Prospects for the use of Chrysanthemum coronarium (L.) in medicinal crop production

2020 ◽  
Author(s):  
M.I. Tarasova ◽  
Keyword(s):  
Amino Acids ◽  
Ascorbic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Crop Production ◽  
Biologically Active ◽  
Raw Material ◽  
Active Compounds ◽  
Chrysanthemum Coronarium ◽  
Medicinal Crop

А brief overview of the relevance of using garland chrysanthemum (Glebionis coronaria (L.) Cass. ex Spach syn. Chrysanthemum coronarium (L.)) as a raw material in the preparation of medicaments is introduced in this article. High contents of micro- and macroelements were found in garland chrysanthemum, as well as other biologically active compounds, of which anthraquinones, flavonoids, carotenoids and ascorbic acid can be considered the main ones, likewise vitamins B, PP and free amino acids.

scholarly journals Studies of the chemical composition of a healing skin wound in rats, and of the concentrations of some constituents of tissues distant from the healing wound

1967 ◽  
Vol 102 (3) ◽  
pp. 767-773 ◽  
Author(s):  
J Candlish ◽  
N. Chandra
Keyword(s):  
Amino Acids ◽  
Ascorbic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Muscle Protein ◽  
Skin Wound ◽  
Neutral Salt ◽  
Polythene Tube ◽  
Healing Wound ◽  
Muscle Water

1. A skin lesion was made in rats by dorsal incision and the insertion of a polythene tube. 2. Over a period of 25 days after wounding, assays were performed for ascorbic acid, DNA, hydroxyproline, methionine, tryptophan, tyrosine and free amino acids in the lesion tissue. 3. The neutral-salt-soluble proteins of the lesion tissue were fractionated on DEAE-Sephadex, with the separation of fibrinogen and gamma-globulin from a serum protein fraction. 4. Over a period of 20 days after wounding, in wounded rats and in controls, assays were conducted for: ascorbic acid in lens and liver, hydroxyproline, soluble protein, methionine and water in muscle and tendon, and free amino acids in muscle. 5. Relative to controls there was a decrease in lens and liver ascorbic acid, a rise in tendon hydroxyproline, a rise in muscle free amino acids, a fall in muscle protein and a rise in tendon and muscle water.

scholarly journals Lipoic Acid and Ascorbic Acid Affect Plasma Free Amino Acids Selectively in the Teleost Fish Pacu (Piaractus mesopotamicus)

2004 ◽  
Vol 134 (11) ◽  
pp. 2930-2934 ◽  
Author(s):  
Bendik F. Terjesen ◽  
Kwan Park ◽  
Marcelo B. Tesser ◽  
Maria C. Portella ◽  
Yongfang Zhang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Ascorbic Acid ◽  
Lipoic Acid ◽  
Teleost Fish ◽  
Free Amino Acids ◽  
Free Amino ◽  
Piaractus Mesopotamicus ◽  
Plasma Free Amino Acids

scholarly journals Study of Epilobium angustifolium L. amino acids content by HPLC method

2021 ◽  
pp. 85-90
Author(s):  
Halyna Feshchenko ◽  
Oleksandra Oleshchuk ◽  
Liudmyla Slobodianiuk ◽  
Ivanna Milian
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Raw Materials ◽  
Hplc Method ◽  
Raw Material ◽  
Epilobium Angustifolium ◽  
Amino Acids Composition

