scholarly journals PROTECTIVE ROLE AND ANTIOXIDANT ACTIVITY OF AQUEOUS EXTRACT OF ROSMARINUS OFFICINALIS AGAINST TRICHLOROACETATE-INDUCED TOXICITY IN LIVER OF MALE RATS

2018 ◽  
Vol 11 (6) ◽  
pp. 420
Author(s):  
Medhat Mostafa Abozid ◽  
Hoda Ea Farid
Keyword(s):  
Aqueous Extract ◽  
Liver Damage ◽  
Protective Role ◽  
Rosmarinus Officinalis ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Gamma Glutamyl Transferase ◽  
Group I ◽  
Glutamyl Transferase

 Objective: The current study was designed to estimate the potential protective role of the aqueous extract of rosemary (AER) (Rosmarinus officinalis) against trichloroacetic acid (TCA)-created hepatotoxicity in male albino rats.Methods: Forty male albino rats were separated into four groups of ten: Group I served as control; Group II was given AER (200 mg/kg/day) by gavage; Group III received TCA at the dose 50 mg/kg/day, and Group V was treated with AER (200 mg/kg/day) and received TCA (50 mg/kg/day). The experiment was carried out for 2 months.Results: The toxicity of TCA for rats was revealed by an elevation in liver marker enzymes activities (gamma-glutamyl transferase [GGT], alkaline phosphatase [ALP], aspartate transaminase [AST], alanine aminotransferase [ALT]) and conjugated bilirubin (CB) level, and a decrease in albumin and total protein (TP) levels. The TCA administration also caused a significant increase in the activities of catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD), and also malondialdehyde (MDA) level in liver tissues. These biochemical effects were accompanied by histological indicators of liver damage. Treatment with ARE recovered the liver damage instigated by TCA, as showed by perfection of liver enzyme markers (GGT, ALT, AST, ALP), CB, TP and albumin; as well as antioxidant parameters (CAT, SOD, GPx) and lipid peroxidation (MDA) and amelioration of histopathology changes in the liver tissues.Conclusion: It could be concluded that AER supplementation for 2 months in TCA-induced toxicity in rats benefited hepatic antioxidant status and improved liver injury and damage in male albino rats exposed to TCA.

scholarly journals Protective Effect of Resveratrol against Hepatotoxicity of Cadmium in Male Rats: Antioxidant and Histopathological Approaches

Coatings ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 594
Author(s):  
Najah M. Al-Baqami ◽  
Reham Z. Hamza
Keyword(s):  
Fossil Fuels ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Gamma Glutamyl Transferase ◽  
Super Oxide Dismutase ◽  
Biochemical Alterations ◽  
Plant Metabolite ◽  
Glutamyl Transferase ◽  
Secondary Plant Metabolite

Cadmium (Cd) is widely used in some industries and emitted from fossil fuels. It is a heavy metal with a number of side effects, including hepatotoxicity. Resveratrol (Rs) is considered an important polyphenol, which is a secondary plant metabolite and has the ability to scavenge free radicals. The study was designed to evaluate the effects of resveratrol on Cd, which induced hepatotoxicity, by the assessment of some histopathological and biochemical alterations. Forty male albino rats were divided into four groups: the 1st group was the control group, the 2nd group was treated with Cd (5 mg/kg), the 3rd group was given Rs (20 mg/kg), and the 4th group was treated with Cd in combination with Rs intraperitoneally for 30 successive days. The results indicate that Cd increased liver enzymes alanine aminotransferase and aspartate aminotransferase (AST and ALT), alkaline phosphatase ALP and gamma-glutamyl transferase (γ-GT) while reducing the total protein level; Cd increased the malondialdhyde (MDA) level while decreasing the levels of other antioxidant enzymes super oxide dismutase, catalase and glutathione peroxidase (SOD, CAT and GPx). Serious congestion and hemorrhage related to the hepatic tissues were noticed in the Cd group, and Rs plays a major role in alleviating histopathological injuries and hepatic oxidative damage. It is clear that Rs has the ability to minimize the hepatotoxicity induced by Cd in male rats.

scholarly journals The protective effect of the aqueous extract of parsley (Petroselinum sativum ) seeds on experimentally – induced oxidative stress in rats

2021 ◽  
Vol 25 (1) ◽  
pp. 154-172
Author(s):  
K. K. Khudiar ◽  
B. N. Abdullah ◽  
K. A. Al-Mzaien
Keyword(s):  
Oxidative Stress ◽  
Drinking Water ◽  
Protective Effect ◽  
Aqueous Extract ◽  
Plasma Cholesterol ◽  
Male Rats ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Albino Rats ◽  
Group I

