Dietry Impact of Normal and Germinated Barely Grains Products on body weight and Lipid Profile of Diabetic Rats

Aims: Arganimide A (4,4-dihydroxy-3,3-imino-di-benzoic acid) is a compound belonging to a family of aminophenolics found in fruit of Argania spinosa. The purpose of this study was to investigate the glucose and lipid lowering activity of Arganimide A (ARG A). Methods: The effect of a single dose and daily oral administration of Arganimide A (ARG A) on blood glucose levels and plasma lipid profile was tested in normal and streptozotocin (STZ) diabetic rats at a dose of 2 mg/kg body weight. Results: Single oral administration of ARG A reduced blood glucose levels from 26.50±0.61 mmol/L to 14.27±0.73 mmol/L (p<0.0001) six hours after administration in STZ diabetic rats. Furthermore, blood glucose levels were decreased from 5.35±0.30 mmol/L to 3.57±0.17 mmol/L (p<0.0001) and from 26.50±0.61 mmol/L to 3.67±0.29 mmol/L (p<0.0001) in normal and STZ diabetic rats, respectively, after seven days of treatment. Moreover, no significant changes in body weight in normal and STZ rats were shown. According to the lipid profile, the plasma triglycerides levels were decreased significantly in diabetic rats after seven days of ARG treatment (p<0.05). Moreover, seven days of ARG A treatment decreased significantly the plasma cholesterol concentrations (p<0.001). Conclusion: ARG A possesses glucose and lipid-lowering activity in diabetic rats and this natural compound may be beneficial in the treatment of diabetes.

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Amelioration of histological changes and associated metabolic abnormalities by a combination of Morinda lucida and metformin in diabetic rats

The Journal of Phytopharmacology ◽

10.31254/phyto.2018.7304 ◽

2028 ◽

Vol 7 (3) ◽

pp. 253-256

Author(s):

Atanu FO ◽

◽

Momoh S ◽

Ugwu CE ◽

Ameh O ◽

...

Keyword(s):

Body Weight ◽

Lipid Profile ◽

Diabetic Complications ◽

Combined Treatment ◽

Diabetic Rats ◽

Atherogenic Index ◽

Positive Outcomes ◽

Metabolic Abnormalities ◽

Histological Changes ◽

Histological Studies

This work investigates the ability of Morinda lucida and co-administration of Morinda lucida/metformin in the control of biochemical and histological changes in alloxan-induced diabetic rats. Alloxan diabetic rats were treated with 200 mg/Kg body weight of Morinda lucida leaves extract, 1 mg/Kg BW of metformin or a combination of the two treatments for 28 days. Results of the studies revealed that Morinda lucida leaves extract significantly improved lipid profile and kidney function in diabetic rats. These positive outcomes were enhanced by combined treated with Morinda lucida leaves extract and metformin. Furthermore, the calculated atherogenic index of treated animals were close to those of normal rats as opposed to diabetic rats. Similarly, histological studies showed that Morinda lucida leaves extract and metformin administered together or singly, ameliorated damages in pancreas and kidneys from alloxan diabetic rats. It can therefore be inferred that combined treatment with Morinda lucida leaves extract and merformin could improve the potency of Morinda lucida leaves used in the management of diabetic complications

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Treatment of Diabetic Condition in Streptozotocin Induced Diabetic Wister Rats Using Food Blends Such as Unripe Plantain, Soybean and Ginger

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2019/v25i130067 ◽

2019 ◽

pp. 1-12

Author(s):