The use of plant raw materials is one of the areas of modern pharmaceutical science in the production of herbal drugs. The genus Epilobium counts more than 200 species, many species of which are used in traditional medicine. Among the Epilobium species, Epilobium angustifolium is one of the well-known medicinal plants which have been used worldwide in habitual medicine. There is insufficient information in the literature on the biologically active substances of Epilobium angustifolium L. The presence of three major polyphenol groups: phenolic acids, flavonoids, and ellagitannins were identified in E. angustifolium extracts. Traditionally, the infusion of leaves of this plant could be useful for headaches, cold and gastrointestinal disorder. The Epilobium angustifolium L. as an insufficiently studied plant is a promising object of study, including amino acids composition. To assess the relationship between the production of primary metabolites and their possible therapeutic properties, we analyzed the amino acid profile of the plant Epilobium angustifolium used in traditional medicine. The study of compounds generated by plants as a result of defense mechanisms permits an understanding of the molecular mechanism involved in their medicinal properties. The aim. Thus, the aim of the study was to conduct an HPLC analysis of the amino acids of E. angustifolium to establish the prospects for the use of the raw materials in medical and pharmaceutical practice. The results of the current study will be used in further breeding programs aimed to obtain an industrial form of E. angustifolium suitable for pharmaceutical and food applications. Materials and methods. The determination of amino acids composition of Epilobium angustifolium was conducted using Agilent 1200 (Agilent Technologies, USA). Results. The HPLC method identified sixteen free amino acids and seventeen bound amino acids in the Epilobium angustifolium herb. The studies have shown that Epilobium angustifolium L. herb is mainly composed of free amino acids such as L-phenylalanine (1.65 µg/mg), L-glutamic acid (1.51 µg/mg), L-arginine (1.24 µg/mg), L-alanine (0.98 µg/mg) and L-aspartic acid (0.57 µg/mg), which were presents in the greatest amount. The dominant bound amino acids in the studied raw material were L-glutamic acid, L-aspartic acid, L-leucine, and L-alanine, the content of which was 32.37 µg/mg, 10.59 µg/mg, 8.70 µg/mg, and 6.22 µg/mg respectively. Conclusions. Using the HPLC method determined the amino acids in the herb of Epilobium angustifolium L. The concentrations of L-aspartic acid, L-glutamic acid, L-arginine, L-alanine and L-phenylalanine are predominate among free and bound amino acids in the Epilobium angustifolium L. herb. The result shows that Epilobium angustifolium L. is the source of amino acids, so the use of this plant raw material for new remedies is possible in the future

scholarly journals Biochemical Composition of Tea and its Changes under Different Factors

2020 ◽  
Vol 50 (3) ◽  
pp. 404-414
Author(s):  
Nataliia Platonova ◽  
Oksana Belous
Keyword(s):  
Amino Acids ◽  
Ascorbic Acid ◽  
Biochemical Composition ◽  
Raw Materials ◽  
Weather Conditions ◽  
Biologically Active ◽  
Raw Material ◽  
Physiology And Biochemistry ◽  
Krasnodar Region ◽  
The Village

Introduction. The present research featured the antioxidant complex of Russian tea varieties. The research objective was to study the formation patterns of the components responsible for the quality characteristics of tea as a raw material and finished product. The paper describes the changes that the biochemical composition of tea undergoes during the growing season and during processing. The study focused on pigments, vitamins, caffeine, and amino acids. Study objects and methods. The research featured the following varieties of tea grown in the Krasnodar region of Russia: “Kolkhida” (control) and “Sochi”; forms – No. 582, 3823, 855, and 2264. The research was performed on the foundation plantation of collection tea in the village of Uch-Dere (Lazarevsky district of Sochi, Krasnodar region, Russia) and in the laboratory of plant physiology and biochemistry. The study involved traditional and advanced research methods. Results and discussion. The paper introduces some results of a comprehensive analysis of the biochemical composition of various tea sorts. Shoots No. 2264, 3823, and the “Sochi” variety demonstrated high values of ascorbic acid. Form No. 3823 and the “Sochi” variety proved to have a relatively stable content of ascorbic acid. All experimental plants had the highest caffeine synthesis in July (from 24.633 to 28.614 μg/100 g). Processing destroyed caffeine and reduced its amount. The experimental samples of raw materials had eleven amino acids, the largest number being synthesized in May. Processing triggered both general changes in their quantity and varietal differences in the metabolic reactions of amino acid conversion. Conclusion. The tea flushes and finished products differed in all biologically active substances, which is associated with both varietal characteristics and the effect of weather conditions during vegetation.

scholarly journals The influence of growing conditions on the nutrient composition of table grape varieties in Dagestan

2020 ◽  
pp. 7-15
Author(s):  
O. K. Vlasova ◽  
Z. K. Bakhmulaeva ◽  
T. I. Daudova ◽  
S. A. Magadova
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Functional Food ◽  
Free Amino ◽  
Table Grape ◽  
Raw Material ◽  
Table Grapes ◽  
Quantitative Content ◽  
Growing Conditions ◽  
Grape Varieties

