Preparation of a novel positively charged nanofiltration composite membrane incorporated with silver nanoparticles for pharmaceuticals and personal care product rejection and antibacterial properties

A novel positively charged N-[(2-hydroxy-3-trimethylammonium)propyl] chloride chitosan (HTCC)-Ag/polyethersulfone (PES) composite nanofiltration membrane was easily prepared by coating the active layer, HTCC, onto PES as the support through epichlorohydrin as the cross-linking reagent and nano-Ag particles as the introduced inorganic components. Scanning election microscopy, X-ray photoelectron spectroscopy, atomic force microscopy, and X-ray diffraction were employed to characterize the morphology of the resultant membranes, of which the molecular weight cut-off was about 941 Da. At 25 °C, the pure water permeability is 16.27 L/h·m2·MPa. Our results showed that the rejection of pharmaceuticals and personal care products (PPCPs) followed the sequence: atenolol > carbamazepine > ibuprofen, confirming that the membranes were positively charged. The antibacterial properties of the membranes were compared to elucidate the existence of Ag nanoparticles which help to improve antibacterial activity against Gram-negative Escherichia coli (DH5α, Rosetta) and Gram-positive Bacillus subtilis. The inhibition zone diameters of HTCC-Ag/PES membranes towards E. coli DH5α, E. coli Rosetta and Bacillus subtilis were 17.77, 16.18, and 15.44 mm, respectively. It was found that HTCC-Ag/PES membrane has a better antibacterial activity against E. coli than against Bacillus subtilis, especially for E. coli DH5α.

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The Effects of Copper Addition on The Structure and Antibacterial Properties of Biomedical Glasses

10.1101/2020.01.24.918524 ◽

2020 ◽

Author(s):

Leyla Mojtabavi ◽

Amir Razavi

Keyword(s):

Antibacterial Properties ◽

Inhibition Zone ◽

X Ray Diffraction ◽

Network Modifier ◽

X Ray ◽

E Coli ◽

Copper Addition ◽

Xrd Patterns ◽

Copper Incorporation ◽

Glass Compositions

AbstractIn this work, we studied the effects of copper incorporation in the composition of bioactive glass. Three different glass compositions were synthesized with 0, 3, and 6 mol% of copper addition. X-Ray Diffraction (XRD) patterns confirmed that an amorphous microstructure was obtained for all three glass compositions. Results from Differential Thermal Analysis (DTA) showed that the copper addition in the glass lowers the glass transition temperature, from 646°C to 590°C when added at 6 mol%. X-ray Photoelectron (XPS) survey and high-resolution scans were performed to study the structural effects of copper addition in the glass. Results indicated that the incorporation of copper changes the ratio of bridging to non-birding oxygens in the structure. Glasses were further analyzed for their structure with Nuclear Magnetic Resonance (NMR) spectroscopy, which indicated that copper acts as a network modifier in the glass composition and copper-containing glasses show a less connected microstructure. Antibacterial efficacy of the glasses was analyzed against E. coli and S. epidermis. Copper-containing glasses showed a significantly higher inhibition zone compared to control glass. The glass with 6 mol% copper, exhibited inhibition zones of 9 and 16mm against E. coli and S. epidermis bacteria, respectively.

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Fabrication of cotton fabrics with durable antibacterial activities finishing by Ag nanoparticles

Textile Research Journal ◽

10.1177/0040517518758002 ◽

2018 ◽

Vol 89 (5) ◽

pp. 867-880 ◽

Cited By ~ 18

Author(s):

Yunping Wu ◽

Yan Yang ◽

Zhijie Zhang ◽

Zhihua Wang ◽

Yanbao Zhao ◽

...

