасного сусла настойным способом, сбраживание квасного сусла сухими хлебопекарными дрожжами при температуре 28…30 °С в течение 20 ч, охлаждение и осветление напитков, розлив. Все зерновое сырье в процессе получения на его основе солода на стадии замачивания обрабатывалось комплексом органических кислот, активизирующим физиологические и биохимические процессы зерна при проращивании. Определены основные качественные и значимые технологические показатели исходного сырья, в том числе ферментативная активность, демонстрирующая высокий уровень, для соевого солода дополнительно определен уровень уреазы, находящийся в допустимых нормах и позволяющий ему быть использованным в пищевых технологиях. Готовые напитки имеют высокие показатели по органолептической оценке - внесение альтернативного сырья их не ухудшило, по показателям качества соответствуют требованиям, предусмотренным стандартом, отличаются повышенным содержанием заменимых и незаменимых аминокислот в сравнении с традиционным квасом. The production of natural fermentation kvass involves the use of traditionally fermented or unfermented rye malt (less often flour) and barley malt in the technology. In order to enrich the product with essential components for the human organism, various plant raw materials are introduced into the recipe of the drink, which are characterized by an increased content of vitamins, minerals or other biologically active substances. We have proposed the technology of kvass using high-protein grain raw materials-soy malt and oat malt, which are characterized by an increased content of amino acids in comparison with other cereals used in the beer and non-alcoholic industry. Two kvass recipes have been developed with an obligatory content of barley and rye fermented malt in both cases and with the addition of soy malt in the first version in the amount of 20 % of the total weight of grain products, in the second - oat malt in the amount of 15 %. The technology of kvass provides for the stages of preparation of kvass wort by the tincture method, fermentation of kvass wort with dry baking yeast at a temperature of 28…130 °C for 20 hours, cooling and clarification of drinks, bottling. All grain raw materials in the process of obtaining malt on its basis at the soaking stage were treated with a complex of organic acids that activates the physiological and biochemical processes of grain during germination. The main qualitative and substantial technological indicators of the raw material were determined, including the enzymatic activity, which demonstrates a high level, and the level of urease, which is within permissible standards and allows it to be used in food technologies, was additionally determined for soy malt. According to the organoleptic assessment, produced drinks have high indicators, the introduction of alternative raw materials did not worsen them, in terms of quality they match the requirements provided for by the standard, they are characterized by an increased content of interchangeable and essential amino acids in comparison with traditional kvass.

scholarly journals Biologically active substances of Camellia sinensis in a humid subtropical climate of Russia

10.5219/1440 ◽  
2021 ◽  
Vol 15 ◽  
pp. 360-368
Author(s):  
Oksana Belous ◽  
Natalia Platonova
Keyword(s):  
Amino Acids ◽  
Ascorbic Acid ◽  
Green Tea ◽  
Black Tea ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Subtropical Climate ◽  
New Variety ◽  
Active Substances

We did a comparative analysis of tea and raw tea materials. There is an increase in the content of carotenoids and flavonoids (thearubigins and theaflavins) in June, a decline in July, and August, and consequently a slight increase again in other months. The increase is due to unfavorable conditions – drought during these periods. In raw new variety forms No. 855 and No. 582, as well as in black tea variety form No. 582 (0.09 mg.g-1), we determined the high value of theaflavins (0.10; 0.11 and 0.09 mg.g-1, respectively). The highest content of thearubigins was found in variety forms No. 582 and No. 3823 (1.33 mg.g-1 and 1.17 mg.g-1). Ascorbic acid is significantly disintegrated (on average 96 – 97%) in the production of black tea. In green tea, ascorbic acid disintegrates to a lesser extent, leaving about 13% of its initial amount in the raw material. The dynamics of GPOD activity in a 3-leaf sprout are variety-specific. At the beginning of the growing season (May), the activity of the enzyme was low – in the range of 0.363 to 0.607 g-unit in sec. In June, there is a decrease in activity, which, however, is not significant (p <0.05) and is due to the biological characteristics of the tea culture. In green tea, the ruthine is on average 3 times more than in black tea (on average about 38.09 and 12.12 mg.100g-1, respectively). We have identified 11 amino acids; the highest percentage accounted for proline (from 30 to 70%), valine (17 – 30%), and serine (about 10%). We have identified 11 amino acids in Krasnodar tea, a large proportion of these amino acids has proline, valine, and serine. There was a variation in the content of biologically active substances depending on genotype characteristics. Studies have identified some controversial issues that require explanation and further study.

