scholarly journals Evaluation of Multi-Drug Resistant E. coli from Drinking Water Sources in a Tertiary Institution

2020 ◽  
pp. 1-9
Author(s):  
Udoh I.P., Iloghalu ◽  
Iloghalu, Ijenwa Amarachi ◽  
Aladenika S.T.
Keyword(s):  
Drinking Water ◽  
Multidrug Resistance ◽  
Water Samples ◽  
Antimicrobial Agents ◽  
Water Sources ◽  
Bottled Water ◽  
Health Concern ◽  
Storage Tanks ◽  
E Coli ◽  
Drinking Water Sources

Infectious diseases control of recent is a major health concern globally due to high increase in number of microorganisms that are resistant to conventional antimicrobial agents. This study aimed at ascertaining the microbiological quality and multiple antibiotic resistance profile of E. coli strains isolated from different sources of drinking water. A total of 136 water samples from different drinking water sources, including the storage tanks (the school and the commercial storage tank), sachet and bottle water were obtained from University of Nigeria Enugu Campus and analyzed. Standard microbiological techniques were employed for bacteria isolation, identification and antibiogram. From the water samples collected 25 E. coli strains were isolated. The school storage tanks account for 60% of the isolates, while bottled water showed no growth. 92% of the E. coli isolated showed resistance to the tested antibiotics. Resistant were higher with Augmentin (64%), Chloramphenicol (48%) and Streptomycin 11 (44%) while most were sensitive to Tarivid and Perfloxacine (100%). Isolates from school storage water sources showed the highest resistance to Augmentin (76.5%) while those from commercial storage water sources showed the highest resistance to Streptomycin (66.7%). Out of the 23 (92%) antibiotics resistant E. coli isolates 18 (78.3%) were multidrug resistance (MDR). The school storage water sources had the highest number of MDR E. coli 14 (77.8%) followed by the commercial storage water sources 4 (22.2%), but the sachet and bottled water had no MDR E. coli. In conclusion, drinking water may potentially contribute to the source of multidrug resistance E. coli in this community

scholarly journals Abundance of pathogenic Escherichia coli, Salmonella typhimurium and Vibrio cholerae in Nkonkobe drinking water sources

2006 ◽  
Vol 4 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Maggy N. B. Momba ◽  
Veronica K. Malakate ◽  
Jacques Theron
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Salmonella Typhimurium ◽  
Vibrio Cholerae ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Water Sources ◽  
E Coli ◽  
Drinking Water Sources

In order to study the prevalence of enteric pathogens capable of causing infection and disease in the rural communities of Nkonkobe, bacterial isolates were collected from several surface water and groundwater sources used by the community for their daily water needs. By making use of selective culture media and the 20E API kit, presumptive Escherichia coli, Salmonella spp. and Vibrio cholerae isolates were obtained and then analysed by polymerase chain reaction assays (PCR). The PCR successfully amplified from water samples a fragment of E. coli uidA gene that codes for β-D-glucuronidase which is a highly specific characteristic of enteropathogenic E. coli, enterotoxigenic E. coli and entero-invasive E. coli. The PCR also amplified the epsM gene from water samples containing toxigenic V. cholerae. Although E. coli was mostly detected in groundwater sources, toxigenic V. cholerae was detected in both surface and groundwater sources. There was a possibility of Salmonella typhimurium in Ngqele and Dyamala borehole water samples. The presence of these pathogenic bacteria in the above drinking water sources may pose a serious health risk to consumers.

scholarly journals Parasitic Contamination of Local Drinking Water Sources in Aba Metropolis, Abia State, Nigeria

2021 ◽  
Vol 26 (1) ◽  
pp. 1-7
Author(s):  
Blessing U. Ejike ◽  
Carmelita C. Ohaeri ◽  
Ebube C. Amaechi
Keyword(s):  
Public Health ◽  
Drinking Water ◽  
Entamoeba Histolytica ◽  
Water Samples ◽  
Giardia Lamblia ◽  
Stream Water ◽  
Water Sources ◽  
Health Concern ◽  
Parasitic Infections ◽  
Drinking Water Sources