I. Iwanegbe ◽

M. Suleiman ◽

A. Jimah

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Blood Glucose Level ◽

Lipid Profile ◽

Cell Volume ◽

Glucose Level ◽

Normal Control ◽

Packed Cell Volume ◽

Diabetic Rats ◽

Diabetic Patients

Aims: To investigate the effect of food blends (plantain, soybean and ginger) on the blood glucose, lipid profile and haematological indices on streptozotocin induced diabetic rats. Methodology: A total of 35 rats of mean body weight 219.07 g separated into7 groups (5 per group) where induced by a single intraperitoneal (I.P) injection of streptozotocin (0.1 g dissolved in 5 ml of freshly prepared sodium citrate buffer 0.1 M, pH 4.5) at a dose of 40 mg/kg body weight after fasting for 12 hours and fed with flours/blends. The flours were produced from plant materials for different treatments/blends (blend A=100% unripe plantain, B=80% unripe plantain, 14% soybean, 6% ginger, C=70% unripe plantain, 26% soybean, 4% ginger, D= 60% unripe plantain, 38% soybean, 2% ginger, E= 50% unripe plantain, 50% soybean) and the phytochemicals and minerals content were determined. Blood glucose was determined at 5 days interval for 25 days. Diabetes was confirmed in rats with blood glucose concentrations >200 mg/dl. After 25 days rats were anaesthetized with chloroform vapour and blood samples collected by cardiac puncture for haematology and lipid profile determination. Results: The results showed that unripe plantain, soya beans and ginger in adequate proportion(C=70% unripe plantain, 26% soybean, 4% ginger or D= 60% unripe plantain, 38% soybean, 2% ginger) could help to reduce blood glucose, improve haematological parameters and lipid profile. Significant reduction was observed in the blood glucose level of rats fed blends C and D from 286 to 85 mg/dl and 307 to 90 mg/dl respectively at the end of experiment. These results also demonstrated that the inclusion of ginger at 6% causes rise in blood glucose level. Total cholesterol (TC) increased in all the blends. However, the lowest concentration of TC was observed in blends C and D. The highest packed cell volume (60%) and Haemoglobin (20 g/dl) level observed in rats fed blend C was significantly higher than the normal control fed conventional feeds. The increase in packed cell volume (PCV) (50%) and Hb (17 g/dl) in diabetic rats demonstrated that the formulated blend C was able to raise PCV and Hb above 50% and 17 g/dl (Normal control NC) respectively. Significant increase (P<0.05) in low density lipoprotein cholesterol (LDLc) was also observed in all the blends with blend C having the least (4.0 mg/dl) close to NC (2.0 mg/dl). Conclusion: From the results it is evident that blend C will manage and improve the health status of diabetic patients.

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TO EVALUATE THE ANTI-ATHEROSCLEROTIC POTENTIAL OF QUERCETIN IN ALLOXANINDUCED DIABETIC RATS FED WITH HIGH-FAT DIET

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i3.23056 ◽

2018 ◽

Vol 11 (3) ◽

pp. 379

Author(s):

Biplav S ◽

Sindhura G ◽

Shivalinge Gowda K P

Keyword(s):

Body Weight ◽

Lipid Profile ◽

High Fat Diet ◽

Density Lipoprotein ◽

Diabetic Rats ◽

Group Iv ◽

Lipoprotein Cholesterol ◽

Histopathological Study ◽

High Fat ◽

Group Iii

Objective: The main aim of the present study is concerned with the evaluation of anti-atherosclerotic potential of quercetin in alloxan-induced diabetic rats fed with high-fat diet (HFD).Methods: Atherosclerosis (AS) is the major cause for many of the cardiovascular disease, and it is accelerated in the presence of diabetes mellitus and causes profound alterations in the lipid profile. The method used for the induction of AS was using HFD for 60 days. In this study, rats were divided into four groups (n=6). Group I served as normal control, Group II alloxan (120 mg/kg b.w i.p)-treated diabetic rats, Group III received quercetin (50 mg/ kg b.w p.o), and Group IV received atorvastatin (10 mg/kg b.w p.o) along with alloxan (120 mg/kg b.w i.p) on the 1st day of the days of the study period. AS was induced in Group II, Group III, and Group IV rats by feeding them with HFD from the 1st day to 60th day. The body weight, feed intake was measured daily. The blood was withdrawn from retro-orbital plexus, and the serum was used for the estimation of lipid profile (total cholesterol [TC], triglycerides [TGs], low-density lipoprotein cholesterol [LDL-C], very LDL-C [VLDL-C], and high-density lipoprotein cholesterol [HDL-C]). After scarification under overdose of ketamine, the histopathological study of aorta was carried out.Results: The results showed that the quercetin-treated rats showed a decrease in body weight gain, decreased levels of TC, TGs, LDL-C, and VLDL-C, and increased levels of HDL-C were observed in Group III rats when compared to alloxan-induced diabetic rats fed with HFD (Group III). The histopathological study of aorta showed no development of plaques and of foam cells.Conclusion: From this study, it can be calculated that quercetin has anti-atherosclerotic activity as it significantly altered overall lipid profile in diabetic rats fed with HFD. This activity may be attributed to its antioxidant, inhibition of HMG-CoA reductase activity of quercetin.

