scholarly journals Effects of Spirulina platensis on alloxan induced diabetic rats

2018 ◽  
Vol 29 (2) ◽  
pp. 139-146
Author(s):  
SMAK Hussaini ◽  
MI Hossain ◽  
MS Islam ◽  
K Rafiq
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Oral Administration ◽  
Lipid Profile ◽  
Spirulina Platensis ◽  
Profile Analysis ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Male Rats ◽  
Control Group

The study was undertaken to investigate the effects of Spirulina platensis on body weight, blood glucose and lipid profile on alloxan induced diabetic rats. Fifteen Long Evans male rats at six weeks of age were used for the experiment. Diabetes was induced by intraperitoneal injection of alloxan@ 150 mg/kg and experiment was carried out for a period of 6 weeks. Rats were divided into three groups of 5 rats each. The groups were i)healthy control, ii) diabetic control with both receiving rats’ pellet as supplement, and iii) diabetic treated with oral administration of Spirulina platensis  @ 400 mg/kg bwt. Results from the study showed that Spirulina platensis reduced blood glucose significantly (P<0.01) and increased body weight significantly (P<0.05) in contrast to diabetic control group after 6 weeks. Lipid profile analysis with Spirulina platensis @ 400 mg/kg showed that total cholesterol, plasma triglycerides, and LDL were lowered significantly   compared to diabetic groups in contrast to HDL levels which were increased following the use of Spirulina platensis. Our findings showed that oral administration of Spirulina platensis could reduce the adverse effect of alloxan induced diabetes in rats. Based on this research it can be concluded that Spirulina platensis has antihyperglycemic and hypolipidimic effects in alloxan induced diabetic rats.Progressive Agriculture 29 (2): 139-146, 2018

Evaluation of Glucose and Lipid Lowering Activity of Arganimide A in Normal and Streptozotocin-Induced Diabetic Rats

2019 ◽  
Vol 19 (4) ◽  
pp. 503-510 ◽  
Author(s):  
Mohamed Eddouks ◽  
Farid Khallouki ◽  
Robert W. Owen ◽  
Morad Hebi ◽  
Remy Burcelin
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Oral Administration ◽  
Lipid Profile ◽  
Plasma Cholesterol ◽  
Diabetic Rats ◽  
Lipid Lowering ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lowering Activity

Aims: Arganimide A (4,4-dihydroxy-3,3-imino-di-benzoic acid) is a compound belonging to a family of aminophenolics found in fruit of Argania spinosa. The purpose of this study was to investigate the glucose and lipid lowering activity of Arganimide A (ARG A). Methods: The effect of a single dose and daily oral administration of Arganimide A (ARG A) on blood glucose levels and plasma lipid profile was tested in normal and streptozotocin (STZ) diabetic rats at a dose of 2 mg/kg body weight. Results: Single oral administration of ARG A reduced blood glucose levels from 26.50±0.61 mmol/L to 14.27±0.73 mmol/L (p<0.0001) six hours after administration in STZ diabetic rats. Furthermore, blood glucose levels were decreased from 5.35±0.30 mmol/L to 3.57±0.17 mmol/L (p<0.0001) and from 26.50±0.61 mmol/L to 3.67±0.29 mmol/L (p<0.0001) in normal and STZ diabetic rats, respectively, after seven days of treatment. Moreover, no significant changes in body weight in normal and STZ rats were shown. According to the lipid profile, the plasma triglycerides levels were decreased significantly in diabetic rats after seven days of ARG treatment (p<0.05). Moreover, seven days of ARG A treatment decreased significantly the plasma cholesterol concentrations (p<0.001). Conclusion: ARG A possesses glucose and lipid-lowering activity in diabetic rats and this natural compound may be beneficial in the treatment of diabetes.

scholarly journals Anti-hyperglycemic Effects of Diacerein in Alloxan-Induced Diabetes Mellitus in Wistar Albino Rats

2020 ◽  
pp. 107-113
Author(s):  
Arsalan Uqaili ◽  
Samia Siddiqui ◽  
Roomi Aijaz ◽  
Yar Muhammad Nizammani ◽  
Navaid Kazi ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Treated Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Group B ◽  
Group D