Technological aspects of alcohol distillates production from non-traditional sugar-raw materials

2021 ◽  
Vol 9 (16) ◽  
pp. 90-97
Author(s):  
Kateryna Danilova ◽  
◽  
Oksana Zavarzina ◽  
Keyword(s):  
Raw Materials ◽  
Biologically Active ◽  
Raw Material ◽  
Material Base ◽  
Growth Substances ◽  
Technological Properties ◽  
Actual Problem ◽  
Cane Molasses ◽  
Yeast Biomass ◽  
Nitrogen Phosphorus

An actual problem of the alcohol industry development is the expansion of the raw material base through the use of new, cheaper types of carbohydrate sources. Recently, glucose-fructose syrup has been of increasing interest to producers of ethanol. Among the producers of craft drinks, in particular rum-based drinks, cane molasses is in increasing demand. The article presents the technological aspects of the fermentation process of nonconventional sugar-containing raw materials on the example of glucose-fructose syrup and cane molasses in ethanol by different yeast strains. It is determined that glucose-fructose syrup and cane molasses are raw materials depleted of nitrogen, phosphorus and growth substances necessary for the yeast activity. To improve the technological properties of raw materials in the molasses, nitrogen, phosphorus nutrition and corn extract as a growth substance shall be added to a mash. Glucose-fructose syrup is of pH 3.68 and of acidity 0.12, which negatively affects the fermentation. It is established that in order to create optimal living conditions for yeast, it is necessary to add substances to the glucose-fructose syrup that will contribute to the safety of development and prevent the pH 3.5. It was determined that the alcohol yield from 1 ton of glucose-fructose syrup is 31.0 dal, from 1 ton of cane molasses is 24.0 dal on the yeast 46ED and 22 dal on the TegaYeast without the use of growth substances and vitamins necessary for the reproduction of yeast biomass. Under conditions of improving the technological properties of the mash from the glucose-fructose syrup and cane molasses by adding biologically active substances promoting the synthesis of yeast biomass, the ethanol content in the fermented mashes increases up to 11.9 12% vol.

scholarly journals TECHNICAL AND TECHNOLOGICAL SOLUTIONS FOR PREPARING FLAX RAW MATERIALS FOR PROCESSING

2021 ◽  
pp. 227-237
Author(s):  
Yu. Вerezovsky ◽  
T. Kuzmina ◽  
M. Yedynovych ◽  
G. Boyko ◽  
N. Lyalina ◽  
...  
Keyword(s):  
Raw Materials ◽  
Hollow Structure ◽  
Weather Conditions ◽  
Raw Material ◽  
Hollow Structures ◽  
Adverse Weather Conditions ◽  
Adverse Weather ◽  
Long Time ◽  
Experimental Researches

The article contains theoretical and experimental researches in the field of the preservation of flax raw material of high moisture content. In the article, factors that are worsening the quality of flax raw material, resulting in non-observance of agrotechnical and technological requirements of preparing, collecting, harvesting the stem material, adverse weather conditions and other factors, are considered. The objective of this paper is to study the influence of preservation agents’ concentrations and of hollow structure device on the quality of flax raw material during long-time storage. In the article, the influence of aqueous preservatives’ concentration, humidity, storage length on the quality of fibrous products, obtained as a result of processing stem material, is analyzed. The influence of the device of hollow structures, as an alternative to preservatives, on the storage process of bast crops stem material was evaluated. The method of flax retted straw storage is described, actions of the main factors influence on the strength of fibers are analyzed. It is demonstrated, that prolongation of flax raw material preservation time can be made by using preservatives without considerable wastes of quality.

