Antioxidant and Antibacterial Activities of Rosmarinus officinalis aqueous extract with Study of The Pharmaco-Toxicological Effect

The propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties, mainly attributed to the presence of polyphenols. The composition of propolis depends on time, vegetation, and the area of collection. Total flavonoid and polyphenol contents of aqueous extracts of propolis samples from different areas of Algeria, determined by using aluminum chloride and Folin–Ciocalteu colorimetric methods, were in the range of 3.047 ± 0.004–5.273 ± 0.013 mg/g and 96.833 ± 0.027–458.833 ± 0.0005 mg/g crude extract of propolis, respectively. This study examined the antioxidant and antimicrobial activities of propolis. Aqueous extracts of propolis were obtained in order to evaluate their antioxidant activities by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, β-carotene and electrochemical assays. All tested propolis samples had relatively strong antioxidant activities, which were also correlated with the total polyphenol and flavonoid content present. The percentage of inhibition of lipid peroxidation of linoleic acid emulsion during 24 h varied between 86.04 ± 0.42 and 90.60 ± 3.77% among the tested samples. The highest DPPH radical scavenging activity was observed by ABAL (Ain Abassa Aqueous Extract) with IC50 = 8.49 ± 5.07 10−5 μg/ml, and the lowest was observed by SAL (Setif Aqueous Extract) with IC50 of 21.16 ± 0.0001 μg/ml. The most important antibacterial activity was obtained with Ain Abassa extract; the zones of inhibition obtained for this excerpt vary from 15.22 to 15.5 mm. Followed by the Setif extract with areas of 12.33 to 12.75 mm, the Tizi-Ouzou extract with areas of 10.11 to 11.11 mm. This study will bring an innovation for further studies with regard to the antioxidant and antibacterial properties of the aqueous extracts of propolis. This study corroborates that Algerian propolis is a rich source of natural antioxidants, properties which could be used in the prevention of different diseases, both in humans and in animals.

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The preventive effect of aqueous extract of Rosemary (Rosmarinus officinalis) leaves against the nephrotoxicity of carbon tetrachloride in mice

Archives of Physiology and Biochemistry ◽

10.1080/13813455.2018.1508236 ◽

2018 ◽

Vol 126 (3) ◽

pp. 201-208

Author(s):

Houda Hamed ◽

Salha Boulila ◽

Ferdaws Ghrab ◽

Rim Kallel ◽

Tahia Boudawara ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Aqueous Extract ◽

Rosmarinus Officinalis ◽

Preventive Effect

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An ethno medicinal plant: Antibacterial activities of Stachys lavandulifolia Vahl aqueous extract against common pathogens

Plant Biotechnology Persa ◽

10.52547/pbp.3.1.2 ◽

2021 ◽

Vol 3 (1) ◽

pp. 0-0

Author(s):

Hori Ghaneialvar ◽

Naser Abbasi ◽

Sania Saneei ◽

akram Zangeneh ◽

Mohammad Mahdi Zangeneh ◽

...

Keyword(s):

Medicinal Plant ◽

Aqueous Extract ◽

Antibacterial Activities

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PROTECTIVE ROLE AND ANTIOXIDANT ACTIVITY OF AQUEOUS EXTRACT OF ROSMARINUS OFFICINALIS AGAINST TRICHLOROACETATE-INDUCED TOXICITY IN LIVER OF MALE RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i6.25353 ◽

2018 ◽

Vol 11 (6) ◽

pp. 420

Author(s):

Medhat Mostafa Abozid ◽

Hoda Ea Farid

Keyword(s):