Water-borne parasitic infections have become a source of concern in recent times due to the contamination of various water sources. A survey on the parasitic contamination of local drinking water sources in Aba metropolis was carried out between August and October 2018. This was done to evaluate the extent of contamination of these water sources and their public health implications. A total number of eighty (80) water samples were investigated from five (5) major water sources within the study area using the standard sedimentation method and examined for parasite cysts, eggs and larvae. Physical/macroscopic examination was done to determine the pH, colour, odour, temperature and presence/absence of particles. Among the water sources analyzed, stream water samples had the highest rate of parasite contamination 27 (44.3%) followed by river water 14 (22.9%) while the least contaminated was borehole 7 (11.5%). Rainwater was found free from parasitic infestation. The differences in the level of contamination among the water sources analyzed were found to be statistically significant (P<0.05). Five parasites Entamoeba histolytica, Giardia lamblia, Ascaris lumbricoides of Entamoeba coli and Hookworm were observed. Each of the sample sources except rainwater harbored at least one of these. Among these parasites, Giardia lamblia had the highest rate occurrence of 15 (24.6%), followed by Entamoeba histolytica 14 (22.9%) and the least frequently occurring parasite was hookworm 8 (13.1%). Most of the identified water sources are utilized by the populace who lack access to potable water. The detection of protozoan and helminthic parasites in drinking water sources is a serious public health concern, hence the proper implementation of a supply of safe drinking water is vital.

scholarly journals Detection of Virulence Genes and Biofilm Forming Capacity of Diarrheagenic E. coli Isolated from Different Water Sources

Coatings ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1544
Author(s):  
Sadaf Tariq ◽  
Sobia Tabassum ◽  
Sadia Aslam ◽  
Mika Sillanpaa ◽  
Wahidah H. Al-Qahtani ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Water Sources ◽  
Health Concern ◽  
Pcr Analysis ◽  
E Coli ◽  
Drinking Water Samples ◽  
Different Water Sources

Diarrheagenic Escherichia coli (DEC) are associated with frequent incidences of waterborne infections and pose health risk to individuals who contact water for domestic or recreational uses. Detection of DEC pathotypes in drinking water can be used as an indicator of fecal contamination. This study aimed to investigate the occurrence of DEC pathotypes and their capacity to form biofilms in drinking water samples collected from different water sources. In this study, PCR analysis was used to determine the occurrence of four clinically significant virulence genes of diarrheagenic E. coli, eaeA (Enteropathogenic E. coli), stx1, stx2 (Enterohemorrhagic E. coli) and sth (Enterotoxigenic E. coli), in drinking water samples (n = 35) by using specific primers and conditions. PCR amplicons were visualized by using agarose gel electrophoresis. A total of 12/35 (34%) samples were detected as positive for at least one of the four DEC virulence genes and 11/12 (91%) E. coli isolates harbored virulence gene while 1/12 (8%) E. coli isolates harbored none. The eaeA and sth genes were the most detected genes (75%), while stx1 and stx2 genes were least detected genes (66%). Biofilm assay confirmed that ETEC pathotypes can cause damage in enteric walls by attaching and effacing to persist diarrheal conditions. This study indicated that drinking water of different sources is contaminated with potential DEC pathotypes and it can be a source of diarrheal diseases. The amplification of four virulence genes associated with DEC pathotypes (EPEC, EHEC and ETEC) in drinking water demonstrates that potentially virulent DEC pathotypes are distributed in water sources and may be a cause of health concern. There is, therefore, an urgent need to monitor DEC pathotypes in drinking water.

scholarly journals Heavy metal levels in drinking water sources of Chingola District, Zambia

2020 ◽  
Author(s):  
Francis Hamwiinga ◽  
Chisala D. Meki ◽  
Patricia Mubita ◽  
Hikabasa Halwiindi
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Drinking Water ◽  
Ground Water ◽  
Water Samples ◽  
Tap Water ◽  
Water Sources ◽  
Wet Season ◽  
Drinking Water Sources ◽  
Metal Levels

Abstract Background: One of the factors impeding access to safe water is water pollution. Of particular concern is heavy metal contamination of water bodies. This study was aimed at determining the levels of heavy metals in drinking water sources of Chingola District of Zambia. Methods: A cross sectional study was employed. A total of 60 water samples were collected. Thirsty (30) samples were collected in the dry season in the month of October 2016 and another 30 in the wet season in the months of February and March, 2017. For each season 10 water samples were collected from each of the three water sources. i.e. Tap water, Urban ground water sources and Rural ground water sources. Heavy metal analysis was done using Atomic Absorption Spectrophotometer (AAS).Results: This study revealed that the concentrations of Iron, Manganese, Lead, Nickel and Arsenic were beyond maximum permissible levels in various water sources. Combined averages for both dry and wet seasons were as follows: Iron: 2.3, Copper: 0.63, Cobalt: 0.02, Manganese: 0.36, Lead: 0.04, Zinc:3.2, Nickel: 0.03, Arsenic: 0.05. Chromium and Cadmium were below detection limit in all water samples. The median concentrations of iron, arsenic, copper, manganese in drinking water from the Tap, rural and urban ground water sources were different, and this difference was statistically significant (p<0.05). The median concentrations of arsenic, nickel, manganese and cobalt were different between dry and wet season, and this difference was statistically significant (p<0.05).Conclusions: Sources of heavy metals in water seems to be both natural and from human activities. The concentration of heavy metals in different water sources in this study was found to be above the recommended levels. This calls for improvement in water monitoring to protect the health of the public. Therefore, there is need for continuous monitoring of heavy metals in drinking water sources by regulatory authorities.