scholarly journals PLANT-BASED MILK ANALOGUES IN THE NUTRITION OF PEOPLE WITH NUTRITION-DEPENDENT NON-INFECTIOUS DISEASES

2020 ◽  
Vol 14 (2) ◽  
Author(s):  
N. Pritulska ◽  
I. Motuzka ◽  
A. Koshelnyk ◽  
M. Jarossová ◽  
A. Lacková
Keyword(s):  
Infectious Diseases ◽  
Raw Materials ◽  
Food Products ◽  
Domestic Market ◽  
Biologically Active ◽  
Raw Material ◽  
Fenugreek Seeds ◽  
Product Group ◽  
Wide Range ◽  
Treatment Procedures

A recent tendency in Ukraine is the changes in the structure of food consumption, which are caused by the reduced consumption of some product groups and by their decreasing quality. A persisting problem is that affordable and easy-to-consume food products made with due consideration of the needs of patients with certain categories of diseases can hardly be found on the domestic market. The products present on the domestic market are all imported, available in a limited assortment, very expensive, and not customised to suit all the specific needs of human metabolism. Approaches to the nutrition of patients with certain types of nutrition-dependent and non-infectious diseases have been analysed. It has been established that аn effective component in the diet of patients with non-infectious diseases can be plant-based milk analogues. It has been determined how important it is to use domestic raw materials: it will contribute to expanding domestic production, will help the gross domestic product growth, and will reduce the product’s costs, thus reducing the costs of diagnostic and treatment procedures. It has been established that the assortment of plant-based milk analogues is constantly expanding. The vegetable raw material conventionally used to make this product group has been analysed. It has been shown that fenugreek seeds can be most effectively used to make special food products (in particular, plant-based milk analogues) for the nutritional support of patients with non-infectious diseases. This is feasible due to the availability of the raw material, simple cultivating conditions, the chemical composition of the product (e. g. a wide range of biologically active substances), low costs of the product when it is made from domestic raw materials, and the simple production technology. It is expected that manufacture of milk analogues from fenugreek seeds will widen the assortment of this product group, and satisfy the target consumers’ needs of safe and high quality products that offer an alternative to imports.

scholarly journals AMINO ACID COMPOSITION OF LEAVES OF THREE SPECIES OF ARTEMISIA L. GROWING IN THE ELTON REGION

2021 ◽  
pp. 219-225
Author(s):  
Галина Табаленкова
Keyword(s):  
Amino Acids ◽  
Czech Republic ◽  
Amino Acid ◽  
Dry Weight ◽  
Biologically Active ◽  
Raw Material ◽  
Buffer System ◽  
Amino Acid Analyzer ◽  
Growing Conditions ◽  
High Level

The results of studies of the qualitative composition and quantitative content of amino acids (AAs) in the leaves of three plant species of the genus Artemisia, widespread in the Elton region, were presented. Protein AAs were determined on an AAA T-339 amino acid analyzer (Czech Republic) after hydrolysis of a sample in 6N HCl at 105 °C for 24 h, free AAs – on an AAA-400 amino acid analyzer (Czech Republic) in a lithium buffer system. The protein AAs amount varied from 66 mg / g in A. lerchiana to 113 mg / g dry weight in A. santonica. 17 AAs were found in composition of these species, aspartic and glutamic acids were dominant. The content of free AAs varied from 4.4 mg / g in A. santonica to 8.3 mg / g dry weight in A. pauciflora. 14 AAs have been identified, among them proline was the predominant free AA. The share of proline was 75-81% of the total free AAs. Among the minor components, 3-4 compounds with a content above 2% dominated. The free AAs contain 3 non-proteinogenic ones (ornithine, citruline, and γ-aminobutyric acid). A. lerchiana and A. pauciflora species were similar in protein and free amino acids, probably due to the same growing conditions. A high level of free proline, together with a complex of biologically active substances in Artemisia species, which grow abundantly in the Elton region, allow to consider the possibility of their use as a medicinal raw material.

Sign in / Sign up

Export Citation Format

Share Document