Objective: To determine the anti-hyperglycemic effects of interleukin-1 inhibitor (diacerein) in alloxan induced diabetic albino wistar rats. This experimental study was performed at the Department of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam within 6 months from April 2016 to September 2016. Total of 160 adult Albino Wistar Rats having an average of 200 to 300 grams body weights were selected. Animals were categorized into 4 groups as; Group A (n=15): Control rats – receive 0.9% normal saline as placebo Experimental Groups Group B (n=15): Experimental Control (Diabetic rats) - Alloxan50 mg/kg body weight intraperitoneal. Group C (n=15): Diabetic rats + Diacerein (30 mg/kg/day) orally daily. Group D (n=15): Diabetic rats + Diacerein (50 mg/kg/day) orally daily. Animals were kept and treated as per the NIH Guideline for Use and Care of Laboratory Animals. Diabetes mellitus was induced via a single intraperitoneal injection of 50 milligram/kg alloxan monohydrated dissolved in aseptic 0.9% saline. After 72 hours, blood specimens were taken from the caudal vein of the rats and glucose level>200 mg/dL was taken as diabetes. Experimental rats were given diacerein approximately 30 and 50 mg orally for 6 weeks. At the completion of experiment the body weight was measured of each animal by electronic measuring balance and blood sample was taken from each animal of all groups to assess the blood glucose level and HbA1c level. Data were recorded via self-made proforma and analysis was done by using SPSS version 20. Results: Average body weight of Diabetic control (Group B) was 193.33±22.50 grams, which was lower in contrast to Diacerein treated group C 202.47±25.70 grams and significantly lower as compared to Diacerein treated group D as  212.6±23.43 grams. A significant increase in blood glucose levels 182.07±10.63 mg/dl was noted in the Diabetic control (Group B) compared to Diacerein treated group C (110.13± 8.54 mg/dl) and group D (85.87±8.41 mg/dl) (P=0.001). HbA1c was markedly raised in the Group B- diabetic controls, while diacerein treated diabetic rats (groups C and D) showed a significant decrease in HbA1c (P=0.001). Conclusion: It was concluded that Diacerein achieves the Euglycemic state by reducing the levels of blood glucose and glycated hemoglobin (HbA1c) in Alloxan-Induced diabetes mellitus in Wistar Albino Rats.

scholarly journals Extract Ethanol of Poguntano in Alloxan Induced Diabetic Rats

2018 ◽  
Vol 17 (2) ◽  
pp. 251-254
Author(s):  
Sry Suryani Widjaja ◽  
Rusdiana
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Medical Science ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
High Blood Glucose ◽  
Endothelin 1 ◽  
Back Ground ◽  
Male Wistar Rats

Back Ground: Diabetes mellitus is a group of metabolic disease with high blood glucose level above 150 mg/dL over a prolonged period. The elevated of blood glucose, free fatty acid and insulin resistance will cause the endothelial dysfunction, hemostasis disturbances that lead to micro and macrovascular complications. Poguntano (Picria fel-terrae Merr) from family Scrophulariaceae found in most part of Indonesia, has been used as traditional plant for treatment of diabetes.Objective: The purpose of this study was to investigate the effect of extract ethanol of Poguntano in alloxan induced diabetic rats.Method: Fifteen male Wistar rats with body weight of 150-200 gr were given intra peritoneal injection of 150 mg/kg Alloxan to induce diabetes. These were divided into three groups (control diabetic, group given insulin and group given Extract ethanol of Poguntano 200 mg/Kg body weight) and one control normal control. The duration of study was 4 weeks; blood glucose and Endothelin-1 were measured for all groups.Results : Extract ethanol of Poguntano 200 mg showed significant results (p<0.001) in lowering blood glucose in Alloxan induced diabetic rats compared to control diabetic, but did not show superior to insulin group (p=0.892). Endothelin-1 showed statistical significant between group with normal rats and diabetic control rats. (p=0.009) but did not in the other groups. Levels of Endothelin-1 was higher in the diabetic control group with the median 1.95 (1,78- 3.1).Conclusion: In our study we found that extract ethanol of Poguntano showed significant hypoglycemic activity and lowering the Endothelin-1 level in alloxan induced diabetic rats.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.251-254

scholarly journals Effect of Ethanolic Extract of Fruits of Eriobotrya japonica on Lipid Profile and Body weight in Streptozotocin Induced Diabetic Rats

2019 ◽  
Vol 11 (1) ◽  
Author(s):  
Sabeeha Shafi ◽  
Nahida Tabassum
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Lipid Profile ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
The Body ◽  
Eriobotrya Japonica ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels

Eriobotrya japonica locally called as loquat in Kashmir has been studied in various parts of the world but little work has been reported on Kashmiri loquat. The chemical nature of fruits and vegetables offers a great diversity of biological properties and plays an important role in the field of pharmacology. There is a quest for newer drugs with few adverse effects and this poses a challenge for the development of new drugs. The study was undertaken to study the activities of ethanolic extract of Eriobotrya japonica fruits in streptozotocin induced diabetic rats. The phytochemical screening of the plant was also done. The animals were divided into five groups. Normal Control group received only the vehicle. Toxic group included those animals in which diabetes was induced by streptozotocin. The 3rd group was those animals which received streptozotocin and standard antidiabetic drug-glibenclamide. 4th group included those diabetic animals which received 50 mg/kg b.w dose of fruits of Eriobotrya japonica. 5th group animals included those diabetic animals which received 100 mg/kg b.w of the plant extract. The biochemical parameters that were evaluated were blood glucose levels and lipid profile tests. The body weight was also checked. Histopathology of pancreas was also done. The results showed significant decrease in blood glucose levels, lipid profile tests in animals treated with different doses of the plant extracts. Histopathology of pancreas also showed positive results.

Antidiabetic and Hypolipidemic Effects of Extracts of Gymnema Sylvestre in Streptozotocin Induced Type I Diabetes in Rats

2017 ◽  
Vol 12 (3) ◽  
Author(s):  
Nishtha R. Mahida ◽  
G. . Mandali ◽  
Vijaysinh V. Sindha ◽  
S. K. Raval
Keyword(s):  
Blood Glucose ◽  
Lipid Profile ◽  
Type I Diabetes ◽  
Histopathological Examination ◽  
Diabetic Control ◽  
Hypolipidemic Activity ◽  
Control Group ◽  
Type I ◽  
Gymnema Sylvestre ◽  
Altered Glucose

Gymnema sylvestre of the family Asclepiadaceae is one of the most important medicinal plants of the central eco-region. It is popularly known as Gurmar, which means “sugar killer”. Extract of leaves is reported to have tannins, gum, flavonoids, proteins and saponins. It has displayed a wide array of pharmacological activities. This study was aimed to investigate the antidiabetic and hypolipidemic effects of Gymnema sylvestre extract in experimentally induced diabetes in rats. Diabetes was produced in adult Wistar rats with single dose of streptozotocin (STZ) @ 60 mg/kg b.wt. intraperitoneally. After the confirmation of diabetes on 7th day (sugar >200 mg/dl), alcoholic and aqueous extracts of G. sylvestre (400 mg/kg) were administered orally to the experimental rats from 8th day and continued for 42 days thereafter. The antidiabetic and hypolipidemic activity was estimated by measuring blood glucose, lipid profile and histopathological examination of various tissues from all the groups. Administration of STZ resulted in a significant (p less than 0.01) increase in blood glucose and lipid profile and histopathological alterations in Diabetic control group as compared to healthy control group. Gymnema treatment demonstrated significant (p less than 0.01) antidiabetic effect indicated by restoration of blood glucose compared to STZ control group. The study concluded that extracts of Gymnema sylvestre improved the altered glucose and lipid profile in diabetic rats, suggesting that the Gymnema Sylvestre extracts exhibit the antidiabetic and hypolipidemic activity.

scholarly journals Inhibition ofα-Glucosidase by Thiosulfinate as a Target for Glucose Modulation in Diabetic Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Abdulrahman L. Al-Malki
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Hydrolytic Enzyme ◽  
Positive Control ◽  
Diabetic Rats ◽  
Postprandial Hyperglycemia ◽  
Postprandial Blood Glucose ◽  
Control Group ◽  
Albino Rats ◽  
Predisposing Factor

Postprandial hyperglycemia is a predisposing factor for vascular dysfunction and organ damage.α-glucosidase is a hydrolytic enzyme that increases the glucose absorption rate and subsequently elevates blood glucose levels. Garlic (Allium sativumL.) is a rich source of several phytonutrients, including thiosulfinate (THIO). The aim of this study was to evaluate the ability of THIO, a potent inhibitor of intestinalα-glucosidase, to reduce postprandial blood glucose. Male albino rats were randomly assigned to five different groups (n=10/group). Group 1 served as the control group. Groups 2–5 were injected intraperitoneally with a single dose of streptozotocin (STZ) to induce diabetes. Group 2 comprised untreated diabetic rats. Groups 3 and 4 contained diabetic rats that were given THIO orally (20 mg/kg body weight/day and 40 mg/kg body weight/day, resp.). Group 5 was the positive control having diabetic rats treated orally with acarbose (10 mg/kg body weight/day; positive control). Diabetic rats treated with THIO displayed a significant blood glucose reduction (p<0.001and < 0.01 by analysis of variance, resp.) and a significant elevation in insulin compared with that of untreated rats. THIO is an effective noncompetitive intestinalα-glucosidase inhibitor that promotes hypoglycemic action (p<0.001) in STZ-injected rats. THIO is a promising agent for the management of postprandial hyperglycemia.