The paper presents the results of a study on the content of free amino acids, including essential ones, vitamins C and P, biologically important macro- and microelements in table grapes of the middle ripening varieties Ag raisins, Vezne, Galan, Hamburg Muscat and Samur, cultivated on the plain in Southern Dagestan Region. The qualitative composition and quantitative content of free amino acids were determined by HPLC on an AAA-881 analyzer. Amino acids tryptophan, cystine and methionine were identified by paper chromatography due to the fact that they decompose when exposed to a temperature of 102 ° C in the amino analyzer reactor. The mass concentration of vitamin C was identified titrimetrically, vitamin P was identified colorimetrically (FEK-56M, Russia), the mineral composition of grapes was identified by flame and atomic absorption photometry using Flapho-4 (Germany) and Hitachi-208 (Japan) devices. Each of the varieties had 18 amino acids, including a full set of essential ones. The total number of detected amino acids was 368.5 (Ag raisins) - 279.9 mg / dm3 (Vezne), and irreplaceable ones - from 103.8 (Hamburg muscat) to 132.4 mg / dm3 (Ag raisins). All experimental grape samples contained vitamins C, from 2.6 (Galan) to 3.7 mg / dm3 (Ag raisins) and P - from 78.0 (Samur) to 131.3 mg / dm3 (Galan), as well as physiologically important minerals. Studies have shown that in terms of the amount of free amino acids, including essential vitamins C and P, healthy minerals, such grapes as Ag raisins, Vezne, Galan, Hamburg and Samur Muscat, grown in the south of Dagestan are natural products with functional properties. Eating it fresh contributes to the normalization of metabolic processes in the human body. Additionally, these grapes can be successfully used as a raw material for the production of multicomponent functional food.

BIOLOGICALLY ACTIVE COMPOUNDS CONTENE ASSESSMENT IN CINNAMON ROSE (ROSA MAJALIS HERRM.) FRUITS FROM THE KUZBASS FLORA

2021 ◽  
pp. 47-51
Author(s):  
Egorova I.N. ◽  
Maltseva E.M. ◽  
Bolshakov V.V.
Keyword(s):  
Ascorbic Acid ◽  
Organic Acids ◽  
Acid Content ◽  
Ascorbic Acid Content ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Raw Material ◽  
Material Base ◽  
Active Compounds ◽  
The Rose

Cinnamon rose (Rosa majalis Herrm.) is the one of two species of rose hips growing in the Kemerovo region. The rose hips pharmacopoeial raw material are fruits which used as a vitamin agent. The rose hips pharmacological effect is due to the rich complex of biologically active substances contained in its fruits. Cinnamon rose belongs to the high-vitamin species. Ascorbic acid content can reach 4-14% in it. Due to the high content of organic acids and polyphenols, rose hips and preparations based on it have a fairly strong antioxidant effect. They are often used to prevent vitamin C deficiency, which is especially important for the region local population living in a territory with a harsh continental climate, characterized by long cold winters. Therefore, the rose hips from Kuzbass flora further study is an urgent goal to expand the medicinal plant raw material base and to provide the population of the region with a vitamin product. The study was aimed at assessing some biologically active compounds content (tannins, organic acids, ascorbic acid) in the cinnamon rose fruits growing in the region. Ascorbic acid quantitative determination was carried out by HPLC, organic acids and tannins - by titration. The analysis showed that the ascorbic acid content in the raw materials collected in the Topkinsky district of the region reaches 0,450±0,003 %, organic acids - 3,728±0,051 %, which corresponds to the PI requirements for this type of raw material. Tannins quantitative content reaches 5.76 %. Obtained experimental data comparative analysis with the literature confirmed the fact that one of the main factors affecting the rose hips vitamin content is the geographical factor.

scholarly journals AMINO ACID PROFILE OF PHASEOLUS VULGARIS PODS AND DRY EXTRACT PREPARED OF THEM

2019 ◽  
pp. 40-48
Author(s):  
L. V. Vronska ◽  
A. Ye. Demyd
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Phaseolus Vulgaris ◽  
Free Amino Acids ◽  
Free Amino ◽  
Total Content ◽  
Essential Amino Acids ◽  
Amino Acid Profile ◽  
Raw Material ◽  
Dry Extract