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Effects of Spirulina platensis on alloxan induced diabetic rats

Progressive Agriculture ◽

10.3329/pa.v29i2.38300 ◽

2018 ◽

Vol 29 (2) ◽

pp. 139-146

Author(s):

SMAK Hussaini ◽

MI Hossain ◽

MS Islam ◽

K Rafiq

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Oral Administration ◽

Lipid Profile ◽

Spirulina Platensis ◽

Profile Analysis ◽

Diabetic Control ◽

Diabetic Rats ◽

Male Rats ◽

Control Group

The study was undertaken to investigate the effects of Spirulina platensis on body weight, blood glucose and lipid profile on alloxan induced diabetic rats. Fifteen Long Evans male rats at six weeks of age were used for the experiment. Diabetes was induced by intraperitoneal injection of alloxan@ 150 mg/kg and experiment was carried out for a period of 6 weeks. Rats were divided into three groups of 5 rats each. The groups were i)healthy control, ii) diabetic control with both receiving rats’ pellet as supplement, and iii) diabetic treated with oral administration of Spirulina platensis @ 400 mg/kg bwt. Results from the study showed that Spirulina platensis reduced blood glucose significantly (P<0.01) and increased body weight significantly (P<0.05) in contrast to diabetic control group after 6 weeks. Lipid profile analysis with Spirulina platensis @ 400 mg/kg showed that total cholesterol, plasma triglycerides, and LDL were lowered significantly compared to diabetic groups in contrast to HDL levels which were increased following the use of Spirulina platensis. Our findings showed that oral administration of Spirulina platensis could reduce the adverse effect of alloxan induced diabetes in rats. Based on this research it can be concluded that Spirulina platensis has antihyperglycemic and hypolipidimic effects in alloxan induced diabetic rats.Progressive Agriculture 29 (2): 139-146, 2018

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Effect of Ethanolic Extract of Fruits of Eriobotrya japonica on Lipid Profile and Body weight in Streptozotocin Induced Diabetic Rats

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2019.110101 ◽

2019 ◽

Vol 11 (1) ◽

Author(s):

Sabeeha Shafi ◽

Nahida Tabassum

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Lipid Profile ◽

Ethanolic Extract ◽

Diabetic Rats ◽

The Body ◽

Eriobotrya Japonica ◽

Control Group ◽

Glucose Levels ◽

Blood Glucose Levels

Eriobotrya japonica locally called as loquat in Kashmir has been studied in various parts of the world but little work has been reported on Kashmiri loquat. The chemical nature of fruits and vegetables offers a great diversity of biological properties and plays an important role in the field of pharmacology. There is a quest for newer drugs with few adverse effects and this poses a challenge for the development of new drugs. The study was undertaken to study the activities of ethanolic extract of Eriobotrya japonica fruits in streptozotocin induced diabetic rats. The phytochemical screening of the plant was also done. The animals were divided into five groups. Normal Control group received only the vehicle. Toxic group included those animals in which diabetes was induced by streptozotocin. The 3rd group was those animals which received streptozotocin and standard antidiabetic drug-glibenclamide. 4th group included those diabetic animals which received 50 mg/kg b.w dose of fruits of Eriobotrya japonica. 5th group animals included those diabetic animals which received 100 mg/kg b.w of the plant extract. The biochemical parameters that were evaluated were blood glucose levels and lipid profile tests. The body weight was also checked. Histopathology of pancreas was also done. The results showed significant decrease in blood glucose levels, lipid profile tests in animals treated with different doses of the plant extracts. Histopathology of pancreas also showed positive results.

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Protective role of Pandanus tectorius Parkinson ex Du Roi in diabetes, hyperlipidemia, liver and kidney dysfunction in alloxan diabetic rats

Clinical Phytoscience ◽

10.1186/s40816-021-00279-z ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Saima Sundus ◽

Khan Hira ◽

Nida Sohail ◽

Habiba ◽

Amna Tariq ◽

...