scholarly journals Treatment of Diabetic Condition in Streptozotocin Induced Diabetic Wister Rats Using Food Blends Such as Unripe Plantain, Soybean and Ginger

2019 ◽  
pp. 1-12
Author(s):  
I. Iwanegbe ◽  
M. Suleiman ◽  
A. Jimah
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Lipid Profile ◽  
Cell Volume ◽  
Glucose Level ◽  
Normal Control ◽  
Packed Cell Volume ◽  
Diabetic Rats ◽  
Diabetic Patients

Aims: To investigate the effect of food blends (plantain, soybean and ginger) on the blood glucose, lipid profile and haematological indices on streptozotocin induced diabetic rats. Methodology: A total of 35 rats of mean body weight 219.07 g separated into7 groups (5 per group) where induced by a single intraperitoneal (I.P) injection of streptozotocin (0.1 g dissolved in 5 ml of freshly prepared sodium citrate buffer 0.1 M, pH 4.5) at a dose of 40 mg/kg body weight after fasting for 12 hours and fed with flours/blends. The flours were produced from plant materials for different treatments/blends (blend A=100% unripe plantain, B=80% unripe plantain, 14% soybean, 6% ginger, C=70% unripe plantain, 26% soybean, 4% ginger, D= 60% unripe plantain, 38% soybean, 2% ginger, E= 50% unripe plantain, 50% soybean) and the phytochemicals and minerals content were determined. Blood glucose was determined at 5 days interval for 25 days. Diabetes was confirmed in rats with blood glucose concentrations >200 mg/dl. After 25 days rats were anaesthetized with chloroform vapour and blood samples collected by cardiac puncture for haematology and lipid profile determination. Results: The results showed that unripe plantain, soya beans and ginger in adequate proportion(C=70% unripe plantain, 26% soybean, 4% ginger or D= 60% unripe plantain, 38% soybean, 2% ginger) could help to reduce blood glucose, improve haematological parameters and lipid profile. Significant reduction was observed in the blood glucose level of rats fed blends C and D from 286 to 85 mg/dl and 307 to 90 mg/dl respectively at the end of experiment. These results also demonstrated that the inclusion of ginger at 6% causes rise in blood glucose level. Total cholesterol (TC) increased in all the blends. However, the lowest concentration of TC was observed in blends C and D. The highest packed cell volume (60%) and Haemoglobin (20 g/dl) level observed in rats fed blend C was significantly higher than the normal control fed conventional feeds. The increase in packed cell volume (PCV) (50%) and Hb (17 g/dl) in diabetic rats demonstrated that the formulated blend C was able to raise PCV and Hb above 50% and 17 g/dl (Normal control NC) respectively. Significant increase (P<0.05) in low density lipoprotein cholesterol (LDLc) was also observed in all the blends with blend C having the least (4.0 mg/dl) close to NC (2.0 mg/dl). Conclusion: From the results it is evident that blend C will manage and improve the health status of diabetic patients.

scholarly journals Hypoglycemic Potential of Dietary Supplementation of Protein Isolate from Fermented Cucumeropsis manii in Streptozotocin Induced Hyperglycemic in Male Wistar Albino Rats

2021 ◽  
pp. 31-42
Author(s):  
A. O. Abiola ◽  
A. O. Iyoribhe ◽  
S. A. Adeniyi ◽  
O. B. Adu ◽  
A. S. Ogunbowale ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Concentration ◽  
Diabetic Control ◽  
Protein Isolate ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Antioxidant Enzyme Activities ◽  
Cvd Risk ◽  
Streptozotocin Induced Diabetes

The effect of Protein isolate from fermented melon seeds (Ogiri Protei Isolates; OPI) of Cucumeropsis manii on blood glucose, lipid profile, and antioxidant enzyme activities in streptozotocin (STZ)-induced diabetic rats was investigated. Thirty Male Wistar rats were divided into five equal groups. GThe first control group with no exposure. The second group of rats with Streptozotocin-induced non-treated diabetes. The 3rd and 4th groups of rats with Streptozotocin-induced diabetes supplemented with Ogiri protein isolates (200, 600 mg/kg in diet). And the 5th group of rats with Streptozotocin-induced diabetes administered glibenclamide in a dose 500 ug/kg in diet [17]. The OPI was administered for 6 weeks. The administration of OPI reduced the blood glucose concentration of the STZ-induced diabetic rats. Sera and hepatic superoxide dismutase, activities of the STZ-induced diabetic rats were significantly (P< 0.05) increased in comparison with the diabetic control rats. Lipid peroxidation of the supplemented OPI diabetic rats was significantly (P< 0.05) decreased in comparison with the diabetic control rats as the administration of OPI to the STZ-induced diabetic rats significantly increased the enzymes’ activities. The concentration of low-density lipoproteins in the OPI supplemented rats was significantly elevated. These data demonstrate that OPI supplements might be beneficial for correcting hyperglycemia but the consumption of OPI can modulate some tissue lipids in a direction not beneficial for CVD risk in patients with diabetes.