In this study, the potential protective effect of aqueous extract of parsley (Petroselinum sativum) seeds against hydrogen peroxide (H2O2) – induced oxidative stress in male rats was assessed. Three groups of male albino rats were randomly divided (n=7) and were handled for twenty-eight days as follows: rats in group I served as control; animals in group || were provided with drinking water containing 0.5% H2O2 and those in group III received orally 8 mg/100 gm B.W. of aqueous extract of parsley seeds plus 0.5% H2O2 in drinking water. After four weeks experimental period, a significant increase in lipid peroxidation products (MDA), and decrease in glutathione (GSH) concentrations were observed in plasma, kidney, liver and heart tissues of H2O2 treated animals as compared with the control group. These biomarkers (GSH and MDA) are interrelated and indicate the occurrence of oxidative stress. Plasma total cholesterol (TC) concentration was significantly increased in H2O2 treated rats. By administration of aqueous extract of parsley along with H2O2, plasma and tissue GSH levels were significantly increased while the elevation in MDA level was diminished in plasma and different tissues examined. A decrease in plasma cholesterol concentration was recorded in H2O2 and parsley treated group as compared with the control one and H2O2 treated groups. These results indicate that aqueous extract of parsley have hypocholesterolemic and antioxidant effect.

Effect of aqueous extract of Gangronema latifolium (G. latifolium) (Utazi) on n-acetyl-para-aminophenol (acetaminophen) - induced hepatic injury on Wistar albino rats

2021 ◽  
Vol 20 (2) ◽  
pp. 119-125
Author(s):  
Godwin Delight Chigamezu ◽  
Wilfred Obaalologhi ◽  
Okure Victoria
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Treated Group ◽  
Oral Dosage ◽  
Control Group ◽  
Albino Rats ◽  
Gamma Glutamyl Transferase ◽  
Group 2 ◽  
Wistar Albino Rats ◽  
Glutamyl Transferase

The present study investigated the effect of leaf extract of Gangronema latifolium (G. latifolium) on acetaminophen (APAP) - induced liver injury in Wistar albino rats. In this study, sixty (60) male Wistar albino rats were divided into five (5) groups of twelve (12) rats each. Animals in group 1 served as control group and received a placebo of 0.9% saline solution. Group 2 served as APAP control group, administered with 800 mg/kg body weight of APAP only. Groups 3, 4 and 5 served as the experimental groups and received oral dosage of 800 mg/kg body weight of APAP plus 150 mg/kg, 200 mg/kg and 250 mg/kg body weight of G. latifolium respectively. The results showed that the enzymatic activities of alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (GGT) in the serum were decreased significantly (p ≤ 0.05) in the experimental groups dosed with 150 mg/kg, 200mg/kg and 250 mg/kg of G. latifolium respectively. For 150 mg/kg G. latifolium treated group, ALT decreased from 23.3 ± 7.31 to 9.00 ± 1.52 IU/L, while AST and ALP decreased from 17.6 ± 2.66 to 15.00 ± 1.00 IU/L and 92.8 ± 2.34 to 83.8 ± 7.94 IU/L respectively. In conclusion, the results showed that aqueous extract of G. latifolium has a protective effect on rat liver induced with APAP injury.

A Study on the Effect of Samoa Extract (Cleome droserifolia) on Sperm Quality in Male Albino Rats Exposed to Pyrethroid

2021 ◽  
pp. 39-45
Author(s):  
Nura I. Al-Zail ◽  
Salah F. Kamies
Keyword(s):  
Sperm Quality ◽  
Group Iv ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Protective Agent ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Induced Changes

Pyrethroid cyhalothrin (PC) is an insecticide that is used worldwide for pest control in agriculture and household use. Samoa extract (SE) is a potent antioxidant protecting cells from oxidative stress. The present study investigates the protective and therapeutic effect of SE on PC-induced changes in sperm quality in male rats. Fifty adult male albino rats were divided into five groups: group I: served as control; group II: received PC i.p. only (6.2 mg/kg b.wt.); group III: received SE only (100 mg/kg b.wt., p.o.) for eight weeks; group IV: received SE as a protective agent daily for eight weeks, then followed by the administration of PC (i.p.) three times a week for two weeks; group V: exposed to PC (i.p.) three times a week for two weeks, then treated with the SE daily for 8 weeks. Results showed that PC caused markedly impaired sperm quality (a count, viability, motility, and abnormality). Compared to PC-treated animals, SE in the protective group markedly restored the alteration of sperm indices. However, SE in the curative group was found to be less effective in restoring PC-induced alterations. In conclusion, the data of this study revealed that the SE as a protective agent is more effective than as a therapeutic agent. Keywords: Samoa; Pyrethroid; Sperm quality; Rat

scholarly journals Effect of Testosterone on Lead Acetate Toxicity in Male Albino Rats