Keyword(s):

Photoelectron Spectroscopy ◽

Inductively Coupled Plasma ◽

Antibacterial Properties ◽

Antimicrobial Activities ◽

Cotton Fabrics ◽

Synthesis Process ◽

Ag Nps ◽

X Ray ◽

E Coli ◽

Fabric Surface

In this paper, we propose a facile and mild route to prepare size-tunable silver nanoparticles (Ag NPs) and their finishing application on fabrication of antibacterial cotton fabrics. The as-prepared Ag NPs, with an average particles size of 2.3 nm, show the minimal inhibitory concentration of 7.8 µg/mL and the minimum bactericidal concentration of 15.6 µg/mL, respectively. In this study, sodium citrate served as a stabilizing agent to prevent Ag NP agglomeration in the synthesis process, and citric acid acted as a binder to fix Ag NPs on the cotton fabrics through chemical bonds in the finishing process. The results of Fourier transform infrared spectroscopy, ultraviolet-visible spectroscopy (UV-vis), X-ray diffraction, X-ray photoelectron spectroscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy demonstrate that Ag NPs have been fixed and well dispersed on the cotton fabric surface. Ag contents in the hybrid fabrics were measured by the techniques of inductively coupled plasma atomic emission spectroscopy and UV-vis, and the antibacterial properties of hybrid fabrics were tested by the shake flask and agar diffusion plate method. It is found that the Ag NP coated cotton fabrics exhibit excellent antimicrobial activities against both the Gram-negative bacterium of Escherichia coli (E. coli) and the Gram-positive bacterium of Staphylococcus aureus ( S. aureus). The percentages of reduction bacteria remain at 91.8% and 98.7% for S. aureus and E. coli, respectively, even after 50 cycles of consecutive laundering, which indicates that the antibiotic performance of the as-fabricated hybrid fabrics is also durable.

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FRAKSI ETANOL, KLOROFORM, DAN N-HEKSAN BUNGA KAMBOJA PUTIH (Plumeria acuminata L.) SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli dan Staphylococcus aureus DENGANBIOAUTOGRAFI

JFL : Jurnal Farmasi Lampung ◽

10.37090/jfl.v8i2.147 ◽

1970 ◽

pp. 111-122

Author(s):

Yuli Wahyu Trimulyani

Keyword(s):

Antibacterial Activity ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Bacterial Suspension ◽

Chloroform Fraction ◽

Contact Method ◽

Fractionation Process ◽

E Coli ◽

Layer Chromatography ◽

Ethanol Fraction

Frangipani flowers (Plumeria acuminata L.) Flower is one of the plants that has antibacterial properties. Compounds that act as antibacterial are flavonoids and tannins. The purpose of this study was to prove the antibacterial activity of ethanol, chloroform, and n-hexane fractions of Frangipani flowers (Plumeria acuminata L.) flower as antibacterial against E. coli and S. aureus with Bioautography.The process of extracting plumeria acuminata L. flowers was carried out by maceration method using 70% ethanol. The extract was continued with the fractionation process with ethanol, n-hexane and chloroform solvents. Antibacterial activity testing using the well method with concentrations of 20%, 40%, 60%, 80%, 100%, positive chloramphenicol control, and negative aquadest control. The compound content test used the Thin Layer Chromatography (TLC) method with the mobile phase of chloroform: methanol: water (2:5:3) (v/v).Bioautography test using the contact method, namely the elution TLC plate, was placed on the NA medium containing a bacterial suspension for 3 hours. The biggest antibacterial test results of ethanol fraction of plumeria acuminata L. flowers were at a concentration of 100% with a diameter of inhibition zone of 13.55 mm in E. coli while 14.15 mm S. aureus. The results of TLC showed the presence of flavonoids with a price of Rf 0.70 and tannin at a price of Rf 0.68. Bioautography results showed a inhibition zone with a price of Rf 0.70 in E. coli and S. aureus which are flavonoid compounds. Based on the results of the research that has been done it can be concluded that the ethanol fraction of white frangipani flowers has antibacterial activity against E.coli and S. aureus, while the chloroform fraction and n-hexane fraction do not have antibacterial activity. The active compounds that act as antibacterial are flavonoids and tannin, but the ones that provide antibacterial effects are flavonoids with an Rf value of 0.70. Keywords: Bioautography,Escherichiacoli, Frangipani flowers, Staphylococcusaureus, TLC

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Gram-scale synthesis of splat-shaped Ag–TiO2 nanocomposites for enhanced antimicrobial properties

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.11.96 ◽

2020 ◽

Vol 11 ◽

pp. 1119-1125

Author(s):

Mohammad Jaber ◽

Asim Mushtaq ◽

Kebiao Zhang ◽

Jindan Wu ◽

Dandan Luo ◽

...