scholarly journals BLOOD GLUCOSE LEVELS AND THE MICROSCOPIC STRUCTURE OF KIDNEY WISTAR RAT DIABETES MELLITUS UNDER THE EFFECT OF LAWSONIA INERMIS (LINN.) LEAVES ETHANOLIC EXTRACT

2018 ◽  
Vol 11 (4) ◽  
pp. 257
Author(s):  
Mutiara Indah Sari ◽  
Maya Anjelir Antika ◽  
Dwi Rita Anggraini
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Wistar Rat ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Lawsonia Inermis ◽  
Control Group Design ◽  
Glucose Levels

 Objective: Lawsonia inermis (Linn.) leaves are one of the alternative medicines to treat diabetes mellitus in Indonesia. We investigated the blood glucose level (BGL) of the L. inermis (Linn.) leaves ethanolic extract (LLEE) leaves and evaluated the histopathological alterations in diabetic rats.Methods: This study was an experimental study with posttest - only control group design. Alloxan (120 mg/kg, intraperitoneally)-induced diabetic rats. 35 of Wistar rats (Rattus norvegicus) were divided randomly into five groups, i.e. K: Normal control, P1: Diabetic control, and P2, P3, and P4 (200 mg/kg body weight [BW], 400 mg/kg BW, and 600 mg/kg BW of LLEE, orally) for 28 days. At the end of the treatment, the rats were sacrificed to obtain the kidney for histopathological evaluation using hematoxylin and eosin technique. BGLs were conducted using a glucose meter (GlucoDR).Results: One-way ANOVA test showed that dose 400 mg/kg BW of the LLEE was related to BGL of alloxan-induced diabetic rats (p=0.000). The histopathological of kidney showed glomerular inflammation (GI), epithelial membrane lining degeneration, vascular congestion, and interstitial tubule hemorrhage at diabetic control (P1). Meanwhile, treated with 600 mg/kg BW of LLEE (P4) showed increase cellular regeneration as normal architecture of the kidney.Conclusion: The LLEE at dose 400 mg/kg BW effective decreased BGL and was able to restore the kidney destruction of alloxan-induced diabetic rats at dose 600 mg/kg BW.

scholarly journals Comparative study of the effect of ethanolic extract of swietenia mahagoni seeds with rosiglitazone on experimentally induced diabetes mellitus in rats

2013 ◽  
Vol 39 (1) ◽  
pp. 6-10
Author(s):  
SMMMA Hasan ◽  
MI Khan ◽  
BU Kumar ◽  
MZ Sadeque
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Control Group ◽  
Group B ◽  
Group D ◽  
Swietenia Mahagoni ◽  
Experimentally Induced

The study was performed to compare the blood glucose lowering effect of Swietenia mahagoni seeds with an oral antidiabetic drug, rosiglitazone in experimentally induced diabetic rats. Twentyfour healthy Long Evans Norwegian strain of rats were included in the study and divided into four groups (A, B, C and D) comprising 6 rats each. Group A (control group) received standard rat food for 14 days. Diabetes was induced by a single intraperitoneal administration of alloxan 120mg/kg body weight in Group B, C and D. Group B was given standard food for 10 days and considered as diabetic control. Group C and D were treated with ethanolic extract of Swietenia mahagoni seeds 1000mg/kg and rosiglitazone 10mg/kg orally respectively. Administration of ethanolic extract of Swietenia mahagoni seeds in group C and rosiglitazone in group D produced a significant reduction in blood glucose level as compared to diabetic control (group B). Histological examination of pancreas showed destruction of beta cells in Islets of pancreas in group B whereas retaining of islets and few degranulations of beta cells of pancreas found in group C and group D. The observations and results of the present study provide information that ethanolic extract of Swietenia mahagoni seeds has hypoglycaemic effect in experimentally induced diabetic rats which requires further investigation. DOI: http://dx.doi.org/10.3329/bmrcb.v39i1.15790 Bangladesh Med Res Counc Bull 2013; 39: 6-10

Sign in / Sign up

Export Citation Format

Share Document