2017 ◽  
Vol 2 (2) ◽  
pp. 112-120
Author(s):  
Nazar Mohammed Shareef Mahmood ◽  
Sarkawt Hamad Ameen Hamad ◽  
Dlshad Hussein Hassan ◽  
Karwan Ismael Othman
Keyword(s):  
Lead Acetate ◽  
Toxic Substance ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Central Vein ◽  
Cell Degeneration ◽  
Group I ◽  
Liver And Kidney ◽  
Adverse Influence

The toxicity of lead acetate (L. A.) concerned to public health disruptor due to its persistence in the environment and it has the adverse influence on the human and animal health as well. It causes physiological,biochemical, and neurological dysfunctions in humans. Histologically it has a negative effect on the liver which is considered one of the major target organs where acts as detoxification machine by elimination the toxic substance from the blood in rich with it.  As well as it affects kidneys that are the two of the most filtering organs. Therefore the present study was aimed to investigate the histopathological effect of L.A. on liver and kidney tissues in male rats. Twenty male rats involved in the study were equally and randomly divided into two groups each of them involved 10 animals. Group I (castrated rats) and Group II (control) each group received 80mg/L of lead acetate dissolved in one liter distilled water by drinking for 15 days. Histological sections showed some alterations including abnormal architecture, cell degeneration, nuclear degeneration, hyperchromatic hepatocytes, immune cells, degeneration in tubules, dilation in sinusoids, dilation in central vein of liver increased bowman's space glomerular atrophy degeneration of tubular cells in liver and kidney tissues of rats in castrated rats from control group. But the size of degenerated tissue was more severe in castrated male rats. It was concluded that the castration process could produce a hypogonadism and decreased testosterone which owns many receptors in kidney and liver may produce adverse influence with L.A. administration.

scholarly journals Roles of Moringa oleifera Leaf Extract in Improving the Impact of High Dietary Intake of Monosodium Glutamate-Induced Liver Toxicity, Oxidative Stress, Genotoxicity, DNA Damage, and PCNA Alterations in Male Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Tarfa Albrahim ◽  
Manal Abdulaziz Binobead
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Leaf Extract ◽  
Liver Toxicity ◽  
Monosodium Glutamate ◽  
Male Rats ◽  
Control Group ◽  
Gamma Glutamyl Transferase ◽  
Glutamyl Transferase ◽  
The Impact

It is common for food to be made more palatable through the use of the flavour enhancer monosodium glutamate, also known as vetsin powder. The purpose of the study described in this paper was to explore how vetsin-induced hepatic toxicity, DNA fragmentation, damage, and oxidative stress modifications could be mitigated with moringa leaf extract (MLE). To that end, 40 male rats were separated into four groups: normal control, positive control or MLE, vetsin, and vetsin combined with MLE. Results indicated that, compared to the control group, the levels of serum alanine aminotransferase (ALT), aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), liver malondialdehyde (MDA), DNA damage, injury, PCNA, and P53 expressions were significantly enhanced by the administration of vetsin (P<0.05). However, the vetsin group had significantly reduced levels of albumin, globulin, total protein, liver glutathione (GSH), superoxide dismutase enzyme (SOD), catalase, and glutathione S-transferase (GST) enzyme activities (P<0.05) by comparison to control. Meanwhile, modifications in liver functions, oxidative stress, DNA damage, liver injury, and PCNA expression were alleviated when vetsin was administered alongside MLE. The authors conclude that vetsin may have many side effects and that MLE can ameliorate biochemical changes, oxidative stress, hepatic injury, PCNA, and P53 alterations induced by vetsin administration.