The aim of the research was to study the amino acid profile of the phaseolus vulgaris pods and extract prepared of them. Materials and methods. Five samples of raw material of phaseolus vulgaris pods (erect herbaceous bushes varieties with white seeds) were collected in the Ternopil and Volyn regions, dry extract of phaseolus vulgaris pods was obtained according to previously developed technology. The study of amino acid composition of the raw materials of phaseolus vulgaris pods and extract prepared of them was carried out using a thin-layer chromatography (TLC) and a high-performance liquid chromatography (HPLC). Results and discussion. The better separation of amino acids in TLC-research of the raw material of phaseolus vulgaris pods was observed in the system of solvents isopropanol - formic acid - water (40: 2: 10). As a result of the study, aspartic and glutamic acids, glycine, valine, tyrosine and leucine were identified. The amino acid profile of the studied samples of raw material is quite homogeneous in composition: 7 essential amino acids (histidine, threonine, valine, methionine, phenylalanine, isoleucine and leucine) and 8 non-essential amino acids (aspartic and glutamic acids, arginine, serine, glycine, alanine, tyrosine and proline); lysine was found among the bound amino acids in the 4th sample of raw material. Proline predominates in all samples of raw materials among free amino acids. Among the bound amino acids the content of glutamic acid, which is the product of the glutamine hydrolysis, is the highest. The content of glycine, serine and alanine is also high. Among the essential amino acids, leucine, phenylalanine, histidine, threonine, isoleucine, valine were determined in content descending order. The quantitative determination of amino acids in the extract of phaseolus vulgaris pods proved that the content of proline was the highest (12.47 mg / g); the content of some compounds was also high: aspartic (5.41 mg / g) and glutamic (3.41 mg / g) acids, arginine (5.10 mg / g; both in free and bound forms), glycine (1.02 mg / g) and serine (1.04 mg / g). Among the essential amino acids in the extract, the amount of valine (0.80 mg / g), phenylalanine (0.67 mg / g), threonine (0.66 mg / g), leucine (0.63 mg / g) and isoleucine (0.58 mg / g) was a little different. The total content of amino acids in the extract was 3.2%. Conclusions. 1. The amino acid profile of five samples of phaseolus vulgaris pods was studied by the HPLC method. It has been established that the composition is quite homogeneous, and the total content varies within 0.7-1.1%. 2. In the dry extract of phaseolus vulgaris pods the content of 5 essential and 7 non-essential amino acids has been determined. The content of free amino acids in the extract is 0.52%; the total content of free and bound amino acids is 3.2%. 3. When studying the stability and establishing the shelf life of the dry extract of phaseolus vulgaris pods, it is necessary to take into account the presence of free amino acids and protein substances.  

scholarly journals OPTIMIZATION OF PARAMETERS OF FERMENTOLYSIS OF PROTEINS IN THE COMPOSITION OF SERUM-PROTEIN CONCENTRATE

2019 ◽  
Vol 12 (4) ◽  
Author(s):  
N. Tkachenko ◽  
L. Lanzhenko ◽  
D. Skripnichenko ◽  
N. Kuprina ◽  
А. Hanicheva
Keyword(s):  
Amino Acids ◽  
Serum Protein ◽  
Free Amino Acids ◽  
Free Amino ◽  
Serum Proteins ◽  
Raw Material ◽  
Short Chain ◽  
Optimal Parameters ◽  
Cosmetic Products ◽  
Nanofiltration Concentrate

The food industry is a strategic industry that works quite steadily even during periods of economic crises, providing food security to any state, and is a source of raw material for other industries with a high potential for development, for example, for the production of cosmetics. The modern cosmetics market is represented by various cosmetic products, often expensive, but not always made from natural ingredients. Therefore, the search for the newest ingredients for the production of natural cosmetics on the basis of domestic raw materials is an urgent task of the present. Ingredients from milk serum can be used for the production of natural cosmetics that in large quantities is obtained in milk processing enterprises and often remains unprocessed. Whey protein concentrations can be a source of short-chain peptides and free amino acids for the production of various cosmetic products. The process of fermentolysis of serum proteins in nanofiltration concentrate KSB-65 with the content of dry matter of 20% using neutral peptidase C from the domestic producer at a temperature of 40 ºС with the duration of the process varying from 1 to 5 hours, the content of peptidase – from 0,5 to 2,0 U/g. It is established that the optimal parameters of the fermentolysis of serum proteins in KSB-65 are as follows: temperature 40 º C, neutral peptidase C content – 0.78 U/g, duration of fermentolysis – 3.17 hours. With optimal parameters of the fermentolysis process, the hydrolyzate of the nanofiltration concentrate KSB-65 contains the maximum amount of short chain peptides (57.03 mg/cm3) and a high concentration of free amino acids (54.66 μg/cm3). Recommendations for the further use of serum protein hydrolyzate obtained using the recommended optimal parameters of the enzyme production process from the nanofiltration concentrate KSB-65, in the manufacture of cosmetic products, including with anti-age effect, and hydrolyzates of proteins enriched with probiotic cultures of lactam bifidobacteria or their lysates.

Sign in / Sign up

Export Citation Format

Share Document