scholarly journals Is Drinking Water Sources Safe for Drink? A Multicenter Assessment of Fecal Contamination of Drinking Water Sources in Tigray Region, Ethiopia

2019 ◽  
Author(s):  
Goyitom Gebremedhn ◽  
Abera Aregawi Berhe ◽  
Abraham Aregay Desta ◽  
Lemlem Legesse
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Fecal Coliform ◽  
Fecal Contamination ◽  
Water Sources ◽  
Capital City ◽  
Most Probable Number ◽  
Probable Number ◽  
Tigray Region ◽  
Drinking Water Sources

Abstract Background Fecal contamination of drinking water sources is the main cause of diarrhea with estimated incidence of 4.6 billion episodes and 2.2 million deaths every year. Methods A total of 145 water samples of different source type were collected from different areas in Tigray region from August 2018 to January 2019. The water samples from each site were selected purposively which involved sampling of water sources with the highest number of users and functionality status during the study period. Most Probable Number (MPN) protocol was used for the bacteriological analysis of the samples. Results A total of 145 water samples were collected from six zones in Tigray region, Ethiopia from August 2018 to January 2019. The study indicated that 63(43.5%) of the water samples were detected to have fecal coliform which is E.coli. In Mekelle city, which is the capital city of Tigray region, three in five 34(60.7%) of the collected samples were confirmed to have fecal coliform. Water samples from health facilities were 9.48 times [AOR=9.48, 95%CI: (1.59, 56.18)] more likely to have fecal coliform. Water samples from wells were 10.23 times [AOR=10.23, 95%CI: (2.74, 38.26)] more likely to have fecal coliform than water samples from Tap/Pipe. Similarly, water samples from hand pumps were 22.28 times [AOR=22.28, 95%CI: (1.26, 393.7)] more likely to have fecal coliform than water samples from Tap/Pipe. Water samples reported to be not chlorinated were 3.51 times [AOR=3.51, 95%CI: (1.35, 9.13)] more likely to have fecal coliform than water samples from chlorinated sources. Conclusion In this study all water source, including the chlorinated drinking water sources, were found highly contaminated with fecal origin bacteria. This may be mainly due to constructional defects, poor sanitation inspection, poor maintenance, intermittent water supply and irregular chlorination.

scholarly journals Borehole water: a potential health risk to rural communities in South Africa

2018 ◽  
Vol 19 (1) ◽  
pp. 128-136 ◽  
Author(s):  
S. Taonameso ◽  
L. S. Mudau ◽  
A. N. Traoré ◽  
N. Potgieter
Keyword(s):  
Drinking Water ◽  
Rural Communities ◽  
Water Samples ◽  
Rural Areas ◽  
Total Coliform ◽  
Water Sources ◽  
Potential Health ◽  
Point Mapping ◽  
E Coli ◽  
Mapping Tool

Abstract Sporadic outbreaks of diarrhoea in children in the Vhembe rural areas could be an indication of contamination in drinking water sources. In areas where improved water sources are used, not all rural households experience the benefits of these improved water sources. Water samples were collected from boreholes in three wards in the Vhembe District to determine microbiological risks over a 5-month period. A Water Point Mapping tool was used to indicate the borehole distribution. Water samples were taken from each functional borehole and analysed for total coliform and Escherichia coli counts, electrical conductivity, pH and temperature. A multiplex PCR protocol was used for identification of pathogenic E. coli. A total of 125 boreholes were identified of which only 12 were functional. Seven boreholes tested positive for total coliforms and E. coli counts. Four boreholes (33.3%) tested positive for diarrhoeagenic E. coli. Fifty-eight percent (58%) of water samples were without health risks, 17% were low risk and 25% could cause infection according to the South African water quality standards. This study indicated the importance of the role of the Municipalities and the maintenance plans that need to ensure that all boreholes are functional and provide safe drinking water to the rural communities.