Keyword(s):

Body Weight ◽

Lipid Metabolism ◽

Lipid Profile ◽

Liver Enzymes ◽

Water Extract ◽

Ethanol Extract ◽

Diabetic Rats ◽

Kidney Dysfunction ◽

Pandanus Tectorius ◽

Different Parts

Abstract Background Diabetes mellitus (DM), an endocrine disease is characterized by increased level of blood glucose. Diabetes may affect other functions of the body and organs including, renal function and lipid metabolism. Pandanus tectorius (P. tectorius) Parkinson ex Du Roi is a small tree used in folk medicine in treatment of many diseases. Methods Diabetes in rats was induced by intraperitoneal injection of alloxan at a dose of 100 mg/kg body weight for 3 days. Water and ethanol extracts of root, stem and leave of P. tectorius were given to rats at 200 mg/kg body weight at day 3. Rats were sacrificed on day 4 after 12 h fasting. Serum glucose and other biochemical parameters like liver enzymes; [alanine aminotransferases (ALT), aspartate aminotransferases (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH)], kidney parameters (urea & creatinine), lipid profile [total cholesterol, HDL and LDL cholesterol and triglycerides] and coronary artery risk index (CRI) were estimated in blood serum. Antioxidant potential, polyphenol content and phytochemical constituents in different parts of P. tectorius were also determined. Results Results showed that water extract of stem and ethanol extract of root of P. tectorius produced maximum fall in sugar level of alloxan diabetic rats. Extracts of all three plant parts significantly dropped the increased level of urea and creatinine with maximum reduction in creatinine was observed by water extract of root. P.tectorius extracts showed positive effect on liver enzymes and lipid profile by bringing them closer to normal range in comparison to alloxan diabetic control rats. Phytochemical screening indicated presence of flavonoids, alkaloids, tannins and saponin in P. tectorius. In DPPH free radical scavenging test the highest antioxidant potential was found in water extract of roots, while ethanol extract of stem also showed good activity. Conclusion The study demonstrated that different parts of P.tectorius have potential to attenuate diabetes and diabetes related complications like, liver, kidney dysfunction and lipid metabolism.

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Antihyperglycemic Effect ofGinkgo bilobaExtract in Streptozotocin-Induced Diabetes in Rats

BioMed Research International ◽

10.1155/2013/162724 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 44

Author(s):

Daye Cheng ◽

Bin Liang ◽

Yunhui Li

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Lipid Profile ◽

Experimental Period ◽

Food Supplement ◽

Pancreatic Tissue ◽

Diabetic Rats ◽

Adjunct Treatment ◽

Glucose Levels ◽

Wide Range

TheGinkgo bilobaextract (GBE) has been reported to have a wide range of health benefits in traditional Chinese medicine. The aim of this study was to evaluate the antihyperglycemic effects of GBE on streptozotocin- (STZ-) induced diabetes in rats. Diabetes was induced in maleWistarrats by the administration of STZ (60 mg/kg b.w.) intraperitoneally. GBE (100, 200, and 300 mg/kg b.w.) was administered orally once a day for a period of 30 days. Body weight and blood glucose levels were determined in different experimental days. Serum lipid profile and antioxidant enzymes in hepatic and pancreatic tissue were measured at the end of the experimental period. Significant decreases in body weight and antioxidant ability and increases in blood glucose, lipid profile, and lipid peroxidation were observed in STZ-induced diabetic rats. The administration of GBE and glibenclamide daily for 30 days in STZ-induced diabetic rats reversed the above parameters significantly. GBE possesses antihyperglycemic, antioxidant, and antihyperlipidemia activities in STZ-induced chronic diabetic rats, which promisingly support the use of GBE as a food supplement or an adjunct treatment for diabetics.

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Hypoglycemic, Hypolipidemic and Antioxidant Activities of Ocimum gratissimum Leaf Extract on Diabetic Rats

Asian Journal of Biochemistry, Genetics and Molecular Biology ◽

10.9734/ajbgmb/2021/v8i430201 ◽

2021 ◽

pp. 25-40

Author(s):

Na’ima Musa Sani ◽

Abdulhamid Abubakar ◽

Nwagu Jude

Keyword(s):