Effect of electromagnetic waves from mobile phone on immune status of male rats: possible protective role of vitamin D

2017 ◽  
Vol 95 (2) ◽  
pp. 151-156 ◽  
Author(s):  
Ola Ahmed El-Gohary ◽  
Mona Abdel-Azeem Said
Keyword(s):  
Vitamin D ◽  
Immune System ◽  
Mobile Phone ◽  
Protective Role ◽  
Vitamin D Supplementation ◽  
Male Rats ◽  
Control Group ◽  
Group I ◽  
Mobile Phone Radiation

There are considerable public concerns about the relationship between mobile phone radiation and human health. The present study assesses the effect of electromagnetic field (EMF) emitted from a mobile phone on the immune system in rats and the possible protective role of vitamin D. Rats were randomly divided into six groups: Group I: control group; Group II: received vitamin D (1000 IU/kg/day) orally; Group III: exposed to EMF 1 h/day; Group IV: exposed to EMF 2 h/day; Group V: exposed to EMF 1 h/day and received vitamin D (1000 IU/kg/day); Group VI: exposed to EMF 2 h/day and received vitamin D (1000 IU/kg/day). After 30 days of exposure time, 1 h/day EMF exposure resulted in significant decrease in immunoglobulin levels (IgA, IgE, IgM, and IgG); total leukocyte, lymphocyte, eosinophil and basophil counts; and a significant increase in neutrophil and monocyte counts. These changes were more increased in the group exposed to 2 h/day EMF. Vitamin D supplementation in EMF-exposed rats reversed these results when compared with EMF-exposed groups. In contrast, 7, 14, and 21 days of EMF exposure produced nonsignificant differences in these parameters among all experimental groups. We concluded that exposure to mobile phone radiation compromises the immune system of rats, and vitamin D appears to have a protective effect.

scholarly journals Comparison between the Antioxidant and Antidiabetic Activity of Fenugreek and Buckthorn in Streptozotocin-Induced Diabetic Male Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Mohammed A. Alsieni ◽  
Haddad A. El Rabey ◽  
Abdulbasit I. Al-Sieni ◽  
Madeha N. Al-Seeni
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Density Lipoprotein ◽  
Negative Control ◽  
Seed Extract ◽  
Male Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Leaf Aqueous Extract

This study is aimed at comparing the antidiabetic and antioxidant potential of fenugreek and buckthorn which are commonly used in modulating diabetes in the Middle East. In this study, the antioxidant and antidiabetic activity of the aqueous extracts of the leaf and seed of fenugreek and buckthorn was tested in streptozotocin-induced diabetic male rats fed with a fat-rich diet for 8 weeks. Thirty-six male albino rats were divided into 6 groups ( n = 6 ); the 1st group was the negative control. Diabetes was induced in the other 30 rats using streptozotocin, which were then divided into 5 groups; the 2nd was the untreated positive diabetic group, the 3rd was treated with fenugreek leaf aqueous extract, the 4th was treated with the fenugreek seed aqueous extract, the 5th was treated with buckthorn leaf aqueous extract, and the 6th was treated with buckthorn seed aqueous extract. The positive control group showed an increase in blood sugar, glycated hemoglobin, liver function enzymes, lactate dehydrogenase, kidney indices, total cholesterol, triglycerides, low- and very-low-density lipoprotein, immunoglobulins, and lipid peroxidation and a decrease in high-density lipoprotein, albumin, and antioxidant activity. The histology of the liver and testes showed severe histopathological alterations. Rats of groups 4-6 that were treated with the aqueous extract of the leaf and seed extract of fenugreek and buckthorn showed improvement of all biochemical and histopathological parameters. The seed extract of fenugreek and buckthorn showed more antioxidant activity than their leaves.

scholarly journals Cardioprotective Effect of Ajwa Date Aqueous Extract on Doxorubicin-Induced Toxicity in Rats

2018 ◽  
Vol 11 (3) ◽  
pp. 1521-1536 ◽  
Author(s):  
Meaad F. Sabbah ◽  
Fawzia Alshubali ◽  
Othman A. S. Baothman ◽  
Mazin A. Zamzami ◽  
Lobna Shash ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Aqueous Extract ◽  
Chemotherapeutic Agents ◽  
Male Rats ◽  
Control Group ◽  
Dna Integrity ◽  
Protective Effects ◽  
Cardiac Enzymes ◽  
Group Iii ◽  
Group I