scholarly journals Occurrence of Pepper Mild Mottle Virus in Drinking Water Sources in Japan

2013 ◽  
Vol 79 (23) ◽  
pp. 7413-7418 ◽  
Author(s):  
Eiji Haramoto ◽  
Masaaki Kitajima ◽  
Naohiro Kishida ◽  
Yoshiaki Konno ◽  
Hiroyuki Katayama ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Rna Extraction ◽  
Water Sources ◽  
Mottle Virus ◽  
Environmental Waters ◽  
Pepper Mild Mottle Virus ◽  
Drinking Water Sources ◽  
Geographical Regions ◽  
Significant Difference

ABSTRACTPepper mild mottle virus (PMMoV) is a plant virus that has been recently proposed as a potential indicator of human fecal contamination of environmental waters; however, information on its geographical occurrence in surface water is still limited. We aimed to determine the seasonal and geographic occurrence of PMMoV in drinking water sources all over Japan. Between July 2008 and February 2011, 184 source water samples were collected from 30 drinking water treatment plants (DWTPs); viruses from 1 to 2 liters of each sample were concentrated by using an electronegative membrane, followed by RNA extraction and reverse transcription. Using quantitative PCR, PMMoV was detected in 140 (76%) samples, with a concentration ranging from 2.03 × 103to 2.90 × 106copies/liter. At least one of the samples from 27 DWTPs (n= 4 or 8) was positive for PMMoV; samples from 10 of these DWTPs were always contaminated. There was a significant difference in the occurrence of PMMoV among geographical regions but not a seasonal difference. PMMoV was frequently detected in samples that were negative for human enteric virus orEscherichia coli. A phylogenetic analysis based on the partial nucleotide sequences of the PMMoV coat protein gene in 12 water samples from 9 DWTPs indicated that there are genetically diverse PMMoV strains present in drinking water sources in Japan. To our knowledge, this is the first study to demonstrate the occurrence of PMMoV in environmental waters across wide geographical regions.

scholarly journals Assessment of the chemical quality of drinking water in Cambodia

2006 ◽  
Vol 5 (1) ◽  
pp. 101-116 ◽  
Author(s):  
Peter R. Feldman ◽  
Jan-Willem Rosenboom ◽  
Mao Saray ◽  
Chea Samnang ◽  
Peng Navuth ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Supply ◽  
Ground Water ◽  
Rural Areas ◽  
Rapid Assessment ◽  
Water Sources ◽  
Health Concern ◽  
Chemical Quality ◽  
Drinking Water Sources

Most water supply programmes in Cambodia have focused on providing access to bacteriologically safe water, an approach which has led to an increasing reliance on ground water, especially in rural areas. However, there have been very few data collected on the chemical quality of the nation's drinking water sources, and few water supply programmes have the capacity to assess chemical quality. The study was designed to address this data gap by conducting a low-cost, rapid assessment of drinking water sources nationwide to determine whether there were any chemicals of concern in Cambodian water supply sources. Results of the assessment confirm that there are several parameters of health and aesthetic concern; dissolved arsenic is the most significant. Elevated arsenic levels (some exceeding 500 μg l-1) were detected in aquifers of moderate depth in several highly populated areas, confirming that further investigation of the occurrence of arsenic contamination in Cambodia is warranted. Other chemicals of health concern include nitrate, nitrite, fluoride and manganese. Additionally, many ground water sources are negatively impacted by parameters of aesthetic concern, such as iron, manganese, hardness and total dissolved solids. Elevated levels of these parameters have caused consumers to reject newly installed water supplies, often in favour of surface water sources that are bacteriologically unsafe.

scholarly journals KARAKTERISTIK RUMAH TANGGA PENGGUNA AIR MINUM KEMASAN DAN ISI ULANG DI INDONESIA

2019 ◽  
Vol 17 (3) ◽  
pp. 155-164
Author(s):  
Elsa Elsi ◽  
Sahat P Manalu ◽  
Dasuki Dasuki ◽  
Aria Kusuma
Keyword(s):  
Drinking Water ◽  
Travel Time ◽  
Water Source ◽  
Water Sources ◽  
Bottled Water ◽  
Drinking Water Source ◽  
Clean Water ◽  
Cross Sectional ◽  
Source Characteristics ◽  
Drinking Water Sources