Diabetes Mellitus ◽

Body Weight ◽

Lipid Profile ◽

Leaf Extract ◽

Antioxidant Activities ◽

Fasting Blood Glucose ◽

Diabetic Rats ◽

Standard Drug ◽

Ocimum Gratissimum ◽

Pancreatic Islets Of Langerhans

Aims: Ocimum gratissimum is a medicinal plant that has been traditionally used in the management of many diseases including diabetes mellitus. The aim of this research was to evaluate hypoglycemic, hypolipidemic and antioxidant activities of Ocimum gratissimum leaf extracts on diabetic rats. Study Design: Mention the design of the study here. Place and Duration of Study: Department of Biochemistry, Faculty of Life Science, Kebbi State University of Science and Technology Aliero, Kebbi State, Nigeria. Between february 2021 and June 2021. Methodology: The phytochemical screening was carried out using standard procedures. The extract was administered orally (100, 200 and 400 mg/kg body weight, for 21 days) to alloxan-induced diabetic rats. Hypoglycemic effects, change in body weight, lipid profile and antioxidant activities of diabetic rats treated with the extract were assessed and compared with normal, diabetic control and standard drug treated rats. Histological examination of the pancreas during 21 days of treatment was also carried out. Results: The extract produced a significant reduction in fasting blood glucose level in alloxan-induced diabetic rats. Significant differences were also observed in body weights, serum lipid profile and levels of antioxidant vitamins and enzymes of Ocimum gratissimum methanol extract treated diabetic rats, when compared with diabetic, normal and standard drug treated rats. Histopathological studies of the pancreas showed comparable regeneration of the cells by extract which were earlier necrosed by alloxan. Methanol leaf extract of Ocimum gratissimum exhibit significant hypoglycemic, hypolipidemic and antioxidant activities in alloxan-induced diabetes in rats. The extract could be further processed towards the management of diabetes mellitus. Conclusion: Ocimum gratissimum extract showed a promising good hypoglycemic effect, the extracts also exhibit hypolipidemic and antioxidant activities on diabetic rats. There was regeneration of pancreatic islets of Langerhans. Therefore at acute dose the extracts can serve as an alternative in the management of diabetes mellitus.

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Persea Americana L. (Avocado) Fruit Mesocarp intake in Experimental Diabetic Rats: Impacts and Implication of Mode of Consumption

Nutrition and Food Processing ◽

10.31579/2637-8914/071 ◽

2021 ◽

Vol 4 (7) ◽

pp. 01-06

Author(s):

Magnus Michael Chukwudike Anyakudo ◽

Ifeoluwa Adekunle Adediji

Keyword(s):

Body Weight ◽

Weight Gain ◽

Blood Glucose ◽

Lipid Profile ◽

Wistar Rats ◽

Body Weight Gain ◽

Diabetic Rats ◽

Persea Americana ◽

Avocado Fruit ◽

Mode Of Consumption

Background: Few epidemiological data exist on the effects of the mode of consumption of avocado on diet quality, weight management, and lipoglycemic profile in diabetic condition and other metabolic disease risk factors. Objectives: This study investigated the metabolic, lipoglycemic, and anthropometric impacts of avocado fruit mesocarp intake and the implication of its mode of consumption on body weight gain, lipid profile, glycemic tolerance and control in male diabetic Wistar rats. Method: Twenty one (21) adult male Wistar rats (150-220g) were randomly categorized into three experimental groups (n = 7, each): Diabetic control fed with normal diet (DC); Diabetic rats fed with avocado supplemented diet (DSA); Diabetic rats treated with aqueous mesocarp extract of avocado (DAE). Diabetes was inducted with 150 mg/dL, alloxan monohydrate solution intraperitoneally. Animals were fed according to the experimental design with water ad libitum for six weeks. Body weights and fasting blood glucose (FBG) concentrations were measured twice weekly. LP and OGTT were conducted. Microsoft Excel and statistical SPSS program version 22 were used for data analysis. Results are expressed as mean ± SEM. Comparison between groups were made using Students’t-test and one way ANOVA. Results: Consumption of avocado mesocarp caused significant reduction in mean body weight gain (DSA: 13.75%; DAE: 10.17%; P value < 0.05) and blood glucose concentrations (DSA: 9.48%; DAE: 21.0%; P = 0.002) with significant improvement in glycemic tolerance and lipid profile (DAE > DSA) over the control. Peaked glycemic responses occurred at 30 minutes of glucose challenge in DSA and DAE groups. Conclusion: Avocado fruit mesocarp intake reduced body weight gain and blood sugar with improved lipid profile and glycemic tolerance in experimental diabetic rats while the mode of consumption influenced its potential impacts.

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