Keyword(s):

Antibacterial Activity ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Inhibition Zone ◽

Multidrug Resistant ◽

Bacterial Strains ◽

Gram Negative ◽

E Coli ◽

Contagious Diseases ◽

Good Biocompatibility

The control over contagious diseases caused by pathogenic organisms has become a serious health issue. The extensive usage of antibiotics has led to the development of multidrug-resistant bacterial strains. In this regard, metal-oxide-based antibacterial nanomaterials have received potential research interest due to the efficient prevention of microorganism growth. In this study, splat-shaped Ag–TiO2 nanocomposites (NCs) were synthesized on the gram scale and the enhanced antibacterial properties of TiO2 in the presence of silver were examined. The formation of Ag–TiO2 NCs was analyzed through various characterization techniques. The cell viability experimental results demonstrated that the Ag–TiO2 NCs have good biocompatibility. The antibacterial activity of the prepared Ag–TiO2 NCs was tested against the Gram-positive Staphylococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacterial strains. The Ag–TiO2 NCs exhibited promising and superior antibacterial properties compared to TiO2 nanospheres as confirmed by the bacterial growth and inhibition zone. The improvement in the antibacterial activity was attributed to the synergistic effect of the hybrid nature of TiO2 nanoparticles in the presence of Ag.

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Antibacterial Activity of Visible Light-Activated TiO2 Thin Films with Low Level of Fe Doping

Advances in Materials Science and Engineering ◽

10.1155/2019/5819805 ◽

2019 ◽

Vol 2019 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Dan Meng ◽

Xiuhua Liu ◽

Yun Xie ◽

Yang Du ◽

Yong Yang ◽

...

Keyword(s):

Thin Films ◽

Raman Spectroscopy ◽

Antibacterial Activity ◽

Visible Light ◽

Photoelectron Spectroscopy ◽

Sol Gel ◽

Antibacterial Properties ◽

Dip Coating ◽

Tio2 Thin Films ◽

X Ray

Development of effective antibacterial visible light-activated photocatalytic materials in industries including wastewater treatment and food industry has attracted increasing attention. In this work, Fe-doped TiO2 thin films with different doping levels on a glass substrate were prepared by the sol-gel dip-coating method. The as-prepared films were characterized by Raman spectroscopy, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and atomic force microscope (AFM). Raman spectroscopy and XRD results show the crystalline phase of titanium dioxide was anatase, and the range of the crystal size for the films was 19.24–22.24 nm. XPS results indicate that iron was in the form of Fe3+ in Fe-doped TiO2 films. Regarding the antibacterial properties of TiO2 films, the order of antibacterial activity of TiO2 films was 0.1 at% Fe > 0.5 at% Fe > 1.0 at% TiO2 > bare TiO2 > 2.0 at% Fe > 3.45 at% Fe. 0.1 at% of Fe is the optimum dopant ratio related to antibacterial activity. 0.1 at% Fe-doped TiO2 film is highly efficient in inactivating E. coli under 3 h of visible light irradiation, and it remains efficient even in real dye waste water.

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Microwave-Assisted Rapid Synthesis of Eu(OH)3/RGO Nanocomposites and Enhancement of Their Antibacterial Activity against Escherichia coli

Materials ◽

10.3390/ma15010043 ◽

2021 ◽

Vol 15 (1) ◽

pp. 43

Author(s):

Kun-Yauh Shih ◽

Shiou-Ching Yu

Keyword(s):