Aqueous Extract ◽

Liver Damage ◽

Protective Role ◽

Rosmarinus Officinalis ◽

Male Rats ◽

Control Group ◽

Albino Rats ◽

Gamma Glutamyl Transferase ◽

Group I ◽

Glutamyl Transferase

Objective: The current study was designed to estimate the potential protective role of the aqueous extract of rosemary (AER) (Rosmarinus officinalis) against trichloroacetic acid (TCA)-created hepatotoxicity in male albino rats.Methods: Forty male albino rats were separated into four groups of ten: Group I served as control; Group II was given AER (200 mg/kg/day) by gavage; Group III received TCA at the dose 50 mg/kg/day, and Group V was treated with AER (200 mg/kg/day) and received TCA (50 mg/kg/day). The experiment was carried out for 2 months.Results: The toxicity of TCA for rats was revealed by an elevation in liver marker enzymes activities (gamma-glutamyl transferase [GGT], alkaline phosphatase [ALP], aspartate transaminase [AST], alanine aminotransferase [ALT]) and conjugated bilirubin (CB) level, and a decrease in albumin and total protein (TP) levels. The TCA administration also caused a significant increase in the activities of catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), and also malondialdehyde (MDA) level in liver tissues. These biochemical effects were accompanied by histological indicators of liver damage. Treatment with ARE recovered the liver damage instigated by TCA, as showed by perfection of liver enzyme markers (GGT, ALT, AST, ALP), CB, TP and albumin; as well as antioxidant parameters (CAT, SOD, GPx) and lipid peroxidation (MDA) and amelioration of histopathology changes in the liver tissues.Conclusion: It could be concluded that AER supplementation for 2 months in TCA-induced toxicity in rats benefited hepatic antioxidant status and improved liver injury and damage in male albino rats exposed to TCA.

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Studies on the toxicological effect of the aqueous extract of the fresh, dried and boiled berries of Solanum aculeastrum Dunal in male Wistar rats

Human & Experimental Toxicology ◽

10.1177/0960327109354545 ◽

2009 ◽

Vol 28 (12) ◽

pp. 765-775 ◽

Cited By ~ 11

Author(s):

OM Aboyade ◽

MT Yakubu ◽

DS Grierson ◽

AJ Afolayan

Keyword(s):

Body Weight ◽

Aqueous Extract ◽

Wistar Rats ◽

Haemoglobin Concentration ◽

Weight Ratio ◽

The Body ◽

Corpuscular Haemoglobin ◽

Toxicological Effect ◽

Male Wistar Rats ◽

Mean Corpuscular Haemoglobin

The toxicological effect of the aqueous extract of fresh, dried and boiled berries of Solanum aculeastrum Dunal at 1, 10 and 25 mg/kg body weight was investigated in male Wistar rats for 28 days. The parameters used were the body weight of the animals and absolute weights of the organs, haematological parameters, renal and liver functional endpoints. The animals gained appreciable weight and showed no signs of clinical toxicity. The dried (DB), boiled dried (BDB), fresh (FB) and boiled fresh berry (BFB) extracts reduced (p < .05) the heart-, liver-and spleen-body weight ratio of the animals whereas that of the lung was not altered. The kidney and testes-body weight ratios were specifically altered by the different extract. All these were not accompanied by any histomorphological changes. The extracts did not alter (p > .05) the levels of RBC, Hb, PCV and albumin of the animals. The platelets were decreased by the DB and FB whereas BFB increased this parameter. The FB and BFB at all the doses also reduced the mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) of the animals. With the exception of the FB where the creatinine and chloride levels decreased, other extracts did not alter the level of these kidney parameters. Only FB increased the levels of uric acid and urea. All the extract decreased the serum alanine aminotransferase (ALT) of the animal. The levels of total protein, globulin, total and conjugated bilirubin were not altered by DB and BDB whereas these indices were increased by FB and BFB. The DB and BDB increased the serum alkaline phosphatase (ALP) activity whereas FB decreased the activity of the enzyme. In contrast, DB and BDB decreased the serum aspartate aminotransferase (AST) activity of the animals whereas FB and BFB increased the activity of the enzyme. The FB and BFB also increased the levels of potassium, magnesium and phosphorus of the animals. Overall, the alterations in the biochemical parameters by the various extracts of S. aculeastrum berries at these doses indicated that the normal functioning of these organs may be adversely affected. However, drying and boiling might reduce the toxic effect of the berries.