Doxorubicin (DOX) is one of the most potent and widely used chemotherapeutic agents to treat several malignancies. However, the clinical use of DOX is seriously restricted due to its acute and chronic cardiotoxic side effects This study investigated the protective effect of (Ajwa) date aqueous extract (AJDAE) against doxorubicin-induced cardiotoxicity in rats. Sixty Wister albino male rats (150-200 gms.) were comprised in our study and divided into six equal groups: group I (untreated control), group II, group III, rats were orally received AJDAE (0.75 & 1.5 gm/ kg.bw) respectively, for 4 weeks, rats of groups IV, V and VI were intraperitoneally injected with one dose of doxorubicin (5 mg/kg.bw) at the end of the 4th week of the study to induce cardiotoxicity, rats of groups V & VI were orally received AJDAE (0.75 & 1.5 gm/ kg.bw) respectively. Cardiac enzymes, lipid profile, SOD, GR, GST, GPx, CAT and MDA in rats’ hearts homogenate, urinary 8OHdG as well as DNA integrity and histopathological changes were investigated in all studied rats.Oral administration of AJDAE (0.75 & 1.5 gm/ kg.bw) attenuated the cardiotoxicity of DOX, improved the cardiac enzymes, lipid profile, reduced the urinary 8OHdG and prohibited the depletion of endogenous antioxidants and suppressed lipid peroxidation (MDA). Moreover, AJDAE enhanced DNA integrity. Histological findings showed that AJDAE (0.75 & 1.5 gm/ kg.bw) administration reduced cardiomyocytes alterations, congestion, edema and the intense cellular stress exerted on myocardial fibers as well as restored the cardiomyocytes architecture. Our data showed that AJDAE obviously resulted in protective effects against DOX-induced cardiotoxicity in rat’s heart. It can be concluded that Ajwa date offers a considerable protection against DOX-induced cardiotoxicity.

Toxicological implications of aqueous extract of Bambusa vulgaris leaves in pregnant Dutch rabbits

2009 ◽  
Vol 28 (9) ◽  
pp. 591-598 ◽  
Author(s):  
MT Yakubu ◽  
BB Bukoye ◽  
AT Oladiji ◽  
MA Akanji
Keyword(s):  
Body Weight ◽  
Aqueous Extract ◽  
Clinical Signs ◽  
Exposure Period ◽  
Control Group ◽  
Total Protein Content ◽  
Central Vein ◽  
Gamma Glutamyl Transferase ◽  
Bambusa Vulgaris ◽  
Glutamyl Transferase

Aqueous extract of Bambusa vulgaris L. leaves at 250 and 500 mg/kg body weight was investigated for toxic effects in pregnant rabbits. Apparently healthy, female rabbits (Dutch) weighing between 1.62 and 1.70 kg as previously used in our abortifacient study were paired overnight with male rabbits in ratio 2:1 and those that became pregnant were completely randomized into three groups (A-C). Group A (the control), received orally 1.85 mL/kg body weight (3 mL) of distilled water thrice daily on days 1-9 of pregnancy while groups B and C were treated orally with the same volume corresponding to 250 and 500 mg/kg body weight of the extract. Clinical signs of toxicity were not observed in all the animals during the study. The extract did not significantly alter (p > .05) the serum follicle stimulating hormone and total protein content of the pregnant rabbits throughout the exposure period whereas, the concentrations of luteinizing hormone, progesterone, albumin, globulin, urea and calcium decreased in the serum of the rabbits. At 250 mg/kg body weight, the extract increased kidney alkaline phosphatase (ALP) activity whereas at 500 mg/kg body weight of the extract, the ALP level was similar to the control group. Liver ALP at all doses, as well as the activity of gamma glutamyl transferase (GGT) at 500 mg/kg body weight was reduced. This reduction was accompanied by an increase in serum ALP and GGT at these doses. At 250 mg/kg, the extract increased kidney GGT. Conversely, at 500 mg/ kg, kidney GGT activity decreased. Liver and serum GGT were not altered by the 250 mg/kg. The extract also increased the serum levels of creatinine, uric acid, sodium, potassium and bicarbonate ions as well as total and conjugated bilirubin. In the hepatocytes of extract-treated animals, there was no evidence of necrosis, inflammation, fibrosis and degenerative changes in the central vein and radiating hepatic cords, while the glomerulus and the tubules of the nephrons also remained intact. The alterations in biochemical parameters by the aqueous extract of B. vulgaris leaves suggests adverse effect on the synthetic, secretory, reabsorptive and excretory functions of liver and kidney of the animals. Therefore, the absence of histopathological lesions in the hepatocytes and nephrons implies that histopathological changes are not a sensitive assay for the assessment of tissue damage by the extract.

Sign in / Sign up

Export Citation Format

Share Document