Antibacterial Activity ◽

Inhibition Zone ◽

X Ray Diffraction ◽

Inhibition Zone Diameter ◽

X Ray ◽

E Coli ◽

Inhibition Effect ◽

Microwave Assisted ◽

High Antibacterial Activity ◽

The Eu

Nanomaterials with high antibacterial activity and low cytotoxicity have attracted extensive attention from scientists. In this study, europium (III) hydroxide (Eu(OH)3)/reduced graphene oxide (RGO) nanocomposites were synthesized using a rapid, one-step method, and their antibacterial activity against Escherichia coli (E. coli) was investigated using the synergistic effect of the antibacterial activity between Eu and graphene oxide (GO). The Eu(OH)3/RGO nanocomposites were prepared using a microwave-assisted synthesis method and characterized using X-ray diffraction, scanning electron microscopy, photoluminescence spectroscopy, Raman spectroscopy, and Fourier-transform infrared spectroscopy. Raman sprectroscopy and X-ray diffraction confirmed the pure hexagonal phase structure of the nanocomposites. Further, the antibacterial properties of Eu(OH)3/RGO were investigated using the minimum inhibitory concentration assay, colony counting method, inhibition zone diameter, and optical density measurements. The results revealed that the Eu(OH)3/RGO exhibited a superior inhibition effect against E. coli and a larger inhibition zone diameter compared to RGO and Eu(OH)3. Further, the reusability test revealed that Eu(OH)3/RGO nanocomposite retained above 98% of its bacterial inhibition effect after seven consecutive applications. The high antibacterial activity of the Eu(OH)3/RGO nanocomposite could be attributed to the release of Eu3+ ions from the nanocomposite and the sharp edge of RGO. These results indicated the potential bactericidal applications of the Eu(OH)3/RGO nanocomposite.

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ANTIBACTERIAL ACTIVITY OF THE MEDICINAL PLANT, ACALYPHA FRUTICOSA FORSSK.

Kongunadu Research Journal ◽

10.26524/krj149 ◽

2016 ◽

Vol 3 (2) ◽

pp. 72-74

Author(s):

Thambiraj J

Keyword(s):

Antibacterial Activity ◽

Bacillus Subtilis ◽

Medicinal Plant ◽

Pathogenic Bacteria ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Diffusion Method ◽

Root Extracts ◽

Bacterium Bacillus Subtilis ◽

Bacterium Bacillus

The present study is to confirm the antibacterial efficacy of root extracts of the folklore medicinal plant species, Acalypha fruticosa by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against ten human pathogenic bacteria viz., Pseudomonas aeruginosa, P. stutzeri,Escherichia coli, Micrococcus sp., Lactobacillus sp., Servatia sp., Moraxetta sp., Bacillus subtilis, B. thuriengensis, and Klebsiella pneumoniae for assessing the antibacterial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antibacterial activity against the tested pathogens. However, the methanol extract exhibited higher inhibition zone (21.83 mm) against the bacterium, Bacillus subtilis. This result supports the therapeutic importance of the species, Acalypha fruticosa in curing infectious diseases and encourages the extensive use of this species in health carepractices.

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Phytochemical Screening and Antibacterial Activity of Acorus calamus L. Extracts

Jurnal Biodjati ◽

10.15575/biodjati.v4i1.4235 ◽

2019 ◽

Vol 4 (1) ◽

pp. 68-78

Author(s):

Bayyinatul Muchtaromah ◽

Alfiah Hayati ◽

Erna Agustina

Keyword(s):

Antibacterial Activity ◽

Antibacterial Properties ◽

Ethanol Extract ◽

Inhibition Zone ◽

Common Species ◽

Chloroform Extract ◽

Hexane Extract ◽

Acorus Calamus ◽

E Coli ◽

Microdilution Method

Staphylococcus aureus and Escherichia coli are among the most common species of gram-positive and gram-negative bacteria, which cause vaginitis, in infertile women. The Calamus rhizome (Acorus calamus L.) is an Indonesian plant that has antibacterial properties that can be used to treat vaginitis and increase fertility. The aim of this study was to determine the phytochemical and antibacterial activity of the calamus rhizoma in polar, semi-polar and non-polar solvents in the growth of S. aureus and E. coli. The antibacterial activity test was in the form of inhibitory test using the Kirby-Bauer, Minimum Inhibi-tion Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by microdilution method with multilevel dilution (concentra-tions 50; 25; 12.5; 6.25; 3.13; 1.56; 0.78; and 0.39%). The screening results showed that ethanol and n-hexane extract contained alkaloids and triterpenoids, while chloroform extract was only triterpenoid. Chloroform extract produced the largest inhibition zone diameter of S. aureus and E. coli (7.26 and 3.28 mm), followed by ethanol extract (5.90 and 3.07 mm) and n-hexane extract (5.33 and 2.95 mm). The concentrations of 0.39 and 0.78% were the values of MIC and MBC for all three extracts, indicating that the extract of the calamus rhizome with several solvents in this study had the same antibacterial activity.