ABSTRACT One of SDG's 2030 targets is that households have access to drinking water. The phenomenon is some of community use bottled water/refill as drinking water. The aim of this article are knowing household proportion with improve clean water source and water collecting time that use bottled water/refill drinking water, and relation of clean water source characteristics with using bottle/refill drinking water. Research design is cross sectional. Dependent variable is household drinking water sources type, independents are household clean water source characteristics, and travel time collecting water. Bivariate data analysis was carried out to analyze the relationship between the proportion of bottled / refilled water users by households with adequate water sources and households that use inappropriate water sources. The results showed that proportion of households with unimproved drinking water sources, unimproved clean water source and unimproved water collecting time are greater using bottled/refill  drinking, each (7,6%) and (26,7%). There are statistic significances relation between household with unimproved drinking water source, unimproved travel time collecting water with using bottled/ relill drinking water (p = 0,000). Also found statistic significances between household with improved drinking water source, unimproved clean water source and unimproved travel time collecting water with using bottled/ relill drinking water (p = 0,000). Keywords: Bottled water, refill drinking water, improved water   ABSTRAK Salah satu target SDG’s 2030 adalah rumah tangga memiliki akses terhadap air minum. Sebagian masyarakat menggunakan air minum kemasan/ isi ulang sebagai air minum. Artikel ini bertujuan untuk mengetahui karakteristik pengguna air minum kemasan/isi ulang sebagai sumber air utama. Desain penelitian adalah potong lintang. Variabel terikat adalah jenis sumber air minum rumah tangga, sedangkan variabel bebas terdiri dari karakteristik sumber air utama dan waktu yang diperlukan untuk mengambil air. Analisis data secara bivariat dilakukan untuk menganalisis hubungan antara proporsi pengguna air minum kemasan/isi ulang oleh rumah tangga dengan sumber air layak dengan rumah tangga yang menggunakan sumber air tidak  layak.  Hasil menunjukkan bahwa proporsi pengguna air minum kemasan/isi ulang oleh  rumah tangga dengan sumber air minum yang belum layak  lebih tinggi diibandingkan dengan rumah tangga dengan sumber air minum layak, yaitu masing-masing (7,6%) dan (26,7%). Terdapat hubungan bermakna antara rumah tangga dengan sumber air minum belum layak, dengan waktu tempuh pengambilan air belum layak terhadap penggunaan air minum kemasan/ isi ulang (p=0,000).  Pada rumah tangga dengan  sumber air minum layak, sumber air utama belum layak dan waktu pengambilan belum layak juga ditemukan hubungan bermakna secara statistik terhadap penggunaan air minum kemasan/ isi ulang (p=0,000). Kata kunci: Air kemasan, air minum isi ulang, air layak

scholarly journals Domestic drinking water management: Quality assessment in Oforikrom municipality, Ghana

2021 ◽  
Vol 104 (3) ◽  
pp. 003685042110359
Author(s):  
Eugene Appiah-Effah ◽  
Emmanuel Nketiah Ahenkorah ◽  
Godwin Armstrong Duku ◽  
Kwabena Biritwum Nyarko
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Samples ◽  
Water Quality Management ◽  
Water Sources ◽  
Quality Analysis ◽  
Water Quality Analysis ◽  
Microbial Water Quality ◽  
Drinking Water Sources

Drinking water in Ghana is estimated at 79%, but this only represents the proportion of the population with access to improved drinking water sources without regard to the quality of water consumed. This study investigated the quality of household drinking water sources in the Oforikrom municipality where potable water requirements are on the rise due to an ever-increasing population. Both quantitative and qualitative methods were employed in this study. One Hundred households were randomly selected and interviewed on the available options for drinking water and household water treatment and safe storage. A total of 52 points of collection (POC) and 97 points of use (POU) water samples from households were collected for physicochemical and microbial water quality analysis. Amongst the available drinking water options, sachet water (46%) was mostly consumed by households. Water quality analysis revealed that the physicochemical parameters of all sampled drinking water sources were within the Ghana Standards Authority (GSA) recommended values expected for pH (ranging from 4.50 to 7.50). For the drinking water sources, bottled (100%, n = 2) and sachet water (91%, n = 41) showed relatively good microbial water quality. Generally, POC water samples showed an improved microbial water quality in comparison to POU water samples. About 38% ( n = 8) of the households practicing water quality management, were still exposed to unsafe drinking water sources. Households should practice good water quality management at the domestic level to ensure access to safe drinking water. This may include the use of chlorine-based disinfectants to frequently disinfect boreholes, wells and storage facilities at homes.

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