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Antibacterial activities and phytochemical screening of crude extract of Carica papaya leaf against selected pathogens

Global Journal of Pure and Applied Sciences ◽

10.4314/gjpas.v26i2.8 ◽

2020 ◽

Vol 26 (2) ◽

pp. 165-170

Author(s):

A.E. Ajiboye ◽

R.A. Olawoyin

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Klebsiella Pneumoniae ◽

Aqueous Extract ◽

Carica Papaya ◽

Antibacterial Activities ◽

Phytochemical Screening ◽

Zone Of Inhibition ◽

Crude Extracts ◽

Test Organisms

Carica papaya commonly known as paw paw belongs to the family of Curcubitaceae and commonly grown in tropical regions. It possesses antimicrobial, antihelmintic and antioxidant properties. The study assessed the antibacterial potency of Carica papaya against Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus. Acetone and aqueous extracts of the leaves of Carica papaya were obtained using standard methods. The antibacterial activity of the extracts was done using agar well diffusion methods. The Minimum inhibitory and Minimum bactericidal concentrations were done using standard procedures. The antibacterial activities of the crude extracts of Carica papaya against the test organisms revealed that acetone extract showed maximum zone of inhibition on Staphylococcus aureus with a diameter of 17.90±0.10mm at 500 mg/ml and the lowest inhibitory effect on Klebsiella pneumoniae with a zone of 6.50±0.50mm at 100 mg/ml, the aqueous extract showed maximum zone of inhibition on Staphylococcus aureus with a diameter of 15.50±0.50mm at 500 mg/ml and the lowest zone of inhibition was on Staphylococcus aureus with a diameter of 6.50±0.50mm at 100 g/ml. The Minimum Inhibitory Concentration of acetone and aqueous extract was 40 mg/ml and 50mg/ml against Klebsiella pneumoniae respectively. The Minimum Bactericidal Concentration of the extracts ranges from 40-60mg/ml. The qualitative phytochemical screening result revealed the presence of tannins, saponnin, alkaloids and steroid. The quantitative phytochemicals revealed 0.70% of flavonoids, 0.48% of alkaloids, 1.02% of tannin, 0.11% of steroids and 1.08% of glycoside. The result obtained revealed that crude extracts of Carica papaya leaves has antibacterial activities against the test organisms. Keywords: Carica papaya, Phytochemical screening, Pathogens, Antibacterial activity

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Efficacy of Aqueous Extract of Pistia Stratiotes (Water Lettuce) Against some Tropical Fish Bacterial

Journal of Nutrition and Food Security ◽

10.18502/jnfs.v6i3.6827 ◽

2021 ◽

Author(s):

Adelakun Kehinde Moruff ◽

Halidu Shafiu Kilishi ◽

Fajobi Enobong Aloysious ◽

Joshua Deji Abiodun

Keyword(s):

Pseudomonas Fluorescens ◽

Aqueous Extract ◽

Aeromonas Hydrophila ◽

Antibacterial Activities ◽

Salmonella Typhi ◽

Tropical Fish ◽

Pistia Stratiotes ◽

Significant Activity ◽

Water Lettuce ◽

Fish Pathogens

Background: The study was undertaken to determine the phytochemical screening and the antibacterial activities of Pistia stratiotes (water lettuce) against some clinically isolated fish pathogens. Methods: The healthy and disease free plants samples were collected from Jebba Upper Basin of River Niger and tested for their antibacterial activities and phytochemical properties. Pistia stratiotes aqueous extract was extracted using sterile distilled water. The Agar diffused methods were used to determine the antimicrobial actions of the plant against fish pathogens of Pseudomonas fluorescens, Escherichia coli, Salmonella typhi, Aeromonas hydrophila, and Vibrio anguillarum. Results: The antibacterial investigation of aqueous extracts revealed the significant activity against these bacterial. Moreover, phytochemical examination of the crude extract showed the occurrence of saponins, alkaloids, tannins, anthraquinones, flavonoids, and catechins, which completely inhibited the growth of V. anguillarum and E. coli. However, Salmonella typhi, Pseudomonas fluorescens, and Aeromonas hydrophila exhibited moderate varying levels of resistance. Conclusion: The finding of this study showed that bioactive resistance from Pistia stratiotes can help disrupt the activities of various fish bacterial in aquaculture practices

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