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Antibacterial Activity of Aloe Vera Gel-based Edible Coating with the addition of Gum Arabic and Ascorbic Acid

AJARCDE | Asian Journal of Applied Research for Community Development and Empowerment ◽

10.29165/ajarcde.v5i1.59 ◽

2021 ◽

Vol 5 (1) ◽

pp. 1-4

Author(s):

Ni Made Defy Janurianti ◽

I Made Supartha Utama ◽

Ida Bagus Wayan Gunam

Keyword(s):

Antibacterial Activity ◽

Aloe Vera ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Edible Coating ◽

Diffusion Method ◽

Heating Process ◽

Antibacterial Compounds ◽

E Coli ◽

Aloe Vera Gel

Aloe vera gel has antibacterial properties. The content of antibacterial compounds in aloe vera gel is saponins, anthraquinones, tannins, aloin, and acemannan. Aloe vera gel has the potential as an edible coating for food products. The Processing carried out goes through a heating process that does think to damage the antibacterial compounds in the aloe vera gel. This study aims to determine the antibacterial activity of aloe vera gel formulated as an edible coating on S. aureus, S. mutans, E. coli and K. pneumoniae bacteria. The research method used is the disc diffusion method. Based on the test results, pure aloe vera gel has a larger inhibition zone diameter than aloe vera gel processed into an edible coating. Diameter of pure aloe vera gel inhibition zone in S. aureus, S. mutans, E. coli, and K. pneumoniae bacteria. respectively 10.966 ± 0.573 mm; 11.806 ± 0.215 mm; 10,860 ± 0.675 mm, and 10.686 ± 0.081 mm. Aloe vera gel formulated into an edible coating with antibacterial ability, namely at a concentration of 100%, which has anti activity with the inhibition zone's diameter in S. aureus, S. mutans E. coli, and K. pneumoniae bacteria, respectively. is 10,470 ± 0.213 mm; 10,673 ± 0.127 mm; 10,113 ± 0.040 mm, and 9.676 ± 0.604 mm.

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A Facile Strategy to Fabricate Silver-Functionalized Superhydrophobic Cotton Fabrics with Long-Term Antibacterial Properties

10.21203/rs.3.rs-696268/v1 ◽

2021 ◽

Author(s):

Zhanpeng Ye ◽

Hui Rong ◽

Shuangyang Li ◽

Qing Ma ◽

Liandong Deng ◽

...

Keyword(s):

Antibacterial Activity ◽

Antibacterial Properties ◽

Inhibition Zone ◽

Dip Coating ◽

Hybrid Nanoparticles ◽

Preparation Process ◽

E Coli ◽

Wetting Properties ◽

Medical Materials

Abstract Silver-functionalized textiles, have attracted considerable attention as the most common protective medical materials. However, their poor antibacterial durability and relatively tedious preparation process limit their applications. In this study, a kind of multifunctional cotton fabric was prepared through simultaneous dip-coating of silver-silica hybrid nanoparticles (Ag-MSNs) and fluorinated MSNs (F-MSNs), leading to excellent antifouling due to superhydrophobicity and long-term antibacterial properties from the sustained release of Ag ions (Ag+). Detailed studies were performed to evaluate their structure and protective performance, especially the long-term antibacterial properties of the obtained fabrics through the inhibition zone experiment for 25 d. The as-prepared fabrics showed good non-wetting properties and sustained antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Further washing and abrasion experimentations demonstrated that the as-prepared fabrics had stable hydrophobicity and antibacterial properties, much more reliable than commercially derived antibacterial gauze. Considering the readily available inexpensive materials and simple preparation process as well as long-term and efficient sterilization effect, the strategy provided herein opens a new and facile way to fabricate multifunctional fabrics with robust hydrophobic and antibacterial activity for the future medical materials.

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